Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0002006

Physical Location & Seq

Genomic Coordinates Forward (+)

1790208 .. 1796146

Gene Structure 5939 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1029100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2988 bp

ATGATTAAACAATTACACACTGTATGTATCTGTATGCAAACCACAGCGGAATGGCTCAAGGTTCCTGTCCAGGGAATTAAAAGGTTTGGAGAAGAAATGAGGCCTGTAAAGTTGACAACAAAAGTTTTTGATATCCTCATGCAATATTCAAGAGGGAAATCCGCTTTGGTTTTCTGTTCAACTAGAAAAGGTGCACAAGAAGCAGCACAGAGGCTCACGCAGACAGTCATGGCCTTTGGCCACTCAAATCCATTCATTAAAAGCAGAGAACAGCAAGAGAGGCTAAGGGAAGCTTCATTTTCATGTGGTGACAAACAAATGCAATCTTATATTCCTTACGGTGTTGGTTATCACAACGGTGGCCTTTCCATGAAGGATCGTAACCTCATTGAAAGTCTTTTTCTTAAGGGTGATATTCAAGTTTTGTGCACAACAAATACCCTTGCTCATGGAATCAACCTACCAGCACATACAGTAGTTATAAAATCAACACAGCACTTCAACAAAGAAAAAGGTCTCTACATGGAATATGACCGGTCTACAATACTACAGATGTGTGGGAGGGCTGGCCGACCACCATTTGATGATACAGGAATGGTTGTAATCATGACAAGAAAAGACACGGTTCACTTGTACGAAAATCTATTGAATGGATGCGAGATGGTGGAATCACAATTGCTTTCATGTTTGACGGAGCATTTAACCGCTGAGATAGTTCAATTGACTGTCTCTAATATTCCAAGGGCAATTGAATGGATGAAGTGCTCTTTCTTGTACGTGAGAATGAAGAAGAACCCTGTGCACTATTCAGTTAGAAAAGTGATTTCTAAAGACCGTATAGAAAAGCATATGCAAGAGGTCTGTCTGCAGAAAGTTAATGAGTTGTCACAGCATCAGATGATATGGACGGATAAGGATGGTTTTCTTTTGAAACCACTAGAACCTGGGAGGCTGATGACAAAATACTACTTGAAATTTGACACAATGAAACACATTATGCAGACACCTGTAAACTGCAGTTTGGAAGATGCTCTTCATGTTATATGTCGAGCTGAGGAAATTTCATGGATACAACTCAGACGCAGTGAGAAGAAGCTTTTAAATGACATAAATGCTGATAAAGATGGCCGACTTCGCTTTCATGCCCTTGGTGACAAAGGGAGGCGACAAAAGCGTATTCAGACCAGAGAAGAAAAAACATTTGTTCTGGCTAATGACTGCTTGACCGGTGATCCTTCGGTTCATGATCTATCCCTTACTCAGGATATGAACTCTATATGCTCAAATGGCTGTAGAATTGCAAGGTGTATGAAAGAATTTTTTATTTACCAAAAGAACTATAAGGGAGCCTTGAACTCGATGCTTCTGGCAAAGTCGTTATATCAAAAACTCTGGGATGACAGCCCATACCTGCTGAAACAATTACCCGGGATTGGAATGGTGACTGCAAAGGCACTACATTCAATGGGAGTCAGATCGTTCGACACACTGGCTGAGGCTGATCCTAGGAGAATAGAGATAGTCACTGGTCGAAAATACCCATTTGGAAACCATTTAAAAGAGTCTCTACATTCACTACCTCCAAAAGTTGAGATGAAGTTCGAAGAAAATGAGTGTCAGAGGCAAGGGAAACTGAAGCTCGTGGTAACATTGACTAGGCTATCACCGACACTCCAACCAACCAAACGACATTATGCTGATATGATTATTGGTTCAGAAGAGGATAACCTGATTGTCTTTCATGAGAAGATAAGAGTGGAGGAATTCTCCAGCCCCTATAGCGCAACAATTCTTCTGTCGAATCCCCAACAAGGAAAGCTCACTGTAAAGGCTGATCTTGTTTTTGAAGAATACATCGGCATTGATCTCTCCCAGAAACTTGTATTAATGAAGGAAGGCAATAACAAGCGGGGAAAAAAGCCGCCTTCTTGTCCTCCACCTAAGGAAGTATGTGTCATAGAGGATGATGCTGAAACTATACGTCAGGCTCCAACGAAAGAGCATCCCAAATCGACCAAATCCAAAAGGGTAGATGGTTCCATGCCGAGTTTCAACCTCCTGGATGATGAGTTCGAAGAAGTTTTTAAATCCTTCCCTCCTAATCACCAACAAATTTCACCTCGAATTTCTGAAGACGAGGTTGCTGCCAAAGATGAAGAGGAGGATTGCAAAATCATCACACACCAAACGGTATTTGATCACATACGTGAAAAGGCAAAGAACTTCCCCGTCTTGGTGGCATCAAATGGTGCGCATTCTGCATCCACAGAGCCCTTGGTTTTGACAAGAAAGCGTGCTCGTGAGAAGCAGATTGAACCCCATAGAGAATTCGAGGTTCTGGAAGAGTTAGAATGGAATAAAATTCCAAGGCGGACTGCGGTGAATCCATCTTCAAAACCCAAAGAGGCAGAACAAAATCAACATAACACGAACAATCTCCCTACTCCCAGAAGCTCGGCTACCTTGAACCCGATGGATGACAGAGAAGCTGGTCTTCCTCTTGAACCCGAAGATGGAACTTTCAAAACTGCAAGAGATGAAACAATATTTGAGCACATTCGAACAAAAGCTAAGAATTTTCCGGTGATCAATAAACCGAAAGTGGTGGATTCTGGTTATCCAACCATGAAAGAACATCCTTCCAAGAGTCGCCTTGAATTTAGTATCGATACGACTGGCACGCTGAAAGGAACAGCAAAGTCAAACAAAGTTCCGAGAGACACTGCATTCGACTCAGATTCGGGAACCAGAAAAGCATCAAATGTGTCTCCTAGAGGTTTAGGCGACGGTGCTGCAAAAATGCTGTCTTTTGATATCTCCATGTTGAAGAATACGAAGAAATCACCGGAACCAGGCAGTGCTGGCAAGAGGAAACATGAGCTTTCTCCGCTTGTACCACAAAGGCAGTGGTGCTCGTTGACATCTGCTGGCAAAACCAGGGAAGCAGACACATTTCTTGGATTTGAGAGTATCTTTTCGTTCCTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000280 GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005730 GO:0005737 GO:0006139 GO:0006259 GO:0006310 GO:0006725 GO:0006807 GO:0006996 GO:0007049 GO:0007127 GO:0007131 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010501 GO:0016043 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022402 GO:0022414 GO:0031974 GO:0031981 GO:0034641 GO:0035825 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0048285 GO:0051321 GO:0061982 GO:0070013 GO:0070035 GO:0071704 GO:0071840 GO:0090304 GO:0140013 GO:0140098 GO:1901360 GO:1903046

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD HHH_5 Helicase_C PPR Sec63

Protein Analysis

996

Amino Acids

113.1

Weight (kDa)

8.99

Isoelectric Point (pI)

44.13

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Helicase_C	PF00271	110 - 191	7.8e-10	Helicase conserved C-terminal domain
WHD_SNRNP200	PF23445	223 - 302	6.2e-14	Winged Helix-turn-helix domain
Sec63	PF02889	312 - 626	1.3e-46	Sec63 Brl domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002006)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G27730 AT3G27730 AT3G27730 AT3G27730 AT3G27730 AT3G27730 AT3G27730
fragaria_vesca	FvH4_6g51100
malus_domestica	MD09G1029000.v1.1 MD09G1029100.v1.1
prunus_persica	Prupe.3G288900_v2.0.a1
pyrus_communis	pycom111g02300 pycom111g02310
rosa_chinensis	RchiOBHm_Chr2g0089321 RchiOBHm_Chr2g0172661 RchiOBHm_Chr2g0172671
rosa_laevigata	RLG00000022075 RLG00000022122 RLG00000022126
rosa_multiflora	Rmu_sc0002053.1_g000008
rosa_roxburghii	Rroxscaffold_2G00079680 Rroxscaffold_2G00080200 Rroxscaffold_2G00080420
rosa_rugosa	Rorug01G0489000 Rorug01G0489100 Rorug02G0246000 Rorug02G0560500.1
rosa_samantha	Rh2AG637300 Rh2AG637400 Rh2AG637500 Rh2AG637900 Rh2BG650700 Rh2BG650800 Rh2BG650900 Rh2BG651500 Rh2CG045200 Rh2CG045300 Rh2CG610500 Rh2CG617200 Rh2CG617600 Rh2DG658400 Rh2DG664700 Rh2DG665400
rosa_wichuraiana	Rw2G052240 Rw2G052720

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	482
Acc16I	TGCGCA	1 cut(s)	2253
Acc36I	ACCTGC	1 cut(s)	1419
AccI	GTMKAC	1 cut(s)	539
AciI	CCGC	8 cut(s)	47, 162, 705, 1909, 1922, 2371, 2378, 2888
AclWI	GGATC	3 cut(s)	384, 1226, 1496
AcoI	YGGCCR	3 cut(s)	238, 568, 1126
AcuI	CTGAAG	2 cut(s)	1655, 2151
AfaI	GTAC	3 cut(s)	635, 776, 2895
AfiI	CCNNNNNNNGG	7 cut(s)	71, 1261, 1433, 1811, 2233, 2513, 2852
AflII	CTTAAG	1 cut(s)	404
AgeI	ACCGGT	2 cut(s)	534, 1226
AjnI	CCWGG	5 cut(s)	69, 943, 2058, 2851, 2936
AjuI	GAANNNNNNNTTGG	2 cut(s)	149, 181
AleI	CACNNNNGTG	2 cut(s)	357, 2205
AloI	GAACNNNNNNTCC	2 cut(s)	2054, 2086
AluBI	AGCT	9 cut(s)	293, 1052, 1096, 1639, 1819, 2454, 2489, 2570, 2881
AluI	AGCT	9 cut(s)	293, 1052, 1096, 1639, 1819, 2454, 2489, 2570, 2881
Alw21I	GWGCWC	7 cut(s)	196, 431, 767, 804, 2299, 2556, 2915
Alw26I	GTCTC	5 cut(s)	521, 733, 1569, 2712, 2772
Alw44I	GTGCAC	3 cut(s)	192, 427, 800
AlwI	GGATC	3 cut(s)	384, 1226, 1496
Ama87I	CYCGRG	1 cut(s)	1427
AoxI	GGCC	6 cut(s)	101, 231, 238, 361, 568, 1126
ApaLI	GTGCAC	3 cut(s)	192, 427, 800
ApeKI	GCWGC	3 cut(s)	203, 2144, 2792
ArsI	GACNNNNNNTTYG	4 cut(s)	2711, 2743, 2789, 2821
AseI	ATTAAT	1 cut(s)	1886
AsiGI	ACCGGT	2 cut(s)	534, 1226
AspA2I	CCTAGG	1 cut(s)	1505
AspLEI	GCGC	2 cut(s)	1784, 2254
AsuC2I	CCSGG	2 cut(s)	1428, 1429
AsuII	TTCGAA	3 cut(s)	1602, 2073, 2560
AvaI	CYCGRG	1 cut(s)	1427
AvrII	CCTAGG	1 cut(s)	1505
AxyI	CCTNAGG	1 cut(s)	1941
BaeGI	GKGCMC	3 cut(s)	196, 431, 804
BalI	TGGCCA	1 cut(s)	240
BanII	GRGCYC	1 cut(s)	2274
BarI	GAAGNNNNNNTAC	2 cut(s)	2443, 2475
BauI	CACGAG	2 cut(s)	1640, 2298
BbsI	GAAGAC	2 cut(s)	2139, 2486
Bbv12I	GWGCWC	7 cut(s)	196, 431, 767, 804, 2299, 2556, 2915
BbvCI	CCTCAGC	2 cut(s)	1053, 1494
BbvI	GCAGC	3 cut(s)	215, 2131, 2779
BccI	CCATC	7 cut(s)	655, 911, 1118, 2027, 2395, 2467, 2507
BcgI	CGANNNNNNTGC	4 cut(s)	2126, 2160, 2774, 2808
BciT130I	CCWGG	5 cut(s)	71, 945, 2060, 2853, 2938
BciVI	GTATCC	1 cut(s)	1062
BclI	TGATCA	2 cut(s)	2197, 2586
BcnI	CCSGG	2 cut(s)	1428, 1429
BcoDI	GTCTC	5 cut(s)	521, 733, 1569, 2712, 2772
BfaI	CTAG	5 cut(s)	183, 938, 1506, 1656, 2772
BfmI	CTRYAG	5 cut(s)	548, 866, 1015, 1291, 1777
BfrI	CTTAAG	1 cut(s)	404
BfuAI	ACCTGC	1 cut(s)	1419
BfuI	GTATCC	1 cut(s)	1062
BisI	GCNGC	4 cut(s)	204, 1922, 2145, 2793
BlnI	CCTAGG	1 cut(s)	1505
BlsI	GCNGC	4 cut(s)	205, 1923, 2146, 2794
Bme1390I	CCNGG	7 cut(s)	71, 945, 1428, 1429, 2060, 2853, 2938
BmeT110I	CYCGRG	1 cut(s)	1427
BmiI	GGNNCC	6 cut(s)	63, 1348, 1989, 2038, 2746, 2850
BmrFI	CCNGG	7 cut(s)	71, 945, 1428, 1429, 2060, 2853, 2938
BmsI	GCATC	9 cut(s)	644, 901, 1018, 1350, 1957, 2011, 2249, 2270, 2765
BpiI	GAAGAC	2 cut(s)	2139, 2486
BplI	GAGNNNNNCTC	2 cut(s)	1751, 1783
BpmI	CTGGAG	1 cut(s)	1753
Bpu10I	CCTNAGC	3 cut(s)	284, 1053, 1494
Bpu14I	TTCGAA	3 cut(s)	1602, 2073, 2560
BpuEI	CTTGAG	1 cut(s)	41
BpuMI	CCSGG	2 cut(s)	1428, 1429
Bsa29I	ATCGAT	1 cut(s)	2667
BsaAI	YACGTR	2 cut(s)	778, 2207
BsaI	GGTCTC	1 cut(s)	521
BsaJI	CCNNGG	9 cut(s)	70, 740, 944, 1147, 1427, 1505, 2274, 2366, 2937
BsaWI	WCCGGW	4 cut(s)	534, 1226, 2581, 2845
BsaXI	ACNNNNNCTCC	2 cut(s)	1656, 1686
Bsc4I	CCNNNNNNNGG	7 cut(s)	71, 1261, 1433, 1811, 2233, 2513, 2852
Bse118I	RCCGGY	2 cut(s)	534, 1226
Bse1I	ACTGG	3 cut(s)	1494, 1531, 2680
Bse21I	CCTNAGG	1 cut(s)	1941
BseBI	CCWGG	5 cut(s)	71, 945, 2060, 2853, 2938
BseCI	ATCGAT	1 cut(s)	2667
BseDI	CCNNGG	9 cut(s)	70, 740, 944, 1147, 1427, 1505, 2274, 2366, 2937
BseLI	CCNNNNNNNGG	7 cut(s)	71, 1261, 1433, 1811, 2233, 2513, 2852
BseMII	CTCAG	6 cut(s)	699, 1044, 1090, 1274, 1485, 2748
BseNI	ACTGG	3 cut(s)	1494, 1531, 2680
BseRI	GAGGAG	1 cut(s)	2174
BseSI	GKGCMC	3 cut(s)	196, 431, 804
BseXI	GCAGC	3 cut(s)	215, 2131, 2779
BshFI	GGCC	6 cut(s)	103, 233, 240, 363, 570, 1128
BshTI	ACCGGT	2 cut(s)	534, 1226
BshVI	ATCGAT	1 cut(s)	2667
BsiHKAI	GWGCWC	7 cut(s)	196, 431, 767, 804, 2299, 2556, 2915
BsiHKCI	CYCGRG	1 cut(s)	1427
BsiSI	CCGG	5 cut(s)	535, 1227, 1428, 2582, 2846
BslI	CCNNNNNNNGG	7 cut(s)	71, 1261, 1433, 1811, 2233, 2513, 2852
BsmAI	GTCTC	5 cut(s)	521, 733, 1569, 2712, 2772
BsmI	GAATGC	2 cut(s)	2254, 2726
BsnI	GGCC	6 cut(s)	103, 233, 240, 363, 570, 1128
Bso31I	GGTCTC	1 cut(s)	521
BsoBI	CYCGRG	1 cut(s)	1427
Bsp119I	TTCGAA	3 cut(s)	1602, 2073, 2560
Bsp1286I	GDGCHC	8 cut(s)	196, 431, 767, 804, 2274, 2299, 2556, 2915
Bsp143I	GATC	9 cut(s)	376, 1231, 1246, 1475, 1501, 1834, 1864, 2197, 2586
BspACI	CCGC	8 cut(s)	47, 162, 705, 1909, 1922, 2371, 2378, 2888
BspANI	GGCC	6 cut(s)	103, 233, 240, 363, 570, 1128
BspCNI	CTCAG	6 cut(s)	700, 1045, 1089, 1273, 1486, 2747
BspDI	ATCGAT	1 cut(s)	2667
BspHI	TCATGA	3 cut(s)	606, 1243, 1741
BspLI	GGNNCC	6 cut(s)	63, 1348, 1989, 2038, 2746, 2850
BspMAI	CTGCAG	2 cut(s)	870, 1019
BspMI	ACCTGC	1 cut(s)	1419
BspPI	GGATC	3 cut(s)	384, 1226, 1496
BspQI	GCTCTTC	1 cut(s)	1038
BspT104I	TTCGAA	3 cut(s)	1602, 2073, 2560
BspTI	CTTAAG	1 cut(s)	404
BspTNI	GGTCTC	1 cut(s)	521
BsrFI	RCCGGY	2 cut(s)	534, 1226
BsrI	ACTGG	3 cut(s)	1494, 1531, 2680
BssAI	RCCGGY	2 cut(s)	534, 1226
BssECI	CCNNGG	9 cut(s)	70, 740, 944, 1147, 1427, 1505, 2274, 2366, 2937
BssMI	GATC	9 cut(s)	376, 1231, 1246, 1475, 1501, 1834, 1864, 2197, 2586
BssSI	CACGAG	2 cut(s)	1640, 2298
BssT1I	CCWWGG	5 cut(s)	740, 1147, 1505, 2274, 2366
Bst2BI	CACGAG	2 cut(s)	1640, 2298
Bst2UI	CCWGG	5 cut(s)	71, 945, 2060, 2853, 2938
Bst6I	CTCTTC	4 cut(s)	1038, 1713, 2151, 2337
BstAFI	CTTAAG	1 cut(s)	404
BstBAI	YACGTR	2 cut(s)	778, 2207
BstBI	TTCGAA	3 cut(s)	1602, 2073, 2560
BstC8I	GCNNGC	5 cut(s)	568, 2295, 2681, 2863, 2929
BstDEI	CTNAG	9 cut(s)	284, 708, 1053, 1076, 1260, 1494, 1941, 2571, 2734
BstENI	CCTNNNNNAGG	1 cut(s)	1259
BstHHI	GCGC	2 cut(s)	1784, 2254
BstKTI	GATC	9 cut(s)	379, 1234, 1249, 1478, 1504, 1837, 1867, 2200, 2589
BstMAI	GTCTC	5 cut(s)	521, 733, 1569, 2712, 2772
BstMBI	GATC	9 cut(s)	376, 1231, 1246, 1475, 1501, 1834, 1864, 2197, 2586
BstMWI	GCNNNNNNNGC	9 cut(s)	200, 280, 1134, 1171, 1287, 1779, 2258, 2862, 2887
BstNI	CCWGG	5 cut(s)	71, 945, 2060, 2853, 2938
BstSCI	CCNGG	7 cut(s)	69, 943, 1426, 1427, 2058, 2851, 2936
BstSFI	CTRYAG	5 cut(s)	548, 866, 1015, 1291, 1777
BstSLI	GKGCMC	3 cut(s)	196, 431, 804
BstV1I	GCAGC	3 cut(s)	215, 2131, 2779
BstV2I	GAAGAC	2 cut(s)	2139, 2486
Bsu15I	ATCGAT	1 cut(s)	2667
Bsu36I	CCTNAGG	1 cut(s)	1941
BsuI	GTATCC	1 cut(s)	1062
BsuRI	GGCC	6 cut(s)	103, 233, 240, 363, 570, 1128
BsuTUI	ATCGAT	1 cut(s)	2667
BtsI	GCAGTG	4 cut(s)	1090, 2721, 2863, 2912
BtsIMutI	CAGTG	8 cut(s)	18, 1090, 1487, 1524, 1821, 2721, 2863, 2912
BveI	ACCTGC	1 cut(s)	1419
Cac8I	GCNNGC	5 cut(s)	568, 2295, 2681, 2863, 2929
CciI	TCATGA	3 cut(s)	606, 1243, 1741
CfoI	GCGC	2 cut(s)	1784, 2254
Cfr10I	RCCGGY	2 cut(s)	534, 1226
Cfr9I	CCCGGG	1 cut(s)	1427
ClaI	ATCGAT	1 cut(s)	2667
CseI	GACGC	1 cut(s)	1089
Csp6I	GTAC	3 cut(s)	634, 775, 2894
CspAI	ACCGGT	2 cut(s)	534, 1226
CspCI	CAANNNNNGTGG	2 cut(s)	2888, 2923
CviQI	GTAC	3 cut(s)	634, 775, 2894
DdeI	CTNAG	9 cut(s)	284, 708, 1053, 1076, 1260, 1494, 1941, 2571, 2734
DpnI	GATC	9 cut(s)	378, 1233, 1248, 1477, 1503, 1836, 1866, 2199, 2588
DpnII	GATC	9 cut(s)	376, 1231, 1246, 1475, 1501, 1834, 1864, 2197, 2586
DraI	TTTAAA	3 cut(s)	1101, 1557, 2086
EaeI	YGGCCR	3 cut(s)	238, 568, 1126
Eam1104I	CTCTTC	4 cut(s)	1038, 1713, 2151, 2337
EarI	CTCTTC	4 cut(s)	1038, 1713, 2151, 2337
EciI	GGCGGA	1 cut(s)	2386
Eco130I	CCWWGG	5 cut(s)	740, 1147, 1505, 2274, 2366
Eco147I	AGGCCT	1 cut(s)	103
Eco24I	GRGCYC	1 cut(s)	2274
Eco31I	GGTCTC	1 cut(s)	521
Eco32I	GATATC	2 cut(s)	133, 2815
Eco57I	CTGAAG	2 cut(s)	1655, 2151
Eco81I	CCTNAGG	1 cut(s)	1941
Eco88I	CYCGRG	1 cut(s)	1427
EcoNI	CCTNNNNNAGG	1 cut(s)	1259
EcoRI	GAATTC	2 cut(s)	1763, 2327
EcoRII	CCWGG	5 cut(s)	69, 943, 2058, 2851, 2936
EcoRV	GATATC	2 cut(s)	133, 2815
EcoT14I	CCWWGG	5 cut(s)	740, 1147, 1505, 2274, 2366
EcoT38I	GRGCYC	1 cut(s)	2274
ErhI	CCWWGG	5 cut(s)	740, 1147, 1505, 2274, 2366
FalI	AAGNNNNNCTT	8 cut(s)	277, 309, 1017, 1049, 1334, 1366, 2478, 2510
FauI	CCCGC	1 cut(s)	1902
FauNDI	CATATG	1 cut(s)	849
FbaI	TGATCA	2 cut(s)	2197, 2586
FblI	GTMKAC	1 cut(s)	539
Fnu4HI	GCNGC	4 cut(s)	204, 1922, 2145, 2793
FriOI	GRGCYC	1 cut(s)	2274
Fsp4HI	GCNGC	4 cut(s)	204, 1922, 2145, 2793
FspAI	RTGCGCAY	1 cut(s)	2253
FspBI	CTAG	5 cut(s)	183, 938, 1506, 1656, 2772
FspI	TGCGCA	1 cut(s)	2253
GlaI	GCGC	2 cut(s)	1783, 2253
GluI	GCNGC	4 cut(s)	204, 1922, 2145, 2793
GsuI	CTGGAG	1 cut(s)	1753
HaeIII	GGCC	6 cut(s)	103, 233, 240, 363, 570, 1128
HapII	CCGG	5 cut(s)	535, 1227, 1428, 2582, 2846
HgaI	GACGC	1 cut(s)	1089
HhaI	GCGC	2 cut(s)	1784, 2254
Hin6I	GCGC	2 cut(s)	1782, 2252
HinP1I	GCGC	2 cut(s)	1782, 2252
HincII	GTYRAC	2 cut(s)	114, 2919
HindII	GTYRAC	2 cut(s)	114, 2919
HindIII	AAGCTT	2 cut(s)	291, 1094
HpaII	CCGG	5 cut(s)	535, 1227, 1428, 2582, 2846
Hpy166II	GTNNAC	8 cut(s)	114, 194, 429, 540, 628, 802, 1012, 2919
Hpy188III	TCNNGA	9 cut(s)	150, 607, 1244, 1262, 1742, 2300, 2339, 2501, 2742
Hpy8I	GTNNAC	8 cut(s)	114, 194, 429, 540, 628, 802, 1012, 2919
Hpy99I	CGWCG	1 cut(s)	2789
HpyAV	CCTTC	7 cut(s)	367, 1245, 1885, 1889, 1935, 2101, 2649
HpyCH4IV	ACGT	3 cut(s)	777, 1981, 2206
HpyF10VI	GCNNNNNNNGC	9 cut(s)	200, 280, 1134, 1171, 1287, 1779, 2258, 2862, 2887
HpyF3I	CTNAG	9 cut(s)	284, 708, 1053, 1076, 1260, 1494, 1941, 2571, 2734
HpySE526I	ACGT	3 cut(s)	777, 1981, 2206
HspAI	GCGC	2 cut(s)	1782, 2252
Ksp22I	TGATCA	2 cut(s)	2197, 2586
Kzo9I	GATC	9 cut(s)	376, 1231, 1246, 1475, 1501, 1834, 1864, 2197, 2586
LguI	GCTCTTC	1 cut(s)	1038
LmnI	GCTCC	3 cut(s)	694, 1346, 1993
Lsp1109I	GCAGC	3 cut(s)	215, 2131, 2779
LweI	GCATC	9 cut(s)	644, 901, 1018, 1350, 1957, 2011, 2249, 2270, 2765
MaeI	CTAG	5 cut(s)	183, 938, 1506, 1656, 2772
MaeII	ACGT	3 cut(s)	777, 1981, 2206
MaeIII	GTNAC	7 cut(s)	308, 380, 885, 1151, 1441, 1522, 1645
MalI	GATC	9 cut(s)	378, 1233, 1248, 1477, 1503, 1836, 1866, 2199, 2588
MboI	GATC	9 cut(s)	376, 1231, 1246, 1475, 1501, 1834, 1864, 2197, 2586
MfeI	CAATTG	3 cut(s)	674, 719, 747
MhlI	GDGCHC	8 cut(s)	196, 431, 767, 804, 2274, 2299, 2556, 2915
MlsI	TGGCCA	1 cut(s)	240
MluNI	TGGCCA	1 cut(s)	240
MlyI	GAGTC	4 cut(s)	1479, 1571, 2656, 2726
MmeI	TCCRAC	3 cut(s)	1699, 2015, 2645
Mox20I	TGGCCA	1 cut(s)	240
MscI	TGGCCA	1 cut(s)	240
MslI	CAYNNNNRTG	3 cut(s)	301, 357, 2205
Msp20I	TGGCCA	1 cut(s)	240
MspA1I	CMGCKG	2 cut(s)	47, 707
MspCI	CTTAAG	1 cut(s)	404
MspI	CCGG	5 cut(s)	535, 1227, 1428, 2582, 2846
MspR9I	CCNGG	7 cut(s)	71, 945, 1428, 1429, 2060, 2853, 2938
MunI	CAATTG	3 cut(s)	674, 719, 747
Mva1269I	GAATGC	2 cut(s)	2254, 2726
MvaI	CCWGG	5 cut(s)	71, 945, 2060, 2853, 2938
MwoI	GCNNNNNNNGC	9 cut(s)	200, 280, 1134, 1171, 1287, 1779, 2258, 2862, 2887
NciI	CCSGG	2 cut(s)	1428, 1429
NdeI	CATATG	1 cut(s)	849
NdeII	GATC	9 cut(s)	376, 1231, 1246, 1475, 1501, 1834, 1864, 2197, 2586
NlaIV	GGNNCC	6 cut(s)	63, 1348, 1989, 2038, 2746, 2850
NmeAIII	GCCGAG	2 cut(s)	2070, 2435
NmuCI	GTSAC	5 cut(s)	308, 885, 1151, 1441, 1522
NsbI	TGCGCA	1 cut(s)	2253
NspV	TTCGAA	3 cut(s)	1602, 2073, 2560
OliI	CACNNNNGTG	2 cut(s)	357, 2205
PagI	TCATGA	3 cut(s)	606, 1243, 1741
PceI	AGGCCT	1 cut(s)	103
PciSI	GCTCTTC	1 cut(s)	1038
PctI	GAATGC	2 cut(s)	2254, 2726
PfeI	GAWTC	6 cut(s)	453, 668, 1801, 2383, 2609, 2738
PfoI	TCCNGGA	1 cut(s)	2058
PinAI	ACCGGT	2 cut(s)	534, 1226
PkrI	GCNGC	4 cut(s)	205, 1923, 2146, 2794
PleI	GAGTC	4 cut(s)	1478, 1570, 2655, 2726
PpsI	GAGTC	4 cut(s)	1478, 1570, 2655, 2726
Ppu21I	YACGTR	2 cut(s)	778, 2207
PshBI	ATTAAT	1 cut(s)	1886
PsiI	TTATAA	1 cut(s)	482
Psp6I	CCWGG	5 cut(s)	69, 943, 2058, 2851, 2936
PspGI	CCWGG	5 cut(s)	69, 943, 2058, 2851, 2936
PspN4I	GGNNCC	6 cut(s)	63, 1348, 1989, 2038, 2746, 2850
PstI	CTGCAG	2 cut(s)	870, 1019
RsaI	GTAC	3 cut(s)	635, 776, 2895
RsaNI	GTAC	3 cut(s)	634, 775, 2894
RseI	CAYNNNNRTG	3 cut(s)	301, 357, 2205
SapI	GCTCTTC	1 cut(s)	1038
SatI	GCNGC	4 cut(s)	204, 1922, 2145, 2793
Sau3AI	GATC	9 cut(s)	376, 1231, 1246, 1475, 1501, 1834, 1864, 2197, 2586
SchI	GAGTC	4 cut(s)	1479, 1571, 2656, 2726
ScrFI	CCNGG	7 cut(s)	71, 945, 1428, 1429, 2060, 2853, 2938
SduI	GDGCHC	8 cut(s)	196, 431, 767, 804, 2274, 2299, 2556, 2915
SfaNI	GCATC	9 cut(s)	644, 901, 1018, 1350, 1957, 2011, 2249, 2270, 2765
SfcI	CTRYAG	5 cut(s)	548, 866, 1015, 1291, 1777
SfuI	TTCGAA	3 cut(s)	1602, 2073, 2560
SmaI	CCCGGG	1 cut(s)	1429
SmiMI	CAYNNNNRTG	3 cut(s)	301, 357, 2205
SmlI	CTYRAG	2 cut(s)	56, 404
SmoI	CTYRAG	2 cut(s)	56, 404
SseBI	AGGCCT	1 cut(s)	103
SsiI	CCGC	8 cut(s)	47, 162, 705, 1909, 1922, 2371, 2378, 2888
SspI	AATATT	3 cut(s)	146, 736, 2547
SspMI	CTAG	5 cut(s)	183, 938, 1506, 1656, 2772
StuI	AGGCCT	1 cut(s)	103
StyD4I	CCNGG	7 cut(s)	69, 943, 1426, 1427, 2058, 2851, 2936
StyI	CCWWGG	5 cut(s)	740, 1147, 1505, 2274, 2366
TaiI	ACGT	3 cut(s)	780, 1984, 2209
TauI	GCSGC	1 cut(s)	1924
TfiI	GAWTC	6 cut(s)	453, 668, 1801, 2383, 2609, 2738
TscAI	CASTG	8 cut(s)	25, 1090, 1494, 1531, 1828, 2728, 2863, 2912
TseFI	GTSAC	5 cut(s)	308, 885, 1151, 1441, 1522
TseI	GCWGC	3 cut(s)	203, 2144, 2792
Tsp45I	GTSAC	5 cut(s)	308, 885, 1151, 1441, 1522
TspGWI	ACGGA	2 cut(s)	707, 923
TspMI	CCCGGG	1 cut(s)	1427
TspRI	CASTG	8 cut(s)	25, 1090, 1494, 1531, 1828, 2728, 2863, 2912
Vha464I	CTTAAG	1 cut(s)	404
VneI	GTGCAC	3 cut(s)	192, 427, 800
VspI	ATTAAT	1 cut(s)	1886
XagI	CCTNNNNNAGG	1 cut(s)	1259
XcmI	CCANNNNNNNNNTGG	2 cut(s)	2272, 2904
XmaI	CCCGGG	1 cut(s)	1427
XmaJI	CCTAGG	1 cut(s)	1505
XmiI	GTMKAC	1 cut(s)	539
XspI	CTAG	5 cut(s)	183, 938, 1506, 1656, 2772

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.