Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0002006

Physical Location & Seq

Genomic Coordinates Forward (+)

80680038 .. 80693949

Gene Structure 13912 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000022075

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4197 bp

ATGACGGAGAGAAATGTGAATGATGAATGCTTGATCGACATTAGAGAAGAGGAAGAGAGTGTTATAGCAGCAACTAATCTGAGTAATCAAATTGTGAGAGAAGACAAAGTCAACGCTGTGCTCTACAATATTAAAGCGAAGCTAATTTCCACAGCTCCATCACAGACTCCTACTTGTATATATAGAATCTCTGACCGTATAAAGAAGCTTCATGAAACGGAGTTCGTCCCCAAATTAGTTTCAATCAGACCACTCCATCATGGCAAGAAGAACCTTCAAGCTATGGAAGCAGTTAAATTTTGTCCTCATCGTCTTCCTTCACTTCGCACAGTCCAGACCTCTCTACTGCGGCCCCGACCTCTTCACCATAATCGACCTCTGTATCGTTCTTTCACTCTTCACGCGCACGCGCTTAGATTAACCCGATCGTTTTTCAGATCGACGGCCGGTACCGGAATTTCATCTTCAATCCAGGAAACGGGTTCAAGCATGGATTCGTATACACTTAAGTCCATGGCAGAATTGCCGGCTCCTTTTCGAGCATGTTTTAATTTTAGGTATTTCAACTCTTTGCAGAGTGAATGCCTTCCTGCTTGTTTCCACTCAGATGTGAACATGGTTGTGTCAGCGCCAACTGGAAGTGGTAAAACAGTCATTTTTGAGCTTTGCATTTTGAGGCTTCTTTCAAGATTCATCTCTGAGGATGGGAGGTTTACACCTACAAATGAAACTCTCAAAACAATCTATATTGCCCCATCAAAGGCTTTAGTTCAGGAGAAGGTTCGTGATTGGACCCAGAAGTTTGGATCTCGGGGCATAGTTTGCTTGGAGCTCACCGGAGACAATGAATCTTGCAACACCAGGAGTTTACAGGAAGCTGACATTATTTTAACCACTCCTGAGAAATTTGATGCTGTAACACGGTATCGCGTAAAGGATGGTGGATTGGGCTTTTTCAGCGATATAGCACTTCAACTTATTGATGAAGTTCATCTGTTGAATGATCCTCGTGGAGCAGTTTTGGAAGCAATAGTTAGCAGAATAAAAATGCTTGCTTACAACCCTGAAATGAAGTCAAGCGCTCTGGCTCATGTTCGTTTTCTAGCTGTGTCTGCCACCATTCCAAATATTGAGGACCTTGCGGAGTGGCTCAAGGTGCCTGTCCAAGGGATTAAAAGGTATGGAGAAGAAATGAGGCCTGTAAAGCTGACTACAAAAGCTACGCCCCAGCAAAAAAATAATTTTCTATTTGAAAAGCGCCTTCAAAACTATATTTTCGATATCCTTATGCAATATTCAAGAGGGAAGTCCACTGTAATTTTTTGCTTAACTAGAAAAGGTGCACAAGAAGCAGCACAGATACTTTCTCAGACAGTCATGGCCTTTGGTCATTCAAATCCATTCATTAAAAGCAGAGAACAGCAAGAGAGGCTAAGGGAAGCTTCATTGTCATGCAGTGACAAACAAATGCAATCTTATATGATTTATGGTGTTGGTTATCACAACGGTGGCCTTTGCCTCAAGGATCGTAATCTCATTGAAAGTCTTTTCCTTAAGGGTGATGTTCAAATTCTGTGCACAACAAATACTCTTGCTCATGGAATCAACCTACCAGCACATACAGTAGTCATAAAATCAACACAGCACTTCAACAAAGAAAAAGGAGTCTACATGGAAATTGACCGATCTACACTGCTACAGATGTGTGGGAGGGCAGGTCGCCCACCTTTTGATGATACAGGAATGGTTATAATCATGACAAGAAGAGATACGGTTCACTTGTATGAGAATCTCTTGAATGGATGTGAGATGGTGGAATCACAATTGCTTTCATGTTTGACGGAGCATTTAACTGCAGAGATAGTTCAACTGACTGTCTCTGACATTACAAGGGCAATCGAATGGATGAAGTGCTCTTTCTTGTATGTGAGAATGAAGAAGAATCCCGGACACTATGCAGTTAGAAAAGTGATTTCCAATGATCTTATAGAAAAGCATATGCTAGAGGTCTGTGTACAGAAAGTTAATGAGTTGTCGCAGCATCAGATGATATGGACTGATGAAGATGGTTTTCTGTTGAAACCACTAGAACCTGGGAGGCTGATGACGAATTACTACTTGAAATTTGACACAATGAAATACATTTTTCAGACACCTGCAAACTGCAGCTTGGAAGATGCTCTTCATGTGATATGCCGTGCTGAGGAAATTTTATGGATACAGCTCAGGCGCAATGAGAAAAAGCTTCTAAATGACATAAACACTGGTAAAGATGGTCGACTTCGCTTTCACATTCTTGGTGACAAAGGGAAGCGAAAAAAGCGTATTCAAACCAGAGAAGAAAAGATATTTCTTTTGGCTAATGACTGCTTGACTGGTGATCCATCGGTTCATGATTTATCCCTTACCCAGAATTGCCAGATGCATGAAAGAATTTTTTATTTACCAAAAGAATTACAAGGAGCCTTGAACTTGATGCTTTTGGCAAAGTCTATGTATCAGAAACTCTGGGATGACAGTCCATACTTGCTGAAACAATTACCAGGGATTGTAATGGTGACTGCAAAGGCACTACATTTGATGGGAGTTAAATCATTTGAGACACTAGCTGAGGCTGATCCTAGGAGAATCGAGATAGTCACTGGTCGAAAATACCCATATGGGAACCACATAAAAGAGTCTCTACGGTCTCTACCTCCAAAGATTGAGTTGAAAGTTGAGGAAACTGAATGCCGGGGGCAAGGGAAACTGAAGCTAGCGGTGACACTGACTAGGCTATCACAGACACTCCAATCAACAAAACGACATTATGCTGATATGATTGTAGGTTCAGAAGAAGATAACCTGGTTCTCTTTCATGAGAAAATAAGAGTTGATGAATTCTCGAGCCCCTATAGCGCAACAGTTCTCCTGTCAAATCCCCAACAAGGAAAGCTGACTGCTAAGGCTGATCTTTTATTTGAAGAATACATTGGTATTGATCTAAACCAGGAGCTTATCTTAATGAAGAAAAGCAATTTAAGTGCAAATAACAAACGAGGAAAAAAAGCACCCTCTCTTCCTCTACCTGAGGAAGTATGTATCATAGAAGATGATATTTATGAAGGCCCAAGTGAAGTGCTTCCAAACTCGACGAGATCCAAAAGGGTAGATGGTCCCATGCCCAGTTTCAAGCTTCTAGATGAAGACTCTGAAGAAGATGAGGTTGCCGACAAAGTTCATGAAGATGAGATTGCTGCCAAATTTCAAACTGATGACAAAGTTGTGACACAACAAACAGTATTTGACCACATACGTGAAAAGGCAAAGAACTTCCCTGTCTTGGTGTTATCAAATAGTGTGCTTCCTCCATCATCAGAGCCCTTGGAATTAGCAAGGAAACGTGCTCGTGAGAAGAAGCTTGGACCGGAAAGTGGAGTAGAGGTTCTGGAAGAATTAGAATGGAATAAAGTTCAAAGACGAACTGTGGTCAATCCATCTTCAGAACTTAAAGAAGCAGAACAACATCTTTCTAGTATCAAAAATCATCCAAATCCCAGAAGCTCAGCTGCCCTGAACGTCATGGATGGCAGAGGAGAAACAATATTCGAGCACATACGCACTAAAGCTAAGAATTTTCCGGTGATCAATAAACCAAATGTGGAGGAGGCTGAGTCTTTAATCCAGACAAAACAACGCCTATATAAGACTGAACCAGAGTTTAGCATGGATATTACTGCCGTGCTGAAAGGAAGAAAACCAAATGAGGTTATCAGAGATACTGAATGTGATAGGAGGTTGCTCTCCGGAAGCTCCTGCAACCCTCATGGAATATTGAATGCTTCTCCCGGAGGTTTAAGCAATGGAGCCAATCTCTCATCAAGTAATATGCTATCTTTCGATATTTCCATGTTCAAGAATCCGAAGCGACCTGCAGATCCAGCTAGCACTGTGGAGAATACTAGGAAGAAACATCAACACTCCAATTGTACCACCAAGGCACTGCTCGTTGACAGAATAATGGGTGCCCCTAAGATAGGACATGCTGCATATAAAGCTCCAAGTATACTCAAGGAGATCTTCTATGGGATAACTATTGGGCTCGCGGCCGGTTACCTGTGGAAAATGCATCACTGGAACGAGCAAAGGAGGAGAAGGGAGTTTTATGATCTGCTTGATGCAGGTAAAATTACTACCGTCGTCGACGAAGACGAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000280 GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005730 GO:0005737 GO:0006139 GO:0006259 GO:0006310 GO:0006725 GO:0006807 GO:0006996 GO:0007049 GO:0007127 GO:0007131 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010501 GO:0016043 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022402 GO:0022414 GO:0031974 GO:0031981 GO:0034641 GO:0035825 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0048285 GO:0051321 GO:0061982 GO:0070013 GO:0070035 GO:0071704 GO:0071840 GO:0090304 GO:0140013 GO:0140098 GO:1901360 GO:1903046

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD HHH_5 Helicase_C PPR Sec63

Protein Analysis

1399

Amino Acids

158.64

Weight (kDa)

8.65

Isoelectric Point (pI)

49.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF247	PF03140	60 - 99	8.6e-06	Plant protein of unknown function
DEAD	PF00270	190 - 379	6.4e-28	DEAD/DEAH box helicase
ResIII	PF04851	201 - 336	3e-07	Type III restriction enzyme, res subunit
Helicase_C	PF00271	495 - 574	3.9e-08	Helicase conserved C-terminal domain
WHD_SNRNP200	PF23445	606 - 685	1.5e-12	Winged Helix-turn-helix domain
Sec63	PF02889	695 - 998	3.4e-29	Sec63 Brl domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0002006)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G27730 AT3G27730 AT3G27730 AT3G27730 AT3G27730 AT3G27730 AT3G27730
fragaria_vesca	FvH4_6g51100
malus_domestica	MD09G1029000.v1.1 MD09G1029100.v1.1
prunus_persica	Prupe.3G288900_v2.0.a1
pyrus_communis	pycom111g02300 pycom111g02310
rosa_chinensis	RchiOBHm_Chr2g0089321 RchiOBHm_Chr2g0172661 RchiOBHm_Chr2g0172671
rosa_laevigata	RLG00000022075 RLG00000022122 RLG00000022126
rosa_multiflora	Rmu_sc0002053.1_g000008
rosa_roxburghii	Rroxscaffold_2G00079680 Rroxscaffold_2G00080200 Rroxscaffold_2G00080420
rosa_rugosa	Rorug01G0489000 Rorug01G0489100 Rorug02G0246000 Rorug02G0560500.1
rosa_samantha	Rh2AG637300 Rh2AG637400 Rh2AG637500 Rh2AG637900 Rh2BG650700 Rh2BG650800 Rh2BG650900 Rh2BG651500 Rh2CG045200 Rh2CG045300 Rh2CG610500 Rh2CG617200 Rh2CG617600 Rh2DG658400 Rh2DG664700 Rh2DG665400
rosa_wichuraiana	Rw2G052240 Rw2G052720

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1751
AarI	CACCTGC	1 cut(s)	2164
Acc36I	ACCTGC	4 cut(s)	1706, 2164, 3919, 4151
Acc65I	GGTACC	1 cut(s)	449
AccB1I	GGYRCC	3 cut(s)	449, 1156, 4004
AccI	GTMKAC	5 cut(s)	500, 1668, 2278, 4045, 4182
AccII	CGCG	4 cut(s)	404, 410, 930, 4085
AccIII	TCCGGA	1 cut(s)	3785
AciI	CCGC	4 cut(s)	349, 1142, 2759, 4085
AclWI	GGATC	7 cut(s)	814, 998, 1533, 2375, 2612, 3132, 3911
AcoI	YGGCCR	2 cut(s)	444, 4086
AcuI	CTGAAG	3 cut(s)	2771, 3213, 3465
AfaI	GTAC	3 cut(s)	451, 2016, 3970
AfeI	AGCGCT	1 cut(s)	1081
AfiI	CCNNNNNNNGG	8 cut(s)	478, 760, 1166, 2203, 2927, 3322, 3413, 4016
AflII	CTTAAG	2 cut(s)	506, 1553
AjnI	CCWGG	6 cut(s)	471, 860, 2092, 2542, 2844, 2988
AjuI	GAANNNNNNNTTGG	2 cut(s)	2955, 2987
AleI	CACNNNNGTG	2 cut(s)	1506, 3294
AloI	GAACNNNNNNTCC	2 cut(s)	3408, 3440
Alw21I	GWGCWC	7 cut(s)	123, 834, 1345, 1580, 1916, 3388, 3594
Alw26I	GTCTC	5 cut(s)	834, 1882, 2594, 2685, 2694
Alw44I	GTGCAC	2 cut(s)	1341, 1576
AlwI	GGATC	7 cut(s)	814, 998, 1533, 2375, 2612, 3132, 3911
Ama87I	CYCGRG	2 cut(s)	810, 2884
Aor13HI	TCCGGA	1 cut(s)	3785
Aor51HI	AGCGCT	1 cut(s)	1081
AoxI	GGCC	7 cut(s)	350, 444, 1196, 1380, 1510, 3106, 4086
ApaLI	GTGCAC	2 cut(s)	1341, 1576
ApeKI	GCWGC	7 cut(s)	68, 1352, 2038, 2166, 3236, 3548, 4025
Asp700I	GAANNNNTTC	2 cut(s)	585, 3120
Asp718I	GGTACC	1 cut(s)	449
AspA2I	CCTAGG	1 cut(s)	2621
AspLEI	GCGC	7 cut(s)	406, 412, 631, 1082, 1260, 2232, 2900
AspS9I	GGNCC	6 cut(s)	351, 792, 1135, 3107, 3155, 3404
AsuC2I	CCSGG	3 cut(s)	1947, 2735, 3828
AsuHPI	GGTGA	8 cut(s)	356, 826, 1571, 2312, 2390, 2569, 2773, 3634
AsuNHI	GCTAGC	2 cut(s)	2755, 3923
AvaI	CYCGRG	2 cut(s)	810, 2884
AvaII	GGWCC	4 cut(s)	792, 1135, 3155, 3404
AvrII	CCTAGG	1 cut(s)	2621
AxyI	CCTNAGG	1 cut(s)	3069
BaeGI	GKGCMC	3 cut(s)	1345, 1580, 4009
BaeI	ACNNNNGTAYC	2 cut(s)	365, 398
BanI	GGYRCC	3 cut(s)	449, 1156, 4004
BanII	GRGCYC	4 cut(s)	834, 2891, 3363, 4083
BauI	CACGAG	2 cut(s)	1008, 3387
BbsI	GAAGAC	3 cut(s)	108, 305, 3192
Bbv12I	GWGCWC	7 cut(s)	123, 834, 1345, 1580, 1916, 3388, 3594
BbvCI	CCTCAGC	2 cut(s)	2202, 2610
BbvI	GCAGC	7 cut(s)	80, 1364, 2050, 2178, 3223, 3535, 4012
BceAI	ACGGC	3 cut(s)	459, 2181, 3704
BciT130I	CCWGG	6 cut(s)	473, 862, 2094, 2544, 2846, 2990
BciVI	GTATCC	1 cut(s)	2211
BclI	TGATCA	1 cut(s)	3624
BcnI	CCSGG	3 cut(s)	1947, 2735, 3828
BcoDI	GTCTC	5 cut(s)	834, 1882, 2594, 2685, 2694
BfmI	CTRYAG	5 cut(s)	1697, 1854, 2164, 2893, 3912
BfoI	RGCGCY	3 cut(s)	632, 1083, 1261
BfrI	CTTAAG	2 cut(s)	506, 1553
BfuAI	ACCTGC	4 cut(s)	1706, 2164, 3919, 4151
BfuI	GTATCC	1 cut(s)	2211
BglII	AGATCT	1 cut(s)	4056
BisI	GCNGC	9 cut(s)	69, 350, 1353, 2039, 2167, 3237, 3549, 4026, 4086
BlnI	CCTAGG	1 cut(s)	2621
BlpI	GCTNAGC	1 cut(s)	3544
BlsI	GCNGC	9 cut(s)	70, 351, 1354, 2040, 2168, 3238, 3550, 4027, 4087
Bme1390I	CCNGG	9 cut(s)	473, 862, 1947, 2094, 2544, 2735, 2846, 2990, 3828
Bme18I	GGWCC	4 cut(s)	792, 1135, 3155, 3404
BmeT110I	CYCGRG	2 cut(s)	810, 2884
BmgT120I	GGNCC	6 cut(s)	351, 792, 1135, 3107, 3155, 3404
BmrFI	CCNGG	9 cut(s)	473, 862, 1947, 2094, 2544, 2735, 2846, 2990, 3828
BmrI	ACTGGG	1 cut(s)	3159
BmsI	GCATC	7 cut(s)	901, 2050, 2167, 2412, 2466, 4117, 4147
BmtI	GCTAGC	2 cut(s)	2759, 3927
BmuI	ACTGGG	1 cut(s)	3159
BpiI	GAAGAC	3 cut(s)	108, 305, 3192
BplI	GAGNNNNNCTC	2 cut(s)	3837, 3869
Bpu10I	CCTNAGC	5 cut(s)	1433, 2202, 2225, 2610, 2943
Bpu1102I	GCTNAGC	1 cut(s)	3544
BpuEI	CTTGAG	3 cut(s)	1136, 1505, 4034
BpuMI	CCSGG	3 cut(s)	1947, 2735, 3828
BsaAI	YACGTR	1 cut(s)	3296
BsaBI	GATNNNNATC	1 cut(s)	1530
BsaI	GGTCTC	1 cut(s)	2694
BsaJI	CCNNGG	8 cut(s)	513, 1165, 2093, 2543, 2621, 2734, 3363, 3975
BsaWI	WCCGGW	5 cut(s)	452, 836, 3406, 3619, 3785
BsaXI	ACNNNNNCTCC	8 cut(s)	1702, 1732, 2772, 2802, 3408, 3438, 3638, 3668
Bsc4I	CCNNNNNNNGG	8 cut(s)	478, 760, 1166, 2203, 2927, 3322, 3413, 4016
Bse118I	RCCGGY	3 cut(s)	446, 526, 4088
Bse1I	ACTGG	6 cut(s)	640, 2269, 2380, 2647, 3165, 4118
Bse21I	CCTNAGG	1 cut(s)	3069
Bse3DI	GCAATG	2 cut(s)	2239, 3847
Bse8I	GATNNNNATC	1 cut(s)	1530
BseAI	TCCGGA	1 cut(s)	3785
BseBI	CCWGG	6 cut(s)	473, 862, 2094, 2544, 2846, 2990
BseDI	CCNNGG	8 cut(s)	513, 1165, 2093, 2543, 2621, 2734, 3363, 3975
BseGI	GGATG	7 cut(s)	709, 943, 1808, 1911, 2518, 3526, 3571
BseJI	GATNNNNATC	1 cut(s)	1530
BseLI	CCNNNNNNNGG	8 cut(s)	478, 760, 1166, 2203, 2927, 3322, 3413, 4016
BseMI	GCAATG	2 cut(s)	2239, 3847
BseNI	ACTGG	6 cut(s)	640, 2269, 2380, 2647, 3165, 4118
BseRI	GAGGAG	3 cut(s)	3588, 3659, 4144
BseSI	GKGCMC	3 cut(s)	1345, 1580, 4009
BseX3I	CGGCCG	2 cut(s)	444, 4086
BseXI	GCAGC	7 cut(s)	80, 1364, 2050, 2178, 3223, 3535, 4012
BseYI	CCCAGC	1 cut(s)	1227
Bsh1236I	CGCG	4 cut(s)	404, 410, 930, 4085
Bsh1285I	CGRYCG	3 cut(s)	428, 447, 4089
BshFI	GGCC	7 cut(s)	352, 446, 1198, 1382, 1512, 3108, 4088
BshNI	GGYRCC	3 cut(s)	449, 1156, 4004
BsiEI	CGRYCG	3 cut(s)	428, 447, 4089
BsiHKAI	GWGCWC	7 cut(s)	123, 834, 1345, 1580, 1916, 3388, 3594
BsiHKCI	CYCGRG	2 cut(s)	810, 2884
BslFI	GGGAC	2 cut(s)	212, 3141
BslI	CCNNNNNNNGG	8 cut(s)	478, 760, 1166, 2203, 2927, 3322, 3413, 4016
BsmAI	GTCTC	5 cut(s)	834, 1882, 2594, 2685, 2694
BsmFI	GGGAC	2 cut(s)	212, 3141
BsmI	GAATGC	4 cut(s)	32, 587, 2735, 3823
BsnI	GGCC	7 cut(s)	352, 446, 1198, 1382, 1512, 3108, 4088
Bso31I	GGTCTC	1 cut(s)	2694
BsoBI	CYCGRG	2 cut(s)	810, 2884
Bsp13I	TCCGGA	1 cut(s)	3785
Bsp1407I	TGTACA	1 cut(s)	2014
Bsp1720I	GCTNAGC	1 cut(s)	3544
Bsp19I	CCATGG	1 cut(s)	513
BspACI	CCGC	4 cut(s)	349, 1142, 2759, 4085
BspANI	GGCC	7 cut(s)	352, 446, 1198, 1382, 1512, 3108, 4088
BspEI	TCCGGA	1 cut(s)	3785
BspFNI	CGCG	4 cut(s)	404, 410, 930, 4085
BspHI	TCATGA	5 cut(s)	211, 1755, 2392, 2857, 3220
BspMAI	CTGCAG	3 cut(s)	1858, 2168, 3916
BspMI	ACCTGC	4 cut(s)	1706, 2164, 3919, 4151
BspOI	GCTAGC	2 cut(s)	2759, 3927
BspPI	GGATC	7 cut(s)	814, 998, 1533, 2375, 2612, 3132, 3911
BspQI	GCTCTTC	1 cut(s)	2187
BspT107I	GGYRCC	3 cut(s)	449, 1156, 4004
BspTI	CTTAAG	2 cut(s)	506, 1553
BspTNI	GGTCTC	1 cut(s)	2694
BsrDI	GCAATG	2 cut(s)	2239, 3847
BsrFI	RCCGGY	3 cut(s)	446, 526, 4088
BsrGI	TGTACA	1 cut(s)	2014
BsrI	ACTGG	6 cut(s)	640, 2269, 2380, 2647, 3165, 4118
BssAI	RCCGGY	3 cut(s)	446, 526, 4088
BssECI	CCNNGG	8 cut(s)	513, 1165, 2093, 2543, 2621, 2734, 3363, 3975
BssNAI	GTATAC	2 cut(s)	501, 4046
BssSI	CACGAG	2 cut(s)	1008, 3387
BssT1I	CCWWGG	5 cut(s)	513, 1165, 2621, 3363, 3975
Bst1107I	GTATAC	2 cut(s)	501, 4046
Bst2BI	CACGAG	2 cut(s)	1008, 3387
Bst2UI	CCWGG	6 cut(s)	473, 862, 2094, 2544, 2846, 2990
Bst6I	CTCTTC	7 cut(s)	42, 48, 366, 402, 1759, 2187, 3063
BstAFI	CTTAAG	2 cut(s)	506, 1553
BstAPI	GCANNNNNTGC	1 cut(s)	822
BstAUI	TGTACA	1 cut(s)	2014
BstBAI	YACGTR	1 cut(s)	3296
BstC8I	GCNNGC	6 cut(s)	408, 528, 1053, 2757, 3925, 4083
BstDSI	CCRYGG	1 cut(s)	513
BstEII	GGTNACC	1 cut(s)	4091
BstENI	CCTNNNNNAGG	2 cut(s)	1164, 4014
BstF5I	GGATG	7 cut(s)	709, 943, 1808, 1911, 2518, 3526, 3571
BstFNI	CGCG	4 cut(s)	404, 410, 930, 4085
BstH2I	RGCGCY	3 cut(s)	632, 1083, 1261
BstHHI	GCGC	7 cut(s)	406, 412, 631, 1082, 1260, 2232, 2900
BstMAI	GTCTC	5 cut(s)	834, 1882, 2594, 2685, 2694
BstMCI	CGRYCG	3 cut(s)	428, 447, 4089
BstNI	CCWGG	6 cut(s)	473, 862, 2094, 2544, 2846, 2990
BstNSI	RCATGY	2 cut(s)	546, 4025
BstPI	GGTNACC	1 cut(s)	4091
BstSCI	CCNGG	9 cut(s)	471, 860, 1945, 2092, 2542, 2733, 2844, 2988, 3826
BstSFI	CTRYAG	5 cut(s)	1697, 1854, 2164, 2893, 3912
BstSLI	GKGCMC	3 cut(s)	1345, 1580, 4009
BstUI	CGCG	4 cut(s)	404, 410, 930, 4085
BstV1I	GCAGC	7 cut(s)	80, 1364, 2050, 2178, 3223, 3535, 4012
BstV2I	GAAGAC	3 cut(s)	108, 305, 3192
BstX2I	RGATCY	4 cut(s)	806, 3137, 3916, 4056
BstXI	CCANNNNNNTGG	1 cut(s)	803
BstYI	RGATCY	4 cut(s)	806, 3137, 3916, 4056
BstZ17I	GTATAC	2 cut(s)	501, 4046
BstZI	CGGCCG	2 cut(s)	444, 4086
Bsu36I	CCTNAGG	1 cut(s)	3069
BsuI	GTATCC	1 cut(s)	2211
BsuRI	GGCC	7 cut(s)	352, 446, 1198, 1382, 1512, 3108, 4088
BtgI	CCRYGG	1 cut(s)	513
BtsCI	GGATG	7 cut(s)	709, 943, 1808, 1911, 2518, 3526, 3571
BtsI	GCAGTG	3 cut(s)	1462, 1691, 3980
BtsIMutI	CAGTG	9 cut(s)	1311, 1462, 1691, 2262, 2640, 2765, 3927, 3980, 4111
BveI	ACCTGC	4 cut(s)	1706, 2164, 3919, 4151
Cac8I	GCNNGC	6 cut(s)	408, 528, 1053, 2757, 3925, 4083
CciI	TCATGA	5 cut(s)	211, 1755, 2392, 2857, 3220
CfoI	GCGC	7 cut(s)	406, 412, 631, 1082, 1260, 2232, 2900
Cfr10I	RCCGGY	3 cut(s)	446, 526, 4088
Cfr13I	GGNCC	6 cut(s)	351, 792, 1135, 3107, 3155, 3404
CsiI	ACCWGGT	1 cut(s)	2844
Csp6I	GTAC	3 cut(s)	450, 2015, 3969
CviQI	GTAC	3 cut(s)	450, 2015, 3969
EaeI	YGGCCR	2 cut(s)	444, 4086
EagI	CGGCCG	2 cut(s)	444, 4086
Eam1104I	CTCTTC	7 cut(s)	42, 48, 366, 402, 1759, 2187, 3063
EarI	CTCTTC	7 cut(s)	42, 48, 366, 402, 1759, 2187, 3063
Ecl136II	GAGCTC	1 cut(s)	832
EclXI	CGGCCG	2 cut(s)	444, 4086
Eco130I	CCWWGG	5 cut(s)	513, 1165, 2621, 3363, 3975
Eco147I	AGGCCT	1 cut(s)	1198
Eco24I	GRGCYC	4 cut(s)	834, 2891, 3363, 4083
Eco31I	GGTCTC	1 cut(s)	2694
Eco32I	GATATC	1 cut(s)	1282
Eco47I	GGWCC	4 cut(s)	792, 1135, 3155, 3404
Eco47III	AGCGCT	1 cut(s)	1081
Eco52I	CGGCCG	2 cut(s)	444, 4086
Eco53kI	GAGCTC	1 cut(s)	832
Eco57I	CTGAAG	3 cut(s)	2771, 3213, 3465
Eco81I	CCTNAGG	1 cut(s)	3069
Eco88I	CYCGRG	2 cut(s)	810, 2884
Eco91I	GGTNACC	1 cut(s)	4091
EcoICRI	GAGCTC	1 cut(s)	832
EcoNI	CCTNNNNNAGG	2 cut(s)	1164, 4014
EcoO109I	RGGNCCY	1 cut(s)	1135
EcoO65I	GGTNACC	1 cut(s)	4091
EcoRI	GAATTC	1 cut(s)	2879
EcoRII	CCWGG	6 cut(s)	471, 860, 2092, 2542, 2844, 2988
EcoRV	GATATC	1 cut(s)	1282
EcoT14I	CCWWGG	5 cut(s)	513, 1165, 2621, 3363, 3975
EcoT22I	ATGCAT	2 cut(s)	2427, 4110
EcoT38I	GRGCYC	4 cut(s)	834, 2891, 3363, 4083
ErhI	CCWWGG	5 cut(s)	513, 1165, 2621, 3363, 3975
FalI	AAGNNNNNCTT	6 cut(s)	1426, 1458, 2166, 2198, 4043, 4075
FaqI	GGGAC	2 cut(s)	212, 3141
FauNDI	CATATG	2 cut(s)	1998, 2659
FbaI	TGATCA	1 cut(s)	3624
FblI	GTMKAC	5 cut(s)	500, 1668, 2278, 4045, 4182
Fnu4HI	GCNGC	9 cut(s)	69, 350, 1353, 2039, 2167, 3237, 3549, 4026, 4086
FokI	GGATG	7 cut(s)	716, 950, 1815, 1918, 2525, 3513, 3578
FriOI	GRGCYC	4 cut(s)	834, 2891, 3363, 4083
Fsp4HI	GCNGC	9 cut(s)	69, 350, 1353, 2039, 2167, 3237, 3549, 4026, 4086
GlaI	GCGC	7 cut(s)	405, 411, 630, 1081, 1259, 2231, 2899
GluI	GCNGC	9 cut(s)	69, 350, 1353, 2039, 2167, 3237, 3549, 4026, 4086
GsaI	CCCAGC	1 cut(s)	1231
HaeII	RGCGCY	3 cut(s)	632, 1083, 1261
HaeIII	GGCC	7 cut(s)	352, 446, 1198, 1382, 1512, 3108, 4088
HhaI	GCGC	7 cut(s)	406, 412, 631, 1082, 1260, 2232, 2900
Hin6I	GCGC	7 cut(s)	404, 410, 629, 1080, 1258, 2230, 2898
HinP1I	GCGC	7 cut(s)	404, 410, 629, 1080, 1258, 2230, 2898
HincII	GTYRAC	4 cut(s)	112, 2279, 3991, 4183
HindII	GTYRAC	4 cut(s)	112, 2279, 3991, 4183
HindIII	AAGCTT	5 cut(s)	206, 1440, 2243, 3173, 3398
HphI	GGTGA	8 cut(s)	356, 826, 1571, 2312, 2390, 2569, 2773, 3634
Hpy99I	CGWCG	5 cut(s)	445, 3136, 4181, 4184, 4187
HpyAV	CCTTC	7 cut(s)	284, 327, 596, 772, 1271, 3098, 4128
HpyCH4IV	ACGT	3 cut(s)	3295, 3382, 3558
HpySE526I	ACGT	3 cut(s)	3295, 3382, 3558
HspAI	GCGC	7 cut(s)	404, 410, 629, 1080, 1258, 2230, 2898
Kpn2I	TCCGGA	1 cut(s)	3785
KpnI	GGTACC	1 cut(s)	453
KroI	GCCGGC	1 cut(s)	526
KroNI	GCCGGC	1 cut(s)	528
Ksp22I	TGATCA	1 cut(s)	3624
LguI	GCTCTTC	1 cut(s)	2187
Lsp1109I	GCAGC	7 cut(s)	80, 1364, 2050, 2178, 3223, 3535, 4012
LweI	GCATC	7 cut(s)	901, 2050, 2167, 2412, 2466, 4117, 4147
MabI	ACCWGGT	1 cut(s)	2844
MaeII	ACGT	3 cut(s)	3295, 3382, 3558
MaeIII	GTNAC	8 cut(s)	916, 1457, 2300, 2557, 2638, 2761, 3265, 4091
MfeI	CAATTG	2 cut(s)	1823, 3964
MflI	RGATCY	4 cut(s)	806, 3137, 3916, 4056
MlyI	GAGTC	5 cut(s)	160, 1674, 2687, 3182, 3662
Mph1103I	ATGCAT	2 cut(s)	2427, 4110
MroI	TCCGGA	1 cut(s)	3785
MroNI	GCCGGC	1 cut(s)	526
MroXI	GAANNNNTTC	2 cut(s)	585, 3120
MslI	CAYNNNNRTG	7 cut(s)	606, 620, 1450, 1506, 1782, 3225, 3294
MspA1I	CMGCKG	1 cut(s)	3548
MspCI	CTTAAG	2 cut(s)	506, 1553
MspR9I	CCNGG	9 cut(s)	473, 862, 1947, 2094, 2544, 2735, 2846, 2990, 3828
MunI	CAATTG	2 cut(s)	1823, 3964
Mva1269I	GAATGC	4 cut(s)	32, 587, 2735, 3823
MvaI	CCWGG	6 cut(s)	473, 862, 2094, 2544, 2846, 2990
MvnI	CGCG	4 cut(s)	404, 410, 930, 4085
NaeI	GCCGGC	1 cut(s)	528
NciI	CCSGG	3 cut(s)	1947, 2735, 3828
NcoI	CCATGG	1 cut(s)	513
NdeI	CATATG	2 cut(s)	1998, 2659
NgoMIV	GCCGGC	1 cut(s)	526
NheI	GCTAGC	2 cut(s)	2755, 3923
NmuCI	GTSAC	6 cut(s)	1457, 2300, 2557, 2638, 2761, 3265
NsiI	ATGCAT	2 cut(s)	2427, 4110
NspI	RCATGY	2 cut(s)	546, 4025
OliI	CACNNNNGTG	2 cut(s)	1506, 3294
PaeR7I	CTCGAG	1 cut(s)	2884
PagI	TCATGA	5 cut(s)	211, 1755, 2392, 2857, 3220
PaqCI	CACCTGC	1 cut(s)	2164
PceI	AGGCCT	1 cut(s)	1198
PciSI	GCTCTTC	1 cut(s)	2187
PcsI	WCGNNNNNNNCGW	1 cut(s)	4188
PctI	GAATGC	4 cut(s)	32, 587, 2735, 3823
PdiI	GCCGGC	1 cut(s)	528
PdmI	GAANNNNTTC	2 cut(s)	585, 3120
PflFI	GACNNNGTC	1 cut(s)	107
PfoI	TCCNGGA	3 cut(s)	471, 1945, 3826
PkrI	GCNGC	9 cut(s)	70, 351, 1354, 2040, 2168, 3238, 3550, 4027, 4087
Ple19I	CGATCG	1 cut(s)	428
PleI	GAGTC	5 cut(s)	160, 1673, 2686, 3182, 3661
PpsI	GAGTC	5 cut(s)	160, 1673, 2686, 3182, 3661
Ppu21I	YACGTR	1 cut(s)	3296
PpuMI	RGGWCCY	1 cut(s)	1135
PsiI	TTATAA	1 cut(s)	1751
Psp124BI	GAGCTC	1 cut(s)	834
Psp5II	RGGWCCY	1 cut(s)	1135
Psp6I	CCWGG	6 cut(s)	471, 860, 2092, 2542, 2844, 2988
PspEI	GGTNACC	1 cut(s)	4091
PspFI	CCCAGC	1 cut(s)	1227
PspGI	CCWGG	6 cut(s)	471, 860, 2092, 2542, 2844, 2988
PspPI	GGNCC	6 cut(s)	351, 792, 1135, 3107, 3155, 3404
PspPPI	RGGWCCY	1 cut(s)	1135
PstI	CTGCAG	3 cut(s)	1858, 2168, 3916
PsuI	RGATCY	4 cut(s)	806, 3137, 3916, 4056
PsyI	GACNNNGTC	1 cut(s)	107
PvuI	CGATCG	1 cut(s)	428
PvuII	CAGCTG	1 cut(s)	3548
RsaI	GTAC	3 cut(s)	451, 2016, 3970
RsaNI	GTAC	3 cut(s)	450, 2015, 3969
RseI	CAYNNNNRTG	7 cut(s)	606, 620, 1450, 1506, 1782, 3225, 3294
SacI	GAGCTC	1 cut(s)	834
SalI	GTCGAC	2 cut(s)	2277, 4181
SapI	GCTCTTC	1 cut(s)	2187
SatI	GCNGC	9 cut(s)	69, 350, 1353, 2039, 2167, 3237, 3549, 4026, 4086
Sau96I	GGNCC	6 cut(s)	351, 792, 1135, 3107, 3155, 3404
SchI	GAGTC	5 cut(s)	160, 1674, 2687, 3182, 3662
ScrFI	CCNGG	9 cut(s)	473, 862, 1947, 2094, 2544, 2735, 2846, 2990, 3828
SexAI	ACCWGGT	1 cut(s)	2844
SfaNI	GCATC	7 cut(s)	901, 2050, 2167, 2412, 2466, 4117, 4147
SfcI	CTRYAG	5 cut(s)	1697, 1854, 2164, 2893, 3912
Sfr274I	CTCGAG	1 cut(s)	2884
SgrDI	CGTCGACG	1 cut(s)	4181
SinI	GGWCC	4 cut(s)	792, 1135, 3155, 3404
SlaI	CTCGAG	1 cut(s)	2884
SmiMI	CAYNNNNRTG	7 cut(s)	606, 620, 1450, 1506, 1782, 3225, 3294
SmlI	CTYRAG	6 cut(s)	506, 1151, 1520, 1553, 2884, 4049
SmoI	CTYRAG	6 cut(s)	506, 1151, 1520, 1553, 2884, 4049
SseBI	AGGCCT	1 cut(s)	1198
SsiI	CCGC	4 cut(s)	349, 1142, 2759, 4085
SspI	AATATT	5 cut(s)	130, 1129, 1295, 3585, 3813
SstI	GAGCTC	1 cut(s)	834
StuI	AGGCCT	1 cut(s)	1198
StyD4I	CCNGG	9 cut(s)	471, 860, 1945, 2092, 2542, 2733, 2844, 2988, 3826
StyI	CCWWGG	5 cut(s)	513, 1165, 2621, 3363, 3975
TaiI	ACGT	3 cut(s)	3298, 3385, 3561
TaqII	GACCGA	1 cut(s)	1698
TatI	WGTACW	1 cut(s)	2014
TauI	GCSGC	2 cut(s)	352, 4088
TscAI	CASTG	9 cut(s)	1318, 1462, 1698, 2269, 2647, 2772, 3934, 3987, 4118
TseFI	GTSAC	6 cut(s)	1457, 2300, 2557, 2638, 2761, 3265
TseI	GCWGC	7 cut(s)	68, 1352, 2038, 2166, 3236, 3548, 4025
Tsp45I	GTSAC	6 cut(s)	1457, 2300, 2557, 2638, 2761, 3265
TspGWI	ACGGA	3 cut(s)	20, 233, 1856
TspRI	CASTG	9 cut(s)	1318, 1462, 1698, 2269, 2647, 2772, 3934, 3987, 4118
Tth111I	GACNNNGTC	1 cut(s)	107
Vha464I	CTTAAG	2 cut(s)	506, 1553
VneI	GTGCAC	2 cut(s)	1341, 1576
VpaK11BI	GGWCC	4 cut(s)	792, 1135, 3155, 3404
XagI	CCTNNNNNAGG	2 cut(s)	1164, 4014
XbaI	TCTAGA	1 cut(s)	3178
XceI	RCATGY	2 cut(s)	546, 4025
XhoI	CTCGAG	1 cut(s)	2884
XmaJI	CCTAGG	1 cut(s)	2621
XmiI	GTMKAC	5 cut(s)	500, 1668, 2278, 4045, 4182
XmnI	GAANNNNTTC	2 cut(s)	585, 3120
Zsp2I	ATGCAT	2 cut(s)	2427, 4110

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.