Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0001525

Physical Location & Seq

Genomic Coordinates Forward (+)

29477953 .. 29482337

Gene Structure 4385 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1210100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1686 bp

ATGCATCCCAGGTTCTATGATTTTGAAGCCACCTTTAAGGCCACCACCCAAAGGGGGTCAAACATGGGCACTTCATATTCTTCGTGCTATCATTCCACCACACTCTCAACTCACTCCCTCCTCCTCTCACACCCCAAAGCCTCTGCCTTTCATCCATCCAGATTCCCAAATCCCTGTTTCAAATTCCATTCCACAAACCAAATCCCCCAGACCCATCTGAGGATCATGTCCCAGAAACCCTCTCAGGTTTTGGGTGGGAAAGTTGAAGTTGGGGAGACCCAGGAGGGAGTTTCCAAAGGACCCATGATTGATTTTCATGGGATTGATGAGGGGCTGGTGGAGAAGATGGTGTATGATGCTCTTGTTTGGAGCTCTCTTCATGGGCTTCTTGTTGGTGACAAGTCTGTTCAGAGATCAGGAAAGGTACCTGGTGTAGGCATGGTACATGCACCGTTTGCTTTATTTCCGATGCTATTTCCAGAATGCCATTGGAAGCAAGCATATGAGTTAGCCCCCATATTTAATGAACTTGTCGATCGAGTGAGTTTGGATGCAAAGTTTCTGCAGGATTCACTGTCCAGAACGAAGAAAGTGGATGCTTTTACATCCAGACTTCTAGATATTCATTCCAAGATGCTAGAGATTAACAAGAAAGAGGACATACGCTTGGGTTTACATCGCTCAGATTATATGCTTGATGAAGAAACTAAACAACTCCTTCAAATAGAGATGAACACAATTTCAGCTTCATTTCCGGGACTTGGTTGTCTTGTCAGCGACCTTCACAGGAGTTTACTTAATCACTATGGAGAATCTCTTGGATTAGATTCCAAAAGAGTTCCAAATAATCCTGCAGCCACTAAGTTTGTAGAGGCTTTGGCTAAAGCTTGGAGTGAATACAATAACCCCAGGGCTGTAATCCTGCTCGTGGTTCAGCCTGAAGAGCGGAACATGTATGACCAACACTGGCTTTCTGCTCTAACGTTGGCCGAAATTGATGCAGAGGGGAAGCTTCAACCAGATGGAACACTCGTTGTGGGTAGCCATGAAGTTGCAATTGTTTATTTCAGAGTTGGGTATGCACCAATCAATTATCCTTCTGAATCAGAGTGGAGAGCAAGGCTTCTTATGGAGCAGTCATCTGCCATCAAGTGCCCCTCAATTTCTTATCACTTGGTCGGGACCAAAAAGATTCAACAAGAACTTGCAAAGCCTAATGTTCTTGAGAGGTTCCTTGATGATAAAGAAGACATTGCAAAATTGCGGAAATGCTTTGCAGGATTGTGGAGTTTGGATGACTCGGATATTGTCAAAAAGGCAATAGAGAGCCCTGGGTTATTTGTTATGAAGCCCCAAAGAGAAGGCGGAGGGAACAACATCTACGGCAATGATGTAAGGGAAGCCCTCACAAGATTGCAGAAGGAAGGATCAGAAGCAGATGCTGCTTATATCCTAATGCAGAGGATATTCCCTACTGCTTCTCACACATTCTTGATGCGCGAGGGTGTATGTTATAAAGATGATACCATATCTGAGCTGGGGGTATACGGTGCTTACTTAAGGAACAAAGATAAAGTGATCTTGAACGATCAATGCGGCTACCTGATGCGGACAAAGATTTCATCATCAGATGAAGGTGGAGTTGCAGCTGGATTTGCAGTCTTAGATAGTTTATATCTAACCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0000287 GO:0001101 GO:0003674 GO:0003824 GO:0004363 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006518 GO:0006520 GO:0006575 GO:0006749 GO:0006750 GO:0006790 GO:0006807 GO:0008144 GO:0008150 GO:0008152 GO:0009058 GO:0009507 GO:0009536 GO:0009719 GO:0009725 GO:0009753 GO:0009987 GO:0010033 GO:0016874 GO:0016879 GO:0016881 GO:0017076 GO:0019184 GO:0019752 GO:0030554 GO:0032553 GO:0032555 GO:0032559 GO:0033218 GO:0034641 GO:0035639 GO:0036094 GO:0042221 GO:0042277 GO:0042398 GO:0042802 GO:0042803 GO:0043043 GO:0043167 GO:0043168 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043295 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046872 GO:0046983 GO:0048037 GO:0050896 GO:0051186 GO:0051188 GO:0071704 GO:0072341 GO:0097159 GO:0097367 GO:1900750 GO:1901265 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1901681 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko00270 ko00480 ko01100 ko03008 ko03013 ko04216 map00270 map00480 map01100 map03008 map03013 map04216

Pfam Domains

Protein Families

GSH_synth_ATP

Protein Analysis

562

Amino Acids

62.79

Weight (kDa)

6.36

Isoelectric Point (pI)

43.04

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GSH_synth_ATP	PF03917	113 - 560	5.7e-129	Eukaryotic glutathione synthase, ATP binding domain
GSH_synthase	PF03199	305 - 393	8.1e-25	Eukaryotic glutathione synthase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001525)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G27380
fragaria_vesca	FvH4_6g05260 FvH4_6g05260 FvH4_6g05260
malus_domestica	MD04G1210100.v1.1 MD12G1224500.v1.1
prunus_persica	Prupe.6G331000_v2.0.a1 Prupe.6G331000_v2.0.a1 Prupe.6G331000_v2.0.a1
pyrus_communis	pycom04g18610 pycom04g18620 pycom12g21010
rosa_chinensis	RchiOBHm_Chr2g0172951 RchiOBHm_Chr2g0172961 RchiOBHm_Chr2g0172971 RchiOBHm_Chr2g0172981 RchiOBHm_Chr3g0453991 RchiOBHm_Chr6g0303351
rosa_laevigata	RLG00000022145 RLG00000022146 RLG00000025491
rosa_multiflora	Rmu_sc0003556.1_g000035 Rmu_sc0012743.1_g000009 Rmu_sc0019265.1_g000001
rosa_roxburghii	Rroxscaffold_1G00039730 Rroxscaffold_2G00079380 Rroxscaffold_6G00430810
rosa_rugosa	Rorug02G0563900 Rorug02G0563900 Rorug02G0564000 Rorug02G0564200 Rorug02G0652300
rosa_samantha	Rh1CG053200 Rh1DG057100 Rh2AG640700 Rh2AG640800 Rh2BG654200 Rh2BG654300 Rh2BG654500 Rh2CG619800 Rh2DG667800 Rh3AG054800 Rh3BG056400 Rh3CG055600 Rh3DG056900 Rh4AG280200 Rh4BG285600 Rh6AG435100 Rh6BG144800
rosa_wichuraiana	Rw2G052890 Rw3G004210

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1515
AasI	GACNNNNNNGTC	1 cut(s)	765
Acc65I	GGTACC	1 cut(s)	424
AccB1I	GGYRCC	1 cut(s)	424
AccBSI	CCGCTC	1 cut(s)	946
AccI	GTMKAC	1 cut(s)	1545
AccII	CGCG	1 cut(s)	1500
AciI	CCGC	5 cut(s)	946, 1264, 1365, 1596, 1609
AclI	AACGTT	1 cut(s)	983
AclWI	GGATC	2 cut(s)	230, 1435
AcoI	YGGCCR	1 cut(s)	987
AcsI	RAATTY	1 cut(s)	182
AcuI	CTGAAG	1 cut(s)	960
AfaI	GTAC	2 cut(s)	426, 444
AfiI	CCNNNNNNNGG	6 cut(s)	51, 54, 219, 434, 761, 928
AflII	CTTAAG	1 cut(s)	1558
AflIII	ACRYGT	1 cut(s)	951
AgsI	TTSAA	7 cut(s)	26, 181, 266, 722, 1016, 1196, 1585
AjnI	CCWGG	5 cut(s)	8, 279, 427, 908, 1330
AluBI	AGCT	6 cut(s)	372, 746, 887, 1012, 1537, 1649
AluI	AGCT	6 cut(s)	372, 746, 887, 1012, 1537, 1649
Alw21I	GWGCWC	1 cut(s)	374
Alw26I	GTCTC	1 cut(s)	269
AlwI	GGATC	2 cut(s)	230, 1435
AlwNI	CAGNNNCTG	1 cut(s)	1442
AoxI	GGCC	2 cut(s)	39, 987
ApeKI	GCWGC	3 cut(s)	854, 1442, 1646
ApoI	RAATTY	1 cut(s)	182
Asp718I	GGTACC	1 cut(s)	424
AspLEI	GCGC	1 cut(s)	1500
AspS9I	GGNCC	2 cut(s)	299, 1182
AsuC2I	CCSGG	1 cut(s)	756
AsuHPI	GGTGA	1 cut(s)	407
AvaII	GGWCC	2 cut(s)	299, 1182
BaeGI	GKGCMC	2 cut(s)	71, 1157
BanI	GGYRCC	1 cut(s)	424
BanII	GRGCYC	2 cut(s)	374, 1331
BauI	CACGAG	1 cut(s)	926
BbsI	GAAGAC	1 cut(s)	1254
Bbv12I	GWGCWC	1 cut(s)	374
BbvI	GCAGC	3 cut(s)	866, 1429, 1658
BccI	CCATC	5 cut(s)	163, 222, 340, 1016, 1154
BceAI	ACGGC	1 cut(s)	1399
BcgI	CGANNNNNNTGC	2 cut(s)	980, 1014
BciT130I	CCWGG	5 cut(s)	10, 281, 429, 910, 1332
BcnI	CCSGG	1 cut(s)	756
BcoDI	GTCTC	1 cut(s)	269
BfaI	CTAG	2 cut(s)	617, 638
BfmI	CTRYAG	2 cut(s)	563, 852
BfrI	CTTAAG	1 cut(s)	1558
BisI	GCNGC	4 cut(s)	855, 1443, 1597, 1647
BlsI	GCNGC	4 cut(s)	856, 1444, 1598, 1648
Bme1390I	CCNGG	6 cut(s)	10, 281, 429, 756, 910, 1332
Bme18I	GGWCC	2 cut(s)	299, 1182
BmgT120I	GGNCC	2 cut(s)	299, 1182
BmiI	GGNNCC	4 cut(s)	301, 426, 1183, 1232
BmrFI	CCNGG	6 cut(s)	10, 281, 429, 756, 910, 1332
BpiI	GAAGAC	1 cut(s)	1254
BpuEI	CTTGAG	1 cut(s)	1244
BpuMI	CCSGG	1 cut(s)	756
BsaI	GGTCTC	1 cut(s)	269
BsaJI	CCNNGG	6 cut(s)	8, 279, 908, 909, 1330, 1331
Bsc4I	CCNNNNNNNGG	6 cut(s)	51, 54, 219, 434, 761, 928
Bse1I	ACTGG	1 cut(s)	971
Bse3DI	GCAATG	2 cut(s)	1251, 1393
BseBI	CCWGG	5 cut(s)	10, 281, 429, 910, 1332
BseDI	CCNNGG	6 cut(s)	8, 279, 908, 909, 1330, 1331
BseGI	GGATG	7 cut(s)	4, 151, 155, 556, 601, 605, 1300
BseLI	CCNNNNNNNGG	6 cut(s)	51, 54, 219, 434, 761, 928
BseMI	GCAATG	2 cut(s)	1251, 1393
BseMII	CTCAG	4 cut(s)	209, 257, 696, 1524
BseNI	ACTGG	1 cut(s)	971
BseRI	GAGGAG	2 cut(s)	110, 113
BseSI	GKGCMC	2 cut(s)	71, 1157
BseXI	GCAGC	3 cut(s)	866, 1429, 1658
BseYI	CCCAGC	1 cut(s)	1537
Bsh1236I	CGCG	1 cut(s)	1500
Bsh1285I	CGRYCG	1 cut(s)	538
BshFI	GGCC	2 cut(s)	41, 989
BshNI	GGYRCC	1 cut(s)	424
BsiEI	CGRYCG	1 cut(s)	538
BsiHKAI	GWGCWC	1 cut(s)	374
BsiSI	CCGG	1 cut(s)	755
BslFI	GGGAC	3 cut(s)	214, 771, 1195
BslI	CCNNNNNNNGG	6 cut(s)	51, 54, 219, 434, 761, 928
BsmAI	GTCTC	1 cut(s)	269
BsmFI	GGGAC	3 cut(s)	214, 771, 1195
BsmI	GAATGC	1 cut(s)	488
BsnI	GGCC	2 cut(s)	41, 989
Bso31I	GGTCTC	1 cut(s)	269
Bsp1286I	GDGCHC	4 cut(s)	71, 374, 1157, 1331
Bsp143I	GATC	6 cut(s)	222, 413, 535, 1427, 1578, 1588
BspACI	CCGC	5 cut(s)	946, 1264, 1365, 1596, 1609
BspANI	GGCC	2 cut(s)	41, 989
BspCNI	CTCAG	4 cut(s)	210, 256, 695, 1525
BspFNI	CGCG	1 cut(s)	1500
BspLI	GGNNCC	4 cut(s)	301, 426, 1183, 1232
BspMAI	CTGCAG	2 cut(s)	567, 856
BspPI	GGATC	2 cut(s)	230, 1435
BspQI	GCTCTTC	1 cut(s)	936
BspT107I	GGYRCC	1 cut(s)	424
BspTI	CTTAAG	1 cut(s)	1558
BspTNI	GGTCTC	1 cut(s)	269
BsrBI	CCGCTC	1 cut(s)	946
BsrDI	GCAATG	2 cut(s)	1251, 1393
BsrI	ACTGG	1 cut(s)	971
BssECI	CCNNGG	6 cut(s)	8, 279, 908, 909, 1330, 1331
BssMI	GATC	6 cut(s)	222, 413, 535, 1427, 1578, 1588
BssNAI	GTATAC	1 cut(s)	1546
BssSI	CACGAG	1 cut(s)	926
Bst1107I	GTATAC	1 cut(s)	1546
Bst2BI	CACGAG	1 cut(s)	926
Bst2UI	CCWGG	5 cut(s)	10, 281, 429, 910, 1332
Bst4CI	ACNGT	3 cut(s)	453, 576, 1550
Bst6I	CTCTTC	2 cut(s)	381, 936
BstAFI	CTTAAG	1 cut(s)	1558
BstAPI	GCANNNNNTGC	2 cut(s)	455, 1442
BstC8I	GCNNGC	1 cut(s)	498
BstDEI	CTNAG	6 cut(s)	218, 243, 682, 861, 1533, 1663
BstENI	CCTNNNNNAGG	1 cut(s)	432
BstF5I	GGATG	7 cut(s)	4, 151, 155, 556, 601, 605, 1300
BstFNI	CGCG	1 cut(s)	1500
BstHHI	GCGC	1 cut(s)	1500
BstKTI	GATC	6 cut(s)	225, 416, 538, 1430, 1581, 1591
BstMAI	GTCTC	1 cut(s)	269
BstMBI	GATC	6 cut(s)	222, 413, 535, 1427, 1578, 1588
BstMCI	CGRYCG	1 cut(s)	538
BstMWI	GCNNNNNNNGC	4 cut(s)	455, 943, 1442, 1655
BstNI	CCWGG	5 cut(s)	10, 281, 429, 910, 1332
BstNSI	RCATGY	2 cut(s)	449, 955
BstSCI	CCNGG	6 cut(s)	8, 279, 427, 754, 908, 1330
BstSFI	CTRYAG	2 cut(s)	563, 852
BstSLI	GKGCMC	2 cut(s)	71, 1157
BstUI	CGCG	1 cut(s)	1500
BstV1I	GCAGC	3 cut(s)	866, 1429, 1658
BstV2I	GAAGAC	1 cut(s)	1254
BstZ17I	GTATAC	1 cut(s)	1546
BsuRI	GGCC	2 cut(s)	41, 989
BtgZI	GCGATG	1 cut(s)	662
BtsCI	GGATG	7 cut(s)	4, 151, 155, 556, 601, 605, 1300
BtsIMutI	CAGTG	2 cut(s)	572, 964
Cac8I	GCNNGC	1 cut(s)	498
CaiI	CAGNNNCTG	1 cut(s)	1442
CfoI	GCGC	1 cut(s)	1500
Cfr13I	GGNCC	2 cut(s)	299, 1182
CsiI	ACCWGGT	1 cut(s)	427
Csp6I	GTAC	2 cut(s)	425, 443
CspCI	CAANNNNNGTGG	2 cut(s)	847, 882
CviAII	CATG	9 cut(s)	64, 226, 304, 317, 380, 439, 446, 952, 1046
CviQI	GTAC	2 cut(s)	425, 443
DdeI	CTNAG	6 cut(s)	218, 243, 682, 861, 1533, 1663
DpnI	GATC	6 cut(s)	224, 415, 537, 1429, 1580, 1590
DpnII	GATC	6 cut(s)	222, 413, 535, 1427, 1578, 1588
DrdI	GACNNNNNNGTC	1 cut(s)	765
DseDI	GACNNNNNNGTC	1 cut(s)	765
EaeI	YGGCCR	1 cut(s)	987
Eam1104I	CTCTTC	2 cut(s)	381, 936
EarI	CTCTTC	2 cut(s)	381, 936
EciI	GGCGGA	1 cut(s)	1380
Ecl136II	GAGCTC	1 cut(s)	372
Eco24I	GRGCYC	2 cut(s)	374, 1331
Eco31I	GGTCTC	1 cut(s)	269
Eco47I	GGWCC	2 cut(s)	299, 1182
Eco53kI	GAGCTC	1 cut(s)	372
Eco57I	CTGAAG	1 cut(s)	960
EcoICRI	GAGCTC	1 cut(s)	372
EcoNI	CCTNNNNNAGG	1 cut(s)	432
EcoO109I	RGGNCCY	1 cut(s)	299
EcoRII	CCWGG	5 cut(s)	8, 279, 427, 908, 1330
EcoT22I	ATGCAT	1 cut(s)	6
EcoT38I	GRGCYC	2 cut(s)	374, 1331
FaeI	CATG	9 cut(s)	67, 229, 307, 320, 383, 442, 449, 955, 1049
FaqI	GGGAC	3 cut(s)	214, 771, 1195
FatI	CATG	9 cut(s)	63, 225, 303, 316, 379, 438, 445, 951, 1045
FauNDI	CATATG	1 cut(s)	502
FblI	GTMKAC	1 cut(s)	1545
Fnu4HI	GCNGC	4 cut(s)	855, 1443, 1597, 1647
FokI	GGATG	6 cut(s)	138, 142, 563, 592, 608, 1307
FriOI	GRGCYC	2 cut(s)	374, 1331
Fsp4HI	GCNGC	4 cut(s)	855, 1443, 1597, 1647
FspBI	CTAG	2 cut(s)	617, 638
GlaI	GCGC	1 cut(s)	1499
GluI	GCNGC	4 cut(s)	855, 1443, 1597, 1647
GsaI	CCCAGC	1 cut(s)	1541
HaeIII	GGCC	2 cut(s)	41, 989
HapII	CCGG	1 cut(s)	755
HhaI	GCGC	1 cut(s)	1500
Hin1II	CATG	9 cut(s)	67, 229, 307, 320, 383, 442, 449, 955, 1049
Hin6I	GCGC	1 cut(s)	1498
HinP1I	GCGC	1 cut(s)	1498
HindIII	AAGCTT	2 cut(s)	885, 1010
HinfI	GANTC	7 cut(s)	162, 569, 812, 827, 1103, 1192, 1298
HpaII	CCGG	1 cut(s)	755
HphI	GGTGA	1 cut(s)	407
Hpy166II	GTNNAC	3 cut(s)	674, 794, 1546
Hpy8I	GTNNAC	3 cut(s)	674, 794, 1546
HpyAV	CCTTC	7 cut(s)	728, 791, 1107, 1355, 1414, 1418, 1628
HpyCH4III	ACNGT	3 cut(s)	453, 576, 1550
HpyCH4IV	ACGT	1 cut(s)	983
HpyF10VI	GCNNNNNNNGC	4 cut(s)	455, 943, 1442, 1655
HpyF3I	CTNAG	6 cut(s)	218, 243, 682, 861, 1533, 1663
HpySE526I	ACGT	1 cut(s)	983
Hsp92II	CATG	9 cut(s)	67, 229, 307, 320, 383, 442, 449, 955, 1049
HspAI	GCGC	1 cut(s)	1498
KpnI	GGTACC	1 cut(s)	428
Kzo9I	GATC	6 cut(s)	222, 413, 535, 1427, 1578, 1588
LguI	GCTCTTC	1 cut(s)	936
LmnI	GCTCC	2 cut(s)	369, 1132
Lsp1109I	GCAGC	3 cut(s)	866, 1429, 1658
MabI	ACCWGGT	1 cut(s)	427
MaeI	CTAG	2 cut(s)	617, 638
MaeII	ACGT	1 cut(s)	983
MaeIII	GTNAC	1 cut(s)	395
MalI	GATC	6 cut(s)	224, 415, 537, 1429, 1580, 1590
MbiI	CCGCTC	1 cut(s)	946
MboI	GATC	6 cut(s)	222, 413, 535, 1427, 1578, 1588
MboII	GAAGA	7 cut(s)	72, 355, 368, 598, 713, 953, 1259
MfeI	CAATTG	1 cut(s)	1056
MhlI	GDGCHC	4 cut(s)	71, 374, 1157, 1331
MluCI	AATT	7 cut(s)	182, 738, 993, 1056, 1090, 1161, 1259
MlyI	GAGTC	1 cut(s)	1292
Mph1103I	ATGCAT	1 cut(s)	6
MseI	TTAA	5 cut(s)	36, 522, 645, 798, 1559
MspA1I	CMGCKG	1 cut(s)	1649
MspCI	CTTAAG	1 cut(s)	1558
MspI	CCGG	1 cut(s)	755
MspR9I	CCNGG	6 cut(s)	10, 281, 429, 756, 910, 1332
MunI	CAATTG	1 cut(s)	1056
Mva1269I	GAATGC	1 cut(s)	488
MvaI	CCWGG	5 cut(s)	10, 281, 429, 910, 1332
MvnI	CGCG	1 cut(s)	1500
MwoI	GCNNNNNNNGC	4 cut(s)	455, 943, 1442, 1655
NciI	CCSGG	1 cut(s)	756
NdeI	CATATG	1 cut(s)	502
NdeII	GATC	6 cut(s)	222, 413, 535, 1427, 1578, 1588
NlaIII	CATG	9 cut(s)	67, 229, 307, 320, 383, 442, 449, 955, 1049
NlaIV	GGNNCC	4 cut(s)	301, 426, 1183, 1232
NmuCI	GTSAC	1 cut(s)	395
NsiI	ATGCAT	1 cut(s)	6
NspI	RCATGY	2 cut(s)	449, 955
PasI	CCCWGGG	2 cut(s)	909, 1331
PciI	ACATGT	1 cut(s)	951
PciSI	GCTCTTC	1 cut(s)	936
PctI	GAATGC	1 cut(s)	488
PfeI	GAWTC	6 cut(s)	162, 569, 812, 827, 1103, 1192
PfoI	TCCNGGA	1 cut(s)	754
PkrI	GCNGC	4 cut(s)	856, 1444, 1598, 1648
Ple19I	CGATCG	1 cut(s)	538
PleI	GAGTC	1 cut(s)	1292
PpsI	GAGTC	1 cut(s)	1292
PpuMI	RGGWCCY	1 cut(s)	299
PscI	ACATGT	1 cut(s)	951
PsiI	TTATAA	1 cut(s)	1515
Psp124BI	GAGCTC	1 cut(s)	374
Psp1406I	AACGTT	1 cut(s)	983
Psp5II	RGGWCCY	1 cut(s)	299
Psp6I	CCWGG	5 cut(s)	8, 279, 427, 908, 1330
PspFI	CCCAGC	1 cut(s)	1537
PspGI	CCWGG	5 cut(s)	8, 279, 427, 908, 1330
PspN4I	GGNNCC	4 cut(s)	301, 426, 1183, 1232
PspPI	GGNCC	2 cut(s)	299, 1182
PspPPI	RGGWCCY	1 cut(s)	299
PsrI	GAACNNNNNNTAC	2 cut(s)	1364, 1396
PstI	CTGCAG	2 cut(s)	567, 856
PstNI	CAGNNNCTG	1 cut(s)	1442
PvuI	CGATCG	1 cut(s)	538
PvuII	CAGCTG	1 cut(s)	1649
RsaI	GTAC	2 cut(s)	426, 444
RsaNI	GTAC	2 cut(s)	425, 443
SacI	GAGCTC	1 cut(s)	374
SapI	GCTCTTC	1 cut(s)	936
SaqAI	TTAA	5 cut(s)	36, 522, 645, 798, 1559
SatI	GCNGC	4 cut(s)	855, 1443, 1597, 1647
Sau3AI	GATC	6 cut(s)	222, 413, 535, 1427, 1578, 1588
Sau96I	GGNCC	2 cut(s)	299, 1182
SchI	GAGTC	1 cut(s)	1292
ScrFI	CCNGG	6 cut(s)	10, 281, 429, 756, 910, 1332
SduI	GDGCHC	4 cut(s)	71, 374, 1157, 1331
SexAI	ACCWGGT	1 cut(s)	427
SfcI	CTRYAG	2 cut(s)	563, 852
SinI	GGWCC	2 cut(s)	299, 1182
SmlI	CTYRAG	2 cut(s)	1223, 1558
SmoI	CTYRAG	2 cut(s)	1223, 1558
Sse9I	AATT	7 cut(s)	182, 738, 993, 1056, 1090, 1161, 1259
SsiI	CCGC	5 cut(s)	946, 1264, 1365, 1596, 1609
SspMI	CTAG	2 cut(s)	617, 638
SstI	GAGCTC	1 cut(s)	374
StyD4I	CCNGG	6 cut(s)	8, 279, 427, 754, 908, 1330
TaaI	ACNGT	3 cut(s)	453, 576, 1550
TaiI	ACGT	1 cut(s)	986
TaqI	TCGA	2 cut(s)	534, 538
TasI	AATT	7 cut(s)	182, 738, 993, 1056, 1090, 1161, 1259
TauI	GCSGC	1 cut(s)	1599
TfiI	GAWTC	6 cut(s)	162, 569, 812, 827, 1103, 1192
Tru1I	TTAA	5 cut(s)	36, 522, 645, 798, 1559
Tru9I	TTAA	5 cut(s)	36, 522, 645, 798, 1559
TscAI	CASTG	2 cut(s)	579, 971
TseFI	GTSAC	1 cut(s)	395
TseI	GCWGC	3 cut(s)	854, 1442, 1646
Tsp45I	GTSAC	1 cut(s)	395
TspRI	CASTG	2 cut(s)	579, 971
Vha464I	CTTAAG	1 cut(s)	1558
VpaK11BI	GGWCC	2 cut(s)	299, 1182
XagI	CCTNNNNNAGG	1 cut(s)	432
XapI	RAATTY	1 cut(s)	182
XbaI	TCTAGA	1 cut(s)	616
XceI	RCATGY	2 cut(s)	449, 955
XcmI	CCANNNNNNNNNTGG	2 cut(s)	486, 1534
XmiI	GTMKAC	1 cut(s)	1545
XspI	CTAG	2 cut(s)	617, 638
Zsp2I	ATGCAT	1 cut(s)	6

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.