Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0002362

Physical Location & Seq

Genomic Coordinates Forward (+)

3539620 .. 3543824

Gene Structure 4205 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1053400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3747 bp

ATGCTCCAACATTTATATTCTCACTCGCCTCACAGACCCTATATCAACCAGGTTCCATCTCTTCACACTTTCTTCTCACTCGTGACTACTCGCCGCCAATTCTCATGTCTCACGGACCCTTCACCGCCGCCATCTCCGCCGACCCCAGATAACAAAACCCACATAGATTTATCTTCCGTTTGTTTCTCTGGAATTGCCCAGTCTGTGTTTTCGAGAAGCTCCCAATTTTTTGACAAGAACAAAGGCAGAGACTTTGCCAATGCTTCTCTGAAAGACCTTCTTTTGGAGATTTATGATGTGGTGCCCGAATACGCTCGTAGAATTAGGCGGGTTTCGGAGCTAAAACCTGAAGATGTGCTCGGATTATTACTGGGTTTTCGGTTTCAGTGTGGGAGAGTTGGGTTTGAAGTTAGAAAGGTCGAGTCTTTGTGGGAGATTTTCAAGTGGGTTAGTGGACAGAGTAAGGGTTTTAAGCATTTTTCGGAGTCGTATGTGGTCATGGCCTCGATGCTTATTCGAGTGGGGTTGCTTAGAGAAGTTGAATTCTTGCTTTCCACAATGGAAAACCAAGAAATTGTATTGAGTAGTAATGAAGTTTTCAGTGATTTGCTTGAAAGGTATGTTAATGCTGGTGAATCAGAAAGGGCCATTTCGATGTATGATCGAATGAGGCGGCATTTAGTCCCTTCATTATCGTGTTATGATGCTTTTCTTGATCATTTAGTTAAAATGAAGAAAACCAAATTGGCTTTTCGAGTTTGTTGGGATATGGTGGAATTGGGTGTGGATTTGAGAGGTTTGAAGGAGGGTACAGTTGAGAAAATCATAGGATTGCTTTGCAGGGATGGAAAGATACAGGAAGCAAGAAATCTTGTGAAGAAGGCTATGGCTTTTGAGCTCAGGCCTAGCAACTCAGTTCTGTATGAAATTACTTGTGGTTATTGTGAGAAGAAGGACTTTGATGATCTGTTGAGTTTCTATGCCGAAATCAAGTGTGCACCTGATGATCTGGCTGGGAATAGGATTATGCATTCTCTATGTAGCAGTATCGGGACAGGAAGGTCGGAACTATATATGCGAGAATTAGAACATTTAGGCTTCAGTCCTGATGAGTTAACCTTTGGGATCATGATTGGCTGGAGTTGTCGTGAACGAAAACTCAAAAATGCCTTCATTTACTTGTCAAATATGTTGGCAAGACAACTAAAGCCCCATAAGTATACCTATAATGCTCTTATCAGTGGGGTCTTTATGGGGGATATGTGGAAGCATGCTGGGGAAATTTTTGACGAGATGGTGGATAGAGGGGTAACACCTAATCTATCGACTTTCAGAATTCTCTTAGCAGGCTATTGTAAAGCTAGACAATTTGATGAAGCGAAAAGGGTAGTTTTTGATATGGCAGGCCACGGATTGGTTCAAAACTCTTCAGTAGAGGATCCACTATCGAAGGCATTTACAATTTTAGGATTCGACCCATTAGCTGTGAGATTGAAGAGAGATAATGATGTGGGGTTTTCTACAACGGAGTTTTATGATAGTCTTGGAAATGGACTTTATTTGGATACAGACTTGGGTGAGTATGAGAAGAGAGTCACCGAGATCCTAGAAGATTGCTTGGTACCCAACTATAATTCACTTACGATGAAAGAATGTGCTCTTGGAAATTTTAAGTGTGCGTTGATGTTGGTTCATGAAATGGTTCAATGGGGGCAAGAATTGTCTTTTTCTACCTTCTCTGCCTTGCTGAAAGGGCTCTCTGCTTCCCCTTCGCATATAAAGGGAATTGCCAATATTGTGGATAAAAAGCTTCACTTGGTAAATCAGCTCGACGAAGAAATTCCAAATTTTATTGTCCAAGCATACATCAAGAAAGGATTGACATCTGATGGGTGGAGAATGTTGAATGAAATGTTCCAAAGGCATCTGAAAATCAATAACGAGACGTATACTGCTGTAATTAAAGGTTTATGCAGGAGAGGAAACTTGAAGGAGCTGCATGTTTGTTGGGATTTTGCTCAACACGACAGATGGTTGCCAGGGTTCAAAGATTGTAAATCTTTAATAGAATGCCTTTGCAAGAAAGAAATGATAACAAAAACGGTGCAGCTTCTTGAAAGTATGCTTATATCCTTCCCTCACTCAAGGTTAGATATATGTCATATGTTCATAGAAAATCTTTCCATTCAAGGTTTTACAAGAACTGCGCATGTATTGTTGGAAGAACTTGAACAGCGTGGTGGCATCCTCGATCGTATGGCTTATAGGTATCTTATAAGGGGATTGTGTAAGGAGAGAGAGTTTCATGTAGCTTTTACGATACTGGACAATATGCTGGCTAGAAATTTGGTGCCATGTTCGGATGTTTTAGTCCTATTAATTCCTCAGTTGTGTAGGGCTGGTAGATATGAAAAAGCCATTTATTTGAAAGAGATTGGTCTAAAAGAGAAATCTTATTCTCCACTTACCATTGATCGTGCATTATTCGAAGGCTGTTGTATTACAGGGAAAGTTGGCGAAGCAACCGCTCTAATACAGAGTATGGTATTGAAGGGGCTACATCCGGATGCTGAAGTTTACAATATTCTGGTTCAGGGTCATTGTAAGATTAACAACTTAAAGAAAGTGAGGGAGTTACTTGGTATCATGATAAGGAACAGTTTCAGCATTTCATTCTCAACTTTCCGCAATTTGGTGCGGTTGATGTGTGTGGAAGGTAGGGTTCTTCATTTGTTGAGTCTGAAGGAGCTCATGATTGGACAAAGTGAGTGTCATGGTCTCACCATTCACAATATTATGATTTTCTATCTTTTCCAAACCGGGAATGCTTTGCTTGTCAATAAAGTGGTAGATCATTTGCAAGAGGAAAAATTGCGGCTCGATGAAGTAACTTACAATTTTCTTGTTTATGGGTTTTCTCGGTGTAAAGATGTGTCAAGTGCTGTGGACCATTTGTGTACTATGATCTCCAAGGATTTCAGGCCAAGCAACCGCAACTTGAGAATGGTTATAACCAGCTTGTGTGGTATTGGGGAGCTTGAGAAAGCCGTGGGGTTGTGTCGAGAAATGGAATTGAGAGGCTGGGTTCATGATTCAATCATTCAAAATGCGATTGTTGAGGGCCTTCTTTCTCAGGGTAAGCTTGAAGAAGCAGAAGGTTTTCTTGACAGGATGGTAGAGAAATGTCTAGTTCCTGAAAATATCAACTATGATAATCTAATTAAACGATTTTGCTCTTATGGAAGACTGAGCAAGGCAGTTGACCTGCTAAACATAGTGTTGAAGAAAGGAAATCTTCCGGCCTCTACCAGTTATGATTCTGTCATCAGCTTTTGCTGCGTGGTTAATAAACTGGACCAAGCTATGGATTTTCTTACAGAGATTTTGGACAGAAATCTCAAGCCAAGCATCAACACTTGGGACATTCTTGTTCACAGTTTATGTCGGGATGGACAAACTGCAGAAGCAGAACGGCTTTTGAATTCTATGGTTTGTGTAGGCGAACCAGTGACCAGGCAGATATATTTGTCTGTAATTAATAGGTATCGTTCAGAAAACAATCTCAGGAAGGCATCAGAGCTCATGCAAAAAATGCAAGAGAGTGGTTTTGAGCCAGACTTTGAGACACACTGGTCTCTCATAAGCAATCTAAGCAATTCTCGTGACAAGGACAACACAAACAGCGGTGGGGGTTTCCTGTCAAGGCTTCTCTCTGCAAGTGGATTTTCCCGGAACAAGAATTCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0048037 GO:0051536 GO:0051540 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2

Protein Analysis

1249

Amino Acids

142.23

Weight (kDa)

7.62

Isoelectric Point (pI)

38.25

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	369 - 416	2.6e-07	PPR repeat family
PPR_2	PF13041	408 - 453	3e-11	PPR repeat family
PPR_1	PF12854	435 - 467	9.7e-06	PPR repeat
PPR_2	PF13041	616 - 659	3.6e-09	PPR repeat family
PPR_2	PF13041	828 - 869	1.9e-06	PPR repeat family
TPR_24	PF23276	940 - 1053	3e-08	Fungal tetratrico peptide repeats
PPR_2	PF13041	1101 - 1149	3.3e-07	PPR repeat family
PPR_1	PF12854	1131 - 1164	3.1e-07	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0002362)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G15280
fragaria_vesca	FvH4_6g47953 FvH4_6g48150 FvH4_6g48150 FvH4_6g48150 FvH4_6g48150 FvH4_6g48150 FvH4_6g48880 FvH4_6g48880
malus_domestica	MD09G1053400.v1.1
prunus_persica	Prupe.3G265200_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0167491 RchiOBHm_Chr2g0167571 RchiOBHm_Chr2g0167581
rosa_laevigata	RLG00000021724 RLG00000021736
rosa_multiflora	Rmu_sc0000056.1_g000010 Rmu_sc0000056.1_g000011 Rmu_sc0001970.1_g000002
rosa_roxburghii	Rroxscaffold_2G00083370 Rroxscaffold_2G00083410 Rroxscaffold_2G00083440
rosa_rugosa	Rorug02G0530600
rosa_samantha	Rh2AG597900 Rh2AG598800 Rh2AG599200 Rh2AG599300 Rh2AG599400 Rh2BG609400 Rh2BG609800 Rh2BG609900 Rh2BG610000 Rh2CG580300 Rh2DG620700 Rh2DG621000 Rh2DG621100 Rh2DG621900 Rh2DG622000 Rh2DG622100 Rh2DG622600 Rh2DG622700
rosa_wichuraiana	Rw2G049750

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	2276, 3011
AasI	GACNNNNNNGTC	2 cut(s)	1060, 3631
Acc16I	TGCGCA	1 cut(s)	2208
Acc36I	ACCTGC	1 cut(s)	3273
Acc65I	GGTACC	1 cut(s)	1621
AccB1I	GGYRCC	3 cut(s)	301, 1621, 2350
AccB7I	CCANNNNNTGG	2 cut(s)	1414, 3364
AccBSI	CCGCTC	1 cut(s)	2528
AccI	GTMKAC	2 cut(s)	1220, 1949
AccIII	TCCGGA	1 cut(s)	2563
AclWI	GGATC	4 cut(s)	1133, 1433, 1446, 1597
AcsI	RAATTY	9 cut(s)	542, 1281, 1335, 1666, 1839, 1846, 2344, 3481, 3739
AcuI	CTGAAG	5 cut(s)	369, 1084, 1413, 2592, 2762
AfaI	GTAC	3 cut(s)	811, 1623, 2959
AfiI	CCNNNNNNNGG	4 cut(s)	283, 1414, 2692, 3364
AjnI	CCWGG	3 cut(s)	48, 2038, 3512
AjuI	GAANNNNNNNTTGG	4 cut(s)	728, 760, 1644, 1676
Alw21I	GWGCWC	6 cut(s)	360, 900, 1000, 1660, 2751, 3582
Alw26I	GTCTC	6 cut(s)	113, 243, 1937, 2783, 3617, 3639
Alw44I	GTGCAC	1 cut(s)	996
AlwI	GGATC	4 cut(s)	1133, 1433, 1446, 1597
Aor13HI	TCCGGA	1 cut(s)	2563
AoxI	GGCC	7 cut(s)	501, 645, 902, 1405, 2981, 3121, 3300
ApaLI	GTGCAC	1 cut(s)	996
ApeKI	GCWGC	3 cut(s)	1996, 2107, 3336
ApoI	RAATTY	9 cut(s)	542, 1281, 1335, 1666, 1839, 1846, 2344, 3481, 3739
ArsI	GACNNNNNNTTYG	2 cut(s)	2755, 2787
AseI	ATTAAT	2 cut(s)	2378, 3537
Asp700I	GAANNNNTTC	4 cut(s)	1701, 1839, 2660, 3159
Asp718I	GGTACC	1 cut(s)	1621
AspLEI	GCGC	1 cut(s)	2209
AspS9I	GGNCC	5 cut(s)	115, 645, 2947, 3121, 3355
AsuC2I	CCSGG	2 cut(s)	2821, 3730
AsuHPI	GGTGA	5 cut(s)	114, 644, 1588, 1589, 2773
AsuII	TTCGAA	1 cut(s)	2487
AvaII	GGWCC	3 cut(s)	115, 2947, 3355
BaeGI	GKGCMC	2 cut(s)	306, 1000
BamHI	GGATCC	1 cut(s)	1438
BanI	GGYRCC	3 cut(s)	301, 1621, 2350
BanII	GRGCYC	4 cut(s)	900, 1758, 2751, 3582
BauI	CACGAG	2 cut(s)	80, 3660
BbsI	GAAGAC	1 cut(s)	3250
Bbv12I	GWGCWC	6 cut(s)	360, 900, 1000, 1660, 2751, 3582
BbvI	GCAGC	3 cut(s)	1983, 2119, 3323
BccI	CCATC	8 cut(s)	64, 139, 839, 1288, 1883, 2025, 3166, 3443
BceAI	ACGGC	2 cut(s)	3032, 3488
BciT130I	CCWGG	3 cut(s)	50, 2040, 3514
BciVI	GTATCC	1 cut(s)	1558
BclI	TGATCA	1 cut(s)	715
BcnI	CCSGG	2 cut(s)	2821, 3730
BcoDI	GTCTC	6 cut(s)	113, 243, 1937, 2783, 3617, 3639
BfaI	CTAG	5 cut(s)	906, 1362, 1607, 2340, 3188
BfmI	CTRYAG	1 cut(s)	3459
BfuAI	ACCTGC	1 cut(s)	3273
BfuI	GTATCC	1 cut(s)	1558
BisI	GCNGC	7 cut(s)	94, 128, 674, 1997, 2108, 2876, 3337
BlsI	GCNGC	7 cut(s)	95, 129, 675, 1998, 2109, 2877, 3338
Bme1390I	CCNGG	5 cut(s)	50, 2040, 2821, 3514, 3730
Bme18I	GGWCC	3 cut(s)	115, 2947, 3355
BmgT120I	GGNCC	5 cut(s)	115, 645, 2947, 3121, 3355
BmiI	GGNNCC	6 cut(s)	54, 117, 303, 1440, 1623, 2352
BmrFI	CCNGG	5 cut(s)	50, 2040, 2821, 3514, 3730
BmrI	ACTGGG	2 cut(s)	193, 380
BmsI	GCATC	7 cut(s)	498, 694, 1933, 2253, 2557, 3417, 3581
BmuI	ACTGGG	2 cut(s)	193, 380
BpiI	GAAGAC	1 cut(s)	3250
BpmI	CTGGAG	1 cut(s)	1159
Bpu10I	CCTNAGC	1 cut(s)	899
Bpu14I	TTCGAA	1 cut(s)	2487
BpuEI	CTTGAG	4 cut(s)	2128, 3019, 3059, 3383
BpuMI	CCSGG	2 cut(s)	2821, 3730
BsaBI	GATNNNNATC	1 cut(s)	1882
BsaI	GGTCTC	2 cut(s)	2783, 3639
BsaJI	CCNNGG	4 cut(s)	1408, 2039, 2970, 3048
BsaWI	WCCGGW	1 cut(s)	2563
BsaXI	ACNNNNNCTCC	4 cut(s)	1132, 1162, 2285, 2315
Bsc4I	CCNNNNNNNGG	4 cut(s)	283, 1414, 2692, 3364
Bse1I	ACTGG	7 cut(s)	199, 375, 2328, 3309, 3357, 3506, 3635
Bse8I	GATNNNNATC	1 cut(s)	1882
BseAI	TCCGGA	1 cut(s)	2563
BseBI	CCWGG	3 cut(s)	50, 2040, 3514
BseDI	CCNNGG	4 cut(s)	1408, 2039, 2970, 3048
BseGI	GGATG	7 cut(s)	850, 2244, 2368, 2560, 2572, 3177, 3454
BseJI	GATNNNNATC	1 cut(s)	1882
BseLI	CCNNNNNNNGG	4 cut(s)	283, 1414, 2692, 3364
BseMII	CTCAG	6 cut(s)	913, 927, 2399, 3146, 3239, 3577
BseNI	ACTGG	7 cut(s)	199, 375, 2328, 3309, 3357, 3506, 3635
BseSI	GKGCMC	2 cut(s)	306, 1000
BseXI	GCAGC	3 cut(s)	1983, 2119, 3323
BseYI	CCCAGC	3 cut(s)	1013, 1274, 3081
BsgI	GTGCAG	1 cut(s)	2126
Bsh1285I	CGRYCG	1 cut(s)	2254
BshFI	GGCC	7 cut(s)	503, 647, 904, 1407, 2983, 3123, 3302
BshNI	GGYRCC	3 cut(s)	301, 1621, 2350
BsiEI	CGRYCG	1 cut(s)	2254
BsiHKAI	GWGCWC	6 cut(s)	360, 900, 1000, 1660, 2751, 3582
BsiSI	CCGG	4 cut(s)	2564, 2820, 3299, 3730
BslFI	GGGAC	3 cut(s)	668, 1066, 3434
BslI	CCNNNNNNNGG	4 cut(s)	283, 1414, 2692, 3364
BsmAI	GTCTC	6 cut(s)	113, 243, 1937, 2783, 3617, 3639
BsmBI	CGTCTC	1 cut(s)	1937
BsmFI	GGGAC	3 cut(s)	668, 1066, 3434
BsmI	GAATGC	3 cut(s)	1030, 2075, 2830
BsnI	GGCC	7 cut(s)	503, 647, 904, 1407, 2983, 3123, 3302
Bso31I	GGTCTC	2 cut(s)	2783, 3639
Bsp119I	TTCGAA	1 cut(s)	2487
Bsp1286I	GDGCHC	8 cut(s)	306, 360, 900, 1000, 1660, 1758, 2751, 3582
Bsp13I	TCCGGA	1 cut(s)	2563
BspANI	GGCC	7 cut(s)	503, 647, 904, 1407, 2983, 3123, 3302
BspCNI	CTCAG	6 cut(s)	912, 926, 2398, 3145, 3240, 3576
BspEI	TCCGGA	1 cut(s)	2563
BspHI	TCATGA	5 cut(s)	1128, 1693, 2646, 2751, 3088
BspLI	GGNNCC	6 cut(s)	54, 117, 303, 1440, 1623, 2352
BspMAI	CTGCAG	1 cut(s)	3463
BspMI	ACCTGC	1 cut(s)	3273
BspPI	GGATC	4 cut(s)	1133, 1433, 1446, 1597
BspT104I	TTCGAA	1 cut(s)	2487
BspT107I	GGYRCC	3 cut(s)	301, 1621, 2350
BspTNI	GGTCTC	2 cut(s)	2783, 3639
BsrBI	CCGCTC	1 cut(s)	2528
BsrI	ACTGG	7 cut(s)	199, 375, 2328, 3309, 3357, 3506, 3635
BssECI	CCNNGG	4 cut(s)	1408, 2039, 2970, 3048
BssNAI	GTATAC	2 cut(s)	1221, 1950
BssSI	CACGAG	2 cut(s)	80, 3660
BssT1I	CCWWGG	1 cut(s)	2970
Bst1107I	GTATAC	2 cut(s)	1221, 1950
Bst2BI	CACGAG	2 cut(s)	80, 3660
Bst2UI	CCWGG	3 cut(s)	50, 2040, 3514
Bst4CI	ACNGT	4 cut(s)	814, 2104, 2660, 3437
Bst6I	CTCTTC	4 cut(s)	66, 1432, 1490, 1583
BstAPI	GCANNNNNTGC	1 cut(s)	3592
BstBI	TTCGAA	1 cut(s)	2487
BstC8I	GCNNGC	4 cut(s)	1272, 1348, 1405, 2337
BstDEI	CTNAG	9 cut(s)	530, 899, 913, 1342, 2385, 3132, 3248, 3563, 3650
BstDSI	CCRYGG	2 cut(s)	1408, 3048
BstF5I	GGATG	7 cut(s)	850, 2244, 2368, 2560, 2572, 3177, 3454
BstHHI	GCGC	1 cut(s)	2209
BstMAI	GTCTC	6 cut(s)	113, 243, 1937, 2783, 3617, 3639
BstMCI	CGRYCG	1 cut(s)	2254
BstMWI	GCNNNNNNNGC	5 cut(s)	136, 1753, 3336, 3592, 3651
BstNI	CCWGG	3 cut(s)	50, 2040, 3514
BstNSI	RCATGY	3 cut(s)	1274, 2003, 2213
BstSCI	CCNGG	5 cut(s)	48, 2038, 2819, 3512, 3728
BstSFI	CTRYAG	1 cut(s)	3459
BstSLI	GKGCMC	2 cut(s)	306, 1000
BstV1I	GCAGC	3 cut(s)	1983, 2119, 3323
BstV2I	GAAGAC	1 cut(s)	3250
BstX2I	RGATCY	2 cut(s)	1438, 1602
BstXI	CCANNNNNNTGG	1 cut(s)	1798
BstYI	RGATCY	2 cut(s)	1438, 1602
BstZ17I	GTATAC	2 cut(s)	1221, 1950
BsuI	GTATCC	1 cut(s)	1558
BsuRI	GGCC	7 cut(s)	503, 647, 904, 1407, 2983, 3123, 3302
BtgI	CCRYGG	2 cut(s)	1408, 3048
BtsCI	GGATG	7 cut(s)	850, 2244, 2368, 2560, 2572, 3177, 3454
BtsIMutI	CAGTG	5 cut(s)	392, 607, 1246, 3513, 3628
BveI	ACCTGC	1 cut(s)	3273
Cac8I	GCNNGC	4 cut(s)	1272, 1348, 1405, 2337
CciI	TCATGA	5 cut(s)	1128, 1693, 2646, 2751, 3088
CfoI	GCGC	1 cut(s)	2209
Cfr13I	GGNCC	5 cut(s)	115, 645, 2947, 3121, 3355
CsiI	ACCWGGT	1 cut(s)	48
Csp6I	GTAC	3 cut(s)	810, 1622, 2958
CspCI	CAANNNNNGTGG	2 cut(s)	2925, 2960
CviQI	GTAC	3 cut(s)	810, 1622, 2958
DdeI	CTNAG	9 cut(s)	530, 899, 913, 1342, 2385, 3132, 3248, 3563, 3650
DrdI	GACNNNNNNGTC	2 cut(s)	1060, 3631
DseDI	GACNNNNNNGTC	2 cut(s)	1060, 3631
Eam1104I	CTCTTC	4 cut(s)	66, 1432, 1490, 1583
EarI	CTCTTC	4 cut(s)	66, 1432, 1490, 1583
EciI	GGCGGA	1 cut(s)	126
Ecl136II	GAGCTC	3 cut(s)	898, 2749, 3580
Eco130I	CCWWGG	1 cut(s)	2970
Eco147I	AGGCCT	1 cut(s)	904
Eco24I	GRGCYC	4 cut(s)	900, 1758, 2751, 3582
Eco31I	GGTCTC	2 cut(s)	2783, 3639
Eco47I	GGWCC	3 cut(s)	115, 2947, 3355
Eco53kI	GAGCTC	3 cut(s)	898, 2749, 3580
Eco57I	CTGAAG	5 cut(s)	369, 1084, 1413, 2592, 2762
EcoICRI	GAGCTC	3 cut(s)	898, 2749, 3580
EcoO109I	RGGNCCY	1 cut(s)	3121
EcoRI	GAATTC	4 cut(s)	542, 1335, 3481, 3739
EcoRII	CCWGG	3 cut(s)	48, 2038, 3512
EcoT14I	CCWWGG	1 cut(s)	2970
EcoT22I	ATGCAT	1 cut(s)	1032
EcoT38I	GRGCYC	4 cut(s)	900, 1758, 2751, 3582
ErhI	CCWWGG	1 cut(s)	2970
Esp3I	CGTCTC	1 cut(s)	1937
FaqI	GGGAC	3 cut(s)	668, 1066, 3434
FauI	CCCGC	1 cut(s)	321
FauNDI	CATATG	1 cut(s)	2163
FbaI	TGATCA	1 cut(s)	715
FblI	GTMKAC	2 cut(s)	1220, 1949
Fnu4HI	GCNGC	7 cut(s)	94, 128, 674, 1997, 2108, 2876, 3337
FokI	GGATG	7 cut(s)	857, 2231, 2375, 2547, 2579, 3184, 3461
FriOI	GRGCYC	4 cut(s)	900, 1758, 2751, 3582
Fsp4HI	GCNGC	7 cut(s)	94, 128, 674, 1997, 2108, 2876, 3337
FspBI	CTAG	5 cut(s)	906, 1362, 1607, 2340, 3188
FspI	TGCGCA	1 cut(s)	2208
GlaI	GCGC	1 cut(s)	2208
GluI	GCNGC	7 cut(s)	94, 128, 674, 1997, 2108, 2876, 3337
GsaI	CCCAGC	3 cut(s)	1017, 1278, 3085
GsuI	CTGGAG	1 cut(s)	1159
HaeIII	GGCC	7 cut(s)	503, 647, 904, 1407, 2983, 3123, 3302
HapII	CCGG	4 cut(s)	2564, 2820, 3299, 3730
HhaI	GCGC	1 cut(s)	2209
Hin6I	GCGC	1 cut(s)	2207
HinP1I	GCGC	1 cut(s)	2207
HincII	GTYRAC	2 cut(s)	1116, 3262
HindII	GTYRAC	2 cut(s)	1116, 3262
HindIII	AAGCTT	2 cut(s)	1808, 3140
HinfI	GANTC	8 cut(s)	422, 485, 635, 1470, 1593, 2737, 3092, 3317
HpaI	GTTAAC	1 cut(s)	1116
HpaII	CCGG	4 cut(s)	2564, 2820, 3299, 3730
HphI	GGTGA	5 cut(s)	114, 644, 1588, 1589, 2773
Hpy99I	CGWCG	1 cut(s)	1835
HpyCH4III	ACNGT	4 cut(s)	814, 2104, 2660, 3437
HpyCH4IV	ACGT	1 cut(s)	1946
HpyF10VI	GCNNNNNNNGC	5 cut(s)	136, 1753, 3336, 3592, 3651
HpyF3I	CTNAG	9 cut(s)	530, 899, 913, 1342, 2385, 3132, 3248, 3563, 3650
HpySE526I	ACGT	1 cut(s)	1946
HspAI	GCGC	1 cut(s)	2207
Kpn2I	TCCGGA	1 cut(s)	2563
KpnI	GGTACC	1 cut(s)	1625
Ksp22I	TGATCA	1 cut(s)	715
KspAI	GTTAAC	1 cut(s)	1116
LmnI	GCTCC	6 cut(s)	9, 224, 337, 1993, 2746, 3034
Lsp1109I	GCAGC	3 cut(s)	1983, 2119, 3323
LweI	GCATC	7 cut(s)	498, 694, 1933, 2253, 2557, 3417, 3581
MabI	ACCWGGT	1 cut(s)	48
MaeI	CTAG	5 cut(s)	906, 1362, 1607, 2340, 3188
MaeII	ACGT	1 cut(s)	1946
MaeIII	GTNAC	7 cut(s)	82, 1309, 1594, 2634, 2887, 3508, 3662
MbiI	CCGCTC	1 cut(s)	2528
MflI	RGATCY	2 cut(s)	1438, 1602
MhlI	GDGCHC	8 cut(s)	306, 360, 900, 1000, 1660, 1758, 2751, 3582
MlyI	GAGTC	4 cut(s)	431, 494, 1602, 2746
MmeI	TCCRAC	3 cut(s)	31, 1044, 2199
Mph1103I	ATGCAT	1 cut(s)	1032
MroI	TCCGGA	1 cut(s)	2563
MroXI	GAANNNNTTC	4 cut(s)	1701, 1839, 2660, 3159
MslI	CAYNNNNRTG	3 cut(s)	653, 694, 2565
MspA1I	CMGCKG	1 cut(s)	3684
MspI	CCGG	4 cut(s)	2564, 2820, 3299, 3730
MspR9I	CCNGG	5 cut(s)	50, 2040, 2821, 3514, 3730
Mva1269I	GAATGC	3 cut(s)	1030, 2075, 2830
MvaI	CCWGG	3 cut(s)	50, 2040, 3514
MwoI	GCNNNNNNNGC	5 cut(s)	136, 1753, 3336, 3592, 3651
NciI	CCSGG	2 cut(s)	2821, 3730
NdeI	CATATG	1 cut(s)	2163
NlaIV	GGNNCC	6 cut(s)	54, 117, 303, 1440, 1623, 2352
NmuCI	GTSAC	4 cut(s)	82, 1594, 3508, 3662
NsbI	TGCGCA	1 cut(s)	2208
NsiI	ATGCAT	1 cut(s)	1032
NspI	RCATGY	3 cut(s)	1274, 2003, 2213
NspV	TTCGAA	1 cut(s)	2487
PaeI	GCATGC	1 cut(s)	1274
PagI	TCATGA	5 cut(s)	1128, 1693, 2646, 2751, 3088
PceI	AGGCCT	1 cut(s)	904
PctI	GAATGC	3 cut(s)	1030, 2075, 2830
PdmI	GAANNNNTTC	4 cut(s)	1701, 1839, 2660, 3159
PfeI	GAWTC	4 cut(s)	635, 1470, 3092, 3317
PflMI	CCANNNNNTGG	2 cut(s)	1414, 3364
PfoI	TCCNGGA	1 cut(s)	3728
PkrI	GCNGC	7 cut(s)	95, 129, 675, 1998, 2109, 2877, 3338
Ple19I	CGATCG	1 cut(s)	2254
PleI	GAGTC	4 cut(s)	430, 493, 1601, 2745
PpsI	GAGTC	4 cut(s)	430, 493, 1601, 2745
PshBI	ATTAAT	2 cut(s)	2378, 3537
PsiI	TTATAA	2 cut(s)	2276, 3011
Psp124BI	GAGCTC	3 cut(s)	900, 2751, 3582
Psp6I	CCWGG	3 cut(s)	48, 2038, 3512
PspFI	CCCAGC	3 cut(s)	1013, 1274, 3081
PspGI	CCWGG	3 cut(s)	48, 2038, 3512
PspN4I	GGNNCC	6 cut(s)	54, 117, 303, 1440, 1623, 2352
PspPI	GGNCC	5 cut(s)	115, 645, 2947, 3121, 3355
PstI	CTGCAG	1 cut(s)	3463
PsuI	RGATCY	2 cut(s)	1438, 1602
PvuI	CGATCG	1 cut(s)	2254
RsaI	GTAC	3 cut(s)	811, 1623, 2959
RsaNI	GTAC	3 cut(s)	810, 1622, 2958
RseI	CAYNNNNRTG	3 cut(s)	653, 694, 2565
SacI	GAGCTC	3 cut(s)	900, 2751, 3582
SatI	GCNGC	7 cut(s)	94, 128, 674, 1997, 2108, 2876, 3337
Sau96I	GGNCC	5 cut(s)	115, 645, 2947, 3121, 3355
SchI	GAGTC	4 cut(s)	431, 494, 1602, 2746
ScrFI	CCNGG	5 cut(s)	50, 2040, 2821, 3514, 3730
SduI	GDGCHC	8 cut(s)	306, 360, 900, 1000, 1660, 1758, 2751, 3582
SexAI	ACCWGGT	1 cut(s)	48
SfaNI	GCATC	7 cut(s)	498, 694, 1933, 2253, 2557, 3417, 3581
SfcI	CTRYAG	1 cut(s)	3459
SfuI	TTCGAA	1 cut(s)	2487
SinI	GGWCC	3 cut(s)	115, 2947, 3355
SmiMI	CAYNNNNRTG	3 cut(s)	653, 694, 2565
SmlI	CTYRAG	4 cut(s)	2143, 2998, 3038, 3398
SmoI	CTYRAG	4 cut(s)	2143, 2998, 3038, 3398
SphI	GCATGC	1 cut(s)	1274
SseBI	AGGCCT	1 cut(s)	904
SspI	AATATT	3 cut(s)	1795, 2584, 2794
SspMI	CTAG	5 cut(s)	906, 1362, 1607, 2340, 3188
SstI	GAGCTC	3 cut(s)	900, 2751, 3582
StuI	AGGCCT	1 cut(s)	904
StyD4I	CCNGG	5 cut(s)	48, 2038, 2819, 3512, 3728
StyI	CCWWGG	1 cut(s)	2970
TaaI	ACNGT	4 cut(s)	814, 2104, 2660, 3437
TaiI	ACGT	1 cut(s)	1949
TatI	WGTACW	1 cut(s)	2957
TauI	GCSGC	4 cut(s)	96, 130, 676, 2878
TfiI	GAWTC	4 cut(s)	635, 1470, 3092, 3317
TscAI	CASTG	5 cut(s)	392, 607, 1246, 3513, 3635
TseFI	GTSAC	4 cut(s)	82, 1594, 3508, 3662
TseI	GCWGC	3 cut(s)	1996, 2107, 3336
Tsp45I	GTSAC	4 cut(s)	82, 1594, 3508, 3662
TspGWI	ACGGA	4 cut(s)	128, 166, 1425, 1541
TspRI	CASTG	5 cut(s)	392, 607, 1246, 3513, 3635
Van91I	CCANNNNNTGG	2 cut(s)	1414, 3364
VneI	GTGCAC	1 cut(s)	996
VpaK11BI	GGWCC	3 cut(s)	115, 2947, 3355
VspI	ATTAAT	2 cut(s)	2378, 3537
XapI	RAATTY	9 cut(s)	542, 1281, 1335, 1666, 1839, 1846, 2344, 3481, 3739
XceI	RCATGY	3 cut(s)	1274, 2003, 2213
XmiI	GTMKAC	2 cut(s)	1220, 1949
XmnI	GAANNNNTTC	4 cut(s)	1701, 1839, 2660, 3159
XspI	CTAG	5 cut(s)	906, 1362, 1607, 2340, 3188
Zsp2I	ATGCAT	1 cut(s)	1032

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.