MD12G1242000.v1.1

Pathogenesis-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0000898
Physical Location & Seq
Forward (+)
31358613 .. 31359209
597 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1242000.v1.1.491

Sequence Viewer

Length: 444 bp
ATGGCAGGGAAGATTGCACCATGGTTTGTATTGTTAGTTTCAGTCATTATCTGTGTATTTGTGGGAAGTGCGTGGGGACAAAGTGCTACTGTGGGATCCACGTACCACCTCTACAATCCAGCCCAAATCAATTACGACTTGCGGGCTGTTAGTGCATTCTGCTCAACATGGGATGCCGATCAATCCTACGAATGGCGCAGCAAATATGGATGGACTGCATTTTGCGGACCTGCTGGCCCTAGCGGAGAAGACGCTTGCGGAAAGTGCCTCTTGGTGACAAACACTGTGACAGGAGCACAAACAGTGGTGAGAATTATTGATCAATGCAGCAACGGAGGGCTAGACTTGGACGTGAATATGTTTAACCAAATTGACACCGACGGCAGTGGCTACGCATCAGGCCACCTTAACGTCAACTACGATTTTGTCGACTGCAGCGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Barwin

Protein Analysis

148

Amino Acids

15.82

Weight (kDa)

4.31

Isoelectric Point (pI)

20.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Barwin PF00967 30 - 145 3.7e-57 Barwin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 238
AccI GTMKAC 1 cut(s) 429
AciI CCGC 4 cut(s) 142, 225, 243, 258
AclWI GGATC 2 cut(s) 90, 103
AfaI GTAC 1 cut(s) 104
AfiI CCNNNNNNNGG 1 cut(s) 192
AjiI CACGTC 1 cut(s) 352
Alw21I GWGCWC 1 cut(s) 298
AlwI GGATC 2 cut(s) 90, 103
AoxI GGCC 2 cut(s) 235, 400
ApeKI GCWGC 3 cut(s) 198, 327, 435
AspLEI GCGC 1 cut(s) 198
AspS9I GGNCC 2 cut(s) 227, 236
AsuHPI GGTGA 2 cut(s) 286, 319
AvaII GGWCC 1 cut(s) 227
BamHI GGATCC 1 cut(s) 95
BbsI GAAGAC 1 cut(s) 255
Bbv12I GWGCWC 1 cut(s) 298
BbvI GCAGC 2 cut(s) 210, 339
BccI CCATC 1 cut(s) 204
BceAI ACGGC 1 cut(s) 397
BclI TGATCA 1 cut(s) 319
BfaI CTAG 2 cut(s) 240, 341
BfmI CTRYAG 1 cut(s) 433
BfuAI ACCTGC 1 cut(s) 238
BisI GCNGC 3 cut(s) 199, 328, 436
BlsI GCNGC 3 cut(s) 200, 329, 437
Bme18I GGWCC 1 cut(s) 227
BmgBI CACGTC 1 cut(s) 352
BmgT120I GGNCC 2 cut(s) 227, 236
BmiI GGNNCC 1 cut(s) 97
BmsI GCATC 2 cut(s) 163, 404
BpiI GAAGAC 1 cut(s) 255
BsaAI YACGTR 1 cut(s) 102
BsaBI GATNNNNATC 1 cut(s) 177
BsaJI CCNNGG 1 cut(s) 20
Bsc4I CCNNNNNNNGG 1 cut(s) 192
Bse8I GATNNNNATC 1 cut(s) 177
BseDI CCNNGG 1 cut(s) 20
BseGI GGATG 2 cut(s) 178, 215
BseJI GATNNNNATC 1 cut(s) 177
BseLI CCNNNNNNNGG 1 cut(s) 192
BseXI GCAGC 2 cut(s) 210, 339
BshFI GGCC 2 cut(s) 237, 402
BsiHKAI GWGCWC 1 cut(s) 298
BslFI GGGAC 1 cut(s) 90
BslI CCNNNNNNNGG 1 cut(s) 192
BsmFI GGGAC 1 cut(s) 90
BsmI GAATGC 1 cut(s) 155
BsnI GGCC 2 cut(s) 237, 402
Bsp1286I GDGCHC 1 cut(s) 298
Bsp143I GATC 3 cut(s) 95, 178, 319
Bsp19I CCATGG 1 cut(s) 20
BspACI CCGC 4 cut(s) 142, 225, 243, 258
BspANI GGCC 2 cut(s) 237, 402
BspLI GGNNCC 1 cut(s) 97
BspMAI CTGCAG 1 cut(s) 437
BspMI ACCTGC 1 cut(s) 238
BspPI GGATC 2 cut(s) 90, 103
BssECI CCNNGG 1 cut(s) 20
BssMI GATC 3 cut(s) 95, 178, 319
BssT1I CCWWGG 1 cut(s) 20
Bst4CI ACNGT 3 cut(s) 91, 286, 304
BstBAI YACGTR 1 cut(s) 102
BstC8I GCNNGC 3 cut(s) 144, 235, 256
BstDSI CCRYGG 1 cut(s) 20
BstF5I GGATG 2 cut(s) 178, 215
BstHHI GCGC 1 cut(s) 198
BstKTI GATC 3 cut(s) 98, 181, 322
BstMBI GATC 3 cut(s) 95, 178, 319
BstMWI GCNNNNNNNGC 2 cut(s) 152, 264
BstSFI CTRYAG 1 cut(s) 433
BstV1I GCAGC 2 cut(s) 210, 339
BstV2I GAAGAC 1 cut(s) 255
BstX2I RGATCY 1 cut(s) 95
BstYI RGATCY 1 cut(s) 95
BsuRI GGCC 2 cut(s) 237, 402
BtgI CCRYGG 1 cut(s) 20
BtrI CACGTC 1 cut(s) 352
BtsCI GGATG 2 cut(s) 178, 215
BtsI GCAGTG 1 cut(s) 391
BtsIMutI CAGTG 3 cut(s) 282, 309, 391
BveI ACCTGC 1 cut(s) 238
Cac8I GCNNGC 3 cut(s) 144, 235, 256
CfoI GCGC 1 cut(s) 198
Cfr13I GGNCC 2 cut(s) 227, 236
CseI GACGC 1 cut(s) 260
Csp6I GTAC 1 cut(s) 103
CviAII CATG 2 cut(s) 21, 168
CviJI RGCY 6 cut(s) 122, 146, 237, 340, 390, 402
CviKI_1 RGCY 6 cut(s) 122, 146, 237, 340, 390, 402
CviQI GTAC 1 cut(s) 103
DpnI GATC 3 cut(s) 97, 180, 321
DpnII GATC 3 cut(s) 95, 178, 319
Eco130I CCWWGG 1 cut(s) 20
Eco47I GGWCC 1 cut(s) 227
EcoT14I CCWWGG 1 cut(s) 20
ErhI CCWWGG 1 cut(s) 20
FaeI CATG 2 cut(s) 24, 171
FaiI YATR 4 cut(s) 22, 169, 207, 359
FalI AAGNNNNNCTT 2 cut(s) 254, 286
FaqI GGGAC 1 cut(s) 90
FatI CATG 2 cut(s) 20, 167
FauI CCCGC 1 cut(s) 135
FbaI TGATCA 1 cut(s) 319
FblI GTMKAC 1 cut(s) 429
Fnu4HI GCNGC 3 cut(s) 199, 328, 436
FokI GGATG 2 cut(s) 185, 222
Fsp4HI GCNGC 3 cut(s) 199, 328, 436
FspBI CTAG 2 cut(s) 240, 341
GlaI GCGC 1 cut(s) 197
GluI GCNGC 3 cut(s) 199, 328, 436
HaeIII GGCC 2 cut(s) 237, 402
HgaI GACGC 1 cut(s) 260
HhaI GCGC 1 cut(s) 198
Hin1II CATG 2 cut(s) 24, 171
Hin6I GCGC 1 cut(s) 196
HinP1I GCGC 1 cut(s) 196
HincII GTYRAC 2 cut(s) 415, 430
HindII GTYRAC 2 cut(s) 415, 430
HphI GGTGA 2 cut(s) 286, 319
Hpy166II GTNNAC 2 cut(s) 415, 430
Hpy8I GTNNAC 2 cut(s) 415, 430
Hpy99I CGWCG 1 cut(s) 383
HpyCH4III ACNGT 3 cut(s) 91, 286, 304
HpyCH4IV ACGT 3 cut(s) 101, 351, 411
HpyCH4V TGCA 5 cut(s) 17, 155, 218, 327, 435
HpyF10VI GCNNNNNNNGC 2 cut(s) 152, 264
HpySE526I ACGT 3 cut(s) 101, 351, 411
Hsp92II CATG 2 cut(s) 24, 171
HspAI GCGC 1 cut(s) 196
Ksp22I TGATCA 1 cut(s) 319
Kzo9I GATC 3 cut(s) 95, 178, 319
LmnI GCTCC 1 cut(s) 293
LpnPI CCDG 5 cut(s) 132, 219, 243, 276, 384
Lsp1109I GCAGC 2 cut(s) 210, 339
LweI GCATC 2 cut(s) 163, 404
MaeI CTAG 2 cut(s) 240, 341
MaeII ACGT 3 cut(s) 101, 351, 411
MaeIII GTNAC 2 cut(s) 274, 286
MalI GATC 3 cut(s) 97, 180, 321
MboI GATC 3 cut(s) 95, 178, 319
MboII GAAGA 2 cut(s) 22, 260
MflI RGATCY 1 cut(s) 95
MhlI GDGCHC 1 cut(s) 298
MluCI AATT 3 cut(s) 130, 312, 369
MnlI CCTC 3 cut(s) 119, 278, 329
MseI TTAA 2 cut(s) 363, 408
Mva1269I GAATGC 1 cut(s) 155
MwoI GCNNNNNNNGC 2 cut(s) 152, 264
NcoI CCATGG 1 cut(s) 20
NdeII GATC 3 cut(s) 95, 178, 319
NlaIII CATG 2 cut(s) 24, 171
NlaIV GGNNCC 1 cut(s) 97
NmuCI GTSAC 2 cut(s) 274, 286
PcsI WCGNNNNNNNCGW 3 cut(s) 417, 426, 435
PctI GAATGC 1 cut(s) 155
PkrI GCNGC 3 cut(s) 200, 329, 437
Ppu21I YACGTR 1 cut(s) 102
PspN4I GGNNCC 1 cut(s) 97
PspPI GGNCC 2 cut(s) 227, 236
PstI CTGCAG 1 cut(s) 437
PsuI RGATCY 1 cut(s) 95
RsaI GTAC 1 cut(s) 104
RsaNI GTAC 1 cut(s) 103
SalI GTCGAC 1 cut(s) 428
SaqAI TTAA 2 cut(s) 363, 408
SatI GCNGC 3 cut(s) 199, 328, 436
Sau3AI GATC 3 cut(s) 95, 178, 319
Sau96I GGNCC 2 cut(s) 227, 236
SduI GDGCHC 1 cut(s) 298
SetI ASST 6 cut(s) 104, 111, 232, 354, 408, 414
SfaNI GCATC 2 cut(s) 163, 404
SfcI CTRYAG 1 cut(s) 433
SinI GGWCC 1 cut(s) 227
Sse9I AATT 3 cut(s) 130, 312, 369
SsiI CCGC 4 cut(s) 142, 225, 243, 258
SspMI CTAG 2 cut(s) 240, 341
StyI CCWWGG 1 cut(s) 20
TaaI ACNGT 3 cut(s) 91, 286, 304
TaiI ACGT 3 cut(s) 104, 354, 414
TaqI TCGA 1 cut(s) 429
TasI AATT 3 cut(s) 130, 312, 369
Tru1I TTAA 2 cut(s) 363, 408
Tru9I TTAA 2 cut(s) 363, 408
TscAI CASTG 3 cut(s) 289, 309, 391
TseFI GTSAC 2 cut(s) 274, 286
TseI GCWGC 3 cut(s) 198, 327, 435
Tsp45I GTSAC 2 cut(s) 274, 286
TspGWI ACGGA 1 cut(s) 348
TspRI CASTG 3 cut(s) 289, 309, 391
VpaK11BI GGWCC 1 cut(s) 227
XmiI GTMKAC 1 cut(s) 429
XspI CTAG 2 cut(s) 240, 341
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.