Rh2DG248200

LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0000428
Physical Location & Seq
Forward (+)
25518966 .. 25521000
2035 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG248200.1

Sequence Viewer

Length: 1377 bp
ATGGCTTTAGAGTTGCCCGATCTTGTCATCCACCAAATCCTCCAATTCCTTCCCACTAAATCTGCTGTGCGCTTGAGCTTTCTTTCCAAGCAATGGGAAGGTGTATGGTCTTCGGCCCCAATAATAGATTTCGATGAGGGAGGTGAGGATGGAAACAACAACCTCAGCCAACATAAAAGGTTCGTCAAAAACATTCTGAGCAGGTATTTGGAATTCCTTGTTGAGAAAGAGAAAGAAAAACAAGGTTTAGAAAAATTCAAGCTTCACATGACAAGGTACTTATCTGGAGATGCTAGTATCGTAACCAAATTGTTGAGTTCTTCATTTGAGAGAGGCGTGAAAGAGTTAGATATCAACCTTAGAGTAAGTAAATCACAGTGGGATCATTGGAGTGGCATGAAAAACTACAACAACTTGTCCCCGATGGAGCTTATCAATGCAAAATCTGCGGCCCGTATGAAAAACTACTACTACTTTTCTCGGACGGCTCTTGCCAATGCAAAATCCTTGACTACTCTAAATCTAGGGTATGTTAGAATAAAGAACATTAGCTTTAAGGAATGCTCTACATCCACATGTCTTCTTCCCTCTTTGAAATCTATGTCTCTCAAGAAGGTCCACTTTGACAAGGAAGCTCTCTTCTACTTGATCCGTGAGTGCTCTTGCATCGAATATTTGTCAATAATTTCATGTTCTTTCAAAGACGGTGAATTTCGGGTCTCGAGTTCTAGTCTCAAGTCTTTGCAAGTTGAGCATTGCGACTCTTTTGCAATTCAAGTTGATGACGCTGTGAATCTTGAATCTCTCACAATTGTTTCAGTTATCGGTCCACCTCAACTTCCCAACTTCCATAGTATAATCTTGAAAGACTGCTTCAACTTGAAATGTTTGCACATATGTGCTCCGCAGGTGAAAGTATTCGATTTAGATGGATGCCATGATGTGAAAGGCACGATCGACGCTCCACTTCTAGATTCTATTACTTTCAAGGGTTACTTGAGTTCCAAACTTTCTCTCAAAGCTCCAAACTTGAGTATGACCCGCATCGTACTATTTGACAAATGGGACAGCGAAGCGGCCACTTTCAACAGCAGGCCATGGAACCATTTTACAACATTGAGCAATTTTCTTGAAGAATTTGGTAGCTCTAAAACTTTGGATCTTTATGTGAGTGATTTTAAGGCCCTTATGTTTCCAAAAAATTTCAGAAAGACCTTGTCTTCTCCAATATTCCCAAGTCTCAAAAAGCTTTATTTCTCGGTGAAGAATCCTCCAAGAAAGAAGAACAAAGAGTTGAGCGATTCGTTGTATTGGATGGCACCTGATGTGCTGTTCATTAAAAATCTACCCTGGAGAATCAGGCACAGACCTCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box LRR_2

Protein Analysis

458

Amino Acids

52.43

Weight (kDa)

9.16

Isoelectric Point (pI)

39.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 3 - 39 6.6e-08 F-box domain
LRR_At5g56370 PF24758 129 - 309 4.4e-15 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000428)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g09331 FvH4_1g09661 FvH4_1g21942 FvH4_2g05841 FvH4_2g06090 FvH4_3g02580 FvH4_3g02591 FvH4_6g51091 FvH4_7g18893 FvH4_7g20022 FvH4_7g20111
malus_domestica MD09G1117600.v1.1 MD17G1117300.v1.1 MD17G1286200.v1.1
prunus_persica Prupe.8G124500_v2.0.a1 Prupe.8G124700_v2.0.a1 Prupe.8G124700_v2.0.a1
pyrus_communis pycom17g10120
rosa_chinensis RchiOBHm_Chr1g0354511 RchiOBHm_Chr1g0360131 RchiOBHm_Chr1g0365181 RchiOBHm_Chr1g0365201 RchiOBHm_Chr2g0096151 RchiOBHm_Chr2g0113181 RchiOBHm_Chr6g0257131 RchiOBHm_Chr6g0257771 RchiOBHm_Chr6g0265441 RchiOBHm_Chr6g0265451 RchiOBHm_Chr6g0265471 RchiOBHm_Chr6g0265481
rosa_multiflora Rmu_co8204816.1_g000001 Rmu_co8281643.1_g000001 Rmu_sc0000146.1_g000019 Rmu_sc0000217.1_g000012 Rmu_sc0001530.1_g000020 Rmu_sc0003520.1_g000002 Rmu_sc0003983.1_g000008 Rmu_sc0004419.1_g000008 Rmu_sc0004448.1_g000035 Rmu_sc0004896.1_g000001 Rmu_sc0005996.1_g000016 Rmu_sc0006683.1_g000002 Rmu_sc0007813.1_g000006 Rmu_sc0008035.1_g000004 Rmu_sc0008490.1_g000002 Rmu_sc0008742.1_g000001 Rmu_sc0010908.1_g000002 Rmu_sc0010908.1_g000007 Rmu_sc0011315.1_g000015 Rmu_sc0013544.1_g000002 Rmu_sc0014962.1_g000001 Rmu_sc0015275.1_g000003 Rmu_sc0017847.1_g000006 Rmu_sc0024070.1_g000003 Rmu_sc0040696.1_g000001 Rmu_ssc0000066.1_g000007 Rmu_ssc0000368.1_g000042
rosa_roxburghii Rroxscaffold_175G00432410 Rroxscaffold_2G00129540 Rroxscaffold_2G00146070 Rroxscaffold_6G00430280 Rroxscaffold_7G00202650 Rroxscaffold_7G00208930
rosa_rugosa Rorug01G0302700 Rorug01G0302800 Rorug02G0116400 Rorug04G0099000 Rorug05G0568500 Rorug05G0575100
rosa_samantha Rh1CG237300 Rh1CG272100 Rh1CG307500 Rh2BG009200 Rh2DG103600 Rh2DG169700 Rh2DG169800 Rh2DG248200 Rh2DG609700 Rh3AG050300 Rh5AG024900 Rh5AG025400 Rh6AG092900 Rh6BG079600 Rh6BG083600 Rh6BG137900 Rh6BG138600 Rh6BG138900 Rh6BG139000 Rh6CG136100
rosa_wichuraiana Rw1G022220 Rw2G008030 Rw2G012930 Rw5G002340 Rw6G008000 Rw6G011990 Rw6G012000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 898
Acc36I ACCTGC 2 cut(s) 192, 898
AccB1I GGYRCC 1 cut(s) 1318
AccB7I CCANNNNNTGG 1 cut(s) 93
AciI CCGC 4 cut(s) 449, 905, 1042, 1076
AclWI GGATC 3 cut(s) 390, 643, 1167
AcoI YGGCCR 1 cut(s) 1077
AcsI RAATTY 5 cut(s) 212, 254, 710, 1136, 1201
AfaI GTAC 2 cut(s) 278, 1050
AfiI CCNNNNNNNGG 1 cut(s) 93
AflIII ACRYGT 1 cut(s) 575
AjnI CCWGG 1 cut(s) 1349
AleI CACNNNNGTG 1 cut(s) 897
AluBI AGCT 8 cut(s) 78, 262, 430, 552, 635, 1022, 1146, 1249
AluI AGCT 8 cut(s) 78, 262, 430, 552, 635, 1022, 1146, 1249
Alw21I GWGCWC 2 cut(s) 662, 904
Alw26I GTCTC 4 cut(s) 609, 724, 737, 1244
AlwI GGATC 3 cut(s) 390, 643, 1167
Ama87I CYCGRG 1 cut(s) 721
AoxI GGCC 5 cut(s) 114, 450, 1077, 1094, 1182
ApoI RAATTY 5 cut(s) 212, 254, 710, 1136, 1201
Asp700I GAANNNNTTC 1 cut(s) 917
AspLEI GCGC 1 cut(s) 72
AspS9I GGNCC 5 cut(s) 115, 451, 616, 827, 1183
AsuHPI GGTGA 4 cut(s) 155, 719, 922, 1273
AvaI CYCGRG 1 cut(s) 721
AvaII GGWCC 2 cut(s) 616, 827
BanI GGYRCC 1 cut(s) 1318
BbsI GAAGAC 3 cut(s) 102, 572, 1212
Bbv12I GWGCWC 2 cut(s) 662, 904
BbvCI CCTCAGC 1 cut(s) 164
BccI CCATC 4 cut(s) 143, 418, 923, 1309
BceAI ACGGC 1 cut(s) 501
BcgI CGANNNNNNTGC 2 cut(s) 749, 783
BciT130I CCWGG 1 cut(s) 1351
BcoDI GTCTC 4 cut(s) 609, 724, 737, 1244
BfaI CTAG 4 cut(s) 294, 524, 729, 971
BfuAI ACCTGC 2 cut(s) 192, 898
BisI GCNGC 2 cut(s) 450, 1077
BlsI GCNGC 2 cut(s) 451, 1078
Bme1390I CCNGG 1 cut(s) 1351
Bme18I GGWCC 2 cut(s) 616, 827
BmeT110I CYCGRG 1 cut(s) 721
BmgT120I GGNCC 5 cut(s) 115, 451, 616, 827, 1183
BmiI GGNNCC 3 cut(s) 117, 1103, 1320
BmrFI CCNGG 1 cut(s) 1351
BmsI GCATC 4 cut(s) 280, 675, 923, 1053
BpiI GAAGAC 3 cut(s) 102, 572, 1212
BpmI CTGGAG 2 cut(s) 306, 1372
Bpu10I CCTNAGC 1 cut(s) 164
BpuEI CTTGAG 5 cut(s) 94, 593, 719, 1018, 1051
BsaI GGTCTC 1 cut(s) 724
BsaJI CCNNGG 2 cut(s) 1097, 1349
Bsc4I CCNNNNNNNGG 1 cut(s) 93
Bse3DI GCAATG 2 cut(s) 98, 754
BseBI CCWGG 1 cut(s) 1351
BseDI CCNNGG 2 cut(s) 1097, 1349
BseGI GGATG 5 cut(s) 27, 154, 569, 938, 1320
BseLI CCNNNNNNNGG 1 cut(s) 93
BseMI GCAATG 2 cut(s) 98, 754
BseMII CTCAG 2 cut(s) 178, 188
Bsh1285I CGRYCG 1 cut(s) 957
BshFI GGCC 5 cut(s) 116, 452, 1079, 1096, 1184
BshNI GGYRCC 1 cut(s) 1318
BsiEI CGRYCG 1 cut(s) 957
BsiHKAI GWGCWC 2 cut(s) 662, 904
BsiHKCI CYCGRG 1 cut(s) 721
BslFI GGGAC 2 cut(s) 403, 1079
BslI CCNNNNNNNGG 1 cut(s) 93
BsmAI GTCTC 4 cut(s) 609, 724, 737, 1244
BsmFI GGGAC 2 cut(s) 403, 1079
BsmI GAATGC 1 cut(s) 566
BsnI GGCC 5 cut(s) 116, 452, 1079, 1096, 1184
Bso31I GGTCTC 1 cut(s) 724
BsoBI CYCGRG 1 cut(s) 721
Bsp1286I GDGCHC 2 cut(s) 662, 904
Bsp143I GATC 5 cut(s) 19, 382, 648, 954, 1159
Bsp19I CCATGG 1 cut(s) 1097
BspACI CCGC 4 cut(s) 449, 905, 1042, 1076
BspANI GGCC 5 cut(s) 116, 452, 1079, 1096, 1184
BspCNI CTCAG 2 cut(s) 177, 189
BspLI GGNNCC 3 cut(s) 117, 1103, 1320
BspMI ACCTGC 2 cut(s) 192, 898
BspPI GGATC 3 cut(s) 390, 643, 1167
BspT107I GGYRCC 1 cut(s) 1318
BspTNI GGTCTC 1 cut(s) 724
BsrDI GCAATG 2 cut(s) 98, 754
BssECI CCNNGG 2 cut(s) 1097, 1349
BssMI GATC 5 cut(s) 19, 382, 648, 954, 1159
BssT1I CCWWGG 1 cut(s) 1097
Bst2UI CCWGG 1 cut(s) 1351
Bst4CI ACNGT 2 cut(s) 378, 707
Bst6I CTCTTC 1 cut(s) 644
BstAPI GCANNNNNTGC 1 cut(s) 446
BstC8I GCNNGC 1 cut(s) 1094
BstDEI CTNAG 3 cut(s) 164, 197, 359
BstDSI CCRYGG 1 cut(s) 1097
BstF5I GGATG 5 cut(s) 27, 154, 569, 938, 1320
BstHHI GCGC 1 cut(s) 72
BstKTI GATC 5 cut(s) 22, 385, 651, 957, 1162
BstMAI GTCTC 4 cut(s) 609, 724, 737, 1244
BstMBI GATC 5 cut(s) 19, 382, 648, 954, 1159
BstMCI CGRYCG 1 cut(s) 957
BstMWI GCNNNNNNNGC 2 cut(s) 446, 751
BstNI CCWGG 1 cut(s) 1351
BstNSI RCATGY 1 cut(s) 579
BstSCI CCNGG 1 cut(s) 1349
BstV2I GAAGAC 3 cut(s) 102, 572, 1212
BstX2I RGATCY 1 cut(s) 1159
BstYI RGATCY 1 cut(s) 1159
BsuRI GGCC 5 cut(s) 116, 452, 1079, 1096, 1184
BtgI CCRYGG 1 cut(s) 1097
BtsCI GGATG 5 cut(s) 27, 154, 569, 938, 1320
BtsIMutI CAGTG 1 cut(s) 383
BveI ACCTGC 2 cut(s) 192, 898
Cac8I GCNNGC 1 cut(s) 1094
CfoI GCGC 1 cut(s) 72
Cfr13I GGNCC 5 cut(s) 115, 451, 616, 827, 1183
CseI GACGC 2 cut(s) 794, 968
Csp6I GTAC 2 cut(s) 277, 1049
CviAII CATG 6 cut(s) 268, 397, 576, 690, 938, 1098
CviQI GTAC 2 cut(s) 277, 1049
DdeI CTNAG 3 cut(s) 164, 197, 359
DpnI GATC 5 cut(s) 21, 384, 650, 956, 1161
DpnII GATC 5 cut(s) 19, 382, 648, 954, 1159
EaeI YGGCCR 1 cut(s) 1077
Eam1104I CTCTTC 1 cut(s) 644
EarI CTCTTC 1 cut(s) 644
Eco130I CCWWGG 1 cut(s) 1097
Eco31I GGTCTC 1 cut(s) 724
Eco32I GATATC 1 cut(s) 352
Eco47I GGWCC 2 cut(s) 616, 827
Eco88I CYCGRG 1 cut(s) 721
EcoO109I RGGNCCY 1 cut(s) 1183
EcoRI GAATTC 1 cut(s) 212
EcoRII CCWGG 1 cut(s) 1349
EcoRV GATATC 1 cut(s) 352
EcoT14I CCWWGG 1 cut(s) 1097
ErhI CCWWGG 1 cut(s) 1097
FaeI CATG 6 cut(s) 271, 400, 579, 693, 941, 1101
FalI AAGNNNNNCTT 4 cut(s) 605, 637, 980, 1012
FaqI GGGAC 2 cut(s) 403, 1079
FatI CATG 6 cut(s) 267, 396, 575, 689, 937, 1097
FauI CCCGC 1 cut(s) 1049
FauNDI CATATG 1 cut(s) 896
Fnu4HI GCNGC 2 cut(s) 450, 1077
FokI GGATG 5 cut(s) 14, 161, 556, 945, 1327
Fsp4HI GCNGC 2 cut(s) 450, 1077
FspBI CTAG 4 cut(s) 294, 524, 729, 971
GlaI GCGC 1 cut(s) 71
GluI GCNGC 2 cut(s) 450, 1077
GsuI CTGGAG 2 cut(s) 306, 1372
HaeIII GGCC 5 cut(s) 116, 452, 1079, 1096, 1184
HgaI GACGC 2 cut(s) 794, 968
HhaI GCGC 1 cut(s) 72
Hin1II CATG 6 cut(s) 271, 400, 579, 693, 941, 1101
Hin6I GCGC 1 cut(s) 70
HinP1I GCGC 1 cut(s) 70
HindIII AAGCTT 2 cut(s) 260, 1247
HinfI GANTC 7 cut(s) 761, 793, 800, 974, 1267, 1301, 1356
HphI GGTGA 4 cut(s) 155, 719, 922, 1273
Hpy166II GTNNAC 2 cut(s) 619, 830
Hpy188I TCNGA 3 cut(s) 198, 483, 1208
Hpy188III TCNNGA 7 cut(s) 285, 610, 721, 797, 862, 971, 1130
Hpy8I GTNNAC 2 cut(s) 619, 830
Hpy99I CGWCG 1 cut(s) 962
HpyAV CCTTC 3 cut(s) 59, 92, 607
HpyCH4III ACNGT 2 cut(s) 378, 707
HpyCH4V TGCA 6 cut(s) 440, 500, 666, 745, 770, 892
HpyF10VI GCNNNNNNNGC 2 cut(s) 446, 751
HpyF3I CTNAG 3 cut(s) 164, 197, 359
Hsp92II CATG 6 cut(s) 271, 400, 579, 693, 941, 1101
HspAI GCGC 1 cut(s) 70
Kzo9I GATC 5 cut(s) 19, 382, 648, 954, 1159
LmnI GCTCC 4 cut(s) 427, 907, 967, 1027
LpnPI CCDG 8 cut(s) 187, 270, 893, 1078, 1335, 1336, 1345, 1363
LweI GCATC 4 cut(s) 280, 675, 923, 1053
MaeI CTAG 4 cut(s) 294, 524, 729, 971
MaeIII GTNAC 2 cut(s) 301, 992
MalI GATC 5 cut(s) 21, 384, 650, 956, 1161
MboI GATC 5 cut(s) 19, 382, 648, 954, 1159
MboII GAAGA 9 cut(s) 102, 312, 572, 575, 631, 1145, 1212, 1276, 1294
MfeI CAATTG 1 cut(s) 810
MflI RGATCY 1 cut(s) 1159
MhlI GDGCHC 2 cut(s) 662, 904
MlyI GAGTC 1 cut(s) 755
MnlI CCTC 9 cut(s) 50, 130, 134, 139, 173, 326, 598, 843, 1281
MroXI GAANNNNTTC 1 cut(s) 917
MseI TTAA 3 cut(s) 555, 1179, 1338
MslI CAYNNNNRTG 3 cut(s) 390, 574, 897
MspR9I CCNGG 1 cut(s) 1351
MunI CAATTG 1 cut(s) 810
Mva1269I GAATGC 1 cut(s) 566
MvaI CCWGG 1 cut(s) 1351
MwoI GCNNNNNNNGC 2 cut(s) 446, 751
NcoI CCATGG 1 cut(s) 1097
NdeI CATATG 1 cut(s) 896
NdeII GATC 5 cut(s) 19, 382, 648, 954, 1159
NlaIII CATG 6 cut(s) 271, 400, 579, 693, 941, 1101
NlaIV GGNNCC 3 cut(s) 117, 1103, 1320
NspI RCATGY 1 cut(s) 579
OliI CACNNNNGTG 1 cut(s) 897
PaeR7I CTCGAG 1 cut(s) 721
PaqCI CACCTGC 1 cut(s) 898
PciI ACATGT 1 cut(s) 575
PctI GAATGC 1 cut(s) 566
PdmI GAANNNNTTC 1 cut(s) 917
PfeI GAWTC 6 cut(s) 793, 800, 974, 1267, 1301, 1356
PflFI GACNNNGTC 1 cut(s) 1216
PflMI CCANNNNNTGG 1 cut(s) 93
PkrI GCNGC 2 cut(s) 451, 1078
Ple19I CGATCG 1 cut(s) 957
PleI GAGTC 1 cut(s) 755
PpsI GAGTC 1 cut(s) 755
PscI ACATGT 1 cut(s) 575
Psp6I CCWGG 1 cut(s) 1349
PspGI CCWGG 1 cut(s) 1349
PspN4I GGNNCC 3 cut(s) 117, 1103, 1320
PspPI GGNCC 5 cut(s) 115, 451, 616, 827, 1183
PsuI RGATCY 1 cut(s) 1159
PsyI GACNNNGTC 1 cut(s) 1216
PvuI CGATCG 1 cut(s) 957
RsaI GTAC 2 cut(s) 278, 1050
RsaNI GTAC 2 cut(s) 277, 1049
RseI CAYNNNNRTG 3 cut(s) 390, 574, 897
SaqAI TTAA 3 cut(s) 555, 1179, 1338
SatI GCNGC 2 cut(s) 450, 1077
Sau3AI GATC 5 cut(s) 19, 382, 648, 954, 1159
Sau96I GGNCC 5 cut(s) 115, 451, 616, 827, 1183
SchI GAGTC 1 cut(s) 755
ScrFI CCNGG 1 cut(s) 1351
SduI GDGCHC 2 cut(s) 662, 904
SfaNI GCATC 4 cut(s) 280, 675, 923, 1053
Sfr274I CTCGAG 1 cut(s) 721
SinI GGWCC 2 cut(s) 616, 827
SlaI CTCGAG 1 cut(s) 721
SmiMI CAYNNNNRTG 3 cut(s) 390, 574, 897
SmlI CTYRAG 6 cut(s) 73, 608, 721, 734, 997, 1030
SmoI CTYRAG 6 cut(s) 73, 608, 721, 734, 997, 1030
SsiI CCGC 4 cut(s) 449, 905, 1042, 1076
SspI AATATT 2 cut(s) 674, 1230
SspMI CTAG 4 cut(s) 294, 524, 729, 971
StyD4I CCNGG 1 cut(s) 1349
StyI CCWWGG 1 cut(s) 1097
TaaI ACNGT 2 cut(s) 378, 707
TaqI TCGA 5 cut(s) 132, 669, 722, 921, 957
TaqII GACCGA 1 cut(s) 815
TauI GCSGC 2 cut(s) 452, 1079
TfiI GAWTC 6 cut(s) 793, 800, 974, 1267, 1301, 1356
Tru1I TTAA 3 cut(s) 555, 1179, 1338
Tru9I TTAA 3 cut(s) 555, 1179, 1338
TscAI CASTG 1 cut(s) 383
TspDTI ATGAA 5 cut(s) 312, 413, 473, 678, 1324
TspGWI ACGGA 1 cut(s) 641
TspRI CASTG 1 cut(s) 383
Tth111I GACNNNGTC 1 cut(s) 1216
Van91I CCANNNNNTGG 1 cut(s) 93
VpaK11BI GGWCC 2 cut(s) 616, 827
XapI RAATTY 5 cut(s) 212, 254, 710, 1136, 1201
XbaI TCTAGA 1 cut(s) 970
XceI RCATGY 1 cut(s) 579
XhoI CTCGAG 1 cut(s) 721
XmnI GAANNNNTTC 1 cut(s) 917
XspI CTAG 4 cut(s) 294, 524, 729, 971
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.