Rh1CG032900

Belongs to the CTL (choline transporter-like) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0002251
Physical Location & Seq
Reverse (-)
6323672 .. 6327624
3953 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG032900.1

Sequence Viewer

Length: 717 bp
ATGGGTGCCGCTGAACACGTAGTGGTGGAGGGAGAAAGTGAAGAGGTTGAGGCGAGGGAGAGAGATGAGAGGGAGGAGAAGGAAGAGGAGGAGGAGAAGAGAGAGCTGGAAGTGAAAGATGTGGAGAAAGTTGAGGTGTTGGGTTTTGAACAACACAACAATATTAATCATGATCAGAGTAGTCAGGGAGATGTTCATGTGTCAATGTTGCAGAGGCTTAATCCTACAAACCCTTTGAGAATTGCTATCAATGCTAAAACTCGTACTACTCCATCTCCATCTCTATCTGATTTCTCTCAGTCTCACACAACTCCTCCTCCTCAGCATTCTCAGCCTTGCTCTATTCCAACCCCTCAAAGAGCGTTGAGTAAAAATCTTGGAAGTGCTTGCCTTGGATCTCTGTTTGTGCCAACAATTGAAGCACTGAGAATTGTTGCTCGAGCTTTAAATCCACTTGAGGGAGAGGATGAGTTCATGTTTTCTTGTGCGCATTGCTGTCTCCATGTCATGCAGTGCATCTTCCGATATGGCAATGGCTGGGCCTTTGTTCAGATAGCAGCATATGGAAAAGATTTTGTGAGGGCACCACAAGAGACTTGGGAGCTCTTTGAAAGGCAAGGAATTCATCTCCTTGTTGATTCTGACATCACTAGCTCAATATGCTTCCTAACCGGCGTCTGCAGTGGCTCTATGTGCGTCATAGTTGTGGCTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Choline_transpo

Protein Analysis

238

Amino Acids

26.46

Weight (kDa)

4.93

Isoelectric Point (pI)

45.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Choline_transpo PF04515 119 - 235 5.4e-24 Plasma-membrane choline transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 489
AccB1I GGYRCC 2 cut(s) 5, 583
AciI CCGC 1 cut(s) 9
AclWI GGATC 1 cut(s) 403
AcsI RAATTY 1 cut(s) 621
AcyI GRCGYC 1 cut(s) 675
AdeI CACNNNGTG 1 cut(s) 22
AfaI GTAC 1 cut(s) 265
AfiI CCNNNNNNNGG 1 cut(s) 458
AflIII ACRYGT 1 cut(s) 16
AgsI TTSAA 3 cut(s) 149, 419, 611
AluBI AGCT 4 cut(s) 106, 443, 604, 654
AluI AGCT 4 cut(s) 106, 443, 604, 654
Alw21I GWGCWC 1 cut(s) 606
Alw26I GTCTC 3 cut(s) 306, 503, 587
AlwI GGATC 1 cut(s) 403
Ama87I CYCGRG 1 cut(s) 438
AoxI GGCC 1 cut(s) 540
ApeKI GCWGC 2 cut(s) 557, 710
ApoI RAATTY 1 cut(s) 621
AseI ATTAAT 1 cut(s) 165
AspLEI GCGC 1 cut(s) 490
AspS9I GGNCC 1 cut(s) 540
AvaI CYCGRG 1 cut(s) 438
BaeGI GKGCMC 1 cut(s) 586
BanI GGYRCC 2 cut(s) 5, 583
BanII GRGCYC 1 cut(s) 606
Bbv12I GWGCWC 1 cut(s) 606
BbvCI CCTCAGC 1 cut(s) 321
BbvI GCAGC 2 cut(s) 569, 697
BccI CCATC 2 cut(s) 280, 286
BclI TGATCA 1 cut(s) 172
BcoDI GTCTC 3 cut(s) 306, 503, 587
BfaI CTAG 1 cut(s) 651
BfmI CTRYAG 1 cut(s) 679
BisI GCNGC 3 cut(s) 9, 558, 711
BlsI GCNGC 3 cut(s) 10, 559, 712
BmeT110I CYCGRG 1 cut(s) 438
BmgT120I GGNCC 1 cut(s) 540
BmiI GGNNCC 2 cut(s) 7, 585
BmsI GCATC 1 cut(s) 525
Bpu10I CCTNAGC 1 cut(s) 321
BpuEI CTTGAG 1 cut(s) 476
BsaAI YACGTR 1 cut(s) 19
BsaHI GRCGYC 1 cut(s) 675
BsaJI CCNNGG 1 cut(s) 391
BsaXI ACNNNNNCTCC 6 cut(s) 259, 289, 298, 301, 328, 331
Bsc4I CCNNNNNNNGG 1 cut(s) 458
Bse118I RCCGGY 1 cut(s) 671
Bse3DI GCAATG 2 cut(s) 490, 538
BseDI CCNNGG 1 cut(s) 391
BseGI GGATG 1 cut(s) 472
BseLI CCNNNNNNNGG 1 cut(s) 458
BseMI GCAATG 2 cut(s) 490, 538
BseMII CTCAG 4 cut(s) 311, 335, 344, 416
BseRI GAGGAG 7 cut(s) 89, 101, 104, 107, 303, 306, 309
BseSI GKGCMC 1 cut(s) 586
BseXI GCAGC 2 cut(s) 569, 697
BseYI CCCAGC 1 cut(s) 537
BshFI GGCC 1 cut(s) 542
BshNI GGYRCC 2 cut(s) 5, 583
BsiHKAI GWGCWC 1 cut(s) 606
BsiHKCI CYCGRG 1 cut(s) 438
BsiSI CCGG 1 cut(s) 672
BslI CCNNNNNNNGG 1 cut(s) 458
BsmAI GTCTC 3 cut(s) 306, 503, 587
BsmI GAATGC 1 cut(s) 325
BsnI GGCC 1 cut(s) 542
BsoBI CYCGRG 1 cut(s) 438
Bsp1286I GDGCHC 2 cut(s) 586, 606
Bsp143I GATC 2 cut(s) 172, 395
BspACI CCGC 1 cut(s) 9
BspANI GGCC 1 cut(s) 542
BspCNI CTCAG 4 cut(s) 310, 334, 343, 417
BspHI TCATGA 1 cut(s) 169
BspLI GGNNCC 2 cut(s) 7, 585
BspMAI CTGCAG 1 cut(s) 683
BspPI GGATC 1 cut(s) 403
BspT107I GGYRCC 2 cut(s) 5, 583
BsrDI GCAATG 2 cut(s) 490, 538
BsrFI RCCGGY 1 cut(s) 671
BssAI RCCGGY 1 cut(s) 671
BssECI CCNNGG 1 cut(s) 391
BssMI GATC 2 cut(s) 172, 395
BssNI GRCGYC 1 cut(s) 675
BssT1I CCWWGG 1 cut(s) 391
Bst6I CTCTTC 3 cut(s) 36, 78, 92
BstACI GRCGYC 1 cut(s) 675
BstBAI YACGTR 1 cut(s) 19
BstC8I GCNNGC 1 cut(s) 388
BstDEI CTNAG 5 cut(s) 297, 321, 330, 425, 714
BstF5I GGATG 1 cut(s) 472
BstHHI GCGC 1 cut(s) 490
BstKTI GATC 2 cut(s) 175, 398
BstMAI GTCTC 3 cut(s) 306, 503, 587
BstMBI GATC 2 cut(s) 172, 395
BstMWI GCNNNNNNNGC 4 cut(s) 251, 331, 660, 693
BstSFI CTRYAG 1 cut(s) 679
BstSLI GKGCMC 1 cut(s) 586
BstV1I GCAGC 2 cut(s) 569, 697
BstX2I RGATCY 1 cut(s) 395
BstYI RGATCY 1 cut(s) 395
BsuRI GGCC 1 cut(s) 542
BtsCI GGATG 1 cut(s) 472
BtsI GCAGTG 2 cut(s) 518, 688
BtsIMutI CAGTG 3 cut(s) 422, 518, 688
Cac8I GCNNGC 1 cut(s) 388
CciI TCATGA 1 cut(s) 169
CfoI GCGC 1 cut(s) 490
Cfr10I RCCGGY 1 cut(s) 671
Cfr13I GGNCC 1 cut(s) 540
CseI GACGC 2 cut(s) 664, 685
Csp6I GTAC 1 cut(s) 264
CviAII CATG 5 cut(s) 170, 197, 475, 503, 508
CviQI GTAC 1 cut(s) 264
DdeI CTNAG 5 cut(s) 297, 321, 330, 425, 714
DpnI GATC 2 cut(s) 174, 397
DpnII GATC 2 cut(s) 172, 395
DraI TTTAAA 1 cut(s) 447
DraIII CACNNNGTG 1 cut(s) 22
Eam1104I CTCTTC 3 cut(s) 36, 78, 92
EarI CTCTTC 3 cut(s) 36, 78, 92
Ecl136II GAGCTC 1 cut(s) 604
Eco130I CCWWGG 1 cut(s) 391
Eco24I GRGCYC 1 cut(s) 606
Eco53kI GAGCTC 1 cut(s) 604
Eco88I CYCGRG 1 cut(s) 438
EcoICRI GAGCTC 1 cut(s) 604
EcoRI GAATTC 1 cut(s) 621
EcoT14I CCWWGG 1 cut(s) 391
EcoT38I GRGCYC 1 cut(s) 606
ErhI CCWWGG 1 cut(s) 391
FaeI CATG 5 cut(s) 173, 200, 478, 506, 511
FatI CATG 5 cut(s) 169, 196, 474, 502, 507
FauNDI CATATG 1 cut(s) 562
FbaI TGATCA 1 cut(s) 172
Fnu4HI GCNGC 3 cut(s) 9, 558, 711
FokI GGATG 1 cut(s) 479
FriOI GRGCYC 1 cut(s) 606
Fsp4HI GCNGC 3 cut(s) 9, 558, 711
FspAI RTGCGCAY 1 cut(s) 489
FspBI CTAG 1 cut(s) 651
FspI TGCGCA 1 cut(s) 489
GlaI GCGC 1 cut(s) 489
GluI GCNGC 3 cut(s) 9, 558, 711
GsaI CCCAGC 1 cut(s) 541
HaeIII GGCC 1 cut(s) 542
HapII CCGG 1 cut(s) 672
HgaI GACGC 2 cut(s) 664, 685
HhaI GCGC 1 cut(s) 490
Hin1I GRCGYC 1 cut(s) 675
Hin1II CATG 5 cut(s) 173, 200, 478, 506, 511
Hin6I GCGC 1 cut(s) 488
HinP1I GCGC 1 cut(s) 488
HinfI GANTC 1 cut(s) 638
HpaII CCGG 1 cut(s) 672
Hpy188I TCNGA 5 cut(s) 177, 289, 524, 552, 643
Hpy188III TCNNGA 1 cut(s) 170
HpyAV CCTTC 1 cut(s) 73
HpyCH4IV ACGT 1 cut(s) 18
HpyCH4V TGCA 4 cut(s) 211, 511, 516, 681
HpyF10VI GCNNNNNNNGC 4 cut(s) 251, 331, 660, 693
HpyF3I CTNAG 5 cut(s) 297, 321, 330, 425, 714
HpySE526I ACGT 1 cut(s) 18
Hsp92I GRCGYC 1 cut(s) 675
Hsp92II CATG 5 cut(s) 173, 200, 478, 506, 511
HspAI GCGC 1 cut(s) 488
Ksp22I TGATCA 1 cut(s) 172
Kzo9I GATC 2 cut(s) 172, 395
LmnI GCTCC 1 cut(s) 601
LpnPI CCDG 4 cut(s) 92, 170, 523, 685
Lsp1109I GCAGC 2 cut(s) 569, 697
LweI GCATC 1 cut(s) 525
MaeI CTAG 1 cut(s) 651
MaeII ACGT 1 cut(s) 18
MalI GATC 2 cut(s) 174, 397
MboI GATC 2 cut(s) 172, 395
MboII GAAGA 4 cut(s) 53, 95, 109, 511
MfeI CAATTG 1 cut(s) 414
MflI RGATCY 1 cut(s) 395
MhlI GDGCHC 2 cut(s) 586, 606
MluCI AATT 4 cut(s) 240, 414, 429, 621
MmeI TCCRAC 1 cut(s) 371
MseI TTAA 3 cut(s) 165, 219, 446
MslI CAYNNNNRTG 1 cut(s) 704
MspA1I CMGCKG 1 cut(s) 11
MspI CCGG 1 cut(s) 672
MunI CAATTG 1 cut(s) 414
Mva1269I GAATGC 1 cut(s) 325
MwoI GCNNNNNNNGC 4 cut(s) 251, 331, 660, 693
NdeI CATATG 1 cut(s) 562
NdeII GATC 2 cut(s) 172, 395
NlaIII CATG 5 cut(s) 173, 200, 478, 506, 511
NlaIV GGNNCC 2 cut(s) 7, 585
NsbI TGCGCA 1 cut(s) 489
PaeR7I CTCGAG 1 cut(s) 438
PagI TCATGA 1 cut(s) 169
PctI GAATGC 1 cut(s) 325
PfeI GAWTC 1 cut(s) 638
PkrI GCNGC 3 cut(s) 10, 559, 712
Ppu21I YACGTR 1 cut(s) 19
PshBI ATTAAT 1 cut(s) 165
Psp124BI GAGCTC 1 cut(s) 606
PspFI CCCAGC 1 cut(s) 537
PspN4I GGNNCC 2 cut(s) 7, 585
PspPI GGNCC 1 cut(s) 540
PspXI VCTCGAGB 1 cut(s) 438
PstI CTGCAG 1 cut(s) 683
PsuI RGATCY 1 cut(s) 395
RsaI GTAC 1 cut(s) 265
RsaNI GTAC 1 cut(s) 264
RseI CAYNNNNRTG 1 cut(s) 704
SacI GAGCTC 1 cut(s) 606
SaqAI TTAA 3 cut(s) 165, 219, 446
SatI GCNGC 3 cut(s) 9, 558, 711
Sau3AI GATC 2 cut(s) 172, 395
Sau96I GGNCC 1 cut(s) 540
SduI GDGCHC 2 cut(s) 586, 606
SetI ASST 7 cut(s) 21, 48, 108, 138, 445, 606, 656
SfaNI GCATC 1 cut(s) 525
SfcI CTRYAG 1 cut(s) 679
Sfr274I CTCGAG 1 cut(s) 438
SlaI CTCGAG 1 cut(s) 438
SmiMI CAYNNNNRTG 1 cut(s) 704
SmlI CTYRAG 2 cut(s) 438, 455
SmoI CTYRAG 2 cut(s) 438, 455
Sse9I AATT 4 cut(s) 240, 414, 429, 621
SsiI CCGC 1 cut(s) 9
SspI AATATT 1 cut(s) 163
SspMI CTAG 1 cut(s) 651
SstI GAGCTC 1 cut(s) 606
StyI CCWWGG 1 cut(s) 391
TaiI ACGT 1 cut(s) 21
TaqI TCGA 1 cut(s) 439
TasI AATT 4 cut(s) 240, 414, 429, 621
TauI GCSGC 1 cut(s) 11
TfiI GAWTC 1 cut(s) 638
Tru1I TTAA 3 cut(s) 165, 219, 446
Tru9I TTAA 3 cut(s) 165, 219, 446
TscAI CASTG 3 cut(s) 429, 518, 688
TseI GCWGC 2 cut(s) 557, 710
TspDTI ATGAA 3 cut(s) 185, 463, 614
TspRI CASTG 3 cut(s) 429, 518, 688
VspI ATTAAT 1 cut(s) 165
XapI RAATTY 1 cut(s) 621
XcmI CCANNNNNNNNNTGG 1 cut(s) 594
XhoI CTCGAG 1 cut(s) 438
XspI CTAG 1 cut(s) 651
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.