pycom10g12740
NAC Family

Probably inactive leucine-rich repeat receptor-like protein kinase At5g48380

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0003453
Physical Location & Seq
Reverse (-)
16258122 .. 16258958
837 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g12740.1

Sequence Viewer

Length: 837 bp
ATGGCTTTCTTAATACGTGATATCTTGTTGAGCAAACAACATCTTGGCAACATTTTTATTTGTTGCGTCTTATTGCTAAGTTGTAGTTTCAGCTCTGCTGTTGAGAGTGATATCAACTGCCTCAAAAGCATAAAAGCATCACTTCAGGACCCTTTAGGTTACTTAAACTCTTCATGGGATTTTAGCAACCACACCGAAGGCTTCATCTGCAAATTTCTTGGTATTGAGTGCTGGCACGCAAACGAGAGCAAAGTTTTGAGCATCAAGCTTTCGGATTTGGGACTCAAGGGCCCGTTTCCTCGTGGTGTAGCGAATTGCACAAGCTTATCTGGATTGGATCTTTCGAGCAACAAGCTCAGTGGACCTCTTCCTGAAGATCTTGGTAGAATCCTAGCGTTTATTACGATTCTTGATCTCTCATCGAACAGCTTCTCGGGGAAAATCCCCCGCAGTCTTCCGAAATGTAGCTGTCTGAATGTGCTTGAGCTTGAAAACAATGAGTTCTCGGGTGAAATTCCTGCACAACTTGGCCAGCTCACAAGGCTTGAAATATTTAGCGTGGCCAACAATCTTTTGTCTGGCCAAGTCCCAAACTTCGGTAGCCATACTGGCACTGCAATCACTGCAGAAAGTTACGCAAACAATGCTGGACTTTGTGGGAAGCCTCTGAAGCGTTGTCAATCGACTCTAGTTAAGGCTTTGGAGTCAAACAGTGTTGTTATTGCAGCAGCAGCTGGTTTTGTAATTGCTTTTCCTTTTTCTGTCTGTTTTTTCCTTCCAAGAGCTCCTAGTGTGGATCGCTTGCGGGTTTTCTATCGAGGATTTAAACTTTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8

Protein Analysis

279

Amino Acids

30.1

Weight (kDa)

8.24

Isoelectric Point (pI)

39.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 448, 805
AclWI GGATC 2 cut(s) 345, 804
AcoI YGGCCR 3 cut(s) 529, 561, 580
AcsI RAATTY 2 cut(s) 212, 513
AcuI CTGAAG 3 cut(s) 128, 393, 689
AfiI CCNNNNNNNGG 1 cut(s) 596
AgsI TTSAA 2 cut(s) 491, 548
Alw21I GWGCWC 1 cut(s) 787
AlwI GGATC 2 cut(s) 345, 804
AlwNI CAGNNNCTG 1 cut(s) 734
Ama87I CYCGRG 2 cut(s) 433, 505
AoxI GGCC 4 cut(s) 289, 529, 561, 580
ApaI GGGCCC 1 cut(s) 293
ApeKI GCWGC 3 cut(s) 725, 728, 731
ApoI RAATTY 2 cut(s) 212, 513
Asp700I GAANNNNTTC 1 cut(s) 428
AspS9I GGNCC 4 cut(s) 148, 289, 290, 362
AsuHPI GGTGA 1 cut(s) 521
AvaI CYCGRG 2 cut(s) 433, 505
AvaII GGWCC 2 cut(s) 148, 362
BaeGI GKGCMC 1 cut(s) 293
BalI TGGCCA 3 cut(s) 531, 563, 582
BanII GRGCYC 2 cut(s) 293, 787
BauI CACGAG 1 cut(s) 300
BbsI GAAGAC 1 cut(s) 446
Bbv12I GWGCWC 1 cut(s) 787
BbvI GCAGC 3 cut(s) 737, 740, 743
BfaI CTAG 3 cut(s) 392, 689, 789
BfmI CTRYAG 1 cut(s) 624
BglI GCCNNNNNGGC 1 cut(s) 609
BglII AGATCT 1 cut(s) 376
BisI GCNGC 3 cut(s) 726, 729, 732
BlsI GCNGC 3 cut(s) 727, 730, 733
Bme18I GGWCC 2 cut(s) 148, 362
BmeT110I CYCGRG 2 cut(s) 433, 505
BmgT120I GGNCC 4 cut(s) 148, 289, 290, 362
BmiI GGNNCC 2 cut(s) 150, 291
BmsI GCATC 2 cut(s) 146, 270
BpiI GAAGAC 1 cut(s) 446
BpuEI CTTGAG 2 cut(s) 269, 503
BsaAI YACGTR 1 cut(s) 17
Bsc4I CCNNNNNNNGG 1 cut(s) 596
Bse1I ACTGG 1 cut(s) 613
BseLI CCNNNNNNNGG 1 cut(s) 596
BseMII CTCAG 1 cut(s) 370
BseNI ACTGG 1 cut(s) 613
BseSI GKGCMC 1 cut(s) 293
BseXI GCAGC 3 cut(s) 737, 740, 743
BsgI GTGCAG 1 cut(s) 504
BshFI GGCC 4 cut(s) 291, 531, 563, 582
BsiHKAI GWGCWC 1 cut(s) 787
BsiHKCI CYCGRG 2 cut(s) 433, 505
BslFI GGGAC 2 cut(s) 294, 572
BslI CCNNNNNNNGG 1 cut(s) 596
BsmFI GGGAC 2 cut(s) 294, 572
BsnI GGCC 4 cut(s) 291, 531, 563, 582
BsoBI CYCGRG 2 cut(s) 433, 505
Bsp120I GGGCCC 1 cut(s) 289
Bsp1286I GDGCHC 2 cut(s) 293, 787
Bsp143I GATC 4 cut(s) 337, 376, 412, 796
BspACI CCGC 2 cut(s) 448, 805
BspANI GGCC 4 cut(s) 291, 531, 563, 582
BspCNI CTCAG 1 cut(s) 369
BspLI GGNNCC 2 cut(s) 150, 291
BspMAI CTGCAG 1 cut(s) 628
BspPI GGATC 2 cut(s) 345, 804
BsrI ACTGG 1 cut(s) 613
BssMI GATC 4 cut(s) 337, 376, 412, 796
BssSI CACGAG 1 cut(s) 300
Bst2BI CACGAG 1 cut(s) 300
Bst4CI ACNGT 1 cut(s) 713
Bst6I CTCTTC 2 cut(s) 175, 372
BstAPI GCANNNNNTGC 2 cut(s) 623, 644
BstBAI YACGTR 1 cut(s) 17
BstC8I GCNNGC 4 cut(s) 233, 237, 533, 803
BstDEI CTNAG 2 cut(s) 77, 356
BstKTI GATC 4 cut(s) 340, 379, 415, 799
BstMBI GATC 4 cut(s) 337, 376, 412, 796
BstMWI GCNNNNNNNGC 8 cut(s) 126, 207, 541, 609, 623, 644, 670, 731
BstSFI CTRYAG 1 cut(s) 624
BstSLI GKGCMC 1 cut(s) 293
BstV1I GCAGC 3 cut(s) 737, 740, 743
BstV2I GAAGAC 1 cut(s) 446
BstX2I RGATCY 2 cut(s) 337, 376
BstYI RGATCY 2 cut(s) 337, 376
BsuRI GGCC 4 cut(s) 291, 531, 563, 582
BtsI GCAGTG 2 cut(s) 612, 621
BtsIMutI CAGTG 4 cut(s) 364, 612, 621, 718
Cac8I GCNNGC 4 cut(s) 233, 237, 533, 803
CaiI CAGNNNCTG 1 cut(s) 734
Cfr13I GGNCC 4 cut(s) 148, 289, 290, 362
CseI GACGC 1 cut(s) 55
CspCI CAANNNNNGTGG 2 cut(s) 340, 375
CviAII CATG 1 cut(s) 174
DdeI CTNAG 2 cut(s) 77, 356
DpnI GATC 4 cut(s) 339, 378, 414, 798
DpnII GATC 4 cut(s) 337, 376, 412, 796
DraI TTTAAA 1 cut(s) 826
EaeI YGGCCR 3 cut(s) 529, 561, 580
Eam1104I CTCTTC 2 cut(s) 175, 372
EarI CTCTTC 2 cut(s) 175, 372
Ecl136II GAGCTC 1 cut(s) 785
Eco24I GRGCYC 2 cut(s) 293, 787
Eco32I GATATC 2 cut(s) 22, 112
Eco47I GGWCC 2 cut(s) 148, 362
Eco53kI GAGCTC 1 cut(s) 785
Eco57I CTGAAG 3 cut(s) 128, 393, 689
Eco88I CYCGRG 2 cut(s) 433, 505
EcoICRI GAGCTC 1 cut(s) 785
EcoO109I RGGNCCY 2 cut(s) 148, 289
EcoRV GATATC 2 cut(s) 22, 112
EcoT38I GRGCYC 2 cut(s) 293, 787
FaeI CATG 1 cut(s) 177
FaiI YATR 3 cut(s) 131, 175, 606
FalI AAGNNNNNCTT 2 cut(s) 126, 158
FaqI GGGAC 2 cut(s) 294, 572
FatI CATG 1 cut(s) 173
FauI CCCGC 2 cut(s) 455, 798
Fnu4HI GCNGC 3 cut(s) 726, 729, 732
FriOI GRGCYC 2 cut(s) 293, 787
Fsp4HI GCNGC 3 cut(s) 726, 729, 732
FspBI CTAG 3 cut(s) 392, 689, 789
GluI GCNGC 3 cut(s) 726, 729, 732
HaeIII GGCC 4 cut(s) 291, 531, 563, 582
HgaI GACGC 1 cut(s) 55
Hin1II CATG 1 cut(s) 177
HindIII AAGCTT 2 cut(s) 266, 322
HinfI GANTC 5 cut(s) 282, 387, 406, 685, 704
HphI GGTGA 1 cut(s) 521
Hpy166II GTNNAC 1 cut(s) 362
Hpy188I TCNGA 4 cut(s) 274, 459, 474, 669
Hpy188III TCNNGA 4 cut(s) 146, 330, 371, 410
Hpy8I GTNNAC 1 cut(s) 362
HpyAV CCTTC 2 cut(s) 191, 785
HpyCH4III ACNGT 1 cut(s) 713
HpyCH4IV ACGT 1 cut(s) 16
HpyCH4V TGCA 6 cut(s) 210, 318, 521, 617, 626, 725
HpyF10VI GCNNNNNNNGC 8 cut(s) 126, 207, 541, 609, 623, 644, 670, 731
HpyF3I CTNAG 2 cut(s) 77, 356
HpySE526I ACGT 1 cut(s) 16
Hsp92II CATG 1 cut(s) 177
Kzo9I GATC 4 cut(s) 337, 376, 412, 796
LmnI GCTCC 1 cut(s) 790
Lsp1109I GCAGC 3 cut(s) 737, 740, 743
LweI GCATC 2 cut(s) 146, 270
MaeI CTAG 3 cut(s) 392, 689, 789
MaeII ACGT 1 cut(s) 16
MaeIII GTNAC 2 cut(s) 158, 632
MalI GATC 4 cut(s) 339, 378, 414, 798
MboI GATC 4 cut(s) 337, 376, 412, 796
MboII GAAGA 4 cut(s) 162, 359, 386, 446
MflI RGATCY 2 cut(s) 337, 376
MhlI GDGCHC 2 cut(s) 293, 787
MlsI TGGCCA 3 cut(s) 531, 563, 582
MluCI AATT 4 cut(s) 212, 313, 513, 744
MluNI TGGCCA 3 cut(s) 531, 563, 582
MlyI GAGTC 3 cut(s) 276, 679, 713
MnlI CCTC 5 cut(s) 131, 309, 375, 675, 812
Mox20I TGGCCA 3 cut(s) 531, 563, 582
MroXI GAANNNNTTC 1 cut(s) 428
MscI TGGCCA 3 cut(s) 531, 563, 582
MseI TTAA 4 cut(s) 11, 164, 693, 825
Msp20I TGGCCA 3 cut(s) 531, 563, 582
MspA1I CMGCKG 1 cut(s) 734
MwoI GCNNNNNNNGC 8 cut(s) 126, 207, 541, 609, 623, 644, 670, 731
NdeII GATC 4 cut(s) 337, 376, 412, 796
NlaIII CATG 1 cut(s) 177
NlaIV GGNNCC 2 cut(s) 150, 291
PdmI GAANNNNTTC 1 cut(s) 428
PfeI GAWTC 2 cut(s) 387, 406
PkrI GCNGC 3 cut(s) 727, 730, 733
PleI GAGTC 3 cut(s) 276, 679, 712
PpsI GAGTC 3 cut(s) 276, 679, 712
Ppu21I YACGTR 1 cut(s) 17
PpuMI RGGWCCY 1 cut(s) 148
Psp124BI GAGCTC 1 cut(s) 787
Psp5II RGGWCCY 1 cut(s) 148
PspN4I GGNNCC 2 cut(s) 150, 291
PspOMI GGGCCC 1 cut(s) 289
PspPI GGNCC 4 cut(s) 148, 289, 290, 362
PspPPI RGGWCCY 1 cut(s) 148
PstI CTGCAG 1 cut(s) 628
PstNI CAGNNNCTG 1 cut(s) 734
PsuI RGATCY 2 cut(s) 337, 376
PvuII CAGCTG 1 cut(s) 734
SacI GAGCTC 1 cut(s) 787
SaqAI TTAA 4 cut(s) 11, 164, 693, 825
SatI GCNGC 3 cut(s) 726, 729, 732
Sau3AI GATC 4 cut(s) 337, 376, 412, 796
Sau96I GGNCC 4 cut(s) 148, 289, 290, 362
SchI GAGTC 3 cut(s) 276, 679, 713
SduI GDGCHC 2 cut(s) 293, 787
SfaNI GCATC 2 cut(s) 146, 270
SfcI CTRYAG 1 cut(s) 624
SinI GGWCC 2 cut(s) 148, 362
SmlI CTYRAG 2 cut(s) 284, 482
SmoI CTYRAG 2 cut(s) 284, 482
Sse9I AATT 4 cut(s) 212, 313, 513, 744
SsiI CCGC 2 cut(s) 448, 805
SspI AATATT 1 cut(s) 552
SspMI CTAG 3 cut(s) 392, 689, 789
SstI GAGCTC 1 cut(s) 787
TaaI ACNGT 1 cut(s) 713
TaiI ACGT 1 cut(s) 19
TaqI TCGA 4 cut(s) 344, 422, 683, 817
TasI AATT 4 cut(s) 212, 313, 513, 744
TfiI GAWTC 2 cut(s) 387, 406
Tru1I TTAA 4 cut(s) 11, 164, 693, 825
Tru9I TTAA 4 cut(s) 11, 164, 693, 825
TscAI CASTG 4 cut(s) 364, 619, 628, 718
TseI GCWGC 3 cut(s) 725, 728, 731
TspDTI ATGAA 2 cut(s) 162, 193
TspRI CASTG 4 cut(s) 364, 619, 628, 718
VpaK11BI GGWCC 2 cut(s) 148, 362
XapI RAATTY 2 cut(s) 212, 513
XmnI GAANNNNTTC 1 cut(s) 428
XspI CTAG 3 cut(s) 392, 689, 789
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.