Rorug02G0116400

(R)-mandelonitrile lyase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000428
Physical Location & Seq
Forward (+)
9888599 .. 9891330
2732 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0116400.1

Sequence Viewer

Length: 1005 bp
ATGAATTACAACGATACCCTGTTATCTCCTCTCCCAATAAATTTTCAATCTGACGATTCGTCACCAGTATGGCTCAACAAGGCAGACAATGCATGGCAACTCACAGCAACCACCATGGTAGCCATGCAAAGTGTTCCGGGGCTCGTGATACTCTATGGTTCCATGGTCAAGAAGAAATGGGCGGTGAACTCCGCGTTCATGGCCCTATACGCCTTTGCCGCGGTGCTCTTGTGTTGGGTGCTTTGGGCTCATAACATGTCATTTGGTATTAGGCTCACCCCATTTGTGGGAAGGCCTGGTTTTGCCATGACCCCAAATATCCTGCACCAATTTGCAGGCTATTATGTCCCAAAGGCTGACTACGTGTACTTTCAATTTGTTTTTGCTGCTATTACTGTGATATTGCTAGCTGGGTCTTTGCTTGGGAGGATGAACTTCTATGCTTGGATGTTGTTTGTACCAATGTGGCTCACACTGTCTTACTCAGTTGGGGCTTTTAGTATTTGGGCAAGTGCTGGATTTCTTGAGAAATACATCATTGATTATGCTGGCGGATTTGTGATTCATCTTTCTTCTGGTATAGCTGGTTTTATTGCTGCTTATTGGGTAGGACCAAGGCAATCACATGATAGGCAACACTTTCCACCAAACAACATAATTCACATGTTGGGAGGTGCAGGGTTTCTATGGCTTGGTTGGACAGGCTTCAATGGTGGCTCTCCATTTGCAGCAAATGAGATTGCGTCGTTGGCCATTCTCAACACCCATGTCTGCACTGCTACCAGCCTTTTGGTTTGGGTTTCCCTTGATATGATTGTGTACAAGAAAAGCTCTGTGATTGGCGCGGTACAGGGAATGATCACTGGCCTTGTCTGCATCACCCCCGGTGCAGCATTCCAACCAAATAATATATACATGAGCAGGGATTGTGGAGCCATGGGCAGCCATACTAATGGGAGCATCGTCTGGTTCGATACCTTGGTACACCATGATGGTATTGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00460 ko01110 map00460 map01110
Pfam Domains
Protein Families
GMC_oxred_C GMC_oxred_N

Protein Analysis

334

Amino Acids

36.62

Weight (kDa)

7.74

Isoelectric Point (pI)

37.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ammonium_transp PF00909 31 - 299 4.8e-50 Ammonium Transporter Family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000428)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g09331 FvH4_1g09661 FvH4_1g21942 FvH4_2g05841 FvH4_2g06090 FvH4_3g02580 FvH4_3g02591 FvH4_6g51091 FvH4_7g18893 FvH4_7g20022 FvH4_7g20111
malus_domestica MD09G1117600.v1.1 MD17G1117300.v1.1 MD17G1286200.v1.1
prunus_persica Prupe.8G124500_v2.0.a1 Prupe.8G124700_v2.0.a1 Prupe.8G124700_v2.0.a1
pyrus_communis pycom17g10120
rosa_chinensis RchiOBHm_Chr1g0354511 RchiOBHm_Chr1g0360131 RchiOBHm_Chr1g0365181 RchiOBHm_Chr1g0365201 RchiOBHm_Chr2g0096151 RchiOBHm_Chr2g0113181 RchiOBHm_Chr6g0257131 RchiOBHm_Chr6g0257771 RchiOBHm_Chr6g0265441 RchiOBHm_Chr6g0265451 RchiOBHm_Chr6g0265471 RchiOBHm_Chr6g0265481
rosa_multiflora Rmu_co8204816.1_g000001 Rmu_co8281643.1_g000001 Rmu_sc0000146.1_g000019 Rmu_sc0000217.1_g000012 Rmu_sc0001530.1_g000020 Rmu_sc0003520.1_g000002 Rmu_sc0003983.1_g000008 Rmu_sc0004419.1_g000008 Rmu_sc0004448.1_g000035 Rmu_sc0004896.1_g000001 Rmu_sc0005996.1_g000016 Rmu_sc0006683.1_g000002 Rmu_sc0007813.1_g000006 Rmu_sc0008035.1_g000004 Rmu_sc0008490.1_g000002 Rmu_sc0008742.1_g000001 Rmu_sc0010908.1_g000002 Rmu_sc0010908.1_g000007 Rmu_sc0011315.1_g000015 Rmu_sc0013544.1_g000002 Rmu_sc0014962.1_g000001 Rmu_sc0015275.1_g000003 Rmu_sc0017847.1_g000006 Rmu_sc0024070.1_g000003 Rmu_sc0040696.1_g000001 Rmu_ssc0000066.1_g000007 Rmu_ssc0000368.1_g000042
rosa_roxburghii Rroxscaffold_175G00432410 Rroxscaffold_2G00129540 Rroxscaffold_2G00146070 Rroxscaffold_6G00430280 Rroxscaffold_7G00202650 Rroxscaffold_7G00208930
rosa_rugosa Rorug01G0302700 Rorug01G0302800 Rorug02G0116400 Rorug04G0099000 Rorug05G0568500 Rorug05G0575100
rosa_samantha Rh1CG237300 Rh1CG272100 Rh1CG307500 Rh2BG009200 Rh2DG103600 Rh2DG169700 Rh2DG169800 Rh2DG248200 Rh2DG609700 Rh3AG050300 Rh5AG024900 Rh5AG025400 Rh6AG092900 Rh6BG079600 Rh6BG083600 Rh6BG137900 Rh6BG138600 Rh6BG138900 Rh6BG139000 Rh6CG136100
rosa_wichuraiana Rw1G022220 Rw2G008030 Rw2G012930 Rw5G002340 Rw6G008000 Rw6G011990 Rw6G012000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 194, 221, 845
AciI CCGC 6 cut(s) 182, 192, 219, 221, 552, 845
AcoI YGGCCR 1 cut(s) 750
AcsI RAATTY 1 cut(s) 40
AfaI GTAC 5 cut(s) 368, 459, 821, 849, 984
AfiI CCNNNNNNNGG 2 cut(s) 286, 287
AflIII ACRYGT 3 cut(s) 255, 363, 663
AgsI TTSAA 3 cut(s) 47, 374, 709
AhdI GACNNNNNGTC 1 cut(s) 58
AjnI CCWGG 1 cut(s) 295
AluBI AGCT 3 cut(s) 410, 584, 831
AluI AGCT 3 cut(s) 410, 584, 831
Alw21I GWGCWC 1 cut(s) 228
AoxI GGCC 4 cut(s) 201, 293, 750, 865
ApeKI GCWGC 5 cut(s) 386, 596, 728, 890, 942
ApoI RAATTY 1 cut(s) 40
AspLEI GCGC 1 cut(s) 845
AspS9I GGNCC 2 cut(s) 202, 611
AsuC2I CCSGG 2 cut(s) 138, 885
AsuHPI GGTGA 4 cut(s) 54, 196, 268, 871
AsuNHI GCTAGC 1 cut(s) 406
AvaII GGWCC 1 cut(s) 611
BaeI ACNNNNGTAYC 2 cut(s) 966, 999
BalI TGGCCA 1 cut(s) 752
BanII GRGCYC 2 cut(s) 144, 250
BauI CACGAG 1 cut(s) 143
Bbv12I GWGCWC 1 cut(s) 228
BbvI GCAGC 5 cut(s) 373, 583, 740, 902, 954
BccI CCATC 1 cut(s) 986
BciT130I CCWGG 1 cut(s) 297
BclI TGATCA 1 cut(s) 858
BcnI CCSGG 2 cut(s) 138, 885
BfaI CTAG 1 cut(s) 407
BisI GCNGC 6 cut(s) 219, 387, 597, 729, 891, 943
BlsI GCNGC 6 cut(s) 220, 388, 598, 730, 892, 944
Bme1390I CCNGG 3 cut(s) 138, 297, 885
Bme18I GGWCC 1 cut(s) 611
BmeRI GACNNNNNGTC 1 cut(s) 58
BmgT120I GGNCC 2 cut(s) 202, 611
BmiI GGNNCC 2 cut(s) 160, 934
BmrFI CCNGG 3 cut(s) 138, 297, 885
BmsI GCATC 2 cut(s) 885, 969
BmtI GCTAGC 1 cut(s) 410
BpuEI CTTGAG 1 cut(s) 545
BpuMI CCSGG 2 cut(s) 138, 885
BsaAI YACGTR 1 cut(s) 364
BsaJI CCNNGG 8 cut(s) 114, 137, 162, 219, 614, 883, 936, 978
Bsc4I CCNNNNNNNGG 2 cut(s) 286, 287
Bse1I ACTGG 2 cut(s) 65, 868
BseBI CCWGG 1 cut(s) 297
BseDI CCNNGG 8 cut(s) 114, 137, 162, 219, 614, 883, 936, 978
BseGI GGATG 2 cut(s) 435, 453
BseLI CCNNNNNNNGG 2 cut(s) 286, 287
BseMII CTCAG 1 cut(s) 498
BseNI ACTGG 2 cut(s) 65, 868
BseRI GAGGAG 1 cut(s) 18
BseXI GCAGC 5 cut(s) 373, 583, 740, 902, 954
BseYI CCCAGC 1 cut(s) 410
BsgI GTGCAG 4 cut(s) 308, 696, 757, 909
Bsh1236I CGCG 3 cut(s) 194, 221, 845
BshFI GGCC 4 cut(s) 203, 295, 752, 867
BsiHKAI GWGCWC 1 cut(s) 228
BsiSI CCGG 2 cut(s) 137, 885
BslFI GGGAC 1 cut(s) 332
BslI CCNNNNNNNGG 2 cut(s) 286, 287
BsmFI GGGAC 1 cut(s) 332
BsmI GAATGC 1 cut(s) 893
BsnI GGCC 4 cut(s) 203, 295, 752, 867
Bsp1286I GDGCHC 3 cut(s) 144, 228, 250
Bsp1407I TGTACA 1 cut(s) 819
Bsp143I GATC 1 cut(s) 858
Bsp19I CCATGG 3 cut(s) 114, 162, 936
BspACI CCGC 6 cut(s) 182, 192, 219, 221, 552, 845
BspANI GGCC 4 cut(s) 203, 295, 752, 867
BspCNI CTCAG 1 cut(s) 497
BspFNI CGCG 3 cut(s) 194, 221, 845
BspLI GGNNCC 2 cut(s) 160, 934
BspOI GCTAGC 1 cut(s) 410
BsrGI TGTACA 1 cut(s) 819
BsrI ACTGG 2 cut(s) 65, 868
BssECI CCNNGG 8 cut(s) 114, 137, 162, 219, 614, 883, 936, 978
BssMI GATC 1 cut(s) 858
BssSI CACGAG 1 cut(s) 143
BssT1I CCWWGG 5 cut(s) 114, 162, 614, 936, 978
Bst2BI CACGAG 1 cut(s) 143
Bst2UI CCWGG 1 cut(s) 297
Bst4CI ACNGT 2 cut(s) 397, 477
BstAPI GCANNNNNTGC 1 cut(s) 89
BstAUI TGTACA 1 cut(s) 819
BstBAI YACGTR 1 cut(s) 364
BstC8I GCNNGC 3 cut(s) 337, 408, 550
BstDEI CTNAG 1 cut(s) 484
BstDSI CCRYGG 4 cut(s) 114, 162, 219, 936
BstF5I GGATG 2 cut(s) 435, 453
BstFNI CGCG 3 cut(s) 194, 221, 845
BstHHI GCGC 1 cut(s) 845
BstKTI GATC 1 cut(s) 861
BstMBI GATC 1 cut(s) 858
BstMWI GCNNNNNNNGC 6 cut(s) 89, 200, 209, 218, 749, 873
BstNI CCWGG 1 cut(s) 297
BstNSI RCATGY 2 cut(s) 259, 667
BstSCI CCNGG 3 cut(s) 136, 295, 883
BstUI CGCG 3 cut(s) 194, 221, 845
BstV1I GCAGC 5 cut(s) 373, 583, 740, 902, 954
BstXI CCANNNNNNTGG 2 cut(s) 790, 953
BsuRI GGCC 4 cut(s) 203, 295, 752, 867
BtgI CCRYGG 4 cut(s) 114, 162, 219, 936
BtsCI GGATG 2 cut(s) 435, 453
BtsI GCAGTG 1 cut(s) 774
BtsIMutI CAGTG 3 cut(s) 473, 774, 861
Cac8I GCNNGC 3 cut(s) 337, 408, 550
CfoI GCGC 1 cut(s) 845
Cfr13I GGNCC 2 cut(s) 202, 611
Cfr42I CCGCGG 1 cut(s) 222
CseI GACGC 1 cut(s) 732
Csp6I GTAC 5 cut(s) 367, 458, 820, 848, 983
CviQI GTAC 5 cut(s) 367, 458, 820, 848, 983
DdeI CTNAG 1 cut(s) 484
DpnI GATC 1 cut(s) 860
DpnII GATC 1 cut(s) 858
DriI GACNNNNNGTC 1 cut(s) 58
EaeI YGGCCR 1 cut(s) 750
Eam1105I GACNNNNNGTC 1 cut(s) 58
EciI GGCGGA 1 cut(s) 567
Eco130I CCWWGG 5 cut(s) 114, 162, 614, 936, 978
Eco147I AGGCCT 1 cut(s) 295
Eco24I GRGCYC 2 cut(s) 144, 250
Eco47I GGWCC 1 cut(s) 611
EcoRII CCWGG 1 cut(s) 295
EcoT14I CCWWGG 5 cut(s) 114, 162, 614, 936, 978
EcoT22I ATGCAT 1 cut(s) 94
EcoT38I GRGCYC 2 cut(s) 144, 250
ErhI CCWWGG 5 cut(s) 114, 162, 614, 936, 978
FaqI GGGAC 1 cut(s) 332
FbaI TGATCA 1 cut(s) 858
Fnu4HI GCNGC 6 cut(s) 219, 387, 597, 729, 891, 943
FokI GGATG 2 cut(s) 442, 460
FriOI GRGCYC 2 cut(s) 144, 250
Fsp4HI GCNGC 6 cut(s) 219, 387, 597, 729, 891, 943
FspBI CTAG 1 cut(s) 407
GlaI GCGC 1 cut(s) 844
GluI GCNGC 6 cut(s) 219, 387, 597, 729, 891, 943
GsaI CCCAGC 1 cut(s) 414
HaeIII GGCC 4 cut(s) 203, 295, 752, 867
HapII CCGG 2 cut(s) 137, 885
HgaI GACGC 1 cut(s) 732
HhaI GCGC 1 cut(s) 845
Hin6I GCGC 1 cut(s) 843
HinP1I GCGC 1 cut(s) 843
HinfI GANTC 2 cut(s) 56, 562
HpaII CCGG 2 cut(s) 137, 885
HphI GGTGA 4 cut(s) 54, 196, 268, 871
Hpy166II GTNNAC 4 cut(s) 187, 367, 820, 985
Hpy188I TCNGA 1 cut(s) 52
Hpy188III TCNNGA 3 cut(s) 145, 169, 524
Hpy8I GTNNAC 4 cut(s) 187, 367, 820, 985
Hpy99I CGWCG 1 cut(s) 748
HpyAV CCTTC 1 cut(s) 285
HpyCH4III ACNGT 2 cut(s) 397, 477
HpyCH4IV ACGT 1 cut(s) 363
HpyF10VI GCNNNNNNNGC 6 cut(s) 89, 200, 209, 218, 749, 873
HpyF3I CTNAG 1 cut(s) 484
HpySE526I ACGT 1 cut(s) 363
HspAI GCGC 1 cut(s) 843
Ksp22I TGATCA 1 cut(s) 858
KspI CCGCGG 1 cut(s) 222
Kzo9I GATC 1 cut(s) 858
LmnI GCTCC 2 cut(s) 932, 957
Lsp1109I GCAGC 5 cut(s) 373, 583, 740, 902, 954
LweI GCATC 2 cut(s) 885, 969
MaeI CTAG 1 cut(s) 407
MaeII ACGT 1 cut(s) 363
MaeIII GTNAC 1 cut(s) 60
MalI GATC 1 cut(s) 860
MboI GATC 1 cut(s) 858
MboII GAAGA 2 cut(s) 184, 564
MhlI GDGCHC 3 cut(s) 144, 228, 250
MlsI TGGCCA 1 cut(s) 752
MluCI AATT 5 cut(s) 4, 40, 329, 374, 657
MluNI TGGCCA 1 cut(s) 752
MmeI TCCRAC 2 cut(s) 677, 922
MnlI CCTC 3 cut(s) 39, 420, 665
Mox20I TGGCCA 1 cut(s) 752
Mph1103I ATGCAT 1 cut(s) 94
MscI TGGCCA 1 cut(s) 752
MslI CAYNNNNRTG 3 cut(s) 67, 951, 990
Msp20I TGGCCA 1 cut(s) 752
MspA1I CMGCKG 1 cut(s) 221
MspI CCGG 2 cut(s) 137, 885
MspR9I CCNGG 3 cut(s) 138, 297, 885
Mva1269I GAATGC 1 cut(s) 893
MvaI CCWGG 1 cut(s) 297
MvnI CGCG 3 cut(s) 194, 221, 845
MwoI GCNNNNNNNGC 6 cut(s) 89, 200, 209, 218, 749, 873
NciI CCSGG 2 cut(s) 138, 885
NcoI CCATGG 3 cut(s) 114, 162, 936
NdeII GATC 1 cut(s) 858
NheI GCTAGC 1 cut(s) 406
NlaIV GGNNCC 2 cut(s) 160, 934
NmuCI GTSAC 1 cut(s) 60
NsiI ATGCAT 1 cut(s) 94
NspI RCATGY 2 cut(s) 259, 667
PceI AGGCCT 1 cut(s) 295
PciI ACATGT 2 cut(s) 255, 663
PcsI WCGNNNNNNNCGW 1 cut(s) 969
PctI GAATGC 1 cut(s) 893
PfeI GAWTC 2 cut(s) 56, 562
PkrI GCNGC 6 cut(s) 220, 388, 598, 730, 892, 944
Ppu21I YACGTR 1 cut(s) 364
PscI ACATGT 2 cut(s) 255, 663
Psp6I CCWGG 1 cut(s) 295
PspFI CCCAGC 1 cut(s) 410
PspGI CCWGG 1 cut(s) 295
PspN4I GGNNCC 2 cut(s) 160, 934
PspPI GGNCC 2 cut(s) 202, 611
RsaI GTAC 5 cut(s) 368, 459, 821, 849, 984
RsaNI GTAC 5 cut(s) 367, 458, 820, 848, 983
RseI CAYNNNNRTG 3 cut(s) 67, 951, 990
SacII CCGCGG 1 cut(s) 222
SatI GCNGC 6 cut(s) 219, 387, 597, 729, 891, 943
Sau3AI GATC 1 cut(s) 858
Sau96I GGNCC 2 cut(s) 202, 611
ScrFI CCNGG 3 cut(s) 138, 297, 885
SduI GDGCHC 3 cut(s) 144, 228, 250
SetI ASST 6 cut(s) 366, 412, 586, 676, 833, 980
SfaNI GCATC 2 cut(s) 885, 969
Sfr303I CCGCGG 1 cut(s) 222
SgrBI CCGCGG 1 cut(s) 222
SinI GGWCC 1 cut(s) 611
SmiMI CAYNNNNRTG 3 cut(s) 67, 951, 990
SmlI CTYRAG 1 cut(s) 524
SmoI CTYRAG 1 cut(s) 524
Sse9I AATT 5 cut(s) 4, 40, 329, 374, 657
SseBI AGGCCT 1 cut(s) 295
SsiI CCGC 6 cut(s) 182, 192, 219, 221, 552, 845
SspMI CTAG 1 cut(s) 407
StuI AGGCCT 1 cut(s) 295
StyD4I CCNGG 3 cut(s) 136, 295, 883
StyI CCWWGG 5 cut(s) 114, 162, 614, 936, 978
TaaI ACNGT 2 cut(s) 397, 477
TaiI ACGT 1 cut(s) 366
TaqI TCGA 1 cut(s) 972
TasI AATT 5 cut(s) 4, 40, 329, 374, 657
TatI WGTACW 2 cut(s) 366, 819
TauI GCSGC 1 cut(s) 221
TfiI GAWTC 2 cut(s) 56, 562
TscAI CASTG 3 cut(s) 480, 781, 868
TseFI GTSAC 1 cut(s) 60
TseI GCWGC 5 cut(s) 386, 596, 728, 890, 942
Tsp45I GTSAC 1 cut(s) 60
TspDTI ATGAA 4 cut(s) 17, 187, 446, 554
TspRI CASTG 3 cut(s) 480, 781, 868
VpaK11BI GGWCC 1 cut(s) 611
XapI RAATTY 1 cut(s) 40
XceI RCATGY 2 cut(s) 259, 667
XspI CTAG 1 cut(s) 407
Zsp2I ATGCAT 1 cut(s) 94
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.