Rw5G002340

LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000428
Physical Location & Seq
Forward (+)
2005968 .. 2007326
1359 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G002340.1

Sequence Viewer

Length: 1329 bp
ATGTCTAACGTGGTCATGTCACAAGGAGAAACAAGCCGGAAACGGATGAAGAGCTCCTCAAAGCAAGACGAAACAAAGGCCGTCTTTGACGTCAACACAATGAGGCCTTCTTTGCCTCACCTTGTTATACATCGAATACTCGAATTGCTTCCCACTAAAGCCGCTTTCCGAATGAGCTTTCTTTCCAAGCAATGGGAAGGTGTATGGTCCTTGAGCCCTGTAATAGATTTCGATGAGTCTGGAAAGACCGATGACGACGACGTGATTTTTCATCAACTAGTTCAACACAAAAACTTCATCCACATTTTGCAAACGTATTTGGAATTCCGTGAGAAAGACAGGACGACACCAGTTTTGGATAAGTTCAAACTTCACATGAGGAGATACTTATTTAGAGATTCTGCTATCGTAGATAAGTGGTTGAGTTTTGCATTTGAGAGAAGCGTCAAGGAGGTAGATCTTAGCCTTGATATTGCTTGGCATAAACGGTTGGAATTTTACTATTGCATATCGCCAATGAGTTTTCTTAATGCAAAATCCATAACTACTTTGAATTTGGAGTTTGTGAGAATACAACACGTGTATTGTCCTGAGCTTAAAGATACAAGAGTTCTTCCTTCTTTGAGAAACATGTCTCTGAAAAGTGTCCACTTCAACTGCCGGGCTCCTCTGGGCCTAATTCGGGAGTGCCCTTCCATCGAATGTCTGTCATTAACTTCATGTTACTTTGAAGACTTGGCATTTTATTTTTCAAGTTCTAGTCTCAAATCTTTGAAAGCTACGGATTGCGATGCTCATCTTATTAAGGTGGACGAAGCTGTGAATCTTGAACACTTTACATTTTCGTCAAAATTCCAGCTCAAGAGTATGATTTTGAAAAAATGCTTCAACTTGAAATATATCAACATCCATGCTCAGCACCTCGAAAAACTTACTTTGCATGGATGCCATGAGAGTCTGAAGGCCACTATCTATACTCCGCGTCTTCTTCATTTGAAGTTTCGTGGTTTTCTAAAATCCAAAGTGTCTTTGAAATGTCCATATTTAAGGACAACCGTCATCTTACTTTGGGACATTTTTGATGGAGAATCAGATTTTCTAGCATTGAGAGATTTCTTTAAAGCATTTGATGGCTCCGACAGTATAACCCTCTATGTTCATGATATTAAGACCTTCTCGTCACCATTGTGTAAGATCAGAGAGCTCATGATGATCCTATTGTCAAATCCTCCAACAAAGGCAGAAGATATTTCCGACTTGAATGATTCCTTGACTTGGATGGCACCTTCTGCAAGTAGAAACTGGATGGCACCTTCTGAAGCTATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box LRR_2

Protein Analysis

442

Amino Acids

51.32

Weight (kDa)

8.62

Isoelectric Point (pI)

49.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_At1g61320_AtMIF1 PF23622 121 - 387 4.1e-09 At1g61320/AtMIF1, LRR domain
LRR_At5g56370 PF24758 137 - 311 9.3e-13 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000428)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g09331 FvH4_1g09661 FvH4_1g21942 FvH4_2g05841 FvH4_2g06090 FvH4_3g02580 FvH4_3g02591 FvH4_6g51091 FvH4_7g18893 FvH4_7g20022 FvH4_7g20111
malus_domestica MD09G1117600.v1.1 MD17G1117300.v1.1 MD17G1286200.v1.1
prunus_persica Prupe.8G124500_v2.0.a1 Prupe.8G124700_v2.0.a1 Prupe.8G124700_v2.0.a1
pyrus_communis pycom17g10120
rosa_chinensis RchiOBHm_Chr1g0354511 RchiOBHm_Chr1g0360131 RchiOBHm_Chr1g0365181 RchiOBHm_Chr1g0365201 RchiOBHm_Chr2g0096151 RchiOBHm_Chr2g0113181 RchiOBHm_Chr6g0257131 RchiOBHm_Chr6g0257771 RchiOBHm_Chr6g0265441 RchiOBHm_Chr6g0265451 RchiOBHm_Chr6g0265471 RchiOBHm_Chr6g0265481
rosa_multiflora Rmu_co8204816.1_g000001 Rmu_co8281643.1_g000001 Rmu_sc0000146.1_g000019 Rmu_sc0000217.1_g000012 Rmu_sc0001530.1_g000020 Rmu_sc0003520.1_g000002 Rmu_sc0003983.1_g000008 Rmu_sc0004419.1_g000008 Rmu_sc0004448.1_g000035 Rmu_sc0004896.1_g000001 Rmu_sc0005996.1_g000016 Rmu_sc0006683.1_g000002 Rmu_sc0007813.1_g000006 Rmu_sc0008035.1_g000004 Rmu_sc0008490.1_g000002 Rmu_sc0008742.1_g000001 Rmu_sc0010908.1_g000002 Rmu_sc0010908.1_g000007 Rmu_sc0011315.1_g000015 Rmu_sc0013544.1_g000002 Rmu_sc0014962.1_g000001 Rmu_sc0015275.1_g000003 Rmu_sc0017847.1_g000006 Rmu_sc0024070.1_g000003 Rmu_sc0040696.1_g000001 Rmu_ssc0000066.1_g000007 Rmu_ssc0000368.1_g000042
rosa_roxburghii Rroxscaffold_175G00432410 Rroxscaffold_2G00129540 Rroxscaffold_2G00146070 Rroxscaffold_6G00430280 Rroxscaffold_7G00202650 Rroxscaffold_7G00208930
rosa_rugosa Rorug01G0302700 Rorug01G0302800 Rorug02G0116400 Rorug04G0099000 Rorug05G0568500 Rorug05G0575100
rosa_samantha Rh1CG237300 Rh1CG272100 Rh1CG307500 Rh2BG009200 Rh2DG103600 Rh2DG169700 Rh2DG169800 Rh2DG248200 Rh2DG609700 Rh3AG050300 Rh5AG024900 Rh5AG025400 Rh6AG092900 Rh6BG079600 Rh6BG083600 Rh6BG137900 Rh6BG138600 Rh6BG138900 Rh6BG139000 Rh6CG136100
rosa_wichuraiana Rw1G022220 Rw2G008030 Rw2G012930 Rw5G002340 Rw6G008000 Rw6G011990 Rw6G012000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1177
AatII GACGTC 1 cut(s) 93
AccB1I GGYRCC 2 cut(s) 1282, 1309
AccB7I CCANNNNNTGG 1 cut(s) 192
AccII CGCG 1 cut(s) 982
AciI CCGC 2 cut(s) 162, 980
AclWI GGATC 1 cut(s) 1207
AcsI RAATTY 4 cut(s) 323, 494, 553, 851
AcuI CTGAAG 1 cut(s) 980
AcvI CACGTG 1 cut(s) 580
AcyI GRCGYC 1 cut(s) 90
AfiI CCNNNNNNNGG 3 cut(s) 192, 682, 1275
AflIII ACRYGT 3 cut(s) 577, 579, 630
AhlI ACTAGT 1 cut(s) 277
AjiI CACGTC 1 cut(s) 262
AleI CACNNNNGTG 1 cut(s) 1186
AluBI AGCT 8 cut(s) 54, 177, 595, 779, 818, 859, 1204, 1322
AluI AGCT 8 cut(s) 54, 177, 595, 779, 818, 859, 1204, 1322
Alw21I GWGCWC 2 cut(s) 56, 1206
Alw26I GTCTC 2 cut(s) 639, 767
AlwI GGATC 1 cut(s) 1207
AoxI GGCC 4 cut(s) 78, 104, 673, 963
ApoI RAATTY 4 cut(s) 323, 494, 553, 851
ArsI GACNNNNNNTTYG 2 cut(s) 337, 369
Asp700I GAANNNNTTC 1 cut(s) 147
AspS9I GGNCC 2 cut(s) 207, 673
AsuC2I CCSGG 1 cut(s) 662
AsuHPI GGTGA 2 cut(s) 110, 1173
AvaII GGWCC 1 cut(s) 207
BaeGI GKGCMC 1 cut(s) 692
BaeI ACNNNNGTAYC 2 cut(s) 594, 627
BanI GGYRCC 2 cut(s) 1282, 1309
BanII GRGCYC 4 cut(s) 56, 218, 667, 1206
BbrPI CACGTG 1 cut(s) 580
BbsI GAAGAC 2 cut(s) 738, 977
Bbv12I GWGCWC 2 cut(s) 56, 1206
BccI CCATC 5 cut(s) 704, 1076, 1124, 1273, 1300
BceAI ACGGC 1 cut(s) 65
BcnI CCSGG 1 cut(s) 662
BcoDI GTCTC 2 cut(s) 639, 767
BcuI ACTAGT 1 cut(s) 277
BfaI CTAG 3 cut(s) 278, 759, 1100
BglII AGATCT 1 cut(s) 457
BisI GCNGC 1 cut(s) 162
BlpI GCTNAGC 1 cut(s) 915
BlsI GCNGC 1 cut(s) 163
Bme1390I CCNGG 1 cut(s) 662
Bme18I GGWCC 1 cut(s) 207
BmgBI CACGTC 1 cut(s) 262
BmgT120I GGNCC 2 cut(s) 207, 673
BmiI GGNNCC 4 cut(s) 666, 1135, 1284, 1311
BmrFI CCNGG 1 cut(s) 662
BmsI GCATC 2 cut(s) 781, 935
BoxI GACNNNNGTC 1 cut(s) 1055
BpiI GAAGAC 2 cut(s) 738, 977
Bpu10I CCTNAGC 1 cut(s) 591
Bpu1102I GCTNAGC 1 cut(s) 915
BpuEI CTTGAG 2 cut(s) 232, 845
BpuMI CCSGG 1 cut(s) 662
BsaAI YACGTR 1 cut(s) 580
BsaBI GATNNNNATC 1 cut(s) 795
BsaHI GRCGYC 1 cut(s) 90
Bsc4I CCNNNNNNNGG 3 cut(s) 192, 682, 1275
Bse1I ACTGG 2 cut(s) 350, 1307
Bse3DI GCAATG 1 cut(s) 197
Bse8I GATNNNNATC 1 cut(s) 795
BseGI GGATG 6 cut(s) 51, 297, 906, 950, 1284, 1311
BseJI GATNNNNATC 1 cut(s) 795
BseLI CCNNNNNNNGG 3 cut(s) 192, 682, 1275
BseMI GCAATG 1 cut(s) 197
BseMII CTCAG 2 cut(s) 582, 929
BseNI ACTGG 2 cut(s) 350, 1307
BseRI GAGGAG 3 cut(s) 46, 394, 657
BseSI GKGCMC 1 cut(s) 692
Bsh1236I CGCG 1 cut(s) 982
BshFI GGCC 4 cut(s) 80, 106, 675, 965
BshNI GGYRCC 2 cut(s) 1282, 1309
BsiHKAI GWGCWC 2 cut(s) 56, 1206
BsiSI CCGG 2 cut(s) 37, 661
BslFI GGGAC 1 cut(s) 1085
BslI CCNNNNNNNGG 3 cut(s) 192, 682, 1275
BsmAI GTCTC 2 cut(s) 639, 767
BsmFI GGGAC 1 cut(s) 1085
BsnI GGCC 4 cut(s) 80, 106, 675, 965
Bsp1286I GDGCHC 5 cut(s) 56, 218, 667, 692, 1206
Bsp143I GATC 3 cut(s) 457, 1194, 1212
Bsp1720I GCTNAGC 1 cut(s) 915
BspACI CCGC 2 cut(s) 162, 980
BspANI GGCC 4 cut(s) 80, 106, 675, 965
BspCNI CTCAG 2 cut(s) 583, 928
BspFNI CGCG 1 cut(s) 982
BspHI TCATGA 2 cut(s) 1159, 1206
BspLI GGNNCC 4 cut(s) 666, 1135, 1284, 1311
BspPI GGATC 1 cut(s) 1207
BspQI GCTCTTC 1 cut(s) 44
BspT107I GGYRCC 2 cut(s) 1282, 1309
BsrDI GCAATG 1 cut(s) 197
BsrI ACTGG 2 cut(s) 350, 1307
BssMI GATC 3 cut(s) 457, 1194, 1212
BssNI GRCGYC 1 cut(s) 90
Bst4CI ACNGT 3 cut(s) 489, 1057, 1142
Bst6I CTCTTC 1 cut(s) 44
BstACI GRCGYC 1 cut(s) 90
BstAPI GCANNNNNTGC 1 cut(s) 1289
BstBAI YACGTR 1 cut(s) 580
BstDEI CTNAG 3 cut(s) 461, 591, 915
BstF5I GGATG 6 cut(s) 51, 297, 906, 950, 1284, 1311
BstFNI CGCG 1 cut(s) 982
BstKTI GATC 3 cut(s) 460, 1197, 1215
BstMAI GTCTC 2 cut(s) 639, 767
BstMBI GATC 3 cut(s) 457, 1194, 1212
BstMWI GCNNNNNNNGC 2 cut(s) 112, 1289
BstNSI RCATGY 1 cut(s) 634
BstPAI GACNNNNGTC 1 cut(s) 1055
BstSCI CCNGG 1 cut(s) 660
BstSLI GKGCMC 1 cut(s) 692
BstUI CGCG 1 cut(s) 982
BstV2I GAAGAC 2 cut(s) 738, 977
BstX2I RGATCY 1 cut(s) 457
BstYI RGATCY 1 cut(s) 457
BsuRI GGCC 4 cut(s) 80, 106, 675, 965
BtgZI GCGATG 1 cut(s) 804
BtrI CACGTC 1 cut(s) 262
BtsCI GGATG 6 cut(s) 51, 297, 906, 950, 1284, 1311
CciI TCATGA 2 cut(s) 1159, 1206
Cfr13I GGNCC 2 cut(s) 207, 673
CseI GACGC 2 cut(s) 433, 971
CviAII CATG 9 cut(s) 16, 376, 631, 720, 911, 941, 950, 1160, 1207
DdeI CTNAG 3 cut(s) 461, 591, 915
DpnI GATC 3 cut(s) 459, 1196, 1214
DpnII GATC 3 cut(s) 457, 1194, 1212
DraI TTTAAA 1 cut(s) 1120
DrdI GACNNNNNNGTC 1 cut(s) 1177
DseDI GACNNNNNNGTC 1 cut(s) 1177
Eam1104I CTCTTC 1 cut(s) 44
EarI CTCTTC 1 cut(s) 44
Ecl136II GAGCTC 2 cut(s) 54, 1204
Eco147I AGGCCT 1 cut(s) 106
Eco24I GRGCYC 4 cut(s) 56, 218, 667, 1206
Eco47I GGWCC 1 cut(s) 207
Eco53kI GAGCTC 2 cut(s) 54, 1204
Eco57I CTGAAG 1 cut(s) 980
Eco72I CACGTG 1 cut(s) 580
EcoICRI GAGCTC 2 cut(s) 54, 1204
EcoRI GAATTC 1 cut(s) 323
EcoT38I GRGCYC 4 cut(s) 56, 218, 667, 1206
FaeI CATG 9 cut(s) 19, 379, 634, 723, 914, 944, 953, 1163, 1210
FalI AAGNNNNNCTT 2 cut(s) 68, 100
FaqI GGGAC 1 cut(s) 1085
FatI CATG 9 cut(s) 15, 375, 630, 719, 910, 940, 949, 1159, 1206
Fnu4HI GCNGC 1 cut(s) 162
FokI GGATG 6 cut(s) 58, 284, 893, 957, 1291, 1318
FriOI GRGCYC 4 cut(s) 56, 218, 667, 1206
Fsp4HI GCNGC 1 cut(s) 162
FspBI CTAG 3 cut(s) 278, 759, 1100
GluI GCNGC 1 cut(s) 162
HaeIII GGCC 4 cut(s) 80, 106, 675, 965
HapII CCGG 2 cut(s) 37, 661
HgaI GACGC 2 cut(s) 433, 971
Hin1I GRCGYC 1 cut(s) 90
Hin1II CATG 9 cut(s) 19, 379, 634, 723, 914, 944, 953, 1163, 1210
HincII GTYRAC 1 cut(s) 94
HindII GTYRAC 1 cut(s) 94
HinfI GANTC 6 cut(s) 236, 398, 823, 955, 1088, 1265
HpaII CCGG 2 cut(s) 37, 661
HphI GGTGA 2 cut(s) 110, 1173
Hpy166II GTNNAC 3 cut(s) 94, 649, 811
Hpy188I TCNGA 8 cut(s) 170, 639, 960, 1093, 1138, 1199, 1255, 1318
Hpy188III TCNNGA 7 cut(s) 240, 590, 683, 827, 862, 1160, 1207
Hpy8I GTNNAC 3 cut(s) 94, 649, 811
Hpy99I CGWCG 2 cut(s) 260, 263
HpyAV CCTTC 8 cut(s) 117, 191, 627, 702, 955, 1183, 1296, 1323
HpyCH4III ACNGT 3 cut(s) 489, 1057, 1142
HpyCH4IV ACGT 5 cut(s) 9, 90, 261, 314, 579
HpyCH4V TGCA 6 cut(s) 310, 431, 507, 533, 940, 1292
HpyF10VI GCNNNNNNNGC 2 cut(s) 112, 1289
HpyF3I CTNAG 3 cut(s) 461, 591, 915
HpySE526I ACGT 5 cut(s) 9, 90, 261, 314, 579
Hsp92I GRCGYC 1 cut(s) 90
Hsp92II CATG 9 cut(s) 19, 379, 634, 723, 914, 944, 953, 1163, 1210
Kzo9I GATC 3 cut(s) 457, 1194, 1212
LguI GCTCTTC 1 cut(s) 44
LmnI GCTCC 3 cut(s) 59, 670, 1139
LweI GCATC 2 cut(s) 781, 935
MaeI CTAG 3 cut(s) 278, 759, 1100
MaeII ACGT 5 cut(s) 9, 90, 261, 314, 579
MaeIII GTNAC 3 cut(s) 18, 722, 1179
MalI GATC 3 cut(s) 459, 1196, 1214
MboI GATC 3 cut(s) 457, 1194, 1212
MboII GAAGA 6 cut(s) 61, 605, 743, 977, 980, 1256
MflI RGATCY 1 cut(s) 457
MhlI GDGCHC 5 cut(s) 56, 218, 667, 692, 1206
MluCI AATT 6 cut(s) 143, 323, 494, 553, 678, 851
MlyI GAGTC 2 cut(s) 245, 964
MmeI TCCRAC 4 cut(s) 471, 1161, 1256, 1278
MnlI CCTC 9 cut(s) 67, 96, 126, 372, 445, 678, 932, 1160, 1239
MroXI GAANNNNTTC 1 cut(s) 147
MseI TTAA 7 cut(s) 528, 597, 713, 804, 1046, 1119, 1167
MslI CAYNNNNRTG 1 cut(s) 1186
MspI CCGG 2 cut(s) 37, 661
MspR9I CCNGG 1 cut(s) 662
MvnI CGCG 1 cut(s) 982
MwoI GCNNNNNNNGC 2 cut(s) 112, 1289
NciI CCSGG 1 cut(s) 662
NdeII GATC 3 cut(s) 457, 1194, 1212
NlaIII CATG 9 cut(s) 19, 379, 634, 723, 914, 944, 953, 1163, 1210
NlaIV GGNNCC 4 cut(s) 666, 1135, 1284, 1311
NmuCI GTSAC 2 cut(s) 18, 1179
NspI RCATGY 1 cut(s) 634
OliI CACNNNNGTG 1 cut(s) 1186
PagI TCATGA 2 cut(s) 1159, 1206
PceI AGGCCT 1 cut(s) 106
PciI ACATGT 1 cut(s) 630
PciSI GCTCTTC 1 cut(s) 44
PdmI GAANNNNTTC 1 cut(s) 147
PfeI GAWTC 4 cut(s) 398, 823, 1088, 1265
PflMI CCANNNNNTGG 1 cut(s) 192
PkrI GCNGC 1 cut(s) 163
PleI GAGTC 2 cut(s) 244, 963
PmaCI CACGTG 1 cut(s) 580
PmlI CACGTG 1 cut(s) 580
PpsI GAGTC 2 cut(s) 244, 963
Ppu21I YACGTR 1 cut(s) 580
PscI ACATGT 1 cut(s) 630
PshAI GACNNNNGTC 1 cut(s) 1055
Psp124BI GAGCTC 2 cut(s) 56, 1206
PspCI CACGTG 1 cut(s) 580
PspN4I GGNNCC 4 cut(s) 666, 1135, 1284, 1311
PspPI GGNCC 2 cut(s) 207, 673
PsuI RGATCY 1 cut(s) 457
RseI CAYNNNNRTG 1 cut(s) 1186
SacI GAGCTC 2 cut(s) 56, 1206
SapI GCTCTTC 1 cut(s) 44
SaqAI TTAA 7 cut(s) 528, 597, 713, 804, 1046, 1119, 1167
SatI GCNGC 1 cut(s) 162
Sau3AI GATC 3 cut(s) 457, 1194, 1212
Sau96I GGNCC 2 cut(s) 207, 673
SchI GAGTC 2 cut(s) 245, 964
ScrFI CCNGG 1 cut(s) 662
SduI GDGCHC 5 cut(s) 56, 218, 667, 692, 1206
SfaNI GCATC 2 cut(s) 781, 935
SinI GGWCC 1 cut(s) 207
SmiMI CAYNNNNRTG 1 cut(s) 1186
SmlI CTYRAG 2 cut(s) 211, 860
SmoI CTYRAG 2 cut(s) 211, 860
SpeI ACTAGT 1 cut(s) 277
Sse9I AATT 6 cut(s) 143, 323, 494, 553, 678, 851
SseBI AGGCCT 1 cut(s) 106
SsiI CCGC 2 cut(s) 162, 980
SspMI CTAG 3 cut(s) 278, 759, 1100
SstI GAGCTC 2 cut(s) 56, 1206
StuI AGGCCT 1 cut(s) 106
StyD4I CCNGG 1 cut(s) 660
TaaI ACNGT 3 cut(s) 489, 1057, 1142
TaiI ACGT 5 cut(s) 12, 93, 264, 317, 582
TaqI TCGA 5 cut(s) 133, 141, 231, 699, 924
TaqII GACCGA 1 cut(s) 263
TasI AATT 6 cut(s) 143, 323, 494, 553, 678, 851
TauI GCSGC 1 cut(s) 164
TfiI GAWTC 4 cut(s) 398, 823, 1088, 1265
Tru1I TTAA 7 cut(s) 528, 597, 713, 804, 1046, 1119, 1167
Tru9I TTAA 7 cut(s) 528, 597, 713, 804, 1046, 1119, 1167
TseFI GTSAC 2 cut(s) 18, 1179
Tsp45I GTSAC 2 cut(s) 18, 1179
TspDTI ATGAA 6 cut(s) 62, 260, 286, 708, 980, 1148
TspGWI ACGGA 3 cut(s) 58, 317, 797
Van91I CCANNNNNTGG 1 cut(s) 192
VpaK11BI GGWCC 1 cut(s) 207
XapI RAATTY 4 cut(s) 323, 494, 553, 851
XceI RCATGY 1 cut(s) 634
XmnI GAANNNNTTC 1 cut(s) 147
XspI CTAG 3 cut(s) 278, 759, 1100
ZraI GACGTC 1 cut(s) 91
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.