Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2B

Orthogroup ID

OG0002006

Physical Location & Seq

Genomic Coordinates Reverse (-)

86907620 .. 86916273

Gene Structure 8654 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2BG651500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3687 bp

ATGGAGAAATTCGAGGTTCAGGAAACGGGTTCAAGCATGGATTCGTATACACTTAAGTCCATGGCAGACTTGCCGGCTCCTTTTCGAGCATGTTTTAATTTTAGGTATTTCAACTCTTTGCAGAGTGAATGCTTTCCTGCTTGTTTCCACTCAGATGTGAACATGGTTGTGTCAGCGCCAACTGGAAGTGGTAAAACAGTCATTTTTGAGCTTTGCATTTTGAGGCTTCTTTCAAGATTCATCTCTGAGGATGGGAGGTTTACACCTACAAATGGAACTCTCAAAACAATCTATATTGCCCCATCAAAGGCTTTAGTTCAGGAGAAGGTTCGTGATTGGACCCAGAAGTTTGGATCTCGGGGCATAGTTTGCTTGGAGCTCACCGGAGATAATGAATCTTACAACACCAGGAGTTTACAGGAAGCAGACATTATTTTAACCACTCCTGAGAAATTTGATGCTGTAACGCGGTATCGCGTAAAGGATGGTGGATTGGGCTTTTTCAGCGATATAGCACTTCTACTTATTGATGAAGTTCATCTGTTGAATGATCCTCGTGGAGCAGTTTTGGAAGCAATAGTTAGCAGAATAAAAATGCTTGCTTACAACCCTGAAATGAAGTCAAGCGCTCTGGCTCATGTTCGTTTTCTAGCTGTGTCTGCCACCATTCCAAATATTGAGGACCTTGCGGAGTGGCTCAAGGTGCCTGTCCAAGGGATTAAAAGGTTTGGAGAAGAAATGAGGCCTGTAAAGCTGACTACAAAAGTTTTTGGCTACGCCCCAGCAAAAAATGATTTTCTATTTGAAAAGCGCCTTCAAAACTATATTTTCGATATCCTTATGCAATATTCAAGAGGGAAGTCCGCTCTAATTTTTTGCTCAACTAGAAAAGGTGCACAAGAAGCAGCACAGATACTTTCTCAGACAGTCATGGCCTTTGGTCATTCAAATCCATTCATTAAAAGCAGAGAACAGCAAGAGAGGCTAAGGGAAGCTTCATTGTCATGCAGTGACAAACAAATGCAATCTTATATGATTTATGGTGTTGGTTATCACAATGGTGGCCTTTGCCTCAAGGATCGTAATCTCATTGAAAGTCTTTTTCTTAAGGGTGATGTTCAAATTCTGTGCACAACAAATACTCTTGCTCATGGAATCAACCTACCAGCACATACAGTAGTCATTAAATCAACACAGCACTTCAACAAAGAAAAAGGAGTCTACATGGAAATTGACCGATCTACACTGCTACAGATGTGTGGGAGGGCAGGTCGCCCACCTTTTGATGATACAGGAATGGTTATAATCATGACAAGAAGAGATACGGTTCACTTGTATGAGAATCTCTTGAATGGATGTGAGATGGTGGAATCACAATTGCTTTCATGTTTGACGGAGCATTTAACTGCAGAGATAGTTCAACTGACTGTTTCTGACATTACAAGGGCAATCGAATGGATGAAGTGCTCTTTCTTGTATGTGAGAATGAAGAAGAATTCCGGACACTATGCAGTTAAAAAAGTGATTTCCAATGATCGTATAGAAAAGCATATGCTAGATGTCTGTGTACAGAAAGTTAATGAGTTGTCGCAGCATCAGATGATATGGACTGATGAAGATGGTTTTCTGTTGAAACCACTAGAACCTGGGAGGCTGATGACAAAGTACTACTTGAAATTTGACACAATGAAATACATTATTCAGACACATGCAAACTGCAGCTTGGAAGATGCTCTTCATGTGATATGCCGTGCTGAGGAAATTTCATGGATACAGCTCAGGCGCAATGAGAAAAAGCTTCTAAATGACATAAACACTGATAAAGATGGTCGACTTCGCTTTCACATTCTTGGTGACAAAGGGAAGCGAAAAAAGCGTATTCAAACCAGAGAAGAAAAGATATTTCTTTTGGCTAATGACTGCTTGACTGGTGATCCATCGGTTCATGATTTATCCCTTACCCAGGATATGAACTCCATATGCTCAAATGGCTGTAGAATTGCTAGATGCATGAAAGAATTTTTTATTTACCAAAAGAATTACAAAGGAGCCTTGAACTCGATGCTTTTGGCAAAGTCTATGTATCAGAAACTCTGGGATGACAGTCCATACTTGCTGAAACAATTACCAGGGATTGGAATGGTGACTGCGAAGGCACTACATTTGATGGGAGTTAAATCATTTGAGACACTAGCTGAGGCTGATCCTAGGAGAATTGAGATAGTCACTGGTCGAAAATACCCATATGGGAACCACATAAAAGAGTCTCTACTGTCTCTACCTCCAAAGGTTGAGTTGAAAGTTGAGGAAACTGAATGCCCGGGGCAAGGGAAACTGAAGCTAGCGGTGACACTGACTAGGCTATCACAGACACTCCAATCAACAAAACGACATTATGCTGATATGATTGTAGGTTCAGAAGAAGATAACCTGGTTCTCTTTCATGAGAAAATAAGAGTTGATGAATTCTCGAGCCCCTATAGCGCAACAGTTCTCCTGTCAAATCCCCAACAAGGAAAGCTGACTGTTAAGGCTGATCTTATATTTGAAGAATACATTGGTATTGATCTAAACCAGGAGCTTATCTTAATGAAGAAAAACAATTTAAGTGCAAATAACAAACGAGGAAAAAAAGCACCCTCTCTTCTTCTACCTGAGGAAGTATGTATCATAGAAGATGATATTTATGAAGGCCCAAGTGAAGTGCATCCAAACTCGACGAAATCCAAAAGGGTAGATGGTCCCATGCCCAGTTTCAAGCTTCTAGATGAAGACTCTGAAGAAGATGAGGTTGCCAACAAAGTTCATGAAGATGAGATTGCTGCCAAATTTCAAACAGATGACAAAGTTGTGACACAACAAACAGTATTTGACCACATACGTGAAAAGGCAAAGAACTTCCCTGTCTTGGTGTCATCAAATAGTGTGCTTCCTCCATCATCAGAGCCCTTGGAATTAGCAAGGAAACGTGCTCGTGAGAAGAAGCTTGGACCGGAAAGTGGAGTAGAGGTTCTGGAAGAATTAGAATGGAATAAAGTTCAAAGACGAACTGTGGTCAATCCATCTTCAGAACTTAAAGAAGCAGAACAACATCTTTCTAGTATCAAAAATCATCCAAATCCCAGAAGCTCAGCTGCCCTGAACGTCATGGATGGCAGAGCTGGGCCTCATACTGACCCTGATGTCAAACCTCTAACAGGAGAAACAATATTCGAGCACATACGCACAAAAGCTAAGAATTTTCCGGTGATCAATAAACCAAATGTGGAGGAGGCTGAGTCTTTAATCCAGACAAAACAACGCCTATATAAGACTGAACCAGAGTTTAGCATGGATATTACTGCCGTGCTGAAAGGAACAAAACCAAATGAGGTTATCAGAGATACTGAATGTGATAGGAGGTTGCTCTCCGGAAGCTCCTGCAACCCTCATGGAATATTGAATGCTTCTCCCCGAGGTTTAAGCAATGGAGCCAACCTCTCATCAAGTAACATGCCATCTTTCGATATCTCCATGTTCAAGAATCCGAAGCGACCTGCAGATCCAGCAAGCACTATGGAGAATACTAGGAAGAAACATCAACACTCTCCAATTGTACCACCAAGGCACTGCTCGTTGACAGCAGCTGGAAAATCCAGAGAAGTAGAAACATTTCTTGGGTTTGAGAGTGTCTTTTCCTTTCTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000280 GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005730 GO:0005737 GO:0006139 GO:0006259 GO:0006310 GO:0006725 GO:0006807 GO:0006996 GO:0007049 GO:0007127 GO:0007131 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010501 GO:0016043 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022402 GO:0022414 GO:0031974 GO:0031981 GO:0034641 GO:0035825 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0048285 GO:0051321 GO:0061982 GO:0070013 GO:0070035 GO:0071704 GO:0071840 GO:0090304 GO:0140013 GO:0140098 GO:1901360 GO:1903046

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD HHH_5 Helicase_C PPR Sec63

Protein Analysis

1228

Amino Acids

138.8

Weight (kDa)

8.1

Isoelectric Point (pI)

44.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	39 - 228	1.5e-29	DEAD/DEAH box helicase
ResIII	PF04851	50 - 185	2.4e-08	Type III restriction enzyme, res subunit
Helicase_C	PF00271	346 - 425	2.1e-08	Helicase conserved C-terminal domain
WHD_SNRNP200	PF23445	457 - 536	2.4e-11	Winged Helix-turn-helix domain
Sec63	PF02889	546 - 860	3.9e-47	Sec63 Brl domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Orthologous Genes (Group: OG0002006)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G27730 AT3G27730 AT3G27730 AT3G27730 AT3G27730 AT3G27730 AT3G27730
fragaria_vesca	FvH4_6g51100
malus_domestica	MD09G1029000.v1.1 MD09G1029100.v1.1
prunus_persica	Prupe.3G288900_v2.0.a1
pyrus_communis	pycom111g02300 pycom111g02310
rosa_chinensis	RchiOBHm_Chr2g0089321 RchiOBHm_Chr2g0172661 RchiOBHm_Chr2g0172671
rosa_laevigata	RLG00000022075 RLG00000022122 RLG00000022126
rosa_multiflora	Rmu_sc0002053.1_g000008
rosa_roxburghii	Rroxscaffold_2G00079680 Rroxscaffold_2G00080200 Rroxscaffold_2G00080420
rosa_rugosa	Rorug01G0489000 Rorug01G0489100 Rorug02G0246000 Rorug02G0560500.1
rosa_samantha	Rh2AG637300 Rh2AG637400 Rh2AG637500 Rh2AG637900 Rh2BG650700 Rh2BG650800 Rh2BG650900 Rh2BG651500 Rh2CG045200 Rh2CG045300 Rh2CG610500 Rh2CG617200 Rh2CG617600 Rh2DG658400 Rh2DG664700 Rh2DG665400
rosa_wichuraiana	Rw2G052240 Rw2G052720

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1304
AasI	GACNNNNNNGTC	1 cut(s)	3182
Acc36I	ACCTGC	2 cut(s)	1259, 3544
AccB1I	GGYRCC	1 cut(s)	703
AccB7I	CCANNNNNTGG	1 cut(s)	2135
AccBSI	CCGCTC	1 cut(s)	866
AccI	GTMKAC	3 cut(s)	47, 1221, 1831
AccII	CGCG	2 cut(s)	469, 477
AccIII	TCCGGA	2 cut(s)	1499, 3410
AciI	CCGC	4 cut(s)	469, 689, 864, 2345
AclWI	GGATC	6 cut(s)	361, 545, 1086, 1928, 2198, 3536
AcuI	CTGAAG	3 cut(s)	2357, 2799, 3051
AfaI	GTAC	3 cut(s)	1569, 1667, 3597
AfeI	AGCGCT	1 cut(s)	628
AflII	CTTAAG	2 cut(s)	53, 1106
AjnI	CCWGG	6 cut(s)	407, 1645, 1962, 2128, 2430, 2574
AjuI	GAANNNNNNNTTGG	4 cut(s)	2541, 2573, 3639, 3671
AleI	CACNNNNGTG	2 cut(s)	1059, 2880
AloI	GAACNNNNNNTCC	2 cut(s)	2994, 3026
Alw21I	GWGCWC	6 cut(s)	381, 898, 1133, 1469, 2974, 3219
Alw26I	GTCTC	3 cut(s)	2180, 2271, 2280
Alw44I	GTGCAC	2 cut(s)	894, 1129
AlwI	GGATC	6 cut(s)	361, 545, 1086, 1928, 2198, 3536
AlwNI	CAGNNNCTG	1 cut(s)	3626
Ama87I	CYCGRG	4 cut(s)	357, 2320, 2470, 3453
Aor13HI	TCCGGA	2 cut(s)	1499, 3410
Aor51HI	AGCGCT	1 cut(s)	628
AoxI	GGCC	5 cut(s)	743, 933, 1063, 2692, 3164
ApaLI	GTGCAC	2 cut(s)	894, 1129
ApeKI	GCWGC	6 cut(s)	905, 1591, 1719, 2822, 3134, 3623
Asp700I	GAANNNNTTC	2 cut(s)	132, 3651
AspA2I	CCTAGG	1 cut(s)	2207
AspLEI	GCGC	5 cut(s)	178, 629, 813, 1785, 2486
AspS9I	GGNCC	6 cut(s)	339, 682, 2693, 2741, 2990, 3164
AsuC2I	CCSGG	2 cut(s)	2321, 2322
AsuHPI	GGTGA	7 cut(s)	373, 1124, 1865, 1943, 2155, 2359, 3259
AsuNHI	GCTAGC	1 cut(s)	2341
AvaI	CYCGRG	4 cut(s)	357, 2320, 2470, 3453
AvaII	GGWCC	4 cut(s)	339, 682, 2741, 2990
AvrII	CCTAGG	1 cut(s)	2207
AxyI	CCTNAGG	1 cut(s)	2655
BaeGI	GKGCMC	2 cut(s)	898, 1133
BanI	GGYRCC	1 cut(s)	703
BanII	GRGCYC	3 cut(s)	381, 2477, 2949
BauI	CACGAG	2 cut(s)	555, 2973
BbsI	GAAGAC	1 cut(s)	2778
Bbv12I	GWGCWC	6 cut(s)	381, 898, 1133, 1469, 2974, 3219
BbvCI	CCTCAGC	2 cut(s)	1755, 2196
BbvI	GCAGC	6 cut(s)	917, 1603, 1731, 2809, 3121, 3635
BceAI	ACGGC	2 cut(s)	1734, 3329
BciT130I	CCWGG	6 cut(s)	409, 1647, 1964, 2130, 2432, 2576
BciVI	GTATCC	1 cut(s)	1764
BclI	TGATCA	1 cut(s)	3249
BcnI	CCSGG	2 cut(s)	2321, 2322
BcoDI	GTCTC	3 cut(s)	2180, 2271, 2280
BfmI	CTRYAG	6 cut(s)	1250, 1407, 1717, 1993, 2479, 3537
BfoI	RGCGCY	3 cut(s)	179, 630, 814
BfrI	CTTAAG	2 cut(s)	53, 1106
BfuAI	ACCTGC	2 cut(s)	1259, 3544
BfuI	GTATCC	1 cut(s)	1764
BisI	GCNGC	6 cut(s)	906, 1592, 1720, 2823, 3135, 3624
BlnI	CCTAGG	1 cut(s)	2207
BlpI	GCTNAGC	1 cut(s)	3130
BlsI	GCNGC	6 cut(s)	907, 1593, 1721, 2824, 3136, 3625
BmcAI	AGTACT	1 cut(s)	1667
Bme1390I	CCNGG	8 cut(s)	409, 1647, 1964, 2130, 2321, 2322, 2432, 2576
Bme18I	GGWCC	4 cut(s)	339, 682, 2741, 2990
BmeT110I	CYCGRG	4 cut(s)	357, 2320, 2470, 3453
BmgT120I	GGNCC	6 cut(s)	339, 682, 2693, 2741, 2990, 3164
BmiI	GGNNCC	7 cut(s)	78, 341, 705, 2050, 2252, 2743, 3472
BmrFI	CCNGG	8 cut(s)	409, 1647, 1964, 2130, 2321, 2322, 2432, 2576
BmrI	ACTGGG	1 cut(s)	2745
BmsI	GCATC	6 cut(s)	448, 1603, 1720, 1997, 2052, 2716
BmtI	GCTAGC	1 cut(s)	2345
BmuI	ACTGGG	1 cut(s)	2745
BpiI	GAAGAC	1 cut(s)	2778
BplI	GAGNNNNNCTC	2 cut(s)	3462, 3494
Bpu10I	CCTNAGC	4 cut(s)	986, 1755, 1778, 2196
Bpu1102I	GCTNAGC	1 cut(s)	3130
BpuEI	CTTGAG	2 cut(s)	683, 1058
BpuMI	CCSGG	2 cut(s)	2321, 2322
BsaAI	YACGTR	1 cut(s)	2882
BsaBI	GATNNNNATC	1 cut(s)	1083
BsaWI	WCCGGW	5 cut(s)	383, 1499, 2992, 3244, 3410
BsaXI	ACNNNNNCTCC	8 cut(s)	1255, 1285, 2358, 2388, 2994, 3024, 3263, 3293
Bse118I	RCCGGY	1 cut(s)	73
Bse1I	ACTGG	4 cut(s)	187, 1933, 2233, 2751
Bse21I	CCTNAGG	1 cut(s)	2655
Bse3DI	GCAATG	2 cut(s)	1792, 3472
Bse8I	GATNNNNATC	1 cut(s)	1083
BseAI	TCCGGA	2 cut(s)	1499, 3410
BseBI	CCWGG	6 cut(s)	409, 1647, 1964, 2130, 2432, 2576
BseGI	GGATG	8 cut(s)	256, 490, 1361, 1464, 2104, 2707, 3112, 3157
BseJI	GATNNNNATC	1 cut(s)	1083
BseMI	GCAATG	2 cut(s)	1792, 3472
BseNI	ACTGG	4 cut(s)	187, 1933, 2233, 2751
BseRI	GAGGAG	1 cut(s)	3284
BseSI	GKGCMC	2 cut(s)	898, 1133
BseXI	GCAGC	6 cut(s)	917, 1603, 1731, 2809, 3121, 3635
BseYI	CCCAGC	2 cut(s)	781, 3161
Bsh1236I	CGCG	2 cut(s)	469, 477
BshFI	GGCC	5 cut(s)	745, 935, 1065, 2694, 3166
BshNI	GGYRCC	1 cut(s)	703
BsiHKAI	GWGCWC	6 cut(s)	381, 898, 1133, 1469, 2974, 3219
BsiHKCI	CYCGRG	4 cut(s)	357, 2320, 2470, 3453
BsiSI	CCGG	7 cut(s)	74, 384, 1500, 2321, 2993, 3245, 3411
BslFI	GGGAC	1 cut(s)	2727
BsmAI	GTCTC	3 cut(s)	2180, 2271, 2280
BsmFI	GGGAC	1 cut(s)	2727
BsmI	GAATGC	3 cut(s)	134, 2321, 3448
BsnI	GGCC	5 cut(s)	745, 935, 1065, 2694, 3166
BsoBI	CYCGRG	4 cut(s)	357, 2320, 2470, 3453
Bsp1286I	GDGCHC	8 cut(s)	381, 898, 1133, 1469, 2477, 2949, 2974, 3219
Bsp13I	TCCGGA	2 cut(s)	1499, 3410
Bsp1407I	TGTACA	1 cut(s)	1567
Bsp1720I	GCTNAGC	1 cut(s)	3130
Bsp19I	CCATGG	1 cut(s)	60
BspACI	CCGC	4 cut(s)	469, 689, 864, 2345
BspANI	GGCC	5 cut(s)	745, 935, 1065, 2694, 3166
BspEI	TCCGGA	2 cut(s)	1499, 3410
BspFNI	CGCG	2 cut(s)	469, 477
BspHI	TCATGA	4 cut(s)	1308, 1945, 2443, 2806
BspLI	GGNNCC	7 cut(s)	78, 341, 705, 2050, 2252, 2743, 3472
BspMAI	CTGCAG	3 cut(s)	1411, 1721, 3541
BspMI	ACCTGC	2 cut(s)	1259, 3544
BspOI	GCTAGC	1 cut(s)	2345
BspPI	GGATC	6 cut(s)	361, 545, 1086, 1928, 2198, 3536
BspQI	GCTCTTC	1 cut(s)	1740
BspT107I	GGYRCC	1 cut(s)	703
BspTI	CTTAAG	2 cut(s)	53, 1106
BsrBI	CCGCTC	1 cut(s)	866
BsrDI	GCAATG	2 cut(s)	1792, 3472
BsrFI	RCCGGY	1 cut(s)	73
BsrGI	TGTACA	1 cut(s)	1567
BsrI	ACTGG	4 cut(s)	187, 1933, 2233, 2751
BssAI	RCCGGY	1 cut(s)	73
BssNAI	GTATAC	1 cut(s)	48
BssSI	CACGAG	2 cut(s)	555, 2973
BssT1I	CCWWGG	5 cut(s)	60, 712, 2207, 2949, 3602
Bst1107I	GTATAC	1 cut(s)	48
Bst2BI	CACGAG	2 cut(s)	555, 2973
Bst2UI	CCWGG	6 cut(s)	409, 1647, 1964, 2130, 2432, 2576
Bst6I	CTCTTC	3 cut(s)	1312, 1740, 2649
BstAFI	CTTAAG	2 cut(s)	53, 1106
BstAPI	GCANNNNNTGC	1 cut(s)	369
BstAUI	TGTACA	1 cut(s)	1567
BstBAI	YACGTR	1 cut(s)	2882
BstC8I	GCNNGC	4 cut(s)	75, 600, 2343, 3550
BstDSI	CCRYGG	1 cut(s)	60
BstENI	CCTNNNNNAGG	3 cut(s)	711, 1961, 3195
BstF5I	GGATG	8 cut(s)	256, 490, 1361, 1464, 2104, 2707, 3112, 3157
BstFNI	CGCG	2 cut(s)	469, 477
BstH2I	RGCGCY	3 cut(s)	179, 630, 814
BstHHI	GCGC	5 cut(s)	178, 629, 813, 1785, 2486
BstMAI	GTCTC	3 cut(s)	2180, 2271, 2280
BstNI	CCWGG	6 cut(s)	409, 1647, 1964, 2130, 2432, 2576
BstNSI	RCATGY	3 cut(s)	93, 1712, 3496
BstSCI	CCNGG	8 cut(s)	407, 1645, 1962, 2128, 2319, 2320, 2430, 2574
BstSFI	CTRYAG	6 cut(s)	1250, 1407, 1717, 1993, 2479, 3537
BstSLI	GKGCMC	2 cut(s)	898, 1133
BstUI	CGCG	2 cut(s)	469, 477
BstV1I	GCAGC	6 cut(s)	917, 1603, 1731, 2809, 3121, 3635
BstV2I	GAAGAC	1 cut(s)	2778
BstX2I	RGATCY	2 cut(s)	353, 3541
BstXI	CCANNNNNNTGG	1 cut(s)	350
BstYI	RGATCY	2 cut(s)	353, 3541
BstZ17I	GTATAC	1 cut(s)	48
Bsu36I	CCTNAGG	1 cut(s)	2655
BsuI	GTATCC	1 cut(s)	1764
BsuRI	GGCC	5 cut(s)	745, 935, 1065, 2694, 3166
BtgI	CCRYGG	1 cut(s)	60
BtsCI	GGATG	8 cut(s)	256, 490, 1361, 1464, 2104, 2707, 3112, 3157
BtsI	GCAGTG	3 cut(s)	1015, 1244, 3607
BtsIMutI	CAGTG	6 cut(s)	1015, 1244, 1815, 2226, 2351, 3607
BveI	ACCTGC	2 cut(s)	1259, 3544
Cac8I	GCNNGC	4 cut(s)	75, 600, 2343, 3550
CaiI	CAGNNNCTG	1 cut(s)	3626
CciI	TCATGA	4 cut(s)	1308, 1945, 2443, 2806
CfoI	GCGC	5 cut(s)	178, 629, 813, 1785, 2486
Cfr10I	RCCGGY	1 cut(s)	73
Cfr13I	GGNCC	6 cut(s)	339, 682, 2693, 2741, 2990, 3164
Cfr9I	CCCGGG	1 cut(s)	2320
CsiI	ACCWGGT	1 cut(s)	2430
Csp6I	GTAC	3 cut(s)	1568, 1666, 3596
CviQI	GTAC	3 cut(s)	1568, 1666, 3596
DrdI	GACNNNNNNGTC	1 cut(s)	3182
DseDI	GACNNNNNNGTC	1 cut(s)	3182
Eam1104I	CTCTTC	3 cut(s)	1312, 1740, 2649
EarI	CTCTTC	3 cut(s)	1312, 1740, 2649
Ecl136II	GAGCTC	1 cut(s)	379
Eco130I	CCWWGG	5 cut(s)	60, 712, 2207, 2949, 3602
Eco147I	AGGCCT	1 cut(s)	745
Eco24I	GRGCYC	3 cut(s)	381, 2477, 2949
Eco32I	GATATC	2 cut(s)	835, 3508
Eco47I	GGWCC	4 cut(s)	339, 682, 2741, 2990
Eco47III	AGCGCT	1 cut(s)	628
Eco53kI	GAGCTC	1 cut(s)	379
Eco57I	CTGAAG	3 cut(s)	2357, 2799, 3051
Eco81I	CCTNAGG	1 cut(s)	2655
Eco88I	CYCGRG	4 cut(s)	357, 2320, 2470, 3453
EcoICRI	GAGCTC	1 cut(s)	379
EcoNI	CCTNNNNNAGG	3 cut(s)	711, 1961, 3195
EcoO109I	RGGNCCY	1 cut(s)	682
EcoRI	GAATTC	2 cut(s)	1495, 2465
EcoRII	CCWGG	6 cut(s)	407, 1645, 1962, 2128, 2430, 2574
EcoRV	GATATC	2 cut(s)	835, 3508
EcoT14I	CCWWGG	5 cut(s)	60, 712, 2207, 2949, 3602
EcoT22I	ATGCAT	1 cut(s)	2012
EcoT38I	GRGCYC	3 cut(s)	381, 2477, 2949
ErhI	CCWWGG	5 cut(s)	60, 712, 2207, 2949, 3602
FalI	AAGNNNNNCTT	6 cut(s)	979, 1011, 1655, 1687, 1719, 1751
FaqI	GGGAC	1 cut(s)	2727
FauNDI	CATATG	3 cut(s)	1551, 1979, 2245
FbaI	TGATCA	1 cut(s)	3249
FblI	GTMKAC	3 cut(s)	47, 1221, 1831
Fnu4HI	GCNGC	6 cut(s)	906, 1592, 1720, 2823, 3135, 3624
FokI	GGATG	8 cut(s)	263, 497, 1368, 1471, 2111, 2694, 3099, 3164
FriOI	GRGCYC	3 cut(s)	381, 2477, 2949
Fsp4HI	GCNGC	6 cut(s)	906, 1592, 1720, 2823, 3135, 3624
GlaI	GCGC	5 cut(s)	177, 628, 812, 1784, 2485
GluI	GCNGC	6 cut(s)	906, 1592, 1720, 2823, 3135, 3624
GsaI	CCCAGC	2 cut(s)	785, 3165
HaeII	RGCGCY	3 cut(s)	179, 630, 814
HaeIII	GGCC	5 cut(s)	745, 935, 1065, 2694, 3166
HapII	CCGG	7 cut(s)	74, 384, 1500, 2321, 2993, 3245, 3411
HhaI	GCGC	5 cut(s)	178, 629, 813, 1785, 2486
Hin6I	GCGC	5 cut(s)	176, 627, 811, 1783, 2484
HinP1I	GCGC	5 cut(s)	176, 627, 811, 1783, 2484
HincII	GTYRAC	2 cut(s)	1832, 3618
HindII	GTYRAC	2 cut(s)	1832, 3618
HindIII	AAGCTT	4 cut(s)	993, 1796, 2759, 2984
HpaII	CCGG	7 cut(s)	74, 384, 1500, 2321, 2993, 3245, 3411
HphI	GGTGA	7 cut(s)	373, 1124, 1865, 1943, 2155, 2359, 3259
Hpy99I	CGWCG	1 cut(s)	2722
HpyAV	CCTTC	4 cut(s)	319, 824, 2146, 2684
HpyCH4IV	ACGT	3 cut(s)	2881, 2968, 3144
HpySE526I	ACGT	3 cut(s)	2881, 2968, 3144
HspAI	GCGC	5 cut(s)	176, 627, 811, 1783, 2484
Kpn2I	TCCGGA	2 cut(s)	1499, 3410
KroI	GCCGGC	1 cut(s)	73
KroNI	GCCGGC	1 cut(s)	75
Ksp22I	TGATCA	1 cut(s)	3249
LguI	GCTCTTC	1 cut(s)	1740
LmnI	GCTCC	8 cut(s)	82, 376, 560, 1396, 2048, 2578, 3422, 3470
Lsp1109I	GCAGC	6 cut(s)	917, 1603, 1731, 2809, 3121, 3635
LweI	GCATC	6 cut(s)	448, 1603, 1720, 1997, 2052, 2716
MabI	ACCWGGT	1 cut(s)	2430
MaeII	ACGT	3 cut(s)	2881, 2968, 3144
MaeIII	GTNAC	8 cut(s)	463, 1010, 1853, 2143, 2224, 2347, 2851, 3488
MbiI	CCGCTC	1 cut(s)	866
MfeI	CAATTG	2 cut(s)	1376, 3591
MflI	RGATCY	2 cut(s)	353, 3541
MhlI	GDGCHC	8 cut(s)	381, 898, 1133, 1469, 2477, 2949, 2974, 3219
MlyI	GAGTC	4 cut(s)	1227, 2273, 2768, 3287
Mph1103I	ATGCAT	1 cut(s)	2012
MroI	TCCGGA	2 cut(s)	1499, 3410
MroNI	GCCGGC	1 cut(s)	73
MroXI	GAANNNNTTC	2 cut(s)	132, 3651
MslI	CAYNNNNRTG	7 cut(s)	153, 167, 1003, 1059, 1335, 2811, 2880
MspA1I	CMGCKG	2 cut(s)	3134, 3626
MspCI	CTTAAG	2 cut(s)	53, 1106
MspI	CCGG	7 cut(s)	74, 384, 1500, 2321, 2993, 3245, 3411
MspR9I	CCNGG	8 cut(s)	409, 1647, 1964, 2130, 2321, 2322, 2432, 2576
MunI	CAATTG	2 cut(s)	1376, 3591
Mva1269I	GAATGC	3 cut(s)	134, 2321, 3448
MvaI	CCWGG	6 cut(s)	409, 1647, 1964, 2130, 2432, 2576
MvnI	CGCG	2 cut(s)	469, 477
NaeI	GCCGGC	1 cut(s)	75
NciI	CCSGG	2 cut(s)	2321, 2322
NcoI	CCATGG	1 cut(s)	60
NdeI	CATATG	3 cut(s)	1551, 1979, 2245
NgoMIV	GCCGGC	1 cut(s)	73
NheI	GCTAGC	1 cut(s)	2341
NlaIV	GGNNCC	7 cut(s)	78, 341, 705, 2050, 2252, 2743, 3472
NmuCI	GTSAC	6 cut(s)	1010, 1853, 2143, 2224, 2347, 2851
NsiI	ATGCAT	1 cut(s)	2012
NspI	RCATGY	3 cut(s)	93, 1712, 3496
OliI	CACNNNNGTG	2 cut(s)	1059, 2880
PaeR7I	CTCGAG	1 cut(s)	2470
PagI	TCATGA	4 cut(s)	1308, 1945, 2443, 2806
PceI	AGGCCT	1 cut(s)	745
PciSI	GCTCTTC	1 cut(s)	1740
PctI	GAATGC	3 cut(s)	134, 2321, 3448
PdiI	GCCGGC	1 cut(s)	75
PdmI	GAANNNNTTC	2 cut(s)	132, 3651
PfeI	GAWTC	7 cut(s)	41, 237, 395, 1155, 1342, 1370, 3523
PflMI	CCANNNNNTGG	1 cut(s)	2135
PkrI	GCNGC	6 cut(s)	907, 1593, 1721, 2824, 3136, 3625
PleI	GAGTC	4 cut(s)	1226, 2272, 2768, 3286
PpsI	GAGTC	4 cut(s)	1226, 2272, 2768, 3286
Ppu21I	YACGTR	1 cut(s)	2882
PpuMI	RGGWCCY	1 cut(s)	682
PsiI	TTATAA	1 cut(s)	1304
Psp124BI	GAGCTC	1 cut(s)	381
Psp5II	RGGWCCY	1 cut(s)	682
Psp6I	CCWGG	6 cut(s)	407, 1645, 1962, 2128, 2430, 2574
PspFI	CCCAGC	2 cut(s)	781, 3161
PspGI	CCWGG	6 cut(s)	407, 1645, 1962, 2128, 2430, 2574
PspN4I	GGNNCC	7 cut(s)	78, 341, 705, 2050, 2252, 2743, 3472
PspPI	GGNCC	6 cut(s)	339, 682, 2693, 2741, 2990, 3164
PspPPI	RGGWCCY	1 cut(s)	682
PstI	CTGCAG	3 cut(s)	1411, 1721, 3541
PstNI	CAGNNNCTG	1 cut(s)	3626
PsuI	RGATCY	2 cut(s)	353, 3541
PvuII	CAGCTG	2 cut(s)	3134, 3626
RsaI	GTAC	3 cut(s)	1569, 1667, 3597
RsaNI	GTAC	3 cut(s)	1568, 1666, 3596
RseI	CAYNNNNRTG	7 cut(s)	153, 167, 1003, 1059, 1335, 2811, 2880
SacI	GAGCTC	1 cut(s)	381
SalI	GTCGAC	1 cut(s)	1830
SapI	GCTCTTC	1 cut(s)	1740
SatI	GCNGC	6 cut(s)	906, 1592, 1720, 2823, 3135, 3624
Sau96I	GGNCC	6 cut(s)	339, 682, 2693, 2741, 2990, 3164
ScaI	AGTACT	1 cut(s)	1667
SchI	GAGTC	4 cut(s)	1227, 2273, 2768, 3287
ScrFI	CCNGG	8 cut(s)	409, 1647, 1964, 2130, 2321, 2322, 2432, 2576
SduI	GDGCHC	8 cut(s)	381, 898, 1133, 1469, 2477, 2949, 2974, 3219
SexAI	ACCWGGT	1 cut(s)	2430
SfaNI	GCATC	6 cut(s)	448, 1603, 1720, 1997, 2052, 2716
SfcI	CTRYAG	6 cut(s)	1250, 1407, 1717, 1993, 2479, 3537
Sfr274I	CTCGAG	1 cut(s)	2470
SinI	GGWCC	4 cut(s)	339, 682, 2741, 2990
SlaI	CTCGAG	1 cut(s)	2470
SmaI	CCCGGG	1 cut(s)	2322
SmiMI	CAYNNNNRTG	7 cut(s)	153, 167, 1003, 1059, 1335, 2811, 2880
SmlI	CTYRAG	5 cut(s)	53, 698, 1073, 1106, 2470
SmoI	CTYRAG	5 cut(s)	53, 698, 1073, 1106, 2470
SseBI	AGGCCT	1 cut(s)	745
SsiI	CCGC	4 cut(s)	469, 689, 864, 2345
SspI	AATATT	4 cut(s)	676, 848, 3210, 3438
SstI	GAGCTC	1 cut(s)	381
StuI	AGGCCT	1 cut(s)	745
StyD4I	CCNGG	8 cut(s)	407, 1645, 1962, 2128, 2319, 2320, 2430, 2574
StyI	CCWWGG	5 cut(s)	60, 712, 2207, 2949, 3602
TaiI	ACGT	3 cut(s)	2884, 2971, 3147
TaqII	GACCGA	1 cut(s)	1251
TatI	WGTACW	2 cut(s)	1567, 1665
TfiI	GAWTC	7 cut(s)	41, 237, 395, 1155, 1342, 1370, 3523
TscAI	CASTG	6 cut(s)	1015, 1251, 1822, 2233, 2358, 3614
TseFI	GTSAC	6 cut(s)	1010, 1853, 2143, 2224, 2347, 2851
TseI	GCWGC	6 cut(s)	905, 1591, 1719, 2822, 3134, 3623
Tsp45I	GTSAC	6 cut(s)	1010, 1853, 2143, 2224, 2347, 2851
TspGWI	ACGGA	1 cut(s)	1409
TspMI	CCCGGG	1 cut(s)	2320
TspRI	CASTG	6 cut(s)	1015, 1251, 1822, 2233, 2358, 3614
Van91I	CCANNNNNTGG	1 cut(s)	2135
Vha464I	CTTAAG	2 cut(s)	53, 1106
VneI	GTGCAC	2 cut(s)	894, 1129
VpaK11BI	GGWCC	4 cut(s)	339, 682, 2741, 2990
XagI	CCTNNNNNAGG	3 cut(s)	711, 1961, 3195
XbaI	TCTAGA	1 cut(s)	2764
XceI	RCATGY	3 cut(s)	93, 1712, 3496
XhoI	CTCGAG	1 cut(s)	2470
XmaI	CCCGGG	1 cut(s)	2320
XmaJI	CCTAGG	1 cut(s)	2207
XmiI	GTMKAC	3 cut(s)	47, 1221, 1831
XmnI	GAANNNNTTC	2 cut(s)	132, 3651
ZrmI	AGTACT	1 cut(s)	1667
Zsp2I	ATGCAT	1 cut(s)	2012

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.