MD05G1153200.v1.1
NAC Family

Probably inactive leucine-rich repeat receptor-like protein kinase At5g48380

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0003453
Physical Location & Seq
Reverse (-)
28407135 .. 28409798
2664 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1153200.v1.1.491

Sequence Viewer

Length: 819 bp
ATGTTAGAGAGGTCACATAATCGGTCATGGTATTGGGTTCATTTTCTGTTTCTAGCTTGTAGCTTCTGCTTTGCTGTCGAGAGCGATATCAATTGCTTAAAAGGTATTAAAGACTCGCTTAACGACCCTTTAGGCTACTTAAACTCTTCATGGGATTTCAAAAACAAGACCGAAGGTTTCATCTGTAACTTTCTGGGAATCGAGTGCTGGCACCCTCACGAGAGCAAAGTTTTAAATATCAAGCTTTCGGATTTGGGACTCAAGGGCCAGTTTCCTCGGGGTGTAGCAAACTGCACAAGCTTAACAGGCCTAGATCTTTCAAGCAACATGCTTAACGGACCTCTTCCTACTGATATTGGTAGATTCCTTCCGTTCCTTACTTATCTTGATCTCTCATCCAACAGCTTCTCCGGGTCAATCCCAGCAAATCTTTCCAATTGTACCTATATGAATGTGCTCATGCTTGATAACAATAAGTTTACGGGTAAAATTCCTCCACAGCTTAGCCAGCTCTCTAGGCTTACAAGATTTACCGTGGCCAACAATCTTCTGTCTGGTCAAGTCCCAGACTTTGGTAATGCTACTGTTTTTATCACAACGAACAATACTGGACTCTGCGGGGGCATGCTGACGCCTTGCCGGTCTTCTCAAGTGAAGACGTCCATTGGTCTAGTTAAGGTTTTGAAGTCGCACAGTGTTGTGATAACAGCAGCAGCTGGTTTTGGATTTGCTTTTCCTTTGTCTTTCTGTTTTTTCCTTCCAAGAGCTCCCAGTGTGCGCAGTTTGTGGGTCTTTTATCAAGGATTTAAGCTGAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8

Protein Analysis

273

Amino Acids

30.11

Weight (kDa)

9.04

Isoelectric Point (pI)

37.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 27 - 70 5.7e-06 Leucine rich repeat N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 662
Acc16I TGCGCA 1 cut(s) 779
AccB1I GGYRCC 1 cut(s) 210
AccB7I CCANNNNNTGG 1 cut(s) 572
AciI CCGC 1 cut(s) 618
AcoI YGGCCR 1 cut(s) 537
AcsI RAATTY 1 cut(s) 489
AcyI GRCGYC 2 cut(s) 632, 659
AfaI GTAC 1 cut(s) 442
AfiI CCNNNNNNNGG 1 cut(s) 572
AgsI TTSAA 3 cut(s) 160, 321, 685
Alw21I GWGCWC 2 cut(s) 459, 769
AlwNI CAGNNNCTG 1 cut(s) 716
Ama87I CYCGRG 1 cut(s) 276
AoxI GGCC 3 cut(s) 265, 307, 537
ApeKI GCWGC 2 cut(s) 710, 713
ApoI RAATTY 1 cut(s) 489
AspLEI GCGC 1 cut(s) 780
AspS9I GGNCC 2 cut(s) 265, 338
AsuC2I CCSGG 1 cut(s) 412
AvaI CYCGRG 1 cut(s) 276
AvaII GGWCC 1 cut(s) 338
BalI TGGCCA 1 cut(s) 539
BanI GGYRCC 1 cut(s) 210
BanII GRGCYC 1 cut(s) 769
BauI CACGAG 1 cut(s) 218
BbsI GAAGAC 2 cut(s) 636, 662
Bbv12I GWGCWC 2 cut(s) 459, 769
BbvCI CCTCAGC 1 cut(s) 812
BbvI GCAGC 2 cut(s) 722, 725
BcnI CCSGG 1 cut(s) 412
BfaI CTAG 4 cut(s) 53, 311, 516, 671
BglII AGATCT 1 cut(s) 313
BisI GCNGC 2 cut(s) 711, 714
BlpI GCTNAGC 1 cut(s) 503
BlsI GCNGC 2 cut(s) 712, 715
Bme1390I CCNGG 1 cut(s) 412
Bme18I GGWCC 1 cut(s) 338
BmeT110I CYCGRG 1 cut(s) 276
BmgT120I GGNCC 2 cut(s) 265, 338
BmiI GGNNCC 1 cut(s) 212
BmrFI CCNGG 1 cut(s) 412
BmrI ACTGGG 1 cut(s) 765
BmuI ACTGGG 1 cut(s) 765
BpiI GAAGAC 2 cut(s) 636, 662
Bpu10I CCTNAGC 1 cut(s) 812
Bpu1102I GCTNAGC 1 cut(s) 503
BpuEI CTTGAG 2 cut(s) 245, 633
BpuMI CCSGG 1 cut(s) 412
BsaHI GRCGYC 2 cut(s) 632, 659
BsaJI CCNNGG 2 cut(s) 275, 534
BsaXI ACNNNNNCTCC 2 cut(s) 392, 422
Bsc4I CCNNNNNNNGG 1 cut(s) 572
Bse118I RCCGGY 1 cut(s) 639
Bse1I ACTGG 3 cut(s) 268, 613, 771
BseDI CCNNGG 2 cut(s) 275, 534
BseGI GGATG 1 cut(s) 395
BseLI CCNNNNNNNGG 1 cut(s) 572
BseMII CTCAG 1 cut(s) 803
BseNI ACTGG 3 cut(s) 268, 613, 771
BseXI GCAGC 2 cut(s) 722, 725
BseYI CCCAGC 1 cut(s) 421
BsgI GTGCAG 1 cut(s) 277
BshFI GGCC 3 cut(s) 267, 309, 539
BshNI GGYRCC 1 cut(s) 210
BsiHKAI GWGCWC 2 cut(s) 459, 769
BsiHKCI CYCGRG 1 cut(s) 276
BsiSI CCGG 2 cut(s) 411, 640
BslFI GGGAC 2 cut(s) 270, 548
BslI CCNNNNNNNGG 1 cut(s) 572
BsmFI GGGAC 2 cut(s) 270, 548
BsnI GGCC 3 cut(s) 267, 309, 539
BsoBI CYCGRG 1 cut(s) 276
Bsp1286I GDGCHC 2 cut(s) 459, 769
Bsp143I GATC 2 cut(s) 313, 388
Bsp1720I GCTNAGC 1 cut(s) 503
BspACI CCGC 1 cut(s) 618
BspANI GGCC 3 cut(s) 267, 309, 539
BspCNI CTCAG 1 cut(s) 804
BspLI GGNNCC 1 cut(s) 212
BspT107I GGYRCC 1 cut(s) 210
BsrFI RCCGGY 1 cut(s) 639
BsrI ACTGG 3 cut(s) 268, 613, 771
BssAI RCCGGY 1 cut(s) 639
BssECI CCNNGG 2 cut(s) 275, 534
BssMI GATC 2 cut(s) 313, 388
BssNI GRCGYC 2 cut(s) 632, 659
BssSI CACGAG 1 cut(s) 218
Bst2BI CACGAG 1 cut(s) 218
Bst4CI ACNGT 3 cut(s) 535, 586, 695
Bst6I CTCTTC 2 cut(s) 151, 348
BstACI GRCGYC 2 cut(s) 632, 659
BstC8I GCNNGC 3 cut(s) 209, 509, 626
BstDEI CTNAG 2 cut(s) 503, 812
BstDSI CCRYGG 1 cut(s) 534
BstF5I GGATG 1 cut(s) 395
BstHHI GCGC 1 cut(s) 780
BstKTI GATC 2 cut(s) 316, 391
BstMBI GATC 2 cut(s) 313, 388
BstMWI GCNNNNNNNGC 3 cut(s) 306, 508, 517
BstNSI RCATGY 2 cut(s) 331, 628
BstSCI CCNGG 1 cut(s) 410
BstV1I GCAGC 2 cut(s) 722, 725
BstV2I GAAGAC 2 cut(s) 636, 662
BstX2I RGATCY 1 cut(s) 313
BstYI RGATCY 1 cut(s) 313
BsuRI GGCC 3 cut(s) 267, 309, 539
BtgI CCRYGG 1 cut(s) 534
BtsCI GGATG 1 cut(s) 395
BtsIMutI CAGTG 2 cut(s) 700, 778
Cac8I GCNNGC 3 cut(s) 209, 509, 626
CaiI CAGNNNCTG 1 cut(s) 716
CfoI GCGC 1 cut(s) 780
Cfr10I RCCGGY 1 cut(s) 639
Cfr13I GGNCC 2 cut(s) 265, 338
CseI GACGC 1 cut(s) 640
Csp6I GTAC 1 cut(s) 441
CviAII CATG 5 cut(s) 27, 150, 328, 460, 625
CviQI GTAC 1 cut(s) 441
DdeI CTNAG 2 cut(s) 503, 812
DpnI GATC 2 cut(s) 315, 390
DpnII GATC 2 cut(s) 313, 388
DraI TTTAAA 1 cut(s) 234
EaeI YGGCCR 1 cut(s) 537
Eam1104I CTCTTC 2 cut(s) 151, 348
EarI CTCTTC 2 cut(s) 151, 348
Ecl136II GAGCTC 1 cut(s) 767
Eco147I AGGCCT 1 cut(s) 309
Eco24I GRGCYC 1 cut(s) 769
Eco32I GATATC 1 cut(s) 88
Eco47I GGWCC 1 cut(s) 338
Eco53kI GAGCTC 1 cut(s) 767
Eco88I CYCGRG 1 cut(s) 276
EcoICRI GAGCTC 1 cut(s) 767
EcoRV GATATC 1 cut(s) 88
EcoT38I GRGCYC 1 cut(s) 769
FaeI CATG 5 cut(s) 30, 153, 331, 463, 628
FaiI YATR 8 cut(s) 18, 28, 151, 329, 447, 449, 461, 626
FalI AAGNNNNNCTT 2 cut(s) 102, 134
FaqI GGGAC 2 cut(s) 270, 548
FatI CATG 5 cut(s) 26, 149, 327, 459, 624
FauI CCCGC 1 cut(s) 611
Fnu4HI GCNGC 2 cut(s) 711, 714
FokI GGATG 1 cut(s) 382
FriOI GRGCYC 1 cut(s) 769
Fsp4HI GCNGC 2 cut(s) 711, 714
FspBI CTAG 4 cut(s) 53, 311, 516, 671
FspI TGCGCA 1 cut(s) 779
GlaI GCGC 1 cut(s) 779
GluI GCNGC 2 cut(s) 711, 714
GsaI CCCAGC 1 cut(s) 425
HaeIII GGCC 3 cut(s) 267, 309, 539
HapII CCGG 2 cut(s) 411, 640
HgaI GACGC 1 cut(s) 640
HhaI GCGC 1 cut(s) 780
Hin1I GRCGYC 2 cut(s) 632, 659
Hin1II CATG 5 cut(s) 30, 153, 331, 463, 628
Hin6I GCGC 1 cut(s) 778
HinP1I GCGC 1 cut(s) 778
HindIII AAGCTT 2 cut(s) 242, 298
HinfI GANTC 5 cut(s) 113, 198, 258, 363, 612
HpaII CCGG 2 cut(s) 411, 640
Hpy166II GTNNAC 1 cut(s) 480
Hpy188I TCNGA 1 cut(s) 250
Hpy188III TCNNGA 3 cut(s) 79, 218, 386
Hpy8I GTNNAC 1 cut(s) 480
HpyAV CCTTC 3 cut(s) 167, 377, 767
HpyCH4III ACNGT 3 cut(s) 535, 586, 695
HpyCH4IV ACGT 1 cut(s) 659
HpyCH4V TGCA 1 cut(s) 294
HpyF10VI GCNNNNNNNGC 3 cut(s) 306, 508, 517
HpyF3I CTNAG 2 cut(s) 503, 812
HpySE526I ACGT 1 cut(s) 659
Hsp92I GRCGYC 2 cut(s) 632, 659
Hsp92II CATG 5 cut(s) 30, 153, 331, 463, 628
HspAI GCGC 1 cut(s) 778
Kzo9I GATC 2 cut(s) 313, 388
LmnI GCTCC 1 cut(s) 772
Lsp1109I GCAGC 2 cut(s) 722, 725
MaeI CTAG 4 cut(s) 53, 311, 516, 671
MaeII ACGT 1 cut(s) 659
MaeIII GTNAC 2 cut(s) 12, 185
MalI GATC 2 cut(s) 315, 390
MboI GATC 2 cut(s) 313, 388
MboII GAAGA 5 cut(s) 138, 335, 539, 636, 667
MfeI CAATTG 2 cut(s) 91, 436
MflI RGATCY 1 cut(s) 313
MhlI GDGCHC 2 cut(s) 459, 769
MlsI TGGCCA 1 cut(s) 539
MluCI AATT 3 cut(s) 91, 436, 489
MluNI TGGCCA 1 cut(s) 539
MlyI GAGTC 3 cut(s) 107, 252, 606
MmeI TCCRAC 1 cut(s) 423
MnlI CCTC 6 cut(s) 3, 225, 285, 351, 504, 807
Mox20I TGGCCA 1 cut(s) 539
MscI TGGCCA 1 cut(s) 539
MseI TTAA 9 cut(s) 98, 108, 120, 140, 233, 302, 333, 675, 807
Msp20I TGGCCA 1 cut(s) 539
MspA1I CMGCKG 1 cut(s) 716
MspI CCGG 2 cut(s) 411, 640
MspR9I CCNGG 1 cut(s) 412
MunI CAATTG 2 cut(s) 91, 436
MwoI GCNNNNNNNGC 3 cut(s) 306, 508, 517
NciI CCSGG 1 cut(s) 412
NdeII GATC 2 cut(s) 313, 388
NlaIII CATG 5 cut(s) 30, 153, 331, 463, 628
NlaIV GGNNCC 1 cut(s) 212
NmuCI GTSAC 1 cut(s) 12
NsbI TGCGCA 1 cut(s) 779
NspI RCATGY 2 cut(s) 331, 628
PaeI GCATGC 1 cut(s) 628
PceI AGGCCT 1 cut(s) 309
PfeI GAWTC 2 cut(s) 198, 363
PflMI CCANNNNNTGG 1 cut(s) 572
PkrI GCNGC 2 cut(s) 712, 715
PleI GAGTC 3 cut(s) 107, 252, 606
PpsI GAGTC 3 cut(s) 107, 252, 606
Psp124BI GAGCTC 1 cut(s) 769
PspFI CCCAGC 1 cut(s) 421
PspN4I GGNNCC 1 cut(s) 212
PspPI GGNCC 2 cut(s) 265, 338
PstNI CAGNNNCTG 1 cut(s) 716
PsuI RGATCY 1 cut(s) 313
PvuII CAGCTG 1 cut(s) 716
RsaI GTAC 1 cut(s) 442
RsaNI GTAC 1 cut(s) 441
SacI GAGCTC 1 cut(s) 769
SaqAI TTAA 9 cut(s) 98, 108, 120, 140, 233, 302, 333, 675, 807
SatI GCNGC 2 cut(s) 711, 714
Sau3AI GATC 2 cut(s) 313, 388
Sau96I GGNCC 2 cut(s) 265, 338
SchI GAGTC 3 cut(s) 107, 252, 606
ScrFI CCNGG 1 cut(s) 412
SduI GDGCHC 2 cut(s) 459, 769
SinI GGWCC 1 cut(s) 338
SmlI CTYRAG 2 cut(s) 260, 648
SmoI CTYRAG 2 cut(s) 260, 648
SphI GCATGC 1 cut(s) 628
Sse9I AATT 3 cut(s) 91, 436, 489
SseBI AGGCCT 1 cut(s) 309
SsiI CCGC 1 cut(s) 618
SspMI CTAG 4 cut(s) 53, 311, 516, 671
SstI GAGCTC 1 cut(s) 769
StuI AGGCCT 1 cut(s) 309
StyD4I CCNGG 1 cut(s) 410
TaaI ACNGT 3 cut(s) 535, 586, 695
TaiI ACGT 1 cut(s) 662
TaqI TCGA 2 cut(s) 78, 201
TaqII GACCGA 2 cut(s) 12, 185
TasI AATT 3 cut(s) 91, 436, 489
TfiI GAWTC 2 cut(s) 198, 363
Tru1I TTAA 9 cut(s) 98, 108, 120, 140, 233, 302, 333, 675, 807
Tru9I TTAA 9 cut(s) 98, 108, 120, 140, 233, 302, 333, 675, 807
TscAI CASTG 2 cut(s) 700, 778
TseFI GTSAC 1 cut(s) 12
TseI GCWGC 2 cut(s) 710, 713
Tsp45I GTSAC 1 cut(s) 12
TspDTI ATGAA 4 cut(s) 29, 138, 169, 464
TspGWI ACGGA 2 cut(s) 351, 360
TspRI CASTG 2 cut(s) 700, 778
Van91I CCANNNNNTGG 1 cut(s) 572
VpaK11BI GGWCC 1 cut(s) 338
XapI RAATTY 1 cut(s) 489
XceI RCATGY 2 cut(s) 331, 628
XspI CTAG 4 cut(s) 53, 311, 516, 671
ZraI GACGTC 1 cut(s) 660
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.