Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2B

Orthogroup ID

OG0002362

Physical Location & Seq

Genomic Coordinates Reverse (-)

83063710 .. 83067477

Gene Structure 3768 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2BG609400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3768 bp

ATGCTTCAAGTTTTGTGTACCTGTTCACCCCACAAGCCTCACATCAAACAGGTTGGTTCTCTTTACTCTCTGTTCTCACTCTTCACCAATAACCACTGCCTCTCAACTACCACTTCTTCATCCCCAATTCCCCATCATCTTCAACAACACACAAAAACCAACACCCAAGTAGATTTATCTCGTAATTGTTTCAATGGTATAGCCCAATCTGTTATATTGAGGTGTTCCCAATATTTTGACAAGAGTAAGGGCAAAGACTTTGCCAATGCTTCTCTAAAAGACCTTCTTTTGGAGATTTCTGATTTAGTGCCTGAACAAACTCGTAGGCTTAGGCGGGTTTCGGAGCCGAAACCCGAAGATGTGCTTGAATTATTACTGGGTTTTGAATTGCAGTGTGCGCAAGTTGGATTTGAAGCTAGAAAGGTTGAGTCTTTGTGGGGAATTTTCAAGTGGGTTAGTGAAAAAGTTGAGGGCTTTAAGCATAAACCTCAGTCATGTGAGGTCATGGCCTCGTTGCTTCTCCGAGTGGGATTGCTTAGAGAAGTTGAGTTCTTGCTTTCCACAATGGAAAGTCAAGGAGTTTTGTTGGATAGTCAAGAAATTTACAGTGATTTGATTAAAGGGTATGTTGGTGTTGGTGAGTTAGATAGGGCCATTTCTGTCTATGATCGAATTACGGGGCGTGTAGTGCCATCATTGCAGTGTTGTTGTGTTCTACTTGATCAATTGGTGGGAATGAGGAAGACCAAATTGGCGTTTCGAGTTTGTAGTGATATGGCGGAAATGGGTTTTGATTTGAGAGATGTGAAGAAGGCCACATTTGAGGGTCTCGTTAGACTGCTTTGCAGGGATGGAAAGATTCAGGAGGCAAGAAATTTTGTCAAGAAGGCTATGGCTTTCGAACTCAAGCCTAGCAACTTAGTTTTAAATGAAGTTGCTTATGGATATTGTGAGAAGAAGGACTTTGATGATTTGATGAGCTTCTATTCTGAGATTAAGTGTGCCCCAGAAGTTATGGCTGGGAATAGGATTATGCATTCTCTTTGTAGCCATTTTGGGACAAGAAGAGCAGAACCTTATTTGCAAGAATTAGAAATTTTAGGCTTCAATCCTGATGAGGTAACCTTTGGAATCATGATTGGCTGGAGCTGTCGTGAACGAAAACTTAAAAGTGCCTTTGTTTACCTATCAGAGATGTTGGGAAGACACCTAAACCCCCATATATGCACCTATAATGCTCTTATCAGCGGGGTATTTATGGAGGGTATGTGGAAGCACGCTGGGAAGGTGTTTGATGAGATGGTAGAGAGGGGGACTACACCTGATTTGTCGACTTTCAGGATTCTCTTAGCCGGCTATTGTAAAGTCAGAAATTTTGATGAAGCCAAAAGGATAGTTTTTGATATGGCAAGCCATGGACTGATTCAAAACTCGTCAGATGAGGATCCGCTATCCAAAGCATTTATGGTTCTGGGTTTCAAGCCATTAGCTGTGACATTGAAGAGAGATAATGATGTTGGGTTTGCTAAAACAGAGTTTTATGATGATCTTGGGAATGGACTTTATTTGGATACAGACCTGGATAAGTATGAGAAAAGAGTCACTGGGATCCTAGAAGATTGCATGGTACCTGATTATTACTCTCTTATGATGATAGAATGCAGTCGTAGAAATTTGAAGGGTGCTTTGGTTTTGGCAGATGAAATAATCCGGTGGGGACAAGATTTGTCATTGTCTATGATGTCCGACTTACTGAAAGGGCTTTCTGCATCCCATTTACATACCAAGGGTATCACCAGCATTGTAGATAAAAAGCTTCACTTGGTAAATCAGCTAGACCAAGAAACTCTAAACTTTCTTGCCCAAGCATACGGCAAGAAAGGATTGACATATAATACAAGGATAGTGGTGAATGGAATGATAGAAAGGCATCTGAAAATCAACAATGAGACTTACACTGCTTTAGTAAAAGGTTTTTGTAAGAAAGGAAACTTGAGGGAGCTGAATGCTTGCTGGAATATTGCTCAAAATGACGGATGGTTACCAGGGCCGGAAGATAGTAAAGCCTTGATTGAATGTCTTTTCCATAAAGAAATGCTGAGAGAAGCAGTGCAGCTTCTTGAAAGCATTCTGATATCCTACCCAGACTTAAGGTCAGATATGTGTCATATGATCCTTGACAAGCTTTTTGTTACCGGTTGTACAGGAATTGCAAGCATATTGTTAGGAGAACTTGAACAGCGTGGTGGCATCCTGGATCAGATGGCTTATAACAATATTATTAGAGGATTGTGTAAGGGCAAGAATTTTCATGTGGCCTTTAAAGTGCTGGACAGTATGCTGGCTAAAAATTTGGCCCCTTGTTTGGATGTTACTGTCCAATTAATTCCTCAGTTGTGTAGGGCTGATAGATTTGGAAAAGCAGTTAACTTGAAAGAGATTGGTCTAAGAGTGAACTCTTCCTATTCACTTTCTCTAGATCGTGCATTGATAAAAGGCTGTTGTATTTCAGGGAAAGTTACAGAAGCAACCACTCTACTACAGAATATGTTGTTAAAGCGGATACCTCCTGATGCTGAAATTTACAATTTTCTGGTTCAGGGTCATTGCAAGGTTAATGACTTAAAGAAAGTTTGGGAGCTACTTTGTGTCATGACAAGAAAAGGTTTCAACATTTCACTCTCAACTTACCGCAACTTGGTGTGTTTGATGTGTCTGGAAGGTAGGGTTCTTCATGCATGGAAATTGACTGAGCTTATGATTGGACAAAGTGATCCTCATGATCTCAGCATCTACAATATTCTGATATTCTATATTTTCCCAACCGGGAATACTTTGCTTGTCAAGAAAGTTGTGGAGCATTTGCAAGAGAAGAAATTGCTACTTGATGAAGTAACTTACAACTTTCTTGTACTTGGGTTTTGTAGATCTAAAGATGTGTCGAGTGCTGTGGATCATCTGTACACTATGATCTCTAAGGATTTCAGGCCAAGCAACCGCAACTTGAGAAAAGTTATAACCAGCCTGTGTGATGTTGGGGAGATTGAGAAAGCCTTGGAACTGAGTCGAGAAATGGAATTGAGAGGCCAGATTCATGATTCAATGATTCAAAATGCGATTGTTGAGGGTCTTTCTTCTCGTGGTAGGATTCAAGAAGCTGAAAGTTTTCTGGACAGGATGGTAGAGAAATGTCTAATTCCAGAAAATGTCAACTATAATAATCTAATCAAACTGTTTTGCTCGTATGGAAGACCAAACAAGGCAGTTAATTCTCTGAACATAATGCTGAAGAAAGGGAATGTTCCAGATTCTACCAGTTTTGATTCCATCATCAGCTCTTTCTGTGCCCTTGGTAATCTGGAGCAAGCTATGGATTTTCATGCTGAGATGTTGGATAGAAATCTTAAGCCAAGCATCGACACTTGGGACATTCTGGTCCATAACTTTTGTCAAGATGGAAAAACAGCAGAGGCAGAAAGGCTATTGAAGTCTATGGGTTGTGCAGGTGAAACAGTGACCAGGAAGATATATCTGTCTGTAATTAATAGTTATCGCTCAGAGAACAATCTCGGAAAGGTCTCGGAGCTCATGCAAGCAATGCAACAGAGTGGTTATGAGCCAGACTTCGAGACACATTGGTCTCTCATAAGGAACCTAAGAGTTTCTAGTGACAAGGACAATGCAAACAGCAGCAAGGGTTTCCTGTCAAAGCTTCTCTCTGCAAGTGGATTTTCTAGGCAAAAATATTCCAAGGCCAAACAGGGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0048037 GO:0051536 GO:0051540 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2

Protein Analysis

1255

Amino Acids

141.84

Weight (kDa)

7.95

Isoelectric Point (pI)

34.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_3	PF13812	361 - 416	7.7e-06	Pentatricopeptide repeat domain
PPR_2	PF13041	371 - 417	3.6e-07	PPR repeat family
PPR_2	PF13041	406 - 455	3.4e-14	PPR repeat family
PPR_2	PF13041	857 - 905	1.8e-07	PPR repeat family
TPR_24	PF23276	943 - 1054	1.7e-08	Fungal tetratrico peptide repeats
PPR_2	PF13041	1068 - 1115	4.6e-09	PPR repeat family
PPR_2	PF13041	1102 - 1151	2.6e-09	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0002362)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G15280
fragaria_vesca	FvH4_6g47953 FvH4_6g48150 FvH4_6g48150 FvH4_6g48150 FvH4_6g48150 FvH4_6g48150 FvH4_6g48880 FvH4_6g48880
malus_domestica	MD09G1053400.v1.1
prunus_persica	Prupe.3G265200_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0167491 RchiOBHm_Chr2g0167571 RchiOBHm_Chr2g0167581
rosa_laevigata	RLG00000021724 RLG00000021736
rosa_multiflora	Rmu_sc0000056.1_g000010 Rmu_sc0000056.1_g000011 Rmu_sc0001970.1_g000002
rosa_roxburghii	Rroxscaffold_2G00083370 Rroxscaffold_2G00083410 Rroxscaffold_2G00083440
rosa_rugosa	Rorug02G0530600
rosa_samantha	Rh2AG597900 Rh2AG598800 Rh2AG599200 Rh2AG599300 Rh2AG599400 Rh2BG609400 Rh2BG609800 Rh2BG609900 Rh2BG610000 Rh2CG580300 Rh2DG620700 Rh2DG621000 Rh2DG621100 Rh2DG621900 Rh2DG622000 Rh2DG622100 Rh2DG622600 Rh2DG622700
rosa_wichuraiana	Rw2G049750

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	2271, 3017
AarI	CACCTGC	1 cut(s)	3494
AasI	GACNNNNNNGTC	2 cut(s)	3434, 3637
Acc16I	TGCGCA	1 cut(s)	399
Acc36I	ACCTGC	1 cut(s)	3494
Acc65I	GGTACC	1 cut(s)	1627
AccB1I	GGYRCC	1 cut(s)	1627
AccI	GTMKAC	1 cut(s)	1331
AciI	CCGC	7 cut(s)	334, 779, 1248, 1448, 2560, 2692, 2998
AclWI	GGATC	8 cut(s)	1439, 1452, 1603, 1616, 2167, 2265, 2768, 2961
AcsI	RAATTY	9 cut(s)	441, 600, 874, 1095, 1372, 1672, 2305, 2350, 2580
AcuI	CTGAAG	1 cut(s)	3308
AfaI	GTAC	5 cut(s)	19, 1629, 2203, 2913, 2963
AfiI	CCNNNNNNNGG	4 cut(s)	289, 2365, 2698, 3762
AflII	CTTAAG	2 cut(s)	2149, 3404
AgeI	ACCGGT	1 cut(s)	2195
AhdI	GACNNNNNGTC	1 cut(s)	2152
AjnI	CCWGG	4 cut(s)	1578, 2044, 2253, 3518
AjuI	GAANNNNNNNTTGG	4 cut(s)	734, 766, 1670, 1702
Alw21I	GWGCWC	1 cut(s)	3588
Alw26I	GTCTC	5 cut(s)	833, 1943, 3583, 3623, 3645
AlwI	GGATC	8 cut(s)	1439, 1452, 1603, 1616, 2167, 2265, 2768, 2961
AoxI	GGCC	9 cut(s)	507, 651, 813, 2048, 2316, 2355, 2987, 3085, 3753
ApeKI	GCWGC	2 cut(s)	2113, 3690
ApoI	RAATTY	9 cut(s)	441, 600, 874, 1095, 1372, 1672, 2305, 2350, 2580
ArsI	GACNNNNNNTTYG	2 cut(s)	2171, 2203
AseI	ATTAAT	2 cut(s)	2384, 3543
AsiGI	ACCGGT	1 cut(s)	2195
Asp700I	GAANNNNTTC	2 cut(s)	2127, 3165
Asp718I	GGTACC	1 cut(s)	1627
AspLEI	GCGC	1 cut(s)	400
AspS9I	GGNCC	4 cut(s)	651, 2048, 2356, 3436
AsuC2I	CCSGG	1 cut(s)	2827
AsuHPI	GGTGA	6 cut(s)	18, 76, 650, 1786, 1921, 3518
AsuII	TTCGAA	1 cut(s)	900
AvaII	GGWCC	1 cut(s)	3436
BaeGI	GKGCMC	2 cut(s)	1006, 3349
BamHI	GGATCC	2 cut(s)	1444, 1608
BanI	GGYRCC	1 cut(s)	1627
BanII	GRGCYC	1 cut(s)	3588
BauI	CACGAG	1 cut(s)	3138
BbsI	GAAGAC	3 cut(s)	749, 1210, 3256
Bbv12I	GWGCWC	1 cut(s)	3588
BbvI	GCAGC	2 cut(s)	2125, 3702
BccI	CCATC	9 cut(s)	141, 700, 845, 1294, 2031, 2257, 3172, 3335, 3449
BceAI	ACGGC	1 cut(s)	1888
BciT130I	CCWGG	4 cut(s)	1580, 2046, 2255, 3520
BciVI	GTATCC	2 cut(s)	1564, 2556
BclI	TGATCA	1 cut(s)	721
BcnI	CCSGG	1 cut(s)	2827
BcoDI	GTCTC	5 cut(s)	833, 1943, 3583, 3623, 3645
BfaI	CTAG	7 cut(s)	417, 912, 1613, 1835, 2477, 3666, 3735
BfmI	CTRYAG	1 cut(s)	2540
BfrI	CTTAAG	2 cut(s)	2149, 3404
BfuAI	ACCTGC	1 cut(s)	3494
BfuI	GTATCC	2 cut(s)	1564, 2556
BglII	AGATCT	1 cut(s)	2927
BisI	GCNGC	2 cut(s)	2114, 3691
BlsI	GCNGC	2 cut(s)	2115, 3692
Bme1390I	CCNGG	5 cut(s)	1580, 2046, 2255, 2827, 3520
Bme18I	GGWCC	1 cut(s)	3436
BmeRI	GACNNNNNGTC	1 cut(s)	2152
BmgT120I	GGNCC	4 cut(s)	651, 2048, 2356, 3436
BmiI	GGNNCC	6 cut(s)	345, 1446, 1610, 1629, 2358, 3653
BmrFI	CCNGG	5 cut(s)	1580, 2046, 2255, 2827, 3520
BmrI	ACTGGG	2 cut(s)	386, 1614
BmsI	GCATC	6 cut(s)	1778, 1939, 2259, 2563, 2799, 3423
BmuI	ACTGGG	2 cut(s)	386, 1614
BpiI	GAAGAC	3 cut(s)	749, 1210, 3256
BpmI	CTGGAG	2 cut(s)	1165, 3380
Bpu10I	CCTNAGC	1 cut(s)	329
Bpu14I	TTCGAA	1 cut(s)	900
BpuEI	CTTGAG	3 cut(s)	890, 2014, 3025
BpuMI	CCSGG	1 cut(s)	2827
BsaBI	GATNNNNATC	2 cut(s)	1443, 3399
BsaI	GGTCTC	3 cut(s)	833, 3583, 3645
BsaJI	CCNNGG	6 cut(s)	1414, 1785, 2045, 3054, 3349, 3750
BsaWI	WCCGGW	2 cut(s)	1710, 2195
BsaXI	ACNNNNNCTCC	4 cut(s)	1138, 1168, 1253, 1283
Bsc4I	CCNNNNNNNGG	4 cut(s)	289, 2365, 2698, 3762
Bse118I	RCCGGY	2 cut(s)	1352, 2195
Bse1I	ACTGG	3 cut(s)	381, 1609, 3315
Bse3DI	GCAATG	3 cut(s)	695, 2605, 3603
Bse8I	GATNNNNATC	2 cut(s)	1443, 3399
BseBI	CCWGG	4 cut(s)	1580, 2046, 2255, 3520
BseDI	CCNNGG	6 cut(s)	1414, 1785, 2045, 3054, 3349, 3750
BseGI	GGATG	7 cut(s)	119, 856, 1769, 2042, 2250, 2374, 3183
BseJI	GATNNNNATC	2 cut(s)	1443, 3399
BseLI	CCNNNNNNNGG	4 cut(s)	289, 2365, 2698, 3762
BseMI	GCAATG	3 cut(s)	695, 2605, 3603
BseMII	CTCAG	9 cut(s)	503, 981, 2090, 2405, 2742, 2800, 3053, 3375, 3570
BseNI	ACTGG	3 cut(s)	381, 1609, 3315
BseSI	GKGCMC	2 cut(s)	1006, 3349
BseXI	GCAGC	2 cut(s)	2125, 3702
BseYI	CCCAGC	2 cut(s)	1019, 1280
BsgI	GTGCAG	2 cut(s)	2132, 3522
BshFI	GGCC	9 cut(s)	509, 653, 815, 2050, 2318, 2357, 2989, 3087, 3755
BshNI	GGYRCC	1 cut(s)	1627
BshTI	ACCGGT	1 cut(s)	2195
BsiHKAI	GWGCWC	1 cut(s)	3588
BsiSI	CCGG	5 cut(s)	1353, 1711, 2051, 2196, 2826
BslFI	GGGAC	4 cut(s)	1072, 1327, 1731, 3440
BslI	CCNNNNNNNGG	4 cut(s)	289, 2365, 2698, 3762
BsmAI	GTCTC	5 cut(s)	833, 1943, 3583, 3623, 3645
BsmFI	GGGAC	4 cut(s)	1072, 1327, 1731, 3440
BsmI	GAATGC	4 cut(s)	1036, 1664, 2011, 2127
BsnI	GGCC	9 cut(s)	509, 653, 815, 2050, 2318, 2357, 2989, 3087, 3755
Bso31I	GGTCTC	3 cut(s)	833, 3583, 3645
Bsp119I	TTCGAA	1 cut(s)	900
Bsp1286I	GDGCHC	3 cut(s)	1006, 3349, 3588
Bsp1407I	TGTACA	2 cut(s)	2201, 2961
Bsp19I	CCATGG	1 cut(s)	1414
BspACI	CCGC	7 cut(s)	334, 779, 1248, 1448, 2560, 2692, 2998
BspANI	GGCC	9 cut(s)	509, 653, 815, 2050, 2318, 2357, 2989, 3087, 3755
BspCNI	CTCAG	9 cut(s)	502, 982, 2091, 2404, 2743, 2799, 3054, 3376, 3569
BspHI	TCATGA	4 cut(s)	1134, 2652, 2779, 3094
BspLI	GGNNCC	6 cut(s)	345, 1446, 1610, 1629, 2358, 3653
BspMI	ACCTGC	1 cut(s)	3494
BspPI	GGATC	8 cut(s)	1439, 1452, 1603, 1616, 2167, 2265, 2768, 2961
BspQI	GCTCTTC	1 cut(s)	1060
BspT104I	TTCGAA	1 cut(s)	900
BspT107I	GGYRCC	1 cut(s)	1627
BspTI	CTTAAG	2 cut(s)	2149, 3404
BspTNI	GGTCTC	3 cut(s)	833, 3583, 3645
BsrDI	GCAATG	3 cut(s)	695, 2605, 3603
BsrFI	RCCGGY	2 cut(s)	1352, 2195
BsrGI	TGTACA	2 cut(s)	2201, 2961
BsrI	ACTGG	3 cut(s)	381, 1609, 3315
BssAI	RCCGGY	2 cut(s)	1352, 2195
BssECI	CCNNGG	6 cut(s)	1414, 1785, 2045, 3054, 3349, 3750
BssSI	CACGAG	1 cut(s)	3138
BssT1I	CCWWGG	5 cut(s)	1414, 1785, 3054, 3349, 3750
Bst2BI	CACGAG	1 cut(s)	3138
Bst2UI	CCWGG	4 cut(s)	1580, 2046, 2255, 3520
Bst4CI	ACNGT	5 cut(s)	608, 2336, 2377, 3234, 3514
Bst6I	CTCTTC	4 cut(s)	86, 1060, 1496, 2464
BstAFI	CTTAAG	2 cut(s)	2149, 3404
BstAPI	GCANNNNNTGC	1 cut(s)	3598
BstAUI	TGTACA	2 cut(s)	2201, 2961
BstBI	TTCGAA	1 cut(s)	900
BstC8I	GCNNGC	8 cut(s)	1278, 1354, 1411, 2011, 2215, 2343, 3366, 3594
BstDSI	CCRYGG	1 cut(s)	1414
BstEII	GGTNACC	2 cut(s)	1120, 2040
BstF5I	GGATG	7 cut(s)	119, 856, 1769, 2042, 2250, 2374, 3183
BstHHI	GCGC	1 cut(s)	400
BstMAI	GTCTC	5 cut(s)	833, 1943, 3583, 3623, 3645
BstMWI	GCNNNNNNNGC	4 cut(s)	397, 688, 697, 3598
BstNI	CCWGG	4 cut(s)	1580, 2046, 2255, 3520
BstPI	GGTNACC	2 cut(s)	1120, 2040
BstSCI	CCNGG	5 cut(s)	1578, 2044, 2253, 2825, 3518
BstSFI	CTRYAG	1 cut(s)	2540
BstSLI	GKGCMC	2 cut(s)	1006, 3349
BstV1I	GCAGC	2 cut(s)	2125, 3702
BstV2I	GAAGAC	3 cut(s)	749, 1210, 3256
BstX2I	RGATCY	3 cut(s)	1444, 1608, 2927
BstYI	RGATCY	3 cut(s)	1444, 1608, 2927
BsuI	GTATCC	2 cut(s)	1564, 2556
BsuRI	GGCC	9 cut(s)	509, 653, 815, 2050, 2318, 2357, 2989, 3087, 3755
BtgI	CCRYGG	1 cut(s)	1414
BtsCI	GGATG	7 cut(s)	119, 856, 1769, 2042, 2250, 2374, 3183
BtsI	GCAGTG	5 cut(s)	94, 398, 707, 1956, 2115
BtsIMutI	CAGTG	8 cut(s)	94, 398, 613, 707, 1602, 1956, 2115, 3519
BveI	ACCTGC	1 cut(s)	3494
Cac8I	GCNNGC	8 cut(s)	1278, 1354, 1411, 2011, 2215, 2343, 3366, 3594
CciI	TCATGA	4 cut(s)	1134, 2652, 2779, 3094
CfoI	GCGC	1 cut(s)	400
Cfr10I	RCCGGY	2 cut(s)	1352, 2195
Cfr13I	GGNCC	4 cut(s)	651, 2048, 2356, 3436
Csp6I	GTAC	5 cut(s)	18, 1628, 2202, 2912, 2962
CspAI	ACCGGT	1 cut(s)	2195
CspCI	CAANNNNNGTGG	2 cut(s)	1887, 1922
CviQI	GTAC	5 cut(s)	18, 1628, 2202, 2912, 2962
DraI	TTTAAA	2 cut(s)	927, 2323
DrdI	GACNNNNNNGTC	2 cut(s)	3434, 3637
DriI	GACNNNNNGTC	1 cut(s)	2152
DseDI	GACNNNNNNGTC	2 cut(s)	3434, 3637
Eam1104I	CTCTTC	4 cut(s)	86, 1060, 1496, 2464
Eam1105I	GACNNNNNGTC	1 cut(s)	2152
EarI	CTCTTC	4 cut(s)	86, 1060, 1496, 2464
EciI	GGCGGA	1 cut(s)	794
Ecl136II	GAGCTC	1 cut(s)	3586
Eco130I	CCWWGG	5 cut(s)	1414, 1785, 3054, 3349, 3750
Eco24I	GRGCYC	1 cut(s)	3588
Eco31I	GGTCTC	3 cut(s)	833, 3583, 3645
Eco32I	GATATC	1 cut(s)	2136
Eco47I	GGWCC	1 cut(s)	3436
Eco53kI	GAGCTC	1 cut(s)	3586
Eco57I	CTGAAG	1 cut(s)	3308
Eco91I	GGTNACC	2 cut(s)	1120, 2040
EcoICRI	GAGCTC	1 cut(s)	3586
EcoO65I	GGTNACC	2 cut(s)	1120, 2040
EcoRII	CCWGG	4 cut(s)	1578, 2044, 2253, 3518
EcoRV	GATATC	1 cut(s)	2136
EcoT14I	CCWWGG	5 cut(s)	1414, 1785, 3054, 3349, 3750
EcoT22I	ATGCAT	2 cut(s)	1038, 2740
EcoT38I	GRGCYC	1 cut(s)	3588
ErhI	CCWWGG	5 cut(s)	1414, 1785, 3054, 3349, 3750
FalI	AAGNNNNNCTT	8 cut(s)	270, 302, 348, 380, 947, 979, 1805, 1837
FaqI	GGGAC	4 cut(s)	1072, 1327, 1731, 3440
FauI	CCCGC	2 cut(s)	327, 1241
FauNDI	CATATG	1 cut(s)	2169
FbaI	TGATCA	1 cut(s)	721
FblI	GTMKAC	1 cut(s)	1331
Fnu4HI	GCNGC	2 cut(s)	2114, 3691
FokI	GGATG	7 cut(s)	106, 863, 1756, 2049, 2237, 2381, 3190
FriOI	GRGCYC	1 cut(s)	3588
Fsp4HI	GCNGC	2 cut(s)	2114, 3691
FspBI	CTAG	7 cut(s)	417, 912, 1613, 1835, 2477, 3666, 3735
FspI	TGCGCA	1 cut(s)	399
GlaI	GCGC	1 cut(s)	399
GluI	GCNGC	2 cut(s)	2114, 3691
GsaI	CCCAGC	2 cut(s)	1023, 1284
GsuI	CTGGAG	2 cut(s)	1165, 3380
HaeIII	GGCC	9 cut(s)	509, 653, 815, 2050, 2318, 2357, 2989, 3087, 3755
HapII	CCGG	5 cut(s)	1353, 1711, 2051, 2196, 2826
HhaI	GCGC	1 cut(s)	400
Hin6I	GCGC	1 cut(s)	398
HinP1I	GCGC	1 cut(s)	398
HincII	GTYRAC	3 cut(s)	1332, 2428, 3211
HindII	GTYRAC	3 cut(s)	1332, 2428, 3211
HindIII	AAGCTT	3 cut(s)	1814, 2183, 3710
HpaI	GTTAAC	1 cut(s)	2428
HpaII	CCGG	5 cut(s)	1353, 1711, 2051, 2196, 2826
HphI	GGTGA	6 cut(s)	18, 76, 650, 1786, 1921, 3518
Hpy166II	GTNNAC	9 cut(s)	18, 26, 1157, 1183, 1332, 2428, 2455, 2964, 3211
Hpy8I	GTNNAC	9 cut(s)	18, 26, 1157, 1183, 1332, 2428, 2455, 2964, 3211
HpyAV	CCTTC	7 cut(s)	293, 805, 880, 952, 1279, 1672, 2714
HpyCH4III	ACNGT	5 cut(s)	608, 2336, 2377, 3234, 3514
HpyF10VI	GCNNNNNNNGC	4 cut(s)	397, 688, 697, 3598
HspAI	GCGC	1 cut(s)	398
KpnI	GGTACC	1 cut(s)	1631
KroI	GCCGGC	1 cut(s)	1352
KroNI	GCCGGC	1 cut(s)	1354
Ksp22I	TGATCA	1 cut(s)	721
KspAI	GTTAAC	1 cut(s)	2428
LguI	GCTCTTC	1 cut(s)	1060
LmnI	GCTCC	7 cut(s)	343, 1146, 1999, 2638, 2857, 3361, 3583
Lsp1109I	GCAGC	2 cut(s)	2125, 3702
LweI	GCATC	6 cut(s)	1778, 1939, 2259, 2563, 2799, 3423
MaeI	CTAG	7 cut(s)	417, 912, 1613, 1835, 2477, 3666, 3735
MfeI	CAATTG	1 cut(s)	725
MflI	RGATCY	3 cut(s)	1444, 1608, 2927
MhlI	GDGCHC	3 cut(s)	1006, 3349, 3588
MlyI	GAGTC	3 cut(s)	437, 1608, 3073
MmeI	TCCRAC	4 cut(s)	385, 567, 1770, 3372
Mph1103I	ATGCAT	2 cut(s)	1038, 2740
MroNI	GCCGGC	1 cut(s)	1352
MroXI	GAANNNNTTC	2 cut(s)	2127, 3165
MslI	CAYNNNNRTG	1 cut(s)	700
MspA1I	CMGCKG	1 cut(s)	1248
MspCI	CTTAAG	2 cut(s)	2149, 3404
MspI	CCGG	5 cut(s)	1353, 1711, 2051, 2196, 2826
MspR9I	CCNGG	5 cut(s)	1580, 2046, 2255, 2827, 3520
MunI	CAATTG	1 cut(s)	725
Mva1269I	GAATGC	4 cut(s)	1036, 1664, 2011, 2127
MvaI	CCWGG	4 cut(s)	1580, 2046, 2255, 3520
MwoI	GCNNNNNNNGC	4 cut(s)	397, 688, 697, 3598
NaeI	GCCGGC	1 cut(s)	1354
NciI	CCSGG	1 cut(s)	2827
NcoI	CCATGG	1 cut(s)	1414
NdeI	CATATG	1 cut(s)	2169
NgoMIV	GCCGGC	1 cut(s)	1352
NlaIV	GGNNCC	6 cut(s)	345, 1446, 1610, 1629, 2358, 3653
NmuCI	GTSAC	4 cut(s)	1492, 1600, 3514, 3668
NsbI	TGCGCA	1 cut(s)	399
NsiI	ATGCAT	2 cut(s)	1038, 2740
NspV	TTCGAA	1 cut(s)	900
PagI	TCATGA	4 cut(s)	1134, 2652, 2779, 3094
PaqCI	CACCTGC	1 cut(s)	3494
PciSI	GCTCTTC	1 cut(s)	1060
PctI	GAATGC	4 cut(s)	1036, 1664, 2011, 2127
PdiI	GCCGGC	1 cut(s)	1354
PdmI	GAANNNNTTC	2 cut(s)	2127, 3165
PfoI	TCCNGGA	1 cut(s)	2253
PinAI	ACCGGT	1 cut(s)	2195
PkrI	GCNGC	2 cut(s)	2115, 3692
PleI	GAGTC	3 cut(s)	436, 1607, 3072
PpsI	GAGTC	3 cut(s)	436, 1607, 3072
PshBI	ATTAAT	2 cut(s)	2384, 3543
PsiI	TTATAA	2 cut(s)	2271, 3017
Psp124BI	GAGCTC	1 cut(s)	3588
Psp6I	CCWGG	4 cut(s)	1578, 2044, 2253, 3518
PspEI	GGTNACC	2 cut(s)	1120, 2040
PspFI	CCCAGC	2 cut(s)	1019, 1280
PspGI	CCWGG	4 cut(s)	1578, 2044, 2253, 3518
PspN4I	GGNNCC	6 cut(s)	345, 1446, 1610, 1629, 2358, 3653
PspPI	GGNCC	4 cut(s)	651, 2048, 2356, 3436
PsuI	RGATCY	3 cut(s)	1444, 1608, 2927
RsaI	GTAC	5 cut(s)	19, 1629, 2203, 2913, 2963
RsaNI	GTAC	5 cut(s)	18, 1628, 2202, 2912, 2962
RseI	CAYNNNNRTG	1 cut(s)	700
SacI	GAGCTC	1 cut(s)	3588
SalI	GTCGAC	1 cut(s)	1330
SapI	GCTCTTC	1 cut(s)	1060
SatI	GCNGC	2 cut(s)	2114, 3691
Sau96I	GGNCC	4 cut(s)	651, 2048, 2356, 3436
SchI	GAGTC	3 cut(s)	437, 1608, 3073
ScrFI	CCNGG	5 cut(s)	1580, 2046, 2255, 2827, 3520
SduI	GDGCHC	3 cut(s)	1006, 3349, 3588
SfaNI	GCATC	6 cut(s)	1778, 1939, 2259, 2563, 2799, 3423
SfcI	CTRYAG	1 cut(s)	2540
SfuI	TTCGAA	1 cut(s)	900
SinI	GGWCC	1 cut(s)	3436
SmiMI	CAYNNNNRTG	1 cut(s)	700
SmlI	CTYRAG	5 cut(s)	905, 1993, 2149, 3004, 3404
SmoI	CTYRAG	5 cut(s)	905, 1993, 2149, 3004, 3404
SsiI	CCGC	7 cut(s)	334, 779, 1248, 1448, 2560, 2692, 2998
SspI	AATATT	5 cut(s)	233, 2020, 2278, 2800, 3746
SspMI	CTAG	7 cut(s)	417, 912, 1613, 1835, 2477, 3666, 3735
SstI	GAGCTC	1 cut(s)	3588
StyD4I	CCNGG	5 cut(s)	1578, 2044, 2253, 2825, 3518
StyI	CCWWGG	5 cut(s)	1414, 1785, 3054, 3349, 3750
TaaI	ACNGT	5 cut(s)	608, 2336, 2377, 3234, 3514
TaqI	TCGA	8 cut(s)	670, 760, 900, 1331, 2942, 3067, 3417, 3627
TatI	WGTACW	3 cut(s)	2201, 2911, 2961
TscAI	CASTG	8 cut(s)	101, 398, 613, 707, 1609, 1963, 2115, 3519
TseFI	GTSAC	4 cut(s)	1492, 1600, 3514, 3668
TseI	GCWGC	2 cut(s)	2113, 3690
Tsp45I	GTSAC	4 cut(s)	1492, 1600, 3514, 3668
TspDTI	ATGAA	9 cut(s)	108, 945, 1395, 1716, 2300, 2723, 2904, 3083, 3368
TspGWI	ACGGA	1 cut(s)	2049
TspRI	CASTG	8 cut(s)	101, 398, 613, 707, 1609, 1963, 2115, 3519
Vha464I	CTTAAG	2 cut(s)	2149, 3404
VpaK11BI	GGWCC	1 cut(s)	3436
VspI	ATTAAT	2 cut(s)	2384, 3543
XapI	RAATTY	9 cut(s)	441, 600, 874, 1095, 1372, 1672, 2305, 2350, 2580
XbaI	TCTAGA	1 cut(s)	2476
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1462
XmiI	GTMKAC	1 cut(s)	1331
XmnI	GAANNNNTTC	2 cut(s)	2127, 3165
XspI	CTAG	7 cut(s)	417, 912, 1613, 1835, 2477, 3666, 3735
Zsp2I	ATGCAT	2 cut(s)	1038, 2740

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.