RLG00000016595

(R)-mandelonitrile lyase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0003877
Physical Location & Seq
Forward (+)
8085089 .. 8087384
2296 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016595

Sequence Viewer

Length: 681 bp
ATGGTGGATCTTGAGGCAAAGCAACAGGTGCGGCTGGGTGATTGCGCCGGCAGATTTTCTGCCTGCATACAACTTGTAGACTGCCAATGTACTGGCTTCTCCATCAGCAGTGTACTGGTCCAGGTGTCATTTGTCGTCTTGTTGGGATTAGGTGAACATGGGTATAGTGAGGAGAAGAATGAGCTCTTCTTTTTCAGTAATACAACTAACAGAGTGCCAAGCAAATGGAAACTTGATACCCACAAAGCTGATACTCCAATTAGAGACAAGAATGACACACAGATTGGAACAAAGAGAAGGCTGAGTTATGTACCTAATCCTCAACAAGACGACAAATGGCCGTCGTCTTGGAACCCACGCCGGCGTCGTCAAGAGCCAAGTTACCTGAAGTTCTTTTCCAACGCCACTGACTTCCCACCGGAGGTCTACTATAATGTCTACATTATCAGCGGAGGTGGCACTGCTGGGTGCCCTTTGGCTGCGACACTATCATCCAGGTTTCGGGTCCTCCTGCTCAAAAGAGGTGGCGTTGCTTATGGCAATCAAACCTTGATGACCCAGGATGGTTTCTGTCAGCGCTCATGGATGTCAATGCCTTTGACTCTCCGACACAAGCCTTCACATCTGAAGATGGTGTTCCAAATGTCCGAGGACGCATTCTTCGAGGCAGCAGGGCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00460 ko01110 map00460 map01110
Pfam Domains
Protein Families
GMC_oxred_C GMC_oxred_N

Protein Analysis

227

Amino Acids

25.55

Weight (kDa)

9.04

Isoelectric Point (pI)

51.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 468
AccI GTMKAC 3 cut(s) 78, 426, 438
AciI CCGC 2 cut(s) 31, 450
AclWI GGATC 1 cut(s) 15
AcoI YGGCCR 1 cut(s) 338
AcuI CTGAAG 2 cut(s) 407, 647
AcyI GRCGYC 1 cut(s) 364
AfaI GTAC 3 cut(s) 91, 114, 312
AfeI AGCGCT 1 cut(s) 578
AfiI CCNNNNNNNGG 2 cut(s) 421, 501
AjnI CCWGG 3 cut(s) 120, 494, 558
AluBI AGCT 2 cut(s) 184, 248
AluI AGCT 2 cut(s) 184, 248
Alw21I GWGCWC 1 cut(s) 186
Alw26I GTCTC 1 cut(s) 258
AlwI GGATC 1 cut(s) 15
Aor51HI AGCGCT 1 cut(s) 578
AoxI GGCC 2 cut(s) 338, 674
ApeKI GCWGC 2 cut(s) 479, 668
ArsI GACNNNNNNTTYG 2 cut(s) 644, 676
AspLEI GCGC 2 cut(s) 47, 579
AspS9I GGNCC 3 cut(s) 118, 505, 674
AsuHPI GGTGA 2 cut(s) 50, 164
AvaII GGWCC 2 cut(s) 118, 505
BaeGI GKGCMC 1 cut(s) 473
BanI GGYRCC 1 cut(s) 468
BanII GRGCYC 1 cut(s) 186
Bbv12I GWGCWC 1 cut(s) 186
BbvI GCAGC 1 cut(s) 466
BccI CCATC 3 cut(s) 110, 557, 625
BceAI ACGGC 1 cut(s) 325
BciT130I CCWGG 3 cut(s) 122, 496, 560
BcoDI GTCTC 1 cut(s) 258
BfoI RGCGCY 1 cut(s) 580
BisI GCNGC 3 cut(s) 32, 480, 669
BlsI GCNGC 3 cut(s) 33, 481, 670
Bme1390I CCNGG 3 cut(s) 122, 496, 560
Bme18I GGWCC 2 cut(s) 118, 505
BmgT120I GGNCC 3 cut(s) 118, 505, 674
BmiI GGNNCC 3 cut(s) 353, 470, 506
BmrFI CCNGG 3 cut(s) 122, 496, 560
BpuEI CTTGAG 1 cut(s) 32
BsaHI GRCGYC 1 cut(s) 364
BsaJI CCNNGG 2 cut(s) 558, 648
BsaWI WCCGGW 1 cut(s) 418
Bsc4I CCNNNNNNNGG 2 cut(s) 421, 501
Bse118I RCCGGY 2 cut(s) 47, 360
Bse1I ACTGG 2 cut(s) 97, 120
BseBI CCWGG 3 cut(s) 122, 496, 560
BseDI CCNNGG 2 cut(s) 558, 648
BseGI GGATG 3 cut(s) 491, 568, 591
BseLI CCNNNNNNNGG 2 cut(s) 421, 501
BseMII CTCAG 1 cut(s) 293
BseNI ACTGG 2 cut(s) 97, 120
BseRI GAGGAG 1 cut(s) 185
BseSI GKGCMC 1 cut(s) 473
BseXI GCAGC 1 cut(s) 466
BseYI CCCAGC 2 cut(s) 34, 464
BshFI GGCC 2 cut(s) 340, 676
BshNI GGYRCC 1 cut(s) 468
BsiHKAI GWGCWC 1 cut(s) 186
BsiSI CCGG 3 cut(s) 48, 361, 419
BslI CCNNNNNNNGG 2 cut(s) 421, 501
BsmAI GTCTC 1 cut(s) 258
BsmI GAATGC 1 cut(s) 656
BsnI GGCC 2 cut(s) 340, 676
Bsp1286I GDGCHC 2 cut(s) 186, 473
Bsp143I GATC 1 cut(s) 7
BspACI CCGC 2 cut(s) 31, 450
BspANI GGCC 2 cut(s) 340, 676
BspCNI CTCAG 1 cut(s) 294
BspLI GGNNCC 3 cut(s) 353, 470, 506
BspPI GGATC 1 cut(s) 15
BspQI GCTCTTC 1 cut(s) 191
BspT107I GGYRCC 1 cut(s) 468
BsrFI RCCGGY 2 cut(s) 47, 360
BsrI ACTGG 2 cut(s) 97, 120
BssAI RCCGGY 2 cut(s) 47, 360
BssECI CCNNGG 2 cut(s) 558, 648
BssMI GATC 1 cut(s) 7
BssNI GRCGYC 1 cut(s) 364
Bst2UI CCWGG 3 cut(s) 122, 496, 560
Bst6I CTCTTC 1 cut(s) 191
BstACI GRCGYC 1 cut(s) 364
BstAPI GCANNNNNTGC 1 cut(s) 28
BstC8I GCNNGC 3 cut(s) 49, 64, 362
BstDEI CTNAG 1 cut(s) 302
BstF5I GGATG 3 cut(s) 491, 568, 591
BstH2I RGCGCY 1 cut(s) 580
BstHHI GCGC 2 cut(s) 47, 579
BstKTI GATC 1 cut(s) 10
BstMAI GTCTC 1 cut(s) 258
BstMBI GATC 1 cut(s) 7
BstMWI GCNNNNNNNGC 2 cut(s) 28, 456
BstNI CCWGG 3 cut(s) 122, 496, 560
BstSCI CCNGG 3 cut(s) 120, 494, 558
BstSLI GKGCMC 1 cut(s) 473
BstV1I GCAGC 1 cut(s) 466
BstX2I RGATCY 1 cut(s) 7
BstXI CCANNNNNNTGG 2 cut(s) 92, 225
BstYI RGATCY 1 cut(s) 7
BsuRI GGCC 2 cut(s) 340, 676
BtsCI GGATG 3 cut(s) 491, 568, 591
BtsI GCAGTG 2 cut(s) 115, 459
BtsIMutI CAGTG 3 cut(s) 115, 405, 459
Cac8I GCNNGC 3 cut(s) 49, 64, 362
CfoI GCGC 2 cut(s) 47, 579
Cfr10I RCCGGY 2 cut(s) 47, 360
Cfr13I GGNCC 3 cut(s) 118, 505, 674
CseI GACGC 2 cut(s) 353, 662
Csp6I GTAC 3 cut(s) 90, 113, 311
CspCI CAANNNNNGTGG 2 cut(s) 505, 540
CviAII CATG 3 cut(s) 158, 582, 678
CviQI GTAC 3 cut(s) 90, 113, 311
DdeI CTNAG 1 cut(s) 302
DpnI GATC 1 cut(s) 9
DpnII GATC 1 cut(s) 7
EaeI YGGCCR 1 cut(s) 338
Eam1104I CTCTTC 1 cut(s) 191
EarI CTCTTC 1 cut(s) 191
Ecl136II GAGCTC 1 cut(s) 184
Eco24I GRGCYC 1 cut(s) 186
Eco47I GGWCC 2 cut(s) 118, 505
Eco47III AGCGCT 1 cut(s) 578
Eco53kI GAGCTC 1 cut(s) 184
Eco57I CTGAAG 2 cut(s) 407, 647
EcoICRI GAGCTC 1 cut(s) 184
EcoO109I RGGNCCY 1 cut(s) 505
EcoRII CCWGG 3 cut(s) 120, 494, 558
EcoT38I GRGCYC 1 cut(s) 186
FaeI CATG 3 cut(s) 161, 585, 681
FaiI YATR 8 cut(s) 68, 159, 165, 309, 432, 537, 583, 679
FatI CATG 3 cut(s) 157, 581, 677
FblI GTMKAC 3 cut(s) 78, 426, 438
Fnu4HI GCNGC 3 cut(s) 32, 480, 669
FokI GGATG 3 cut(s) 478, 575, 598
FriOI GRGCYC 1 cut(s) 186
Fsp4HI GCNGC 3 cut(s) 32, 480, 669
GlaI GCGC 2 cut(s) 46, 578
GluI GCNGC 3 cut(s) 32, 480, 669
GsaI CCCAGC 2 cut(s) 38, 468
HaeII RGCGCY 1 cut(s) 580
HaeIII GGCC 2 cut(s) 340, 676
HapII CCGG 3 cut(s) 48, 361, 419
HgaI GACGC 2 cut(s) 353, 662
HhaI GCGC 2 cut(s) 47, 579
Hin1I GRCGYC 1 cut(s) 364
Hin1II CATG 3 cut(s) 161, 585, 681
Hin6I GCGC 2 cut(s) 45, 577
HinP1I GCGC 2 cut(s) 45, 577
HinfI GANTC 1 cut(s) 601
HpaII CCGG 3 cut(s) 48, 361, 419
HphI GGTGA 2 cut(s) 50, 164
Hpy166II GTNNAC 5 cut(s) 79, 113, 155, 427, 439
Hpy188I TCNGA 3 cut(s) 608, 627, 649
Hpy188III TCNNGA 2 cut(s) 11, 371
Hpy8I GTNNAC 5 cut(s) 79, 113, 155, 427, 439
Hpy99I CGWCG 2 cut(s) 346, 369
HpyAV CCTTC 2 cut(s) 291, 627
HpyCH4V TGCA 1 cut(s) 66
HpyF10VI GCNNNNNNNGC 2 cut(s) 28, 456
HpyF3I CTNAG 1 cut(s) 302
Hsp92I GRCGYC 1 cut(s) 364
Hsp92II CATG 3 cut(s) 161, 585, 681
HspAI GCGC 2 cut(s) 45, 577
KroI GCCGGC 2 cut(s) 47, 360
KroNI GCCGGC 2 cut(s) 49, 362
Kzo9I GATC 1 cut(s) 7
LguI GCTCTTC 1 cut(s) 191
Lsp1109I GCAGC 1 cut(s) 466
MaeIII GTNAC 1 cut(s) 380
MalI GATC 1 cut(s) 9
MboI GATC 1 cut(s) 7
MboII GAAGA 4 cut(s) 178, 187, 640, 652
MflI RGATCY 1 cut(s) 7
MhlI GDGCHC 2 cut(s) 186, 473
MluCI AATT 1 cut(s) 258
MlyI GAGTC 1 cut(s) 595
MmeI TCCRAC 2 cut(s) 423, 631
MnlI CCTC 9 cut(s) 7, 163, 330, 415, 446, 515, 518, 643, 658
MreI CGCCGGCG 1 cut(s) 360
MroNI GCCGGC 2 cut(s) 47, 360
MspA1I CMGCKG 1 cut(s) 450
MspI CCGG 3 cut(s) 48, 361, 419
MspR9I CCNGG 3 cut(s) 122, 496, 560
Mva1269I GAATGC 1 cut(s) 656
MvaI CCWGG 3 cut(s) 122, 496, 560
MwoI GCNNNNNNNGC 2 cut(s) 28, 456
NaeI GCCGGC 2 cut(s) 49, 362
NdeII GATC 1 cut(s) 7
NgoMIV GCCGGC 2 cut(s) 47, 360
NlaIII CATG 3 cut(s) 161, 585, 681
NlaIV GGNNCC 3 cut(s) 353, 470, 506
PciSI GCTCTTC 1 cut(s) 191
PcsI WCGNNNNNNNCGW 2 cut(s) 364, 660
PctI GAATGC 1 cut(s) 656
PdiI GCCGGC 2 cut(s) 49, 362
PkrI GCNGC 3 cut(s) 33, 481, 670
PleI GAGTC 1 cut(s) 595
PpsI GAGTC 1 cut(s) 595
PpuMI RGGWCCY 1 cut(s) 505
Psp124BI GAGCTC 1 cut(s) 186
Psp5II RGGWCCY 1 cut(s) 505
Psp6I CCWGG 3 cut(s) 120, 494, 558
PspFI CCCAGC 2 cut(s) 34, 464
PspGI CCWGG 3 cut(s) 120, 494, 558
PspN4I GGNNCC 3 cut(s) 353, 470, 506
PspPI GGNCC 3 cut(s) 118, 505, 674
PspPPI RGGWCCY 1 cut(s) 505
PsuI RGATCY 1 cut(s) 7
RsaI GTAC 3 cut(s) 91, 114, 312
RsaNI GTAC 3 cut(s) 90, 113, 311
SacI GAGCTC 1 cut(s) 186
SapI GCTCTTC 1 cut(s) 191
SatI GCNGC 3 cut(s) 32, 480, 669
Sau3AI GATC 1 cut(s) 7
Sau96I GGNCC 3 cut(s) 118, 505, 674
SchI GAGTC 1 cut(s) 595
ScrFI CCNGG 3 cut(s) 122, 496, 560
SduI GDGCHC 2 cut(s) 186, 473
SgrAI CRCCGGYG 1 cut(s) 360
SinI GGWCC 2 cut(s) 118, 505
SmlI CTYRAG 1 cut(s) 11
SmoI CTYRAG 1 cut(s) 11
Sse9I AATT 1 cut(s) 258
SsiI CCGC 2 cut(s) 31, 450
SstI GAGCTC 1 cut(s) 186
StyD4I CCNGG 3 cut(s) 120, 494, 558
TaqI TCGA 1 cut(s) 663
TasI AATT 1 cut(s) 258
TatI WGTACW 2 cut(s) 89, 112
TauI GCSGC 1 cut(s) 34
TscAI CASTG 3 cut(s) 115, 412, 466
TseI GCWGC 2 cut(s) 479, 668
TspRI CASTG 3 cut(s) 115, 412, 466
VpaK11BI GGWCC 2 cut(s) 118, 505
XmiI GTMKAC 3 cut(s) 78, 426, 438
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.