Rw2G008030

F-box FBD LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000428
Physical Location & Seq
Reverse (-)
7873783 .. 7875252
1470 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G008030.1

Sequence Viewer

Length: 1227 bp
ATGGCTGTGGACACATTGCTACCTCACCTTCCAGACCTTATTGTTCACAAGATTTTCCGACTCATTCCCACTAAACTTGCCATACGCTCAACCATTGTTTCTAAACAATGGGCAAGTGTGTGGTCTTTGGGAGTCCCCCTCTTAGATTTCGATGACGAGGGGGACTCTACTGATCATGATGGTGACAATCTCGTCCGCAGGAAGCTGTTGTTCATTGATTTTGTGGAAAGCTGTATGAAACATTGTGGGAAAAAGAAGCTCTTGGAGTTGGATAGTTTCAGGCTTCGAATGAGATATGATTCTGAGTTAATAGGCGCGACAAGAATAGACAAGTGGTTTAGTTTTGTGGTTGATAGATGCGTCAAAGAGTTAGATATCAGCATTCAAAGAAAAGATGACAATGATCCGGTCAAAAGCACATACTACTGCATACCTCATACCATTCTCAATGCTCAATTTCTTACTAGTCTGAATCTGGAGCATGTGAGAATACAGGAGAGTATTGATCTGATAAGCCTTCCGTTGTTGAAAACCATGTCCCTAAACACTGTGCTGTTTGATTGCCGCATGCATTCGGATAGATCACTCTGGATGGGTTGTCCTTCCATCGAGCATTTGTCATTAACTTCGTGTTCTTTTAGCTCATTACAGTGTCCGACGATCACTGTCATAAATTCCAGTCTTAAATTCTTGCAGGTTATGTATTGCAAGTGTGTGTCTTTTCAAGTTAGAGCTGAGAATCTTGAATCTGTTATATTCACTTCAGAATTTTCTCAACTTCATTCCATCTGCTTGCTTAATGAATGCGTCAAGTTGAAATATATCAGCATTTATGCTCAGCATGCGAAGATTTGTGAAATTCCCGGAACATGTCGAGATAATGTGAAGGCCAATATTGATATTCCAAATCTCCGAGTTTTTGGCTACGATGATTTTCTTCAAAACTTTGATTGCTTCGATAAATTAGAGCTAGATATTCGTCATGATGCCATTGCAGAGGTACTCACGTTTTCAGAAAATTTCAGGAAGACCTTCTCACCACCATTGCCTAATATCAAGTATGTTACAATCTCGATTGTACGTCCTCGTTGTCCGCAGACAGTGGCCAGAGTTTCTGCCTTGATAAACTCTTTGCATTGGATGGCACCATCTGCAACAATCACATTAAATTACTGGGACTATGGACCGCCTGATTCTTCAGGGGAATCTTCAGGCGAAGATGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

408

Amino Acids

46.65

Weight (kDa)

5.86

Isoelectric Point (pI)

41.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_At1g61320_AtMIF1 PF23622 93 - 281 1.7e-07 At1g61320/AtMIF1, LRR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000428)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g09331 FvH4_1g09661 FvH4_1g21942 FvH4_2g05841 FvH4_2g06090 FvH4_3g02580 FvH4_3g02591 FvH4_6g51091 FvH4_7g18893 FvH4_7g20022 FvH4_7g20111
malus_domestica MD09G1117600.v1.1 MD17G1117300.v1.1 MD17G1286200.v1.1
prunus_persica Prupe.8G124500_v2.0.a1 Prupe.8G124700_v2.0.a1 Prupe.8G124700_v2.0.a1
pyrus_communis pycom17g10120
rosa_chinensis RchiOBHm_Chr1g0354511 RchiOBHm_Chr1g0360131 RchiOBHm_Chr1g0365181 RchiOBHm_Chr1g0365201 RchiOBHm_Chr2g0096151 RchiOBHm_Chr2g0113181 RchiOBHm_Chr6g0257131 RchiOBHm_Chr6g0257771 RchiOBHm_Chr6g0265441 RchiOBHm_Chr6g0265451 RchiOBHm_Chr6g0265471 RchiOBHm_Chr6g0265481
rosa_multiflora Rmu_co8204816.1_g000001 Rmu_co8281643.1_g000001 Rmu_sc0000146.1_g000019 Rmu_sc0000217.1_g000012 Rmu_sc0001530.1_g000020 Rmu_sc0003520.1_g000002 Rmu_sc0003983.1_g000008 Rmu_sc0004419.1_g000008 Rmu_sc0004448.1_g000035 Rmu_sc0004896.1_g000001 Rmu_sc0005996.1_g000016 Rmu_sc0006683.1_g000002 Rmu_sc0007813.1_g000006 Rmu_sc0008035.1_g000004 Rmu_sc0008490.1_g000002 Rmu_sc0008742.1_g000001 Rmu_sc0010908.1_g000002 Rmu_sc0010908.1_g000007 Rmu_sc0011315.1_g000015 Rmu_sc0013544.1_g000002 Rmu_sc0014962.1_g000001 Rmu_sc0015275.1_g000003 Rmu_sc0017847.1_g000006 Rmu_sc0024070.1_g000003 Rmu_sc0040696.1_g000001 Rmu_ssc0000066.1_g000007 Rmu_ssc0000368.1_g000042
rosa_roxburghii Rroxscaffold_175G00432410 Rroxscaffold_2G00129540 Rroxscaffold_2G00146070 Rroxscaffold_6G00430280 Rroxscaffold_7G00202650 Rroxscaffold_7G00208930
rosa_rugosa Rorug01G0302700 Rorug01G0302800 Rorug02G0116400 Rorug04G0099000 Rorug05G0568500 Rorug05G0575100
rosa_samantha Rh1CG237300 Rh1CG272100 Rh1CG307500 Rh2BG009200 Rh2DG103600 Rh2DG169700 Rh2DG169800 Rh2DG248200 Rh2DG609700 Rh3AG050300 Rh5AG024900 Rh5AG025400 Rh6AG092900 Rh6BG079600 Rh6BG083600 Rh6BG137900 Rh6BG138600 Rh6BG138900 Rh6BG139000 Rh6CG136100
rosa_wichuraiana Rw1G022220 Rw2G008030 Rw2G012930 Rw5G002340 Rw6G008000 Rw6G011990 Rw6G012000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 191
Acc36I ACCTGC 1 cut(s) 685
AccB1I GGYRCC 1 cut(s) 1144
AccII CGCG 1 cut(s) 317
AciI CCGC 4 cut(s) 196, 565, 1094, 1187
AclWI GGATC 1 cut(s) 398
AcoI YGGCCR 1 cut(s) 1104
AcsI RAATTY 5 cut(s) 673, 686, 767, 858, 1018
AcuI CTGAAG 3 cut(s) 747, 1182, 1194
AfaI GTAC 2 cut(s) 1002, 1080
AflIII ACRYGT 1 cut(s) 869
AgsI TTSAA 6 cut(s) 386, 529, 725, 746, 817, 941
AhlI ACTAGT 1 cut(s) 464
AluBI AGCT 6 cut(s) 205, 231, 259, 642, 734, 970
AluI AGCT 6 cut(s) 205, 231, 259, 642, 734, 970
AlwI GGATC 1 cut(s) 398
AoxI GGCC 2 cut(s) 888, 1104
ApoI RAATTY 5 cut(s) 673, 686, 767, 858, 1018
Asp700I GAANNNNTTC 1 cut(s) 1031
AspLEI GCGC 1 cut(s) 317
AspS9I GGNCC 1 cut(s) 1184
AsuC2I CCSGG 1 cut(s) 864
AsuHPI GGTGA 3 cut(s) 17, 194, 1029
AsuII TTCGAA 1 cut(s) 286
AvaII GGWCC 1 cut(s) 1184
BaeI ACNNNNGTAYC 2 cut(s) 992, 1025
BalI TGGCCA 1 cut(s) 1106
BanI GGYRCC 1 cut(s) 1144
BbsI GAAGAC 1 cut(s) 1034
BccI CCATC 6 cut(s) 173, 586, 614, 794, 1135, 1156
BclI TGATCA 1 cut(s) 172
BcnI CCSGG 1 cut(s) 864
BcuI ACTAGT 1 cut(s) 464
BfaI CTAG 2 cut(s) 465, 971
BfuAI ACCTGC 1 cut(s) 685
BisI GCNGC 1 cut(s) 565
BlpI GCTNAGC 1 cut(s) 837
BlsI GCNGC 1 cut(s) 566
Bme1390I CCNGG 1 cut(s) 864
Bme18I GGWCC 1 cut(s) 1184
BmgT120I GGNCC 1 cut(s) 1184
BmiI GGNNCC 1 cut(s) 1146
BmrFI CCNGG 1 cut(s) 864
BmrI ACTGGG 1 cut(s) 1183
BmsI GCATC 2 cut(s) 347, 976
BmuI ACTGGG 1 cut(s) 1183
BpiI GAAGAC 1 cut(s) 1034
BplI GAGNNNNNCTC 4 cut(s) 123, 155, 149, 181
BpmI CTGGAG 1 cut(s) 497
Bpu1102I GCTNAGC 1 cut(s) 837
Bpu14I TTCGAA 1 cut(s) 286
BpuMI CCSGG 1 cut(s) 864
BsaWI WCCGGW 1 cut(s) 406
Bse1I ACTGG 2 cut(s) 678, 1178
Bse3DI GCAATG 3 cut(s) 14, 990, 1043
BseGI GGATG 2 cut(s) 597, 1146
BseMI GCAATG 3 cut(s) 14, 990, 1043
BseMII CTCAG 3 cut(s) 294, 726, 851
BseNI ACTGG 2 cut(s) 678, 1178
Bsh1236I CGCG 1 cut(s) 317
BshFI GGCC 2 cut(s) 890, 1106
BshNI GGYRCC 1 cut(s) 1144
BsiSI CCGG 2 cut(s) 407, 864
BslFI GGGAC 4 cut(s) 119, 176, 523, 1190
BsmFI GGGAC 4 cut(s) 119, 176, 523, 1190
BsmI GAATGC 3 cut(s) 381, 571, 809
BsnI GGCC 2 cut(s) 890, 1106
Bsp119I TTCGAA 1 cut(s) 286
Bsp143I GATC 5 cut(s) 172, 403, 505, 581, 660
Bsp1720I GCTNAGC 1 cut(s) 837
BspACI CCGC 4 cut(s) 196, 565, 1094, 1187
BspANI GGCC 2 cut(s) 890, 1106
BspCNI CTCAG 3 cut(s) 295, 727, 850
BspFNI CGCG 1 cut(s) 317
BspHI TCATGA 2 cut(s) 175, 982
BspLI GGNNCC 1 cut(s) 1146
BspMI ACCTGC 1 cut(s) 685
BspPI GGATC 1 cut(s) 398
BspT104I TTCGAA 1 cut(s) 286
BspT107I GGYRCC 1 cut(s) 1144
BsrDI GCAATG 3 cut(s) 14, 990, 1043
BsrI ACTGG 2 cut(s) 678, 1178
BssMI GATC 5 cut(s) 172, 403, 505, 581, 660
Bst4CI ACNGT 4 cut(s) 550, 651, 667, 1102
BstAPI GCANNNNNTGC 1 cut(s) 1151
BstBI TTCGAA 1 cut(s) 286
BstC8I GCNNGC 3 cut(s) 569, 794, 843
BstDEI CTNAG 4 cut(s) 142, 303, 735, 837
BstF5I GGATG 2 cut(s) 597, 1146
BstFNI CGCG 1 cut(s) 317
BstHHI GCGC 1 cut(s) 317
BstKTI GATC 5 cut(s) 175, 406, 508, 584, 663
BstMBI GATC 5 cut(s) 172, 403, 505, 581, 660
BstMWI GCNNNNNNNGC 2 cut(s) 842, 1151
BstNSI RCATGY 4 cut(s) 485, 571, 845, 873
BstSCI CCNGG 1 cut(s) 862
BstUI CGCG 1 cut(s) 317
BstV2I GAAGAC 1 cut(s) 1034
BsuRI GGCC 2 cut(s) 890, 1106
BtsCI GGATG 2 cut(s) 597, 1146
BtsIMutI CAGTG 4 cut(s) 546, 656, 663, 1107
BveI ACCTGC 1 cut(s) 685
Cac8I GCNNGC 3 cut(s) 569, 794, 843
CciI TCATGA 2 cut(s) 175, 982
CfoI GCGC 1 cut(s) 317
Cfr13I GGNCC 1 cut(s) 1184
CseI GACGC 2 cut(s) 349, 796
Csp6I GTAC 2 cut(s) 1001, 1079
CspCI CAANNNNNGTGG 2 cut(s) 58, 93
CviAII CATG 7 cut(s) 176, 482, 535, 568, 842, 870, 983
CviQI GTAC 2 cut(s) 1001, 1079
DdeI CTNAG 4 cut(s) 142, 303, 735, 837
DpnI GATC 5 cut(s) 174, 405, 507, 583, 662
DpnII GATC 5 cut(s) 172, 403, 505, 581, 660
DrdI GACNNNNNNGTC 1 cut(s) 191
DseDI GACNNNNNNGTC 1 cut(s) 191
EaeI YGGCCR 1 cut(s) 1104
Eco32I GATATC 1 cut(s) 376
Eco47I GGWCC 1 cut(s) 1184
Eco57I CTGAAG 3 cut(s) 747, 1182, 1194
EcoRV GATATC 1 cut(s) 376
EcoT22I ATGCAT 1 cut(s) 573
FaeI CATG 7 cut(s) 179, 485, 538, 571, 845, 873, 986
FalI AAGNNNNNCTT 2 cut(s) 245, 277
FaqI GGGAC 4 cut(s) 119, 176, 523, 1190
FatI CATG 7 cut(s) 175, 481, 534, 567, 841, 869, 982
FbaI TGATCA 1 cut(s) 172
Fnu4HI GCNGC 1 cut(s) 565
FokI GGATG 2 cut(s) 604, 1153
Fsp4HI GCNGC 1 cut(s) 565
FspBI CTAG 2 cut(s) 465, 971
GlaI GCGC 1 cut(s) 316
GluI GCNGC 1 cut(s) 565
GsuI CTGGAG 1 cut(s) 497
HaeIII GGCC 2 cut(s) 890, 1106
HapII CCGG 2 cut(s) 407, 864
HgaI GACGC 2 cut(s) 349, 796
HhaI GCGC 1 cut(s) 317
Hin1II CATG 7 cut(s) 179, 485, 538, 571, 845, 873, 986
Hin6I GCGC 1 cut(s) 315
HinP1I GCGC 1 cut(s) 315
HinfI GANTC 9 cut(s) 60, 132, 164, 299, 472, 739, 746, 1193, 1205
HpaII CCGG 2 cut(s) 407, 864
HphI GGTGA 3 cut(s) 17, 194, 1029
Hpy166II GTNNAC 2 cut(s) 10, 46
Hpy188I TCNGA 9 cut(s) 59, 304, 471, 510, 577, 657, 766, 914, 1015
Hpy188III TCNNGA 9 cut(s) 32, 176, 476, 589, 743, 875, 983, 1024, 1072
Hpy8I GTNNAC 2 cut(s) 10, 46
Hpy99I CGWCG 1 cut(s) 661
HpyAV CCTTC 5 cut(s) 38, 527, 612, 880, 1042
HpyCH4III ACNGT 4 cut(s) 550, 651, 667, 1102
HpyCH4IV ACGT 2 cut(s) 1007, 1081
HpyCH4V TGCA 7 cut(s) 429, 571, 694, 708, 995, 1135, 1154
HpyF10VI GCNNNNNNNGC 2 cut(s) 842, 1151
HpyF3I CTNAG 4 cut(s) 142, 303, 735, 837
HpySE526I ACGT 2 cut(s) 1007, 1081
Hsp92II CATG 7 cut(s) 179, 485, 538, 571, 845, 873, 986
HspAI GCGC 1 cut(s) 315
Ksp22I TGATCA 1 cut(s) 172
Kzo9I GATC 5 cut(s) 172, 403, 505, 581, 660
LmnI GCTCC 1 cut(s) 478
LweI GCATC 2 cut(s) 347, 976
MaeI CTAG 2 cut(s) 465, 971
MaeII ACGT 2 cut(s) 1007, 1081
MaeIII GTNAC 2 cut(s) 182, 1063
MalI GATC 5 cut(s) 174, 405, 507, 583, 662
MboI GATC 5 cut(s) 172, 403, 505, 581, 660
MboII GAAGA 5 cut(s) 859, 929, 1039, 1188, 1200
MlsI TGGCCA 1 cut(s) 1106
MluCI AATT 8 cut(s) 455, 673, 686, 767, 858, 962, 1018, 1168
MluNI TGGCCA 1 cut(s) 1106
MlyI GAGTC 3 cut(s) 54, 141, 158
MmeI TCCRAC 3 cut(s) 82, 249, 680
MnlI CCTC 6 cut(s) 33, 149, 151, 444, 991, 1095
Mox20I TGGCCA 1 cut(s) 1106
Mph1103I ATGCAT 1 cut(s) 573
MroXI GAANNNNTTC 1 cut(s) 1031
MscI TGGCCA 1 cut(s) 1106
MseI TTAA 5 cut(s) 308, 623, 684, 798, 1166
MslI CAYNNNNRTG 2 cut(s) 180, 649
Msp20I TGGCCA 1 cut(s) 1106
MspI CCGG 2 cut(s) 407, 864
MspR9I CCNGG 1 cut(s) 864
Mva1269I GAATGC 3 cut(s) 381, 571, 809
MvnI CGCG 1 cut(s) 317
MwoI GCNNNNNNNGC 2 cut(s) 842, 1151
NciI CCSGG 1 cut(s) 864
NdeII GATC 5 cut(s) 172, 403, 505, 581, 660
NlaIII CATG 7 cut(s) 179, 485, 538, 571, 845, 873, 986
NlaIV GGNNCC 1 cut(s) 1146
NmuCI GTSAC 1 cut(s) 182
NsiI ATGCAT 1 cut(s) 573
NspI RCATGY 4 cut(s) 485, 571, 845, 873
NspV TTCGAA 1 cut(s) 286
PaeI GCATGC 2 cut(s) 571, 845
PagI TCATGA 2 cut(s) 175, 982
PciI ACATGT 1 cut(s) 869
PctI GAATGC 3 cut(s) 381, 571, 809
PdmI GAANNNNTTC 1 cut(s) 1031
PfeI GAWTC 6 cut(s) 299, 472, 739, 746, 1193, 1205
PfoI TCCNGGA 1 cut(s) 862
PkrI GCNGC 1 cut(s) 566
PleI GAGTC 3 cut(s) 54, 140, 158
PpsI GAGTC 3 cut(s) 54, 140, 158
PscI ACATGT 1 cut(s) 869
PspN4I GGNNCC 1 cut(s) 1146
PspPI GGNCC 1 cut(s) 1184
RsaI GTAC 2 cut(s) 1002, 1080
RsaNI GTAC 2 cut(s) 1001, 1079
RseI CAYNNNNRTG 2 cut(s) 180, 649
SaqAI TTAA 5 cut(s) 308, 623, 684, 798, 1166
SatI GCNGC 1 cut(s) 565
Sau3AI GATC 5 cut(s) 172, 403, 505, 581, 660
Sau96I GGNCC 1 cut(s) 1184
SchI GAGTC 3 cut(s) 54, 141, 158
ScrFI CCNGG 1 cut(s) 864
SfaNI GCATC 2 cut(s) 347, 976
SfuI TTCGAA 1 cut(s) 286
SinI GGWCC 1 cut(s) 1184
SmiMI CAYNNNNRTG 2 cut(s) 180, 649
SpeI ACTAGT 1 cut(s) 464
SphI GCATGC 2 cut(s) 571, 845
Sse9I AATT 8 cut(s) 455, 673, 686, 767, 858, 962, 1018, 1168
SsiI CCGC 4 cut(s) 196, 565, 1094, 1187
SspI AATATT 1 cut(s) 895
SspMI CTAG 2 cut(s) 465, 971
StyD4I CCNGG 1 cut(s) 862
TaaI ACNGT 4 cut(s) 550, 651, 667, 1102
TaiI ACGT 2 cut(s) 1010, 1084
TaqI TCGA 6 cut(s) 150, 286, 609, 874, 957, 1073
TasI AATT 8 cut(s) 455, 673, 686, 767, 858, 962, 1018, 1168
TauI GCSGC 1 cut(s) 567
TfiI GAWTC 6 cut(s) 299, 472, 739, 746, 1193, 1205
Tru1I TTAA 5 cut(s) 308, 623, 684, 798, 1166
Tru9I TTAA 5 cut(s) 308, 623, 684, 798, 1166
TscAI CASTG 4 cut(s) 553, 656, 670, 1107
TseFI GTSAC 1 cut(s) 182
Tsp45I GTSAC 1 cut(s) 182
TspDTI ATGAA 4 cut(s) 202, 251, 770, 816
TspGWI ACGGA 1 cut(s) 510
TspRI CASTG 4 cut(s) 553, 656, 670, 1107
VpaK11BI GGWCC 1 cut(s) 1184
XapI RAATTY 5 cut(s) 673, 686, 767, 858, 1018
XceI RCATGY 4 cut(s) 485, 571, 845, 873
XmnI GAANNNNTTC 1 cut(s) 1031
XspI CTAG 2 cut(s) 465, 971
Zsp2I ATGCAT 1 cut(s) 573
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.