Rroxscaffold_7G00186470
NAC Family

Probably inactive leucine-rich repeat receptor-like protein kinase At5g48380

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0003453
Physical Location & Seq
Forward (+)
25843272 .. 25844480
1209 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00186470.1

Sequence Viewer

Length: 654 bp
ATGTGCACTTATTGGTCTGCTCTACCGGATACGTGTTCTGGCAGTGAGAGTGACATCAACTGCTTGAAAAGTATAAAAGCATCACTTGAGGATCCTTTGGATATGTTAACCTCTTCATGGGATTTCAGCAACATCACCGAAGGTTCCATCTGTAGCTTTCTGGGAGTGGAGTGCTGGAATTCTAATGAGAACAAAGTGCTGAATATCAACCTTTCGGATTTGGGACTCAAGGGCCAATTTCCTCGCGGCATAACGAATTGTACAAGCTTAACAGGCTTAGATCTTTCGAGCAACAAGTTGAGCGGATCCATTCCTGCTGATATCAGTAGAATGACTAGAATCATTCCATTCAGTACGTTGCTTGCCCTCTCGTATAACAACTTCTCCGGGACAATCCCTGCCAGTATTTCCAACTGCACTTATCTGAATGTCCTCAAGCTTGATCACAACCAGCTATCCGGTCAGATTCCCGGAGAAATTGGCCAACTTTCCAGGCTTAAAACATTCAGTGTGGCCAACAATCTTCTGCATGGGCCAGTCCCAGACTTCGTTGTGGCTTCGGTTACAGCGGACAGTTACGCAAACAATCCCGGACTGTGCGGCGGTCCTCTGAAGAGTTGCCGGTCATCCGTGGAGATGTCGTTAAATACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8

Protein Analysis

217

Amino Acids

23.2

Weight (kDa)

4.91

Isoelectric Point (pI)

36.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 16 - 59 8.4e-06 Leucine rich repeat N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 303
AccII CGCG 1 cut(s) 246
AciI CCGC 5 cut(s) 246, 303, 569, 600, 603
AclWI GGATC 4 cut(s) 86, 99, 300, 313
AcoI YGGCCR 2 cut(s) 481, 513
AcsI RAATTY 1 cut(s) 178
AcuI CTGAAG 1 cut(s) 632
AfaI GTAC 2 cut(s) 262, 355
AfiI CCNNNNNNNGG 1 cut(s) 117
AflIII ACRYGT 1 cut(s) 32
AgsI TTSAA 1 cut(s) 67
AjnI CCWGG 1 cut(s) 491
AluBI AGCT 4 cut(s) 156, 267, 439, 454
AluI AGCT 4 cut(s) 156, 267, 439, 454
Alw21I GWGCWC 1 cut(s) 8
Alw44I GTGCAC 1 cut(s) 4
AlwI GGATC 4 cut(s) 86, 99, 300, 313
AoxI GGCC 4 cut(s) 232, 481, 513, 533
ApaLI GTGCAC 1 cut(s) 4
ApoI RAATTY 1 cut(s) 178
AspS9I GGNCC 3 cut(s) 232, 533, 605
AsuC2I CCSGG 3 cut(s) 388, 471, 591
AsuHPI GGTGA 1 cut(s) 127
AvaII GGWCC 1 cut(s) 605
BaeGI GKGCMC 1 cut(s) 8
BalI TGGCCA 2 cut(s) 483, 515
BamHI GGATCC 2 cut(s) 91, 305
BarI GAAGNNNNNNTAC 2 cut(s) 365, 397
Bbv12I GWGCWC 1 cut(s) 8
BccI CCATC 1 cut(s) 155
BciT130I CCWGG 1 cut(s) 493
BciVI GTATCC 1 cut(s) 22
BclI TGATCA 1 cut(s) 442
BcnI CCSGG 3 cut(s) 388, 471, 591
BfaI CTAG 1 cut(s) 336
BfmI CTRYAG 1 cut(s) 151
BfuI GTATCC 1 cut(s) 22
BglII AGATCT 1 cut(s) 280
BisI GCNGC 2 cut(s) 247, 601
BlsI GCNGC 2 cut(s) 248, 602
Bme1390I CCNGG 4 cut(s) 388, 471, 493, 591
Bme18I GGWCC 1 cut(s) 605
BmgT120I GGNCC 3 cut(s) 232, 533, 605
BmiI GGNNCC 3 cut(s) 93, 145, 307
BmrFI CCNGG 4 cut(s) 388, 471, 493, 591
BmsI GCATC 1 cut(s) 89
BpuEI CTTGAG 3 cut(s) 107, 212, 419
BpuMI CCSGG 3 cut(s) 388, 471, 591
BsaAI YACGTR 1 cut(s) 33
BsaJI CCNNGG 1 cut(s) 630
BsaWI WCCGGW 2 cut(s) 25, 458
BsaXI ACNNNNNCTCC 3 cut(s) 368, 398, 626
Bsc4I CCNNNNNNNGG 1 cut(s) 117
Bse118I RCCGGY 1 cut(s) 621
Bse1I ACTGG 2 cut(s) 402, 536
BseBI CCWGG 1 cut(s) 493
BseDI CCNNGG 1 cut(s) 630
BseGI GGATG 1 cut(s) 626
BseLI CCNNNNNNNGG 1 cut(s) 117
BseNI ACTGG 2 cut(s) 402, 536
BseSI GKGCMC 1 cut(s) 8
BsgI GTGCAG 1 cut(s) 400
Bsh1236I CGCG 1 cut(s) 246
BshFI GGCC 4 cut(s) 234, 483, 515, 535
BsiHKAI GWGCWC 1 cut(s) 8
BsiSI CCGG 6 cut(s) 26, 387, 459, 471, 591, 622
BslFI GGGAC 3 cut(s) 237, 403, 524
BslI CCNNNNNNNGG 1 cut(s) 117
BsmFI GGGAC 3 cut(s) 237, 403, 524
BsnI GGCC 4 cut(s) 234, 483, 515, 535
Bsp1286I GDGCHC 1 cut(s) 8
Bsp1407I TGTACA 1 cut(s) 260
Bsp143I GATC 4 cut(s) 91, 280, 305, 442
BspACI CCGC 5 cut(s) 246, 303, 569, 600, 603
BspANI GGCC 4 cut(s) 234, 483, 515, 535
BspFNI CGCG 1 cut(s) 246
BspLI GGNNCC 3 cut(s) 93, 145, 307
BspPI GGATC 4 cut(s) 86, 99, 300, 313
BsrBI CCGCTC 1 cut(s) 303
BsrFI RCCGGY 1 cut(s) 621
BsrGI TGTACA 1 cut(s) 260
BsrI ACTGG 2 cut(s) 402, 536
BssAI RCCGGY 1 cut(s) 621
BssECI CCNNGG 1 cut(s) 630
BssMI GATC 4 cut(s) 91, 280, 305, 442
Bst2UI CCWGG 1 cut(s) 493
Bst4CI ACNGT 2 cut(s) 575, 597
Bst6I CTCTTC 2 cut(s) 118, 608
BstAUI TGTACA 1 cut(s) 260
BstBAI YACGTR 1 cut(s) 33
BstC8I GCNNGC 1 cut(s) 363
BstDEI CTNAG 1 cut(s) 277
BstDSI CCRYGG 1 cut(s) 630
BstF5I GGATG 1 cut(s) 626
BstFNI CGCG 1 cut(s) 246
BstKTI GATC 4 cut(s) 94, 283, 308, 445
BstMBI GATC 4 cut(s) 91, 280, 305, 442
BstMWI GCNNNNNNNGC 1 cut(s) 273
BstNI CCWGG 1 cut(s) 493
BstSCI CCNGG 4 cut(s) 386, 469, 491, 589
BstSFI CTRYAG 1 cut(s) 151
BstSLI GKGCMC 1 cut(s) 8
BstUI CGCG 1 cut(s) 246
BstX2I RGATCY 3 cut(s) 91, 280, 305
BstYI RGATCY 3 cut(s) 91, 280, 305
BsuI GTATCC 1 cut(s) 22
BsuRI GGCC 4 cut(s) 234, 483, 515, 535
BtgI CCRYGG 1 cut(s) 630
BtsCI GGATG 1 cut(s) 626
BtsI GCAGTG 1 cut(s) 49
BtsIMutI CAGTG 2 cut(s) 49, 514
Cac8I GCNNGC 1 cut(s) 363
Cfr10I RCCGGY 1 cut(s) 621
Cfr13I GGNCC 3 cut(s) 232, 533, 605
Csp6I GTAC 2 cut(s) 261, 354
CviAII CATG 2 cut(s) 117, 530
CviQI GTAC 2 cut(s) 261, 354
DdeI CTNAG 1 cut(s) 277
DpnI GATC 4 cut(s) 93, 282, 307, 444
DpnII GATC 4 cut(s) 91, 280, 305, 442
EaeI YGGCCR 2 cut(s) 481, 513
Eam1104I CTCTTC 2 cut(s) 118, 608
EarI CTCTTC 2 cut(s) 118, 608
Eco32I GATATC 1 cut(s) 322
Eco47I GGWCC 1 cut(s) 605
Eco57I CTGAAG 1 cut(s) 632
EcoRI GAATTC 1 cut(s) 178
EcoRII CCWGG 1 cut(s) 491
EcoRV GATATC 1 cut(s) 322
FaeI CATG 2 cut(s) 120, 533
FaiI YATR 6 cut(s) 74, 104, 118, 251, 375, 531
FalI AAGNNNNNCTT 2 cut(s) 69, 101
FaqI GGGAC 3 cut(s) 237, 403, 524
FatI CATG 2 cut(s) 116, 529
FbaI TGATCA 1 cut(s) 442
Fnu4HI GCNGC 2 cut(s) 247, 601
FokI GGATG 1 cut(s) 613
Fsp4HI GCNGC 2 cut(s) 247, 601
FspBI CTAG 1 cut(s) 336
GluI GCNGC 2 cut(s) 247, 601
HaeIII GGCC 4 cut(s) 234, 483, 515, 535
HapII CCGG 6 cut(s) 26, 387, 459, 471, 591, 622
Hin1II CATG 2 cut(s) 120, 533
HincII GTYRAC 1 cut(s) 108
HindII GTYRAC 1 cut(s) 108
HindIII AAGCTT 2 cut(s) 265, 437
HinfI GANTC 3 cut(s) 225, 339, 466
HpaI GTTAAC 1 cut(s) 108
HpaII CCGG 6 cut(s) 26, 387, 459, 471, 591, 622
HphI GGTGA 1 cut(s) 127
Hpy166II GTNNAC 2 cut(s) 6, 108
Hpy188I TCNGA 4 cut(s) 217, 426, 465, 612
Hpy8I GTNNAC 2 cut(s) 6, 108
HpyAV CCTTC 1 cut(s) 134
HpyCH4III ACNGT 2 cut(s) 575, 597
HpyCH4IV ACGT 2 cut(s) 32, 356
HpyCH4V TGCA 3 cut(s) 6, 417, 529
HpyF10VI GCNNNNNNNGC 1 cut(s) 273
HpyF3I CTNAG 1 cut(s) 277
HpySE526I ACGT 2 cut(s) 32, 356
Hsp92II CATG 2 cut(s) 120, 533
Ksp22I TGATCA 1 cut(s) 442
KspAI GTTAAC 1 cut(s) 108
Kzo9I GATC 4 cut(s) 91, 280, 305, 442
LweI GCATC 1 cut(s) 89
MaeI CTAG 1 cut(s) 336
MaeII ACGT 2 cut(s) 32, 356
MaeIII GTNAC 3 cut(s) 50, 562, 575
MalI GATC 4 cut(s) 93, 282, 307, 444
MbiI CCGCTC 1 cut(s) 303
MboI GATC 4 cut(s) 91, 280, 305, 442
MboII GAAGA 3 cut(s) 105, 515, 625
MflI RGATCY 3 cut(s) 91, 280, 305
MhlI GDGCHC 1 cut(s) 8
MlsI TGGCCA 2 cut(s) 483, 515
MluCI AATT 4 cut(s) 178, 236, 256, 477
MluNI TGGCCA 2 cut(s) 483, 515
MlyI GAGTC 1 cut(s) 219
MmeI TCCRAC 1 cut(s) 435
MnlI CCTC 6 cut(s) 82, 121, 252, 377, 443, 618
Mox20I TGGCCA 2 cut(s) 483, 515
MscI TGGCCA 2 cut(s) 483, 515
MseI TTAA 5 cut(s) 107, 269, 498, 644, 652
Msp20I TGGCCA 2 cut(s) 483, 515
MspA1I CMGCKG 1 cut(s) 569
MspI CCGG 6 cut(s) 26, 387, 459, 471, 591, 622
MspR9I CCNGG 4 cut(s) 388, 471, 493, 591
MvaI CCWGG 1 cut(s) 493
MvnI CGCG 1 cut(s) 246
MwoI GCNNNNNNNGC 1 cut(s) 273
NciI CCSGG 3 cut(s) 388, 471, 591
NdeII GATC 4 cut(s) 91, 280, 305, 442
NlaIII CATG 2 cut(s) 120, 533
NlaIV GGNNCC 3 cut(s) 93, 145, 307
NmuCI GTSAC 1 cut(s) 50
PfeI GAWTC 2 cut(s) 339, 466
PfoI TCCNGGA 3 cut(s) 386, 469, 589
PkrI GCNGC 2 cut(s) 248, 602
PleI GAGTC 1 cut(s) 219
PpsI GAGTC 1 cut(s) 219
Ppu21I YACGTR 1 cut(s) 33
Psp6I CCWGG 1 cut(s) 491
PspGI CCWGG 1 cut(s) 491
PspN4I GGNNCC 3 cut(s) 93, 145, 307
PspPI GGNCC 3 cut(s) 232, 533, 605
PsuI RGATCY 3 cut(s) 91, 280, 305
RsaI GTAC 2 cut(s) 262, 355
RsaNI GTAC 2 cut(s) 261, 354
SaqAI TTAA 5 cut(s) 107, 269, 498, 644, 652
SatI GCNGC 2 cut(s) 247, 601
Sau3AI GATC 4 cut(s) 91, 280, 305, 442
Sau96I GGNCC 3 cut(s) 232, 533, 605
SchI GAGTC 1 cut(s) 219
ScrFI CCNGG 4 cut(s) 388, 471, 493, 591
SduI GDGCHC 1 cut(s) 8
SetI ASST 9 cut(s) 35, 113, 145, 158, 213, 269, 359, 441, 456
SfaNI GCATC 1 cut(s) 89
SfcI CTRYAG 1 cut(s) 151
SinI GGWCC 1 cut(s) 605
SmlI CTYRAG 3 cut(s) 86, 227, 434
SmoI CTYRAG 3 cut(s) 86, 227, 434
Sse9I AATT 4 cut(s) 178, 236, 256, 477
SsiI CCGC 5 cut(s) 246, 303, 569, 600, 603
SspMI CTAG 1 cut(s) 336
StyD4I CCNGG 4 cut(s) 386, 469, 491, 589
TaaI ACNGT 2 cut(s) 575, 597
TaiI ACGT 2 cut(s) 35, 359
TaqI TCGA 1 cut(s) 287
TasI AATT 4 cut(s) 178, 236, 256, 477
TatI WGTACW 1 cut(s) 260
TauI GCSGC 2 cut(s) 249, 603
TfiI GAWTC 2 cut(s) 339, 466
Tru1I TTAA 5 cut(s) 107, 269, 498, 644, 652
Tru9I TTAA 5 cut(s) 107, 269, 498, 644, 652
TscAI CASTG 2 cut(s) 49, 514
TseFI GTSAC 1 cut(s) 50
Tsp45I GTSAC 1 cut(s) 50
TspDTI ATGAA 1 cut(s) 105
TspGWI ACGGA 1 cut(s) 619
TspRI CASTG 2 cut(s) 49, 514
VneI GTGCAC 1 cut(s) 4
VpaK11BI GGWCC 1 cut(s) 605
XapI RAATTY 1 cut(s) 178
XspI CTAG 1 cut(s) 336
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.