MD06G1160200.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0003877
Physical Location & Seq
Forward (+)
30156354 .. 30160017
3664 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1160200.v1.1.491

Sequence Viewer

Length: 2163 bp
ATGGCTCTCATCCGCCACCAACTCTCTCGTCCTCCGTCACTTCCCTCCTCCCGCAGCCACCCTCCACCAAACCACCACTTCCCAAAACCCTGCCTCTTCGTATTCTCCAAAAACCTCAGACTCTTCTCTCTCTACTCAGCTCCGCCCACTCCCTCCTCTCTCTCCTCCGCCCCCATTTTCCTCCCCTTTCTTCAAAACCAAGAGGAAGAAGACGAAGACGAAACTGAAGAAGAAACAGAAGAGCCACCAGCACTCGAAGAAGATGAAGAAGAGGAGGAGGACCCAGATGACCCAATTCTCAGATTCTTCAAGTCCCGCACTTCAACCCAAGACCCTGAGCGCGAAGGCAAGTTCTCTCTCCAAAAGAACCGCCGCTCCGCTTGGCGTTTGGCTGACGGTACCCACCTCGCAGACGAATCGGAACCCGAAACCGGCGTCAAAAAACTGCTCGGAGAACAGAAACAAGTTGGTCCAATCAAATTTGGTTCAAATGCATCGCCTGAGGGAATTGTCCAAGAGATTCTGCAAAAGGCAAGGACTTTGCCGCAGAATTTGACGTTGGGAGAGGCTTTGGGGGGTTTCGAAGGAAGGGTTGGGGAGAAAGAGTGTGTGAAAATATTGGAGGTGATGGGTGAGGAGGGGTTATTGGTGGGTTGCTTGTATTTCTTTGAGTGGATGGGTTTGCAGGAGCCGTCTCTTGTTACGCCTCGGGCGTGTTCGGTTTTGTTTCCGATGCTGGGGAGAGCTGGAATGGGGGATAAGTTGATGATTTTGTTTAGGAATTTACCGGCCAAGAAGGAGTTCTGGGATGTTCATGTGTATAATGCTGCAATTTCGGGCTTAATGTGCTCCAAGAGGTACGATGATGCTTGGAAGGTGTATGAGACTATGGAAGCAAATAATACCCTTCCGGATCATGTGACATGTTCAATCATGATTACTGTCATGAGAAAAGTTGGACGTAGTGCAAAAGATTCATGGCAGTTCTTCGAGAGAATGAACAGGAAAGGTGTCAGATGGAGTCAAGAAGTTTTGGGTGCTCTTATAAAATCATTCTGTGATGAGGGGTTGAAGAGAGAAGCCCTTATCATTCAAGTAGAAATGGAGAAGAAAGGGGTCTCATCAAACGCCATTGTGTATAACACTTTAATGGATGCCTTCTGTAATTCAAACCAAGTTGAAGAAGCTGAAGGTCTCTTTGCCGAGATGAAATCAAAAGGGATTAAACCAACAGCTGCCACCTTCAATGTTCTAATGAGTGCATACAGCAGAAAAATGGAGCCGGAGATTGTTGAGAAGCTGCTGGTCGAAATGCATGATATGGGACTAAAGCCAAATGTCAAATCCTATACTTGTCTTATCAGTGCATATGGGAGGCAGAAGAAGATGAGTGATATGGCTGCGGATGCTTTTTTGAGGATGAAGAAGGTTGGTATTCGTCCAACATCACATTCTTATACAGCTCTTATTCACGCGTTTTCAGTCAGCGGCTGGCATGAGAAGGCTTACATTGCATTCGAAAACATGCAAAAAGAAGGACTAAAGCCCTCCATAGAAACCTATACTGCTTTACTGGATGCATTTAGGCGTGCTGGTGACGCGCAGATGTTGATGAAAATTTGGAAACTGATGATCAAAGAGAAGATTGTGGGGACTAAGGTGACATACAACACTCTTCTCGATGGATTTGCCAAACAAGGGCACTATGTGGAAGCAAGAGATGTGATTTCCGAATTTGGTAATGTAGGCTTGCAGCCAACTGTGATGACATATAATATGCTGATGAATGCGTATGCACGGGGAGGGCAACACTCAAAGTTGCCACAGCTGTTGAAAGAGATGGCAGCTCTCAAGTTAAAACCTGATTCTGTAACTTACTCGACCATGATTTATGCCTATGTTCGTGTTCGTGATTTCAGACGAGCATTTTTCTACCACAAGCAAATGGTAAAAAATGGACAAGTGCCAGATGCCAGGTCTTATGAGAAGCTTAGGTCAATTTTGGATGTGAAAGCTGCACGGAAGAACAAAAAGGACAAGAGTGCTATACTCGGTATAATTAATAGCAAAATGGGTTTGTTGAAAGTTAAAAAGAAGGGAAAGAAAGATGAGTTCTGGAAGAACAAGAACAAGCGTTATGTCAGAACTGATAACTCAGAGTGA

Protein Analysis

721

Amino Acids

81.79

Weight (kDa)

8.93

Isoelectric Point (pI)

46.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 270 - 315 2.7e-10 PPR repeat family
PPR_long PF17177 282 - 419 1.8e-07 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 347 - 389 3.8e-07 PPR repeat family
PPR_3 PF13812 367 - 422 7e-12 Pentatricopeptide repeat domain
TPR_24 PF23276 368 - 462 8.7e-07 Fungal tetratrico peptide repeats
PPR_1 PF12854 371 - 404 2e-08 PPR repeat
PPR_2 PF13041 376 - 423 3e-16 PPR repeat family
PPR PF01535 379 - 408 1.1e-08 PPR repeat
PPR_long PF17177 399 - 503 1.8e-08 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 410 - 457 9.1e-10 PPR repeat family
PPR_3 PF13812 433 - 493 4.9e-11 Pentatricopeptide repeat domain
PPR_2 PF13041 445 - 493 2.3e-10 PPR repeat family
TPR_24 PF23276 462 - 529 6e-06 Fungal tetratrico peptide repeats
PPR_long PF17177 465 - 542 1e-06 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 485 - 528 1.2e-08 PPR repeat family
PPR_3 PF13812 506 - 561 9.3e-12 Pentatricopeptide repeat domain
PPR_2 PF13041 516 - 565 3e-08 PPR repeat family
PPR_3 PF13812 554 - 598 7.1e-08 Pentatricopeptide repeat domain
PPR_2 PF13041 554 - 600 1.3e-10 PPR repeat family
PPR_2 PF13041 586 - 634 2.4e-11 PPR repeat family
PPR_2 PF13041 621 - 661 7.2e-07 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1046
Acc65I GGTACC 1 cut(s) 398
AccB1I GGYRCC 1 cut(s) 398
AccBSI CCGCTC 1 cut(s) 375
AccII CGCG 3 cut(s) 342, 1475, 1601
AccIII TCCGGA 1 cut(s) 910
AclWI GGATC 1 cut(s) 921
AcoI YGGCCR 1 cut(s) 789
AcsI RAATTY 5 cut(s) 479, 550, 781, 1617, 1733
AcuI CTGAAG 2 cut(s) 246, 1209
AcyI GRCGYC 1 cut(s) 435
AdeI CACNNNGTG 1 cut(s) 1708
AfaI GTAC 2 cut(s) 400, 860
AfiI CCNNNNNNNGG 3 cut(s) 431, 737, 1698
AflIII ACRYGT 2 cut(s) 923, 1473
AjnI CCWGG 1 cut(s) 1973
AjuI GAANNNNNNNTTGG 8 cut(s) 542, 574, 576, 608, 785, 817, 1435, 1467
AloI GAACNNNNNNTCC 2 cut(s) 359, 391
Alw21I GWGCWC 2 cut(s) 851, 1042
Alw26I GTCTC 4 cut(s) 699, 878, 1123, 1199
AlwI GGATC 1 cut(s) 921
AlwNI CAGNNNCTG 1 cut(s) 1491
Ama87I CYCGRG 1 cut(s) 708
Aor13HI TCCGGA 1 cut(s) 910
AoxI GGCC 1 cut(s) 789
ApeKI GCWGC 8 cut(s) 54, 827, 1235, 1300, 1400, 1753, 1844, 2015
ApoI RAATTY 5 cut(s) 479, 550, 781, 1617, 1733
ArsI GACNNNNNNTTYG 2 cut(s) 420, 452
AseI ATTAAT 1 cut(s) 2061
Asp700I GAANNNNTTC 1 cut(s) 800
Asp718I GGTACC 1 cut(s) 398
AspLEI GCGC 2 cut(s) 342, 1603
AspS9I GGNCC 2 cut(s) 280, 470
AsuHPI GGTGA 4 cut(s) 637, 644, 1607, 1672
AsuII TTCGAA 2 cut(s) 582, 1518
AvaI CYCGRG 1 cut(s) 708
AvaII GGWCC 2 cut(s) 280, 470
AxyI CCTNAGG 1 cut(s) 501
BaeGI GKGCMC 1 cut(s) 1704
BanI GGYRCC 1 cut(s) 398
BbsI GAAGAC 2 cut(s) 216, 222
Bbv12I GWGCWC 2 cut(s) 851, 1042
BbvI GCAGC 8 cut(s) 66, 814, 1222, 1287, 1387, 1765, 1856, 2002
BccI CCATC 5 cut(s) 622, 670, 1011, 1676, 1834
BceAI ACGGC 1 cut(s) 676
BcgI CGANNNNNNTGC 4 cut(s) 399, 433, 1670, 1704
BciT130I CCWGG 1 cut(s) 1975
BclI TGATCA 1 cut(s) 1632
BcoDI GTCTC 4 cut(s) 699, 878, 1123, 1199
Bme1390I CCNGG 1 cut(s) 1975
Bme18I GGWCC 2 cut(s) 280, 470
BmeT110I CYCGRG 1 cut(s) 708
BmgT120I GGNCC 2 cut(s) 280, 470
BmiI GGNNCC 5 cut(s) 282, 400, 423, 690, 1281
BmrFI CCNGG 1 cut(s) 1975
BmsI GCATC 7 cut(s) 503, 723, 856, 1144, 1396, 1567, 1960
BpiI GAAGAC 2 cut(s) 216, 222
Bpu10I CCTNAGC 2 cut(s) 336, 1991
Bpu14I TTCGAA 2 cut(s) 582, 1518
BpuEI CTTGAG 1 cut(s) 1835
BsaHI GRCGYC 1 cut(s) 435
BsaI GGTCTC 2 cut(s) 1123, 1199
BsaJI CCNNGG 1 cut(s) 707
BsaWI WCCGGW 1 cut(s) 910
BsaXI ACNNNNNCTCC 4 cut(s) 359, 389, 590, 620
Bsc4I CCNNNNNNNGG 3 cut(s) 431, 737, 1698
Bse118I RCCGGY 2 cut(s) 431, 787
Bse1I ACTGG 1 cut(s) 1578
Bse21I CCTNAGG 1 cut(s) 501
Bse3DI GCAATG 1 cut(s) 1509
BseAI TCCGGA 1 cut(s) 910
BseBI CCWGG 1 cut(s) 1975
BseDI CCNNGG 1 cut(s) 707
BseGI GGATG 8 cut(s) 9, 681, 814, 1159, 1411, 1425, 1582, 2011
BseLI CCNNNNNNNGG 3 cut(s) 431, 737, 1698
BseMI GCAATG 1 cut(s) 1509
BseMII CTCAG 5 cut(s) 130, 150, 313, 327, 492
BseNI ACTGG 1 cut(s) 1578
BseRI GAGGAG 6 cut(s) 37, 145, 154, 287, 290, 650
BseSI GKGCMC 1 cut(s) 1704
BseXI GCAGC 8 cut(s) 66, 814, 1222, 1287, 1387, 1765, 1856, 2002
BseYI CCCAGC 1 cut(s) 736
BsgI GTGCAG 1 cut(s) 2001
Bsh1236I CGCG 3 cut(s) 342, 1475, 1601
BshFI GGCC 1 cut(s) 791
BshNI GGYRCC 1 cut(s) 398
BsiHKAI GWGCWC 2 cut(s) 851, 1042
BsiHKCI CYCGRG 1 cut(s) 708
BsiSI CCGG 4 cut(s) 432, 788, 911, 1283
BslFI GGGAC 3 cut(s) 298, 1338, 1666
BslI CCNNNNNNNGG 3 cut(s) 431, 737, 1698
BsmAI GTCTC 4 cut(s) 699, 878, 1123, 1199
BsmBI CGTCTC 1 cut(s) 699
BsmFI GGGAC 3 cut(s) 298, 1338, 1666
BsmI GAATGC 2 cut(s) 1514, 1792
BsnI GGCC 1 cut(s) 791
Bso31I GGTCTC 2 cut(s) 1123, 1199
BsoBI CYCGRG 1 cut(s) 708
Bsp119I TTCGAA 2 cut(s) 582, 1518
Bsp1286I GDGCHC 3 cut(s) 851, 1042, 1704
Bsp13I TCCGGA 1 cut(s) 910
Bsp143I GATC 2 cut(s) 913, 1632
BspANI GGCC 1 cut(s) 791
BspCNI CTCAG 5 cut(s) 129, 149, 312, 328, 493
BspEI TCCGGA 1 cut(s) 910
BspFNI CGCG 3 cut(s) 342, 1475, 1601
BspHI TCATGA 2 cut(s) 933, 945
BspLI GGNNCC 5 cut(s) 282, 400, 423, 690, 1281
BspPI GGATC 1 cut(s) 921
BspQI GCTCTTC 1 cut(s) 234
BspT104I TTCGAA 2 cut(s) 582, 1518
BspT107I GGYRCC 1 cut(s) 398
BspTNI GGTCTC 2 cut(s) 1123, 1199
BsrBI CCGCTC 1 cut(s) 375
BsrDI GCAATG 1 cut(s) 1509
BsrFI RCCGGY 2 cut(s) 431, 787
BsrI ACTGG 1 cut(s) 1578
BssAI RCCGGY 2 cut(s) 431, 787
BssECI CCNNGG 1 cut(s) 707
BssMI GATC 2 cut(s) 913, 1632
BssNI GRCGYC 1 cut(s) 435
Bst2UI CCWGG 1 cut(s) 1975
Bst4CI ACNGT 3 cut(s) 398, 943, 1762
Bst6I CTCTTC 6 cut(s) 101, 128, 234, 264, 1067, 1680
BstACI GRCGYC 1 cut(s) 435
BstBI TTCGAA 2 cut(s) 582, 1518
BstC8I GCNNGC 3 cut(s) 1493, 1590, 1751
BstDEI CTNAG 8 cut(s) 116, 136, 299, 336, 501, 1656, 1991, 2155
BstF5I GGATG 8 cut(s) 9, 681, 814, 1159, 1411, 1425, 1582, 2011
BstFNI CGCG 3 cut(s) 342, 1475, 1601
BstHHI GCGC 2 cut(s) 342, 1603
BstKTI GATC 2 cut(s) 916, 1635
BstMAI GTCTC 4 cut(s) 699, 878, 1123, 1199
BstMBI GATC 2 cut(s) 913, 1632
BstMWI GCNNNNNNNGC 4 cut(s) 846, 1406, 1511, 1598
BstNI CCWGG 1 cut(s) 1975
BstNSI RCATGY 2 cut(s) 927, 1528
BstSCI CCNGG 1 cut(s) 1973
BstSLI GKGCMC 1 cut(s) 1704
BstUI CGCG 3 cut(s) 342, 1475, 1601
BstV1I GCAGC 8 cut(s) 66, 814, 1222, 1287, 1387, 1765, 1856, 2002
BstV2I GAAGAC 2 cut(s) 216, 222
Bsu36I CCTNAGG 1 cut(s) 501
BsuRI GGCC 1 cut(s) 791
BtgZI GCGATG 1 cut(s) 480
BtsCI GGATG 8 cut(s) 9, 681, 814, 1159, 1411, 1425, 1582, 2011
BtsIMutI CAGTG 1 cut(s) 1369
Cac8I GCNNGC 3 cut(s) 1493, 1590, 1751
CaiI CAGNNNCTG 1 cut(s) 1491
CciI TCATGA 2 cut(s) 933, 945
CfoI GCGC 2 cut(s) 342, 1603
Cfr10I RCCGGY 2 cut(s) 431, 787
Cfr13I GGNCC 2 cut(s) 280, 470
CseI GACGC 2 cut(s) 424, 1607
Csp6I GTAC 2 cut(s) 399, 859
CspCI CAANNNNNGTGG 2 cut(s) 1812, 1847
CviQI GTAC 2 cut(s) 399, 859
DdeI CTNAG 8 cut(s) 116, 136, 299, 336, 501, 1656, 1991, 2155
DpnI GATC 2 cut(s) 915, 1634
DpnII GATC 2 cut(s) 913, 1632
DraIII CACNNNGTG 1 cut(s) 1708
EaeI YGGCCR 1 cut(s) 789
Eam1104I CTCTTC 6 cut(s) 101, 128, 234, 264, 1067, 1680
EarI CTCTTC 6 cut(s) 101, 128, 234, 264, 1067, 1680
EciI GGCGGA 2 cut(s) 132, 157
Eco31I GGTCTC 2 cut(s) 1123, 1199
Eco47I GGWCC 2 cut(s) 280, 470
Eco57I CTGAAG 2 cut(s) 246, 1209
Eco81I CCTNAGG 1 cut(s) 501
Eco88I CYCGRG 1 cut(s) 708
EcoO109I RGGNCCY 1 cut(s) 280
EcoRII CCWGG 1 cut(s) 1973
EcoT22I ATGCAT 3 cut(s) 496, 1317, 1582
Esp3I CGTCTC 1 cut(s) 699
FaqI GGGAC 3 cut(s) 298, 1338, 1666
FauI CCCGC 2 cut(s) 59, 323
FauNDI CATATG 1 cut(s) 1369
FbaI TGATCA 1 cut(s) 1632
FokI GGATG 7 cut(s) 688, 821, 1166, 1418, 1432, 1589, 2018
GlaI GCGC 2 cut(s) 341, 1602
GsaI CCCAGC 1 cut(s) 740
HaeIII GGCC 1 cut(s) 791
HapII CCGG 4 cut(s) 432, 788, 911, 1283
HgaI GACGC 2 cut(s) 424, 1607
HhaI GCGC 2 cut(s) 342, 1603
Hin1I GRCGYC 1 cut(s) 435
Hin6I GCGC 2 cut(s) 340, 1601
HinP1I GCGC 2 cut(s) 340, 1601
HindIII AAGCTT 1 cut(s) 1988
HinfI GANTC 7 cut(s) 120, 303, 416, 520, 974, 1021, 1865
HpaII CCGG 4 cut(s) 432, 788, 911, 1283
HphI GGTGA 4 cut(s) 637, 644, 1607, 1672
Hpy188III TCNNGA 8 cut(s) 911, 934, 946, 991, 1025, 1679, 1910, 2116
HpyCH4III ACNGT 3 cut(s) 398, 943, 1762
HpyCH4IV ACGT 2 cut(s) 557, 961
HpyF10VI GCNNNNNNNGC 4 cut(s) 846, 1406, 1511, 1598
HpyF3I CTNAG 8 cut(s) 116, 136, 299, 336, 501, 1656, 1991, 2155
HpySE526I ACGT 2 cut(s) 557, 961
Hsp92I GRCGYC 1 cut(s) 435
HspAI GCGC 2 cut(s) 340, 1601
Kpn2I TCCGGA 1 cut(s) 910
KpnI GGTACC 1 cut(s) 402
Ksp22I TGATCA 1 cut(s) 1632
Kzo9I GATC 2 cut(s) 913, 1632
LguI GCTCTTC 1 cut(s) 234
LmnI GCTCC 5 cut(s) 145, 380, 688, 854, 1279
Lsp1109I GCAGC 8 cut(s) 66, 814, 1222, 1287, 1387, 1765, 1856, 2002
LweI GCATC 7 cut(s) 503, 723, 856, 1144, 1396, 1567, 1960
MaeII ACGT 2 cut(s) 557, 961
MaeIII GTNAC 6 cut(s) 36, 700, 919, 1595, 1660, 1870
MalI GATC 2 cut(s) 915, 1634
MbiI CCGCTC 1 cut(s) 375
MboI GATC 2 cut(s) 913, 1632
MhlI GDGCHC 3 cut(s) 851, 1042, 1704
MluI ACGCGT 1 cut(s) 1473
MlyI GAGTC 2 cut(s) 114, 1030
MmeI TCCRAC 2 cut(s) 937, 1466
Mph1103I ATGCAT 3 cut(s) 496, 1317, 1582
MroI TCCGGA 1 cut(s) 910
MroXI GAANNNNTTC 1 cut(s) 800
MseI TTAA 6 cut(s) 842, 1148, 1224, 1856, 2061, 2088
MslI CAYNNNNRTG 1 cut(s) 1148
MspA1I CMGCKG 3 cut(s) 1235, 1488, 1828
MspI CCGG 4 cut(s) 432, 788, 911, 1283
MspR9I CCNGG 1 cut(s) 1975
Mva1269I GAATGC 2 cut(s) 1514, 1792
MvaI CCWGG 1 cut(s) 1975
MvnI CGCG 3 cut(s) 342, 1475, 1601
MwoI GCNNNNNNNGC 4 cut(s) 846, 1406, 1511, 1598
NdeI CATATG 1 cut(s) 1369
NdeII GATC 2 cut(s) 913, 1632
NlaIV GGNNCC 5 cut(s) 282, 400, 423, 690, 1281
NmeAIII GCCGAG 1 cut(s) 1228
NmuCI GTSAC 4 cut(s) 36, 919, 1595, 1660
NsiI ATGCAT 3 cut(s) 496, 1317, 1582
NspI RCATGY 2 cut(s) 927, 1528
NspV TTCGAA 2 cut(s) 582, 1518
PagI TCATGA 2 cut(s) 933, 945
PciI ACATGT 1 cut(s) 923
PciSI GCTCTTC 1 cut(s) 234
PcsI WCGNNNNNNNCGW 1 cut(s) 710
PctI GAATGC 2 cut(s) 1514, 1792
PdmI GAANNNNTTC 1 cut(s) 800
PfeI GAWTC 5 cut(s) 303, 416, 520, 974, 1865
PleI GAGTC 2 cut(s) 114, 1029
PpsI GAGTC 2 cut(s) 114, 1029
PpuMI RGGWCCY 1 cut(s) 280
PscI ACATGT 1 cut(s) 923
PshBI ATTAAT 1 cut(s) 2061
PsiI TTATAA 1 cut(s) 1046
Psp5II RGGWCCY 1 cut(s) 280
Psp6I CCWGG 1 cut(s) 1973
PspFI CCCAGC 1 cut(s) 736
PspGI CCWGG 1 cut(s) 1973
PspN4I GGNNCC 5 cut(s) 282, 400, 423, 690, 1281
PspPI GGNCC 2 cut(s) 280, 470
PspPPI RGGWCCY 1 cut(s) 280
PstNI CAGNNNCTG 1 cut(s) 1491
PvuII CAGCTG 2 cut(s) 1235, 1828
RsaI GTAC 2 cut(s) 400, 860
RsaNI GTAC 2 cut(s) 399, 859
RseI CAYNNNNRTG 1 cut(s) 1148
SapI GCTCTTC 1 cut(s) 234
SaqAI TTAA 6 cut(s) 842, 1148, 1224, 1856, 2061, 2088
Sau3AI GATC 2 cut(s) 913, 1632
Sau96I GGNCC 2 cut(s) 280, 470
SchI GAGTC 2 cut(s) 114, 1030
ScrFI CCNGG 1 cut(s) 1975
SduI GDGCHC 3 cut(s) 851, 1042, 1704
SfaNI GCATC 7 cut(s) 503, 723, 856, 1144, 1396, 1567, 1960
SfuI TTCGAA 2 cut(s) 582, 1518
SinI GGWCC 2 cut(s) 280, 470
SmiMI CAYNNNNRTG 1 cut(s) 1148
SmlI CTYRAG 1 cut(s) 1850
SmoI CTYRAG 1 cut(s) 1850
SspI AATATT 1 cut(s) 618
StyD4I CCNGG 1 cut(s) 1973
TaaI ACNGT 3 cut(s) 398, 943, 1762
TaiI ACGT 2 cut(s) 560, 964
TaqI TCGA 7 cut(s) 255, 582, 990, 1308, 1518, 1680, 1880
TauI GCSGC 3 cut(s) 375, 547, 1491
TfiI GAWTC 5 cut(s) 303, 416, 520, 974, 1865
Tru1I TTAA 6 cut(s) 842, 1148, 1224, 1856, 2061, 2088
Tru9I TTAA 6 cut(s) 842, 1148, 1224, 1856, 2061, 2088
TscAI CASTG 1 cut(s) 1369
TseFI GTSAC 4 cut(s) 36, 919, 1595, 1660
TseI GCWGC 8 cut(s) 54, 827, 1235, 1300, 1400, 1753, 1844, 2015
Tsp45I GTSAC 4 cut(s) 36, 919, 1595, 1660
TspDTI ATGAA 8 cut(s) 279, 803, 966, 1013, 1223, 1436, 1628, 1799
TspGWI ACGGA 2 cut(s) 24, 2035
TspRI CASTG 1 cut(s) 1369
VpaK11BI GGWCC 2 cut(s) 280, 470
VspI ATTAAT 1 cut(s) 2061
XapI RAATTY 5 cut(s) 479, 550, 781, 1617, 1733
XceI RCATGY 2 cut(s) 927, 1528
XmnI GAANNNNTTC 1 cut(s) 800
Zsp2I ATGCAT 3 cut(s) 496, 1317, 1582
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.