MD10G1147500.v1.1

regulation of response to stimulus

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0003453
Physical Location & Seq
Reverse (-)
23548690 .. 23549881
1192 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1147500.v1.1.491

Sequence Viewer

Length: 897 bp
ATGGCTTTCTTAATACGTGATACCTTGTTGAGCAAACAACATCTTGGCAACATTTTTATTTGTTGCGTCTTATTGCTAAGTTGTAGTTTCAGCTCTGCTGTTGAGAGTGATATCAACTGCCTCATAAGCATAAAAGCATCACTTCAGGACCCTTTAGGTTACTTAAACTCTTCATGGGATTTCAACAATAACACAGAAGGTTTCATCTGTACGTTTCTGGGAATCGAGTGTTGGCACCTTCACGAGAGCAAAGTTTTAAATATCAAGCTTTCGGGTTTGGGACTCCAGGGCCCGTTTCCTCATGGTGTAGCGAATTGCACAAGCTTATCCGGATTAGATCTTTCAAGCAACAAGCTCAGTGGACCTCTTCCTGAAGATATCGGTAGAATTATTTCGTTCATTACAACTCTTGATCTCTCTTCTAACAGCTTCTCAGGGCAAATCCCAACAAATATTACCAATTGTAGCTATCTGAATGTGCTTAAGCTTGACAGCAACCAGTTCACCGGTAACATCCCTCTAGGGATTGGCCAGCTCGGTAGGATGAAAACATTTAGCGTGGCAAACAATCAGCTTTCTGGCCCAGTCCCAGAATTCGGTAACAATAGTGGAATTACAGCAGAAAGTTACGCAAACAATGCTGGACTTTGTGGGAAGCTTCTAAAGCCTTGTCGATCGTCTCAAGTGAAGTCCACTAGTACTCTAGTTAAGCTTCTCGGGGAAATTTCCCCCGAGTCTTCGAATTGTAGCAATCTGAATGTGCTTAAGCTTGAAAACAATGAGTTCTCGGGTGAAATTCCTGCACAACTTGCCTCGCTCAAAAGGCTTAATACATTTAGTGTGGCCAACAATCGTCTATCTGGTCCAATCCCAAATTCCGGTGGTTATGTGCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2

Protein Analysis

299

Amino Acids

31.87

Weight (kDa)

6.41

Isoelectric Point (pI)

39.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 35 - 78 8.4e-07 Leucine rich repeat N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 234
AccIII TCCGGA 1 cut(s) 329
AcoI YGGCCR 2 cut(s) 529, 843
AcsI RAATTY 4 cut(s) 593, 723, 795, 874
AcuI CTGAAG 2 cut(s) 128, 393
AfaI GTAC 2 cut(s) 211, 700
AfiI CCNNNNNNNGG 2 cut(s) 596, 878
AflII CTTAAG 2 cut(s) 482, 764
AgeI ACCGGT 1 cut(s) 506
AgsI TTSAA 3 cut(s) 184, 345, 773
AhlI ACTAGT 1 cut(s) 695
AjnI CCWGG 1 cut(s) 285
AjuI GAANNNNNNNTTGG 4 cut(s) 214, 246, 859, 891
Alw26I GTCTC 1 cut(s) 684
Ama87I CYCGRG 3 cut(s) 716, 731, 787
Aor13HI TCCGGA 1 cut(s) 329
AoxI GGCC 4 cut(s) 289, 529, 580, 843
ApaI GGGCCC 1 cut(s) 293
ApoI RAATTY 4 cut(s) 593, 723, 795, 874
AsiGI ACCGGT 1 cut(s) 506
Asp700I GAANNNNTTC 1 cut(s) 391
AspS9I GGNCC 6 cut(s) 148, 289, 290, 362, 581, 863
AsuHPI GGTGA 2 cut(s) 496, 803
AsuII TTCGAA 1 cut(s) 740
AvaI CYCGRG 3 cut(s) 716, 731, 787
AvaII GGWCC 3 cut(s) 148, 362, 863
BaeGI GKGCMC 1 cut(s) 293
BalI TGGCCA 2 cut(s) 531, 845
BanI GGYRCC 1 cut(s) 234
BanII GRGCYC 1 cut(s) 293
BauI CACGAG 1 cut(s) 242
BbsI GAAGAC 1 cut(s) 729
BciT130I CCWGG 1 cut(s) 287
BcoDI GTCTC 1 cut(s) 684
BcuI ACTAGT 1 cut(s) 695
BfaI CTAG 3 cut(s) 521, 696, 704
BfrI CTTAAG 2 cut(s) 482, 764
BglII AGATCT 1 cut(s) 337
BmcAI AGTACT 1 cut(s) 700
Bme1390I CCNGG 1 cut(s) 287
Bme18I GGWCC 3 cut(s) 148, 362, 863
BmeT110I CYCGRG 3 cut(s) 716, 731, 787
BmgT120I GGNCC 6 cut(s) 148, 289, 290, 362, 581, 863
BmiI GGNNCC 3 cut(s) 150, 236, 291
BmrFI CCNGG 1 cut(s) 287
BmrI ACTGGG 1 cut(s) 578
BmsI GCATC 1 cut(s) 146
BmuI ACTGGG 1 cut(s) 578
BpiI GAAGAC 1 cut(s) 729
BpmI CTGGAG 1 cut(s) 269
Bpu14I TTCGAA 1 cut(s) 740
BpuEI CTTGAG 1 cut(s) 666
BsaAI YACGTR 1 cut(s) 17
BsaJI CCNNGG 1 cut(s) 286
BsaWI WCCGGW 3 cut(s) 329, 506, 878
Bsc4I CCNNNNNNNGG 2 cut(s) 596, 878
Bse118I RCCGGY 1 cut(s) 506
Bse1I ACTGG 2 cut(s) 499, 584
BseAI TCCGGA 1 cut(s) 329
BseBI CCWGG 1 cut(s) 287
BseDI CCNNGG 1 cut(s) 286
BseGI GGATG 2 cut(s) 513, 549
BseLI CCNNNNNNNGG 2 cut(s) 596, 878
BseMII CTCAG 2 cut(s) 370, 447
BseNI ACTGG 2 cut(s) 499, 584
BseSI GKGCMC 1 cut(s) 293
BsgI GTGCAG 1 cut(s) 786
Bsh1285I CGRYCG 1 cut(s) 677
BshFI GGCC 4 cut(s) 291, 531, 582, 845
BshNI GGYRCC 1 cut(s) 234
BshTI ACCGGT 1 cut(s) 506
BsiEI CGRYCG 1 cut(s) 677
BsiHKCI CYCGRG 3 cut(s) 716, 731, 787
BsiSI CCGG 3 cut(s) 330, 507, 879
BslFI GGGAC 2 cut(s) 294, 572
BslI CCNNNNNNNGG 2 cut(s) 596, 878
BsmAI GTCTC 1 cut(s) 684
BsmBI CGTCTC 1 cut(s) 684
BsmFI GGGAC 2 cut(s) 294, 572
BsnI GGCC 4 cut(s) 291, 531, 582, 845
BsoBI CYCGRG 3 cut(s) 716, 731, 787
Bsp119I TTCGAA 1 cut(s) 740
Bsp120I GGGCCC 1 cut(s) 289
Bsp1286I GDGCHC 1 cut(s) 293
Bsp13I TCCGGA 1 cut(s) 329
Bsp143I GATC 3 cut(s) 337, 412, 674
BspANI GGCC 4 cut(s) 291, 531, 582, 845
BspCNI CTCAG 2 cut(s) 369, 446
BspEI TCCGGA 1 cut(s) 329
BspLI GGNNCC 3 cut(s) 150, 236, 291
BspT104I TTCGAA 1 cut(s) 740
BspT107I GGYRCC 1 cut(s) 234
BspTI CTTAAG 2 cut(s) 482, 764
BsrFI RCCGGY 1 cut(s) 506
BsrI ACTGG 2 cut(s) 499, 584
BssAI RCCGGY 1 cut(s) 506
BssECI CCNNGG 1 cut(s) 286
BssMI GATC 3 cut(s) 337, 412, 674
BssSI CACGAG 1 cut(s) 242
Bst2BI CACGAG 1 cut(s) 242
Bst2UI CCWGG 1 cut(s) 287
Bst6I CTCTTC 3 cut(s) 175, 372, 424
BstAFI CTTAAG 2 cut(s) 482, 764
BstAPI GCANNNNNTGC 2 cut(s) 638, 809
BstBAI YACGTR 1 cut(s) 17
BstBI TTCGAA 1 cut(s) 740
BstC8I GCNNGC 1 cut(s) 533
BstDEI CTNAG 3 cut(s) 77, 356, 433
BstF5I GGATG 2 cut(s) 513, 549
BstKTI GATC 3 cut(s) 340, 415, 677
BstMAI GTCTC 1 cut(s) 684
BstMBI GATC 3 cut(s) 337, 412, 674
BstMCI CGRYCG 1 cut(s) 677
BstMWI GCNNNNNNNGC 5 cut(s) 126, 638, 664, 809, 823
BstNI CCWGG 1 cut(s) 287
BstSCI CCNGG 1 cut(s) 285
BstSLI GKGCMC 1 cut(s) 293
BstV2I GAAGAC 1 cut(s) 729
BstX2I RGATCY 1 cut(s) 337
BstYI RGATCY 1 cut(s) 337
BsuRI GGCC 4 cut(s) 291, 531, 582, 845
BtsCI GGATG 2 cut(s) 513, 549
BtsIMutI CAGTG 1 cut(s) 364
Cac8I GCNNGC 1 cut(s) 533
Cfr10I RCCGGY 1 cut(s) 506
Cfr13I GGNCC 6 cut(s) 148, 289, 290, 362, 581, 863
CseI GACGC 1 cut(s) 55
Csp6I GTAC 2 cut(s) 210, 699
CspAI ACCGGT 1 cut(s) 506
CspCI CAANNNNNGTGG 2 cut(s) 340, 375
CviAII CATG 2 cut(s) 174, 302
CviQI GTAC 2 cut(s) 210, 699
DdeI CTNAG 3 cut(s) 77, 356, 433
DpnI GATC 3 cut(s) 339, 414, 676
DpnII GATC 3 cut(s) 337, 412, 674
DraI TTTAAA 1 cut(s) 258
EaeI YGGCCR 2 cut(s) 529, 843
Eam1104I CTCTTC 3 cut(s) 175, 372, 424
EarI CTCTTC 3 cut(s) 175, 372, 424
Eco24I GRGCYC 1 cut(s) 293
Eco32I GATATC 2 cut(s) 112, 379
Eco47I GGWCC 3 cut(s) 148, 362, 863
Eco57I CTGAAG 2 cut(s) 128, 393
Eco88I CYCGRG 3 cut(s) 716, 731, 787
EcoO109I RGGNCCY 2 cut(s) 148, 289
EcoRI GAATTC 1 cut(s) 593
EcoRII CCWGG 1 cut(s) 285
EcoRV GATATC 2 cut(s) 112, 379
EcoT38I GRGCYC 1 cut(s) 293
Esp3I CGTCTC 1 cut(s) 684
FaeI CATG 2 cut(s) 177, 305
FaiI YATR 5 cut(s) 125, 131, 175, 303, 888
FalI AAGNNNNNCTT 2 cut(s) 126, 158
FaqI GGGAC 2 cut(s) 294, 572
FatI CATG 2 cut(s) 173, 301
FokI GGATG 2 cut(s) 500, 556
FriOI GRGCYC 1 cut(s) 293
FspBI CTAG 3 cut(s) 521, 696, 704
GsuI CTGGAG 1 cut(s) 269
HaeIII GGCC 4 cut(s) 291, 531, 582, 845
HapII CCGG 3 cut(s) 330, 507, 879
HgaI GACGC 1 cut(s) 55
Hin1II CATG 2 cut(s) 177, 305
HindIII AAGCTT 6 cut(s) 266, 322, 485, 656, 710, 767
HinfI GANTC 3 cut(s) 222, 282, 734
HpaII CCGG 3 cut(s) 330, 507, 879
HphI GGTGA 2 cut(s) 496, 803
Hpy166II GTNNAC 3 cut(s) 362, 504, 693
Hpy188I TCNGA 2 cut(s) 474, 756
Hpy188III TCNNGA 5 cut(s) 146, 242, 330, 371, 410
Hpy8I GTNNAC 3 cut(s) 362, 504, 693
HpyAV CCTTC 2 cut(s) 191, 248
HpyCH4IV ACGT 2 cut(s) 16, 212
HpyCH4V TGCA 3 cut(s) 318, 803, 892
HpyF10VI GCNNNNNNNGC 5 cut(s) 126, 638, 664, 809, 823
HpyF3I CTNAG 3 cut(s) 77, 356, 433
HpySE526I ACGT 2 cut(s) 16, 212
Hsp92II CATG 2 cut(s) 177, 305
Kpn2I TCCGGA 1 cut(s) 329
Kzo9I GATC 3 cut(s) 337, 412, 674
LweI GCATC 1 cut(s) 146
MaeI CTAG 3 cut(s) 521, 696, 704
MaeII ACGT 2 cut(s) 16, 212
MaeIII GTNAC 4 cut(s) 158, 509, 599, 626
MalI GATC 3 cut(s) 339, 414, 676
MboI GATC 3 cut(s) 337, 412, 674
MboII GAAGA 5 cut(s) 162, 359, 386, 411, 729
MfeI CAATTG 1 cut(s) 460
MflI RGATCY 1 cut(s) 337
MhlI GDGCHC 1 cut(s) 293
MlsI TGGCCA 2 cut(s) 531, 845
MluCI AATT 9 cut(s) 313, 387, 460, 593, 612, 723, 742, 795, 874
MluNI TGGCCA 2 cut(s) 531, 845
MlyI GAGTC 2 cut(s) 276, 743
MnlI CCTC 5 cut(s) 131, 309, 375, 528, 823
Mox20I TGGCCA 2 cut(s) 531, 845
MroI TCCGGA 1 cut(s) 329
MroXI GAANNNNTTC 1 cut(s) 391
MscI TGGCCA 2 cut(s) 531, 845
MseI TTAA 7 cut(s) 11, 164, 257, 483, 708, 765, 828
Msp20I TGGCCA 2 cut(s) 531, 845
MspCI CTTAAG 2 cut(s) 482, 764
MspI CCGG 3 cut(s) 330, 507, 879
MspR9I CCNGG 1 cut(s) 287
MunI CAATTG 1 cut(s) 460
MvaI CCWGG 1 cut(s) 287
MwoI GCNNNNNNNGC 5 cut(s) 126, 638, 664, 809, 823
NdeII GATC 3 cut(s) 337, 412, 674
NlaIII CATG 2 cut(s) 177, 305
NlaIV GGNNCC 3 cut(s) 150, 236, 291
NspV TTCGAA 1 cut(s) 740
PdmI GAANNNNTTC 1 cut(s) 391
PfeI GAWTC 1 cut(s) 222
PinAI ACCGGT 1 cut(s) 506
Ple19I CGATCG 1 cut(s) 677
PleI GAGTC 2 cut(s) 276, 742
PpsI GAGTC 2 cut(s) 276, 742
Ppu21I YACGTR 1 cut(s) 17
PpuMI RGGWCCY 1 cut(s) 148
Psp5II RGGWCCY 1 cut(s) 148
Psp6I CCWGG 1 cut(s) 285
PspGI CCWGG 1 cut(s) 285
PspN4I GGNNCC 3 cut(s) 150, 236, 291
PspOMI GGGCCC 1 cut(s) 289
PspPI GGNCC 6 cut(s) 148, 289, 290, 362, 581, 863
PspPPI RGGWCCY 1 cut(s) 148
PsuI RGATCY 1 cut(s) 337
PvuI CGATCG 1 cut(s) 677
RsaI GTAC 2 cut(s) 211, 700
RsaNI GTAC 2 cut(s) 210, 699
SaqAI TTAA 7 cut(s) 11, 164, 257, 483, 708, 765, 828
Sau3AI GATC 3 cut(s) 337, 412, 674
Sau96I GGNCC 6 cut(s) 148, 289, 290, 362, 581, 863
ScaI AGTACT 1 cut(s) 700
SchI GAGTC 2 cut(s) 276, 743
ScrFI CCNGG 1 cut(s) 287
SduI GDGCHC 1 cut(s) 293
SfaNI GCATC 1 cut(s) 146
SfuI TTCGAA 1 cut(s) 740
SinI GGWCC 3 cut(s) 148, 362, 863
SmlI CTYRAG 3 cut(s) 482, 681, 764
SmoI CTYRAG 3 cut(s) 482, 681, 764
SpeI ACTAGT 1 cut(s) 695
Sse9I AATT 9 cut(s) 313, 387, 460, 593, 612, 723, 742, 795, 874
SspI AATATT 1 cut(s) 454
SspMI CTAG 3 cut(s) 521, 696, 704
StyD4I CCNGG 1 cut(s) 285
TaiI ACGT 2 cut(s) 19, 215
TaqI TCGA 3 cut(s) 225, 673, 740
TasI AATT 9 cut(s) 313, 387, 460, 593, 612, 723, 742, 795, 874
TatI WGTACW 1 cut(s) 698
TfiI GAWTC 1 cut(s) 222
Tru1I TTAA 7 cut(s) 11, 164, 257, 483, 708, 765, 828
Tru9I TTAA 7 cut(s) 11, 164, 257, 483, 708, 765, 828
TscAI CASTG 1 cut(s) 364
TspDTI ATGAA 4 cut(s) 162, 193, 388, 560
TspRI CASTG 1 cut(s) 364
Vha464I CTTAAG 2 cut(s) 482, 764
VpaK11BI GGWCC 3 cut(s) 148, 362, 863
XapI RAATTY 4 cut(s) 593, 723, 795, 874
XmnI GAANNNNTTC 1 cut(s) 391
XspI CTAG 3 cut(s) 521, 696, 704
ZrmI AGTACT 1 cut(s) 700
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.