MD07G1251200.v1.1

Disease resistance protein RPP8-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0001473
Physical Location & Seq
Reverse (-)
31948274 .. 31951070
2797 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1251200.v1.1.491

Sequence Viewer

Length: 2646 bp
ATGGCAGAAGCTGTTGTTATTTTTGTTCTGGGGAGGGTTGCTGATGTACTTGCTGAAATCCAACTTCCCAAGGATGTTCGCCCGGAAATCCTGCGGCTGAGAGATGAGCTGAAGCGGATGCAGTGCTTTTTAAAAGATGCAGATGCAAAGCAAGAAGACGATCTGCAGGTGCGCAATTGGGTTTCTGATGTCAGAAATCTTGCTTATGATGCTGAAGACCTCATTGACACATACATCCTCAAAGTTTCATCCTACAAGTTCAAGAAATGGAACTTTGTTAAGAGATATGTTTCCACTCTCAAGGCCCGTTCCAAGATTGGGAAAGACCTTGTATTTCTCCGCACAAGAATCTCTGACGTTTCCATCAGCCACGAGGCATACAACATAAGAAGTATTGGTGAAGGAACAAGTCATGCCAATGAAGGACTACTGAAGCTGCGGCGATCAACTCCTCGTGGCCAGGATAAGGATATTGTTGGTCTGGAGGAAGATATAGCAACTCTGGTTGCACGACTTGTCTCGGAGGATCAGTGGCGTGCAATTTCAATAGTAGGCATGGGTGGTATCGGTAAGACCACTTGTGCTAAGGAAGTCTATAATCATGCTGACATTCAAACTTGTTTCGATCGCCGTGCTTGGGTCTATGTATCCCAACAGTTCAGAACAAGAGACGTACTGCAGAGCATTATAAAACAAGTTTCAACAAGAACAAAAGACATGGCGAAGCTGGGAGAAGACGAGTTGGAAGAAATGCTCTATAAACTTTTGGAAGGGAGACGCTATCTGATTGTTTTAGATGATATATGGAGCACCAGCGCTTTTGACAGTCTTGCAAAGGTCTTCCCGAACAATCACAGTGGAAGTAAATTATTTCTAACAACTCGCAACAACAATGTCGCTTTGCATGCCGATACCCAGAGCCTTCCCCATGAACTGCGATTTCGTAGCAAAGAGGACAGTTGGAAGTTGTTATGCCGAAAAGCATTCACTGGAAGTATTGAGGGAATGTGTACTCCACAATTGGAGGAAATCGGAAAGGAAATTGTGGAGAAATGTGCTGGTCTACCGCTAGCCATCGTTGTGTTGGGGGGCATGCTTTCAAGGAAAAGAAGGCTGAGTGAATGGCAAAGGGTTCTGAACTCCATCAGCGCACTGTTAGCCCGTGGTCCGAATGCTGTATCGGCAATACTAGCTTTGAGCTATTATGACTTGCCTTATTACCTTAAATTTTGTTTTCTCTACTTGGGGTTATTTCCTGAGGACTACTTATTCTCTGCACGTAAACTTTTTCGGCTATGGATTGCAGAGGGCTTGATCCCATATTACGATGGAAGGATGGAGGAACTTGCAGAGGAATATCTGAATGAGCTGATTGATAGAAACATGGTTCAAGCAGCGAGGTTGAGTGCAAATGATAGGGTGAAACACTGCCGATTGCACGACTTAATGCGAGACCTCTGTATCTCAAAGGCTAAGTCAGTGGAATTTCTTTATATTCATTCGAATCTGAAGTGCAGTATCTTTCAGCCATTACAATGCTCTCACTCTCGAGCCCGTCATCATGCTGTCTACTCCGGCTATATTTCCTCCCTGGATGAGTCAACTCCAAACCTCCGATCACTCCTCTTCTTTAAGTTTGAACAAATTGGAAGTGAAATTTCGCTTTCTCATGTCTGCAGAAGTTTTAAACTCCTTAGAGTCCTGGAATTAGAAGATGTGAAGTCATGGGGAATTCCAAGAGCAATCGGAGAAATGATCCATTTGAAGTACTTGGGGTTAAGGCATTGCTCTATAGGCTCGCTTCCAGAAGAAATAGGATGGTTGTCCAACTTGCAGACTCTTGATATTCTTGAAAATGTCAAGATTATGAGGGTTCCTGATGTGTTGTGGAAGATGAAAAGCTTACGCCATCTCTACATGTATCTTCCTCCATTCACTGGCAAGTTGCGAATTGACACTCTCCAAGACCTCCAGACTTTGGCAGGCATAAATGTTGATAGTTTGAGAGGAATAAACTCTGCCAATTTAATCAGTCTTCGGAAGTTGAGCTTGACTGGAAGCATCATCACAACCAGTGCAGAGATTTTTGGTTCCTTAGCCAATCTTCCGAACCTCCACTCCTTGTCCTTAACGTCTATAGATACTTTGTTTCCAACACTCTCTGCACTTTCTTGTCTTCGCTCTGTCTTTAAGTTGCATCTGAGTGGAGGGATAAGAAAGCTACCTAACACACATGAATTCCCTCCAAATCTCAACCAGTTGATCTTGCATCAATCCCGTCTTGAAAACAATCCTTTGGAGATACTTGAGAAGCTGCCTTATCTATTTGTTCTTAGACTAAAACATTCTTCTTACCGTGGAAAAAAACTGAAATTTTCTGCCAACGGCTTTCCTCAACTCGAGTATCTAGAGCTTGAATTCTTGGATTCTTTGGAAGAGTTGGAAGTTGAAGAAAGTGCAACGCCGAAGCTGAGGAGTTTGCAAATCACCTACTGCAAAAAGTTATGGATGCTCCCTGAAGAAATTAAGTCATTAACTACTCTCCAAGAGTTGGTGTTCGAAGGGATGCCAAGAAGATTCATAGATAGGCTGCAAGGAGAAGACCGTCACAAAGTCCAGCATGTTCCCTCTATCATAAAGTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

882

Amino Acids

100.99

Weight (kDa)

8.86

Isoelectric Point (pI)

44.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 5 - 91 3.1e-24 Rx N-terminal domain
NB-ARC PF00931 162 - 331 1e-46 NB-ARC domain
WHD_DRP PF23559 417 - 487 2.8e-20 Disease resistance protein Winged helix domain
LRR_14 PF23598 549 - 832 5.7e-34 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 689
AarI CACCTGC 1 cut(s) 157
Acc16I TGCGCA 1 cut(s) 173
Acc36I ACCTGC 1 cut(s) 157
AccB7I CCANNNNNTGG 3 cut(s) 1937, 1978, 2551
AccI GTMKAC 2 cut(s) 1063, 1569
AciI CCGC 5 cut(s) 94, 115, 340, 439, 1067
AclWI GGATC 3 cut(s) 534, 1309, 1750
AcoI YGGCCR 1 cut(s) 457
AcsI RAATTY 7 cut(s) 1226, 1484, 1656, 1731, 2237, 2372, 2417
AcuI CTGAAG 5 cut(s) 131, 234, 452, 1529, 2538
AfaI GTAC 4 cut(s) 48, 675, 1012, 1769
AfeI AGCGCT 1 cut(s) 817
AfiI CCNNNNNNNGG 6 cut(s) 318, 466, 637, 1937, 1978, 2551
AflIII ACRYGT 1 cut(s) 1917
AjnI CCWGG 3 cut(s) 459, 1590, 1701
AloI GAACNNNNNNTCC 2 cut(s) 2102, 2134
Alw21I GWGCWC 1 cut(s) 812
Alw26I GTCTC 4 cut(s) 523, 663, 769, 1446
AlwI GGATC 3 cut(s) 534, 1309, 1750
AlwNI CAGNNNCTG 1 cut(s) 11
Ama87I CYCGRG 2 cut(s) 1548, 2399
Aor51HI AGCGCT 1 cut(s) 817
AoxI GGCC 2 cut(s) 303, 457
ApeKI GCWGC 4 cut(s) 436, 1394, 2314, 2590
ApoI RAATTY 7 cut(s) 1226, 1484, 1656, 1731, 2237, 2372, 2417
ArsI GACNNNNNNTTYG 2 cut(s) 1460, 1492
AspLEI GCGC 3 cut(s) 174, 818, 1151
AspS9I GGNCC 2 cut(s) 304, 1166
AsuC2I CCSGG 1 cut(s) 83
AsuHPI GGTGA 3 cut(s) 410, 1432, 2479
AsuII TTCGAA 2 cut(s) 1502, 2559
AsuNHI GCTAGC 1 cut(s) 1069
AvaI CYCGRG 2 cut(s) 1548, 2399
AvaII GGWCC 1 cut(s) 1166
AxyI CCTNAGG 1 cut(s) 1257
BaeI ACNNNNGTAYC 4 cut(s) 1444, 1477, 2387, 2420
BalI TGGCCA 1 cut(s) 459
BanII GRGCYC 1 cut(s) 1555
BauI CACGAG 2 cut(s) 371, 453
BbsI GAAGAC 7 cut(s) 162, 222, 741, 832, 2027, 2168, 2607
Bbv12I GWGCWC 1 cut(s) 812
BbvCI CCTCAGC 1 cut(s) 2471
BbvI GCAGC 4 cut(s) 423, 1406, 2301, 2577
BccI CCATC 7 cut(s) 371, 1082, 1151, 1322, 1330, 1812, 1917
BceAI ACGGC 2 cut(s) 615, 2401
BcgI CGANNNNNNTGC 2 cut(s) 614, 648
BciT130I CCWGG 3 cut(s) 461, 1592, 1703
BciVI GTATCC 1 cut(s) 658
BcnI CCSGG 1 cut(s) 83
BcoDI GTCTC 4 cut(s) 523, 663, 769, 1446
BfaI CTAG 3 cut(s) 1070, 1190, 2408
BfmI CTRYAG 5 cut(s) 164, 677, 1675, 1791, 2136
BfoI RGCGCY 1 cut(s) 819
BfuAI ACCTGC 1 cut(s) 157
BfuI GTATCC 1 cut(s) 658
BisI GCNGC 6 cut(s) 95, 437, 440, 1395, 2315, 2591
BlsI GCNGC 6 cut(s) 96, 438, 441, 1396, 2316, 2592
BmcAI AGTACT 1 cut(s) 1769
Bme1390I CCNGG 4 cut(s) 83, 461, 1592, 1703
Bme18I GGWCC 1 cut(s) 1166
BmeT110I CYCGRG 2 cut(s) 1548, 2399
BmgT120I GGNCC 2 cut(s) 304, 1166
BmiI GGNNCC 2 cut(s) 1875, 2092
BmrFI CCNGG 4 cut(s) 83, 461, 1592, 1703
BmsI GCATC 9 cut(s) 108, 127, 133, 199, 2070, 2206, 2278, 2499, 2556
BmtI GCTAGC 1 cut(s) 1073
BpiI GAAGAC 7 cut(s) 162, 222, 741, 832, 2027, 2168, 2607
BpmI CTGGAG 2 cut(s) 503, 1955
Bpu10I CCTNAGC 3 cut(s) 585, 2095, 2471
Bpu14I TTCGAA 2 cut(s) 1502, 2559
BpuEI CTTGAG 2 cut(s) 284, 2327
BpuMI CCSGG 1 cut(s) 83
BsaAI YACGTR 1 cut(s) 1280
BsaI GGTCTC 1 cut(s) 1446
BsaJI CCNNGG 4 cut(s) 69, 1162, 1590, 2356
BsaXI ACNNNNNCTCC 4 cut(s) 515, 545, 2102, 2132
Bsc4I CCNNNNNNNGG 6 cut(s) 318, 466, 637, 1937, 1978, 2551
Bse1I ACTGG 5 cut(s) 994, 1942, 2059, 2073, 2257
Bse21I CCTNAGG 1 cut(s) 1257
Bse3DI GCAATG 1 cut(s) 1783
BseBI CCWGG 3 cut(s) 461, 1592, 1703
BseDI CCNNGG 4 cut(s) 69, 1162, 1590, 2356
BseGI GGATG 9 cut(s) 79, 123, 234, 248, 1341, 1600, 1823, 2514, 2571
BseLI CCNNNNNNNGG 6 cut(s) 318, 466, 637, 1937, 1978, 2551
BseMI GCAATG 1 cut(s) 1783
BseMII CTCAG 5 cut(s) 89, 1106, 1248, 2192, 2462
BseNI ACTGG 5 cut(s) 994, 1942, 2059, 2073, 2257
BseRI GAGGAG 3 cut(s) 441, 1613, 2488
BseXI GCAGC 4 cut(s) 423, 1406, 2301, 2577
BseYI CCCAGC 1 cut(s) 727
BsgI GTGCAG 4 cut(s) 1260, 1534, 2097, 2148
Bsh1285I CGRYCG 1 cut(s) 628
BshFI GGCC 2 cut(s) 305, 459
BsiEI CGRYCG 1 cut(s) 628
BsiHKAI GWGCWC 1 cut(s) 812
BsiHKCI CYCGRG 2 cut(s) 1548, 2399
BsiSI CCGG 2 cut(s) 83, 1575
BslI CCNNNNNNNGG 6 cut(s) 318, 466, 637, 1937, 1978, 2551
BsmAI GTCTC 4 cut(s) 523, 663, 769, 1446
BsmBI CGTCTC 2 cut(s) 663, 769
BsmI GAATGC 2 cut(s) 983, 1177
BsnI GGCC 2 cut(s) 305, 459
Bso31I GGTCTC 1 cut(s) 1446
BsoBI CYCGRG 2 cut(s) 1548, 2399
Bsp119I TTCGAA 2 cut(s) 1502, 2559
Bsp1286I GDGCHC 2 cut(s) 812, 1555
Bsp143I GATC 8 cut(s) 160, 443, 526, 625, 1314, 1616, 1755, 2262
BspACI CCGC 5 cut(s) 94, 115, 340, 439, 1067
BspANI GGCC 2 cut(s) 305, 459
BspCNI CTCAG 5 cut(s) 90, 1107, 1249, 2193, 2463
BspLI GGNNCC 2 cut(s) 1875, 2092
BspMAI CTGCAG 3 cut(s) 168, 681, 1679
BspMI ACCTGC 1 cut(s) 157
BspOI GCTAGC 1 cut(s) 1073
BspPI GGATC 3 cut(s) 534, 1309, 1750
BspT104I TTCGAA 2 cut(s) 1502, 2559
BspTNI GGTCTC 1 cut(s) 1446
BsrDI GCAATG 1 cut(s) 1783
BsrI ACTGG 5 cut(s) 994, 1942, 2059, 2073, 2257
BssECI CCNNGG 4 cut(s) 69, 1162, 1590, 2356
BssMI GATC 8 cut(s) 160, 443, 526, 625, 1314, 1616, 1755, 2262
BssSI CACGAG 2 cut(s) 371, 453
BssT1I CCWWGG 1 cut(s) 69
Bst2BI CACGAG 2 cut(s) 371, 453
Bst2UI CCWGG 3 cut(s) 461, 1592, 1703
Bst4CI ACNGT 7 cut(s) 657, 827, 857, 959, 1155, 2357, 2606
Bst6I CTCTTC 2 cut(s) 1631, 2430
BstBAI YACGTR 1 cut(s) 1280
BstBI TTCGAA 2 cut(s) 1502, 2559
BstC8I GCNNGC 6 cut(s) 537, 906, 1071, 1094, 1799, 1984
BstDSI CCRYGG 2 cut(s) 1162, 2356
BstF5I GGATG 9 cut(s) 79, 123, 234, 248, 1341, 1600, 1823, 2514, 2571
BstH2I RGCGCY 1 cut(s) 819
BstHHI GCGC 3 cut(s) 174, 818, 1151
BstKTI GATC 8 cut(s) 163, 446, 529, 628, 1317, 1619, 1758, 2265
BstMAI GTCTC 4 cut(s) 523, 663, 769, 1446
BstMBI GATC 8 cut(s) 160, 443, 526, 625, 1314, 1616, 1755, 2262
BstMCI CGRYCG 1 cut(s) 628
BstMWI GCNNNNNNNGC 6 cut(s) 209, 905, 1157, 1181, 1190, 1794
BstNI CCWGG 3 cut(s) 461, 1592, 1703
BstNSI RCATGY 4 cut(s) 908, 1096, 1921, 2624
BstSCI CCNGG 4 cut(s) 81, 459, 1590, 1701
BstSFI CTRYAG 5 cut(s) 164, 677, 1675, 1791, 2136
BstV1I GCAGC 4 cut(s) 423, 1406, 2301, 2577
BstV2I GAAGAC 7 cut(s) 162, 222, 741, 832, 2027, 2168, 2607
Bsu36I CCTNAGG 1 cut(s) 1257
BsuI GTATCC 1 cut(s) 658
BsuRI GGCC 2 cut(s) 305, 459
BtgI CCRYGG 2 cut(s) 1162, 2356
BtsCI GGATG 9 cut(s) 79, 123, 234, 248, 1341, 1600, 1823, 2514, 2571
BtsI GCAGTG 2 cut(s) 128, 1426
BtsIMutI CAGTG 9 cut(s) 128, 536, 862, 987, 1151, 1426, 1485, 1935, 2080
BveI ACCTGC 1 cut(s) 157
Cac8I GCNNGC 6 cut(s) 537, 906, 1071, 1094, 1799, 1984
CaiI CAGNNNCTG 1 cut(s) 11
CfoI GCGC 3 cut(s) 174, 818, 1151
Cfr13I GGNCC 2 cut(s) 304, 1166
CseI GACGC 1 cut(s) 786
Csp6I GTAC 4 cut(s) 47, 674, 1011, 1768
CspCI CAANNNNNGTGG 2 cut(s) 838, 873
CviQI GTAC 4 cut(s) 47, 674, 1011, 1768
DpnI GATC 8 cut(s) 162, 445, 528, 627, 1316, 1618, 1757, 2264
DpnII GATC 8 cut(s) 160, 443, 526, 625, 1314, 1616, 1755, 2262
DraI TTTAAA 2 cut(s) 132, 1687
EaeI YGGCCR 1 cut(s) 457
Eam1104I CTCTTC 2 cut(s) 1631, 2430
EarI CTCTTC 2 cut(s) 1631, 2430
Eco130I CCWWGG 1 cut(s) 69
Eco24I GRGCYC 1 cut(s) 1555
Eco31I GGTCTC 1 cut(s) 1446
Eco47I GGWCC 1 cut(s) 1166
Eco47III AGCGCT 1 cut(s) 817
Eco57I CTGAAG 5 cut(s) 131, 234, 452, 1529, 2538
Eco81I CCTNAGG 1 cut(s) 1257
Eco88I CYCGRG 2 cut(s) 1548, 2399
EcoRI GAATTC 3 cut(s) 1731, 2237, 2417
EcoRII CCWGG 3 cut(s) 459, 1590, 1701
EcoT14I CCWWGG 1 cut(s) 69
EcoT38I GRGCYC 1 cut(s) 1555
ErhI CCWWGG 1 cut(s) 69
Esp3I CGTCTC 2 cut(s) 663, 769
FalI AAGNNNNNCTT 2 cut(s) 2033, 2065
FblI GTMKAC 2 cut(s) 1063, 1569
Fnu4HI GCNGC 6 cut(s) 95, 437, 440, 1395, 2315, 2591
FokI GGATG 9 cut(s) 86, 130, 221, 235, 1348, 1607, 1830, 2521, 2578
FriOI GRGCYC 1 cut(s) 1555
Fsp4HI GCNGC 6 cut(s) 95, 437, 440, 1395, 2315, 2591
FspBI CTAG 3 cut(s) 1070, 1190, 2408
FspI TGCGCA 1 cut(s) 173
GlaI GCGC 3 cut(s) 173, 817, 1150
GluI GCNGC 6 cut(s) 95, 437, 440, 1395, 2315, 2591
GsaI CCCAGC 1 cut(s) 731
GsuI CTGGAG 2 cut(s) 503, 1955
HaeII RGCGCY 1 cut(s) 819
HaeIII GGCC 2 cut(s) 305, 459
HapII CCGG 2 cut(s) 83, 1575
HgaI GACGC 1 cut(s) 786
HhaI GCGC 3 cut(s) 174, 818, 1151
Hin6I GCGC 3 cut(s) 172, 816, 1149
HinP1I GCGC 3 cut(s) 172, 816, 1149
HincII GTYRAC 1 cut(s) 1602
HindII GTYRAC 1 cut(s) 1602
HindIII AAGCTT 1 cut(s) 1900
HinfI GANTC 7 cut(s) 348, 1504, 1598, 1698, 1837, 2426, 2577
HpaII CCGG 2 cut(s) 83, 1575
HphI GGTGA 3 cut(s) 410, 1432, 2479
Hpy166II GTNNAC 5 cut(s) 1011, 1064, 1283, 1570, 1602
Hpy8I GTNNAC 5 cut(s) 1011, 1064, 1283, 1570, 1602
HpyAV CCTTC 7 cut(s) 395, 416, 764, 932, 1104, 1326, 2555
HpyCH4III ACNGT 7 cut(s) 657, 827, 857, 959, 1155, 2357, 2606
HpyCH4IV ACGT 4 cut(s) 357, 672, 1279, 2132
HpyF10VI GCNNNNNNNGC 6 cut(s) 209, 905, 1157, 1181, 1190, 1794
HpySE526I ACGT 4 cut(s) 357, 672, 1279, 2132
HspAI GCGC 3 cut(s) 172, 816, 1149
Kzo9I GATC 8 cut(s) 160, 443, 526, 625, 1314, 1616, 1755, 2262
LmnI GCTCC 2 cut(s) 807, 2517
Lsp1109I GCAGC 4 cut(s) 423, 1406, 2301, 2577
LweI GCATC 9 cut(s) 108, 127, 133, 199, 2070, 2206, 2278, 2499, 2556
MaeI CTAG 3 cut(s) 1070, 1190, 2408
MaeII ACGT 4 cut(s) 357, 672, 1279, 2132
MaeIII GTNAC 1 cut(s) 2606
MalI GATC 8 cut(s) 162, 445, 528, 627, 1316, 1618, 1757, 2264
MboI GATC 8 cut(s) 160, 443, 526, 625, 1314, 1616, 1755, 2262
MfeI CAATTG 2 cut(s) 175, 1019
MhlI GDGCHC 2 cut(s) 812, 1555
MlsI TGGCCA 1 cut(s) 459
MluNI TGGCCA 1 cut(s) 459
MlyI GAGTC 3 cut(s) 1607, 1707, 1831
MmeI TCCRAC 6 cut(s) 85, 723, 941, 1851, 2177, 2421
Mox20I TGGCCA 1 cut(s) 459
MscI TGGCCA 1 cut(s) 459
MslI CAYNNNNRTG 4 cut(s) 417, 1079, 1534, 2202
Msp20I TGGCCA 1 cut(s) 459
MspI CCGG 2 cut(s) 83, 1575
MspR9I CCNGG 4 cut(s) 83, 461, 1592, 1703
MunI CAATTG 2 cut(s) 175, 1019
Mva1269I GAATGC 2 cut(s) 983, 1177
MvaI CCWGG 3 cut(s) 461, 1592, 1703
MwoI GCNNNNNNNGC 6 cut(s) 209, 905, 1157, 1181, 1190, 1794
NciI CCSGG 1 cut(s) 83
NdeII GATC 8 cut(s) 160, 443, 526, 625, 1314, 1616, 1755, 2262
NheI GCTAGC 1 cut(s) 1069
NlaIV GGNNCC 2 cut(s) 1875, 2092
NmuCI GTSAC 1 cut(s) 2606
NsbI TGCGCA 1 cut(s) 173
NspI RCATGY 4 cut(s) 908, 1096, 1921, 2624
NspV TTCGAA 2 cut(s) 1502, 2559
PaeI GCATGC 2 cut(s) 908, 1096
PaeR7I CTCGAG 2 cut(s) 1548, 2399
PaqCI CACCTGC 1 cut(s) 157
PciI ACATGT 1 cut(s) 1917
PctI GAATGC 2 cut(s) 983, 1177
PfeI GAWTC 4 cut(s) 348, 1504, 2426, 2577
PflMI CCANNNNNTGG 3 cut(s) 1937, 1978, 2551
PfoI TCCNGGA 1 cut(s) 1701
PkrI GCNGC 6 cut(s) 96, 438, 441, 1396, 2316, 2592
Ple19I CGATCG 1 cut(s) 628
PleI GAGTC 3 cut(s) 1606, 1706, 1831
PpsI GAGTC 3 cut(s) 1606, 1706, 1831
Ppu21I YACGTR 1 cut(s) 1280
PscI ACATGT 1 cut(s) 1917
PsiI TTATAA 1 cut(s) 689
Psp6I CCWGG 3 cut(s) 459, 1590, 1701
PspFI CCCAGC 1 cut(s) 727
PspGI CCWGG 3 cut(s) 459, 1590, 1701
PspN4I GGNNCC 2 cut(s) 1875, 2092
PspPI GGNCC 2 cut(s) 304, 1166
PspXI VCTCGAGB 1 cut(s) 2399
PstI CTGCAG 3 cut(s) 168, 681, 1679
PstNI CAGNNNCTG 1 cut(s) 11
PvuI CGATCG 1 cut(s) 628
RsaI GTAC 4 cut(s) 48, 675, 1012, 1769
RsaNI GTAC 4 cut(s) 47, 674, 1011, 1768
RseI CAYNNNNRTG 4 cut(s) 417, 1079, 1534, 2202
SatI GCNGC 6 cut(s) 95, 437, 440, 1395, 2315, 2591
Sau3AI GATC 8 cut(s) 160, 443, 526, 625, 1314, 1616, 1755, 2262
Sau96I GGNCC 2 cut(s) 304, 1166
ScaI AGTACT 1 cut(s) 1769
SchI GAGTC 3 cut(s) 1607, 1707, 1831
ScrFI CCNGG 4 cut(s) 83, 461, 1592, 1703
SduI GDGCHC 2 cut(s) 812, 1555
SfaNI GCATC 9 cut(s) 108, 127, 133, 199, 2070, 2206, 2278, 2499, 2556
SfcI CTRYAG 5 cut(s) 164, 677, 1675, 1791, 2136
Sfr274I CTCGAG 2 cut(s) 1548, 2399
SfuI TTCGAA 2 cut(s) 1502, 2559
SinI GGWCC 1 cut(s) 1166
SlaI CTCGAG 2 cut(s) 1548, 2399
SmiMI CAYNNNNRTG 4 cut(s) 417, 1079, 1534, 2202
SmlI CTYRAG 4 cut(s) 299, 1548, 2306, 2399
SmoI CTYRAG 4 cut(s) 299, 1548, 2306, 2399
SphI GCATGC 2 cut(s) 908, 1096
SsiI CCGC 5 cut(s) 94, 115, 340, 439, 1067
SspMI CTAG 3 cut(s) 1070, 1190, 2408
StyD4I CCNGG 4 cut(s) 81, 459, 1590, 1701
StyI CCWWGG 1 cut(s) 69
TaaI ACNGT 7 cut(s) 657, 827, 857, 959, 1155, 2357, 2606
TaiI ACGT 4 cut(s) 360, 675, 1282, 2135
TaqI TCGA 5 cut(s) 624, 1502, 1549, 2400, 2559
TatI WGTACW 3 cut(s) 46, 1010, 1767
TauI GCSGC 2 cut(s) 97, 442
TfiI GAWTC 4 cut(s) 348, 1504, 2426, 2577
TscAI CASTG 9 cut(s) 128, 536, 862, 994, 1158, 1433, 1485, 1942, 2080
TseFI GTSAC 1 cut(s) 2606
TseI GCWGC 4 cut(s) 436, 1394, 2314, 2590
Tsp45I GTSAC 1 cut(s) 2606
TspDTI ATGAA 7 cut(s) 237, 435, 945, 1487, 1910, 2250, 2569
TspRI CASTG 9 cut(s) 128, 536, 862, 994, 1158, 1433, 1485, 1942, 2080
Van91I CCANNNNNTGG 3 cut(s) 1937, 1978, 2551
VpaK11BI GGWCC 1 cut(s) 1166
XapI RAATTY 7 cut(s) 1226, 1484, 1656, 1731, 2237, 2372, 2417
XbaI TCTAGA 1 cut(s) 2407
XceI RCATGY 4 cut(s) 908, 1096, 1921, 2624
XcmI CCANNNNNNNNNTGG 1 cut(s) 1081
XhoI CTCGAG 2 cut(s) 1548, 2399
XmiI GTMKAC 2 cut(s) 1063, 1569
XspI CTAG 3 cut(s) 1070, 1190, 2408
ZrmI AGTACT 1 cut(s) 1769
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.