Rh1BG029400

Belongs to the CTL (choline transporter-like) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0002251
Physical Location & Seq
Reverse (-)
3995670 .. 4000235
4566 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG029400.1

Sequence Viewer

Length: 1479 bp
ATGGGTGCCGCAGAACCCGTAAAGTTTCCATCTTTTATTTTGGTTTGCTACCAATTGGCTGATTTCATGTGTGTGAGATGCATTAAAGGTCACAACTTTGGTTTTTATGTGCAGGTGGAGAGAGAAAGTGAAGAGGTTGAGGCGAGGGAGAGAGATGAGAGGGAGGAGGAGGAAGAGGAGGAGGAGAGGAGAGAGCTGGAAGTGAGAGATGTGGAGAAAGTTGAGGTGTTGGGTTTTGAACAACAGAGCAATATTAATCATGATCGGAGTAGTCAGGGAGATGTTCATGTGTCAATGTTGCAGAGGCTTAATCCTACAAACCCTTTGAGAATTGCTATCAATACTAAAACCAGAAGCGCTGCTCCTCCTCCTCCTCATCGGTACCAACAGGCCCAAAATGGCAGAGTTGGTACTACTCCATCTCCATCTCTATCTGATTTCTCTCATCAGTCTCACACAACTCCTTCTCACACAACTCCTCCTCCTCCTCCTCCTCAGCATTCTCAGCCTCGCTCTATTCCAACCCCTCAACCAGCGATTACAACACTGAACTCAAGAGCCTACACCAACAAGATGTCTTTGTTCATTTTCGTTGTGCACATGGTGGCCGCTATTGGGCTAGTTGGTTTTCTGATATTCAAGGGAGTTCAGGGTCTGATAGAAAATTCAGGCAGTATTTCGAGAAAGGAGAAAAGGGTGTTAAAGTTTTTTCTACCACAAGTGGAGGCTGCATCTCTTATGAGCATTACTCTTGCATTTGCATGGCAAAAGGCATTTAGGAAATGGCCTACATTTATGGTTCATTTCATACTATGGAACACTTTTGTCATGAGTTTATCTGCTGGAATCCTACTAATTTGTTTCCAAATGCCCACCACTACTGGCGTGGGAGTCTGTTTCATTGGCTTTGCCATAGGGAATAGCTTGTACGCTTGTTGGATCACGCCGCGGATAGGATTTTGTATCAAGATATTGATCAAGTCGCTCGAACCTGTATCAAAGTTCCGTGATTTGAATCAACCCATTTACTGGATGCTTGGGGTCGGATTTTTGTGGATGTCTCTATGGATTTTAGGAGTGATTGGAGCCTTGAATTTCTACTTTCCTCCACTAGTCATAATTTCATTGGTATTGAGCCTGATGTGGACAGCCGAAGTAATGAGGAATGTAGCTAATATAACTGTGAGTAGGGTGATTGCTCTATATTATCTCAGAGGAATGCAGTCCAATACTCAGTTTTGTTTCCAGAGAGCCTTGAGTAAAAATCTTGGAAGTGCTTGTTTTGGATCTCTGTTTGTGCCAGCAATTGAAGCGCTGAGAATTGTTGCTCGAGGTTTAAATCTACTTGAGGGAGAGGATGAGTTCATGTTTTCTTGTGCGCATTGCTGTCTCCATGTCATGCAGTGCATCTTCCGATATGGCAACGGCTGGGCCTTTGTTCAGGTACCTTTACTCAAGTCATTAGCAATTTCTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Choline_transpo

Protein Analysis

492

Amino Acids

55.46

Weight (kDa)

8.64

Isoelectric Point (pI)

51.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Choline_transpo PF04515 299 - 482 9.8e-19 Plasma-membrane choline transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 103
Acc16I TGCGCA 1 cut(s) 1380
Acc36I ACCTGC 1 cut(s) 103
Acc65I GGTACC 2 cut(s) 381, 1444
AccB1I GGYRCC 3 cut(s) 5, 381, 1444
AccB7I CCANNNNNTGG 1 cut(s) 1029
AccII CGCG 1 cut(s) 949
AciI CCGC 4 cut(s) 9, 609, 947, 949
AclWI GGATC 2 cut(s) 947, 1294
AcoI YGGCCR 1 cut(s) 606
AcsI RAATTY 2 cut(s) 664, 1093
AdeI CACNNNGTG 1 cut(s) 604
AfaI GTAC 4 cut(s) 383, 412, 929, 1446
AfeI AGCGCT 2 cut(s) 358, 1314
AfiI CCNNNNNNNGG 3 cut(s) 615, 953, 1029
AgsI TTSAA 5 cut(s) 239, 640, 1015, 1093, 1310
AhlI ACTAGT 1 cut(s) 1111
AluBI AGCT 3 cut(s) 196, 924, 1172
AluI AGCT 3 cut(s) 196, 924, 1172
Alw21I GWGCWC 1 cut(s) 600
Alw26I GTCTC 3 cut(s) 456, 1065, 1394
Alw44I GTGCAC 1 cut(s) 596
AlwI GGATC 2 cut(s) 947, 1294
AlwNI CAGNNNCTG 1 cut(s) 655
Ama87I CYCGRG 1 cut(s) 1329
Aor51HI AGCGCT 2 cut(s) 358, 1314
AoxI GGCC 4 cut(s) 390, 606, 785, 1431
ApaLI GTGCAC 1 cut(s) 596
ApeKI GCWGC 2 cut(s) 359, 728
ApoI RAATTY 2 cut(s) 664, 1093
AseI ATTAAT 1 cut(s) 255
Asp718I GGTACC 2 cut(s) 381, 1444
AspLEI GCGC 3 cut(s) 359, 1315, 1381
AspS9I GGNCC 2 cut(s) 391, 1431
AsuHPI GGTGA 1 cut(s) 1204
AvaI CYCGRG 1 cut(s) 1329
BaeGI GKGCMC 1 cut(s) 600
BanI GGYRCC 3 cut(s) 5, 381, 1444
Bbv12I GWGCWC 1 cut(s) 600
BbvCI CCTCAGC 1 cut(s) 495
BbvI GCAGC 2 cut(s) 346, 715
BccI CCATC 3 cut(s) 37, 427, 433
BceAI ACGGC 1 cut(s) 1441
BclI TGATCA 1 cut(s) 975
BcoDI GTCTC 3 cut(s) 456, 1065, 1394
BcuI ACTAGT 1 cut(s) 1111
BfaI CTAG 2 cut(s) 620, 1112
BfoI RGCGCY 2 cut(s) 360, 1316
BfuAI ACCTGC 1 cut(s) 103
BisI GCNGC 5 cut(s) 9, 360, 609, 729, 947
BlsI GCNGC 5 cut(s) 10, 361, 610, 730, 948
BmeT110I CYCGRG 1 cut(s) 1329
BmgT120I GGNCC 2 cut(s) 391, 1431
BmiI GGNNCC 4 cut(s) 7, 383, 1087, 1446
BmsI GCATC 4 cut(s) 68, 740, 1023, 1416
BplI GAGNNNNNCTC 2 cut(s) 733, 765
Bpu10I CCTNAGC 1 cut(s) 495
BpuEI CTTGAG 4 cut(s) 538, 1276, 1367, 1439
BsaBI GATNNNNATC 2 cut(s) 974, 1014
BsaJI CCNNGG 1 cut(s) 947
BsaXI ACNNNNNCTCC 6 cut(s) 406, 436, 463, 466, 493, 496
Bsc4I CCNNNNNNNGG 3 cut(s) 615, 953, 1029
Bse1I ACTGG 2 cut(s) 886, 1034
Bse3DI GCAATG 1 cut(s) 1381
Bse8I GATNNNNATC 2 cut(s) 974, 1014
BseDI CCNNGG 1 cut(s) 947
BseGI GGATG 3 cut(s) 1038, 1062, 1363
BseJI GATNNNNATC 2 cut(s) 974, 1014
BseLI CCNNNNNNNGG 3 cut(s) 615, 953, 1029
BseMI GCAATG 1 cut(s) 1381
BseMII CTCAG 5 cut(s) 509, 518, 1225, 1247, 1307
BseNI ACTGG 2 cut(s) 886, 1034
BseSI GKGCMC 1 cut(s) 600
BseXI GCAGC 2 cut(s) 346, 715
BseYI CCCAGC 1 cut(s) 1428
BsgI GTGCAG 1 cut(s) 131
Bsh1236I CGCG 1 cut(s) 949
BshFI GGCC 4 cut(s) 392, 608, 787, 1433
BshNI GGYRCC 3 cut(s) 5, 381, 1444
BsiHKAI GWGCWC 1 cut(s) 600
BsiHKCI CYCGRG 1 cut(s) 1329
BslI CCNNNNNNNGG 3 cut(s) 615, 953, 1029
BsmAI GTCTC 3 cut(s) 456, 1065, 1394
BsmI GAATGC 2 cut(s) 499, 1224
BsnI GGCC 4 cut(s) 392, 608, 787, 1433
BsoBI CYCGRG 1 cut(s) 1329
Bsp1286I GDGCHC 1 cut(s) 600
Bsp143I GATC 4 cut(s) 262, 939, 975, 1286
BspACI CCGC 4 cut(s) 9, 609, 947, 949
BspANI GGCC 4 cut(s) 392, 608, 787, 1433
BspCNI CTCAG 5 cut(s) 508, 517, 1224, 1246, 1308
BspFNI CGCG 1 cut(s) 949
BspHI TCATGA 2 cut(s) 259, 828
BspLI GGNNCC 4 cut(s) 7, 383, 1087, 1446
BspMI ACCTGC 1 cut(s) 103
BspPI GGATC 2 cut(s) 947, 1294
BspT107I GGYRCC 3 cut(s) 5, 381, 1444
BsrDI GCAATG 1 cut(s) 1381
BsrI ACTGG 2 cut(s) 886, 1034
BssECI CCNNGG 1 cut(s) 947
BssMI GATC 4 cut(s) 262, 939, 975, 1286
Bst4CI ACNGT 1 cut(s) 1183
Bst6I CTCTTC 2 cut(s) 126, 168
BstC8I GCNNGC 1 cut(s) 1302
BstDEI CTNAG 5 cut(s) 495, 504, 1211, 1233, 1316
BstDSI CCRYGG 1 cut(s) 947
BstF5I GGATG 3 cut(s) 1038, 1062, 1363
BstFNI CGCG 1 cut(s) 949
BstH2I RGCGCY 2 cut(s) 360, 1316
BstHHI GCGC 3 cut(s) 359, 1315, 1381
BstKTI GATC 4 cut(s) 265, 942, 978, 1289
BstMAI GTCTC 3 cut(s) 456, 1065, 1394
BstMBI GATC 4 cut(s) 262, 939, 975, 1286
BstMWI GCNNNNNNNGC 2 cut(s) 505, 1310
BstSLI GKGCMC 1 cut(s) 600
BstUI CGCG 1 cut(s) 949
BstV1I GCAGC 2 cut(s) 346, 715
BstX2I RGATCY 1 cut(s) 1286
BstYI RGATCY 1 cut(s) 1286
BsuRI GGCC 4 cut(s) 392, 608, 787, 1433
BtgI CCRYGG 1 cut(s) 947
BtsCI GGATG 3 cut(s) 1038, 1062, 1363
BtsI GCAGTG 1 cut(s) 1409
BtsIMutI CAGTG 2 cut(s) 545, 1409
BveI ACCTGC 1 cut(s) 103
Cac8I GCNNGC 1 cut(s) 1302
CaiI CAGNNNCTG 1 cut(s) 655
CciI TCATGA 2 cut(s) 259, 828
CfoI GCGC 3 cut(s) 359, 1315, 1381
Cfr13I GGNCC 2 cut(s) 391, 1431
Cfr42I CCGCGG 1 cut(s) 950
Csp6I GTAC 4 cut(s) 382, 411, 928, 1445
CviAII CATG 9 cut(s) 67, 260, 287, 601, 762, 829, 1366, 1394, 1399
CviQI GTAC 4 cut(s) 382, 411, 928, 1445
DdeI CTNAG 5 cut(s) 495, 504, 1211, 1233, 1316
DpnI GATC 4 cut(s) 264, 941, 977, 1288
DpnII GATC 4 cut(s) 262, 939, 975, 1286
DraI TTTAAA 1 cut(s) 1338
DraIII CACNNNGTG 1 cut(s) 604
EaeI YGGCCR 1 cut(s) 606
Eam1104I CTCTTC 2 cut(s) 126, 168
EarI CTCTTC 2 cut(s) 126, 168
Eco47III AGCGCT 2 cut(s) 358, 1314
Eco88I CYCGRG 1 cut(s) 1329
EcoT22I ATGCAT 1 cut(s) 83
FaeI CATG 9 cut(s) 70, 263, 290, 604, 765, 832, 1369, 1397, 1402
FatI CATG 9 cut(s) 66, 259, 286, 600, 761, 828, 1365, 1393, 1398
FbaI TGATCA 1 cut(s) 975
Fnu4HI GCNGC 5 cut(s) 9, 360, 609, 729, 947
FokI GGATG 3 cut(s) 1045, 1069, 1370
Fsp4HI GCNGC 5 cut(s) 9, 360, 609, 729, 947
FspAI RTGCGCAY 1 cut(s) 1380
FspBI CTAG 2 cut(s) 620, 1112
FspI TGCGCA 1 cut(s) 1380
GlaI GCGC 3 cut(s) 358, 1314, 1380
GluI GCNGC 5 cut(s) 9, 360, 609, 729, 947
GsaI CCCAGC 1 cut(s) 1432
HaeII RGCGCY 2 cut(s) 360, 1316
HaeIII GGCC 4 cut(s) 392, 608, 787, 1433
HhaI GCGC 3 cut(s) 359, 1315, 1381
Hin1II CATG 9 cut(s) 70, 263, 290, 604, 765, 832, 1369, 1397, 1402
Hin6I GCGC 3 cut(s) 357, 1313, 1379
HinP1I GCGC 3 cut(s) 357, 1313, 1379
HinfI GANTC 3 cut(s) 846, 891, 1015
HphI GGTGA 1 cut(s) 1204
Hpy166II GTNNAC 2 cut(s) 598, 1146
Hpy188I TCNGA 7 cut(s) 267, 436, 633, 657, 1046, 1214, 1415
Hpy188III TCNNGA 7 cut(s) 260, 555, 681, 829, 967, 1246, 1476
Hpy8I GTNNAC 2 cut(s) 598, 1146
HpyAV CCTTC 1 cut(s) 474
HpyCH4III ACNGT 1 cut(s) 1183
HpyF10VI GCNNNNNNNGC 2 cut(s) 505, 1310
HpyF3I CTNAG 5 cut(s) 495, 504, 1211, 1233, 1316
Hsp92II CATG 9 cut(s) 70, 263, 290, 604, 765, 832, 1369, 1397, 1402
HspAI GCGC 3 cut(s) 357, 1313, 1379
KpnI GGTACC 2 cut(s) 385, 1448
Ksp22I TGATCA 1 cut(s) 975
KspI CCGCGG 1 cut(s) 950
Kzo9I GATC 4 cut(s) 262, 939, 975, 1286
LmnI GCTCC 2 cut(s) 367, 1085
Lsp1109I GCAGC 2 cut(s) 346, 715
LweI GCATC 4 cut(s) 68, 740, 1023, 1416
MaeI CTAG 2 cut(s) 620, 1112
MaeIII GTNAC 1 cut(s) 89
MalI GATC 4 cut(s) 264, 941, 977, 1288
MboI GATC 4 cut(s) 262, 939, 975, 1286
MboII GAAGA 4 cut(s) 143, 185, 1402, 1464
MfeI CAATTG 2 cut(s) 53, 1305
MflI RGATCY 1 cut(s) 1286
MhlI GDGCHC 1 cut(s) 600
MluCI AATT 9 cut(s) 53, 330, 664, 855, 1093, 1119, 1305, 1320, 1467
MlyI GAGTC 1 cut(s) 900
MmeI TCCRAC 3 cut(s) 545, 917, 1024
Mph1103I ATGCAT 1 cut(s) 83
MseI TTAA 5 cut(s) 84, 255, 309, 701, 1337
MslI CAYNNNNRTG 2 cut(s) 71, 760
MspA1I CMGCKG 1 cut(s) 949
MunI CAATTG 2 cut(s) 53, 1305
Mva1269I GAATGC 2 cut(s) 499, 1224
MvnI CGCG 1 cut(s) 949
MwoI GCNNNNNNNGC 2 cut(s) 505, 1310
NdeII GATC 4 cut(s) 262, 939, 975, 1286
NlaIII CATG 9 cut(s) 70, 263, 290, 604, 765, 832, 1369, 1397, 1402
NlaIV GGNNCC 4 cut(s) 7, 383, 1087, 1446
NmuCI GTSAC 1 cut(s) 89
NsbI TGCGCA 1 cut(s) 1380
NsiI ATGCAT 1 cut(s) 83
PaeR7I CTCGAG 1 cut(s) 1329
PagI TCATGA 2 cut(s) 259, 828
PaqCI CACCTGC 1 cut(s) 103
PctI GAATGC 2 cut(s) 499, 1224
PfeI GAWTC 2 cut(s) 846, 1015
PflMI CCANNNNNTGG 1 cut(s) 1029
PkrI GCNGC 5 cut(s) 10, 361, 610, 730, 948
PleI GAGTC 1 cut(s) 899
PpsI GAGTC 1 cut(s) 899
PshBI ATTAAT 1 cut(s) 255
PspFI CCCAGC 1 cut(s) 1428
PspN4I GGNNCC 4 cut(s) 7, 383, 1087, 1446
PspPI GGNCC 2 cut(s) 391, 1431
PspXI VCTCGAGB 1 cut(s) 1329
PstNI CAGNNNCTG 1 cut(s) 655
PsuI RGATCY 1 cut(s) 1286
RsaI GTAC 4 cut(s) 383, 412, 929, 1446
RsaNI GTAC 4 cut(s) 382, 411, 928, 1445
RseI CAYNNNNRTG 2 cut(s) 71, 760
SacII CCGCGG 1 cut(s) 950
SaqAI TTAA 5 cut(s) 84, 255, 309, 701, 1337
SatI GCNGC 5 cut(s) 9, 360, 609, 729, 947
Sau3AI GATC 4 cut(s) 262, 939, 975, 1286
Sau96I GGNCC 2 cut(s) 391, 1431
SchI GAGTC 1 cut(s) 900
SduI GDGCHC 1 cut(s) 600
SfaNI GCATC 4 cut(s) 68, 740, 1023, 1416
Sfr274I CTCGAG 1 cut(s) 1329
Sfr303I CCGCGG 1 cut(s) 950
SgrBI CCGCGG 1 cut(s) 950
SlaI CTCGAG 1 cut(s) 1329
SmiMI CAYNNNNRTG 2 cut(s) 71, 760
SmlI CTYRAG 5 cut(s) 553, 1255, 1329, 1346, 1454
SmoI CTYRAG 5 cut(s) 553, 1255, 1329, 1346, 1454
SpeI ACTAGT 1 cut(s) 1111
Sse9I AATT 9 cut(s) 53, 330, 664, 855, 1093, 1119, 1305, 1320, 1467
SsiI CCGC 4 cut(s) 9, 609, 947, 949
SspI AATATT 1 cut(s) 253
SspMI CTAG 2 cut(s) 620, 1112
TaaI ACNGT 1 cut(s) 1183
TaqI TCGA 3 cut(s) 680, 987, 1330
TasI AATT 9 cut(s) 53, 330, 664, 855, 1093, 1119, 1305, 1320, 1467
TauI GCSGC 3 cut(s) 11, 611, 949
TfiI GAWTC 2 cut(s) 846, 1015
Tru1I TTAA 5 cut(s) 84, 255, 309, 701, 1337
Tru9I TTAA 5 cut(s) 84, 255, 309, 701, 1337
TscAI CASTG 2 cut(s) 552, 1409
TseFI GTSAC 1 cut(s) 89
TseI GCWGC 2 cut(s) 359, 728
Tsp45I GTSAC 1 cut(s) 89
TspDTI ATGAA 8 cut(s) 55, 275, 574, 791, 796, 889, 1113, 1354
TspGWI ACGGA 1 cut(s) 995
TspRI CASTG 2 cut(s) 552, 1409
Van91I CCANNNNNTGG 1 cut(s) 1029
VneI GTGCAC 1 cut(s) 596
VspI ATTAAT 1 cut(s) 255
XapI RAATTY 2 cut(s) 664, 1093
XcmI CCANNNNNNNNNTGG 1 cut(s) 883
XhoI CTCGAG 1 cut(s) 1329
XspI CTAG 2 cut(s) 620, 1112
Zsp2I ATGCAT 1 cut(s) 83
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.