Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0001829

Physical Location & Seq

Genomic Coordinates Reverse (-)

30329053 .. 30330957

Gene Structure 1905 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1222500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 849 bp

ATGGCTTCCTCCGCCATATCCCTCCTCTCCCCCCACAAAGCCTCCACCGCCACCCACCTTTTCCTGCGCACCCCCAAATCCCCTAACATGCGCTCACCTTCTTCCTACAGGCCCATGTCCGTCAAAGCCCGCACAGTCCCTGCCCTCACTCAAGACGACCTCAAGAAGCTAGCCGCCGACAAGGCCGTTGAGTACGTCAAATCCGGTATGGTCCTTGGCCTCGGAACTGGCTCCACTGCCGCCTTCGTGGTCTCCAAGCTCGGGGAGCTTCTCAAATCCGGCGAATTGAAGGACATCGTCGGAGTCCCGACTTCCAAGCGCACGGAGGACCAAGCTCGCCAGTTGGGTATTCCCCTTTCGGTGCTCGACGACCACCCGAAGATCGATTTGGCGATCGATGGCGCCGATGAGGTCGACCCAAATCTTGATTTGGTCAAAGGGCGCGGCGGAGCTTTGCTGAGGGAGAAGATGGTGGAGGCGGCGTCGGAGAAGTTTGTGGTGGTGGCGGATGAGACGAAGCTAGTGACTGGGCTCGGCGGAAGCGGGCTGGCAATGCCCGTGGAGGTGGTGCAGTTCTGCTGGAAGTACAACTTGGTGAGGCTTCAGGAGCTGTTTAAGGAAGAAGGGGTGGAGGCGAAATTGAGGATCGACGGCGACGGGAAGCCGTATGTGACTGATAACTTCAATTACATTGTGGATTTGTACTTCCAGACTCCGATTAAAGACGGGCCGGCGGCAGGGAAGGAGATTTTGAAATTCGAAGGAGTTGTGGAACATGGGTTGTTCTTAGACATGGCGACGGCGGTGATCATTGCGGGTACGGATGGAGTGGATGTGAAGACCAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009058 GO:0009507 GO:0009526 GO:0009532 GO:0009534 GO:0009535 GO:0009536 GO:0009570 GO:0009579 GO:0009605 GO:0009607 GO:0009617 GO:0009719 GO:0009725 GO:0009735 GO:0009941 GO:0009987 GO:0010033 GO:0015977 GO:0015979 GO:0016020 GO:0016051 GO:0019253 GO:0019685 GO:0031967 GO:0031975 GO:0031976 GO:0031984 GO:0034357 GO:0042221 GO:0042651 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0050896 GO:0051704 GO:0051707 GO:0055035 GO:0071704 GO:0098542 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00030 ko00710 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 map00030 map00710 map01100 map01110 map01120 map01130 map01200 map01230

Pfam Domains

Protein Families

Rib_5-P_isom_A

Protein Analysis

283

Amino Acids

30.28

Weight (kDa)

5.9

Isoelectric Point (pI)

25.61

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Rib_5-P_isom_A	PF06026	100 - 276	1.3e-60	Ribose 5-phosphate isomerase A (phosphoriboisomerase A)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0001829)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G04790
fragaria_vesca	FvH4_6g03160
malus_domestica	MD04G1222500.v1.1 MD12G1238600.v1.1
prunus_persica	Prupe.6G341500_v2.0.a1
pyrus_communis	pycom04g19680 pycom12g21930
rosa_chinensis	RchiOBHm_Chr3g0451421
rosa_laevigata	RLG00000007161 RLG00000007952 RLG00000016309 RLG00000017186 RLG00000025661 RLG00000034591 RLG00000035184 RLG00000035331
rosa_multiflora	Rmu_sc0000212.1_g000012 Rmu_sc0000578.1_g000020 Rmu_sc0002575.1_g000002 Rmu_sc0003216.1_g000009 Rmu_sc0004058.1_g000023 Rmu_sc0021369.1_g000003 Rmu_ssc0000421.1_g000073
rosa_roxburghii	Rroxscaffold_164G00436510 Rroxscaffold_1G00008300 Rroxscaffold_1G00041190 Rroxscaffold_2G00115660 Rroxscaffold_2G00144830 Rroxscaffold_4G00295890 Rroxscaffold_6G00428830
rosa_rugosa	Rorug02G0633700.1 Rorug05G0038600 Rorug06G0042600
rosa_samantha	Rh3AG036700 Rh3BG037300 Rh3CG035900 Rh3DG036800 Rh3DG372600 Rh4CG172100 Rh4CG229700 Rh5CG197600 Rh5DG501000 Rh5DG501100 Rh5DG501200 Rh6AG133400
rosa_wichuraiana	Rw3G002710 Rw4G018610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	68
AccB1I	GGYRCC	1 cut(s)	401
AccI	GTMKAC	1 cut(s)	414
AccII	CGCG	1 cut(s)	444
AclWI	GGATC	1 cut(s)	653
AcsI	RAATTY	1 cut(s)	755
AcuI	CTGAAG	1 cut(s)	587
AcyI	GRCGYC	2 cut(s)	402, 482
AfaI	GTAC	4 cut(s)	194, 587, 704, 820
AfiI	CCNNNNNNNGG	2 cut(s)	261, 737
AgsI	TTSAA	3 cut(s)	289, 685, 754
AjuI	GAANNNNNNNTTGG	4 cut(s)	371, 403, 575, 607
AluBI	AGCT	7 cut(s)	169, 259, 268, 335, 452, 520, 610
AluI	AGCT	7 cut(s)	169, 259, 268, 335, 452, 520, 610
Alw21I	GWGCWC	1 cut(s)	366
Alw26I	GTCTC	2 cut(s)	256, 506
AlwI	GGATC	1 cut(s)	653
AlwNI	CAGNNNCTG	2 cut(s)	140, 610
Ama87I	CYCGRG	1 cut(s)	260
AoxI	GGCC	4 cut(s)	110, 183, 217, 728
ApoI	RAATTY	1 cut(s)	755
AspLEI	GCGC	5 cut(s)	69, 93, 321, 404, 444
AspS9I	GGNCC	4 cut(s)	111, 211, 328, 728
AsuHPI	GGTGA	3 cut(s)	87, 607, 817
AsuII	TTCGAA	1 cut(s)	759
AsuNHI	GCTAGC	1 cut(s)	169
AvaI	CYCGRG	1 cut(s)	260
AvaII	GGWCC	2 cut(s)	211, 328
BanI	GGYRCC	1 cut(s)	401
BanII	GRGCYC	1 cut(s)	534
BbsI	GAAGAC	1 cut(s)	845
Bbv12I	GWGCWC	1 cut(s)	366
BbvCI	CCTCAGC	1 cut(s)	458
BccI	CCATC	3 cut(s)	392, 463, 818
BceAI	ACGGC	4 cut(s)	170, 649, 667, 816
BclI	TGATCA	1 cut(s)	807
BcoDI	GTCTC	2 cut(s)	256, 506
BfaI	CTAG	2 cut(s)	170, 521
BfmI	CTRYAG	1 cut(s)	106
BfoI	RGCGCY	1 cut(s)	405
BglI	GCCNNNNNGGC	1 cut(s)	182
BisI	GCNGC	5 cut(s)	174, 240, 445, 480, 735
BlsI	GCNGC	5 cut(s)	175, 241, 446, 481, 736
Bme18I	GGWCC	2 cut(s)	211, 328
BmeT110I	CYCGRG	1 cut(s)	260
BmgT120I	GGNCC	4 cut(s)	111, 211, 328, 728
BmiI	GGNNCC	2 cut(s)	232, 403
BmrI	ACTGGG	1 cut(s)	537
BmtI	GCTAGC	1 cut(s)	173
BmuI	ACTGGG	1 cut(s)	537
BpiI	GAAGAC	1 cut(s)	845
Bpu10I	CCTNAGC	1 cut(s)	458
Bpu14I	TTCGAA	1 cut(s)	759
BpuEI	CTTGAG	2 cut(s)	135, 146
Bsa29I	ATCGAT	2 cut(s)	384, 396
BsaHI	GRCGYC	2 cut(s)	402, 482
BsaI	GGTCTC	1 cut(s)	256
BsaJI	CCNNGG	3 cut(s)	214, 220, 558
BsaWI	WCCGGW	1 cut(s)	203
BsaXI	ACNNNNNCTCC	6 cut(s)	26, 56, 467, 497, 819, 849
Bsc4I	CCNNNNNNNGG	2 cut(s)	261, 737
Bse118I	RCCGGY	1 cut(s)	730
Bse1I	ACTGG	3 cut(s)	232, 340, 532
Bse3DI	GCAATG	2 cut(s)	558, 810
BseCI	ATCGAT	2 cut(s)	384, 396
BseDI	CCNNGG	3 cut(s)	214, 220, 558
BseGI	GGATG	3 cut(s)	514, 829, 838
BseLI	CCNNNNNNNGG	2 cut(s)	261, 737
BseMI	GCAATG	2 cut(s)	558, 810
BseMII	CTCAG	1 cut(s)	449
BseNI	ACTGG	3 cut(s)	232, 340, 532
BseRI	GAGGAG	1 cut(s)	14
BsgI	GTGCAG	1 cut(s)	590
Bsh1236I	CGCG	1 cut(s)	444
Bsh1285I	CGRYCG	1 cut(s)	396
BshFI	GGCC	4 cut(s)	112, 185, 219, 730
BshNI	GGYRCC	1 cut(s)	401
BshVI	ATCGAT	2 cut(s)	384, 396
BsiEI	CGRYCG	1 cut(s)	396
BsiHKAI	GWGCWC	1 cut(s)	366
BsiHKCI	CYCGRG	1 cut(s)	260
BsiSI	CCGG	3 cut(s)	204, 279, 731
BslFI	GGGAC	2 cut(s)	122, 290
BslI	CCNNNNNNNGG	2 cut(s)	261, 737
BsmAI	GTCTC	2 cut(s)	256, 506
BsmBI	CGTCTC	1 cut(s)	506
BsmFI	GGGAC	2 cut(s)	122, 290
BsnI	GGCC	4 cut(s)	112, 185, 219, 730
Bso31I	GGTCTC	1 cut(s)	256
BsoBI	CYCGRG	1 cut(s)	260
Bsp119I	TTCGAA	1 cut(s)	759
Bsp1286I	GDGCHC	2 cut(s)	366, 534
Bsp143I	GATC	4 cut(s)	381, 393, 645, 807
BspANI	GGCC	4 cut(s)	112, 185, 219, 730
BspCNI	CTCAG	1 cut(s)	450
BspDI	ATCGAT	2 cut(s)	384, 396
BspFNI	CGCG	1 cut(s)	444
BspLI	GGNNCC	2 cut(s)	232, 403
BspOI	GCTAGC	1 cut(s)	173
BspPI	GGATC	1 cut(s)	653
BspT104I	TTCGAA	1 cut(s)	759
BspT107I	GGYRCC	1 cut(s)	401
BspTNI	GGTCTC	1 cut(s)	256
BsrDI	GCAATG	2 cut(s)	558, 810
BsrFI	RCCGGY	1 cut(s)	730
BsrI	ACTGG	3 cut(s)	232, 340, 532
BssAI	RCCGGY	1 cut(s)	730
BssECI	CCNNGG	3 cut(s)	214, 220, 558
BssMI	GATC	4 cut(s)	381, 393, 645, 807
BssNI	GRCGYC	2 cut(s)	402, 482
BssT1I	CCWWGG	1 cut(s)	214
Bst4CI	ACNGT	1 cut(s)	136
BstACI	GRCGYC	2 cut(s)	402, 482
BstBI	TTCGAA	1 cut(s)	759
BstC8I	GCNNGC	6 cut(s)	130, 171, 337, 545, 549, 732
BstDEI	CTNAG	2 cut(s)	458, 787
BstDSI	CCRYGG	1 cut(s)	558
BstF5I	GGATG	3 cut(s)	514, 829, 838
BstFNI	CGCG	1 cut(s)	444
BstH2I	RGCGCY	1 cut(s)	405
BstHHI	GCGC	5 cut(s)	69, 93, 321, 404, 444
BstKTI	GATC	4 cut(s)	384, 396, 648, 810
BstMAI	GTCTC	2 cut(s)	256, 506
BstMBI	GATC	4 cut(s)	381, 393, 645, 807
BstMCI	CGRYCG	1 cut(s)	396
BstMWI	GCNNNNNNNGC	6 cut(s)	11, 47, 182, 265, 553, 607
BstNSI	RCATGY	1 cut(s)	91
BstSFI	CTRYAG	1 cut(s)	106
BstUI	CGCG	1 cut(s)	444
BstV2I	GAAGAC	1 cut(s)	845
Bsu15I	ATCGAT	2 cut(s)	384, 396
BsuRI	GGCC	4 cut(s)	112, 185, 219, 730
BsuTUI	ATCGAT	2 cut(s)	384, 396
BtgI	CCRYGG	1 cut(s)	558
BtsCI	GGATG	3 cut(s)	514, 829, 838
BtsI	GCAGTG	1 cut(s)	234
BtsIMutI	CAGTG	1 cut(s)	234
Cac8I	GCNNGC	6 cut(s)	130, 171, 337, 545, 549, 732
CaiI	CAGNNNCTG	2 cut(s)	140, 610
CfoI	GCGC	5 cut(s)	69, 93, 321, 404, 444
Cfr10I	RCCGGY	1 cut(s)	730
Cfr13I	GGNCC	4 cut(s)	111, 211, 328, 728
ClaI	ATCGAT	2 cut(s)	384, 396
CseI	GACGC	1 cut(s)	471
Csp6I	GTAC	4 cut(s)	193, 586, 703, 819
CspCI	CAANNNNNGTGG	2 cut(s)	540, 575
CviAII	CATG	4 cut(s)	88, 115, 776, 793
CviQI	GTAC	4 cut(s)	193, 586, 703, 819
DdeI	CTNAG	2 cut(s)	458, 787
DinI	GGCGCC	1 cut(s)	403
DpnI	GATC	4 cut(s)	383, 395, 647, 809
DpnII	GATC	4 cut(s)	381, 393, 645, 807
EciI	GGCGGA	3 cut(s)	462, 521, 552
Eco130I	CCWWGG	1 cut(s)	214
Eco24I	GRGCYC	1 cut(s)	534
Eco31I	GGTCTC	1 cut(s)	256
Eco47I	GGWCC	2 cut(s)	211, 328
Eco57I	CTGAAG	1 cut(s)	587
Eco88I	CYCGRG	1 cut(s)	260
EcoT14I	CCWWGG	1 cut(s)	214
EcoT38I	GRGCYC	1 cut(s)	534
EgeI	GGCGCC	1 cut(s)	403
EheI	GGCGCC	1 cut(s)	403
ErhI	CCWWGG	1 cut(s)	214
Esp3I	CGTCTC	1 cut(s)	506
FaeI	CATG	4 cut(s)	91, 118, 779, 796
FaiI	YATR	7 cut(s)	17, 89, 116, 209, 669, 777, 794
FalI	AAGNNNNNCTT	2 cut(s)	575, 607
FaqI	GGGAC	2 cut(s)	122, 290
FatI	CATG	4 cut(s)	87, 114, 775, 792
FauI	CCCGC	3 cut(s)	137, 536, 808
FbaI	TGATCA	1 cut(s)	807
FblI	GTMKAC	1 cut(s)	414
Fnu4HI	GCNGC	5 cut(s)	174, 240, 445, 480, 735
FokI	GGATG	3 cut(s)	521, 836, 845
FriOI	GRGCYC	1 cut(s)	534
Fsp4HI	GCNGC	5 cut(s)	174, 240, 445, 480, 735
FspBI	CTAG	2 cut(s)	170, 521
FspI	TGCGCA	1 cut(s)	68
GlaI	GCGC	5 cut(s)	68, 92, 320, 403, 443
GluI	GCNGC	5 cut(s)	174, 240, 445, 480, 735
HaeII	RGCGCY	1 cut(s)	405
HaeIII	GGCC	4 cut(s)	112, 185, 219, 730
HapII	CCGG	3 cut(s)	204, 279, 731
HgaI	GACGC	1 cut(s)	471
HhaI	GCGC	5 cut(s)	69, 93, 321, 404, 444
Hin1I	GRCGYC	2 cut(s)	402, 482
Hin1II	CATG	4 cut(s)	91, 118, 779, 796
Hin6I	GCGC	5 cut(s)	67, 91, 319, 402, 442
HinP1I	GCGC	5 cut(s)	67, 91, 319, 402, 442
HincII	GTYRAC	1 cut(s)	415
HindII	GTYRAC	1 cut(s)	415
HinfI	GANTC	2 cut(s)	303, 712
HpaII	CCGG	3 cut(s)	204, 279, 731
HphI	GGTGA	3 cut(s)	87, 607, 817
Hpy166II	GTNNAC	1 cut(s)	415
Hpy188I	TCNGA	4 cut(s)	224, 302, 487, 717
Hpy188III	TCNNGA	6 cut(s)	152, 163, 307, 425, 605, 709
Hpy8I	GTNNAC	1 cut(s)	415
Hpy99I	CGWCG	6 cut(s)	302, 371, 487, 653, 659, 802
HpyAV	CCTTC	6 cut(s)	108, 253, 283, 617, 736, 755
HpyCH4III	ACNGT	1 cut(s)	136
HpyCH4IV	ACGT	1 cut(s)	195
HpyCH4V	TGCA	1 cut(s)	571
HpyF10VI	GCNNNNNNNGC	6 cut(s)	11, 47, 182, 265, 553, 607
HpyF3I	CTNAG	2 cut(s)	458, 787
HpySE526I	ACGT	1 cut(s)	195
Hsp92I	GRCGYC	2 cut(s)	402, 482
Hsp92II	CATG	4 cut(s)	91, 118, 779, 796
HspAI	GCGC	5 cut(s)	67, 91, 319, 402, 442
KasI	GGCGCC	1 cut(s)	401
KroI	GCCGGC	1 cut(s)	730
KroNI	GCCGGC	1 cut(s)	732
Ksp22I	TGATCA	1 cut(s)	807
Kzo9I	GATC	4 cut(s)	381, 393, 645, 807
LmnI	GCTCC	4 cut(s)	236, 265, 449, 607
MaeI	CTAG	2 cut(s)	170, 521
MaeII	ACGT	1 cut(s)	195
MaeIII	GTNAC	2 cut(s)	523, 670
MalI	GATC	4 cut(s)	383, 395, 647, 809
MboI	GATC	4 cut(s)	381, 393, 645, 807
MboII	GAAGA	4 cut(s)	93, 391, 478, 632
MhlI	GDGCHC	2 cut(s)	366, 534
MluCI	AATT	4 cut(s)	284, 638, 685, 755
Mly113I	GGCGCC	1 cut(s)	402
MlyI	GAGTC	2 cut(s)	312, 706
MmeI	TCCRAC	2 cut(s)	280, 465
MroNI	GCCGGC	1 cut(s)	730
MseI	TTAA	2 cut(s)	615, 720
MspI	CCGG	3 cut(s)	204, 279, 731
MvnI	CGCG	1 cut(s)	444
MwoI	GCNNNNNNNGC	6 cut(s)	11, 47, 182, 265, 553, 607
NaeI	GCCGGC	1 cut(s)	732
NarI	GGCGCC	1 cut(s)	402
NdeII	GATC	4 cut(s)	381, 393, 645, 807
NgoMIV	GCCGGC	1 cut(s)	730
NheI	GCTAGC	1 cut(s)	169
NlaIII	CATG	4 cut(s)	91, 118, 779, 796
NlaIV	GGNNCC	2 cut(s)	232, 403
NmeAIII	GCCGAG	1 cut(s)	513
NmuCI	GTSAC	2 cut(s)	523, 670
NsbI	TGCGCA	1 cut(s)	68
NspI	RCATGY	1 cut(s)	91
NspV	TTCGAA	1 cut(s)	759
PcsI	WCGNNNNNNNCGW	1 cut(s)	402
PdiI	GCCGGC	1 cut(s)	732
PflFI	GACNNNGTC	1 cut(s)	296
PkrI	GCNGC	5 cut(s)	175, 241, 446, 481, 736
Ple19I	CGATCG	1 cut(s)	396
PleI	GAGTC	2 cut(s)	311, 706
PluTI	GGCGCC	1 cut(s)	405
PpsI	GAGTC	2 cut(s)	311, 706
PspN4I	GGNNCC	2 cut(s)	232, 403
PspPI	GGNCC	4 cut(s)	111, 211, 328, 728
PstNI	CAGNNNCTG	2 cut(s)	140, 610
PsyI	GACNNNGTC	1 cut(s)	296
PvuI	CGATCG	1 cut(s)	396
RsaI	GTAC	4 cut(s)	194, 587, 704, 820
RsaNI	GTAC	4 cut(s)	193, 586, 703, 819
SalI	GTCGAC	1 cut(s)	413
SaqAI	TTAA	2 cut(s)	615, 720
SatI	GCNGC	5 cut(s)	174, 240, 445, 480, 735
Sau3AI	GATC	4 cut(s)	381, 393, 645, 807
Sau96I	GGNCC	4 cut(s)	111, 211, 328, 728
SchI	GAGTC	2 cut(s)	312, 706
SduI	GDGCHC	2 cut(s)	366, 534
SfcI	CTRYAG	1 cut(s)	106
SfoI	GGCGCC	1 cut(s)	403
SfuI	TTCGAA	1 cut(s)	759
SinI	GGWCC	2 cut(s)	211, 328
SmlI	CTYRAG	2 cut(s)	150, 161
SmoI	CTYRAG	2 cut(s)	150, 161
Sse9I	AATT	4 cut(s)	284, 638, 685, 755
SspDI	GGCGCC	1 cut(s)	401
SspMI	CTAG	2 cut(s)	170, 521
StyI	CCWWGG	1 cut(s)	214
TaaI	ACNGT	1 cut(s)	136
TaiI	ACGT	1 cut(s)	198
TaqI	TCGA	6 cut(s)	366, 384, 396, 414, 648, 759
TasI	AATT	4 cut(s)	284, 638, 685, 755
TatI	WGTACW	2 cut(s)	585, 702
TauI	GCSGC	5 cut(s)	176, 242, 447, 482, 737
Tru1I	TTAA	2 cut(s)	615, 720
Tru9I	TTAA	2 cut(s)	615, 720
TscAI	CASTG	1 cut(s)	241
TseFI	GTSAC	2 cut(s)	523, 670
Tsp45I	GTSAC	2 cut(s)	523, 670
TspGWI	ACGGA	3 cut(s)	109, 338, 836
TspRI	CASTG	1 cut(s)	241
Tth111I	GACNNNGTC	1 cut(s)	296
VpaK11BI	GGWCC	2 cut(s)	211, 328
XapI	RAATTY	1 cut(s)	755
XceI	RCATGY	1 cut(s)	91
XmiI	GTMKAC	1 cut(s)	414
XspI	CTAG	2 cut(s)	170, 521

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.