Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0002362

Physical Location & Seq

Genomic Coordinates Reverse (-)

77531698 .. 77535495

Gene Structure 3798 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000021724

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3798 bp

ATGAGACGAGACAGCACCACCATAGCTCTATGCAATGAAATGCTTCAAGTTTTGTACACCTGCTCATCGCACAAGCCTCACATCAAACAGGTTGGTTCTCTTTACTCTCTGTTCTCACTCTTCACCACTAACCACCACTTCTCAACTACCACTTCTTCATCCCCGTCATCTTCACCAACACAAAATACCATCACCCAAATAGATTCATCTCCTAATTGTTTCAATGGTATAGCCCAATCTGTCATTTTGAGGTGTTCCCAATATTTTGACAAGAGTAAAGGCAAAGACTTTGCCAATGCTTCTCTAAAAGACCTTCTTTTGGAGATTTCTGATTTAGTGCCTGAACAAACTCGTAGGCTTAGGCGGGTTTCGGAGCCGAAACCCGAAGATGTGCTTGAATTATTACGGGGTTTTGAATTGCAGTGTGCGCAAGTTGGATTTGAAGCTAGAAAGGTTGAGTCTTCGTGGGGAATTTTCAAGTGGGTTAGCGAAAAAGTTGAGGGTTTTAAGCATAAACCTCAGTCATGTGAGGTCATGGCCTCGATGCTTGTCCGAGTGGGATTGCTTAGAGAAGTTGAAGTCTTGCTTTCCACAATGGGAAGTCAAGGAGTTTTGTTGGATAGTCGAGAAATTTACAGTGATTTGATTAAAGGGTATGTTGGTGTTGGTGAGTTAGATAGGGCCATTTCTGTCTATGATCGAATTAGGGGGCGTGTAGTGCCATCATTGCAGTGTTGTTGTGTTCTACTTAATCAATTGGTGGGAATGAGGAAGACCCAATTGGCGTTTCAAGTTTGTAGTGATATGGCAGAAATGGGTTTTGATTTGAGAGATGTGAAGAAGGCCACATTTGAGGGTGTCATTAGACTGCTTTGCAGGGATGGAAAGATTCAGGAGGCAAGAAATTTTGTAAAGAAGGCTGTGGCTTTCGAACTCAAGCCTAGCAACTTAGTTTTAAATGAAGTTGCTTATGGATATTGTGAGAAGAAGGACTTCGATGATTTGATGAGCTTCTATGCTGAGATTAAGTGTGCCCCAGAAGTTATGGCTGGGAATAGGATTATGCATTCTCTTTGTAACCATTTTGGGACAAGAAGAGCAGAACCTTACTTGCAAGAATTGGAAATTTTAGGCTTCAATCCTGATGAGGTAACCTTTGGAATCATGATTGGCTGGAGCTGTCGTGAACGAAAACTTAAAAGTGCCTTTGTTTACCTATCAGAGATGTTGGGAAGACACCTAAACCCCCATACATGTACCTATAATGCTCTTATCAGCGGGGTCTTTATGGAGGGTATGTGGAAGCATGCTGGGAAAGTCTTTGATGAGATGGTAGATAGGGGGACTACGCCTGATTTGTCGACTTTCAGGATTCTCTTAGCAGGCTATTGTAAAGCTAGACAATTTGATGAAGCCAAAAGGATAGTGTTTGATATGGCAAGCCATGGACTGATTCAAAATTCTTCAGATGAAGATCCACTATCCAAAGCATTTATGGTTCTGGGTTTCAAGCCATTAGCTGTGACATTGAAAAGAGATAATGATGTAGGGTTTGCTAAAACGGAGTTTTATGATGATCTTGGGAATGGACTTTATTTGGATACGGACCTGGATGAGTATGAGAAAAGAGTCACTGGAATCCTAGAAGATTGCATGGTACCTGATTATTACTCTCTTATGATGATAGAATGCACTCGTGGAAATTTGAAGGGTGCTTTGGTTTTGGCAGATGAAATGATCCGGTGGGGACAAGATTTGTCATTGTCTATGATGTCCGACTTACTGAAAGGGCTTTCTGCATCCCATTTACATAACAAGTTAATCACCAGCATTGTAGATAAAAAGCTTCACTTGGTAAATCAGCTAGACCAAGAAACTCTAAACTTTCTTGCCCAAGCATACGGCAAGAAAGGATTGACATATAATACAAGGATAGTGGTGAATGGAATGATAGAAAGGCATCTGAAAATCAACAATGAGACTTACACTGCTTTAGTAAAAGGTTTTTGTAAGAAAGGAAACTTGAGGGAGATGAATGCTTGCTGGAATACTGCTCAAAATGACGGATGGTTACCAGTGCCGGAAGATTGTAAAGCCTTGATTGAATGTCTTTTCCATAAAGAAATGCTAAGAGAAGCAGTGCAGCTTCTTGAAAGCATTCTGATATCCTACCCAGACTTAAGGTCAGATATGTGTCATATGATCCTTGACAAGCTTTTTGTTACCGGTTGTACAGGAATTGCAAGCATATTGTTAGAAGAACTTGAACAGCGTGGTGGCATCCTGGATCAGATGGCTTATAACAATATTATTAGAGGATTGTGTAAGGGCAAGAATTTTCATGTGGCCTTTACAGTGCTGGACAGTATGCTGGCTAAAAATTTGGCCCCTTGTTTGGATGTTACTGTCCAATTAATTCCTCAGTTGTGTAGGGCTGATAGATTTGGAAAAGCAGTTAACTTGAAAGAGATTGGTCTAAGAGAGAACTCTTCCTATTCACTTTCTCTAGACCGTGCATTGATAGAAGGCTGTTGTATTTCAGGGAAAGTTATAGAAGCAACCACTCTACTACAGAATATGTTGTTAAAGCGGATACCTCCTGATGCTGAAATTTACAATTTTCTGGTTCAGGGTCATTGCAAGGTTAATGACTTAAAGAAAGTTTGGGAGCTACTTTGTGTCATGACAAGAAAAGGTTTCGACATTTCACTCTCAACTTACCGCAACTTGGTGTGTTTGATGTGTCTGGAAGGTAGGGTTCTTCATGCATGGAAATTGACTGAGCTTATGATTGGACAAAGTGATCCTCATGATCTCAGCATCTACAATATTCTGATATTCTATATTTTCCCAACCGGGAATACTTTACTTGTCAAGAAAGTTGTGGAGCATTTGCAAGAGAAGAAATTGCTACTTGATGAAGTAACTTACAACTTTCTTGTACTTGGGTTTTGTAGATCTAAAGATGTGTCGAGTGCTGTGGATCATCTGTACACTATGATCTCTAAGGATTTCAGGCCAAGCAACCGCAACTTGAGAAAAGTTATAACCAGCCTGTGTGATGTTGGGGAGATTGAGAAAGCCTTGGAACTGAGTCGAGAAATGGAATTGAGAGGCCAGATTCATGATTCAATGATTCAAAATGCGATTGTTGAGGGTCTTTCATCTCTTGGTAGGATTCAAGAAGCTGAAAGTTTTCTGGACAGGATGGAAGAGAAATGTCTAATTCCAGAAAATGTCAACTATAATAATCTAATCAAACTGTTTTGCTCATATGGAAGACCAAACAAGGCAGTTAATTCTCTGAACATAATGCTGAAGAAAGGGAATGTTCCAAATTCTACCAGTTTTGATTCCATCATCAGCTCTTTCTGTGCCCTTGGTAATCTGGAGCAAGCTATGGATTTTCATGCTGAGATGTTGGATAGAAATCTTAAGCCAAGCATCGACACTTGGGACATTCTGGTCCATAACTTTTGTCAAGATGGAAAAACAGCAGAGGCAGAAAGGCTATTGAAGTCTATGGGTTGTGCAGGTGAAACAGTGACCAGGAAGATATATCTGTCTGTAATTAATAGTTATCGCTCAGAGAACAATATCGGAAAGGTCTCGGAGCTCATGCAAGCAATGCAACAGAGTGGTTATGAGCCAGACTTCGAGACACATTGGTCTCTCATAAGGAACCTAAGAGTTTCTAGTGACAAGGACACTGCAAACAGCAGCGAGGGTTTCCTGTCAAAGCTTCTCTCTGCAAGTGGATTTTCTAGGCAAAAGTATTCCAAGGCCAAACAGGGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0048037 GO:0051536 GO:0051540 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2

Protein Analysis

1266

Amino Acids

142.87

Weight (kDa)

6.56

Isoelectric Point (pI)

35.72

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	381 - 427	2.9e-07	PPR repeat family
PPR_2	PF13041	416 - 465	5.1e-14	PPR repeat family
PPR_2	PF13041	867 - 915	1.7e-07	PPR repeat family
TPR_24	PF23276	953 - 1064	5.4e-09	Fungal tetratrico peptide repeats
PPR_2	PF13041	1078 - 1125	4e-09	PPR repeat family
PPR_2	PF13041	1112 - 1161	2.6e-09	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0002362)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G15280
fragaria_vesca	FvH4_6g47953 FvH4_6g48150 FvH4_6g48150 FvH4_6g48150 FvH4_6g48150 FvH4_6g48150 FvH4_6g48880 FvH4_6g48880
malus_domestica	MD09G1053400.v1.1
prunus_persica	Prupe.3G265200_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0167491 RchiOBHm_Chr2g0167571 RchiOBHm_Chr2g0167581
rosa_laevigata	RLG00000021724 RLG00000021736
rosa_multiflora	Rmu_sc0000056.1_g000010 Rmu_sc0000056.1_g000011 Rmu_sc0001970.1_g000002
rosa_roxburghii	Rroxscaffold_2G00083370 Rroxscaffold_2G00083410 Rroxscaffold_2G00083440
rosa_rugosa	Rorug02G0530600
rosa_samantha	Rh2AG597900 Rh2AG598800 Rh2AG599200 Rh2AG599300 Rh2AG599400 Rh2BG609400 Rh2BG609800 Rh2BG609900 Rh2BG610000 Rh2CG580300 Rh2DG620700 Rh2DG621000 Rh2DG621100 Rh2DG621900 Rh2DG622000 Rh2DG622100 Rh2DG622600 Rh2DG622700
rosa_wichuraiana	Rw2G049750

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	2301, 3047
AarI	CACCTGC	2 cut(s)	68, 3524
AasI	GACNNNNNNGTC	2 cut(s)	3464, 3667
Acc16I	TGCGCA	1 cut(s)	429
Acc36I	ACCTGC	2 cut(s)	68, 3524
Acc65I	GGTACC	1 cut(s)	1657
AccB1I	GGYRCC	1 cut(s)	1657
AccI	GTMKAC	1 cut(s)	1361
AciI	CCGC	5 cut(s)	364, 1278, 2590, 2722, 3028
AclWI	GGATC	6 cut(s)	1469, 1732, 2197, 2295, 2798, 2991
AcuI	CTGAAG	2 cut(s)	1449, 3338
AfaI	GTAC	6 cut(s)	56, 1258, 1659, 2233, 2943, 2993
AfiI	CCNNNNNNNGG	4 cut(s)	319, 2395, 2728, 3792
AflII	CTTAAG	2 cut(s)	2179, 3434
AflIII	ACRYGT	1 cut(s)	1253
AgeI	ACCGGT	1 cut(s)	2225
AhdI	GACNNNNNGTC	1 cut(s)	2182
AjnI	CCWGG	3 cut(s)	1608, 2283, 3548
AjuI	GAANNNNNNNTTGG	6 cut(s)	764, 771, 796, 803, 1700, 1732
Alw21I	GWGCWC	1 cut(s)	3618
Alw26I	GTCTC	5 cut(s)	3, 1973, 3613, 3653, 3675
AlwI	GGATC	6 cut(s)	1469, 1732, 2197, 2295, 2798, 2991
AoxI	GGCC	8 cut(s)	537, 681, 843, 2346, 2385, 3017, 3115, 3783
ApeKI	GCWGC	2 cut(s)	2143, 3720
ArsI	GACNNNNNNTTYG	2 cut(s)	2201, 2233
AseI	ATTAAT	2 cut(s)	2414, 3573
AsiGI	ACCGGT	1 cut(s)	2225
Asp700I	GAANNNNTTC	4 cut(s)	42, 992, 2157, 3195
Asp718I	GGTACC	1 cut(s)	1657
AspLEI	GCGC	1 cut(s)	430
AspS9I	GGNCC	4 cut(s)	681, 1606, 2386, 3466
AsuC2I	CCSGG	1 cut(s)	2857
AsuHPI	GGTGA	7 cut(s)	115, 165, 184, 680, 1816, 1951, 3548
AsuII	TTCGAA	1 cut(s)	930
AvaII	GGWCC	2 cut(s)	1606, 3466
BaeGI	GKGCMC	2 cut(s)	1036, 3379
BanI	GGYRCC	1 cut(s)	1657
BanII	GRGCYC	1 cut(s)	3618
BauI	CACGAG	1 cut(s)	1695
BbsI	GAAGAC	4 cut(s)	453, 779, 1240, 3286
Bbv12I	GWGCWC	1 cut(s)	3618
BbvI	GCAGC	2 cut(s)	2155, 3732
BccI	CCATC	9 cut(s)	197, 730, 875, 1324, 2061, 2287, 3202, 3365, 3479
BceAI	ACGGC	1 cut(s)	1918
BciT130I	CCWGG	3 cut(s)	1610, 2285, 3550
BciVI	GTATCC	2 cut(s)	1594, 2586
BcnI	CCSGG	1 cut(s)	2857
BcoDI	GTCTC	5 cut(s)	3, 1973, 3613, 3653, 3675
BfaI	CTAG	8 cut(s)	447, 942, 1398, 1643, 1865, 2507, 3696, 3765
BfmI	CTRYAG	1 cut(s)	2570
BfrI	CTTAAG	2 cut(s)	2179, 3434
BfuAI	ACCTGC	2 cut(s)	68, 3524
BfuI	GTATCC	2 cut(s)	1594, 2586
BglII	AGATCT	1 cut(s)	2957
BisI	GCNGC	2 cut(s)	2144, 3721
BlsI	GCNGC	2 cut(s)	2145, 3722
Bme1390I	CCNGG	4 cut(s)	1610, 2285, 2857, 3550
Bme18I	GGWCC	2 cut(s)	1606, 3466
BmeRI	GACNNNNNGTC	1 cut(s)	2182
BmgT120I	GGNCC	4 cut(s)	681, 1606, 2386, 3466
BmiI	GGNNCC	4 cut(s)	375, 1659, 2388, 3683
BmrFI	CCNGG	4 cut(s)	1610, 2285, 2857, 3550
BmsI	GCATC	7 cut(s)	534, 1808, 1969, 2289, 2593, 2829, 3453
BpiI	GAAGAC	4 cut(s)	453, 779, 1240, 3286
BpmI	CTGGAG	2 cut(s)	1195, 3410
Bpu10I	CCTNAGC	1 cut(s)	359
Bpu14I	TTCGAA	1 cut(s)	930
BpuEI	CTTGAG	3 cut(s)	920, 2044, 3055
BpuMI	CCSGG	1 cut(s)	2857
BsaBI	GATNNNNATC	2 cut(s)	1473, 3429
BsaI	GGTCTC	2 cut(s)	3613, 3675
BsaJI	CCNNGG	4 cut(s)	1444, 3084, 3379, 3780
BsaWI	WCCGGW	2 cut(s)	1740, 2225
BsaXI	ACNNNNNCTCC	4 cut(s)	1168, 1198, 1283, 1313
Bsc4I	CCNNNNNNNGG	4 cut(s)	319, 2395, 2728, 3792
Bse118I	RCCGGY	1 cut(s)	2225
Bse1I	ACTGG	3 cut(s)	1639, 2075, 3345
Bse3DI	GCAATG	4 cut(s)	40, 725, 2635, 3633
Bse8I	GATNNNNATC	2 cut(s)	1473, 3429
BseBI	CCWGG	3 cut(s)	1610, 2285, 3550
BseDI	CCNNGG	4 cut(s)	1444, 3084, 3379, 3780
BseGI	GGATG	8 cut(s)	158, 886, 1618, 1799, 2072, 2280, 2404, 3213
BseJI	GATNNNNATC	2 cut(s)	1473, 3429
BseLI	CCNNNNNNNGG	4 cut(s)	319, 2395, 2728, 3792
BseMI	GCAATG	4 cut(s)	40, 725, 2635, 3633
BseMII	CTCAG	8 cut(s)	533, 1011, 2435, 2772, 2830, 3083, 3405, 3600
BseNI	ACTGG	3 cut(s)	1639, 2075, 3345
BseSI	GKGCMC	2 cut(s)	1036, 3379
BseXI	GCAGC	2 cut(s)	2155, 3732
BseYI	CCCAGC	2 cut(s)	1049, 1310
BsgI	GTGCAG	2 cut(s)	2162, 3552
BshFI	GGCC	8 cut(s)	539, 683, 845, 2348, 2387, 3019, 3117, 3785
BshNI	GGYRCC	1 cut(s)	1657
BshTI	ACCGGT	1 cut(s)	2225
BsiHKAI	GWGCWC	1 cut(s)	3618
BsiSI	CCGG	4 cut(s)	1741, 2081, 2226, 2856
BslFI	GGGAC	4 cut(s)	1102, 1357, 1761, 3470
BslI	CCNNNNNNNGG	4 cut(s)	319, 2395, 2728, 3792
BsmAI	GTCTC	5 cut(s)	3, 1973, 3613, 3653, 3675
BsmFI	GGGAC	4 cut(s)	1102, 1357, 1761, 3470
BsmI	GAATGC	4 cut(s)	1066, 1694, 2041, 2157
BsnI	GGCC	8 cut(s)	539, 683, 845, 2348, 2387, 3019, 3117, 3785
Bso31I	GGTCTC	2 cut(s)	3613, 3675
Bsp119I	TTCGAA	1 cut(s)	930
Bsp1286I	GDGCHC	3 cut(s)	1036, 3379, 3618
Bsp1407I	TGTACA	3 cut(s)	54, 2231, 2991
Bsp19I	CCATGG	1 cut(s)	1444
BspACI	CCGC	5 cut(s)	364, 1278, 2590, 2722, 3028
BspANI	GGCC	8 cut(s)	539, 683, 845, 2348, 2387, 3019, 3117, 3785
BspCNI	CTCAG	8 cut(s)	532, 1012, 2434, 2773, 2829, 3084, 3406, 3599
BspHI	TCATGA	4 cut(s)	1164, 2682, 2809, 3124
BspLI	GGNNCC	4 cut(s)	375, 1659, 2388, 3683
BspMI	ACCTGC	2 cut(s)	68, 3524
BspPI	GGATC	6 cut(s)	1469, 1732, 2197, 2295, 2798, 2991
BspQI	GCTCTTC	1 cut(s)	1090
BspT104I	TTCGAA	1 cut(s)	930
BspT107I	GGYRCC	1 cut(s)	1657
BspTI	CTTAAG	2 cut(s)	2179, 3434
BspTNI	GGTCTC	2 cut(s)	3613, 3675
BsrDI	GCAATG	4 cut(s)	40, 725, 2635, 3633
BsrFI	RCCGGY	1 cut(s)	2225
BsrGI	TGTACA	3 cut(s)	54, 2231, 2991
BsrI	ACTGG	3 cut(s)	1639, 2075, 3345
BssAI	RCCGGY	1 cut(s)	2225
BssECI	CCNNGG	4 cut(s)	1444, 3084, 3379, 3780
BssSI	CACGAG	1 cut(s)	1695
BssT1I	CCWWGG	4 cut(s)	1444, 3084, 3379, 3780
Bst2BI	CACGAG	1 cut(s)	1695
Bst2UI	CCWGG	3 cut(s)	1610, 2285, 3550
Bst4CI	ACNGT	7 cut(s)	638, 2356, 2366, 2407, 2513, 3264, 3544
Bst6I	CTCTTC	4 cut(s)	125, 1090, 2494, 3207
BstAFI	CTTAAG	2 cut(s)	2179, 3434
BstAPI	GCANNNNNTGC	1 cut(s)	3628
BstAUI	TGTACA	3 cut(s)	54, 2231, 2991
BstBI	TTCGAA	1 cut(s)	930
BstC8I	GCNNGC	8 cut(s)	1308, 1384, 1441, 2041, 2245, 2373, 3396, 3624
BstDSI	CCRYGG	1 cut(s)	1444
BstEII	GGTNACC	2 cut(s)	1150, 2070
BstF5I	GGATG	8 cut(s)	158, 886, 1618, 1799, 2072, 2280, 2404, 3213
BstHHI	GCGC	1 cut(s)	430
BstMAI	GTCTC	5 cut(s)	3, 1973, 3613, 3653, 3675
BstMWI	GCNNNNNNNGC	4 cut(s)	427, 718, 727, 3628
BstNI	CCWGG	3 cut(s)	1610, 2285, 3550
BstNSI	RCATGY	2 cut(s)	1257, 1310
BstPI	GGTNACC	2 cut(s)	1150, 2070
BstSCI	CCNGG	4 cut(s)	1608, 2283, 2855, 3548
BstSFI	CTRYAG	1 cut(s)	2570
BstSLI	GKGCMC	2 cut(s)	1036, 3379
BstV1I	GCAGC	2 cut(s)	2155, 3732
BstV2I	GAAGAC	4 cut(s)	453, 779, 1240, 3286
BstX2I	RGATCY	2 cut(s)	1474, 2957
BstYI	RGATCY	2 cut(s)	1474, 2957
BsuI	GTATCC	2 cut(s)	1594, 2586
BsuRI	GGCC	8 cut(s)	539, 683, 845, 2348, 2387, 3019, 3117, 3785
BtgI	CCRYGG	1 cut(s)	1444
BtgZI	GCGATG	1 cut(s)	51
BtsCI	GGATG	8 cut(s)	158, 886, 1618, 1799, 2072, 2280, 2404, 3213
BtsI	GCAGTG	5 cut(s)	428, 737, 1986, 2145, 3708
BveI	ACCTGC	2 cut(s)	68, 3524
Cac8I	GCNNGC	8 cut(s)	1308, 1384, 1441, 2041, 2245, 2373, 3396, 3624
CciI	TCATGA	4 cut(s)	1164, 2682, 2809, 3124
CfoI	GCGC	1 cut(s)	430
Cfr10I	RCCGGY	1 cut(s)	2225
Cfr13I	GGNCC	4 cut(s)	681, 1606, 2386, 3466
Csp6I	GTAC	6 cut(s)	55, 1257, 1658, 2232, 2942, 2992
CspAI	ACCGGT	1 cut(s)	2225
CspCI	CAANNNNNGTGG	2 cut(s)	1917, 1952
CviQI	GTAC	6 cut(s)	55, 1257, 1658, 2232, 2942, 2992
DraI	TTTAAA	1 cut(s)	957
DrdI	GACNNNNNNGTC	2 cut(s)	3464, 3667
DriI	GACNNNNNGTC	1 cut(s)	2182
DseDI	GACNNNNNNGTC	2 cut(s)	3464, 3667
Eam1104I	CTCTTC	4 cut(s)	125, 1090, 2494, 3207
Eam1105I	GACNNNNNGTC	1 cut(s)	2182
EarI	CTCTTC	4 cut(s)	125, 1090, 2494, 3207
Ecl136II	GAGCTC	1 cut(s)	3616
Eco130I	CCWWGG	4 cut(s)	1444, 3084, 3379, 3780
Eco24I	GRGCYC	1 cut(s)	3618
Eco31I	GGTCTC	2 cut(s)	3613, 3675
Eco32I	GATATC	1 cut(s)	2166
Eco47I	GGWCC	2 cut(s)	1606, 3466
Eco53kI	GAGCTC	1 cut(s)	3616
Eco57I	CTGAAG	2 cut(s)	1449, 3338
Eco91I	GGTNACC	2 cut(s)	1150, 2070
EcoICRI	GAGCTC	1 cut(s)	3616
EcoO65I	GGTNACC	2 cut(s)	1150, 2070
EcoRII	CCWGG	3 cut(s)	1608, 2283, 3548
EcoRV	GATATC	1 cut(s)	2166
EcoT14I	CCWWGG	4 cut(s)	1444, 3084, 3379, 3780
EcoT22I	ATGCAT	2 cut(s)	1068, 2770
EcoT38I	GRGCYC	1 cut(s)	3618
ErhI	CCWWGG	4 cut(s)	1444, 3084, 3379, 3780
FaqI	GGGAC	4 cut(s)	1102, 1357, 1761, 3470
FauI	CCCGC	2 cut(s)	357, 1271
FauNDI	CATATG	2 cut(s)	2199, 3274
FblI	GTMKAC	1 cut(s)	1361
Fnu4HI	GCNGC	2 cut(s)	2144, 3721
FokI	GGATG	8 cut(s)	145, 893, 1625, 1786, 2079, 2267, 2411, 3220
FriOI	GRGCYC	1 cut(s)	3618
Fsp4HI	GCNGC	2 cut(s)	2144, 3721
FspBI	CTAG	8 cut(s)	447, 942, 1398, 1643, 1865, 2507, 3696, 3765
FspI	TGCGCA	1 cut(s)	429
GlaI	GCGC	1 cut(s)	429
GluI	GCNGC	2 cut(s)	2144, 3721
GsaI	CCCAGC	2 cut(s)	1053, 1314
GsuI	CTGGAG	2 cut(s)	1195, 3410
HaeIII	GGCC	8 cut(s)	539, 683, 845, 2348, 2387, 3019, 3117, 3785
HapII	CCGG	4 cut(s)	1741, 2081, 2226, 2856
HhaI	GCGC	1 cut(s)	430
Hin6I	GCGC	1 cut(s)	428
HinP1I	GCGC	1 cut(s)	428
HincII	GTYRAC	3 cut(s)	1362, 2458, 3241
HindII	GTYRAC	3 cut(s)	1362, 2458, 3241
HindIII	AAGCTT	3 cut(s)	1844, 2213, 3740
HpaI	GTTAAC	1 cut(s)	2458
HpaII	CCGG	4 cut(s)	1741, 2081, 2226, 2856
HphI	GGTGA	7 cut(s)	115, 165, 184, 680, 1816, 1951, 3548
Hpy166II	GTNNAC	7 cut(s)	57, 1187, 1213, 1362, 2458, 2994, 3241
Hpy8I	GTNNAC	7 cut(s)	57, 1187, 1213, 1362, 2458, 2994, 3241
HpyAV	CCTTC	7 cut(s)	323, 835, 910, 982, 1702, 2519, 2744
HpyCH4III	ACNGT	7 cut(s)	638, 2356, 2366, 2407, 2513, 3264, 3544
HpyF10VI	GCNNNNNNNGC	4 cut(s)	427, 718, 727, 3628
HspAI	GCGC	1 cut(s)	428
KpnI	GGTACC	1 cut(s)	1661
KspAI	GTTAAC	1 cut(s)	2458
LguI	GCTCTTC	1 cut(s)	1090
LmnI	GCTCC	6 cut(s)	373, 1176, 2668, 2887, 3391, 3613
Lsp1109I	GCAGC	2 cut(s)	2155, 3732
LweI	GCATC	7 cut(s)	534, 1808, 1969, 2289, 2593, 2829, 3453
MaeI	CTAG	8 cut(s)	447, 942, 1398, 1643, 1865, 2507, 3696, 3765
MfeI	CAATTG	2 cut(s)	755, 779
MflI	RGATCY	2 cut(s)	1474, 2957
MhlI	GDGCHC	3 cut(s)	1036, 3379, 3618
MlyI	GAGTC	3 cut(s)	467, 1638, 3103
MmeI	TCCRAC	4 cut(s)	415, 597, 1800, 3402
Mph1103I	ATGCAT	2 cut(s)	1068, 2770
MroXI	GAANNNNTTC	4 cut(s)	42, 992, 2157, 3195
MslI	CAYNNNNRTG	1 cut(s)	730
MspA1I	CMGCKG	1 cut(s)	1278
MspCI	CTTAAG	2 cut(s)	2179, 3434
MspI	CCGG	4 cut(s)	1741, 2081, 2226, 2856
MspR9I	CCNGG	4 cut(s)	1610, 2285, 2857, 3550
MunI	CAATTG	2 cut(s)	755, 779
Mva1269I	GAATGC	4 cut(s)	1066, 1694, 2041, 2157
MvaI	CCWGG	3 cut(s)	1610, 2285, 3550
MwoI	GCNNNNNNNGC	4 cut(s)	427, 718, 727, 3628
NciI	CCSGG	1 cut(s)	2857
NcoI	CCATGG	1 cut(s)	1444
NdeI	CATATG	2 cut(s)	2199, 3274
NlaIV	GGNNCC	4 cut(s)	375, 1659, 2388, 3683
NmuCI	GTSAC	4 cut(s)	1522, 1630, 3544, 3698
NsbI	TGCGCA	1 cut(s)	429
NsiI	ATGCAT	2 cut(s)	1068, 2770
NspI	RCATGY	2 cut(s)	1257, 1310
NspV	TTCGAA	1 cut(s)	930
PaeI	GCATGC	1 cut(s)	1310
PagI	TCATGA	4 cut(s)	1164, 2682, 2809, 3124
PaqCI	CACCTGC	2 cut(s)	68, 3524
PciI	ACATGT	1 cut(s)	1253
PciSI	GCTCTTC	1 cut(s)	1090
PctI	GAATGC	4 cut(s)	1066, 1694, 2041, 2157
PdmI	GAANNNNTTC	4 cut(s)	42, 992, 2157, 3195
PfoI	TCCNGGA	1 cut(s)	2283
PinAI	ACCGGT	1 cut(s)	2225
PkrI	GCNGC	2 cut(s)	2145, 3722
PleI	GAGTC	3 cut(s)	466, 1637, 3102
PpsI	GAGTC	3 cut(s)	466, 1637, 3102
PscI	ACATGT	1 cut(s)	1253
PshBI	ATTAAT	2 cut(s)	2414, 3573
PsiI	TTATAA	2 cut(s)	2301, 3047
Psp124BI	GAGCTC	1 cut(s)	3618
Psp6I	CCWGG	3 cut(s)	1608, 2283, 3548
PspEI	GGTNACC	2 cut(s)	1150, 2070
PspFI	CCCAGC	2 cut(s)	1049, 1310
PspGI	CCWGG	3 cut(s)	1608, 2283, 3548
PspN4I	GGNNCC	4 cut(s)	375, 1659, 2388, 3683
PspPI	GGNCC	4 cut(s)	681, 1606, 2386, 3466
PsuI	RGATCY	2 cut(s)	1474, 2957
RsaI	GTAC	6 cut(s)	56, 1258, 1659, 2233, 2943, 2993
RsaNI	GTAC	6 cut(s)	55, 1257, 1658, 2232, 2942, 2992
RseI	CAYNNNNRTG	1 cut(s)	730
SacI	GAGCTC	1 cut(s)	3618
SalI	GTCGAC	1 cut(s)	1360
SapI	GCTCTTC	1 cut(s)	1090
SatI	GCNGC	2 cut(s)	2144, 3721
Sau96I	GGNCC	4 cut(s)	681, 1606, 2386, 3466
SchI	GAGTC	3 cut(s)	467, 1638, 3103
ScrFI	CCNGG	4 cut(s)	1610, 2285, 2857, 3550
SduI	GDGCHC	3 cut(s)	1036, 3379, 3618
SfaNI	GCATC	7 cut(s)	534, 1808, 1969, 2289, 2593, 2829, 3453
SfcI	CTRYAG	1 cut(s)	2570
SfuI	TTCGAA	1 cut(s)	930
SinI	GGWCC	2 cut(s)	1606, 3466
SmiMI	CAYNNNNRTG	1 cut(s)	730
SmlI	CTYRAG	5 cut(s)	935, 2023, 2179, 3034, 3434
SmoI	CTYRAG	5 cut(s)	935, 2023, 2179, 3034, 3434
SphI	GCATGC	1 cut(s)	1310
SsiI	CCGC	5 cut(s)	364, 1278, 2590, 2722, 3028
SspI	AATATT	3 cut(s)	263, 2308, 2830
SspMI	CTAG	8 cut(s)	447, 942, 1398, 1643, 1865, 2507, 3696, 3765
SstI	GAGCTC	1 cut(s)	3618
StyD4I	CCNGG	4 cut(s)	1608, 2283, 2855, 3548
StyI	CCWWGG	4 cut(s)	1444, 3084, 3379, 3780
TaaI	ACNGT	7 cut(s)	638, 2356, 2366, 2407, 2513, 3264, 3544
TatI	WGTACW	4 cut(s)	54, 2231, 2941, 2991
TseFI	GTSAC	4 cut(s)	1522, 1630, 3544, 3698
TseI	GCWGC	2 cut(s)	2143, 3720
Tsp45I	GTSAC	4 cut(s)	1522, 1630, 3544, 3698
TspGWI	ACGGA	3 cut(s)	1577, 1619, 2079
Vha464I	CTTAAG	2 cut(s)	2179, 3434
VpaK11BI	GGWCC	2 cut(s)	1606, 3466
VspI	ATTAAT	2 cut(s)	2414, 3573
XbaI	TCTAGA	1 cut(s)	2506
XceI	RCATGY	2 cut(s)	1257, 1310
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1492
XmiI	GTMKAC	1 cut(s)	1361
XmnI	GAANNNNTTC	4 cut(s)	42, 992, 2157, 3195
XspI	CTAG	8 cut(s)	447, 942, 1398, 1643, 1865, 2507, 3696, 3765
Zsp2I	ATGCAT	2 cut(s)	1068, 2770

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.