Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0002006

Physical Location & Seq

Genomic Coordinates Forward (+)

2650451 .. 2660411

Gene Structure 9961 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00079680.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3771 bp

ATGGAGAAATTCGAGGTTCAGGAAACGGGTTCAAGCATGGATTCGTATACACTTAAGTCCATGGCAGACTTGCCGGCTCCTTTTCGAGCATGTTTTAATTTTAGGTATTTCAACTCTTTGCAGAGTGAATGCTTTCCTGCTTGTTTCCACTCAGATGTGAACATGGTTGTGTCAGCGCCAACTGGAAGTGGTAAAACAGTCATTTTTGAGCTTTGCATTTTGAGGCTTCTTTCAAGATTCATCTCTGAGGATGGGAGGTTTACACCTACAAATGGAACTCTCAAAACAATCTATATTGCCCCATCAAAGGCTTTAGTTCAGGAGAAGGTTCGTGATTGGACCCAGAAATTTGGATCTCGGGGCATAGTTTGTTTGGAGCTCACTGGAGATAATGAATCTTACAACACCAGGAGTTTACAGGAAGCAGACATTATTTTAACCACTCCTGAGAAATTTGATGCTGTAACGCGGTATCGCGTAAAGGATGGTGGATTGGGCTTTTTCAGCGATATAGCACTTCTACTTATTGATGAAGTTCATCTGTTGAATGATCCTCGTGGAGCAGTTTTGGAAGCAATAGTTAGCAGAATAAAAATGCTTGCTTACAACCCTGAAATGAAGTCAAGCGCTCTGGCTCATGTTCGTTTTCTAGCTGTGTCTGCCACCATTCCAAATATTGAGGACCTTGCGGAGTGGCTCAAGGTGCCTGTCCAAGGGATTAAAAGGTTTGGAGAAGAAATGAGGCCTGTAAAGCTGACTACAAAAGTTTTTGGCTACGCCCCAGCAAAAAATGATTTTCTATTTGAAAAGCGCCTTCAAAACTACATTTTCGATATCCTTATGCAATATTCAAGAGGGAAGTCCGCTCTAATTTTTTGCTCAACTAGAAAAGGTGCACAAGAAGCAGCACAGATACTTTCTCAGACAGTCATGGCCTTTGGTCATTCAAATCCATTCATTAAAAGCAGAGAACAGCAAGAGAGGCTAAGGGAAGCTTCATTGTCATGCAGTGACAAACAAATGCAATCTTATATGATTTATGGTGTTGGTTATCACAATGGTGGCCTTTGCCTCAAGGATCGTAATCTCATTGAAAGTCTTTTCCTTAAGGGTGATGTTCAAATTCTGTGCACAACAAATACTCTTGCTCACGGAATCAACCTACCAGCACATACAGTAGTCATAAAATCAACACAGCACTTCAACAAAGAAAAAGGAGTCTACATGGAAATTGACCGATCTACACTGCTACAGTATATATCTTGTCTGTACATCAAGGCTCTATTTGAAATAAATGTGCCAGGTCAAACAAGAAAAATTCTTCTGGTGTCTACATGCATGTCAGCACTTTTAGAAACTTTTGTCTTCGAATTCGTTAATCGTGTTCTTATTGGATATCAGGTTCACTTGTATGAGAATCTCTTGAATGGATGTGAGATGGTGGAATCACAATTGCTTTCATGTTTGACGGAGCATTTAACTGCAGAGATAGTTCAACTGACTGTCTCTGACATTACAAGGGCGATCGAATGGATGAAGTGCTCTTTCTTGTATGTGAGAATGAAGAAGAATTCCGGACACTATGCAGTTAGAAAAGTGATTTCCAATGATCGTATAGAAAAGCATATGCTAGAGGTCTGTGTACAGAAAGTTAATGAGTTGTCGCAGCATCAGATGATATGGACTGATGAAGATGGTTTTCTGTTGAAACCACTAGAACCTGGGAGGCTGATGACGAAGTACTATTTGAAATTTGACACAATGAAATACATTATTCAGACACCTGCAAACTGCAGCTTGGAAGATGCTCTTCATGTGATATGCCGTGCTGAGGAAATTTCATGGATACAGCTCAGGCGCAATGAGAAAAAGCTTCTAAATGACATAAACACTGATAAAGATGGTCGACTTCGCTTTCACATTCTTGGTGACAAAGGGAAGCGAAAAAGCGATATGAACTCCATATGCTCAAATGGCTGTAGAATTGCCAGATGCATGAAAGAATATTTTATTTACCAAAAGAATTACAAAGGAGCCTTGAACTCGATGCTTTTGGCAAAGTCTATGTATCAGAAACTCTGGGATGACAGTCCATACTTGCTGAAACAATTACCAGGGATTGGAATGGTGACTGCAAAGGCACTACATTCGATGGGAGTTAAATCATTTGAGACACTAGCTGAGGCTGATCCTAGGAGAATTGAGATAGTCACCGGTCGAAAATACCCATATGGGAACCATATAAAAGAGTCTCTACTGTCTCTACCTCCAAAGGTTGAGTTGAAAGTTGAGGAAACTGAATGCCTGGGGCAAGGGAAACTGAAGCTAGCGGTGACACTGACTAGGCTATCACAGACACTCCAATCAACAAAACGACATTATGCTGATATGATTGTAGGTTCAGAAGAAGATAACCTGGTTCTCTTTCATGAGAAAATAAGAGTTGATGAATTCTCGAGCCCCTATAGCGCAACAGTTCTCCTGTCAAATCCCCAACAAAGAAAGCTGACTGTTAAGGCTGATCTTATATTTGAAGAATACAGTAAAGTCCCTGCCCTGATATTTCTAGTTTTCTTCACTTTACAAGTTACTATTGACTGGCCCAGTTTCAAGCTTCTAGATGAAGACTCTGAAGAAGATGAGGTTGCCGACAAAGTTCATGAAGATGAGATTGCTGCCAAAGTTCAAATTGATGACAAAGTTGTGACACAACAAACAGTATTTGACCACATACGTGAAAAGGCAAAGAACTTCCCTGTCTTTGTGTCATCAAATAGTGTGCTTCCTCCATCATCAGAGCCCTTGGAATTAGCAAGGAAACGTGCTCGTGAGAAGAAGCTTGGACCGGAAAGTGGAGTAGAGGTTCTGGAAGAATTAGAATGGAATAAAGTTCCAAGACGAACTGTGGTCAATCCATCTTCAGAACTTAAAGAAGCAGAACATCTTTCTAGTATCAAAAATCATCCAACTCCCAGAAGCTCAGCTGCCCTGAACGTCACGGATGGCAGAGACCCTGATGTCAAACCTCTAACAGGAGAAACAATATTCGAGCACATACGCACAAAAGCTAAGAATTTTCCGGTGATCAATAAACCAAATGTGGAGGAGGCTGAGTCTTTAATCCAGACAAAACAACGCCTATATAAGACTGAACCAGAGTTTAGCATGGATATTACTGCCGTGCTGAAAGGAAGAAAACCAAATGAGGTTATCAGAGATACTGAATGTGATAGGAGGTTGCTCTCCGGAAGCTCCTGCAACCCTTATGGAATATTGAATGCTTCTCCCGGAGGTTTAAGCAATGGAGCCAATCTCTCATCAAGTAACATGCCATCTTTCGATATCTCCACGTTCAAGAATCCAAAGCGACCTGTGGAGAATACTAGGAAGAAACATCAACACTCTCCAATTGTACCACCAAGGCACTGCTCGTTGACAGCAGTTGGAAAATCCAGAGAAGTAGAAACATTTCTTGGCCCACTGCTACTAAACACTTCCACACCAACTTTCAATAGTGGTATCAAAGCCACAAATTCCAACAATGATCTTGTTGTGGATTATTCTGCAGGAATAATGGGTGCCCCTAAGATAGGACATGCTGCATATAAAGGTCCAAGTATACTCAAGGAGATCTTCTATGGGATAACTATTGGGCTCGCGGCCGGTTACCTGTGGAAAATGCATCACTGGAACGAGCAGAGGAGGAGAAGGGAGTTTTATGATCTGCTTGATGCAGGTAAAATTACTACCGTCGTCGACGAAGACGAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000280 GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005730 GO:0005737 GO:0006139 GO:0006259 GO:0006310 GO:0006725 GO:0006807 GO:0006996 GO:0007049 GO:0007127 GO:0007131 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010501 GO:0016043 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022402 GO:0022414 GO:0031974 GO:0031981 GO:0034641 GO:0035825 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0048285 GO:0051321 GO:0061982 GO:0070013 GO:0070035 GO:0071704 GO:0071840 GO:0090304 GO:0140013 GO:0140098 GO:1901360 GO:1903046

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD HHH_5 Helicase_C PPR Sec63

Protein Analysis

1256

Amino Acids

142.21

Weight (kDa)

8.34

Isoelectric Point (pI)

44.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	39 - 228	1.5e-29	DEAD/DEAH box helicase
ResIII	PF04851	50 - 185	2.5e-08	Type III restriction enzyme, res subunit
WHD_SNRNP200	PF23445	482 - 561	1.6e-11	Winged Helix-turn-helix domain
Sec63	PF02889	571 - 633	2.5e-14	Sec63 Brl domain
Sec63	PF02889	649 - 849	2.1e-17	Sec63 Brl domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002006)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G27730 AT3G27730 AT3G27730 AT3G27730 AT3G27730 AT3G27730 AT3G27730
fragaria_vesca	FvH4_6g51100
malus_domestica	MD09G1029000.v1.1 MD09G1029100.v1.1
prunus_persica	Prupe.3G288900_v2.0.a1
pyrus_communis	pycom111g02300 pycom111g02310
rosa_chinensis	RchiOBHm_Chr2g0089321 RchiOBHm_Chr2g0172661 RchiOBHm_Chr2g0172671
rosa_laevigata	RLG00000022075 RLG00000022122 RLG00000022126
rosa_multiflora	Rmu_sc0002053.1_g000008
rosa_roxburghii	Rroxscaffold_2G00079680 Rroxscaffold_2G00080200 Rroxscaffold_2G00080420
rosa_rugosa	Rorug01G0489000 Rorug01G0489100 Rorug02G0246000 Rorug02G0560500.1
rosa_samantha	Rh2AG637300 Rh2AG637400 Rh2AG637500 Rh2AG637900 Rh2BG650700 Rh2BG650800 Rh2BG650900 Rh2BG651500 Rh2CG045200 Rh2CG045300 Rh2CG610500 Rh2CG617200 Rh2CG617600 Rh2DG658400 Rh2DG664700 Rh2DG665400
rosa_wichuraiana	Rw2G052240 Rw2G052720

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1792
AasI	GACNNNNNNGTC	1 cut(s)	3017
Acc36I	ACCTGC	2 cut(s)	1792, 3725
AccB1I	GGYRCC	2 cut(s)	703, 3578
AccB7I	CCANNNNNTGG	1 cut(s)	2120
AccBSI	CCGCTC	1 cut(s)	866
AccI	GTMKAC	6 cut(s)	47, 1221, 1331, 1906, 3619, 3756
AccII	CGCG	3 cut(s)	469, 477, 3659
AccIII	TCCGGA	2 cut(s)	1574, 3245
AciI	CCGC	5 cut(s)	469, 689, 864, 2330, 3659
AclWI	GGATC	4 cut(s)	361, 545, 1086, 2183
AcoI	YGGCCR	1 cut(s)	3660
AcuI	CTGAAG	3 cut(s)	2342, 2652, 2904
AfaI	GTAC	4 cut(s)	1271, 1644, 1742, 3414
AfeI	AGCGCT	1 cut(s)	628
AfiI	CCNNNNNNNGG	8 cut(s)	272, 307, 713, 1831, 2120, 2852, 3032, 3590
AflII	CTTAAG	2 cut(s)	53, 1106
AgeI	ACCGGT	1 cut(s)	2213
AjnI	CCWGG	6 cut(s)	407, 1300, 1720, 2113, 2304, 2415
AjuI	GAANNNNNNNTTGG	2 cut(s)	3456, 3488
AleI	CACNNNNGTG	2 cut(s)	1059, 2733
AloI	GAACNNNNNNTCC	4 cut(s)	1386, 1418, 2847, 2879
Alw21I	GWGCWC	6 cut(s)	381, 898, 1133, 1544, 2827, 3054
Alw26I	GTCTC	5 cut(s)	1510, 2165, 2256, 2265, 3003
Alw44I	GTGCAC	2 cut(s)	894, 1129
AlwI	GGATC	4 cut(s)	361, 545, 1086, 2183
Ama87I	CYCGRG	2 cut(s)	357, 2455
Aor13HI	TCCGGA	2 cut(s)	1574, 3245
Aor51HI	AGCGCT	1 cut(s)	628
AoxI	GGCC	6 cut(s)	743, 933, 1063, 2600, 3475, 3660
ApaLI	GTGCAC	2 cut(s)	894, 1129
ApeKI	GCWGC	6 cut(s)	905, 1666, 1794, 2675, 2984, 3599
AsiGI	ACCGGT	1 cut(s)	2213
Asp700I	GAANNNNTTC	2 cut(s)	132, 3468
AspA2I	CCTAGG	1 cut(s)	2192
AspLEI	GCGC	5 cut(s)	178, 629, 813, 1860, 2471
AspS9I	GGNCC	6 cut(s)	339, 682, 2601, 2843, 3476, 3611
AsuC2I	CCSGG	1 cut(s)	3288
AsuHPI	GGTGA	6 cut(s)	1124, 1940, 2140, 2203, 2344, 3094
AsuII	TTCGAA	1 cut(s)	1368
AsuNHI	GCTAGC	1 cut(s)	2326
AvaI	CYCGRG	2 cut(s)	357, 2455
AvaII	GGWCC	4 cut(s)	339, 682, 2843, 3611
AvrII	CCTAGG	1 cut(s)	2192
BaeGI	GKGCMC	3 cut(s)	898, 1133, 3583
BanI	GGYRCC	2 cut(s)	703, 3578
BanII	GRGCYC	4 cut(s)	381, 2462, 2802, 3657
BauI	CACGAG	2 cut(s)	555, 2826
BbsI	GAAGAC	2 cut(s)	1357, 2631
Bbv12I	GWGCWC	6 cut(s)	381, 898, 1133, 1544, 2827, 3054
BbvCI	CCTCAGC	2 cut(s)	1830, 2181
BbvI	GCAGC	6 cut(s)	917, 1678, 1806, 2662, 2971, 3586
BceAI	ACGGC	2 cut(s)	1809, 3164
BcgI	CGANNNNNNTGC	2 cut(s)	2130, 2164
BciT130I	CCWGG	6 cut(s)	409, 1302, 1722, 2115, 2306, 2417
BciVI	GTATCC	1 cut(s)	1839
BclI	TGATCA	1 cut(s)	3084
BcnI	CCSGG	1 cut(s)	3288
BcoDI	GTCTC	5 cut(s)	1510, 2165, 2256, 2265, 3003
BfmI	CTRYAG	6 cut(s)	1250, 1482, 1792, 1978, 2464, 3564
BfoI	RGCGCY	3 cut(s)	179, 630, 814
BfrI	CTTAAG	2 cut(s)	53, 1106
BfuAI	ACCTGC	2 cut(s)	1792, 3725
BfuI	GTATCC	1 cut(s)	1839
BglII	AGATCT	1 cut(s)	3630
BisI	GCNGC	7 cut(s)	906, 1667, 1795, 2676, 2985, 3600, 3660
BlnI	CCTAGG	1 cut(s)	2192
BlpI	GCTNAGC	1 cut(s)	2980
BlsI	GCNGC	7 cut(s)	907, 1668, 1796, 2677, 2986, 3601, 3661
BmcAI	AGTACT	1 cut(s)	1742
Bme1390I	CCNGG	7 cut(s)	409, 1302, 1722, 2115, 2306, 2417, 3288
Bme18I	GGWCC	4 cut(s)	339, 682, 2843, 3611
BmeT110I	CYCGRG	2 cut(s)	357, 2455
BmgT120I	GGNCC	6 cut(s)	339, 682, 2601, 2843, 3476, 3611
BmiI	GGNNCC	7 cut(s)	78, 341, 705, 2035, 2237, 3307, 3580
BmrFI	CCNGG	7 cut(s)	409, 1302, 1722, 2115, 2306, 2417, 3288
BmrI	ACTGGG	1 cut(s)	2598
BmsI	GCATC	7 cut(s)	448, 1678, 1795, 1982, 2037, 3691, 3721
BmtI	GCTAGC	1 cut(s)	2330
BmuI	ACTGGG	1 cut(s)	2598
BpiI	GAAGAC	2 cut(s)	1357, 2631
BplI	GAGNNNNNCTC	2 cut(s)	3297, 3329
BpmI	CTGGAG	1 cut(s)	405
Bpu10I	CCTNAGC	4 cut(s)	986, 1830, 1853, 2181
Bpu1102I	GCTNAGC	1 cut(s)	2980
Bpu14I	TTCGAA	1 cut(s)	1368
BpuEI	CTTGAG	3 cut(s)	683, 1058, 3608
BpuMI	CCSGG	1 cut(s)	3288
BsaAI	YACGTR	1 cut(s)	2735
BsaBI	GATNNNNATC	1 cut(s)	1083
BsaI	GGTCTC	1 cut(s)	3003
BsaJI	CCNNGG	8 cut(s)	60, 712, 1721, 2114, 2192, 2305, 2802, 3419
BsaWI	WCCGGW	5 cut(s)	1574, 2213, 2845, 3079, 3245
BsaXI	ACNNNNNCTCC	6 cut(s)	2343, 2373, 2847, 2877, 3098, 3128
Bsc4I	CCNNNNNNNGG	8 cut(s)	272, 307, 713, 1831, 2120, 2852, 3032, 3590
Bse118I	RCCGGY	3 cut(s)	73, 2213, 3662
Bse1I	ACTGG	5 cut(s)	187, 388, 2603, 2604, 3692
Bse3DI	GCAATG	2 cut(s)	1867, 3307
Bse8I	GATNNNNATC	1 cut(s)	1083
BseAI	TCCGGA	2 cut(s)	1574, 3245
BseBI	CCWGG	6 cut(s)	409, 1302, 1722, 2115, 2306, 2417
BseDI	CCNNGG	8 cut(s)	60, 712, 1721, 2114, 2192, 2305, 2802, 3419
BseGI	GGATG	7 cut(s)	256, 490, 1436, 1539, 2089, 2962, 3007
BseJI	GATNNNNATC	1 cut(s)	1083
BseLI	CCNNNNNNNGG	8 cut(s)	272, 307, 713, 1831, 2120, 2852, 3032, 3590
BseMI	GCAATG	2 cut(s)	1867, 3307
BseMII	CTCAG	9 cut(s)	165, 237, 438, 935, 1821, 1867, 2172, 2994, 3102
BseNI	ACTGG	5 cut(s)	187, 388, 2603, 2604, 3692
BseRI	GAGGAG	3 cut(s)	3119, 3715, 3718
BseSI	GKGCMC	3 cut(s)	898, 1133, 3583
BseX3I	CGGCCG	1 cut(s)	3660
BseXI	GCAGC	6 cut(s)	917, 1678, 1806, 2662, 2971, 3586
BseYI	CCCAGC	1 cut(s)	781
Bsh1236I	CGCG	3 cut(s)	469, 477, 3659
Bsh1285I	CGRYCG	3 cut(s)	1527, 2218, 3663
BshFI	GGCC	6 cut(s)	745, 935, 1065, 2602, 3477, 3662
BshNI	GGYRCC	2 cut(s)	703, 3578
BshTI	ACCGGT	1 cut(s)	2213
BsiEI	CGRYCG	3 cut(s)	1527, 2218, 3663
BsiHKAI	GWGCWC	6 cut(s)	381, 898, 1133, 1544, 2827, 3054
BsiHKCI	CYCGRG	2 cut(s)	357, 2455
BsiSI	CCGG	8 cut(s)	74, 1575, 2214, 2846, 3080, 3246, 3288, 3663
BslFI	GGGAC	1 cut(s)	2534
BslI	CCNNNNNNNGG	8 cut(s)	272, 307, 713, 1831, 2120, 2852, 3032, 3590
BsmAI	GTCTC	5 cut(s)	1510, 2165, 2256, 2265, 3003
BsmFI	GGGAC	1 cut(s)	2534
BsmI	GAATGC	3 cut(s)	134, 2306, 3283
BsnI	GGCC	6 cut(s)	745, 935, 1065, 2602, 3477, 3662
Bso31I	GGTCTC	1 cut(s)	3003
BsoBI	CYCGRG	2 cut(s)	357, 2455
Bsp119I	TTCGAA	1 cut(s)	1368
Bsp13I	TCCGGA	2 cut(s)	1574, 3245
Bsp1407I	TGTACA	2 cut(s)	1269, 1642
Bsp1720I	GCTNAGC	1 cut(s)	2980
Bsp19I	CCATGG	1 cut(s)	60
BspACI	CCGC	5 cut(s)	469, 689, 864, 2330, 3659
BspANI	GGCC	6 cut(s)	745, 935, 1065, 2602, 3477, 3662
BspCNI	CTCAG	9 cut(s)	164, 238, 439, 934, 1822, 1866, 2173, 2993, 3103
BspEI	TCCGGA	2 cut(s)	1574, 3245
BspFNI	CGCG	3 cut(s)	469, 477, 3659
BspHI	TCATGA	2 cut(s)	2428, 2659
BspLI	GGNNCC	7 cut(s)	78, 341, 705, 2035, 2237, 3307, 3580
BspMAI	CTGCAG	3 cut(s)	1486, 1796, 3568
BspMI	ACCTGC	2 cut(s)	1792, 3725
BspOI	GCTAGC	1 cut(s)	2330
BspPI	GGATC	4 cut(s)	361, 545, 1086, 2183
BspQI	GCTCTTC	1 cut(s)	1815
BspT104I	TTCGAA	1 cut(s)	1368
BspT107I	GGYRCC	2 cut(s)	703, 3578
BspTI	CTTAAG	2 cut(s)	53, 1106
BspTNI	GGTCTC	1 cut(s)	3003
BsrBI	CCGCTC	1 cut(s)	866
BsrDI	GCAATG	2 cut(s)	1867, 3307
BsrFI	RCCGGY	3 cut(s)	73, 2213, 3662
BsrGI	TGTACA	2 cut(s)	1269, 1642
BsrI	ACTGG	5 cut(s)	187, 388, 2603, 2604, 3692
BssAI	RCCGGY	3 cut(s)	73, 2213, 3662
BssECI	CCNNGG	8 cut(s)	60, 712, 1721, 2114, 2192, 2305, 2802, 3419
BssNAI	GTATAC	2 cut(s)	48, 3620
BssSI	CACGAG	2 cut(s)	555, 2826
BssT1I	CCWWGG	5 cut(s)	60, 712, 2192, 2802, 3419
Bst1107I	GTATAC	2 cut(s)	48, 3620
Bst2BI	CACGAG	2 cut(s)	555, 2826
Bst2UI	CCWGG	6 cut(s)	409, 1302, 1722, 2115, 2306, 2417
Bst6I	CTCTTC	1 cut(s)	1815
BstAFI	CTTAAG	2 cut(s)	53, 1106
BstAUI	TGTACA	2 cut(s)	1269, 1642
BstBAI	YACGTR	1 cut(s)	2735
BstBI	TTCGAA	1 cut(s)	1368
BstC8I	GCNNGC	4 cut(s)	75, 600, 2328, 3657
BstDSI	CCRYGG	1 cut(s)	60
BstEII	GGTNACC	1 cut(s)	3665
BstENI	CCTNNNNNAGG	3 cut(s)	711, 3030, 3588
BstF5I	GGATG	7 cut(s)	256, 490, 1436, 1539, 2089, 2962, 3007
BstFNI	CGCG	3 cut(s)	469, 477, 3659
BstH2I	RGCGCY	3 cut(s)	179, 630, 814
BstHHI	GCGC	5 cut(s)	178, 629, 813, 1860, 2471
BstMAI	GTCTC	5 cut(s)	1510, 2165, 2256, 2265, 3003
BstMCI	CGRYCG	3 cut(s)	1527, 2218, 3663
BstMWI	GCNNNNNNNGC	8 cut(s)	504, 659, 703, 751, 902, 982, 1974, 2466
BstNI	CCWGG	6 cut(s)	409, 1302, 1722, 2115, 2306, 2417
BstNSI	RCATGY	5 cut(s)	93, 1338, 1342, 3331, 3599
BstPI	GGTNACC	1 cut(s)	3665
BstSCI	CCNGG	7 cut(s)	407, 1300, 1720, 2113, 2304, 2415, 3286
BstSFI	CTRYAG	6 cut(s)	1250, 1482, 1792, 1978, 2464, 3564
BstSLI	GKGCMC	3 cut(s)	898, 1133, 3583
BstUI	CGCG	3 cut(s)	469, 477, 3659
BstV1I	GCAGC	6 cut(s)	917, 1678, 1806, 2662, 2971, 3586
BstV2I	GAAGAC	2 cut(s)	1357, 2631
BstX2I	RGATCY	2 cut(s)	353, 3630
BstXI	CCANNNNNNTGG	1 cut(s)	350
BstYI	RGATCY	2 cut(s)	353, 3630
BstZ17I	GTATAC	2 cut(s)	48, 3620
BstZI	CGGCCG	1 cut(s)	3660
BsuI	GTATCC	1 cut(s)	1839
BsuRI	GGCC	6 cut(s)	745, 935, 1065, 2602, 3477, 3662
BtgI	CCRYGG	1 cut(s)	60
BtsCI	GGATG	7 cut(s)	256, 490, 1436, 1539, 2089, 2962, 3007
BtsI	GCAGTG	4 cut(s)	1015, 1244, 3424, 3479
BtsIMutI	CAGTG	8 cut(s)	381, 1015, 1244, 1890, 2336, 3424, 3479, 3685
BveI	ACCTGC	2 cut(s)	1792, 3725
Cac8I	GCNNGC	4 cut(s)	75, 600, 2328, 3657
CciI	TCATGA	2 cut(s)	2428, 2659
CfoI	GCGC	5 cut(s)	178, 629, 813, 1860, 2471
Cfr10I	RCCGGY	3 cut(s)	73, 2213, 3662
Cfr13I	GGNCC	6 cut(s)	339, 682, 2601, 2843, 3476, 3611
CsiI	ACCWGGT	1 cut(s)	2415
Csp6I	GTAC	4 cut(s)	1270, 1643, 1741, 3413
CspAI	ACCGGT	1 cut(s)	2213
CviQI	GTAC	4 cut(s)	1270, 1643, 1741, 3413
DrdI	GACNNNNNNGTC	1 cut(s)	3017
DseDI	GACNNNNNNGTC	1 cut(s)	3017
EaeI	YGGCCR	1 cut(s)	3660
EagI	CGGCCG	1 cut(s)	3660
Eam1104I	CTCTTC	1 cut(s)	1815
EarI	CTCTTC	1 cut(s)	1815
Ecl136II	GAGCTC	1 cut(s)	379
EclXI	CGGCCG	1 cut(s)	3660
Eco130I	CCWWGG	5 cut(s)	60, 712, 2192, 2802, 3419
Eco147I	AGGCCT	1 cut(s)	745
Eco24I	GRGCYC	4 cut(s)	381, 2462, 2802, 3657
Eco31I	GGTCTC	1 cut(s)	3003
Eco32I	GATATC	3 cut(s)	835, 1397, 3343
Eco47I	GGWCC	4 cut(s)	339, 682, 2843, 3611
Eco47III	AGCGCT	1 cut(s)	628
Eco52I	CGGCCG	1 cut(s)	3660
Eco53kI	GAGCTC	1 cut(s)	379
Eco57I	CTGAAG	3 cut(s)	2342, 2652, 2904
Eco88I	CYCGRG	2 cut(s)	357, 2455
Eco91I	GGTNACC	1 cut(s)	3665
EcoICRI	GAGCTC	1 cut(s)	379
EcoNI	CCTNNNNNAGG	3 cut(s)	711, 3030, 3588
EcoO109I	RGGNCCY	1 cut(s)	682
EcoO65I	GGTNACC	1 cut(s)	3665
EcoRI	GAATTC	3 cut(s)	1370, 1570, 2450
EcoRII	CCWGG	6 cut(s)	407, 1300, 1720, 2113, 2304, 2415
EcoRV	GATATC	3 cut(s)	835, 1397, 3343
EcoT14I	CCWWGG	5 cut(s)	60, 712, 2192, 2802, 3419
EcoT22I	ATGCAT	3 cut(s)	1340, 1997, 3684
EcoT38I	GRGCYC	4 cut(s)	381, 2462, 2802, 3657
ErhI	CCWWGG	5 cut(s)	60, 712, 2192, 2802, 3419
FalI	AAGNNNNNCTT	6 cut(s)	979, 1011, 1794, 1826, 3617, 3649
FaqI	GGGAC	1 cut(s)	2534
FauNDI	CATATG	3 cut(s)	1626, 1964, 2230
FbaI	TGATCA	1 cut(s)	3084
FblI	GTMKAC	6 cut(s)	47, 1221, 1331, 1906, 3619, 3756
Fnu4HI	GCNGC	7 cut(s)	906, 1667, 1795, 2676, 2985, 3600, 3660
FokI	GGATG	7 cut(s)	263, 497, 1443, 1546, 2096, 2949, 3014
FriOI	GRGCYC	4 cut(s)	381, 2462, 2802, 3657
Fsp4HI	GCNGC	7 cut(s)	906, 1667, 1795, 2676, 2985, 3600, 3660
GlaI	GCGC	5 cut(s)	177, 628, 812, 1859, 2470
GluI	GCNGC	7 cut(s)	906, 1667, 1795, 2676, 2985, 3600, 3660
GsaI	CCCAGC	1 cut(s)	785
GsuI	CTGGAG	1 cut(s)	405
HaeII	RGCGCY	3 cut(s)	179, 630, 814
HaeIII	GGCC	6 cut(s)	745, 935, 1065, 2602, 3477, 3662
HapII	CCGG	8 cut(s)	74, 1575, 2214, 2846, 3080, 3246, 3288, 3663
HhaI	GCGC	5 cut(s)	178, 629, 813, 1860, 2471
Hin6I	GCGC	5 cut(s)	176, 627, 811, 1858, 2469
HinP1I	GCGC	5 cut(s)	176, 627, 811, 1858, 2469
HincII	GTYRAC	3 cut(s)	1907, 3435, 3757
HindII	GTYRAC	3 cut(s)	1907, 3435, 3757
HindIII	AAGCTT	4 cut(s)	993, 1871, 2612, 2837
HpaII	CCGG	8 cut(s)	74, 1575, 2214, 2846, 3080, 3246, 3288, 3663
HphI	GGTGA	6 cut(s)	1124, 1940, 2140, 2203, 2344, 3094
Hpy99I	CGWCG	3 cut(s)	3755, 3758, 3761
HpyAV	CCTTC	3 cut(s)	319, 824, 3702
HpyCH4IV	ACGT	4 cut(s)	2734, 2821, 2994, 3350
HpyF10VI	GCNNNNNNNGC	8 cut(s)	504, 659, 703, 751, 902, 982, 1974, 2466
HpySE526I	ACGT	4 cut(s)	2734, 2821, 2994, 3350
HspAI	GCGC	5 cut(s)	176, 627, 811, 1858, 2469
Kpn2I	TCCGGA	2 cut(s)	1574, 3245
KroI	GCCGGC	1 cut(s)	73
KroNI	GCCGGC	1 cut(s)	75
Ksp22I	TGATCA	1 cut(s)	3084
LguI	GCTCTTC	1 cut(s)	1815
LmnI	GCTCC	7 cut(s)	82, 376, 560, 1471, 2033, 3257, 3305
Lsp1109I	GCAGC	6 cut(s)	917, 1678, 1806, 2662, 2971, 3586
LweI	GCATC	7 cut(s)	448, 1678, 1795, 1982, 2037, 3691, 3721
MabI	ACCWGGT	1 cut(s)	2415
MaeII	ACGT	4 cut(s)	2734, 2821, 2994, 3350
MbiI	CCGCTC	1 cut(s)	866
MfeI	CAATTG	2 cut(s)	1451, 3408
MflI	RGATCY	2 cut(s)	353, 3630
MlyI	GAGTC	4 cut(s)	1227, 2258, 2621, 3122
MmeI	TCCRAC	3 cut(s)	2990, 3424, 3561
Mph1103I	ATGCAT	3 cut(s)	1340, 1997, 3684
MroI	TCCGGA	2 cut(s)	1574, 3245
MroNI	GCCGGC	1 cut(s)	73
MroXI	GAANNNNTTC	2 cut(s)	132, 3468
MslI	CAYNNNNRTG	7 cut(s)	153, 167, 1003, 1059, 1410, 2664, 2733
MspA1I	CMGCKG	1 cut(s)	2984
MspCI	CTTAAG	2 cut(s)	53, 1106
MspI	CCGG	8 cut(s)	74, 1575, 2214, 2846, 3080, 3246, 3288, 3663
MspR9I	CCNGG	7 cut(s)	409, 1302, 1722, 2115, 2306, 2417, 3288
MunI	CAATTG	2 cut(s)	1451, 3408
Mva1269I	GAATGC	3 cut(s)	134, 2306, 3283
MvaI	CCWGG	6 cut(s)	409, 1302, 1722, 2115, 2306, 2417
MvnI	CGCG	3 cut(s)	469, 477, 3659
MwoI	GCNNNNNNNGC	8 cut(s)	504, 659, 703, 751, 902, 982, 1974, 2466
NaeI	GCCGGC	1 cut(s)	75
NciI	CCSGG	1 cut(s)	3288
NcoI	CCATGG	1 cut(s)	60
NdeI	CATATG	3 cut(s)	1626, 1964, 2230
NgoMIV	GCCGGC	1 cut(s)	73
NheI	GCTAGC	1 cut(s)	2326
NlaIV	GGNNCC	7 cut(s)	78, 341, 705, 2035, 2237, 3307, 3580
NmuCI	GTSAC	7 cut(s)	1010, 1928, 2128, 2209, 2332, 2704, 2995
NsiI	ATGCAT	3 cut(s)	1340, 1997, 3684
NspI	RCATGY	5 cut(s)	93, 1338, 1342, 3331, 3599
NspV	TTCGAA	1 cut(s)	1368
OliI	CACNNNNGTG	2 cut(s)	1059, 2733
PaeR7I	CTCGAG	1 cut(s)	2455
PagI	TCATGA	2 cut(s)	2428, 2659
PaqCI	CACCTGC	1 cut(s)	1792
PceI	AGGCCT	1 cut(s)	745
PciSI	GCTCTTC	1 cut(s)	1815
PcsI	WCGNNNNNNNCGW	1 cut(s)	3762
PctI	GAATGC	3 cut(s)	134, 2306, 3283
PdiI	GCCGGC	1 cut(s)	75
PdmI	GAANNNNTTC	2 cut(s)	132, 3468
PfeI	GAWTC	7 cut(s)	41, 237, 395, 1155, 1417, 1445, 3358
PflMI	CCANNNNNTGG	1 cut(s)	2120
PfoI	TCCNGGA	1 cut(s)	3286
PinAI	ACCGGT	1 cut(s)	2213
PkrI	GCNGC	7 cut(s)	907, 1668, 1796, 2677, 2986, 3601, 3661
Ple19I	CGATCG	1 cut(s)	1527
PleI	GAGTC	4 cut(s)	1226, 2257, 2621, 3121
PpsI	GAGTC	4 cut(s)	1226, 2257, 2621, 3121
Ppu21I	YACGTR	1 cut(s)	2735
PpuMI	RGGWCCY	1 cut(s)	682
Psp124BI	GAGCTC	1 cut(s)	381
Psp5II	RGGWCCY	1 cut(s)	682
Psp6I	CCWGG	6 cut(s)	407, 1300, 1720, 2113, 2304, 2415
PspEI	GGTNACC	1 cut(s)	3665
PspFI	CCCAGC	1 cut(s)	781
PspGI	CCWGG	6 cut(s)	407, 1300, 1720, 2113, 2304, 2415
PspN4I	GGNNCC	7 cut(s)	78, 341, 705, 2035, 2237, 3307, 3580
PspPI	GGNCC	6 cut(s)	339, 682, 2601, 2843, 3476, 3611
PspPPI	RGGWCCY	1 cut(s)	682
PstI	CTGCAG	3 cut(s)	1486, 1796, 3568
PsuI	RGATCY	2 cut(s)	353, 3630
PvuI	CGATCG	1 cut(s)	1527
PvuII	CAGCTG	1 cut(s)	2984
RsaI	GTAC	4 cut(s)	1271, 1644, 1742, 3414
RsaNI	GTAC	4 cut(s)	1270, 1643, 1741, 3413
RseI	CAYNNNNRTG	7 cut(s)	153, 167, 1003, 1059, 1410, 2664, 2733
SacI	GAGCTC	1 cut(s)	381
SalI	GTCGAC	2 cut(s)	1905, 3755
SapI	GCTCTTC	1 cut(s)	1815
SatI	GCNGC	7 cut(s)	906, 1667, 1795, 2676, 2985, 3600, 3660
Sau96I	GGNCC	6 cut(s)	339, 682, 2601, 2843, 3476, 3611
ScaI	AGTACT	1 cut(s)	1742
SchI	GAGTC	4 cut(s)	1227, 2258, 2621, 3122
ScrFI	CCNGG	7 cut(s)	409, 1302, 1722, 2115, 2306, 2417, 3288
SexAI	ACCWGGT	1 cut(s)	2415
SfaNI	GCATC	7 cut(s)	448, 1678, 1795, 1982, 2037, 3691, 3721
SfcI	CTRYAG	6 cut(s)	1250, 1482, 1792, 1978, 2464, 3564
Sfr274I	CTCGAG	1 cut(s)	2455
SfuI	TTCGAA	1 cut(s)	1368
SgrDI	CGTCGACG	1 cut(s)	3755
SinI	GGWCC	4 cut(s)	339, 682, 2843, 3611
SlaI	CTCGAG	1 cut(s)	2455
SmiMI	CAYNNNNRTG	7 cut(s)	153, 167, 1003, 1059, 1410, 2664, 2733
SmlI	CTYRAG	6 cut(s)	53, 698, 1073, 1106, 2455, 3623
SmoI	CTYRAG	6 cut(s)	53, 698, 1073, 1106, 2455, 3623
SseBI	AGGCCT	1 cut(s)	745
SsiI	CCGC	5 cut(s)	469, 689, 864, 2330, 3659
SspI	AATATT	5 cut(s)	676, 848, 2006, 3045, 3273
SstI	GAGCTC	1 cut(s)	381
StuI	AGGCCT	1 cut(s)	745
StyD4I	CCNGG	7 cut(s)	407, 1300, 1720, 2113, 2304, 2415, 3286
StyI	CCWWGG	5 cut(s)	60, 712, 2192, 2802, 3419
TaiI	ACGT	4 cut(s)	2737, 2824, 2997, 3353
TaqII	GACCGA	1 cut(s)	1251
TatI	WGTACW	3 cut(s)	1269, 1642, 1740
TauI	GCSGC	1 cut(s)	3662
TfiI	GAWTC	7 cut(s)	41, 237, 395, 1155, 1417, 1445, 3358
TscAI	CASTG	8 cut(s)	388, 1015, 1251, 1897, 2343, 3431, 3486, 3692
TseFI	GTSAC	7 cut(s)	1010, 1928, 2128, 2209, 2332, 2704, 2995
TseI	GCWGC	6 cut(s)	905, 1666, 1794, 2675, 2984, 3599
Tsp45I	GTSAC	7 cut(s)	1010, 1928, 2128, 2209, 2332, 2704, 2995
TspGWI	ACGGA	3 cut(s)	1167, 1484, 3014
TspRI	CASTG	8 cut(s)	388, 1015, 1251, 1897, 2343, 3431, 3486, 3692
Van91I	CCANNNNNTGG	1 cut(s)	2120
Vha464I	CTTAAG	2 cut(s)	53, 1106
VneI	GTGCAC	2 cut(s)	894, 1129
VpaK11BI	GGWCC	4 cut(s)	339, 682, 2843, 3611
XagI	CCTNNNNNAGG	3 cut(s)	711, 3030, 3588
XbaI	TCTAGA	1 cut(s)	2617
XceI	RCATGY	5 cut(s)	93, 1338, 1342, 3331, 3599
XhoI	CTCGAG	1 cut(s)	2455
XmaJI	CCTAGG	1 cut(s)	2192
XmiI	GTMKAC	6 cut(s)	47, 1221, 1331, 1906, 3619, 3756
XmnI	GAANNNNTTC	2 cut(s)	132, 3468
ZrmI	AGTACT	1 cut(s)	1742
Zsp2I	ATGCAT	3 cut(s)	1340, 1997, 3684

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.