pycom07g22380

Disease resistance protein RPP8-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0001473
Physical Location & Seq
Reverse (-)
23739442 .. 23742087
2646 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g22380.1

Sequence Viewer

Length: 2646 bp
ATGGCAGAAGCTGTTGTTATTTTCGTTCTGGAAAGGGTTGCTGATGTACTTGGTGAAATCCAACTTCCCAAGGATGTTCGCCCGGAAATCCTGCGGCTGAGAGATGAGCTGAAGCGGATGCAGTGCTTTTTAAAAGATGCAGATGCAAAGCAAGAAGACGATTTGCAGGTGCGGAATTGGGTTTCTGATGTCAGAAATCTTGCTTATGATGCTGAAGACCTCATTGACACATACATCCTCAAAGTTGAGTCCTACAAGTACAAGAAATGGAACTTTGTTAAGAGATATGCTTCCACTCTCAAGGCCCGTTACAAGATTGGGAAAGACCTTGTATTTCTCCGCACAAGAATCTCTGACGTTTCCATCAGCCACGAAGCATACGGCATAAGAAGTACTGGTGAAGGAACGAGTCATGCCAATGAAGGACTACTGAAGCTGCGGCGATCAACTCCTCGTGGCCAGGATAAGGATATTGTTGGTCTGGAGGAAGATATAGCAACTCTGGTGGCACGACTTGTCTCGGAGGATCAATGGCGTGCAATTTCAATAGTAGGCATGGGTGGTATTGGTAAGACCACTTGTGCTAAGGAAGTCTATAATCATGCTGACATTCAAACTTGTTTCGATCGCCGTGCTTGGGTCTATGTATCCCAACAGTTCAGAACAAGAGACATACTTGTGAGCATTATAAAACAAGTTTCAACAAGAACAAAAGACACGGCGAAGTTGGGAGAAGACGAGTTGGAGGAAGTGCTCTATAAACTTTTGGAAGGGAGACGCTATCTGATTGTTTTAGATGATATATGGAGCACCGGCGCTTTTGACAGTCTTGCAAAGGCCTTCCCAAACAATCACAGTGGAAGTAAATTATTTCTTACAACTCGCAACAACAATGTCGCTTTGCATGCCGATGCCCAGAGCCTTCCCCATGAACTGCGATTTCGTAGCAAAGAGGACAGTTGGAAGTTGTTATGCCGGAAAGCATTCACTGAAAGTATTGAGAGAATGTGTCCTCCACAATTGGAGGAAATCGGAAAGGAAATTGTGGCGAAATGTGCTGGTCTACCGCTAGCCATCGTTGTGTTGGGGGGCATGCTTTCAAGGAAAAGAAGGCTGAGTGAATGGCAAAGGGTTCTGAACTCCATCCGCGCACTGTTAGCCCGTGGTCCGAATGCTGTGTCGGCAATACTAGCTTTAAGCTATTATGACTTGCCTTATTACCTTAAATTTTGTTTTCTCTACTTGGGGTTATTTCCTGAGGACTATTTATTCTCTGCACGTAAACTTTTTCGGCTATGGATTGCAGAAGGCTTGATCCCATATTACGATGGAAGGATGGAGGACCTTGCAGAGGAATATCTGAATGAGCTGATTGATAGAAACATGGTTCAAGCAGCAAGGTTGAGTGCAAATGATAGGGTGAAACACTGCCGATTGCACGACTTAATGCGAGACCTCTGTATCTCAAAGGCTAAGTCAGTGGAATTTCTTTATATTCATTCGAATCTGAAGTACAGTATCTTCAGCCCATTTCCATGTTCTCACTCTCGAGCCCGTCATCATGCTATCTACTCCGGCTTTTCTTCATCCCCGGATGAGTCAACTCCAAGCCTCCGATCGCTCCTCTTCTTTAAGGTTCGACAAATTGAAAGTGGAATTTCTCTTTCTGATGTCTGCAGACGTTTTAAACTCCTTAGAGTCCTGGAATTAGAAGATATGAAGTTGAGGACAATTCCAAGCGCAATCGGAGAAATGATCCATTTGAAGTACTTGGGGTTAAGGCATTGCTCTATAGGGTCGGTGCCAGAAGAAATAGGATGGTTGTCCAACTTGCAGACTCTTGATATTCTTGAAAATGCCAATATTTTGAGGTTTCCTGATGTGTTATGGAAGATGAAAAGCTTACGCCATCTTTACATGTACGTTCATCCATTAGTTGGCAAGTGGCGAATTGATACTCTCCAACACCTCCAGACTTTGGTGGGCATAAACGTTGATAGCTTGAGAGGAATAAACTCCGCCAATTTAATCAGTCTTCGGAAGTTGAGATTGACTGGAAGCTTCGCCACAAACCGTGCAGAAATTTTTGATTCTTTAGTCAATCTTCTGAACCTCCACTCCTTGTCCTTAAAGTCTATGGATTATCTGTTTCCATCCCTCTCTGCGCTTTCTTCTCTTCGCCATGTCATTAAGTTGCATCTGAGCGGAGGGATAAGTAAGCTACCTAACCCACATGAATTCCCTCCAAATCTCAACCAGTTGATCTTGCATCAATCCCGTCTTGAAAACAATCCTTTGGAGATCCTTGAGAAGCTGCCTTATCTATTTGTTCTTAGACTAAAACATTCTTCTTACCATGGAAAAAAACTGAAATTTTCTGCCAACGGCTTTCCACAACTCGAGTATCTAGAGCTTGAATTCTTGGATTCTTTGGAAGAGTTGGAAGTTGAAGAAAGTGCAATGCCGAAGCTTAGGAGTTTACAGATCACTGACTGCCAGCAGTTACGGATGCTCCCTGAAGAAATTAAGTGCATAACTACTCTCCAAGAGTTGGTGTTCGAAGGGATGCCAAGAAGATTCATAGATAGGCTGCAAGGAGAAGACCATCACAAAGTCCAGCATGTTCCCTCTATCATAAAGTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

882

Amino Acids

101.34

Weight (kDa)

8.83

Isoelectric Point (pI)

45.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 5 - 90 2.5e-24 Rx N-terminal domain
NB-ARC PF00931 162 - 332 1.8e-45 NB-ARC domain
WHD_DRP PF23559 417 - 487 2.1e-20 Disease resistance protein Winged helix domain
LRR_14 PF23598 550 - 832 1.8e-35 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 689
AarI CACCTGC 1 cut(s) 157
Acc36I ACCTGC 1 cut(s) 157
AccB1I GGYRCC 1 cut(s) 1801
AccB7I CCANNNNNTGG 3 cut(s) 1937, 1978, 2551
AccBSI CCGCTC 1 cut(s) 2205
AccI GTMKAC 1 cut(s) 1063
AccII CGCG 1 cut(s) 1149
AciI CCGC 9 cut(s) 94, 115, 172, 340, 439, 1067, 1147, 2019, 2205
AclI AACGTT 1 cut(s) 1992
AclWI GGATC 4 cut(s) 534, 1309, 1750, 2296
AcoI YGGCCR 1 cut(s) 457
AcsI RAATTY 7 cut(s) 1226, 1484, 1656, 2082, 2237, 2372, 2417
AcuI CTGAAG 6 cut(s) 131, 234, 452, 1507, 1529, 2538
AfaI GTAC 6 cut(s) 48, 260, 394, 1514, 1769, 1922
AfiI CCNNNNNNNGG 6 cut(s) 466, 637, 1351, 1937, 1978, 2551
AflIII ACRYGT 1 cut(s) 1917
AjnI CCWGG 2 cut(s) 459, 1701
AloI GAACNNNNNNTCC 2 cut(s) 2102, 2134
Alw21I GWGCWC 2 cut(s) 756, 812
Alw26I GTCTC 4 cut(s) 523, 663, 769, 1446
AlwI GGATC 4 cut(s) 534, 1309, 1750, 2296
AlwNI CAGNNNCTG 1 cut(s) 11
Ama87I CYCGRG 2 cut(s) 1548, 2399
AoxI GGCC 3 cut(s) 303, 457, 837
ApeKI GCWGC 4 cut(s) 436, 1394, 2314, 2590
ApoI RAATTY 7 cut(s) 1226, 1484, 1656, 2082, 2237, 2372, 2417
ArsI GACNNNNNNTTYG 4 cut(s) 1163, 1195, 1460, 1492
Asp700I GAANNNNTTC 1 cut(s) 983
AspLEI GCGC 4 cut(s) 818, 1151, 1742, 2167
AspS9I GGNCC 3 cut(s) 304, 1166, 1342
AsuC2I CCSGG 2 cut(s) 83, 1592
AsuHPI GGTGA 3 cut(s) 65, 410, 1432
AsuII TTCGAA 2 cut(s) 1502, 2559
AsuNHI GCTAGC 1 cut(s) 1069
AvaI CYCGRG 2 cut(s) 1548, 2399
AvaII GGWCC 2 cut(s) 1166, 1342
AxyI CCTNAGG 1 cut(s) 1257
BaeI ACNNNNGTAYC 4 cut(s) 1444, 1477, 2387, 2420
BalI TGGCCA 1 cut(s) 459
BanI GGYRCC 1 cut(s) 1801
BanII GRGCYC 1 cut(s) 1555
BarI GAAGNNNNNNTAC 2 cut(s) 1505, 1537
BauI CACGAG 1 cut(s) 453
BbsI GAAGAC 5 cut(s) 162, 222, 741, 2027, 2607
Bbv12I GWGCWC 2 cut(s) 756, 812
BbvI GCAGC 4 cut(s) 423, 1406, 2301, 2577
BccI CCATC 9 cut(s) 371, 1082, 1151, 1322, 1330, 1812, 1917, 2161, 2613
BceAI ACGGC 4 cut(s) 397, 615, 735, 2401
BcgI CGANNNNNNTGC 2 cut(s) 614, 648
BciT130I CCWGG 2 cut(s) 461, 1703
BciVI GTATCC 1 cut(s) 658
BcnI CCSGG 2 cut(s) 83, 1592
BcoDI GTCTC 4 cut(s) 523, 663, 769, 1446
BfaI CTAG 3 cut(s) 1070, 1190, 2408
BfmI CTRYAG 2 cut(s) 1675, 1791
BfoI RGCGCY 1 cut(s) 819
BfuAI ACCTGC 1 cut(s) 157
BfuI GTATCC 1 cut(s) 658
BisI GCNGC 6 cut(s) 95, 437, 440, 1395, 2315, 2591
BlsI GCNGC 6 cut(s) 96, 438, 441, 1396, 2316, 2592
BmcAI AGTACT 2 cut(s) 394, 1769
Bme1390I CCNGG 4 cut(s) 83, 461, 1592, 1703
Bme18I GGWCC 2 cut(s) 1166, 1342
BmeT110I CYCGRG 2 cut(s) 1548, 2399
BmgT120I GGNCC 3 cut(s) 304, 1166, 1342
BmiI GGNNCC 1 cut(s) 1803
BmrFI CCNGG 4 cut(s) 83, 461, 1592, 1703
BmsI GCATC 9 cut(s) 108, 127, 133, 199, 901, 2206, 2278, 2499, 2556
BmtI GCTAGC 1 cut(s) 1073
BpiI GAAGAC 5 cut(s) 162, 222, 741, 2027, 2607
BpmI CTGGAG 2 cut(s) 503, 1955
Bpu10I CCTNAGC 2 cut(s) 585, 2471
Bpu14I TTCGAA 2 cut(s) 1502, 2559
BpuEI CTTGAG 3 cut(s) 284, 2023, 2327
BpuMI CCSGG 2 cut(s) 83, 1592
BsaAI YACGTR 1 cut(s) 1280
BsaI GGTCTC 1 cut(s) 1446
BsaJI CCNNGG 4 cut(s) 69, 1162, 1590, 2356
BsaXI ACNNNNNCTCC 4 cut(s) 2102, 2132, 2496, 2526
Bsc4I CCNNNNNNNGG 6 cut(s) 466, 637, 1351, 1937, 1978, 2551
Bse118I RCCGGY 1 cut(s) 812
Bse1I ACTGG 3 cut(s) 400, 2059, 2257
Bse21I CCTNAGG 1 cut(s) 1257
Bse3DI GCAATG 2 cut(s) 1783, 2466
BseBI CCWGG 2 cut(s) 461, 1703
BseDI CCNNGG 4 cut(s) 69, 1162, 1590, 2356
BseLI CCNNNNNNNGG 6 cut(s) 466, 637, 1351, 1937, 1978, 2551
BseMI GCAATG 2 cut(s) 1783, 2466
BseMII CTCAG 4 cut(s) 89, 1106, 1248, 2192
BseNI ACTGG 3 cut(s) 400, 2059, 2257
BseRI GAGGAG 2 cut(s) 441, 1613
BseXI GCAGC 4 cut(s) 423, 1406, 2301, 2577
BsgI GTGCAG 2 cut(s) 1260, 2097
Bsh1236I CGCG 1 cut(s) 1149
Bsh1285I CGRYCG 2 cut(s) 628, 1619
BshFI GGCC 3 cut(s) 305, 459, 839
BshNI GGYRCC 1 cut(s) 1801
BsiEI CGRYCG 2 cut(s) 628, 1619
BsiHKAI GWGCWC 2 cut(s) 756, 812
BsiHKCI CYCGRG 2 cut(s) 1548, 2399
BsiSI CCGG 5 cut(s) 83, 813, 976, 1575, 1592
BslI CCNNNNNNNGG 6 cut(s) 466, 637, 1351, 1937, 1978, 2551
BsmAI GTCTC 4 cut(s) 523, 663, 769, 1446
BsmBI CGTCTC 1 cut(s) 769
BsmI GAATGC 2 cut(s) 983, 1177
BsnI GGCC 3 cut(s) 305, 459, 839
Bso31I GGTCTC 1 cut(s) 1446
BsoBI CYCGRG 2 cut(s) 1548, 2399
Bsp119I TTCGAA 2 cut(s) 1502, 2559
Bsp1286I GDGCHC 3 cut(s) 756, 812, 1555
Bsp143I GATC 9 cut(s) 443, 526, 625, 1314, 1616, 1755, 2262, 2301, 2484
Bsp19I CCATGG 1 cut(s) 2356
BspACI CCGC 9 cut(s) 94, 115, 172, 340, 439, 1067, 1147, 2019, 2205
BspANI GGCC 3 cut(s) 305, 459, 839
BspCNI CTCAG 4 cut(s) 90, 1107, 1249, 2193
BspFNI CGCG 1 cut(s) 1149
BspLI GGNNCC 1 cut(s) 1803
BspMAI CTGCAG 1 cut(s) 1679
BspMI ACCTGC 1 cut(s) 157
BspOI GCTAGC 1 cut(s) 1073
BspPI GGATC 4 cut(s) 534, 1309, 1750, 2296
BspT104I TTCGAA 2 cut(s) 1502, 2559
BspT107I GGYRCC 1 cut(s) 1801
BspTNI GGTCTC 1 cut(s) 1446
BsrBI CCGCTC 1 cut(s) 2205
BsrDI GCAATG 2 cut(s) 1783, 2466
BsrFI RCCGGY 1 cut(s) 812
BsrI ACTGG 3 cut(s) 400, 2059, 2257
BssAI RCCGGY 1 cut(s) 812
BssECI CCNNGG 4 cut(s) 69, 1162, 1590, 2356
BssMI GATC 9 cut(s) 443, 526, 625, 1314, 1616, 1755, 2262, 2301, 2484
BssSI CACGAG 1 cut(s) 453
BssT1I CCWWGG 2 cut(s) 69, 2356
Bst2BI CACGAG 1 cut(s) 453
Bst2UI CCWGG 2 cut(s) 461, 1703
Bst4CI ACNGT 7 cut(s) 657, 827, 857, 959, 1155, 1517, 2075
Bst6I CTCTTC 3 cut(s) 1631, 2181, 2430
BstBAI YACGTR 1 cut(s) 1280
BstBI TTCGAA 2 cut(s) 1502, 2559
BstC8I GCNNGC 5 cut(s) 537, 906, 1071, 1094, 2498
BstDEI CTNAG 9 cut(s) 98, 585, 1115, 1257, 1473, 1694, 2201, 2333, 2471
BstDSI CCRYGG 2 cut(s) 1162, 2356
BstENI CCTNNNNNAGG 1 cut(s) 1349
BstFNI CGCG 1 cut(s) 1149
BstH2I RGCGCY 1 cut(s) 819
BstHHI GCGC 4 cut(s) 818, 1151, 1742, 2167
BstKTI GATC 9 cut(s) 446, 529, 628, 1317, 1619, 1758, 2265, 2304, 2487
BstMAI GTCTC 4 cut(s) 523, 663, 769, 1446
BstMBI GATC 9 cut(s) 443, 526, 625, 1314, 1616, 1755, 2262, 2301, 2484
BstMCI CGRYCG 2 cut(s) 628, 1619
BstMWI GCNNNNNNNGC 6 cut(s) 209, 905, 1055, 1157, 1181, 1190
BstNI CCWGG 2 cut(s) 461, 1703
BstNSI RCATGY 4 cut(s) 908, 1096, 1921, 2624
BstSCI CCNGG 4 cut(s) 81, 459, 1590, 1701
BstSFI CTRYAG 2 cut(s) 1675, 1791
BstUI CGCG 1 cut(s) 1149
BstV1I GCAGC 4 cut(s) 423, 1406, 2301, 2577
BstV2I GAAGAC 5 cut(s) 162, 222, 741, 2027, 2607
BstX2I RGATCY 1 cut(s) 2301
BstYI RGATCY 1 cut(s) 2301
Bsu36I CCTNAGG 1 cut(s) 1257
BsuI GTATCC 1 cut(s) 658
BsuRI GGCC 3 cut(s) 305, 459, 839
BtgI CCRYGG 2 cut(s) 1162, 2356
BtsI GCAGTG 2 cut(s) 128, 1426
BtsIMutI CAGTG 7 cut(s) 128, 862, 987, 1151, 1426, 1485, 2487
BveI ACCTGC 1 cut(s) 157
Cac8I GCNNGC 5 cut(s) 537, 906, 1071, 1094, 2498
CaiI CAGNNNCTG 1 cut(s) 11
CfoI GCGC 4 cut(s) 818, 1151, 1742, 2167
Cfr10I RCCGGY 1 cut(s) 812
Cfr13I GGNCC 3 cut(s) 304, 1166, 1342
CseI GACGC 1 cut(s) 786
Csp6I GTAC 6 cut(s) 47, 259, 393, 1513, 1768, 1921
CspCI CAANNNNNGTGG 4 cut(s) 486, 521, 838, 873
CviQI GTAC 6 cut(s) 47, 259, 393, 1513, 1768, 1921
DdeI CTNAG 9 cut(s) 98, 585, 1115, 1257, 1473, 1694, 2201, 2333, 2471
DpnI GATC 9 cut(s) 445, 528, 627, 1316, 1618, 1757, 2264, 2303, 2486
DpnII GATC 9 cut(s) 443, 526, 625, 1314, 1616, 1755, 2262, 2301, 2484
DraI TTTAAA 2 cut(s) 132, 1687
EaeI YGGCCR 1 cut(s) 457
Eam1104I CTCTTC 3 cut(s) 1631, 2181, 2430
EarI CTCTTC 3 cut(s) 1631, 2181, 2430
EciI GGCGGA 1 cut(s) 2008
Eco130I CCWWGG 2 cut(s) 69, 2356
Eco147I AGGCCT 1 cut(s) 839
Eco24I GRGCYC 1 cut(s) 1555
Eco31I GGTCTC 1 cut(s) 1446
Eco47I GGWCC 2 cut(s) 1166, 1342
Eco57I CTGAAG 6 cut(s) 131, 234, 452, 1507, 1529, 2538
Eco81I CCTNAGG 1 cut(s) 1257
Eco88I CYCGRG 2 cut(s) 1548, 2399
EcoNI CCTNNNNNAGG 1 cut(s) 1349
EcoO109I RGGNCCY 1 cut(s) 1342
EcoRI GAATTC 2 cut(s) 2237, 2417
EcoRII CCWGG 2 cut(s) 459, 1701
EcoT14I CCWWGG 2 cut(s) 69, 2356
EcoT38I GRGCYC 1 cut(s) 1555
ErhI CCWWGG 2 cut(s) 69, 2356
Esp3I CGTCTC 1 cut(s) 769
FblI GTMKAC 1 cut(s) 1063
Fnu4HI GCNGC 6 cut(s) 95, 437, 440, 1395, 2315, 2591
FriOI GRGCYC 1 cut(s) 1555
Fsp4HI GCNGC 6 cut(s) 95, 437, 440, 1395, 2315, 2591
FspBI CTAG 3 cut(s) 1070, 1190, 2408
GlaI GCGC 4 cut(s) 817, 1150, 1741, 2166
GluI GCNGC 6 cut(s) 95, 437, 440, 1395, 2315, 2591
GsuI CTGGAG 2 cut(s) 503, 1955
HaeII RGCGCY 1 cut(s) 819
HaeIII GGCC 3 cut(s) 305, 459, 839
HapII CCGG 5 cut(s) 83, 813, 976, 1575, 1592
HgaI GACGC 1 cut(s) 786
HhaI GCGC 4 cut(s) 818, 1151, 1742, 2167
Hin6I GCGC 4 cut(s) 816, 1149, 1740, 2165
HinP1I GCGC 4 cut(s) 816, 1149, 1740, 2165
HincII GTYRAC 1 cut(s) 1602
HindII GTYRAC 1 cut(s) 1602
HindIII AAGCTT 3 cut(s) 1900, 2059, 2468
HpaII CCGG 5 cut(s) 83, 813, 976, 1575, 1592
HphI GGTGA 3 cut(s) 65, 410, 1432
Hpy166II GTNNAC 4 cut(s) 1064, 1283, 1602, 2480
Hpy8I GTNNAC 4 cut(s) 1064, 1283, 1602, 2480
HpyAV CCTTC 9 cut(s) 395, 416, 764, 850, 932, 1104, 1301, 1326, 2555
HpyCH4III ACNGT 7 cut(s) 657, 827, 857, 959, 1155, 1517, 2075
HpyCH4IV ACGT 5 cut(s) 357, 1279, 1681, 1923, 1992
HpyF10VI GCNNNNNNNGC 6 cut(s) 209, 905, 1055, 1157, 1181, 1190
HpyF3I CTNAG 9 cut(s) 98, 585, 1115, 1257, 1473, 1694, 2201, 2333, 2471
HpySE526I ACGT 5 cut(s) 357, 1279, 1681, 1923, 1992
HspAI GCGC 4 cut(s) 816, 1149, 1740, 2165
Kzo9I GATC 9 cut(s) 443, 526, 625, 1314, 1616, 1755, 2262, 2301, 2484
LmnI GCTCC 3 cut(s) 807, 1626, 2517
Lsp1109I GCAGC 4 cut(s) 423, 1406, 2301, 2577
LweI GCATC 9 cut(s) 108, 127, 133, 199, 901, 2206, 2278, 2499, 2556
MaeI CTAG 3 cut(s) 1070, 1190, 2408
MaeII ACGT 5 cut(s) 357, 1279, 1681, 1923, 1992
MaeIII GTNAC 2 cut(s) 308, 2502
MalI GATC 9 cut(s) 445, 528, 627, 1316, 1618, 1757, 2264, 2303, 2486
MbiI CCGCTC 1 cut(s) 2205
MboI GATC 9 cut(s) 443, 526, 625, 1314, 1616, 1755, 2262, 2301, 2484
MfeI CAATTG 1 cut(s) 1019
MflI RGATCY 1 cut(s) 2301
MhlI GDGCHC 3 cut(s) 756, 812, 1555
MlsI TGGCCA 1 cut(s) 459
MluNI TGGCCA 1 cut(s) 459
MlyI GAGTC 5 cut(s) 257, 418, 1607, 1707, 1831
MmeI TCCRAC 6 cut(s) 85, 723, 941, 1851, 1987, 2421
Mox20I TGGCCA 1 cut(s) 459
MroXI GAANNNNTTC 1 cut(s) 983
MscI TGGCCA 1 cut(s) 459
MslI CAYNNNNRTG 5 cut(s) 417, 677, 909, 1079, 1534
Msp20I TGGCCA 1 cut(s) 459
MspI CCGG 5 cut(s) 83, 813, 976, 1575, 1592
MspR9I CCNGG 4 cut(s) 83, 461, 1592, 1703
MunI CAATTG 1 cut(s) 1019
Mva1269I GAATGC 2 cut(s) 983, 1177
MvaI CCWGG 2 cut(s) 461, 1703
MvnI CGCG 1 cut(s) 1149
MwoI GCNNNNNNNGC 6 cut(s) 209, 905, 1055, 1157, 1181, 1190
NciI CCSGG 2 cut(s) 83, 1592
NcoI CCATGG 1 cut(s) 2356
NdeII GATC 9 cut(s) 443, 526, 625, 1314, 1616, 1755, 2262, 2301, 2484
NheI GCTAGC 1 cut(s) 1069
NlaIV GGNNCC 1 cut(s) 1803
NspI RCATGY 4 cut(s) 908, 1096, 1921, 2624
NspV TTCGAA 2 cut(s) 1502, 2559
PaeI GCATGC 2 cut(s) 908, 1096
PaeR7I CTCGAG 2 cut(s) 1548, 2399
PaqCI CACCTGC 1 cut(s) 157
PceI AGGCCT 1 cut(s) 839
PciI ACATGT 1 cut(s) 1917
PctI GAATGC 2 cut(s) 983, 1177
PdmI GAANNNNTTC 1 cut(s) 983
PfeI GAWTC 5 cut(s) 348, 1504, 2090, 2426, 2577
PflMI CCANNNNNTGG 3 cut(s) 1937, 1978, 2551
PfoI TCCNGGA 1 cut(s) 1701
PkrI GCNGC 6 cut(s) 96, 438, 441, 1396, 2316, 2592
Ple19I CGATCG 2 cut(s) 628, 1619
PleI GAGTC 5 cut(s) 256, 417, 1606, 1706, 1831
PpsI GAGTC 5 cut(s) 256, 417, 1606, 1706, 1831
Ppu21I YACGTR 1 cut(s) 1280
PpuMI RGGWCCY 1 cut(s) 1342
PscI ACATGT 1 cut(s) 1917
PsiI TTATAA 1 cut(s) 689
Psp1406I AACGTT 1 cut(s) 1992
Psp5II RGGWCCY 1 cut(s) 1342
Psp6I CCWGG 2 cut(s) 459, 1701
PspGI CCWGG 2 cut(s) 459, 1701
PspN4I GGNNCC 1 cut(s) 1803
PspPI GGNCC 3 cut(s) 304, 1166, 1342
PspPPI RGGWCCY 1 cut(s) 1342
PspXI VCTCGAGB 1 cut(s) 2399
PstI CTGCAG 1 cut(s) 1679
PstNI CAGNNNCTG 1 cut(s) 11
PsuI RGATCY 1 cut(s) 2301
PvuI CGATCG 2 cut(s) 628, 1619
RsaI GTAC 6 cut(s) 48, 260, 394, 1514, 1769, 1922
RsaNI GTAC 6 cut(s) 47, 259, 393, 1513, 1768, 1921
RseI CAYNNNNRTG 5 cut(s) 417, 677, 909, 1079, 1534
SatI GCNGC 6 cut(s) 95, 437, 440, 1395, 2315, 2591
Sau3AI GATC 9 cut(s) 443, 526, 625, 1314, 1616, 1755, 2262, 2301, 2484
Sau96I GGNCC 3 cut(s) 304, 1166, 1342
ScaI AGTACT 2 cut(s) 394, 1769
SchI GAGTC 5 cut(s) 257, 418, 1607, 1707, 1831
ScrFI CCNGG 4 cut(s) 83, 461, 1592, 1703
SduI GDGCHC 3 cut(s) 756, 812, 1555
SfaNI GCATC 9 cut(s) 108, 127, 133, 199, 901, 2206, 2278, 2499, 2556
SfcI CTRYAG 2 cut(s) 1675, 1791
Sfr274I CTCGAG 2 cut(s) 1548, 2399
SfuI TTCGAA 2 cut(s) 1502, 2559
SgrAI CRCCGGYG 1 cut(s) 812
SinI GGWCC 2 cut(s) 1166, 1342
SlaI CTCGAG 2 cut(s) 1548, 2399
SmiMI CAYNNNNRTG 5 cut(s) 417, 677, 909, 1079, 1534
SmlI CTYRAG 5 cut(s) 299, 1548, 2002, 2306, 2399
SmoI CTYRAG 5 cut(s) 299, 1548, 2002, 2306, 2399
SphI GCATGC 2 cut(s) 908, 1096
SseBI AGGCCT 1 cut(s) 839
SsiI CCGC 9 cut(s) 94, 115, 172, 340, 439, 1067, 1147, 2019, 2205
SspI AATATT 1 cut(s) 1864
SspMI CTAG 3 cut(s) 1070, 1190, 2408
StuI AGGCCT 1 cut(s) 839
StyD4I CCNGG 4 cut(s) 81, 459, 1590, 1701
StyI CCWWGG 2 cut(s) 69, 2356
TaaI ACNGT 7 cut(s) 657, 827, 857, 959, 1155, 1517, 2075
TaiI ACGT 5 cut(s) 360, 1282, 1684, 1926, 1995
TaqI TCGA 6 cut(s) 624, 1502, 1549, 1639, 2400, 2559
TatI WGTACW 5 cut(s) 46, 258, 392, 1512, 1767
TauI GCSGC 2 cut(s) 97, 442
TfiI GAWTC 5 cut(s) 348, 1504, 2090, 2426, 2577
TscAI CASTG 7 cut(s) 128, 862, 994, 1158, 1433, 1485, 2494
TseI GCWGC 4 cut(s) 436, 1394, 2314, 2590
TspDTI ATGAA 9 cut(s) 435, 945, 1487, 1575, 1733, 1910, 1916, 2250, 2569
TspGWI ACGGA 1 cut(s) 2521
TspRI CASTG 7 cut(s) 128, 862, 994, 1158, 1433, 1485, 2494
Van91I CCANNNNNTGG 3 cut(s) 1937, 1978, 2551
VpaK11BI GGWCC 2 cut(s) 1166, 1342
XagI CCTNNNNNAGG 1 cut(s) 1349
XapI RAATTY 7 cut(s) 1226, 1484, 1656, 2082, 2237, 2372, 2417
XbaI TCTAGA 1 cut(s) 2407
XceI RCATGY 4 cut(s) 908, 1096, 1921, 2624
XcmI CCANNNNNNNNNTGG 1 cut(s) 1081
XhoI CTCGAG 2 cut(s) 1548, 2399
XmiI GTMKAC 1 cut(s) 1063
XmnI GAANNNNTTC 1 cut(s) 983
XspI CTAG 3 cut(s) 1070, 1190, 2408
ZrmI AGTACT 2 cut(s) 394, 1769
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.