Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0001525

Physical Location & Seq

Genomic Coordinates Forward (+)

30065235 .. 30069626

Gene Structure 4392 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1224500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1614 bp

ATGAGTTTAAAGCCACTTTTAAGGCCACCACCCAAATGGTCAAACATGGGCACTTCATATTCTTCGTGCTCTCATTCCACCACCACACTCTCCACTCACTCCCTCCTCCTCACGCACCCCAAATCATCTGCCTTTAAGCAATCAGGATTCCCAAATCCCGATTCCAAATTCCATTCCACCCACCAAATTCCGCAGACCCATCTGAGGATCATGTCCCAGAAACCCTCTCAGGTTTTGGGTGGGAAAGTTGAAGTTGGGGAGACCCAGGAGGGAATTTCCAAAAGACCCATGATTGATTTTCATGGGATTGATGAGGGGCTGGTGGAGAAGATGGTGTATGATGCTCTTGTTTGGAGCTCTCTTCATGGGCTTGTTGTTGGTGACAAGTCCGTTCAGAGATCAGGAAAAGTACCTGGTGTAGGCATGGTACATGCACCATTTGCTTTGTTTCCGATGCCGTTTCCAGAAAGTCATTGGAAGCAAGCATATGAGTTATCCCCCATATTTAATGAACTTGTCGATCGAGTGAGTTTGGATGCAAAGTTTCTGCAGGATTCACTGTCCAGAACGAAGAAAGTGGATGCTTTTACATCTAGACTTCTAGATATTCACTCCAAGATGCTAGAGATTAACAAGAAAGAGGATATACGCTTGGGTTTACATCGCTCAGATTATATGCTTGATGAAGAAACTAAACAACTCCTTCAAATAGAGATGAACACAATTTCATCTTCATTTCCGGGACTCGGTTGTCTTGTCAGCGACCTTCACAGGAGCTTACTTAATCACTATGGAGAATATCTTGGATTAGATTCCAGAAGAGTTCCTAATAATCCTGCAGCCACTCAGTTTGTAGAGGCTTTGGCTAAAGCTTGGACTGAATACAATAACCCCAGGTCCGTAATCCTGTTCGTGGTTCAGCCTGAAGAGCGGAACATGTATGACCAACACTGGCTTTCTGCTCTGGGTGGCCAAGAAGTTGCAATTGTTTATTTCAGAGCTGGGTATACACCAATTGATTATCCATCTGAATCAGAGTGGAGAGCAAGGTTCCTTATGGAGCAGTCATCTGCCATCAAGTGCCCCTCAATTTCTTATCATCTAGTTGGGACCAAAAAGATTCAACAAGAACTTGCAAAGCCTAACGTTCTTGAGAGGTTCCTTAATGATAAAGAGGACATTGCAAAACTGCGAAAATGCTTTGCAGGATTGTGGAGTTTGGATGACTCGGATATTGTCAGAAAGGCAATAGAGATCCCCGGGTTATTTGTTATGAAGCCCCAAAGAGAAGGCGGAGGGAACAATATCTATGGTGATGACGTAAGGGAAGCCCTTGTACGATTGCAGAAGGAAGGGTCAGAAGCAGATGCTGCTTATATCCTAATGCAGAGGATATTCCCAACTGTTTCTCACACATTCTTGATGCGTGAGGGCATATGTTATAAAGATGATACCATATCTGAGCTCGGGGTATACGGTGCTTACTTAAGGAACAAGGATAAAGTGATCTTGAACGACCAATGCGGCTACCTGATGCGGACAAAGGTTTCCTCATCAGATGAAGGTGGAGTTGCAGCTGGATTTGCAGTCTTAGATAGTTTATATCTAACCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0000287 GO:0001101 GO:0003674 GO:0003824 GO:0004363 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006518 GO:0006520 GO:0006575 GO:0006749 GO:0006750 GO:0006790 GO:0006807 GO:0008144 GO:0008150 GO:0008152 GO:0009058 GO:0009507 GO:0009536 GO:0009719 GO:0009725 GO:0009753 GO:0009987 GO:0010033 GO:0016874 GO:0016879 GO:0016881 GO:0017076 GO:0019184 GO:0019752 GO:0030554 GO:0032553 GO:0032555 GO:0032559 GO:0033218 GO:0034641 GO:0035639 GO:0036094 GO:0042221 GO:0042277 GO:0042398 GO:0042802 GO:0042803 GO:0043043 GO:0043167 GO:0043168 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043295 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046872 GO:0046983 GO:0048037 GO:0050896 GO:0051186 GO:0051188 GO:0071704 GO:0072341 GO:0097159 GO:0097367 GO:1900750 GO:1901265 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1901681 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko00270 ko00480 ko01100 ko03008 ko03013 ko04216 map00270 map00480 map01100 map03008 map03013 map04216

Pfam Domains

Protein Families

GSH_synth_ATP

Protein Analysis

538

Amino Acids

60.4

Weight (kDa)

6.64

Isoelectric Point (pI)

47.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GSH_synth_ATP	PF03917	108 - 536	2.7e-129	Eukaryotic glutathione synthase, ATP binding domain
GSH_synthase	PF03199	325 - 369	1.3e-18	Eukaryotic glutathione synthase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001525)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G27380
fragaria_vesca	FvH4_6g05260 FvH4_6g05260 FvH4_6g05260
malus_domestica	MD04G1210100.v1.1 MD12G1224500.v1.1
prunus_persica	Prupe.6G331000_v2.0.a1 Prupe.6G331000_v2.0.a1 Prupe.6G331000_v2.0.a1
pyrus_communis	pycom04g18610 pycom04g18620 pycom12g21010
rosa_chinensis	RchiOBHm_Chr2g0172951 RchiOBHm_Chr2g0172961 RchiOBHm_Chr2g0172971 RchiOBHm_Chr2g0172981 RchiOBHm_Chr3g0453991 RchiOBHm_Chr6g0303351
rosa_laevigata	RLG00000022145 RLG00000022146 RLG00000025491
rosa_multiflora	Rmu_sc0003556.1_g000035 Rmu_sc0012743.1_g000009 Rmu_sc0019265.1_g000001
rosa_roxburghii	Rroxscaffold_1G00039730 Rroxscaffold_2G00079380 Rroxscaffold_6G00430810
rosa_rugosa	Rorug02G0563900 Rorug02G0563900 Rorug02G0564000 Rorug02G0564200 Rorug02G0652300
rosa_samantha	Rh1CG053200 Rh1DG057100 Rh2AG640700 Rh2AG640800 Rh2BG654200 Rh2BG654300 Rh2BG654500 Rh2CG619800 Rh2DG667800 Rh3AG054800 Rh3BG056400 Rh3CG055600 Rh3DG056900 Rh4AG280200 Rh4BG285600 Rh6AG435100 Rh6BG144800
rosa_wichuraiana	Rw2G052890 Rw3G004210

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1443
AasI	GACNNNNNNGTC	1 cut(s)	750
AccBSI	CCGCTC	1 cut(s)	931
AccI	GTMKAC	2 cut(s)	1007, 1473
AciI	CCGC	5 cut(s)	191, 931, 1293, 1524, 1537
AclI	AACGTT	1 cut(s)	1146
AclWI	GGATC	2 cut(s)	215, 1249
AcoI	YGGCCR	1 cut(s)	970
AcsI	RAATTY	3 cut(s)	167, 186, 273
AcuI	CTGAAG	1 cut(s)	945
AfaI	GTAC	3 cut(s)	411, 429, 1338
AfiI	CCNNNNNNNGG	4 cut(s)	204, 419, 746, 913
AflII	CTTAAG	1 cut(s)	1486
AflIII	ACRYGT	1 cut(s)	936
AgsI	TTSAA	4 cut(s)	251, 707, 1124, 1513
AjnI	CCWGG	3 cut(s)	264, 412, 893
AjuI	GAANNNNNNNTTGG	2 cut(s)	145, 177
AluBI	AGCT	6 cut(s)	357, 777, 872, 1001, 1465, 1577
AluI	AGCT	6 cut(s)	357, 777, 872, 1001, 1465, 1577
Alw21I	GWGCWC	3 cut(s)	71, 359, 1467
Alw26I	GTCTC	1 cut(s)	254
AlwI	GGATC	2 cut(s)	215, 1249
AlwNI	CAGNNNCTG	1 cut(s)	1370
Ama87I	CYCGRG	2 cut(s)	1259, 1466
AoxI	GGCC	2 cut(s)	23, 970
ApeKI	GCWGC	3 cut(s)	839, 1370, 1574
ApoI	RAATTY	3 cut(s)	167, 186, 273
AspS9I	GGNCC	2 cut(s)	897, 1110
AsuC2I	CCSGG	3 cut(s)	741, 1260, 1261
AsuHPI	GGTGA	2 cut(s)	392, 1325
AvaI	CYCGRG	2 cut(s)	1259, 1466
AvaII	GGWCC	2 cut(s)	897, 1110
BaeGI	GKGCMC	2 cut(s)	53, 1085
BalI	TGGCCA	1 cut(s)	972
BanII	GRGCYC	2 cut(s)	359, 1467
BarI	GAAGNNNNNNTAC	2 cut(s)	1320, 1352
Bbv12I	GWGCWC	3 cut(s)	71, 359, 1467
BbvI	GCAGC	3 cut(s)	851, 1357, 1586
BccI	CCATC	4 cut(s)	207, 325, 1033, 1082
BceAI	ACGGC	1 cut(s)	442
BciT130I	CCWGG	3 cut(s)	266, 414, 895
BcnI	CCSGG	3 cut(s)	741, 1260, 1261
BcoDI	GTCTC	1 cut(s)	254
BfaI	CTAG	4 cut(s)	594, 602, 623, 1103
BfmI	CTRYAG	2 cut(s)	548, 837
BfrI	CTTAAG	1 cut(s)	1486
BisI	GCNGC	4 cut(s)	840, 1371, 1525, 1575
BlsI	GCNGC	4 cut(s)	841, 1372, 1526, 1576
Bme1390I	CCNGG	6 cut(s)	266, 414, 741, 895, 1260, 1261
Bme18I	GGWCC	2 cut(s)	897, 1110
BmeT110I	CYCGRG	2 cut(s)	1259, 1466
BmgT120I	GGNCC	2 cut(s)	897, 1110
BmiI	GGNNCC	3 cut(s)	1052, 1111, 1160
BmrFI	CCNGG	6 cut(s)	266, 414, 741, 895, 1260, 1261
BmsI	GCATC	8 cut(s)	331, 444, 526, 571, 609, 1357, 1413, 1524
BpuEI	CTTGAG	1 cut(s)	1172
BpuMI	CCSGG	3 cut(s)	741, 1260, 1261
BsaI	GGTCTC	1 cut(s)	254
BsaJI	CCNNGG	4 cut(s)	264, 893, 1258, 1259
Bsc4I	CCNNNNNNNGG	4 cut(s)	204, 419, 746, 913
Bse1I	ACTGG	1 cut(s)	956
Bse3DI	GCAATG	1 cut(s)	1179
BseBI	CCWGG	3 cut(s)	266, 414, 895
BseDI	CCNNGG	4 cut(s)	264, 893, 1258, 1259
BseGI	GGATG	3 cut(s)	541, 586, 1228
BseLI	CCNNNNNNNGG	4 cut(s)	204, 419, 746, 913
BseMI	GCAATG	1 cut(s)	1179
BseMII	CTCAG	5 cut(s)	194, 242, 681, 860, 1452
BseNI	ACTGG	1 cut(s)	956
BseRI	GAGGAG	2 cut(s)	95, 98
BseSI	GKGCMC	2 cut(s)	53, 1085
BseXI	GCAGC	3 cut(s)	851, 1357, 1586
BseYI	CCCAGC	1 cut(s)	1001
Bsh1285I	CGRYCG	1 cut(s)	523
BshFI	GGCC	2 cut(s)	25, 972
BsiEI	CGRYCG	1 cut(s)	523
BsiHKAI	GWGCWC	3 cut(s)	71, 359, 1467
BsiHKCI	CYCGRG	2 cut(s)	1259, 1466
BsiSI	CCGG	2 cut(s)	740, 1260
BslFI	GGGAC	3 cut(s)	199, 756, 1123
BslI	CCNNNNNNNGG	4 cut(s)	204, 419, 746, 913
BsmAI	GTCTC	1 cut(s)	254
BsmFI	GGGAC	3 cut(s)	199, 756, 1123
BsnI	GGCC	2 cut(s)	25, 972
Bso31I	GGTCTC	1 cut(s)	254
BsoBI	CYCGRG	2 cut(s)	1259, 1466
Bsp1286I	GDGCHC	5 cut(s)	53, 71, 359, 1085, 1467
Bsp143I	GATC	5 cut(s)	207, 398, 520, 1254, 1506
BspACI	CCGC	5 cut(s)	191, 931, 1293, 1524, 1537
BspANI	GGCC	2 cut(s)	25, 972
BspCNI	CTCAG	5 cut(s)	195, 241, 680, 859, 1453
BspLI	GGNNCC	3 cut(s)	1052, 1111, 1160
BspMAI	CTGCAG	2 cut(s)	552, 841
BspPI	GGATC	2 cut(s)	215, 1249
BspQI	GCTCTTC	1 cut(s)	921
BspTI	CTTAAG	1 cut(s)	1486
BspTNI	GGTCTC	1 cut(s)	254
BsrBI	CCGCTC	1 cut(s)	931
BsrDI	GCAATG	1 cut(s)	1179
BsrI	ACTGG	1 cut(s)	956
BssECI	CCNNGG	4 cut(s)	264, 893, 1258, 1259
BssMI	GATC	5 cut(s)	207, 398, 520, 1254, 1506
BssNAI	GTATAC	2 cut(s)	1008, 1474
Bst1107I	GTATAC	2 cut(s)	1008, 1474
Bst2UI	CCWGG	3 cut(s)	266, 414, 895
Bst4CI	ACNGT	3 cut(s)	561, 1405, 1478
Bst6I	CTCTTC	3 cut(s)	366, 814, 921
BstAFI	CTTAAG	1 cut(s)	1486
BstAPI	GCANNNNNTGC	2 cut(s)	440, 1370
BstC8I	GCNNGC	1 cut(s)	483
BstDEI	CTNAG	6 cut(s)	203, 228, 667, 846, 1461, 1591
BstENI	CCTNNNNNAGG	1 cut(s)	417
BstF5I	GGATG	3 cut(s)	541, 586, 1228
BstKTI	GATC	5 cut(s)	210, 401, 523, 1257, 1509
BstMAI	GTCTC	1 cut(s)	254
BstMBI	GATC	5 cut(s)	207, 398, 520, 1254, 1506
BstMCI	CGRYCG	1 cut(s)	523
BstMWI	GCNNNNNNNGC	4 cut(s)	440, 928, 1370, 1583
BstNI	CCWGG	3 cut(s)	266, 414, 895
BstNSI	RCATGY	2 cut(s)	434, 940
BstSCI	CCNGG	6 cut(s)	264, 412, 739, 893, 1258, 1259
BstSFI	CTRYAG	2 cut(s)	548, 837
BstSLI	GKGCMC	2 cut(s)	53, 1085
BstV1I	GCAGC	3 cut(s)	851, 1357, 1586
BstX2I	RGATCY	1 cut(s)	1254
BstXI	CCANNNNNNTGG	1 cut(s)	36
BstYI	RGATCY	1 cut(s)	1254
BstZ17I	GTATAC	2 cut(s)	1008, 1474
BsuRI	GGCC	2 cut(s)	25, 972
BtgZI	GCGATG	1 cut(s)	647
BtsCI	GGATG	3 cut(s)	541, 586, 1228
BtsIMutI	CAGTG	2 cut(s)	557, 949
Cac8I	GCNNGC	1 cut(s)	483
CaiI	CAGNNNCTG	1 cut(s)	1370
Cfr13I	GGNCC	2 cut(s)	897, 1110
Cfr9I	CCCGGG	1 cut(s)	1259
CsiI	ACCWGGT	1 cut(s)	412
Csp6I	GTAC	3 cut(s)	410, 428, 1337
CspCI	CAANNNNNGTGG	2 cut(s)	832, 867
CviAII	CATG	8 cut(s)	46, 211, 289, 302, 365, 424, 431, 937
CviQI	GTAC	3 cut(s)	410, 428, 1337
DdeI	CTNAG	6 cut(s)	203, 228, 667, 846, 1461, 1591
DpnI	GATC	5 cut(s)	209, 400, 522, 1256, 1508
DpnII	GATC	5 cut(s)	207, 398, 520, 1254, 1506
DraI	TTTAAA	1 cut(s)	9
DrdI	GACNNNNNNGTC	1 cut(s)	750
DseDI	GACNNNNNNGTC	1 cut(s)	750
EaeI	YGGCCR	1 cut(s)	970
Eam1104I	CTCTTC	3 cut(s)	366, 814, 921
EarI	CTCTTC	3 cut(s)	366, 814, 921
EciI	GGCGGA	1 cut(s)	1308
Ecl136II	GAGCTC	2 cut(s)	357, 1465
Eco24I	GRGCYC	2 cut(s)	359, 1467
Eco31I	GGTCTC	1 cut(s)	254
Eco47I	GGWCC	2 cut(s)	897, 1110
Eco53kI	GAGCTC	2 cut(s)	357, 1465
Eco57I	CTGAAG	1 cut(s)	945
Eco88I	CYCGRG	2 cut(s)	1259, 1466
EcoICRI	GAGCTC	2 cut(s)	357, 1465
EcoNI	CCTNNNNNAGG	1 cut(s)	417
EcoRII	CCWGG	3 cut(s)	264, 412, 893
EcoT38I	GRGCYC	2 cut(s)	359, 1467
FaeI	CATG	8 cut(s)	49, 214, 292, 305, 368, 427, 434, 940
FaqI	GGGAC	3 cut(s)	199, 756, 1123
FatI	CATG	8 cut(s)	45, 210, 288, 301, 364, 423, 430, 936
FauNDI	CATATG	2 cut(s)	487, 1436
FblI	GTMKAC	2 cut(s)	1007, 1473
Fnu4HI	GCNGC	4 cut(s)	840, 1371, 1525, 1575
FokI	GGATG	3 cut(s)	548, 593, 1235
FriOI	GRGCYC	2 cut(s)	359, 1467
Fsp4HI	GCNGC	4 cut(s)	840, 1371, 1525, 1575
FspBI	CTAG	4 cut(s)	594, 602, 623, 1103
GluI	GCNGC	4 cut(s)	840, 1371, 1525, 1575
GsaI	CCCAGC	1 cut(s)	1005
HaeIII	GGCC	2 cut(s)	25, 972
HapII	CCGG	2 cut(s)	740, 1260
Hin1II	CATG	8 cut(s)	49, 214, 292, 305, 368, 427, 434, 940
HindIII	AAGCTT	1 cut(s)	870
HinfI	GANTC	8 cut(s)	147, 161, 554, 744, 812, 1031, 1120, 1226
HpaII	CCGG	2 cut(s)	740, 1260
HphI	GGTGA	2 cut(s)	392, 1325
Hpy166II	GTNNAC	3 cut(s)	659, 1008, 1474
Hpy8I	GTNNAC	3 cut(s)	659, 1008, 1474
HpyAV	CCTTC	6 cut(s)	713, 776, 1283, 1342, 1346, 1556
HpyCH4III	ACNGT	3 cut(s)	561, 1405, 1478
HpyCH4IV	ACGT	2 cut(s)	1146, 1320
HpyF10VI	GCNNNNNNNGC	4 cut(s)	440, 928, 1370, 1583
HpyF3I	CTNAG	6 cut(s)	203, 228, 667, 846, 1461, 1591
HpySE526I	ACGT	2 cut(s)	1146, 1320
Hsp92II	CATG	8 cut(s)	49, 214, 292, 305, 368, 427, 434, 940
Kzo9I	GATC	5 cut(s)	207, 398, 520, 1254, 1506
LguI	GCTCTTC	1 cut(s)	921
LmnI	GCTCC	3 cut(s)	354, 774, 1060
Lsp1109I	GCAGC	3 cut(s)	851, 1357, 1586
LweI	GCATC	8 cut(s)	331, 444, 526, 571, 609, 1357, 1413, 1524
MabI	ACCWGGT	1 cut(s)	412
MaeI	CTAG	4 cut(s)	594, 602, 623, 1103
MaeII	ACGT	2 cut(s)	1146, 1320
MaeIII	GTNAC	1 cut(s)	380
MalI	GATC	5 cut(s)	209, 400, 522, 1256, 1508
MbiI	CCGCTC	1 cut(s)	931
MboI	GATC	5 cut(s)	207, 398, 520, 1254, 1506
MboII	GAAGA	8 cut(s)	54, 340, 353, 583, 698, 723, 831, 938
MfeI	CAATTG	2 cut(s)	984, 1014
MflI	RGATCY	1 cut(s)	1254
MhlI	GDGCHC	5 cut(s)	53, 71, 359, 1085, 1467
MlsI	TGGCCA	1 cut(s)	972
MluCI	AATT	7 cut(s)	167, 186, 273, 723, 984, 1014, 1089
MluNI	TGGCCA	1 cut(s)	972
MlyI	GAGTC	2 cut(s)	738, 1220
Mox20I	TGGCCA	1 cut(s)	972
MscI	TGGCCA	1 cut(s)	972
MseI	TTAA	8 cut(s)	8, 20, 135, 507, 630, 783, 1164, 1487
MslI	CAYNNNNRTG	1 cut(s)	34
Msp20I	TGGCCA	1 cut(s)	972
MspA1I	CMGCKG	1 cut(s)	1577
MspCI	CTTAAG	1 cut(s)	1486
MspI	CCGG	2 cut(s)	740, 1260
MspR9I	CCNGG	6 cut(s)	266, 414, 741, 895, 1260, 1261
MunI	CAATTG	2 cut(s)	984, 1014
MvaI	CCWGG	3 cut(s)	266, 414, 895
MwoI	GCNNNNNNNGC	4 cut(s)	440, 928, 1370, 1583
NciI	CCSGG	3 cut(s)	741, 1260, 1261
NdeI	CATATG	2 cut(s)	487, 1436
NdeII	GATC	5 cut(s)	207, 398, 520, 1254, 1506
NlaIII	CATG	8 cut(s)	49, 214, 292, 305, 368, 427, 434, 940
NlaIV	GGNNCC	3 cut(s)	1052, 1111, 1160
NmuCI	GTSAC	1 cut(s)	380
NspI	RCATGY	2 cut(s)	434, 940
PciI	ACATGT	1 cut(s)	936
PciSI	GCTCTTC	1 cut(s)	921
PfeI	GAWTC	6 cut(s)	147, 161, 554, 812, 1031, 1120
PfoI	TCCNGGA	1 cut(s)	739
PkrI	GCNGC	4 cut(s)	841, 1372, 1526, 1576
Ple19I	CGATCG	1 cut(s)	523
PleI	GAGTC	2 cut(s)	738, 1220
PpsI	GAGTC	2 cut(s)	738, 1220
PscI	ACATGT	1 cut(s)	936
PsiI	TTATAA	1 cut(s)	1443
Psp124BI	GAGCTC	2 cut(s)	359, 1467
Psp1406I	AACGTT	1 cut(s)	1146
Psp6I	CCWGG	3 cut(s)	264, 412, 893
PspFI	CCCAGC	1 cut(s)	1001
PspGI	CCWGG	3 cut(s)	264, 412, 893
PspN4I	GGNNCC	3 cut(s)	1052, 1111, 1160
PspPI	GGNCC	2 cut(s)	897, 1110
PstI	CTGCAG	2 cut(s)	552, 841
PstNI	CAGNNNCTG	1 cut(s)	1370
PsuI	RGATCY	1 cut(s)	1254
PvuI	CGATCG	1 cut(s)	523
PvuII	CAGCTG	1 cut(s)	1577
RsaI	GTAC	3 cut(s)	411, 429, 1338
RsaNI	GTAC	3 cut(s)	410, 428, 1337
RseI	CAYNNNNRTG	1 cut(s)	34
SacI	GAGCTC	2 cut(s)	359, 1467
SapI	GCTCTTC	1 cut(s)	921
SaqAI	TTAA	8 cut(s)	8, 20, 135, 507, 630, 783, 1164, 1487
SatI	GCNGC	4 cut(s)	840, 1371, 1525, 1575
Sau3AI	GATC	5 cut(s)	207, 398, 520, 1254, 1506
Sau96I	GGNCC	2 cut(s)	897, 1110
SchI	GAGTC	2 cut(s)	738, 1220
ScrFI	CCNGG	6 cut(s)	266, 414, 741, 895, 1260, 1261
SduI	GDGCHC	5 cut(s)	53, 71, 359, 1085, 1467
SexAI	ACCWGGT	1 cut(s)	412
SfaNI	GCATC	8 cut(s)	331, 444, 526, 571, 609, 1357, 1413, 1524
SfcI	CTRYAG	2 cut(s)	548, 837
SinI	GGWCC	2 cut(s)	897, 1110
SmaI	CCCGGG	1 cut(s)	1261
SmiMI	CAYNNNNRTG	1 cut(s)	34
SmlI	CTYRAG	2 cut(s)	1151, 1486
SmoI	CTYRAG	2 cut(s)	1151, 1486
Sse9I	AATT	7 cut(s)	167, 186, 273, 723, 984, 1014, 1089
SsiI	CCGC	5 cut(s)	191, 931, 1293, 1524, 1537
SspMI	CTAG	4 cut(s)	594, 602, 623, 1103
SstI	GAGCTC	2 cut(s)	359, 1467
StyD4I	CCNGG	6 cut(s)	264, 412, 739, 893, 1258, 1259
TaaI	ACNGT	3 cut(s)	561, 1405, 1478
TaiI	ACGT	2 cut(s)	1149, 1323
TaqI	TCGA	2 cut(s)	519, 523
TasI	AATT	7 cut(s)	167, 186, 273, 723, 984, 1014, 1089
TauI	GCSGC	1 cut(s)	1527
TfiI	GAWTC	6 cut(s)	147, 161, 554, 812, 1031, 1120
Tru1I	TTAA	8 cut(s)	8, 20, 135, 507, 630, 783, 1164, 1487
Tru9I	TTAA	8 cut(s)	8, 20, 135, 507, 630, 783, 1164, 1487
TscAI	CASTG	2 cut(s)	564, 956
TseFI	GTSAC	1 cut(s)	380
TseI	GCWGC	3 cut(s)	839, 1370, 1574
Tsp45I	GTSAC	1 cut(s)	380
TspGWI	ACGGA	2 cut(s)	379, 889
TspMI	CCCGGG	1 cut(s)	1259
TspRI	CASTG	2 cut(s)	564, 956
Vha464I	CTTAAG	1 cut(s)	1486
VpaK11BI	GGWCC	2 cut(s)	897, 1110
XagI	CCTNNNNNAGG	1 cut(s)	417
XapI	RAATTY	3 cut(s)	167, 186, 273
XbaI	TCTAGA	2 cut(s)	593, 601
XceI	RCATGY	2 cut(s)	434, 940
XcmI	CCANNNNNNNNNTGG	2 cut(s)	33, 471
XmaI	CCCGGG	1 cut(s)	1259
XmiI	GTMKAC	2 cut(s)	1007, 1473
XspI	CTAG	4 cut(s)	594, 602, 623, 1103

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.