pycom05g14760
NAC Family

Probably inactive leucine-rich repeat receptor-like protein kinase At5g48380

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0003453
Physical Location & Seq
Reverse (-)
18320798 .. 18321451
654 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g14760.1

Sequence Viewer

Length: 654 bp
ATGGCTTTCTTAATTCTTACGCTGTTCAGTCCTGCACAACATCTTGGAATTTTTGTTGGTGTTGTGTTCTGGTTATTGCTTAGTTGTAGCTTCTGCTTTGCTGTCACAAGCGACATCAATTGCTTAAAAAGTATCAAAGACTCGCTTAACGACCCTTTAGGCTACTTAAACTCTTCATGTGATTTCAGCAGCAAGACTGAAGGTTTCCCTTGTAACTTTATTAGAATCGAGTGTTGGCACCCTCACGAGAGCAAAGTTTTAAATATCAAGCTTTCGGATTTGGGACTCAAGGGCCAGTTTCCTCAGGGTGTAGCAAACCGCACAAGCTTAACAGGCCTAGATCTTTCAAGAAACAAGCTTAACGGACCTCGTCCTACTGATATTGGTAGATCCTTCCGTTCCTTACTTATCTTGATCTCTCATCCAACAGCTTCTCCGGGTCAATCCCAGCAAATCTTTCCAATTGTACCTATACGAATGTGCTTATGCTTGATAACAATAAGTTTACGGGTAAAATTCCTCCACAACTTAGCCAGCTCTCTAGGCTTACAAGATTTACCGTGGCCAACAATCTTCTGTCTGGTCAAGTCCCAGACTTTGGTGCTACTATTTTTATTACAACAAACAATAGTGGACTCTGCGGGGGCACGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8

Protein Analysis

218

Amino Acids

23.98

Weight (kDa)

8.94

Isoelectric Point (pI)

53.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 237
AccB7I CCANNNNNTGG 1 cut(s) 598
AciI CCGC 2 cut(s) 319, 641
AclWI GGATC 1 cut(s) 384
AcoI YGGCCR 1 cut(s) 563
AcsI RAATTY 2 cut(s) 48, 515
AcuI CTGAAG 1 cut(s) 219
AfaI GTAC 1 cut(s) 468
AfiI CCNNNNNNNGG 1 cut(s) 598
AgsI TTSAA 1 cut(s) 348
AjuI GAANNNNNNNTTGG 2 cut(s) 217, 249
AluBI AGCT 6 cut(s) 90, 271, 327, 358, 431, 537
AluI AGCT 6 cut(s) 90, 271, 327, 358, 431, 537
AlwI GGATC 1 cut(s) 384
AoxI GGCC 3 cut(s) 292, 334, 563
ApeKI GCWGC 1 cut(s) 189
ApoI RAATTY 2 cut(s) 48, 515
AspS9I GGNCC 2 cut(s) 292, 365
AsuC2I CCSGG 1 cut(s) 438
AvaII GGWCC 1 cut(s) 365
AxyI CCTNAGG 1 cut(s) 303
BaeGI GKGCMC 1 cut(s) 649
BalI TGGCCA 1 cut(s) 565
BanI GGYRCC 1 cut(s) 237
BauI CACGAG 1 cut(s) 245
BbvI GCAGC 1 cut(s) 201
BcnI CCSGG 1 cut(s) 438
BfaI CTAG 2 cut(s) 338, 542
BglII AGATCT 1 cut(s) 340
BisI GCNGC 1 cut(s) 190
BlsI GCNGC 1 cut(s) 191
Bme1390I CCNGG 1 cut(s) 438
Bme18I GGWCC 1 cut(s) 365
BmgT120I GGNCC 2 cut(s) 292, 365
BmiI GGNNCC 1 cut(s) 239
BmrFI CCNGG 1 cut(s) 438
BpuEI CTTGAG 1 cut(s) 272
BpuMI CCSGG 1 cut(s) 438
BsaJI CCNNGG 1 cut(s) 560
BsaXI ACNNNNNCTCC 2 cut(s) 418, 448
Bsc4I CCNNNNNNNGG 1 cut(s) 598
Bse1I ACTGG 1 cut(s) 295
Bse21I CCTNAGG 1 cut(s) 303
BseDI CCNNGG 1 cut(s) 560
BseGI GGATG 1 cut(s) 421
BseLI CCNNNNNNNGG 1 cut(s) 598
BseMII CTCAG 1 cut(s) 317
BseNI ACTGG 1 cut(s) 295
BseSI GKGCMC 1 cut(s) 649
BseXI GCAGC 1 cut(s) 201
BseYI CCCAGC 1 cut(s) 447
BsgI GTGCAG 1 cut(s) 18
BshFI GGCC 3 cut(s) 294, 336, 565
BshNI GGYRCC 1 cut(s) 237
BsiSI CCGG 1 cut(s) 437
BslFI GGGAC 2 cut(s) 297, 574
BslI CCNNNNNNNGG 1 cut(s) 598
BsmFI GGGAC 2 cut(s) 297, 574
BsnI GGCC 3 cut(s) 294, 336, 565
Bsp1286I GDGCHC 1 cut(s) 649
Bsp143I GATC 3 cut(s) 340, 389, 414
BspACI CCGC 2 cut(s) 319, 641
BspANI GGCC 3 cut(s) 294, 336, 565
BspCNI CTCAG 1 cut(s) 316
BspLI GGNNCC 1 cut(s) 239
BspPI GGATC 1 cut(s) 384
BspT107I GGYRCC 1 cut(s) 237
BsrI ACTGG 1 cut(s) 295
BssECI CCNNGG 1 cut(s) 560
BssMI GATC 3 cut(s) 340, 389, 414
BssSI CACGAG 1 cut(s) 245
Bst2BI CACGAG 1 cut(s) 245
Bst4CI ACNGT 1 cut(s) 561
Bst6I CTCTTC 1 cut(s) 178
BstC8I GCNNGC 1 cut(s) 535
BstDEI CTNAG 3 cut(s) 80, 303, 529
BstDSI CCRYGG 1 cut(s) 560
BstF5I GGATG 1 cut(s) 421
BstKTI GATC 3 cut(s) 343, 392, 417
BstMBI GATC 3 cut(s) 340, 389, 414
BstMWI GCNNNNNNNGC 2 cut(s) 333, 543
BstSCI CCNGG 1 cut(s) 436
BstSLI GKGCMC 1 cut(s) 649
BstV1I GCAGC 1 cut(s) 201
BstX2I RGATCY 2 cut(s) 340, 389
BstYI RGATCY 2 cut(s) 340, 389
Bsu36I CCTNAGG 1 cut(s) 303
BsuRI GGCC 3 cut(s) 294, 336, 565
BtgI CCRYGG 1 cut(s) 560
BtsCI GGATG 1 cut(s) 421
Cac8I GCNNGC 1 cut(s) 535
Cfr13I GGNCC 2 cut(s) 292, 365
Csp6I GTAC 1 cut(s) 467
CviAII CATG 1 cut(s) 177
CviQI GTAC 1 cut(s) 467
DdeI CTNAG 3 cut(s) 80, 303, 529
DpnI GATC 3 cut(s) 342, 391, 416
DpnII GATC 3 cut(s) 340, 389, 414
DraI TTTAAA 1 cut(s) 261
EaeI YGGCCR 1 cut(s) 563
Eam1104I CTCTTC 1 cut(s) 178
EarI CTCTTC 1 cut(s) 178
Eco147I AGGCCT 1 cut(s) 336
Eco47I GGWCC 1 cut(s) 365
Eco57I CTGAAG 1 cut(s) 219
Eco81I CCTNAGG 1 cut(s) 303
FaeI CATG 1 cut(s) 180
FaiI YATR 3 cut(s) 178, 473, 487
FalI AAGNNNNNCTT 2 cut(s) 129, 161
FaqI GGGAC 2 cut(s) 297, 574
FatI CATG 1 cut(s) 176
FauI CCCGC 1 cut(s) 634
Fnu4HI GCNGC 1 cut(s) 190
FokI GGATG 1 cut(s) 408
Fsp4HI GCNGC 1 cut(s) 190
FspBI CTAG 2 cut(s) 338, 542
GluI GCNGC 1 cut(s) 190
GsaI CCCAGC 1 cut(s) 451
HaeIII GGCC 3 cut(s) 294, 336, 565
HapII CCGG 1 cut(s) 437
Hin1II CATG 1 cut(s) 180
HindIII AAGCTT 3 cut(s) 269, 325, 356
HinfI GANTC 4 cut(s) 140, 225, 285, 635
HpaII CCGG 1 cut(s) 437
Hpy166II GTNNAC 2 cut(s) 506, 634
Hpy188I TCNGA 1 cut(s) 277
Hpy188III TCNNGA 3 cut(s) 245, 348, 412
Hpy8I GTNNAC 2 cut(s) 506, 634
HpyAV CCTTC 2 cut(s) 194, 403
HpyCH4III ACNGT 1 cut(s) 561
HpyCH4IV ACGT 1 cut(s) 649
HpyCH4V TGCA 1 cut(s) 35
HpyF10VI GCNNNNNNNGC 2 cut(s) 333, 543
HpyF3I CTNAG 3 cut(s) 80, 303, 529
HpySE526I ACGT 1 cut(s) 649
Hsp92II CATG 1 cut(s) 180
Kzo9I GATC 3 cut(s) 340, 389, 414
Lsp1109I GCAGC 1 cut(s) 201
MaeI CTAG 2 cut(s) 338, 542
MaeII ACGT 1 cut(s) 649
MaeIII GTNAC 2 cut(s) 103, 212
MalI GATC 3 cut(s) 342, 391, 416
MboI GATC 3 cut(s) 340, 389, 414
MboII GAAGA 2 cut(s) 165, 565
MfeI CAATTG 2 cut(s) 118, 462
MflI RGATCY 2 cut(s) 340, 389
MhlI GDGCHC 1 cut(s) 649
MlsI TGGCCA 1 cut(s) 565
MluCI AATT 5 cut(s) 12, 48, 118, 462, 515
MluNI TGGCCA 1 cut(s) 565
MlyI GAGTC 3 cut(s) 134, 279, 629
MmeI TCCRAC 1 cut(s) 449
MnlI CCTC 4 cut(s) 252, 312, 378, 530
Mox20I TGGCCA 1 cut(s) 565
MscI TGGCCA 1 cut(s) 565
MseI TTAA 7 cut(s) 11, 125, 147, 167, 260, 329, 360
Msp20I TGGCCA 1 cut(s) 565
MspI CCGG 1 cut(s) 437
MspR9I CCNGG 1 cut(s) 438
MunI CAATTG 2 cut(s) 118, 462
MwoI GCNNNNNNNGC 2 cut(s) 333, 543
NciI CCSGG 1 cut(s) 438
NdeII GATC 3 cut(s) 340, 389, 414
NlaIII CATG 1 cut(s) 180
NlaIV GGNNCC 1 cut(s) 239
NmuCI GTSAC 1 cut(s) 103
PceI AGGCCT 1 cut(s) 336
PfeI GAWTC 1 cut(s) 225
PflFI GACNNNGTC 1 cut(s) 369
PflMI CCANNNNNTGG 1 cut(s) 598
PkrI GCNGC 1 cut(s) 191
PleI GAGTC 3 cut(s) 134, 279, 629
PpsI GAGTC 3 cut(s) 134, 279, 629
PspFI CCCAGC 1 cut(s) 447
PspN4I GGNNCC 1 cut(s) 239
PspPI GGNCC 2 cut(s) 292, 365
PsuI RGATCY 2 cut(s) 340, 389
PsyI GACNNNGTC 1 cut(s) 369
RsaI GTAC 1 cut(s) 468
RsaNI GTAC 1 cut(s) 467
SaqAI TTAA 7 cut(s) 11, 125, 147, 167, 260, 329, 360
SatI GCNGC 1 cut(s) 190
Sau3AI GATC 3 cut(s) 340, 389, 414
Sau96I GGNCC 2 cut(s) 292, 365
SchI GAGTC 3 cut(s) 134, 279, 629
ScrFI CCNGG 1 cut(s) 438
SduI GDGCHC 1 cut(s) 649
SinI GGWCC 1 cut(s) 365
SmlI CTYRAG 1 cut(s) 287
SmoI CTYRAG 1 cut(s) 287
Sse9I AATT 5 cut(s) 12, 48, 118, 462, 515
SseBI AGGCCT 1 cut(s) 336
SsiI CCGC 2 cut(s) 319, 641
SspMI CTAG 2 cut(s) 338, 542
StuI AGGCCT 1 cut(s) 336
StyD4I CCNGG 1 cut(s) 436
TaaI ACNGT 1 cut(s) 561
TaiI ACGT 1 cut(s) 652
TaqI TCGA 1 cut(s) 228
TasI AATT 5 cut(s) 12, 48, 118, 462, 515
TfiI GAWTC 1 cut(s) 225
Tru1I TTAA 7 cut(s) 11, 125, 147, 167, 260, 329, 360
Tru9I TTAA 7 cut(s) 11, 125, 147, 167, 260, 329, 360
TseFI GTSAC 1 cut(s) 103
TseI GCWGC 1 cut(s) 189
Tsp45I GTSAC 1 cut(s) 103
TspDTI ATGAA 1 cut(s) 165
TspGWI ACGGA 2 cut(s) 378, 386
Tth111I GACNNNGTC 1 cut(s) 369
Van91I CCANNNNNTGG 1 cut(s) 598
VpaK11BI GGWCC 1 cut(s) 365
XapI RAATTY 2 cut(s) 48, 515
XspI CTAG 2 cut(s) 338, 542
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.