Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0002006

Physical Location & Seq

Genomic Coordinates Reverse (-)

81151962 .. 81161296

Gene Structure 9335 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000022126

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3804 bp

ATGAGGAGATGTGTAGTGCCTCCAAGGGAAAGGAGTCCAGACCTCTCTACTGCGACCCGAACCTGTTCACCAAAACCGACCTCTGTATCGCTCTTTCACTCTTCAAGCGCGCACGCGCTTAGATTATTCCGATCGATTTTCCGATCGACGACCGGTACCGGAATTTCATCTTCAATCCAGGAAACGGGTTCAAGCATGGATTCGTATACACTTAAGTCCATGGCAGACTTGCCGGCTCCTTTTCGAGCATGTTTTAATTTTAGGTATTTCAACTCTTTGCAGAGTGAATGCTTTCCTGCTTGTTTCCACTCAGATGTGAACATGGTTGTGTCAGCGCCAACTGGAAGTGGTAAAACAGTCATTTTTGAGCTTTGCATTTTGAGGCTTCTTTCAAGAGTCATCTCTGAGGATGGGAGGTTTACGCCTACAAATGGAACTCTCAAAACAATCTATATTGCCCCATCAAAGGCTTTAGTTCAGGAGAAGGTTCGTGATTGGACCCAGAAGTTTGGATCTCGGGGCATAGTTTGCTTGGAGCTCACCGGAGATAATGAATCTTACAACACCAGGAGTTTACAGGAAGCAGACATTATTTTAACCACTCCTGAGAAATTTGATGCTGTAACGCGGTATCGTATAAAGGATGGTGGCTTGGGCTTTTTCAGCGATATAGCACTTCTACTTATTGATGAAGTTCATCTGTTGAATGATCCTCGTGGAGCAGTTTTGGAAGCAATAGTTAGCAGAATAAAAATGCTTGCTTACAACCCTGAAATGAAGTCAAGCGCTCTGGCTCATGTTCGTTTTCTAGCTGTGTCTGCCACCATTCCAAATATTGAGGACCTTGCGGAGTGGCTCAAGGTGCCTGTCCAAGGGATTAAAAGGTTTGGAGAAGAAATGAGGCCTGTAAAGCTGACTACAAAAGTTTTTGGCTACGCCCCAGCAAAAAATGATTTTCTATTTGAAAAGCGCCTTCAAAACTACATTTTCGATATCCTTATGCAATATTCAAGAGGGAAGTCCGCTCTAATTTTTTGCTCAACTAGGAAAGGTGCACAAGAAGCAGCACAGATACTTTCTCAGACAGTCATGACCTTTGGTCATTCAAATCCATTCATTAAAAGCAGAGAACAGCAAGAGAGGCTAAGGGAAGCTTCATTGTCATGCAGTGACAGACAAATGCAAACTTATATGATTTATGGTGTTGGTTATCACAACGGCGGCCTTTGCCTCAAGGATCGTAATCTCATTGAAAGTCTTTTCCTTAAGGGTGATGTTCAAATTCTGTGCACAACAAATACTCTTGCTCACGGAATCAACCTACCAGCACATACAGTAGTCATAAAATCAACACAGCACTTCAACAAAGAAAAAGGAGTCTACATGGAAATTGACCGATCTACACTGCTACAGATGTGTGGGAGGGCAGGACGGCCACCTTTTGATGATACAGGAATGGTTATAATCATGACAAGAAGAGATACGGTTCACTTGTATGAGAATCTCTTGAATGGATGTGAGATGGTGGAATCACAATTGCTTTCATGTTTGACGGAGCATTTAACTGCGGAGATAGTTCAACTGACTGTCTCTGACATTACAAGGGCAATCGAATGGATGAAGTGCTCTTTCTTGTATGTGAGAATGAAGAAGAATCCGGGACACTATGCAGTTAGAAAAGTGATTTCCAATGATCATATAGAAAAGCATATGCTAGAGGTCTGTGTACAGAAAGTTAATGAGTTGTCGCAGCATCAGATGATATGGACCGATGAAGATGGTTTTCTGTTGAAACCACTAGAACCTGGGAGGCTGATGACGAAGTACTATTTGAAATTTGACACAATGAAATACATTATTCAGACACCTGCAAACTGCAGCTTGGAAGATGCTCTTCATGTGATATGCCGTGCTGAGGAAATTTCATGGATACAGCTCAGGCGCAATGAGAAAAAGCTTCTAAATGACATAAACACTGATAAAGATGGTCGACTTCGCTTTCACATTCTTGGTGACAAAGGGAAGCGAAAAAAGCGTATTCAAACCAGAGAAGAAAAGATATTTCTTTTGGCTAATGACTGCTTGACTGGTGATCCTTTGGTTCATGATTTATCCCTTACCCAGGATATGAACTCCATATGCTCAAATGGCTGTAGAATTGCCAGATGCATGAAAGAATTTTTTATTTACCAAAAGAATTACAAAGGAGCCTTGAACTCGATGCTTTTGGCAAAGTCTATGTATCAGAAACTCTGGGATGACAGTCCATACTTGCTGAAACAATTACCAGGGATTGGAATGGTGACTGCAAAGGCACTACATTCGATGGGAGTTAAATCATTTGAGACACTAGCTGAGGCTGATCCTAGGAGAATTGAGATAGTCACTGGTCGAAAATACCCATATGGGAACCATATAAAGGAGTCTCTACTGTCTCTACCTCCAAAGGTTGAGTTGAAAGTTGAGGAAACTGAATGCCGGGGGCAAGGGAAACTGAAGCTAGCGGTGACACTGACTAGGCTATCACAGACACTCCAATCAACAAAACGACATTATGCTGATATGATCGTAGGTTCAGAAGAAGATAACCTGGTTCTCTTTCATGAGAAAATAAGAGTTGATGAATTCTCGAGCCCCTATAGCGCAACAGTTCTCCTGTCAAATCCCCAACAAGGAAAGCTGACTGTTAAGGCTGATCTTATATTTGAAGAATACATTGGTATTGATCTAAACCAGGAGTTTATCTTAATGAAGAAAAGCAATTTAAGTGCAAATAACAAACGAGGAAAAAAAGCACCCTCTCTTCCTCTACCTGAGGAAGTATGTATCATAGAAGATGACACTTATGAAGGCCCTAGTGAAGTGCTTCCAAACTCGACGAAATCCAAAAGGGTAGATGGTCCCATGCCCAGTTTCAAGCTTCTAGATGAAGACTCTGAAGAAGATGAGGTTGCCAACAAAGTTCATGAAGATGGGATTGCTGCCAAAGTTCAAACCGATGACAAAGTGGTGACACAACAAACAGTATTTGACCACATACGTGAAAAGGCAAAGAACTTCCCTGTCTTGGTGTCATCAAATAGTGTGCTTCCTCCATCATCAGAGCCCTTGGAATTGGCAAGGAAACGTGCTCGTGAGAAGAAGCTTGGACCGGAAAGTGGAGTAGAGGTTCTGGAAGAATTAGAATGGAATAAAGTTCAAAGACGAACTGTGGTCAATCCATCTTCAGAACTTAAAGAAGCAGAACATCTTTCTAGTATCAAAAATCATCCAACTCCTAGAAGCTCAGCTGCCTTGAACGTCATGGATGGCAGAGGAGAAACAATATTTGAGCACATACGCACAAAAGCTAAGAATTTTCCGGTGATCAATAAACCAAATGTGGAGGAGGCTGAGTCTTTAATCCAGACAAAACAACGCCTATATAAGACTGAACCAGAGTTTAGCATGGATATGACTGCCGTGCTGAAAGGAAGAAAACCAAATGAGGTTATCAGAGATACTGAATGTGATAGGAGGTTGCTATCCAGAAGCTACTGCAACCCTTATGGAATATTGAATACTTCTCCCGGAGGTTTAAGCAATGGAGCCAATCTCTCATCAAGTAACATGCCATCTTTCGATATCTCCATGTTCAAGAATCCAAAGCGACCTGCAGATCCAGCAAGCACTGTGGAGAATACTAGGAAGAAACATCAACACTCTCCAATTGTACCACCAAGGCACTGCTCGTTGACAGCAGTTGGAAGATCCAGAGAAGTAGAAACGTTTCTTGGGTTTGAGAGTGTCTTTTCCTTTCTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000280 GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005730 GO:0005737 GO:0006139 GO:0006259 GO:0006310 GO:0006725 GO:0006807 GO:0006996 GO:0007049 GO:0007127 GO:0007131 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010501 GO:0016043 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022402 GO:0022414 GO:0031974 GO:0031981 GO:0034641 GO:0035825 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0048285 GO:0051321 GO:0061982 GO:0070013 GO:0070035 GO:0071704 GO:0071840 GO:0090304 GO:0140013 GO:0140098 GO:1901360 GO:1903046

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD HHH_5 Helicase_C PPR Sec63

Protein Analysis

1268

Amino Acids

143.13

Weight (kDa)

8.71

Isoelectric Point (pI)

46.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	92 - 281	2.1e-29	DEAD/DEAH box helicase
ResIII	PF04851	103 - 238	4e-08	Type III restriction enzyme, res subunit
Helicase_C	PF00271	399 - 478	2.7e-08	Helicase conserved C-terminal domain
WHD_SNRNP200	PF23445	510 - 589	1.8e-13	Winged Helix-turn-helix domain
Sec63	PF02889	599 - 914	1.5e-46	Sec63 Brl domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0002006)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G27730 AT3G27730 AT3G27730 AT3G27730 AT3G27730 AT3G27730 AT3G27730
fragaria_vesca	FvH4_6g51100
malus_domestica	MD09G1029000.v1.1 MD09G1029100.v1.1
prunus_persica	Prupe.3G288900_v2.0.a1
pyrus_communis	pycom111g02300 pycom111g02310
rosa_chinensis	RchiOBHm_Chr2g0089321 RchiOBHm_Chr2g0172661 RchiOBHm_Chr2g0172671
rosa_laevigata	RLG00000022075 RLG00000022122 RLG00000022126
rosa_multiflora	Rmu_sc0002053.1_g000008
rosa_roxburghii	Rroxscaffold_2G00079680 Rroxscaffold_2G00080200 Rroxscaffold_2G00080420
rosa_rugosa	Rorug01G0489000 Rorug01G0489100 Rorug02G0246000 Rorug02G0560500.1
rosa_samantha	Rh2AG637300 Rh2AG637400 Rh2AG637500 Rh2AG637900 Rh2BG650700 Rh2BG650800 Rh2BG650900 Rh2BG651500 Rh2CG045200 Rh2CG045300 Rh2CG610500 Rh2CG617200 Rh2CG617600 Rh2DG658400 Rh2DG664700 Rh2DG665400
rosa_wichuraiana	Rw2G052240 Rw2G052720

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1463
AarI	CACCTGC	1 cut(s)	1876
Acc36I	ACCTGC	2 cut(s)	1876, 3661
Acc65I	GGTACC	1 cut(s)	155
AccB1I	GGYRCC	2 cut(s)	155, 862
AccB7I	CCANNNNNTGG	1 cut(s)	2294
AccBSI	CCGCTC	1 cut(s)	1025
AccI	GTMKAC	3 cut(s)	206, 1380, 1990
AccII	CGCG	3 cut(s)	110, 116, 628
AciI	CCGC	6 cut(s)	628, 848, 1023, 1221, 1568, 2504
AclI	AACGTT	1 cut(s)	3767
AclWI	GGATC	7 cut(s)	520, 704, 1245, 2087, 2357, 3653, 3744
AcoI	YGGCCR	1 cut(s)	1433
AcsI	RAATTY	8 cut(s)	162, 611, 1281, 1835, 1920, 2177, 2624, 3355
AcuI	CTGAAG	3 cut(s)	2516, 2958, 3210
AfaI	GTAC	4 cut(s)	157, 1728, 1826, 3714
AfeI	AGCGCT	1 cut(s)	787
AflII	CTTAAG	2 cut(s)	212, 1265
AgeI	ACCGGT	1 cut(s)	152
AhdI	GACNNNNNGTC	1 cut(s)	1098
AjnI	CCWGG	7 cut(s)	177, 566, 1804, 2121, 2287, 2589, 2733
AjuI	GAANNNNNNNTTGG	4 cut(s)	2700, 2732, 3756, 3788
AleI	CACNNNNGTG	1 cut(s)	3039
AloI	GAACNNNNNNTCC	2 cut(s)	3153, 3185
Alw21I	GWGCWC	6 cut(s)	540, 1057, 1292, 1628, 3133, 3336
Alw26I	GTCTC	4 cut(s)	1594, 2339, 2430, 2439
Alw44I	GTGCAC	2 cut(s)	1053, 1288
AlwI	GGATC	7 cut(s)	520, 704, 1245, 2087, 2357, 3653, 3744
Ama87I	CYCGRG	2 cut(s)	516, 2629
Aor51HI	AGCGCT	1 cut(s)	787
AoxI	GGCC	4 cut(s)	902, 1222, 1433, 2851
ApaLI	GTGCAC	2 cut(s)	1053, 1288
ApeKI	GCWGC	5 cut(s)	1064, 1750, 1878, 2981, 3290
ApoI	RAATTY	8 cut(s)	162, 611, 1281, 1835, 1920, 2177, 2624, 3355
AsiGI	ACCGGT	1 cut(s)	152
Asp700I	GAANNNNTTC	4 cut(s)	64, 291, 2865, 3768
Asp718I	GGTACC	1 cut(s)	155
AspA2I	CCTAGG	1 cut(s)	2366
AspLEI	GCGC	8 cut(s)	110, 112, 118, 337, 788, 972, 1944, 2645
AspS9I	GGNCC	6 cut(s)	498, 841, 1767, 2852, 2900, 3149
AsuC2I	CCSGG	3 cut(s)	1659, 2480, 3570
AsuHPI	GGTGA	9 cut(s)	60, 532, 1283, 2024, 2102, 2314, 2518, 3022, 3376
AsuNHI	GCTAGC	1 cut(s)	2500
AvaI	CYCGRG	2 cut(s)	516, 2629
AvaII	GGWCC	5 cut(s)	498, 841, 1767, 2900, 3149
AvrII	CCTAGG	1 cut(s)	2366
AxyI	CCTNAGG	1 cut(s)	2814
BaeGI	GKGCMC	2 cut(s)	1057, 1292
BaeI	ACNNNNGTAYC	2 cut(s)	69, 102
BanI	GGYRCC	2 cut(s)	155, 862
BanII	GRGCYC	3 cut(s)	540, 2636, 3108
BauI	CACGAG	2 cut(s)	714, 3132
BbsI	GAAGAC	1 cut(s)	2937
Bbv12I	GWGCWC	6 cut(s)	540, 1057, 1292, 1628, 3133, 3336
BbvCI	CCTCAGC	2 cut(s)	1914, 2355
BbvI	GCAGC	5 cut(s)	1076, 1762, 1890, 2968, 3277
BceAI	ACGGC	4 cut(s)	1234, 1448, 1893, 3446
BcgI	CGANNNNNNTGC	2 cut(s)	2304, 2338
BciT130I	CCWGG	7 cut(s)	179, 568, 1806, 2123, 2289, 2591, 2735
BciVI	GTATCC	1 cut(s)	1923
BclI	TGATCA	2 cut(s)	1693, 3366
BcnI	CCSGG	3 cut(s)	1659, 2480, 3570
BcoDI	GTCTC	4 cut(s)	1594, 2339, 2430, 2439
BfmI	CTRYAG	5 cut(s)	1409, 1876, 2152, 2638, 3654
BfoI	RGCGCY	3 cut(s)	338, 789, 973
BfrI	CTTAAG	2 cut(s)	212, 1265
BfuAI	ACCTGC	2 cut(s)	1876, 3661
BfuI	GTATCC	1 cut(s)	1923
BisI	GCNGC	6 cut(s)	1065, 1222, 1751, 1879, 2982, 3291
BlnI	CCTAGG	1 cut(s)	2366
BlpI	GCTNAGC	1 cut(s)	3286
BlsI	GCNGC	6 cut(s)	1066, 1223, 1752, 1880, 2983, 3292
BmcAI	AGTACT	1 cut(s)	1826
Bme18I	GGWCC	5 cut(s)	498, 841, 1767, 2900, 3149
BmeRI	GACNNNNNGTC	1 cut(s)	1098
BmeT110I	CYCGRG	2 cut(s)	516, 2629
BmgT120I	GGNCC	6 cut(s)	498, 841, 1767, 2852, 2900, 3149
BmiI	GGNNCC	8 cut(s)	157, 237, 500, 864, 2209, 2411, 2902, 3589
BmrI	ACTGGG	1 cut(s)	2904
BmsI	GCATC	5 cut(s)	607, 1762, 1879, 2156, 2211
BmtI	GCTAGC	1 cut(s)	2504
BmuI	ACTGGG	1 cut(s)	2904
BpiI	GAAGAC	1 cut(s)	2937
BplI	GAGNNNNNCTC	2 cut(s)	3579, 3611
Bpu10I	CCTNAGC	4 cut(s)	1145, 1914, 1937, 2355
Bpu1102I	GCTNAGC	1 cut(s)	3286
BpuEI	CTTGAG	2 cut(s)	842, 1217
BpuMI	CCSGG	3 cut(s)	1659, 2480, 3570
Bsa29I	ATCGAT	1 cut(s)	134
BsaAI	YACGTR	1 cut(s)	3041
BsaBI	GATNNNNATC	1 cut(s)	1242
BsaWI	WCCGGW	5 cut(s)	152, 158, 542, 3151, 3361
BsaXI	ACNNNNNCTCC	6 cut(s)	2517, 2547, 3153, 3183, 3380, 3410
Bse118I	RCCGGY	2 cut(s)	152, 232
Bse1I	ACTGG	4 cut(s)	346, 2092, 2392, 2910
Bse21I	CCTNAGG	1 cut(s)	2814
Bse3DI	GCAATG	2 cut(s)	1951, 3589
Bse8I	GATNNNNATC	1 cut(s)	1242
BseBI	CCWGG	7 cut(s)	179, 568, 1806, 2123, 2289, 2591, 2735
BseCI	ATCGAT	1 cut(s)	134
BseGI	GGATG	7 cut(s)	415, 649, 1520, 1623, 2263, 3268, 3313
BseJI	GATNNNNATC	1 cut(s)	1242
BseMI	GCAATG	2 cut(s)	1951, 3589
BseNI	ACTGG	4 cut(s)	346, 2092, 2392, 2910
BsePI	GCGCGC	1 cut(s)	108
BseRI	GAGGAG	3 cut(s)	19, 3330, 3401
BseSI	GKGCMC	2 cut(s)	1057, 1292
BseXI	GCAGC	5 cut(s)	1076, 1762, 1890, 2968, 3277
BseYI	CCCAGC	1 cut(s)	940
Bsh1236I	CGCG	3 cut(s)	110, 116, 628
Bsh1285I	CGRYCG	3 cut(s)	134, 146, 153
BshFI	GGCC	4 cut(s)	904, 1224, 1435, 2853
BshNI	GGYRCC	2 cut(s)	155, 862
BshTI	ACCGGT	1 cut(s)	152
BshVI	ATCGAT	1 cut(s)	134
BsiEI	CGRYCG	3 cut(s)	134, 146, 153
BsiHKAI	GWGCWC	6 cut(s)	540, 1057, 1292, 1628, 3133, 3336
BsiHKCI	CYCGRG	2 cut(s)	516, 2629
BsiSI	CCGG	9 cut(s)	153, 159, 233, 543, 1658, 2479, 3152, 3362, 3570
BslFI	GGGAC	2 cut(s)	1674, 2886
BsmAI	GTCTC	4 cut(s)	1594, 2339, 2430, 2439
BsmFI	GGGAC	2 cut(s)	1674, 2886
BsmI	GAATGC	2 cut(s)	293, 2480
BsnI	GGCC	4 cut(s)	904, 1224, 1435, 2853
BsoBI	CYCGRG	2 cut(s)	516, 2629
Bsp1286I	GDGCHC	8 cut(s)	540, 1057, 1292, 1628, 2636, 3108, 3133, 3336
Bsp1407I	TGTACA	1 cut(s)	1726
Bsp1720I	GCTNAGC	1 cut(s)	3286
Bsp19I	CCATGG	1 cut(s)	219
BspACI	CCGC	6 cut(s)	628, 848, 1023, 1221, 1568, 2504
BspANI	GGCC	4 cut(s)	904, 1224, 1435, 2853
BspDI	ATCGAT	1 cut(s)	134
BspFNI	CGCG	3 cut(s)	110, 116, 628
BspHI	TCATGA	5 cut(s)	1089, 1467, 2104, 2602, 2965
BspLI	GGNNCC	8 cut(s)	157, 237, 500, 864, 2209, 2411, 2902, 3589
BspMAI	CTGCAG	2 cut(s)	1880, 3658
BspMI	ACCTGC	2 cut(s)	1876, 3661
BspOI	GCTAGC	1 cut(s)	2504
BspPI	GGATC	7 cut(s)	520, 704, 1245, 2087, 2357, 3653, 3744
BspQI	GCTCTTC	1 cut(s)	1899
BspT107I	GGYRCC	2 cut(s)	155, 862
BspTI	CTTAAG	2 cut(s)	212, 1265
BsrBI	CCGCTC	1 cut(s)	1025
BsrDI	GCAATG	2 cut(s)	1951, 3589
BsrFI	RCCGGY	2 cut(s)	152, 232
BsrGI	TGTACA	1 cut(s)	1726
BsrI	ACTGG	4 cut(s)	346, 2092, 2392, 2910
BssAI	RCCGGY	2 cut(s)	152, 232
BssHII	GCGCGC	1 cut(s)	108
BssNAI	GTATAC	1 cut(s)	207
BssSI	CACGAG	2 cut(s)	714, 3132
BssT1I	CCWWGG	6 cut(s)	23, 219, 871, 2366, 3108, 3719
Bst1107I	GTATAC	1 cut(s)	207
Bst2BI	CACGAG	2 cut(s)	714, 3132
Bst2UI	CCWGG	7 cut(s)	179, 568, 1806, 2123, 2289, 2591, 2735
Bst6I	CTCTTC	4 cut(s)	106, 1471, 1899, 2808
BstAFI	CTTAAG	2 cut(s)	212, 1265
BstAPI	GCANNNNNTGC	1 cut(s)	528
BstAUI	TGTACA	1 cut(s)	1726
BstBAI	YACGTR	1 cut(s)	3041
BstC8I	GCNNGC	6 cut(s)	110, 114, 234, 759, 2502, 3667
BstDSI	CCRYGG	1 cut(s)	219
BstENI	CCTNNNNNAGG	2 cut(s)	870, 2120
BstF5I	GGATG	7 cut(s)	415, 649, 1520, 1623, 2263, 3268, 3313
BstFNI	CGCG	3 cut(s)	110, 116, 628
BstH2I	RGCGCY	3 cut(s)	338, 789, 973
BstHHI	GCGC	8 cut(s)	110, 112, 118, 337, 788, 972, 1944, 2645
BstMAI	GTCTC	4 cut(s)	1594, 2339, 2430, 2439
BstMCI	CGRYCG	3 cut(s)	134, 146, 153
BstNI	CCWGG	7 cut(s)	179, 568, 1806, 2123, 2289, 2591, 2735
BstNSI	RCATGY	2 cut(s)	252, 3613
BstSFI	CTRYAG	5 cut(s)	1409, 1876, 2152, 2638, 3654
BstSLI	GKGCMC	2 cut(s)	1057, 1292
BstUI	CGCG	3 cut(s)	110, 116, 628
BstV1I	GCAGC	5 cut(s)	1076, 1762, 1890, 2968, 3277
BstV2I	GAAGAC	1 cut(s)	2937
BstX2I	RGATCY	3 cut(s)	512, 3658, 3749
BstXI	CCANNNNNNTGG	1 cut(s)	509
BstYI	RGATCY	3 cut(s)	512, 3658, 3749
BstZ17I	GTATAC	1 cut(s)	207
Bsu15I	ATCGAT	1 cut(s)	134
Bsu36I	CCTNAGG	1 cut(s)	2814
BsuI	GTATCC	1 cut(s)	1923
BsuRI	GGCC	4 cut(s)	904, 1224, 1435, 2853
BsuTUI	ATCGAT	1 cut(s)	134
BtgI	CCRYGG	1 cut(s)	219
BtsCI	GGATG	7 cut(s)	415, 649, 1520, 1623, 2263, 3268, 3313
BtsI	GCAGTG	3 cut(s)	1174, 1403, 3724
BtsIMutI	CAGTG	7 cut(s)	1174, 1403, 1974, 2385, 2510, 3669, 3724
BveI	ACCTGC	2 cut(s)	1876, 3661
Cac8I	GCNNGC	6 cut(s)	110, 114, 234, 759, 2502, 3667
CciI	TCATGA	5 cut(s)	1089, 1467, 2104, 2602, 2965
CfoI	GCGC	8 cut(s)	110, 112, 118, 337, 788, 972, 1944, 2645
Cfr10I	RCCGGY	2 cut(s)	152, 232
Cfr13I	GGNCC	6 cut(s)	498, 841, 1767, 2852, 2900, 3149
ClaI	ATCGAT	1 cut(s)	134
CsiI	ACCWGGT	1 cut(s)	2589
Csp6I	GTAC	4 cut(s)	156, 1727, 1825, 3713
CspAI	ACCGGT	1 cut(s)	152
CspCI	CAANNNNNGTGG	2 cut(s)	3654, 3689
CviQI	GTAC	4 cut(s)	156, 1727, 1825, 3713
DriI	GACNNNNNGTC	1 cut(s)	1098
EaeI	YGGCCR	1 cut(s)	1433
Eam1104I	CTCTTC	4 cut(s)	106, 1471, 1899, 2808
Eam1105I	GACNNNNNGTC	1 cut(s)	1098
EarI	CTCTTC	4 cut(s)	106, 1471, 1899, 2808
Ecl136II	GAGCTC	1 cut(s)	538
Eco130I	CCWWGG	6 cut(s)	23, 219, 871, 2366, 3108, 3719
Eco147I	AGGCCT	1 cut(s)	904
Eco24I	GRGCYC	3 cut(s)	540, 2636, 3108
Eco32I	GATATC	2 cut(s)	994, 3625
Eco47I	GGWCC	5 cut(s)	498, 841, 1767, 2900, 3149
Eco47III	AGCGCT	1 cut(s)	787
Eco53kI	GAGCTC	1 cut(s)	538
Eco57I	CTGAAG	3 cut(s)	2516, 2958, 3210
Eco81I	CCTNAGG	1 cut(s)	2814
Eco88I	CYCGRG	2 cut(s)	516, 2629
EcoICRI	GAGCTC	1 cut(s)	538
EcoNI	CCTNNNNNAGG	2 cut(s)	870, 2120
EcoO109I	RGGNCCY	2 cut(s)	841, 2852
EcoRI	GAATTC	1 cut(s)	2624
EcoRII	CCWGG	7 cut(s)	177, 566, 1804, 2121, 2287, 2589, 2733
EcoRV	GATATC	2 cut(s)	994, 3625
EcoT14I	CCWWGG	6 cut(s)	23, 219, 871, 2366, 3108, 3719
EcoT22I	ATGCAT	1 cut(s)	2171
EcoT38I	GRGCYC	3 cut(s)	540, 2636, 3108
ErhI	CCWWGG	6 cut(s)	23, 219, 871, 2366, 3108, 3719
FalI	AAGNNNNNCTT	4 cut(s)	1138, 1170, 1878, 1910
FaqI	GGGAC	2 cut(s)	1674, 2886
FauNDI	CATATG	3 cut(s)	1710, 2138, 2404
FbaI	TGATCA	2 cut(s)	1693, 3366
FblI	GTMKAC	3 cut(s)	206, 1380, 1990
Fnu4HI	GCNGC	6 cut(s)	1065, 1222, 1751, 1879, 2982, 3291
FokI	GGATG	7 cut(s)	422, 656, 1527, 1630, 2270, 3255, 3320
FriOI	GRGCYC	3 cut(s)	540, 2636, 3108
Fsp4HI	GCNGC	6 cut(s)	1065, 1222, 1751, 1879, 2982, 3291
GlaI	GCGC	8 cut(s)	109, 111, 117, 336, 787, 971, 1943, 2644
GluI	GCNGC	6 cut(s)	1065, 1222, 1751, 1879, 2982, 3291
GsaI	CCCAGC	1 cut(s)	944
HaeII	RGCGCY	3 cut(s)	338, 789, 973
HaeIII	GGCC	4 cut(s)	904, 1224, 1435, 2853
HapII	CCGG	9 cut(s)	153, 159, 233, 543, 1658, 2479, 3152, 3362, 3570
HhaI	GCGC	8 cut(s)	110, 112, 118, 337, 788, 972, 1944, 2645
Hin6I	GCGC	8 cut(s)	108, 110, 116, 335, 786, 970, 1942, 2643
HinP1I	GCGC	8 cut(s)	108, 110, 116, 335, 786, 970, 1942, 2643
HincII	GTYRAC	2 cut(s)	1991, 3735
HindII	GTYRAC	2 cut(s)	1991, 3735
HindIII	AAGCTT	4 cut(s)	1152, 1955, 2918, 3143
HpaII	CCGG	9 cut(s)	153, 159, 233, 543, 1658, 2479, 3152, 3362, 3570
HphI	GGTGA	9 cut(s)	60, 532, 1283, 2024, 2102, 2314, 2518, 3022, 3376
Hpy99I	CGWCG	2 cut(s)	151, 2881
HpyAV	CCTTC	3 cut(s)	478, 983, 2843
HpyCH4IV	ACGT	4 cut(s)	3040, 3127, 3300, 3767
HpySE526I	ACGT	4 cut(s)	3040, 3127, 3300, 3767
HspAI	GCGC	8 cut(s)	108, 110, 116, 335, 786, 970, 1942, 2643
KpnI	GGTACC	1 cut(s)	159
KroI	GCCGGC	1 cut(s)	232
KroNI	GCCGGC	1 cut(s)	234
Ksp22I	TGATCA	2 cut(s)	1693, 3366
LguI	GCTCTTC	1 cut(s)	1899
LmnI	GCTCC	6 cut(s)	241, 535, 719, 1555, 2207, 3587
Lsp1109I	GCAGC	5 cut(s)	1076, 1762, 1890, 2968, 3277
LweI	GCATC	5 cut(s)	607, 1762, 1879, 2156, 2211
MabI	ACCWGGT	1 cut(s)	2589
MaeII	ACGT	4 cut(s)	3040, 3127, 3300, 3767
MaeIII	GTNAC	8 cut(s)	622, 1169, 2012, 2302, 2383, 2506, 3010, 3605
MbiI	CCGCTC	1 cut(s)	1025
MfeI	CAATTG	2 cut(s)	1535, 3708
MflI	RGATCY	3 cut(s)	512, 3658, 3749
MhlI	GDGCHC	8 cut(s)	540, 1057, 1292, 1628, 2636, 3108, 3133, 3336
MlyI	GAGTC	6 cut(s)	43, 405, 1386, 2432, 2927, 3404
MmeI	TCCRAC	2 cut(s)	3296, 3724
Mph1103I	ATGCAT	1 cut(s)	2171
MroNI	GCCGGC	1 cut(s)	232
MroXI	GAANNNNTTC	4 cut(s)	64, 291, 2865, 3768
MslI	CAYNNNNRTG	7 cut(s)	312, 326, 1162, 1494, 2970, 3039, 3452
MspA1I	CMGCKG	1 cut(s)	3290
MspCI	CTTAAG	2 cut(s)	212, 1265
MspI	CCGG	9 cut(s)	153, 159, 233, 543, 1658, 2479, 3152, 3362, 3570
MunI	CAATTG	2 cut(s)	1535, 3708
Mva1269I	GAATGC	2 cut(s)	293, 2480
MvaI	CCWGG	7 cut(s)	179, 568, 1806, 2123, 2289, 2591, 2735
MvnI	CGCG	3 cut(s)	110, 116, 628
NaeI	GCCGGC	1 cut(s)	234
NciI	CCSGG	3 cut(s)	1659, 2480, 3570
NcoI	CCATGG	1 cut(s)	219
NdeI	CATATG	3 cut(s)	1710, 2138, 2404
NgoMIV	GCCGGC	1 cut(s)	232
NheI	GCTAGC	1 cut(s)	2500
NlaIV	GGNNCC	8 cut(s)	157, 237, 500, 864, 2209, 2411, 2902, 3589
NmuCI	GTSAC	6 cut(s)	1169, 2012, 2302, 2383, 2506, 3010
NsiI	ATGCAT	1 cut(s)	2171
NspI	RCATGY	2 cut(s)	252, 3613
OliI	CACNNNNGTG	1 cut(s)	3039
PaeR7I	CTCGAG	1 cut(s)	2629
PagI	TCATGA	5 cut(s)	1089, 1467, 2104, 2602, 2965
PaqCI	CACCTGC	1 cut(s)	1876
PauI	GCGCGC	1 cut(s)	108
PceI	AGGCCT	1 cut(s)	904
PciSI	GCTCTTC	1 cut(s)	1899
PctI	GAATGC	2 cut(s)	293, 2480
PdiI	GCCGGC	1 cut(s)	234
PdmI	GAANNNNTTC	4 cut(s)	64, 291, 2865, 3768
PfeI	GAWTC	7 cut(s)	200, 554, 1314, 1501, 1529, 1654, 3640
PflMI	CCANNNNNTGG	1 cut(s)	2294
PfoI	TCCNGGA	3 cut(s)	177, 1657, 3568
PinAI	ACCGGT	1 cut(s)	152
PkrI	GCNGC	6 cut(s)	1066, 1223, 1752, 1880, 2983, 3292
Ple19I	CGATCG	2 cut(s)	134, 146
PleI	GAGTC	6 cut(s)	42, 404, 1385, 2431, 2927, 3403
PpsI	GAGTC	6 cut(s)	42, 404, 1385, 2431, 2927, 3403
Ppu21I	YACGTR	1 cut(s)	3041
PpuMI	RGGWCCY	1 cut(s)	841
PsiI	TTATAA	1 cut(s)	1463
Psp124BI	GAGCTC	1 cut(s)	540
Psp1406I	AACGTT	1 cut(s)	3767
Psp5II	RGGWCCY	1 cut(s)	841
Psp6I	CCWGG	7 cut(s)	177, 566, 1804, 2121, 2287, 2589, 2733
PspFI	CCCAGC	1 cut(s)	940
PspGI	CCWGG	7 cut(s)	177, 566, 1804, 2121, 2287, 2589, 2733
PspN4I	GGNNCC	8 cut(s)	157, 237, 500, 864, 2209, 2411, 2902, 3589
PspPI	GGNCC	6 cut(s)	498, 841, 1767, 2852, 2900, 3149
PspPPI	RGGWCCY	1 cut(s)	841
PstI	CTGCAG	2 cut(s)	1880, 3658
PsuI	RGATCY	3 cut(s)	512, 3658, 3749
PteI	GCGCGC	1 cut(s)	108
PvuI	CGATCG	2 cut(s)	134, 146
PvuII	CAGCTG	1 cut(s)	3290
RsaI	GTAC	4 cut(s)	157, 1728, 1826, 3714
RsaNI	GTAC	4 cut(s)	156, 1727, 1825, 3713
RseI	CAYNNNNRTG	7 cut(s)	312, 326, 1162, 1494, 2970, 3039, 3452
SacI	GAGCTC	1 cut(s)	540
SalI	GTCGAC	1 cut(s)	1989
SapI	GCTCTTC	1 cut(s)	1899
SatI	GCNGC	6 cut(s)	1065, 1222, 1751, 1879, 2982, 3291
Sau96I	GGNCC	6 cut(s)	498, 841, 1767, 2852, 2900, 3149
ScaI	AGTACT	1 cut(s)	1826
SchI	GAGTC	6 cut(s)	43, 405, 1386, 2432, 2927, 3404
SduI	GDGCHC	8 cut(s)	540, 1057, 1292, 1628, 2636, 3108, 3133, 3336
SexAI	ACCWGGT	1 cut(s)	2589
SfaNI	GCATC	5 cut(s)	607, 1762, 1879, 2156, 2211
SfcI	CTRYAG	5 cut(s)	1409, 1876, 2152, 2638, 3654
Sfr274I	CTCGAG	1 cut(s)	2629
SinI	GGWCC	5 cut(s)	498, 841, 1767, 2900, 3149
SlaI	CTCGAG	1 cut(s)	2629
SmiMI	CAYNNNNRTG	7 cut(s)	312, 326, 1162, 1494, 2970, 3039, 3452
SmlI	CTYRAG	5 cut(s)	212, 857, 1232, 1265, 2629
SmoI	CTYRAG	5 cut(s)	212, 857, 1232, 1265, 2629
SseBI	AGGCCT	1 cut(s)	904
SsiI	CCGC	6 cut(s)	628, 848, 1023, 1221, 1568, 2504
SspI	AATATT	4 cut(s)	835, 1007, 3327, 3555
SstI	GAGCTC	1 cut(s)	540
StuI	AGGCCT	1 cut(s)	904
StyI	CCWWGG	6 cut(s)	23, 219, 871, 2366, 3108, 3719
TaiI	ACGT	4 cut(s)	3043, 3130, 3303, 3770
TaqII	GACCGA	2 cut(s)	1410, 1784
TatI	WGTACW	2 cut(s)	1726, 1824
TauI	GCSGC	1 cut(s)	1224
TfiI	GAWTC	7 cut(s)	200, 554, 1314, 1501, 1529, 1654, 3640
TscAI	CASTG	7 cut(s)	1174, 1410, 1981, 2392, 2517, 3676, 3731
TseFI	GTSAC	6 cut(s)	1169, 2012, 2302, 2383, 2506, 3010
TseI	GCWGC	5 cut(s)	1064, 1750, 1878, 2981, 3290
Tsp45I	GTSAC	6 cut(s)	1169, 2012, 2302, 2383, 2506, 3010
TspGWI	ACGGA	2 cut(s)	1326, 1568
TspRI	CASTG	7 cut(s)	1174, 1410, 1981, 2392, 2517, 3676, 3731
Van91I	CCANNNNNTGG	1 cut(s)	2294
Vha464I	CTTAAG	2 cut(s)	212, 1265
VneI	GTGCAC	2 cut(s)	1053, 1288
VpaK11BI	GGWCC	5 cut(s)	498, 841, 1767, 2900, 3149
XagI	CCTNNNNNAGG	2 cut(s)	870, 2120
XapI	RAATTY	8 cut(s)	162, 611, 1281, 1835, 1920, 2177, 2624, 3355
XbaI	TCTAGA	1 cut(s)	2923
XceI	RCATGY	2 cut(s)	252, 3613
XhoI	CTCGAG	1 cut(s)	2629
XmaJI	CCTAGG	1 cut(s)	2366
XmiI	GTMKAC	3 cut(s)	206, 1380, 1990
XmnI	GAANNNNTTC	4 cut(s)	64, 291, 2865, 3768
ZrmI	AGTACT	1 cut(s)	1826
Zsp2I	ATGCAT	1 cut(s)	2171

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.