Rorug01G0021300

Belongs to the CTL (choline transporter-like) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0002251
Physical Location & Seq
Forward (+)
3461197 .. 3463821
2625 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0021300.1

Sequence Viewer

Length: 978 bp
ATGAGCTGCGGAGAAAGCAAGGTTGTGTGTGTGACGGGAGCATCTGGTTTCATAGCATCATGGCTGGTGAAGCTCTTATTGCAACGAGGTTATACTGTCAAAGCCACGGTTCGGGACCCAGACGATCAAAAGAAAACAGAACACCTACTCTCACTTGATGGAGCAAAGGAAAGGCTTCATTTGTTCAAAGCAGACTTGTTAGAAGAAGGGTCTTTTGATACGGCAATTGATGGATGTGAATGTGTTTTCCATACGGCATCCCCTGTCCTACTCTCAGTCTCATCTACTAATCCGCAGGCAGAATTAATTGACCCTGCTTTGAAAGGAACGCTTAATGTCCTCGGATCGTGTGTGAAGGTTCAGTCTATCAAAAGGGTGGTTATAACATCCTCTATGGCAGCAGTTCGATTTAATGGAAAACCTCTTGCTGCTGATGTAATAATCGATGAATCTTGGTTTTCAGATCCTGCTTTTTGTGAAAAAACAAAGCTTTGGTATATCCTTTCAAAGACATTAGCTGAGGAAGCTGCTTGGAAGTTTGCAAAAGAAAAAGGAATTGATATTGTTACGATAAATCCGGGATGGGTGATCGGCCCTCTCTTACAGCCAACTCTGAACTTGAGTGTGGAACTAGTTCTGAAACTCGTAAATGGGACCGAAAAGTTTCCCAACAGAACTTACAGACTTGTTGATGTTAGAGATGTTGCTATTGCACATATTCTAGCCTTTGAAAACCCATCAGCTAGTGGACGTTATTGTTTAGTTGGAAGCGTAAAACACTGTTCAGAGGTTGTGAAAATGTTGTACGAGATCTCCCCTGCTCTCAATCTTCCAGATAAATGTGCAGATGACAAGCCTTTCACACCAACCAACCAGGTATCCAAGGAAAGAACCCAAACTTTGGGTGTAAAGTATACTCCGCTTGAAGTGTCTCTGAAGGATACTGTTGAAAGTTTGAAGAACAAGAACTTCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Choline_transpo

Protein Analysis

325

Amino Acids

35.71

Weight (kDa)

6.94

Isoelectric Point (pI)

24.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 6 - 104 3.2e-09 NmrA-like family
Epimerase PF01370 9 - 249 5.6e-23 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 11 - 244 2e-19 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 11 - 131 2.5e-13 GDP-mannose 4,6 dehydratase
NAD_binding_4 PF07993 11 - 198 3e-12 Male sterility protein
NAD_binding_10 PF13460 13 - 136 3.9e-11 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 383
AccB7I CCANNNNNTGG 1 cut(s) 901
AccI GTMKAC 1 cut(s) 914
AciI CCGC 3 cut(s) 9, 293, 920
AclWI GGATC 2 cut(s) 352, 458
AcuI CTGAAG 1 cut(s) 956
AfaI GTAC 1 cut(s) 806
AfiI CCNNNNNNNGG 2 cut(s) 111, 901
AgsI TTSAA 7 cut(s) 187, 322, 507, 731, 926, 950, 958
AhlI ACTAGT 1 cut(s) 631
AjnI CCWGG 1 cut(s) 873
AluBI AGCT 6 cut(s) 6, 73, 490, 518, 527, 743
AluI AGCT 6 cut(s) 6, 73, 490, 518, 527, 743
Alw26I GTCTC 2 cut(s) 283, 936
AlwI GGATC 2 cut(s) 352, 458
AlwNI CAGNNNCTG 1 cut(s) 467
AoxI GGCC 1 cut(s) 592
ApeKI GCWGC 4 cut(s) 6, 398, 428, 527
AseI ATTAAT 1 cut(s) 305
Asp700I GAANNNNTTC 4 cut(s) 174, 633, 663, 971
AspS9I GGNCC 3 cut(s) 115, 593, 654
AsuC2I CCSGG 1 cut(s) 579
AsuHPI GGTGA 2 cut(s) 79, 598
AvaII GGWCC 2 cut(s) 115, 654
BbvCI CCTCAGC 1 cut(s) 519
BbvI GCAGC 3 cut(s) 410, 415, 514
BccI CCATC 4 cut(s) 152, 224, 576, 745
BceAI ACGGC 2 cut(s) 237, 270
BciT130I CCWGG 1 cut(s) 875
BciVI GTATCC 2 cut(s) 889, 934
BcnI CCSGG 1 cut(s) 579
BcoDI GTCTC 2 cut(s) 283, 936
BcuI ACTAGT 1 cut(s) 631
BfaI CTAG 3 cut(s) 632, 722, 744
BfuI GTATCC 2 cut(s) 889, 934
BglII AGATCT 1 cut(s) 810
BisI GCNGC 4 cut(s) 7, 399, 429, 528
BlsI GCNGC 4 cut(s) 8, 400, 430, 529
Bme1390I CCNGG 2 cut(s) 579, 875
Bme18I GGWCC 2 cut(s) 115, 654
BmgT120I GGNCC 3 cut(s) 115, 593, 654
BmiI GGNNCC 3 cut(s) 116, 117, 655
BmrFI CCNGG 2 cut(s) 579, 875
BmsI GCATC 3 cut(s) 50, 65, 266
Bpu10I CCTNAGC 1 cut(s) 519
BpuEI CTTGAG 1 cut(s) 640
BpuMI CCSGG 1 cut(s) 579
Bsa29I ATCGAT 1 cut(s) 444
BsaJI CCNNGG 3 cut(s) 105, 340, 882
BsaXI ACNNNNNCTCC 2 cut(s) 797, 827
Bsc4I CCNNNNNNNGG 2 cut(s) 111, 901
BseBI CCWGG 1 cut(s) 875
BseCI ATCGAT 1 cut(s) 444
BseDI CCNNGG 3 cut(s) 105, 340, 882
BseGI GGATG 4 cut(s) 239, 257, 386, 587
BseLI CCNNNNNNNGG 2 cut(s) 111, 901
BseMII CTCAG 2 cut(s) 288, 510
BseXI GCAGC 3 cut(s) 410, 415, 514
BsgI GTGCAG 1 cut(s) 864
BshFI GGCC 1 cut(s) 594
BshVI ATCGAT 1 cut(s) 444
BsiSI CCGG 1 cut(s) 578
BslFI GGGAC 2 cut(s) 128, 667
BslI CCNNNNNNNGG 2 cut(s) 111, 901
BsmAI GTCTC 2 cut(s) 283, 936
BsmFI GGGAC 2 cut(s) 128, 667
BsnI GGCC 1 cut(s) 594
Bsp143I GATC 5 cut(s) 124, 344, 463, 588, 810
BspACI CCGC 3 cut(s) 9, 293, 920
BspANI GGCC 1 cut(s) 594
BspCNI CTCAG 2 cut(s) 287, 511
BspDI ATCGAT 1 cut(s) 444
BspLI GGNNCC 3 cut(s) 116, 117, 655
BspPI GGATC 2 cut(s) 352, 458
BssECI CCNNGG 3 cut(s) 105, 340, 882
BssMI GATC 5 cut(s) 124, 344, 463, 588, 810
BssNAI GTATAC 1 cut(s) 915
BssT1I CCWWGG 1 cut(s) 882
Bst1107I GTATAC 1 cut(s) 915
Bst2UI CCWGG 1 cut(s) 875
Bst4CI ACNGT 4 cut(s) 97, 109, 782, 946
BstC8I GCNNGC 1 cut(s) 297
BstDEI CTNAG 2 cut(s) 274, 519
BstDSI CCRYGG 1 cut(s) 105
BstF5I GGATG 4 cut(s) 239, 257, 386, 587
BstKTI GATC 5 cut(s) 127, 347, 466, 591, 813
BstMAI GTCTC 2 cut(s) 283, 936
BstMBI GATC 5 cut(s) 124, 344, 463, 588, 810
BstMWI GCNNNNNNNGC 4 cut(s) 15, 70, 79, 524
BstNI CCWGG 1 cut(s) 875
BstSCI CCNGG 2 cut(s) 577, 873
BstV1I GCAGC 3 cut(s) 410, 415, 514
BstX2I RGATCY 2 cut(s) 463, 810
BstYI RGATCY 2 cut(s) 463, 810
BstZ17I GTATAC 1 cut(s) 915
Bsu15I ATCGAT 1 cut(s) 444
BsuI GTATCC 2 cut(s) 889, 934
BsuRI GGCC 1 cut(s) 594
BsuTUI ATCGAT 1 cut(s) 444
BtgI CCRYGG 1 cut(s) 105
BtsCI GGATG 4 cut(s) 239, 257, 386, 587
BtsIMutI CAGTG 1 cut(s) 778
Cac8I GCNNGC 1 cut(s) 297
CaiI CAGNNNCTG 1 cut(s) 467
Cfr13I GGNCC 3 cut(s) 115, 593, 654
ClaI ATCGAT 1 cut(s) 444
CsiI ACCWGGT 1 cut(s) 873
Csp6I GTAC 1 cut(s) 805
CviAII CATG 1 cut(s) 60
CviQI GTAC 1 cut(s) 805
DdeI CTNAG 2 cut(s) 274, 519
DpnI GATC 5 cut(s) 126, 346, 465, 590, 812
DpnII GATC 5 cut(s) 124, 344, 463, 588, 810
Eco130I CCWWGG 1 cut(s) 882
Eco47I GGWCC 2 cut(s) 115, 654
Eco57I CTGAAG 1 cut(s) 956
EcoO109I RGGNCCY 1 cut(s) 115
EcoRII CCWGG 1 cut(s) 873
EcoT14I CCWWGG 1 cut(s) 882
ErhI CCWWGG 1 cut(s) 882
FaeI CATG 1 cut(s) 63
FaiI YATR 9 cut(s) 53, 61, 93, 252, 383, 395, 498, 717, 915
FalI AAGNNNNNCTT 3 cut(s) 315, 347, 956
FaqI GGGAC 2 cut(s) 128, 667
FatI CATG 1 cut(s) 59
FblI GTMKAC 1 cut(s) 914
Fnu4HI GCNGC 4 cut(s) 7, 399, 429, 528
FokI GGATG 4 cut(s) 244, 246, 373, 594
Fsp4HI GCNGC 4 cut(s) 7, 399, 429, 528
FspBI CTAG 3 cut(s) 632, 722, 744
GluI GCNGC 4 cut(s) 7, 399, 429, 528
HaeIII GGCC 1 cut(s) 594
HapII CCGG 1 cut(s) 578
Hin1II CATG 1 cut(s) 63
HindIII AAGCTT 1 cut(s) 488
HinfI GANTC 1 cut(s) 449
HpaII CCGG 1 cut(s) 578
HphI GGTGA 2 cut(s) 79, 598
Hpy166II GTNNAC 2 cut(s) 749, 915
Hpy188I TCNGA 6 cut(s) 344, 463, 615, 639, 787, 936
Hpy188III TCNNGA 2 cut(s) 113, 833
Hpy8I GTNNAC 2 cut(s) 749, 915
HpyAV CCTTC 3 cut(s) 200, 349, 931
HpyCH4III ACNGT 4 cut(s) 97, 109, 782, 946
HpyCH4IV ACGT 1 cut(s) 751
HpyCH4V TGCA 4 cut(s) 82, 542, 713, 845
HpyF10VI GCNNNNNNNGC 4 cut(s) 15, 70, 79, 524
HpyF3I CTNAG 2 cut(s) 274, 519
HpySE526I ACGT 1 cut(s) 751
Hsp92II CATG 1 cut(s) 63
KflI GGGWCCC 1 cut(s) 115
Kzo9I GATC 5 cut(s) 124, 344, 463, 588, 810
LmnI GCTCC 2 cut(s) 38, 161
Lsp1109I GCAGC 3 cut(s) 410, 415, 514
LweI GCATC 3 cut(s) 50, 65, 266
MabI ACCWGGT 1 cut(s) 873
MaeI CTAG 3 cut(s) 632, 722, 744
MaeII ACGT 1 cut(s) 751
MaeIII GTNAC 2 cut(s) 31, 565
MalI GATC 5 cut(s) 126, 346, 465, 590, 812
MboI GATC 5 cut(s) 124, 344, 463, 588, 810
MboII GAAGA 4 cut(s) 215, 821, 964, 970
MfeI CAATTG 1 cut(s) 225
MflI RGATCY 2 cut(s) 463, 810
MluCI AATT 4 cut(s) 225, 302, 306, 555
MmeI TCCRAC 1 cut(s) 745
MnlI CCTC 7 cut(s) 80, 350, 400, 432, 514, 606, 781
MroXI GAANNNNTTC 4 cut(s) 174, 633, 663, 971
MseI TTAA 3 cut(s) 305, 333, 411
MspI CCGG 1 cut(s) 578
MspR9I CCNGG 2 cut(s) 579, 875
MunI CAATTG 1 cut(s) 225
MvaI CCWGG 1 cut(s) 875
MwoI GCNNNNNNNGC 4 cut(s) 15, 70, 79, 524
NciI CCSGG 1 cut(s) 579
NdeII GATC 5 cut(s) 124, 344, 463, 588, 810
NlaIII CATG 1 cut(s) 63
NlaIV GGNNCC 3 cut(s) 116, 117, 655
NmuCI GTSAC 1 cut(s) 31
PdmI GAANNNNTTC 4 cut(s) 174, 633, 663, 971
PfeI GAWTC 1 cut(s) 449
PflMI CCANNNNNTGG 1 cut(s) 901
PfoI TCCNGGA 1 cut(s) 577
PkrI GCNGC 4 cut(s) 8, 400, 430, 529
PpuMI RGGWCCY 1 cut(s) 115
PshBI ATTAAT 1 cut(s) 305
PsiI TTATAA 1 cut(s) 383
Psp5II RGGWCCY 1 cut(s) 115
Psp6I CCWGG 1 cut(s) 873
PspGI CCWGG 1 cut(s) 873
PspN4I GGNNCC 3 cut(s) 116, 117, 655
PspPI GGNCC 3 cut(s) 115, 593, 654
PspPPI RGGWCCY 1 cut(s) 115
PstNI CAGNNNCTG 1 cut(s) 467
PsuI RGATCY 2 cut(s) 463, 810
RsaI GTAC 1 cut(s) 806
RsaNI GTAC 1 cut(s) 805
SaqAI TTAA 3 cut(s) 305, 333, 411
SatI GCNGC 4 cut(s) 7, 399, 429, 528
Sau3AI GATC 5 cut(s) 124, 344, 463, 588, 810
Sau96I GGNCC 3 cut(s) 115, 593, 654
ScrFI CCNGG 2 cut(s) 579, 875
SexAI ACCWGGT 1 cut(s) 873
SfaNI GCATC 3 cut(s) 50, 65, 266
SinI GGWCC 2 cut(s) 115, 654
SmlI CTYRAG 1 cut(s) 619
SmoI CTYRAG 1 cut(s) 619
SpeI ACTAGT 1 cut(s) 631
Sse9I AATT 4 cut(s) 225, 302, 306, 555
SsiI CCGC 3 cut(s) 9, 293, 920
SspMI CTAG 3 cut(s) 632, 722, 744
StyD4I CCNGG 2 cut(s) 577, 873
StyI CCWWGG 1 cut(s) 882
TaaI ACNGT 4 cut(s) 97, 109, 782, 946
TaiI ACGT 1 cut(s) 754
TaqI TCGA 2 cut(s) 406, 444
TaqII GACCGA 1 cut(s) 671
TasI AATT 4 cut(s) 225, 302, 306, 555
TfiI GAWTC 1 cut(s) 449
Tru1I TTAA 3 cut(s) 305, 333, 411
Tru9I TTAA 3 cut(s) 305, 333, 411
TscAI CASTG 1 cut(s) 785
TseFI GTSAC 1 cut(s) 31
TseI GCWGC 4 cut(s) 6, 398, 428, 527
Tsp45I GTSAC 1 cut(s) 31
TspDTI ATGAA 3 cut(s) 40, 167, 462
TspRI CASTG 1 cut(s) 785
Van91I CCANNNNNTGG 1 cut(s) 901
VpaK11BI GGWCC 2 cut(s) 115, 654
VspI ATTAAT 1 cut(s) 305
XmiI GTMKAC 1 cut(s) 914
XmnI GAANNNNTTC 4 cut(s) 174, 633, 663, 971
XspI CTAG 3 cut(s) 632, 722, 744
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.