MD04G1225300.v1.1

Pathogenesis-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0000898
Physical Location & Seq
Reverse (-)
30515863 .. 30516764
902 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1225300.v1.1.491

Sequence Viewer

Length: 456 bp
ATGGGGAAGTTAACAATTAGGCCATCCGCATCATGCACGGTGCTGCTGCTGTTGGTTTGTGTCATTATGATGGGCCGTGCCAGCGCCCAAAGTGCGAGTAACGTTAGATCGACGTACCATATCTACAATCCAGAGAAGATAGGATGGAGCTTGAACGCAGCTGGTGCTTATTGCGCCACTTGGGATGCTAATAAGCCTTTGGAATGGCGTCGGAAGTTTGGATGGACTGCTTTTTGCGGCCCCGTCGGGCCTCGGGGCCAAGCATCTTGTGGCAAGTGCTTAAGGGTGACAAACGTGAGGACAGGAGGTCAGGCAAAGGTGAGAATTGTTGATCAGTGCAGTAATGGAGGCCTAGATTTGGACGAGGGTGTGTTTAAACAACTCGACACTGATGGGAAAGGCTACGCCCAAGGCCACCTTATGGTGAACTACCAGTTTGTCAATTGTGGCGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Barwin

Protein Analysis

152

Amino Acids

16.37

Weight (kDa)

9.11

Isoelectric Point (pI)

26.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Barwin PF00967 31 - 149 1.6e-57 Barwin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 421
AciI CCGC 2 cut(s) 27, 237
AclI AACGTT 1 cut(s) 102
AcyI GRCGYC 1 cut(s) 208
AfaI GTAC 1 cut(s) 116
AfiI CCNNNNNNNGG 2 cut(s) 358, 421
AflII CTTAAG 1 cut(s) 280
AgsI TTSAA 1 cut(s) 154
AhdI GACNNNNNGTC 1 cut(s) 306
AluBI AGCT 2 cut(s) 150, 161
AluI AGCT 2 cut(s) 150, 161
Ama87I CYCGRG 1 cut(s) 252
AoxI GGCC 7 cut(s) 20, 73, 238, 248, 256, 349, 412
ApeKI GCWGC 3 cut(s) 43, 46, 158
AspLEI GCGC 2 cut(s) 86, 176
AspS9I GGNCC 4 cut(s) 73, 239, 248, 256
AsuHPI GGTGA 3 cut(s) 298, 331, 436
AvaI CYCGRG 1 cut(s) 252
BbvI GCAGC 3 cut(s) 30, 33, 170
BccI CCATC 5 cut(s) 31, 64, 138, 216, 386
BceAI ACGGC 1 cut(s) 60
BclI TGATCA 1 cut(s) 331
BfaI CTAG 1 cut(s) 353
BfoI RGCGCY 1 cut(s) 87
BfrI CTTAAG 1 cut(s) 280
BisI GCNGC 4 cut(s) 44, 47, 159, 238
BlsI GCNGC 4 cut(s) 45, 48, 160, 239
BmeRI GACNNNNNGTC 1 cut(s) 306
BmeT110I CYCGRG 1 cut(s) 252
BmgT120I GGNCC 4 cut(s) 73, 239, 248, 256
BmiI GGNNCC 2 cut(s) 241, 257
BmsI GCATC 3 cut(s) 38, 175, 272
BsaHI GRCGYC 1 cut(s) 208
BsaJI CCNNGG 2 cut(s) 251, 409
Bsc4I CCNNNNNNNGG 2 cut(s) 358, 421
Bse1I ACTGG 1 cut(s) 433
BseDI CCNNGG 2 cut(s) 251, 409
BseGI GGATG 4 cut(s) 23, 149, 190, 227
BseLI CCNNNNNNNGG 2 cut(s) 358, 421
BseNI ACTGG 1 cut(s) 433
BseXI GCAGC 3 cut(s) 30, 33, 170
BsgI GTGCAG 1 cut(s) 358
BshFI GGCC 7 cut(s) 22, 75, 240, 250, 258, 351, 414
BsiHKCI CYCGRG 1 cut(s) 252
BslI CCNNNNNNNGG 2 cut(s) 358, 421
BsnI GGCC 7 cut(s) 22, 75, 240, 250, 258, 351, 414
BsoBI CYCGRG 1 cut(s) 252
Bsp143I GATC 2 cut(s) 107, 331
BspACI CCGC 2 cut(s) 27, 237
BspANI GGCC 7 cut(s) 22, 75, 240, 250, 258, 351, 414
BspLI GGNNCC 2 cut(s) 241, 257
BspTI CTTAAG 1 cut(s) 280
BsrI ACTGG 1 cut(s) 433
BssECI CCNNGG 2 cut(s) 251, 409
BssMI GATC 2 cut(s) 107, 331
BssNI GRCGYC 1 cut(s) 208
BssT1I CCWWGG 1 cut(s) 409
Bst4CI ACNGT 1 cut(s) 40
BstACI GRCGYC 1 cut(s) 208
BstAFI CTTAAG 1 cut(s) 280
BstAPI GCANNNNNTGC 1 cut(s) 164
BstC8I GCNNGC 1 cut(s) 82
BstF5I GGATG 4 cut(s) 23, 149, 190, 227
BstH2I RGCGCY 1 cut(s) 87
BstHHI GCGC 2 cut(s) 86, 176
BstKTI GATC 2 cut(s) 110, 334
BstMBI GATC 2 cut(s) 107, 331
BstMWI GCNNNNNNNGC 4 cut(s) 81, 92, 164, 173
BstV1I GCAGC 3 cut(s) 30, 33, 170
BsuRI GGCC 7 cut(s) 22, 75, 240, 250, 258, 351, 414
BtsCI GGATG 4 cut(s) 23, 149, 190, 227
BtsIMutI CAGTG 2 cut(s) 341, 387
Cac8I GCNNGC 1 cut(s) 82
CfoI GCGC 2 cut(s) 86, 176
Cfr13I GGNCC 4 cut(s) 73, 239, 248, 256
CseI GACGC 1 cut(s) 197
Csp6I GTAC 1 cut(s) 115
CviAII CATG 1 cut(s) 33
CviQI GTAC 1 cut(s) 115
DpnI GATC 2 cut(s) 109, 333
DpnII GATC 2 cut(s) 107, 331
DraI TTTAAA 1 cut(s) 376
DriI GACNNNNNGTC 1 cut(s) 306
Eam1105I GACNNNNNGTC 1 cut(s) 306
Eco130I CCWWGG 1 cut(s) 409
Eco147I AGGCCT 1 cut(s) 351
Eco88I CYCGRG 1 cut(s) 252
EcoT14I CCWWGG 1 cut(s) 409
ErhI CCWWGG 1 cut(s) 409
FaeI CATG 1 cut(s) 36
FaiI YATR 4 cut(s) 34, 68, 120, 422
FalI AAGNNNNNCTT 2 cut(s) 402, 434
FatI CATG 1 cut(s) 32
FbaI TGATCA 1 cut(s) 331
Fnu4HI GCNGC 4 cut(s) 44, 47, 159, 238
FokI GGATG 4 cut(s) 10, 156, 197, 234
Fsp4HI GCNGC 4 cut(s) 44, 47, 159, 238
FspBI CTAG 1 cut(s) 353
GlaI GCGC 2 cut(s) 85, 175
GluI GCNGC 4 cut(s) 44, 47, 159, 238
HaeII RGCGCY 1 cut(s) 87
HaeIII GGCC 7 cut(s) 22, 75, 240, 250, 258, 351, 414
HgaI GACGC 1 cut(s) 197
HhaI GCGC 2 cut(s) 86, 176
Hin1I GRCGYC 1 cut(s) 208
Hin1II CATG 1 cut(s) 36
Hin6I GCGC 2 cut(s) 84, 174
HinP1I GCGC 2 cut(s) 84, 174
HincII GTYRAC 1 cut(s) 12
HindII GTYRAC 1 cut(s) 12
HpaI GTTAAC 1 cut(s) 12
HphI GGTGA 3 cut(s) 298, 331, 436
Hpy166II GTNNAC 2 cut(s) 12, 427
Hpy188I TCNGA 1 cut(s) 213
Hpy188III TCNNGA 1 cut(s) 131
Hpy8I GTNNAC 2 cut(s) 12, 427
Hpy99I CGWCG 3 cut(s) 115, 213, 248
HpyCH4III ACNGT 1 cut(s) 40
HpyCH4IV ACGT 3 cut(s) 102, 113, 294
HpyCH4V TGCA 2 cut(s) 36, 339
HpyF10VI GCNNNNNNNGC 4 cut(s) 81, 92, 164, 173
HpySE526I ACGT 3 cut(s) 102, 113, 294
Hsp92I GRCGYC 1 cut(s) 208
Hsp92II CATG 1 cut(s) 36
HspAI GCGC 2 cut(s) 84, 174
Ksp22I TGATCA 1 cut(s) 331
KspAI GTTAAC 1 cut(s) 12
Kzo9I GATC 2 cut(s) 107, 331
LmnI GCTCC 1 cut(s) 147
LpnPI CCDG 6 cut(s) 94, 144, 147, 288, 296, 446
Lsp1109I GCAGC 3 cut(s) 30, 33, 170
LweI GCATC 3 cut(s) 38, 175, 272
MaeI CTAG 1 cut(s) 353
MaeII ACGT 3 cut(s) 102, 113, 294
MaeIII GTNAC 2 cut(s) 98, 286
MalI GATC 2 cut(s) 109, 333
MboI GATC 2 cut(s) 107, 331
MboII GAAGA 1 cut(s) 148
MfeI CAATTG 1 cut(s) 442
MluCI AATT 3 cut(s) 15, 324, 442
MmeI TCCRAC 1 cut(s) 191
MnlI CCTC 5 cut(s) 261, 291, 299, 341, 358
MseI TTAA 4 cut(s) 11, 281, 375, 454
MslI CAYNNNNRTG 1 cut(s) 68
MspA1I CMGCKG 1 cut(s) 161
MspCI CTTAAG 1 cut(s) 280
MssI GTTTAAAC 1 cut(s) 376
MunI CAATTG 1 cut(s) 442
MwoI GCNNNNNNNGC 4 cut(s) 81, 92, 164, 173
NdeII GATC 2 cut(s) 107, 331
NlaIII CATG 1 cut(s) 36
NlaIV GGNNCC 2 cut(s) 241, 257
NmuCI GTSAC 1 cut(s) 286
PceI AGGCCT 1 cut(s) 351
PflMI CCANNNNNTGG 1 cut(s) 421
PkrI GCNGC 4 cut(s) 45, 48, 160, 239
PmeI GTTTAAAC 1 cut(s) 376
Psp1406I AACGTT 1 cut(s) 102
PspN4I GGNNCC 2 cut(s) 241, 257
PspPI GGNCC 4 cut(s) 73, 239, 248, 256
PvuII CAGCTG 1 cut(s) 161
RsaI GTAC 1 cut(s) 116
RsaNI GTAC 1 cut(s) 115
RseI CAYNNNNRTG 1 cut(s) 68
SaqAI TTAA 4 cut(s) 11, 281, 375, 454
SatI GCNGC 4 cut(s) 44, 47, 159, 238
Sau3AI GATC 2 cut(s) 107, 331
Sau96I GGNCC 4 cut(s) 73, 239, 248, 256
SetI ASST 8 cut(s) 105, 116, 152, 163, 297, 310, 321, 420
SfaNI GCATC 3 cut(s) 38, 175, 272
SmiMI CAYNNNNRTG 1 cut(s) 68
SmlI CTYRAG 1 cut(s) 280
SmoI CTYRAG 1 cut(s) 280
Sse9I AATT 3 cut(s) 15, 324, 442
SseBI AGGCCT 1 cut(s) 351
SsiI CCGC 2 cut(s) 27, 237
SspMI CTAG 1 cut(s) 353
StuI AGGCCT 1 cut(s) 351
StyI CCWWGG 1 cut(s) 409
TaaI ACNGT 1 cut(s) 40
TaiI ACGT 3 cut(s) 105, 116, 297
TaqI TCGA 2 cut(s) 110, 384
TasI AATT 3 cut(s) 15, 324, 442
TauI GCSGC 1 cut(s) 240
Tru1I TTAA 4 cut(s) 11, 281, 375, 454
Tru9I TTAA 4 cut(s) 11, 281, 375, 454
TscAI CASTG 2 cut(s) 341, 394
TseFI GTSAC 1 cut(s) 286
TseI GCWGC 3 cut(s) 43, 46, 158
Tsp45I GTSAC 1 cut(s) 286
TspRI CASTG 2 cut(s) 341, 394
Van91I CCANNNNNTGG 1 cut(s) 421
Vha464I CTTAAG 1 cut(s) 280
XcmI CCANNNNNNNNNTGG 1 cut(s) 266
XspI CTAG 1 cut(s) 353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.