Prupe.8G186500_v2.0.a1
NAC Family

Probably inactive leucine-rich repeat receptor-like protein kinase At5g48380

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0003453
Physical Location & Seq
Reverse (-)
18390621 .. 18391868
1248 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G186500.1

Sequence Viewer

Length: 759 bp
ATTTATATTGGCAGTGTCCTCTTGTTGTTGCTGCTTAGTAATTGTAGCTTCAGCTTTGGTGTTGAGAGCGACATCAACTGCTTGAAAAGTATAAAAGCATCACTTGAGGACACTTTGGGGTACATAAACTCTTCATGGGATTTCAGCAACAACACTGATGGCTTCATCTGCAACTTTCTTGGAATCGAGTGTTGGCACCCTCAAGAGAGCAAGGTTCTGAACATCAAGCTTTCGGACTTGGGACTAAAAGGTCAGTTCCCTCGGGGCGTAGAAAATTGTACAAGCTTAACAGGTTTAGATCTTTCAAGCAACATGCTCAGTGGACATCTTCCTAATGATATCGATGAAGTTCTTTTGTTTGTTACGTCTCTTGATCTCTCTTCCAACAGCTTCTCAGGGCTGATCCCCGCGACACTTTCCAATTGCAGCTATATGAATGTGCTCAAGCTTGATAACAACCAGTTCTCGGGTAACATTCCGGCAGAAGTTAACCAGCTAACTAGGCTTAAAACTTTTAGTGTGGCCAACAATGCTCTGTCTGGCGAAGTCCCATCATTCAATACTACATCAATTACAGCGGACAGTTATGCAAACAATCCCGGACTCTGTGGCCACCCGCTCAAGTCTTGCCAAAAGAAGTCCAAGAGTGTGGTTAGGGTTTTCATGTCCAGGAATGGTGTTGTTATTGTGGCAGCAGCTGGTTTTGGTGTTGGTTTTGCCCTTTATTTTGAGATAAACTCCAATAGCTCAATAGCTCAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8

Protein Analysis

253

Amino Acids

27.21

Weight (kDa)

5.14

Isoelectric Point (pI)

28.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 249
AccB1I GGYRCC 1 cut(s) 195
AccBSI CCGCTC 1 cut(s) 619
AccII CGCG 1 cut(s) 410
AciI CCGC 3 cut(s) 408, 578, 617
AclWI GGATC 1 cut(s) 397
AcoI YGGCCR 2 cut(s) 522, 610
AcuI CTGAAG 1 cut(s) 34
AfaI GTAC 2 cut(s) 122, 280
AfiI CCNNNNNNNGG 1 cut(s) 466
AgsI TTSAA 3 cut(s) 85, 306, 559
AjnI CCWGG 1 cut(s) 668
AjuI GAANNNNNNNTTGG 2 cut(s) 175, 207
Alw21I GWGCWC 1 cut(s) 444
Alw26I GTCTC 1 cut(s) 372
AlwI GGATC 1 cut(s) 397
AlwNI CAGNNNCTG 1 cut(s) 698
Ama87I CYCGRG 2 cut(s) 261, 466
AoxI GGCC 2 cut(s) 522, 610
ApeKI GCWGC 4 cut(s) 31, 426, 692, 695
AsuC2I CCSGG 1 cut(s) 600
AvaI CYCGRG 2 cut(s) 261, 466
BalI TGGCCA 2 cut(s) 524, 612
BanI GGYRCC 1 cut(s) 195
Bbv12I GWGCWC 1 cut(s) 444
BbvI GCAGC 4 cut(s) 18, 438, 704, 707
BccI CCATC 2 cut(s) 152, 559
BciT130I CCWGG 1 cut(s) 670
BcnI CCSGG 1 cut(s) 600
BcoDI GTCTC 1 cut(s) 372
BfaI CTAG 1 cut(s) 501
BglII AGATCT 1 cut(s) 298
BisI GCNGC 4 cut(s) 32, 427, 693, 696
BlsI GCNGC 4 cut(s) 33, 428, 694, 697
Bme1390I CCNGG 2 cut(s) 600, 670
BmeT110I CYCGRG 2 cut(s) 261, 466
BmiI GGNNCC 1 cut(s) 197
BmrFI CCNGG 2 cut(s) 600, 670
BmsI GCATC 1 cut(s) 107
BplI GAGNNNNNCTC 2 cut(s) 722, 754
BpuEI CTTGAG 4 cut(s) 125, 186, 428, 605
BpuMI CCSGG 1 cut(s) 600
Bsa29I ATCGAT 1 cut(s) 342
BsaJI CCNNGG 1 cut(s) 260
Bsc4I CCNNNNNNNGG 1 cut(s) 466
Bse1I ACTGG 1 cut(s) 460
BseBI CCWGG 1 cut(s) 670
BseCI ATCGAT 1 cut(s) 342
BseDI CCNNGG 1 cut(s) 260
BseLI CCNNNNNNNGG 1 cut(s) 466
BseMII CTCAG 2 cut(s) 331, 408
BseNI ACTGG 1 cut(s) 460
BseXI GCAGC 4 cut(s) 18, 438, 704, 707
Bsh1236I CGCG 1 cut(s) 410
BshFI GGCC 2 cut(s) 524, 612
BshNI GGYRCC 1 cut(s) 195
BshVI ATCGAT 1 cut(s) 342
BsiHKAI GWGCWC 1 cut(s) 444
BsiHKCI CYCGRG 2 cut(s) 261, 466
BsiSI CCGG 2 cut(s) 479, 600
BslFI GGGAC 2 cut(s) 255, 533
BslI CCNNNNNNNGG 1 cut(s) 466
BsmAI GTCTC 1 cut(s) 372
BsmBI CGTCTC 1 cut(s) 372
BsmFI GGGAC 2 cut(s) 255, 533
BsnI GGCC 2 cut(s) 524, 612
BsoBI CYCGRG 2 cut(s) 261, 466
Bsp1286I GDGCHC 1 cut(s) 444
Bsp1407I TGTACA 1 cut(s) 278
Bsp143I GATC 3 cut(s) 298, 373, 402
BspACI CCGC 3 cut(s) 408, 578, 617
BspANI GGCC 2 cut(s) 524, 612
BspCNI CTCAG 2 cut(s) 330, 407
BspDI ATCGAT 1 cut(s) 342
BspFNI CGCG 1 cut(s) 410
BspLI GGNNCC 1 cut(s) 197
BspPI GGATC 1 cut(s) 397
BspT107I GGYRCC 1 cut(s) 195
BsrBI CCGCTC 1 cut(s) 619
BsrGI TGTACA 1 cut(s) 278
BsrI ACTGG 1 cut(s) 460
BssECI CCNNGG 1 cut(s) 260
BssMI GATC 3 cut(s) 298, 373, 402
Bst2UI CCWGG 1 cut(s) 670
Bst4CI ACNGT 1 cut(s) 584
Bst6I CTCTTC 2 cut(s) 136, 385
BstAUI TGTACA 1 cut(s) 278
BstDEI CTNAG 3 cut(s) 35, 317, 394
BstFNI CGCG 1 cut(s) 410
BstKTI GATC 3 cut(s) 301, 376, 405
BstMAI GTCTC 1 cut(s) 372
BstMBI GATC 3 cut(s) 298, 373, 402
BstMWI GCNNNNNNNGC 3 cut(s) 168, 502, 530
BstNI CCWGG 1 cut(s) 670
BstNSI RCATGY 1 cut(s) 316
BstSCI CCNGG 2 cut(s) 598, 668
BstUI CGCG 1 cut(s) 410
BstV1I GCAGC 4 cut(s) 18, 438, 704, 707
BstX2I RGATCY 1 cut(s) 298
BstXI CCANNNNNNTGG 1 cut(s) 649
BstYI RGATCY 1 cut(s) 298
Bsu15I ATCGAT 1 cut(s) 342
BsuRI GGCC 2 cut(s) 524, 612
BsuTUI ATCGAT 1 cut(s) 342
BtsI GCAGTG 1 cut(s) 19
BtsIMutI CAGTG 3 cut(s) 19, 153, 325
CaiI CAGNNNCTG 1 cut(s) 698
ClaI ATCGAT 1 cut(s) 342
Csp6I GTAC 2 cut(s) 121, 279
CviAII CATG 3 cut(s) 135, 313, 664
CviQI GTAC 2 cut(s) 121, 279
DdeI CTNAG 3 cut(s) 35, 317, 394
DpnI GATC 3 cut(s) 300, 375, 404
DpnII GATC 3 cut(s) 298, 373, 402
DrdI GACNNNNNNGTC 1 cut(s) 249
DseDI GACNNNNNNGTC 1 cut(s) 249
EaeI YGGCCR 2 cut(s) 522, 610
Eam1104I CTCTTC 2 cut(s) 136, 385
EarI CTCTTC 2 cut(s) 136, 385
Eco32I GATATC 1 cut(s) 340
Eco57I CTGAAG 1 cut(s) 34
Eco88I CYCGRG 2 cut(s) 261, 466
EcoRII CCWGG 1 cut(s) 668
EcoRV GATATC 1 cut(s) 340
Esp3I CGTCTC 1 cut(s) 372
FaeI CATG 3 cut(s) 138, 316, 667
FaiI YATR 9 cut(s) 6, 92, 125, 136, 314, 432, 434, 588, 665
FalI AAGNNNNNCTT 2 cut(s) 87, 119
FaqI GGGAC 2 cut(s) 255, 533
FatI CATG 3 cut(s) 134, 312, 663
FauI CCCGC 2 cut(s) 415, 624
Fnu4HI GCNGC 4 cut(s) 32, 427, 693, 696
Fsp4HI GCNGC 4 cut(s) 32, 427, 693, 696
FspBI CTAG 1 cut(s) 501
GluI GCNGC 4 cut(s) 32, 427, 693, 696
HaeIII GGCC 2 cut(s) 524, 612
HapII CCGG 2 cut(s) 479, 600
Hin1II CATG 3 cut(s) 138, 316, 667
HincII GTYRAC 1 cut(s) 490
HindII GTYRAC 1 cut(s) 490
HindIII AAGCTT 3 cut(s) 227, 283, 446
HinfI GANTC 2 cut(s) 183, 603
HpaI GTTAAC 1 cut(s) 490
HpaII CCGG 2 cut(s) 479, 600
Hpy166II GTNNAC 2 cut(s) 323, 490
Hpy188I TCNGA 2 cut(s) 219, 235
Hpy188III TCNNGA 2 cut(s) 203, 371
Hpy8I GTNNAC 2 cut(s) 323, 490
HpyCH4III ACNGT 1 cut(s) 584
HpyCH4IV ACGT 1 cut(s) 365
HpyCH4V TGCA 3 cut(s) 171, 426, 590
HpyF10VI GCNNNNNNNGC 3 cut(s) 168, 502, 530
HpyF3I CTNAG 3 cut(s) 35, 317, 394
HpySE526I ACGT 1 cut(s) 365
Hsp92II CATG 3 cut(s) 138, 316, 667
KspAI GTTAAC 1 cut(s) 490
Kzo9I GATC 3 cut(s) 298, 373, 402
Lsp1109I GCAGC 4 cut(s) 18, 438, 704, 707
LweI GCATC 1 cut(s) 107
MaeI CTAG 1 cut(s) 501
MaeII ACGT 1 cut(s) 365
MaeIII GTNAC 2 cut(s) 361, 470
MalI GATC 3 cut(s) 300, 375, 404
MbiI CCGCTC 1 cut(s) 619
MboI GATC 3 cut(s) 298, 373, 402
MboII GAAGA 3 cut(s) 123, 320, 372
MfeI CAATTG 1 cut(s) 421
MflI RGATCY 1 cut(s) 298
MhlI GDGCHC 1 cut(s) 444
MlsI TGGCCA 2 cut(s) 524, 612
MluCI AATT 4 cut(s) 40, 274, 421, 570
MluNI TGGCCA 2 cut(s) 524, 612
MlyI GAGTC 1 cut(s) 597
MmeI TCCRAC 1 cut(s) 408
MnlI CCTC 4 cut(s) 29, 100, 210, 270
Mox20I TGGCCA 2 cut(s) 524, 612
MscI TGGCCA 2 cut(s) 524, 612
MseI TTAA 3 cut(s) 287, 489, 507
Msp20I TGGCCA 2 cut(s) 524, 612
MspA1I CMGCKG 2 cut(s) 578, 698
MspI CCGG 2 cut(s) 479, 600
MspR9I CCNGG 2 cut(s) 600, 670
MunI CAATTG 1 cut(s) 421
MvaI CCWGG 1 cut(s) 670
MvnI CGCG 1 cut(s) 410
MwoI GCNNNNNNNGC 3 cut(s) 168, 502, 530
NciI CCSGG 1 cut(s) 600
NdeII GATC 3 cut(s) 298, 373, 402
NlaIII CATG 3 cut(s) 138, 316, 667
NlaIV GGNNCC 1 cut(s) 197
NspI RCATGY 1 cut(s) 316
PfeI GAWTC 1 cut(s) 183
PfoI TCCNGGA 2 cut(s) 598, 668
PkrI GCNGC 4 cut(s) 33, 428, 694, 697
PleI GAGTC 1 cut(s) 597
PpsI GAGTC 1 cut(s) 597
Psp6I CCWGG 1 cut(s) 668
PspGI CCWGG 1 cut(s) 668
PspN4I GGNNCC 1 cut(s) 197
PstNI CAGNNNCTG 1 cut(s) 698
PsuI RGATCY 1 cut(s) 298
PvuII CAGCTG 1 cut(s) 698
RsaI GTAC 2 cut(s) 122, 280
RsaNI GTAC 2 cut(s) 121, 279
SaqAI TTAA 3 cut(s) 287, 489, 507
SatI GCNGC 4 cut(s) 32, 427, 693, 696
Sau3AI GATC 3 cut(s) 298, 373, 402
SchI GAGTC 1 cut(s) 597
ScrFI CCNGG 2 cut(s) 600, 670
SduI GDGCHC 1 cut(s) 444
SfaNI GCATC 1 cut(s) 107
SmlI CTYRAG 4 cut(s) 104, 201, 443, 620
SmoI CTYRAG 4 cut(s) 104, 201, 443, 620
Sse9I AATT 4 cut(s) 40, 274, 421, 570
SsiI CCGC 3 cut(s) 408, 578, 617
SspMI CTAG 1 cut(s) 501
StyD4I CCNGG 2 cut(s) 598, 668
TaaI ACNGT 1 cut(s) 584
TaiI ACGT 1 cut(s) 368
TaqI TCGA 2 cut(s) 186, 342
TasI AATT 4 cut(s) 40, 274, 421, 570
TatI WGTACW 1 cut(s) 278
TfiI GAWTC 1 cut(s) 183
Tru1I TTAA 3 cut(s) 287, 489, 507
Tru9I TTAA 3 cut(s) 287, 489, 507
TscAI CASTG 3 cut(s) 19, 160, 325
TseI GCWGC 4 cut(s) 31, 426, 692, 695
TspDTI ATGAA 5 cut(s) 123, 154, 360, 449, 652
TspRI CASTG 3 cut(s) 19, 160, 325
XceI RCATGY 1 cut(s) 316
XspI CTAG 1 cut(s) 501
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.