Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0000898

Physical Location & Seq

Genomic Coordinates Forward (+)

30525989 .. 30526870

Gene Structure 882 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1225400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 447 bp

ATGGCCGGGAAGATCACAGCAAGCTCTGTGTTGTTTGTTTCGATCATGATCTGTGGTTTGGTCGGCAGTGCATTGGGACAAAGTGCGACGAATGTGAGAGCCACATACCACCTCTACAATCCACAGCAAAACAATTACGACTTGCGGGCAGTAAGTGCCTACTGCGCAACATGGGATGCCGATAAATCCCTCGAATGGCGCAGCAAATATGGATGGACTGCCTTTTGCGGACCTGCTGGCCCTACTGGGCAAGCCGCATGCGGAAGGTGCCTCTTGGTGACAAACACTCGGACTGGAGCTCAAGCAACGGTGAGAATTGTTGATCAGTGCAGCAACGGAGGATTGGACTTGGACGTGAATGTTTTTAACCAAATTGACACCGACGGCATTGGTTACCAACAAGGCCACCTTATTGTCAACTACGACTTTGTCGACTGCGGCGACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Barwin

Protein Analysis

149

Amino Acids

15.86

Weight (kDa)

5.12

Isoelectric Point (pI)

15.02

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Barwin	PF00967	28 - 146	4.1e-65	Barwin family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000898)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G04720
fragaria_vesca	FvH4_3g05950 FvH4_3g26010 FvH4_6g02840
malus_domestica	MD04G1225200.v1.1 MD04G1225300.v1.1 MD04G1225400.v1.1 MD12G1242000.v1.1
prunus_persica	Prupe.6G141100_v2.0.a1 Prupe.6G343900_v2.0.a1 Prupe.6G344000_v2.0.a1
pyrus_communis	pycom04g19850 pycom04g19860 pycom12g22150
rosa_chinensis	RchiOBHm_Chr3g0450761 RchiOBHm_Chr3g0450781 RchiOBHm_Chr3g0450841 RchiOBHm_Chr3g0450871 RchiOBHm_Chr3g0450891
rosa_laevigata	RLG00000025696 RLG00000025698 RLG00000025702 RLG00000025705
rosa_multiflora	Rmu_sc0000078.1_g000011 Rmu_sc0000078.1_g000016 Rmu_sc0000078.1_g000022 Rmu_sc0000078.1_g000026
rosa_roxburghii	Rroxscaffold_6G00428430 Rroxscaffold_6G00428490 Rroxscaffold_6G00428510
rosa_rugosa	Rorug02G0630400 Rorug02G0630400 Rorug02G0630400 Rorug02G0630700 Rorug02G0630900
rosa_samantha	Rh3AG031400 Rh3AG031700 Rh3AG032500 Rh3AG032600 Rh3AG033100 Rh3AG033300 Rh3BG031500 Rh3BG031900 Rh3BG032300 Rh3BG032600 Rh3BG032800 Rh3BG033000 Rh3BG033600 Rh3CG030300 Rh3CG030700 Rh3CG031000 Rh3CG031400 Rh3CG031600 Rh3CG031800 Rh3CG032300 Rh3DG031300 Rh3DG031600 Rh3DG031900 Rh3DG032200 Rh3DG032400 Rh3DG032700 Rh3DG033300
rosa_wichuraiana	Rw3G002390 Rw3G002410 Rw3G002430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	166
Acc36I	ACCTGC	1 cut(s)	241
AccB1I	GGYRCC	1 cut(s)	267
AccI	GTMKAC	1 cut(s)	432
AciI	CCGC	5 cut(s)	145, 228, 255, 261, 438
AcoI	YGGCCR	1 cut(s)	3
AfiI	CCNNNNNNNGG	1 cut(s)	195
AjiI	CACGTC	1 cut(s)	355
AluBI	AGCT	2 cut(s)	24, 299
AluI	AGCT	2 cut(s)	24, 299
Alw21I	GWGCWC	1 cut(s)	301
AoxI	GGCC	3 cut(s)	3, 238, 403
ApeKI	GCWGC	2 cut(s)	201, 330
AspLEI	GCGC	2 cut(s)	167, 201
AspS9I	GGNCC	2 cut(s)	230, 239
AsuC2I	CCSGG	1 cut(s)	7
AsuHPI	GGTGA	2 cut(s)	289, 322
AvaII	GGWCC	1 cut(s)	230
BanI	GGYRCC	1 cut(s)	267
BanII	GRGCYC	1 cut(s)	301
Bbv12I	GWGCWC	1 cut(s)	301
BbvI	GCAGC	2 cut(s)	213, 342
BccI	CCATC	1 cut(s)	207
BceAI	ACGGC	1 cut(s)	400
BclI	TGATCA	1 cut(s)	322
BcnI	CCSGG	1 cut(s)	7
BfuAI	ACCTGC	1 cut(s)	241
BisI	GCNGC	4 cut(s)	202, 255, 331, 439
BlsI	GCNGC	4 cut(s)	203, 256, 332, 440
Bme1390I	CCNGG	1 cut(s)	7
Bme18I	GGWCC	1 cut(s)	230
BmgBI	CACGTC	1 cut(s)	355
BmgT120I	GGNCC	2 cut(s)	230, 239
BmiI	GGNNCC	1 cut(s)	269
BmrFI	CCNGG	1 cut(s)	7
BmrI	ACTGGG	1 cut(s)	255
BmsI	GCATC	1 cut(s)	166
BmuI	ACTGGG	1 cut(s)	255
BpmI	CTGGAG	1 cut(s)	315
BpuEI	CTTGAG	1 cut(s)	285
BpuMI	CCSGG	1 cut(s)	7
BsaBI	GATNNNNATC	1 cut(s)	47
Bsc4I	CCNNNNNNNGG	1 cut(s)	195
Bse1I	ACTGG	2 cut(s)	250, 298
Bse8I	GATNNNNATC	1 cut(s)	47
BseGI	GGATG	2 cut(s)	181, 218
BseJI	GATNNNNATC	1 cut(s)	47
BseLI	CCNNNNNNNGG	1 cut(s)	195
BseNI	ACTGG	2 cut(s)	250, 298
BseXI	GCAGC	2 cut(s)	213, 342
BsgI	GTGCAG	1 cut(s)	349
BshFI	GGCC	3 cut(s)	5, 240, 405
BshNI	GGYRCC	1 cut(s)	267
BsiHKAI	GWGCWC	1 cut(s)	301
BsiSI	CCGG	1 cut(s)	6
BslFI	GGGAC	1 cut(s)	90
BslI	CCNNNNNNNGG	1 cut(s)	195
BsmFI	GGGAC	1 cut(s)	90
BsnI	GGCC	3 cut(s)	5, 240, 405
Bsp1286I	GDGCHC	1 cut(s)	301
Bsp143I	GATC	4 cut(s)	12, 42, 48, 322
BspACI	CCGC	5 cut(s)	145, 228, 255, 261, 438
BspANI	GGCC	3 cut(s)	5, 240, 405
BspHI	TCATGA	1 cut(s)	45
BspLI	GGNNCC	1 cut(s)	269
BspMI	ACCTGC	1 cut(s)	241
BspT107I	GGYRCC	1 cut(s)	267
BsrI	ACTGG	2 cut(s)	250, 298
BssMI	GATC	4 cut(s)	12, 42, 48, 322
Bst4CI	ACNGT	1 cut(s)	310
BstAPI	GCANNNNNTGC	1 cut(s)	155
BstC8I	GCNNGC	5 cut(s)	22, 147, 238, 252, 259
BstEII	GGTNACC	1 cut(s)	392
BstF5I	GGATG	2 cut(s)	181, 218
BstHHI	GCGC	2 cut(s)	167, 201
BstKTI	GATC	4 cut(s)	15, 45, 51, 325
BstMBI	GATC	4 cut(s)	12, 42, 48, 322
BstMWI	GCNNNNNNNGC	3 cut(s)	155, 164, 267
BstNSI	RCATGY	1 cut(s)	261
BstPI	GGTNACC	1 cut(s)	392
BstSCI	CCNGG	1 cut(s)	5
BstV1I	GCAGC	2 cut(s)	213, 342
BsuRI	GGCC	3 cut(s)	5, 240, 405
BtrI	CACGTC	1 cut(s)	355
BtsCI	GGATG	2 cut(s)	181, 218
BtsI	GCAGTG	1 cut(s)	73
BtsIMutI	CAGTG	2 cut(s)	73, 332
BveI	ACCTGC	1 cut(s)	241
Cac8I	GCNNGC	5 cut(s)	22, 147, 238, 252, 259
CciI	TCATGA	1 cut(s)	45
CfoI	GCGC	2 cut(s)	167, 201
Cfr13I	GGNCC	2 cut(s)	230, 239
CviAII	CATG	3 cut(s)	46, 171, 258
CviJI	RGCY	7 cut(s)	5, 24, 101, 240, 254, 299, 405
CviKI_1	RGCY	7 cut(s)	5, 24, 101, 240, 254, 299, 405
DpnI	GATC	4 cut(s)	14, 44, 50, 324
DpnII	GATC	4 cut(s)	12, 42, 48, 322
EaeI	YGGCCR	1 cut(s)	3
Ecl136II	GAGCTC	1 cut(s)	299
Eco24I	GRGCYC	1 cut(s)	301
Eco47I	GGWCC	1 cut(s)	230
Eco53kI	GAGCTC	1 cut(s)	299
Eco91I	GGTNACC	1 cut(s)	392
EcoICRI	GAGCTC	1 cut(s)	299
EcoO65I	GGTNACC	1 cut(s)	392
EcoT38I	GRGCYC	1 cut(s)	301
FaeI	CATG	3 cut(s)	49, 174, 261
FaiI	YATR	5 cut(s)	47, 106, 172, 210, 259
FalI	AAGNNNNNCTT	2 cut(s)	393, 425
FaqI	GGGAC	1 cut(s)	90
FatI	CATG	3 cut(s)	45, 170, 257
FauI	CCCGC	1 cut(s)	138
FbaI	TGATCA	1 cut(s)	322
FblI	GTMKAC	1 cut(s)	432
Fnu4HI	GCNGC	4 cut(s)	202, 255, 331, 439
FokI	GGATG	2 cut(s)	188, 225
FriOI	GRGCYC	1 cut(s)	301
Fsp4HI	GCNGC	4 cut(s)	202, 255, 331, 439
FspI	TGCGCA	1 cut(s)	166
GlaI	GCGC	2 cut(s)	166, 200
GluI	GCNGC	4 cut(s)	202, 255, 331, 439
GsuI	CTGGAG	1 cut(s)	315
HaeIII	GGCC	3 cut(s)	5, 240, 405
HapII	CCGG	1 cut(s)	6
HhaI	GCGC	2 cut(s)	167, 201
Hin1II	CATG	3 cut(s)	49, 174, 261
Hin6I	GCGC	2 cut(s)	165, 199
HinP1I	GCGC	2 cut(s)	165, 199
HincII	GTYRAC	2 cut(s)	418, 433
HindII	GTYRAC	2 cut(s)	418, 433
HpaII	CCGG	1 cut(s)	6
HphI	GGTGA	2 cut(s)	289, 322
Hpy166II	GTNNAC	2 cut(s)	418, 433
Hpy188I	TCNGA	1 cut(s)	291
Hpy188III	TCNNGA	1 cut(s)	46
Hpy8I	GTNNAC	2 cut(s)	418, 433
Hpy99I	CGWCG	2 cut(s)	91, 386
HpyAV	CCTTC	1 cut(s)	258
HpyCH4III	ACNGT	1 cut(s)	310
HpyCH4IV	ACGT	1 cut(s)	354
HpyCH4V	TGCA	2 cut(s)	71, 330
HpyF10VI	GCNNNNNNNGC	3 cut(s)	155, 164, 267
HpySE526I	ACGT	1 cut(s)	354
Hsp92II	CATG	3 cut(s)	49, 174, 261
HspAI	GCGC	2 cut(s)	165, 199
Ksp22I	TGATCA	1 cut(s)	322
Kzo9I	GATC	4 cut(s)	12, 42, 48, 322
LmnI	GCTCC	1 cut(s)	296
LpnPI	CCDG	5 cut(s)	19, 222, 231, 246, 279
Lsp1109I	GCAGC	2 cut(s)	213, 342
LweI	GCATC	1 cut(s)	166
MaeII	ACGT	1 cut(s)	354
MaeIII	GTNAC	2 cut(s)	277, 392
MalI	GATC	4 cut(s)	14, 44, 50, 324
MboI	GATC	4 cut(s)	12, 42, 48, 322
MboII	GAAGA	1 cut(s)	22
MhlI	GDGCHC	1 cut(s)	301
MluCI	AATT	3 cut(s)	133, 315, 372
MnlI	CCTC	4 cut(s)	122, 200, 281, 332
MseI	TTAA	1 cut(s)	366
MspI	CCGG	1 cut(s)	6
MspR9I	CCNGG	1 cut(s)	7
MwoI	GCNNNNNNNGC	3 cut(s)	155, 164, 267
NciI	CCSGG	1 cut(s)	7
NdeII	GATC	4 cut(s)	12, 42, 48, 322
NlaIII	CATG	3 cut(s)	49, 174, 261
NlaIV	GGNNCC	1 cut(s)	269
NmuCI	GTSAC	1 cut(s)	277
NsbI	TGCGCA	1 cut(s)	166
NspI	RCATGY	1 cut(s)	261
PaeI	GCATGC	1 cut(s)	261
PagI	TCATGA	1 cut(s)	45
PcsI	WCGNNNNNNNCGW	2 cut(s)	429, 438
PflFI	GACNNNGTC	1 cut(s)	428
PkrI	GCNGC	4 cut(s)	203, 256, 332, 440
Psp124BI	GAGCTC	1 cut(s)	301
PspEI	GGTNACC	1 cut(s)	392
PspN4I	GGNNCC	1 cut(s)	269
PspPI	GGNCC	2 cut(s)	230, 239
PsyI	GACNNNGTC	1 cut(s)	428
SacI	GAGCTC	1 cut(s)	301
SalI	GTCGAC	1 cut(s)	431
SaqAI	TTAA	1 cut(s)	366
SatI	GCNGC	4 cut(s)	202, 255, 331, 439
Sau3AI	GATC	4 cut(s)	12, 42, 48, 322
Sau96I	GGNCC	2 cut(s)	230, 239
ScrFI	CCNGG	1 cut(s)	7
SduI	GDGCHC	1 cut(s)	301
SetI	ASST	7 cut(s)	26, 114, 235, 269, 301, 357, 411
SfaNI	GCATC	1 cut(s)	166
SinI	GGWCC	1 cut(s)	230
SmlI	CTYRAG	1 cut(s)	300
SmoI	CTYRAG	1 cut(s)	300
SphI	GCATGC	1 cut(s)	261
Sse9I	AATT	3 cut(s)	133, 315, 372
SsiI	CCGC	5 cut(s)	145, 228, 255, 261, 438
SstI	GAGCTC	1 cut(s)	301
StyD4I	CCNGG	1 cut(s)	5
TaaI	ACNGT	1 cut(s)	310
TaiI	ACGT	1 cut(s)	357
TaqI	TCGA	3 cut(s)	41, 192, 432
TasI	AATT	3 cut(s)	133, 315, 372
TauI	GCSGC	2 cut(s)	257, 441
Tru1I	TTAA	1 cut(s)	366
Tru9I	TTAA	1 cut(s)	366
TscAI	CASTG	2 cut(s)	73, 332
TseFI	GTSAC	1 cut(s)	277
TseI	GCWGC	2 cut(s)	201, 330
Tsp45I	GTSAC	1 cut(s)	277
TspGWI	ACGGA	1 cut(s)	351
TspRI	CASTG	2 cut(s)	73, 332
Tth111I	GACNNNGTC	1 cut(s)	428
VpaK11BI	GGWCC	1 cut(s)	230
XceI	RCATGY	1 cut(s)	261
XmiI	GTMKAC	1 cut(s)	432

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.