Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0002006

Physical Location & Seq

Genomic Coordinates Forward (+)

80097902 .. 80107141

Gene Structure 9240 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G052240.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3504 bp

ATGGATTTTTTTATCTTAATTCTTCATCCGAAACAGATATCCCAAATTAAAGGTCCTCATCGTCTTCCTTCACTTCGCACAGTCCAGACCTCTCTACTGCAGCCCCGACCTCTTCACCATAATCGACCTCTGTATCGTTCTTTCACTCTTCACGCGCACGCGCTTAGATTAAGCCGATCGGTTTTCAGATCGACGACCGGTACCGAAATTTCATCTTCAATCCAGGAAACGGGTTCAAGCATGGATTCGTATACACTTAAGTCCATGGCAGACTTGCCGGCTCCTTTTCGAGCATGTTTTAATTTTAGGTATTTCAACTCTTTGCAGAGTGAATGCTTTCCTGCTTGTTTCCACTCAGATGTGAACATGGTTGTGTCAGCGCCAACTGGAAGTGGTAAAACAGTCATTTTTGAGCTTTGCATTTTGAGGCTTCTCTCAAGATTCATCTCTGAGGATGGGAGGTTTACACCTACAAATGGAACTCTCAAAACAATCTATATTGCCCCATCAAAGGCTTTAGTTCAGGAGAAGGTTTGTGATTGTACCCAGAAGTTTGGATCTCGGGGCATAGTTTGCTTGGAGCTCACCGGAGATAATGAATCTTACAACACCAGGAGTTTACAGGAAGCAGACATTATTTTAACCACTCCTGAGAAATTTGATGCTGTAACGCGGTATCGCGTAAAGGATGGTGGATTGGGCTTTTTCAGCGATATAGAACTTCTACTTATTGATGAAGTTCATCTGTTGAATGATCCTTGTGGAGCAGTTTTGGAAGCAATAGTTAGCAGAATAAAAATGCTTGCTTACAACCCTGAAATGAAGTCAAGCGCTCTGGCTCATGTTCGTTTTCTAGCTATGTCTGCCACCATTCCAAATATTGAGGACCTTGCGGAGTGGCTCAAGGTGCCTGTCCAAGGGATTAAAAGGTTTGGAGAAGAAATGAGGCCTGTAAAGCTGACTACAAAACACCTTCAAAACTATATTTTCGATATCCTTATGCAATATTCAAGAGGGAAGTCCGCTCTAATTTTTTGCTTAACTAGAAAAGGTGCACAAGAAGCAGCACAGATACTTTCTCAGACAGTCATGGCCTTTGGTCATTCAAATCCATTCATTAAAAGCAGAGAACAGCAAGAGAGGCTAAGGGAAGCTTCATTGTCATGCAGTGACAAACAAATGCAATCTTATATGATTTATGGTGTTGGTTATCACAACGGTGGCCTTTGCCTCAAGGATCGTAATCTCATTGAAAGTCTTTTCCTTAAGGGTGATGTTCAAATTCCGTGCACAACAAATACTCTTGCTCATGGAATCAACCTACCAGCACATACAGTAGTCATAAAATCAACACAGCACTTCAACAAAGAAAAAGGAGTCTACATGGAAATTGACCGATCTACACTGCTACAGATGTGTGGGAGGGAAGGTCGCCCACCTTTTGATGATACAGGAATGGTTATAATCATGGCAAGAAGAGATACGGTTCACTTGTATGAGAATCTCTTGAATGGATGTGAGATGGTGGAATCACAATTGCTTTCATGTTTGACGGAGCATTTAACTGCAGAGATAGTTCAACTGACTGTCTCTGACATTACAAGGGCAATCGAATGGATGAAGTGCTCTTTCTTGTATGTGAGAATGAAGAAGAATCCCGGACACTATGCAGTTAGAAAAGTGATTTCCAATGATCGTATAGAAAAGCATATGCTAGAGGTCTGTGTACAGAAAGTTAATGAGTTGTCGCAGCATCAGATGATATGGACTGATGAAGATGGTTTTCTGTTGAAACCACTAGAACCTGGGAGGCTGATGACGAAGTACTACTTGAAATTTGACACAATGAAATACATTATTCAGACACCTGCAAACTGCAGCTTGGAAGATGCTCTTCATGTGATATGCCGTGCTGAGGAAATTTCATGGATACAGCTCAGGCGCAATGAGAAAAAGCTTCTAAATGACATAAACACTGATAAAGATGGTCGACTTCGCTTTCACATTCTTGGTGACAAAGGGAAGCGAAAAAAGCGTATTCAAACCAGAGAAGAAAAGATATTTCTTTTGGCTAATGACTGCTTGACTGGTGATCCATCGGTTCATGATTTATCCCTTACCCAGGATATGAACTCCATGTGCTCAAATGGCTGTAGAATTGCCAGATGCATGAAAGAATTTTTTATTTACCAAAAGAATTACAGAGGAGCCTTGAACTCGATGCTTTTGGCAAAGTCTTTGTATCAGAAACTCTGGGATGACAGTCCATACTTGCTGAAACAATTACCAGGGATTGGAATGGTGACTGCGAAGGCACTACATTCGATGGGAGTTAAATCATTTGAGACACTAGCTGAGGCTGATCCTAGGATAATTGAGATGGTCACTGGTCGAAAATACCCATATGGGAACCACATAAAAGAGTCTCTACTGAAACTGAATGCCCGGGGGCAAGGGAAACTGAAGCTAGCGGTGACACTGACTAGGCTATCACAGACACTCCAATCAACAAAACGACATTATGCTGATATGATTGTAGGTTCAGAAGAAGATAACCTGGTTCTCTTTCATGAGAAAATAAGGCCCAGTTTCAAGCTTCTAGATGAAGACTCTGAAGAAGATGAGGTTGCCGACAAAGTTCATGAAGATGAGATTGCTGCCAAATTTCAAACTGATGACAAAGTTGTGACACAACAAACAGTATTTGACCACATACGTGAAAAGGCAAAGAACTTCCCTGTCTTGGTGTCATCAAATAGTGTGCTTCCTCCATCATCAGAGCCCTTGGAATTAGCAAGGAAACGTGCTCGTGAGAAGAAGCTTGGACCGGAAAGTGGAGTAGAGGTTCTGGAAGAATTAGAATGGAATAAAGTTCAAAGACGAACTGTGGTCAATCCATCTTCAGAACTTAAAGAAGCAGAACAACATCTTTCTAGTATCAAAAATCATCCAAATCCCAGAAGCTCAGCTGCCCTGAACGTCATGGATGGCAGAGGAGAAACCATATTTGAGCACATACGCACAAAAGCTAAGAATTTTCCGGTGATCAATAAACCAAATGTGGAGGAGGCTGAGTCTTTAATCCAGACAAAACAACGCCTGTATAAGACTGAACCAGAGTTTAGCATGGATATTACTGCCGTGCTGAAAGGAAGAAAACCAAATGAGGTTATCAGAGATACTGAATGTGATAGGAGGTTGCTCTCCGGAAGCTCCTGCAACCCTCATGGAATATTGAATGCTTCTCCCGGAGGTTTAAGCAATGGAGCCAATCTCTCATCAAGCAACATGCCATCTTTCGATATCTCCATGTTCAAGAATCCGAAGCGACCTGCAGATCCAGCAAGCACTGTGGAGAATACTAGGAAGAAACATCAACACTCTCCAATTGTACCACCAAGGCACTGCTGGTTGACAGCAGTTGGAAAATCCAGAGAAGTAGAAACATTTCTTGGGTTTGAGAGTGTCTTTTCCTTTCATTGGCTAGTGTGTGGCGTGGGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000280 GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005730 GO:0005737 GO:0006139 GO:0006259 GO:0006310 GO:0006725 GO:0006807 GO:0006996 GO:0007049 GO:0007127 GO:0007131 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010501 GO:0016043 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022402 GO:0022414 GO:0031974 GO:0031981 GO:0034641 GO:0035825 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0048285 GO:0051321 GO:0061982 GO:0070013 GO:0070035 GO:0071704 GO:0071840 GO:0090304 GO:0140013 GO:0140098 GO:1901360 GO:1903046

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD HHH_5 Helicase_C PPR Sec63

Protein Analysis

1167

Amino Acids

132.24

Weight (kDa)

8.73

Isoelectric Point (pI)

46.23

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	107 - 296	9.5e-29	DEAD/DEAH box helicase
ResIII	PF04851	118 - 291	7.4e-08	Type III restriction enzyme, res subunit
WHD_SNRNP200	PF23445	510 - 589	5.8e-13	Winged Helix-turn-helix domain
Sec63	PF02889	599 - 861	7.4e-40	Sec63 Brl domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002006)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G27730 AT3G27730 AT3G27730 AT3G27730 AT3G27730 AT3G27730 AT3G27730
fragaria_vesca	FvH4_6g51100
malus_domestica	MD09G1029000.v1.1 MD09G1029100.v1.1
prunus_persica	Prupe.3G288900_v2.0.a1
pyrus_communis	pycom111g02300 pycom111g02310
rosa_chinensis	RchiOBHm_Chr2g0089321 RchiOBHm_Chr2g0172661 RchiOBHm_Chr2g0172671
rosa_laevigata	RLG00000022075 RLG00000022122 RLG00000022126
rosa_multiflora	Rmu_sc0002053.1_g000008
rosa_roxburghii	Rroxscaffold_2G00079680 Rroxscaffold_2G00080200 Rroxscaffold_2G00080420
rosa_rugosa	Rorug01G0489000 Rorug01G0489100 Rorug02G0246000 Rorug02G0560500.1
rosa_samantha	Rh2AG637300 Rh2AG637400 Rh2AG637500 Rh2AG637900 Rh2BG650700 Rh2BG650800 Rh2BG650900 Rh2BG651500 Rh2CG045200 Rh2CG045300 Rh2CG610500 Rh2CG617200 Rh2CG617600 Rh2DG658400 Rh2DG664700 Rh2DG665400
rosa_wichuraiana	Rw2G052240 Rw2G052720

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1463
AarI	CACCTGC	1 cut(s)	1876
Acc36I	ACCTGC	2 cut(s)	1876, 3340
Acc65I	GGTACC	1 cut(s)	200
AccB1I	GGYRCC	2 cut(s)	200, 907
AccB7I	CCANNNNNTGG	1 cut(s)	2294
AccBSI	CCGCTC	1 cut(s)	1025
AccI	GTMKAC	3 cut(s)	251, 1380, 1990
AccII	CGCG	4 cut(s)	155, 161, 673, 681
AccIII	TCCGGA	1 cut(s)	3206
AciI	CCGC	4 cut(s)	673, 893, 1023, 2471
AclWI	GGATC	6 cut(s)	565, 749, 1245, 2087, 2357, 3332
AcsI	RAATTY	8 cut(s)	207, 656, 1281, 1835, 1920, 2177, 2663, 3034
AcuI	CTGAAG	3 cut(s)	2483, 2634, 2886
AfaI	GTAC	5 cut(s)	202, 544, 1728, 1826, 3393
AfeI	AGCGCT	1 cut(s)	832
AflII	CTTAAG	2 cut(s)	257, 1265
AgeI	ACCGGT	1 cut(s)	197
AjnI	CCWGG	6 cut(s)	222, 611, 1804, 2121, 2287, 2556
AjuI	GAANNNNNNNTTGG	2 cut(s)	3435, 3467
AleI	CACNNNNGTG	2 cut(s)	1218, 2715
AloI	GAACNNNNNNTCC	2 cut(s)	2829, 2861
Alw21I	GWGCWC	7 cut(s)	585, 1057, 1292, 1628, 2144, 2809, 3015
Alw26I	GTCTC	3 cut(s)	1594, 2339, 2430
Alw44I	GTGCAC	2 cut(s)	1053, 1288
AlwI	GGATC	6 cut(s)	565, 749, 1245, 2087, 2357, 3332
Ama87I	CYCGRG	2 cut(s)	561, 2445
Aor13HI	TCCGGA	1 cut(s)	3206
Aor51HI	AGCGCT	1 cut(s)	832
AoxI	GGCC	4 cut(s)	947, 1092, 1222, 2582
ApaLI	GTGCAC	2 cut(s)	1053, 1288
ApeKI	GCWGC	6 cut(s)	100, 1064, 1750, 1878, 2657, 2969
ApoI	RAATTY	8 cut(s)	207, 656, 1281, 1835, 1920, 2177, 2663, 3034
ArsI	GACNNNNNNTTYG	2 cut(s)	37, 69
AsiGI	ACCGGT	1 cut(s)	197
Asp700I	GAANNNNTTC	2 cut(s)	336, 3447
Asp718I	GGTACC	1 cut(s)	200
AspA2I	CCTAGG	1 cut(s)	2366
AspLEI	GCGC	5 cut(s)	157, 163, 382, 833, 1944
AspS9I	GGNCC	4 cut(s)	53, 886, 2583, 2825
AsuC2I	CCSGG	4 cut(s)	1659, 2446, 2447, 3249
AsuHPI	GGTGA	8 cut(s)	107, 577, 1283, 2024, 2102, 2314, 2485, 3055
AsuNHI	GCTAGC	1 cut(s)	2467
AvaI	CYCGRG	2 cut(s)	561, 2445
AvaII	GGWCC	3 cut(s)	53, 886, 2825
AvrII	CCTAGG	1 cut(s)	2366
BaeGI	GKGCMC	2 cut(s)	1057, 1292
BaeI	ACNNNNGTAYC	2 cut(s)	116, 149
BanI	GGYRCC	2 cut(s)	200, 907
BanII	GRGCYC	2 cut(s)	585, 2784
BauI	CACGAG	1 cut(s)	2808
BbsI	GAAGAC	2 cut(s)	56, 2613
Bbv12I	GWGCWC	7 cut(s)	585, 1057, 1292, 1628, 2144, 2809, 3015
BbvCI	CCTCAGC	2 cut(s)	1914, 2355
BbvI	GCAGC	6 cut(s)	112, 1076, 1762, 1890, 2644, 2956
BceAI	ACGGC	2 cut(s)	1893, 3125
BcgI	CGANNNNNNTGC	2 cut(s)	2304, 2338
BciT130I	CCWGG	6 cut(s)	224, 613, 1806, 2123, 2289, 2558
BciVI	GTATCC	1 cut(s)	1923
BclI	TGATCA	1 cut(s)	3045
BcnI	CCSGG	4 cut(s)	1659, 2446, 2447, 3249
BcoDI	GTCTC	3 cut(s)	1594, 2339, 2430
BfmI	CTRYAG	6 cut(s)	98, 1409, 1566, 1876, 2152, 3333
BfoI	RGCGCY	2 cut(s)	383, 834
BfrI	CTTAAG	2 cut(s)	257, 1265
BfuAI	ACCTGC	2 cut(s)	1876, 3340
BfuI	GTATCC	1 cut(s)	1923
BisI	GCNGC	6 cut(s)	101, 1065, 1751, 1879, 2658, 2970
BlnI	CCTAGG	1 cut(s)	2366
BlpI	GCTNAGC	1 cut(s)	2965
BlsI	GCNGC	6 cut(s)	102, 1066, 1752, 1880, 2659, 2971
BmcAI	AGTACT	1 cut(s)	1826
Bme18I	GGWCC	3 cut(s)	53, 886, 2825
BmeT110I	CYCGRG	2 cut(s)	561, 2445
BmgT120I	GGNCC	4 cut(s)	53, 886, 2583, 2825
BmiI	GGNNCC	6 cut(s)	202, 282, 909, 2209, 2411, 3268
BmrI	ACTGGG	1 cut(s)	2580
BmsI	GCATC	5 cut(s)	652, 1762, 1879, 2156, 2211
BmtI	GCTAGC	1 cut(s)	2471
BmuI	ACTGGG	1 cut(s)	2580
BpiI	GAAGAC	2 cut(s)	56, 2613
BplI	GAGNNNNNCTC	2 cut(s)	3258, 3290
Bpu10I	CCTNAGC	4 cut(s)	1145, 1914, 1937, 2355
Bpu1102I	GCTNAGC	1 cut(s)	2965
BpuEI	CTTGAG	3 cut(s)	421, 887, 1217
BpuMI	CCSGG	4 cut(s)	1659, 2446, 2447, 3249
BsaAI	YACGTR	1 cut(s)	2717
BsaBI	GATNNNNATC	1 cut(s)	1242
BsaWI	WCCGGW	5 cut(s)	197, 587, 2827, 3040, 3206
BsaXI	ACNNNNNCTCC	8 cut(s)	1414, 1444, 2484, 2514, 2829, 2859, 3059, 3089
Bse118I	RCCGGY	2 cut(s)	197, 277
Bse1I	ACTGG	4 cut(s)	391, 2092, 2392, 2586
Bse3DI	GCAATG	2 cut(s)	1951, 3268
Bse8I	GATNNNNATC	1 cut(s)	1242
BseAI	TCCGGA	1 cut(s)	3206
BseBI	CCWGG	6 cut(s)	224, 613, 1806, 2123, 2289, 2558
BseGI	GGATG	8 cut(s)	25, 460, 694, 1520, 1623, 2263, 2947, 2992
BseJI	GATNNNNATC	1 cut(s)	1242
BseMI	GCAATG	2 cut(s)	1951, 3268
BseMII	CTCAG	9 cut(s)	369, 441, 642, 1094, 1905, 1951, 2346, 2979, 3063
BseNI	ACTGG	4 cut(s)	391, 2092, 2392, 2586
BseRI	GAGGAG	3 cut(s)	2220, 3009, 3080
BseSI	GKGCMC	2 cut(s)	1057, 1292
BseXI	GCAGC	6 cut(s)	112, 1076, 1762, 1890, 2644, 2956
Bsh1236I	CGCG	4 cut(s)	155, 161, 673, 681
Bsh1285I	CGRYCG	2 cut(s)	179, 198
BshFI	GGCC	4 cut(s)	949, 1094, 1224, 2584
BshNI	GGYRCC	2 cut(s)	200, 907
BshTI	ACCGGT	1 cut(s)	197
BsiEI	CGRYCG	2 cut(s)	179, 198
BsiHKAI	GWGCWC	7 cut(s)	585, 1057, 1292, 1628, 2144, 2809, 3015
BsiHKCI	CYCGRG	2 cut(s)	561, 2445
BsiSI	CCGG	9 cut(s)	198, 278, 588, 1659, 2446, 2828, 3041, 3207, 3249
BsmAI	GTCTC	3 cut(s)	1594, 2339, 2430
BsmI	GAATGC	3 cut(s)	338, 2446, 3244
BsnI	GGCC	4 cut(s)	949, 1094, 1224, 2584
BsoBI	CYCGRG	2 cut(s)	561, 2445
Bsp1286I	GDGCHC	8 cut(s)	585, 1057, 1292, 1628, 2144, 2784, 2809, 3015
Bsp13I	TCCGGA	1 cut(s)	3206
Bsp1407I	TGTACA	1 cut(s)	1726
Bsp1720I	GCTNAGC	1 cut(s)	2965
Bsp19I	CCATGG	1 cut(s)	264
BspACI	CCGC	4 cut(s)	673, 893, 1023, 2471
BspANI	GGCC	4 cut(s)	949, 1094, 1224, 2584
BspCNI	CTCAG	9 cut(s)	368, 442, 643, 1093, 1906, 1950, 2347, 2978, 3064
BspEI	TCCGGA	1 cut(s)	3206
BspFNI	CGCG	4 cut(s)	155, 161, 673, 681
BspHI	TCATGA	3 cut(s)	2104, 2569, 2641
BspLI	GGNNCC	6 cut(s)	202, 282, 909, 2209, 2411, 3268
BspMAI	CTGCAG	4 cut(s)	102, 1570, 1880, 3337
BspMI	ACCTGC	2 cut(s)	1876, 3340
BspOI	GCTAGC	1 cut(s)	2471
BspPI	GGATC	6 cut(s)	565, 749, 1245, 2087, 2357, 3332
BspQI	GCTCTTC	1 cut(s)	1899
BspT107I	GGYRCC	2 cut(s)	200, 907
BspTI	CTTAAG	2 cut(s)	257, 1265
BsrBI	CCGCTC	1 cut(s)	1025
BsrDI	GCAATG	2 cut(s)	1951, 3268
BsrFI	RCCGGY	2 cut(s)	197, 277
BsrGI	TGTACA	1 cut(s)	1726
BsrI	ACTGG	4 cut(s)	391, 2092, 2392, 2586
BssAI	RCCGGY	2 cut(s)	197, 277
BssNAI	GTATAC	1 cut(s)	252
BssSI	CACGAG	1 cut(s)	2808
BssT1I	CCWWGG	5 cut(s)	264, 916, 2366, 2784, 3398
Bst1107I	GTATAC	1 cut(s)	252
Bst2BI	CACGAG	1 cut(s)	2808
Bst2UI	CCWGG	6 cut(s)	224, 613, 1806, 2123, 2289, 2558
Bst6I	CTCTTC	4 cut(s)	117, 153, 1471, 1899
BstAFI	CTTAAG	2 cut(s)	257, 1265
BstAPI	GCANNNNNTGC	1 cut(s)	573
BstAUI	TGTACA	1 cut(s)	1726
BstBAI	YACGTR	1 cut(s)	2717
BstC8I	GCNNGC	5 cut(s)	159, 279, 804, 2469, 3346
BstDSI	CCRYGG	1 cut(s)	264
BstENI	CCTNNNNNAGG	2 cut(s)	915, 2120
BstF5I	GGATG	8 cut(s)	25, 460, 694, 1520, 1623, 2263, 2947, 2992
BstFNI	CGCG	4 cut(s)	155, 161, 673, 681
BstH2I	RGCGCY	2 cut(s)	383, 834
BstHHI	GCGC	5 cut(s)	157, 163, 382, 833, 1944
BstMAI	GTCTC	3 cut(s)	1594, 2339, 2430
BstMCI	CGRYCG	2 cut(s)	179, 198
BstNI	CCWGG	6 cut(s)	224, 613, 1806, 2123, 2289, 2558
BstNSI	RCATGY	2 cut(s)	297, 3292
BstSFI	CTRYAG	6 cut(s)	98, 1409, 1566, 1876, 2152, 3333
BstSLI	GKGCMC	2 cut(s)	1057, 1292
BstUI	CGCG	4 cut(s)	155, 161, 673, 681
BstV1I	GCAGC	6 cut(s)	112, 1076, 1762, 1890, 2644, 2956
BstV2I	GAAGAC	2 cut(s)	56, 2613
BstX2I	RGATCY	2 cut(s)	557, 3337
BstXI	CCANNNNNNTGG	1 cut(s)	554
BstYI	RGATCY	2 cut(s)	557, 3337
BstZ17I	GTATAC	1 cut(s)	252
BsuI	GTATCC	1 cut(s)	1923
BsuRI	GGCC	4 cut(s)	949, 1094, 1224, 2584
BtgI	CCRYGG	1 cut(s)	264
BtsCI	GGATG	8 cut(s)	25, 460, 694, 1520, 1623, 2263, 2947, 2992
BtsI	GCAGTG	3 cut(s)	1174, 1403, 3403
BtsIMutI	CAGTG	7 cut(s)	1174, 1403, 1974, 2385, 2477, 3348, 3403
BveI	ACCTGC	2 cut(s)	1876, 3340
Cac8I	GCNNGC	5 cut(s)	159, 279, 804, 2469, 3346
CciI	TCATGA	3 cut(s)	2104, 2569, 2641
CfoI	GCGC	5 cut(s)	157, 163, 382, 833, 1944
Cfr10I	RCCGGY	2 cut(s)	197, 277
Cfr13I	GGNCC	4 cut(s)	53, 886, 2583, 2825
Cfr9I	CCCGGG	1 cut(s)	2445
CsiI	ACCWGGT	1 cut(s)	2556
Csp6I	GTAC	5 cut(s)	201, 543, 1727, 1825, 3392
CspAI	ACCGGT	1 cut(s)	197
CspCI	CAANNNNNGTGG	2 cut(s)	3333, 3368
CviQI	GTAC	5 cut(s)	201, 543, 1727, 1825, 3392
Eam1104I	CTCTTC	4 cut(s)	117, 153, 1471, 1899
EarI	CTCTTC	4 cut(s)	117, 153, 1471, 1899
Ecl136II	GAGCTC	1 cut(s)	583
Eco130I	CCWWGG	5 cut(s)	264, 916, 2366, 2784, 3398
Eco147I	AGGCCT	1 cut(s)	949
Eco24I	GRGCYC	2 cut(s)	585, 2784
Eco32I	GATATC	3 cut(s)	39, 994, 3304
Eco47I	GGWCC	3 cut(s)	53, 886, 2825
Eco47III	AGCGCT	1 cut(s)	832
Eco53kI	GAGCTC	1 cut(s)	583
Eco57I	CTGAAG	3 cut(s)	2483, 2634, 2886
Eco88I	CYCGRG	2 cut(s)	561, 2445
EcoICRI	GAGCTC	1 cut(s)	583
EcoNI	CCTNNNNNAGG	2 cut(s)	915, 2120
EcoO109I	RGGNCCY	2 cut(s)	53, 886
EcoRII	CCWGG	6 cut(s)	222, 611, 1804, 2121, 2287, 2556
EcoRV	GATATC	3 cut(s)	39, 994, 3304
EcoT14I	CCWWGG	5 cut(s)	264, 916, 2366, 2784, 3398
EcoT22I	ATGCAT	1 cut(s)	2171
EcoT38I	GRGCYC	2 cut(s)	585, 2784
ErhI	CCWWGG	5 cut(s)	264, 916, 2366, 2784, 3398
FalI	AAGNNNNNCTT	6 cut(s)	1138, 1170, 1814, 1846, 1878, 1910
FauNDI	CATATG	2 cut(s)	1710, 2404
FbaI	TGATCA	1 cut(s)	3045
FblI	GTMKAC	3 cut(s)	251, 1380, 1990
Fnu4HI	GCNGC	6 cut(s)	101, 1065, 1751, 1879, 2658, 2970
FokI	GGATG	8 cut(s)	12, 467, 701, 1527, 1630, 2270, 2934, 2999
FriOI	GRGCYC	2 cut(s)	585, 2784
Fsp4HI	GCNGC	6 cut(s)	101, 1065, 1751, 1879, 2658, 2970
GlaI	GCGC	5 cut(s)	156, 162, 381, 832, 1943
GluI	GCNGC	6 cut(s)	101, 1065, 1751, 1879, 2658, 2970
HaeII	RGCGCY	2 cut(s)	383, 834
HaeIII	GGCC	4 cut(s)	949, 1094, 1224, 2584
HapII	CCGG	9 cut(s)	198, 278, 588, 1659, 2446, 2828, 3041, 3207, 3249
HhaI	GCGC	5 cut(s)	157, 163, 382, 833, 1944
Hin6I	GCGC	5 cut(s)	155, 161, 380, 831, 1942
HinP1I	GCGC	5 cut(s)	155, 161, 380, 831, 1942
HincII	GTYRAC	2 cut(s)	1991, 3414
HindII	GTYRAC	2 cut(s)	1991, 3414
HindIII	AAGCTT	4 cut(s)	1152, 1955, 2594, 2819
HpaII	CCGG	9 cut(s)	198, 278, 588, 1659, 2446, 2828, 3041, 3207, 3249
HphI	GGTGA	8 cut(s)	107, 577, 1283, 2024, 2102, 2314, 2485, 3055
Hpy99I	CGWCG	1 cut(s)	196
HpyAV	CCTTC	5 cut(s)	78, 523, 983, 1421, 2305
HpyCH4IV	ACGT	3 cut(s)	2716, 2803, 2979
HpySE526I	ACGT	3 cut(s)	2716, 2803, 2979
HspAI	GCGC	5 cut(s)	155, 161, 380, 831, 1942
Kpn2I	TCCGGA	1 cut(s)	3206
KpnI	GGTACC	1 cut(s)	204
KroI	GCCGGC	1 cut(s)	277
KroNI	GCCGGC	1 cut(s)	279
Ksp22I	TGATCA	1 cut(s)	3045
LguI	GCTCTTC	1 cut(s)	1899
LmnI	GCTCC	7 cut(s)	286, 580, 764, 1555, 2207, 3218, 3266
Lsp1109I	GCAGC	6 cut(s)	112, 1076, 1762, 1890, 2644, 2956
LweI	GCATC	5 cut(s)	652, 1762, 1879, 2156, 2211
MabI	ACCWGGT	1 cut(s)	2556
MaeII	ACGT	3 cut(s)	2716, 2803, 2979
MaeIII	GTNAC	7 cut(s)	667, 1169, 2012, 2302, 2383, 2473, 2686
MbiI	CCGCTC	1 cut(s)	1025
MfeI	CAATTG	2 cut(s)	1535, 3387
MflI	RGATCY	2 cut(s)	557, 3337
MhlI	GDGCHC	8 cut(s)	585, 1057, 1292, 1628, 2144, 2784, 2809, 3015
MlyI	GAGTC	4 cut(s)	1386, 2432, 2603, 3083
MmeI	TCCRAC	1 cut(s)	3403
Mph1103I	ATGCAT	1 cut(s)	2171
MroI	TCCGGA	1 cut(s)	3206
MroNI	GCCGGC	1 cut(s)	277
MroXI	GAANNNNTTC	2 cut(s)	336, 3447
MslI	CAYNNNNRTG	7 cut(s)	357, 371, 1162, 1218, 1494, 2646, 2715
MspA1I	CMGCKG	1 cut(s)	2969
MspCI	CTTAAG	2 cut(s)	257, 1265
MspI	CCGG	9 cut(s)	198, 278, 588, 1659, 2446, 2828, 3041, 3207, 3249
MunI	CAATTG	2 cut(s)	1535, 3387
Mva1269I	GAATGC	3 cut(s)	338, 2446, 3244
MvaI	CCWGG	6 cut(s)	224, 613, 1806, 2123, 2289, 2558
MvnI	CGCG	4 cut(s)	155, 161, 673, 681
NaeI	GCCGGC	1 cut(s)	279
NciI	CCSGG	4 cut(s)	1659, 2446, 2447, 3249
NcoI	CCATGG	1 cut(s)	264
NdeI	CATATG	2 cut(s)	1710, 2404
NgoMIV	GCCGGC	1 cut(s)	277
NheI	GCTAGC	1 cut(s)	2467
NlaIV	GGNNCC	6 cut(s)	202, 282, 909, 2209, 2411, 3268
NmuCI	GTSAC	6 cut(s)	1169, 2012, 2302, 2383, 2473, 2686
NsiI	ATGCAT	1 cut(s)	2171
NspI	RCATGY	2 cut(s)	297, 3292
OliI	CACNNNNGTG	2 cut(s)	1218, 2715
PagI	TCATGA	3 cut(s)	2104, 2569, 2641
PaqCI	CACCTGC	1 cut(s)	1876
PceI	AGGCCT	1 cut(s)	949
PciSI	GCTCTTC	1 cut(s)	1899
PctI	GAATGC	3 cut(s)	338, 2446, 3244
PdiI	GCCGGC	1 cut(s)	279
PdmI	GAANNNNTTC	2 cut(s)	336, 3447
PfeI	GAWTC	8 cut(s)	245, 441, 599, 1314, 1501, 1529, 1654, 3319
PflMI	CCANNNNNTGG	1 cut(s)	2294
PfoI	TCCNGGA	3 cut(s)	222, 1657, 3247
PinAI	ACCGGT	1 cut(s)	197
PkrI	GCNGC	6 cut(s)	102, 1066, 1752, 1880, 2659, 2971
Ple19I	CGATCG	1 cut(s)	179
PleI	GAGTC	4 cut(s)	1385, 2431, 2603, 3082
PpsI	GAGTC	4 cut(s)	1385, 2431, 2603, 3082
Ppu21I	YACGTR	1 cut(s)	2717
PpuMI	RGGWCCY	2 cut(s)	53, 886
PsiI	TTATAA	1 cut(s)	1463
Psp124BI	GAGCTC	1 cut(s)	585
Psp5II	RGGWCCY	2 cut(s)	53, 886
Psp6I	CCWGG	6 cut(s)	222, 611, 1804, 2121, 2287, 2556
PspGI	CCWGG	6 cut(s)	222, 611, 1804, 2121, 2287, 2556
PspN4I	GGNNCC	6 cut(s)	202, 282, 909, 2209, 2411, 3268
PspPI	GGNCC	4 cut(s)	53, 886, 2583, 2825
PspPPI	RGGWCCY	2 cut(s)	53, 886
PstI	CTGCAG	4 cut(s)	102, 1570, 1880, 3337
PsuI	RGATCY	2 cut(s)	557, 3337
PvuI	CGATCG	1 cut(s)	179
PvuII	CAGCTG	1 cut(s)	2969
RsaI	GTAC	5 cut(s)	202, 544, 1728, 1826, 3393
RsaNI	GTAC	5 cut(s)	201, 543, 1727, 1825, 3392
RseI	CAYNNNNRTG	7 cut(s)	357, 371, 1162, 1218, 1494, 2646, 2715
SacI	GAGCTC	1 cut(s)	585
SalI	GTCGAC	1 cut(s)	1989
SapI	GCTCTTC	1 cut(s)	1899
SatI	GCNGC	6 cut(s)	101, 1065, 1751, 1879, 2658, 2970
Sau96I	GGNCC	4 cut(s)	53, 886, 2583, 2825
ScaI	AGTACT	1 cut(s)	1826
SchI	GAGTC	4 cut(s)	1386, 2432, 2603, 3083
SduI	GDGCHC	8 cut(s)	585, 1057, 1292, 1628, 2144, 2784, 2809, 3015
SexAI	ACCWGGT	1 cut(s)	2556
SfaNI	GCATC	5 cut(s)	652, 1762, 1879, 2156, 2211
SfcI	CTRYAG	6 cut(s)	98, 1409, 1566, 1876, 2152, 3333
SinI	GGWCC	3 cut(s)	53, 886, 2825
SmaI	CCCGGG	1 cut(s)	2447
SmiMI	CAYNNNNRTG	7 cut(s)	357, 371, 1162, 1218, 1494, 2646, 2715
SmlI	CTYRAG	5 cut(s)	257, 436, 902, 1232, 1265
SmoI	CTYRAG	5 cut(s)	257, 436, 902, 1232, 1265
SseBI	AGGCCT	1 cut(s)	949
SsiI	CCGC	4 cut(s)	673, 893, 1023, 2471
SspI	AATATT	3 cut(s)	880, 1007, 3234
SstI	GAGCTC	1 cut(s)	585
StuI	AGGCCT	1 cut(s)	949
StyI	CCWWGG	5 cut(s)	264, 916, 2366, 2784, 3398
TaiI	ACGT	3 cut(s)	2719, 2806, 2982
TaqII	GACCGA	1 cut(s)	1410
TatI	WGTACW	2 cut(s)	1726, 1824
TfiI	GAWTC	8 cut(s)	245, 441, 599, 1314, 1501, 1529, 1654, 3319
TscAI	CASTG	7 cut(s)	1174, 1410, 1981, 2392, 2484, 3355, 3410
TseFI	GTSAC	6 cut(s)	1169, 2012, 2302, 2383, 2473, 2686
TseI	GCWGC	6 cut(s)	100, 1064, 1750, 1878, 2657, 2969
Tsp45I	GTSAC	6 cut(s)	1169, 2012, 2302, 2383, 2473, 2686
TspGWI	ACGGA	2 cut(s)	1275, 1568
TspMI	CCCGGG	1 cut(s)	2445
TspRI	CASTG	7 cut(s)	1174, 1410, 1981, 2392, 2484, 3355, 3410
Van91I	CCANNNNNTGG	1 cut(s)	2294
Vha464I	CTTAAG	2 cut(s)	257, 1265
VneI	GTGCAC	2 cut(s)	1053, 1288
VpaK11BI	GGWCC	3 cut(s)	53, 886, 2825
XagI	CCTNNNNNAGG	2 cut(s)	915, 2120
XapI	RAATTY	8 cut(s)	207, 656, 1281, 1835, 1920, 2177, 2663, 3034
XbaI	TCTAGA	1 cut(s)	2599
XceI	RCATGY	2 cut(s)	297, 3292
XcmI	CCANNNNNNNNNTGG	1 cut(s)	3405
XmaI	CCCGGG	1 cut(s)	2445
XmaJI	CCTAGG	1 cut(s)	2366
XmiI	GTMKAC	3 cut(s)	251, 1380, 1990
XmnI	GAANNNNTTC	2 cut(s)	336, 3447
ZrmI	AGTACT	1 cut(s)	1826
Zsp2I	ATGCAT	1 cut(s)	2171

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.