Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr6

Orthogroup ID

OG0003877

Physical Location & Seq

Genomic Coordinates Forward (+)

19103597 .. 19106281

Gene Structure 2685 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom06g14320.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2223 bp

ATGGTGGCATTGCTAGTAGCAAAAGCTAGCTGCAGCTTATCCAAAAGGCGCCAAACAATGGCTCTCATTCGCCGCCAACTCTCTCCTCCTCCGTTACTTCCCTGCTCCCACAGCCACCCTCCACCAAACCACCACTTCCCAAAACCCTGCCTCTTCGTATTCTCCAAAAATCTCAGACTCTTCTCTCTCTACTCAGCTCCGCCCACTCCTTCCTCTCTCTCCTCCGCTCCCATTTTCCTCCCCTTTCTTCAAAACCAAGAGGAAGAAGACGAAGACGAAACTGAAGAAGAAACAGAAGAGCCACCAGCACTCGAAGAAGATGAAGAAGAGGAGGAGTACCCAGATGACCCAATTCTCAGATTCTTCAAGTCCCGCACTTCAACCCAAGACCCTGAGCGCGAAGGCAAGTTCTCTCTCCAAAAGAACCGCCGCTCCGCTTGGCGTTTGGCTGACGGTACCCACCTCGCAGACGAATCGGAACCCGAAACCGGCGTCAAAAAACTGCTCGGAGAACAGAAACAACAAGTTGGTCCAATCAAATTTGGTTCAAATGCATCGCCTGAGGGAATTGTCCAAGAGATTCTGCAAAAGGCAAGGACTTTGCCGCAGAATTTGACGTTGGGAGAGGCTTTGGGGGGTTTCGAAGGAAGGGTTGGGGAGAAAGAGTGTGTGAGAGTATTGGAGGTGATGGGTGAGGAGGGGTTATTGGTGGGTTGCTTGTATTTCTTTGAGTGGATGGGTTTGCAGGAGCCGTCTCTTGTGACGCCTCGGGCGTGTTCGGTTTTGTTTCCGATGCTGGGGAGAGCTGGTATGGGGGATAAGTTGATGATTTTGTTTAGGAATTTACCGGCCAAGAAGGAGTTCTGGGATGTTCATGTGTATAATGCTGCAATTTCGGGCTTGATGTGCTCCAGGAGGTATGATGATGCTTGGAAGGTGTTTGAGACTATGGAAGCAAATAATACCCTTCCGGATCATGTGACATGTTCAATAATGATTACTGTCATGAGAAAAGTTGGACGTAGTGCAAAAGATTCATGGCAGTTCTTCGAGAGAATGAACAGGAAAGGTGTCAAATGGAGTCAAGAAGTTTTGGGTGCTCTTATAAAATCATTCTGCGATGAGGGGTTGAAGAGTGAAGCCCTTATCATTCAAGTAGAAATGGAGAAGAAAGGAGTCTCATCAAATGCCATTGTGTATAACACTTTAATGGATGCCTTTTGTAATACAAACCAAGTTGAAGAAGCTGAAGGTCTCTTTGCCGAGATGAAATCAAAGGGGATTAAACCAACATCTGCCACTTTTAATGTTCTAATGAGTGCATACAGCAGAAAAATGGAGCCGGAGATTGTTGAGAAGCTGCTGGTCGAAATGCAGGATATGGGACTAAAGCCAAATGTCAAATCCTATACTTGTCTTATCAGTGCATATGGGAGGCAGAAGAAGATGAGTGATATGGCTGCGGATGCTTTTTTGAGGATGAAGAAGGTTGGTATTTGTCCAACATCACATTCTTATACAGCTCTTATTCACGCGTTTTCAGTTAGCGGCTGGCATGAGAAGGCTTACATTGCATTTGAAAACATGCAAAAAGAAGGACTAAAGCCCTCCATAGAAACCTATACTGCTTTACTGGATGCATTTAGGCGTGCTGGTGACGTGCAGATGTTGATGAAAATTTGGAAACTGATGATCAAAGAGAAGATTGTGGGGACTAAGGTGACATACAACATTCTTCTCGATGGATTTGCCAAACAAGGGCACTATGTGGAAGCAAGAGATGTGATTTCCGAATTTGGTAATGTAGGCTTGCAGCCAACAGTGATGACATATAATATGCTGATGAATGCGTATGCACGGGGAGGGCAACACTCAAAGTTGCCACAGCTGTTGAAAGAGATGGCAGCTCTCAAGTTAAAACCTGATTCAGTAACTTACTCGACCATGATTTATGCCTATGTTCGTGTTCGTGATTTCAGACGAGCATTTTTCTACCACAAGCAAATGGTAAAAAATGGACAAGTGCCAGATGCCAGGTCTTATGAGAAGCTTAGGTCAATTTTGGATGTGAAAGCTGCACGGAAGAACAAAAAGGATAAGAGTGCTATACTCGGTATAATTAATAGCAAAATGGGTTTGTTGAAAGTTAAAAAGAAGGGAAAGAAAGATGAGTTCTGGAAGAACAAGAACAAGCGTTATGTCAGAACCGATAACTCAGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

741

Amino Acids

83.93

Weight (kDa)

8.95

Isoelectric Point (pI)

46.94

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	290 - 335	2.4e-10	PPR repeat family
PPR_long	PF17177	302 - 439	3.2e-07	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	387 - 442	2.3e-12	Pentatricopeptide repeat domain
TPR_24	PF23276	388 - 482	1e-06	Fungal tetratrico peptide repeats
PPR_1	PF12854	391 - 424	1.1e-07	PPR repeat
PPR_2	PF13041	396 - 443	2.9e-16	PPR repeat family
PPR	PF01535	399 - 428	2.1e-08	PPR repeat
PPR_long	PF17177	419 - 523	1.7e-08	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	432 - 477	8.8e-10	PPR repeat family
PPR_3	PF13812	453 - 513	1.1e-10	Pentatricopeptide repeat domain
PPR_2	PF13041	465 - 513	6.8e-10	PPR repeat family
PPR_long	PF17177	485 - 587	5.8e-07	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	505 - 548	1.3e-08	PPR repeat family
PPR_3	PF13812	526 - 581	1.1e-11	Pentatricopeptide repeat domain
PPR_2	PF13041	536 - 585	2e-09	PPR repeat family
PPR_2	PF13041	574 - 620	3.4e-10	PPR repeat family
PPR_3	PF13812	574 - 618	4.6e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	606 - 654	2.5e-11	PPR repeat family
PPR_2	PF13041	641 - 681	7.5e-07	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0003877)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G50280
fragaria_vesca	FvH4_5g07010 FvH4_5g07010
malus_domestica	MD06G1160200.v1.1
prunus_persica	Prupe.5G160200_v2.0.a1
pyrus_communis	pycom06g14320
rosa_chinensis	RchiOBHm_Chr2g0103631 RchiOBHm_Chr7g0191671
rosa_laevigata	RLG00000004435 RLG00000016595 RLG00000030786
rosa_multiflora	Rmu_co8329853.1_g000001 Rmu_co8381307.1_g000001 Rmu_sc0001123.1_g000010 Rmu_sc0001530.1_g000019 Rmu_sc0001852.1_g000012 Rmu_sc0018746.1_g000006
rosa_roxburghii	Rroxscaffold_157G00438490 Rroxscaffold_3G00263660
rosa_rugosa	Rorug02G0116400 Rorug06G0510800
rosa_samantha	Rh2AG102000 Rh2BG023000 Rh2DG103300 Rh2DG170000 Rh3CG338800 Rh6BG097500 Rh7AG119800 Rh7AG120000 Rh7BG122100 Rh7BG214500 Rh7CG124700 Rh7DG122900
rosa_wichuraiana	Rw7G010220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1106
Acc65I	GGTACC	1 cut(s)	455
AccB1I	GGYRCC	2 cut(s)	48, 455
AccB7I	CCANNNNNTGG	1 cut(s)	58
AccBSI	CCGCTC	2 cut(s)	227, 432
AccII	CGCG	2 cut(s)	399, 1535
AccIII	TCCGGA	1 cut(s)	970
AclWI	GGATC	1 cut(s)	981
AcoI	YGGCCR	1 cut(s)	849
AcsI	RAATTY	5 cut(s)	539, 610, 841, 1677, 1793
AcuI	CTGAAG	2 cut(s)	303, 1269
AcyI	GRCGYC	3 cut(s)	49, 492, 764
AdeI	CACNNNGTG	1 cut(s)	1768
AfaI	GTAC	2 cut(s)	338, 457
AfiI	CCNNNNNNNGG	4 cut(s)	58, 488, 797, 1758
AflIII	ACRYGT	2 cut(s)	983, 1533
AjiI	CACGTC	1 cut(s)	1660
AjnI	CCWGG	2 cut(s)	911, 2033
AjuI	GAANNNNNNNTTGG	8 cut(s)	602, 634, 636, 668, 845, 877, 1495, 1527
AloI	GAACNNNNNNTCC	2 cut(s)	416, 448
Alw21I	GWGCWC	2 cut(s)	911, 1102
Alw26I	GTCTC	4 cut(s)	759, 938, 1183, 1259
AlwI	GGATC	1 cut(s)	981
Ama87I	CYCGRG	1 cut(s)	768
Aor13HI	TCCGGA	1 cut(s)	970
AoxI	GGCC	1 cut(s)	849
ApeKI	GCWGC	8 cut(s)	30, 33, 887, 1360, 1460, 1813, 1904, 2075
ApoI	RAATTY	5 cut(s)	539, 610, 841, 1677, 1793
ArsI	GACNNNNNNTTYG	2 cut(s)	477, 509
AseI	ATTAAT	1 cut(s)	2121
Asp700I	GAANNNNTTC	1 cut(s)	860
Asp718I	GGTACC	1 cut(s)	455
AspLEI	GCGC	2 cut(s)	51, 399
AspS9I	GGNCC	1 cut(s)	530
AsuHPI	GGTGA	4 cut(s)	697, 704, 1667, 1732
AsuII	TTCGAA	1 cut(s)	642
AsuNHI	GCTAGC	1 cut(s)	26
AvaI	CYCGRG	1 cut(s)	768
AvaII	GGWCC	1 cut(s)	530
AxyI	CCTNAGG	1 cut(s)	561
BaeGI	GKGCMC	1 cut(s)	1764
BanI	GGYRCC	2 cut(s)	48, 455
BbsI	GAAGAC	2 cut(s)	273, 279
Bbv12I	GWGCWC	2 cut(s)	911, 1102
BbvI	GCAGC	8 cut(s)	17, 45, 874, 1347, 1447, 1825, 1916, 2062
BccI	CCATC	4 cut(s)	682, 730, 1736, 1894
BceAI	ACGGC	1 cut(s)	736
BcgI	CGANNNNNNTGC	4 cut(s)	456, 490, 1730, 1764
BciT130I	CCWGG	2 cut(s)	913, 2035
BclI	TGATCA	1 cut(s)	1692
BcoDI	GTCTC	4 cut(s)	759, 938, 1183, 1259
BfaI	CTAG	2 cut(s)	14, 27
BfmI	CTRYAG	1 cut(s)	31
BfoI	RGCGCY	1 cut(s)	52
Bme1390I	CCNGG	2 cut(s)	913, 2035
Bme18I	GGWCC	1 cut(s)	530
BmeT110I	CYCGRG	1 cut(s)	768
BmgBI	CACGTC	1 cut(s)	1660
BmgT120I	GGNCC	1 cut(s)	530
BmiI	GGNNCC	5 cut(s)	50, 457, 480, 750, 1341
BmrFI	CCNGG	2 cut(s)	913, 2035
BmsI	GCATC	7 cut(s)	563, 783, 916, 1204, 1456, 1627, 2020
BmtI	GCTAGC	1 cut(s)	30
BpiI	GAAGAC	2 cut(s)	273, 279
BpmI	CTGGAG	1 cut(s)	895
Bpu10I	CCTNAGC	2 cut(s)	393, 2051
Bpu14I	TTCGAA	1 cut(s)	642
BpuEI	CTTGAG	1 cut(s)	1895
BsaHI	GRCGYC	3 cut(s)	49, 492, 764
BsaI	GGTCTC	1 cut(s)	1259
BsaJI	CCNNGG	1 cut(s)	767
BsaWI	WCCGGW	1 cut(s)	970
BsaXI	ACNNNNNCTCC	6 cut(s)	416, 446, 650, 680, 793, 823
Bsc4I	CCNNNNNNNGG	4 cut(s)	58, 488, 797, 1758
Bse118I	RCCGGY	2 cut(s)	488, 847
Bse1I	ACTGG	1 cut(s)	1638
Bse21I	CCTNAGG	1 cut(s)	561
Bse3DI	GCAATG	2 cut(s)	8, 1569
BseAI	TCCGGA	1 cut(s)	970
BseBI	CCWGG	2 cut(s)	913, 2035
BseDI	CCNNGG	1 cut(s)	767
BseGI	GGATG	7 cut(s)	741, 874, 1219, 1471, 1485, 1642, 2071
BseLI	CCNNNNNNNGG	4 cut(s)	58, 488, 797, 1758
BseMI	GCAATG	2 cut(s)	8, 1569
BseMII	CTCAG	5 cut(s)	187, 207, 370, 384, 552
BseNI	ACTGG	1 cut(s)	1638
BseRI	GAGGAG	6 cut(s)	75, 78, 211, 344, 347, 710
BseSI	GKGCMC	1 cut(s)	1764
BseXI	GCAGC	8 cut(s)	17, 45, 874, 1347, 1447, 1825, 1916, 2062
BseYI	CCCAGC	1 cut(s)	796
BsgI	GTGCAG	2 cut(s)	1682, 2061
Bsh1236I	CGCG	2 cut(s)	399, 1535
BshFI	GGCC	1 cut(s)	851
BshNI	GGYRCC	2 cut(s)	48, 455
BsiHKAI	GWGCWC	2 cut(s)	911, 1102
BsiHKCI	CYCGRG	1 cut(s)	768
BsiSI	CCGG	4 cut(s)	489, 848, 971, 1343
BslFI	GGGAC	3 cut(s)	355, 1398, 1726
BslI	CCNNNNNNNGG	4 cut(s)	58, 488, 797, 1758
BsmAI	GTCTC	4 cut(s)	759, 938, 1183, 1259
BsmBI	CGTCTC	1 cut(s)	759
BsmFI	GGGAC	3 cut(s)	355, 1398, 1726
BsmI	GAATGC	1 cut(s)	1852
BsnI	GGCC	1 cut(s)	851
Bso31I	GGTCTC	1 cut(s)	1259
BsoBI	CYCGRG	1 cut(s)	768
Bsp119I	TTCGAA	1 cut(s)	642
Bsp1286I	GDGCHC	3 cut(s)	911, 1102, 1764
Bsp13I	TCCGGA	1 cut(s)	970
Bsp143I	GATC	2 cut(s)	973, 1692
BspANI	GGCC	1 cut(s)	851
BspCNI	CTCAG	5 cut(s)	186, 206, 369, 385, 553
BspEI	TCCGGA	1 cut(s)	970
BspFNI	CGCG	2 cut(s)	399, 1535
BspHI	TCATGA	1 cut(s)	1005
BspLI	GGNNCC	5 cut(s)	50, 457, 480, 750, 1341
BspMAI	CTGCAG	1 cut(s)	35
BspOI	GCTAGC	1 cut(s)	30
BspPI	GGATC	1 cut(s)	981
BspQI	GCTCTTC	1 cut(s)	291
BspT104I	TTCGAA	1 cut(s)	642
BspT107I	GGYRCC	2 cut(s)	48, 455
BspTNI	GGTCTC	1 cut(s)	1259
BsrBI	CCGCTC	2 cut(s)	227, 432
BsrDI	GCAATG	2 cut(s)	8, 1569
BsrFI	RCCGGY	2 cut(s)	488, 847
BsrI	ACTGG	1 cut(s)	1638
BssAI	RCCGGY	2 cut(s)	488, 847
BssECI	CCNNGG	1 cut(s)	767
BssMI	GATC	2 cut(s)	973, 1692
BssNI	GRCGYC	3 cut(s)	49, 492, 764
Bst2UI	CCWGG	2 cut(s)	913, 2035
Bst4CI	ACNGT	3 cut(s)	455, 1003, 1822
Bst6I	CTCTTC	5 cut(s)	158, 185, 291, 321, 1127
BstACI	GRCGYC	3 cut(s)	49, 492, 764
BstBI	TTCGAA	1 cut(s)	642
BstC8I	GCNNGC	4 cut(s)	28, 1553, 1650, 1811
BstDEI	CTNAG	8 cut(s)	173, 193, 356, 393, 561, 1716, 2051, 2215
BstF5I	GGATG	7 cut(s)	741, 874, 1219, 1471, 1485, 1642, 2071
BstFNI	CGCG	2 cut(s)	399, 1535
BstH2I	RGCGCY	1 cut(s)	52
BstHHI	GCGC	2 cut(s)	51, 399
BstKTI	GATC	2 cut(s)	976, 1695
BstMAI	GTCTC	4 cut(s)	759, 938, 1183, 1259
BstMBI	GATC	2 cut(s)	973, 1692
BstMWI	GCNNNNNNNGC	4 cut(s)	111, 906, 1466, 1571
BstNI	CCWGG	2 cut(s)	913, 2035
BstNSI	RCATGY	2 cut(s)	987, 1588
BstSCI	CCNGG	2 cut(s)	911, 2033
BstSFI	CTRYAG	1 cut(s)	31
BstSLI	GKGCMC	1 cut(s)	1764
BstUI	CGCG	2 cut(s)	399, 1535
BstV1I	GCAGC	8 cut(s)	17, 45, 874, 1347, 1447, 1825, 1916, 2062
BstV2I	GAAGAC	2 cut(s)	273, 279
Bsu36I	CCTNAGG	1 cut(s)	561
BsuRI	GGCC	1 cut(s)	851
BtgZI	GCGATG	2 cut(s)	540, 1134
BtrI	CACGTC	1 cut(s)	1660
BtsCI	GGATG	7 cut(s)	741, 874, 1219, 1471, 1485, 1642, 2071
BtsIMutI	CAGTG	2 cut(s)	1429, 1827
Cac8I	GCNNGC	4 cut(s)	28, 1553, 1650, 1811
CciI	TCATGA	1 cut(s)	1005
CfoI	GCGC	2 cut(s)	51, 399
Cfr10I	RCCGGY	2 cut(s)	488, 847
Cfr13I	GGNCC	1 cut(s)	530
CseI	GACGC	2 cut(s)	481, 772
Csp6I	GTAC	2 cut(s)	337, 456
CspCI	CAANNNNNGTGG	2 cut(s)	1872, 1907
CviAII	CATG	8 cut(s)	875, 977, 984, 1006, 1038, 1556, 1585, 1945
CviQI	GTAC	2 cut(s)	337, 456
DdeI	CTNAG	8 cut(s)	173, 193, 356, 393, 561, 1716, 2051, 2215
DinI	GGCGCC	1 cut(s)	50
DpnI	GATC	2 cut(s)	975, 1694
DpnII	GATC	2 cut(s)	973, 1692
DraIII	CACNNNGTG	1 cut(s)	1768
EaeI	YGGCCR	1 cut(s)	849
Eam1104I	CTCTTC	5 cut(s)	158, 185, 291, 321, 1127
EarI	CTCTTC	5 cut(s)	158, 185, 291, 321, 1127
EciI	GGCGGA	1 cut(s)	189
Eco31I	GGTCTC	1 cut(s)	1259
Eco47I	GGWCC	1 cut(s)	530
Eco57I	CTGAAG	2 cut(s)	303, 1269
Eco81I	CCTNAGG	1 cut(s)	561
Eco88I	CYCGRG	1 cut(s)	768
EcoRII	CCWGG	2 cut(s)	911, 2033
EcoT22I	ATGCAT	2 cut(s)	556, 1642
EgeI	GGCGCC	1 cut(s)	50
EheI	GGCGCC	1 cut(s)	50
Esp3I	CGTCTC	1 cut(s)	759
FaeI	CATG	8 cut(s)	878, 980, 987, 1009, 1041, 1559, 1588, 1948
FaqI	GGGAC	3 cut(s)	355, 1398, 1726
FatI	CATG	8 cut(s)	874, 976, 983, 1005, 1037, 1555, 1584, 1944
FauI	CCCGC	1 cut(s)	380
FauNDI	CATATG	1 cut(s)	1429
FbaI	TGATCA	1 cut(s)	1692
FokI	GGATG	7 cut(s)	748, 881, 1226, 1478, 1492, 1649, 2078
FspBI	CTAG	2 cut(s)	14, 27
GlaI	GCGC	2 cut(s)	50, 398
GsaI	CCCAGC	1 cut(s)	800
GsuI	CTGGAG	1 cut(s)	895
HaeII	RGCGCY	1 cut(s)	52
HaeIII	GGCC	1 cut(s)	851
HapII	CCGG	4 cut(s)	489, 848, 971, 1343
HgaI	GACGC	2 cut(s)	481, 772
HhaI	GCGC	2 cut(s)	51, 399
Hin1I	GRCGYC	3 cut(s)	49, 492, 764
Hin1II	CATG	8 cut(s)	878, 980, 987, 1009, 1041, 1559, 1588, 1948
Hin6I	GCGC	2 cut(s)	49, 397
HinP1I	GCGC	2 cut(s)	49, 397
HindIII	AAGCTT	1 cut(s)	2048
HinfI	GANTC	8 cut(s)	177, 360, 473, 580, 1034, 1081, 1176, 1925
HpaII	CCGG	4 cut(s)	489, 848, 971, 1343
HphI	GGTGA	4 cut(s)	697, 704, 1667, 1732
Hpy188I	TCNGA	9 cut(s)	176, 359, 478, 509, 792, 1792, 1979, 2204, 2218
Hpy188III	TCNNGA	7 cut(s)	971, 1006, 1051, 1085, 1739, 1970, 2176
HpyCH4III	ACNGT	3 cut(s)	455, 1003, 1822
HpyCH4IV	ACGT	3 cut(s)	617, 1021, 1659
HpyF10VI	GCNNNNNNNGC	4 cut(s)	111, 906, 1466, 1571
HpyF3I	CTNAG	8 cut(s)	173, 193, 356, 393, 561, 1716, 2051, 2215
HpySE526I	ACGT	3 cut(s)	617, 1021, 1659
Hsp92I	GRCGYC	3 cut(s)	49, 492, 764
Hsp92II	CATG	8 cut(s)	878, 980, 987, 1009, 1041, 1559, 1588, 1948
HspAI	GCGC	2 cut(s)	49, 397
KasI	GGCGCC	1 cut(s)	48
Kpn2I	TCCGGA	1 cut(s)	970
KpnI	GGTACC	1 cut(s)	459
Ksp22I	TGATCA	1 cut(s)	1692
Kzo9I	GATC	2 cut(s)	973, 1692
LguI	GCTCTTC	1 cut(s)	291
LmnI	GCTCC	7 cut(s)	110, 202, 232, 437, 748, 914, 1339
Lsp1109I	GCAGC	8 cut(s)	17, 45, 874, 1347, 1447, 1825, 1916, 2062
LweI	GCATC	7 cut(s)	563, 783, 916, 1204, 1456, 1627, 2020
MaeI	CTAG	2 cut(s)	14, 27
MaeII	ACGT	3 cut(s)	617, 1021, 1659
MaeIII	GTNAC	6 cut(s)	93, 760, 979, 1655, 1720, 1930
MalI	GATC	2 cut(s)	975, 1694
MbiI	CCGCTC	2 cut(s)	227, 432
MboI	GATC	2 cut(s)	973, 1692
MhlI	GDGCHC	3 cut(s)	911, 1102, 1764
MluI	ACGCGT	1 cut(s)	1533
Mly113I	GGCGCC	1 cut(s)	49
MlyI	GAGTC	3 cut(s)	171, 1090, 1185
MmeI	TCCRAC	2 cut(s)	997, 1526
Mph1103I	ATGCAT	2 cut(s)	556, 1642
MroI	TCCGGA	1 cut(s)	970
MroXI	GAANNNNTTC	1 cut(s)	860
MseI	TTAA	6 cut(s)	1208, 1284, 1305, 1916, 2121, 2148
MslI	CAYNNNNRTG	1 cut(s)	1208
MspA1I	CMGCKG	1 cut(s)	1888
MspI	CCGG	4 cut(s)	489, 848, 971, 1343
MspR9I	CCNGG	2 cut(s)	913, 2035
Mva1269I	GAATGC	1 cut(s)	1852
MvaI	CCWGG	2 cut(s)	913, 2035
MvnI	CGCG	2 cut(s)	399, 1535
MwoI	GCNNNNNNNGC	4 cut(s)	111, 906, 1466, 1571
NarI	GGCGCC	1 cut(s)	49
NdeI	CATATG	1 cut(s)	1429
NdeII	GATC	2 cut(s)	973, 1692
NheI	GCTAGC	1 cut(s)	26
NlaIII	CATG	8 cut(s)	878, 980, 987, 1009, 1041, 1559, 1588, 1948
NlaIV	GGNNCC	5 cut(s)	50, 457, 480, 750, 1341
NmeAIII	GCCGAG	1 cut(s)	1288
NmuCI	GTSAC	4 cut(s)	760, 979, 1655, 1720
NsiI	ATGCAT	2 cut(s)	556, 1642
NspI	RCATGY	2 cut(s)	987, 1588
NspV	TTCGAA	1 cut(s)	642
PagI	TCATGA	1 cut(s)	1005
PciI	ACATGT	1 cut(s)	983
PciSI	GCTCTTC	1 cut(s)	291
PcsI	WCGNNNNNNNCGW	1 cut(s)	770
PctI	GAATGC	1 cut(s)	1852
PdmI	GAANNNNTTC	1 cut(s)	860
PfeI	GAWTC	5 cut(s)	360, 473, 580, 1034, 1925
PflMI	CCANNNNNTGG	1 cut(s)	58
PfoI	TCCNGGA	1 cut(s)	911
PleI	GAGTC	3 cut(s)	171, 1089, 1184
PluTI	GGCGCC	1 cut(s)	52
PpsI	GAGTC	3 cut(s)	171, 1089, 1184
PscI	ACATGT	1 cut(s)	983
PshBI	ATTAAT	1 cut(s)	2121
PsiI	TTATAA	1 cut(s)	1106
Psp6I	CCWGG	2 cut(s)	911, 2033
PspFI	CCCAGC	1 cut(s)	796
PspGI	CCWGG	2 cut(s)	911, 2033
PspN4I	GGNNCC	5 cut(s)	50, 457, 480, 750, 1341
PspPI	GGNCC	1 cut(s)	530
PstI	CTGCAG	1 cut(s)	35
PvuII	CAGCTG	1 cut(s)	1888
RsaI	GTAC	2 cut(s)	338, 457
RsaNI	GTAC	2 cut(s)	337, 456
RseI	CAYNNNNRTG	1 cut(s)	1208
SapI	GCTCTTC	1 cut(s)	291
SaqAI	TTAA	6 cut(s)	1208, 1284, 1305, 1916, 2121, 2148
Sau3AI	GATC	2 cut(s)	973, 1692
Sau96I	GGNCC	1 cut(s)	530
SchI	GAGTC	3 cut(s)	171, 1090, 1185
ScrFI	CCNGG	2 cut(s)	913, 2035
SduI	GDGCHC	3 cut(s)	911, 1102, 1764
SfaNI	GCATC	7 cut(s)	563, 783, 916, 1204, 1456, 1627, 2020
SfcI	CTRYAG	1 cut(s)	31
SfoI	GGCGCC	1 cut(s)	50
SfuI	TTCGAA	1 cut(s)	642
SinI	GGWCC	1 cut(s)	530
SmiMI	CAYNNNNRTG	1 cut(s)	1208
SmlI	CTYRAG	1 cut(s)	1910
SmoI	CTYRAG	1 cut(s)	1910
SspDI	GGCGCC	1 cut(s)	48
SspMI	CTAG	2 cut(s)	14, 27
StyD4I	CCNGG	2 cut(s)	911, 2033
TaaI	ACNGT	3 cut(s)	455, 1003, 1822
TaiI	ACGT	3 cut(s)	620, 1024, 1662
TaqI	TCGA	6 cut(s)	312, 642, 1050, 1368, 1740, 1940
TauI	GCSGC	4 cut(s)	75, 432, 607, 1551
TfiI	GAWTC	5 cut(s)	360, 473, 580, 1034, 1925
Tru1I	TTAA	6 cut(s)	1208, 1284, 1305, 1916, 2121, 2148
Tru9I	TTAA	6 cut(s)	1208, 1284, 1305, 1916, 2121, 2148
TscAI	CASTG	2 cut(s)	1429, 1827
TseFI	GTSAC	4 cut(s)	760, 979, 1655, 1720
TseI	GCWGC	8 cut(s)	30, 33, 887, 1360, 1460, 1813, 1904, 2075
Tsp45I	GTSAC	4 cut(s)	760, 979, 1655, 1720
TspDTI	ATGAA	8 cut(s)	336, 863, 1026, 1073, 1283, 1496, 1688, 1859
TspGWI	ACGGA	2 cut(s)	81, 2095
TspRI	CASTG	2 cut(s)	1429, 1827
Van91I	CCANNNNNTGG	1 cut(s)	58
VpaK11BI	GGWCC	1 cut(s)	530
VspI	ATTAAT	1 cut(s)	2121
XapI	RAATTY	5 cut(s)	539, 610, 841, 1677, 1793
XceI	RCATGY	2 cut(s)	987, 1588
XmnI	GAANNNNTTC	1 cut(s)	860
XspI	CTAG	2 cut(s)	14, 27
Zsp2I	ATGCAT	2 cut(s)	556, 1642

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.