Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0002362

Physical Location & Seq

Genomic Coordinates Reverse (-)

77359597 .. 77363401

Gene Structure 3805 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G049750.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3735 bp

ATGAGACAAAGCAGCACCACCATAGCTCTATGCAATGAGATGCTTCAAGTTTTGTGTACCTGTTCACCCCACAAGCCTCACATCAAACAGGTTGGTTCTCTTTACTCTCTGTTCTCACTCTTCACCAATAACCACTGCCTCTCAACTACCACTTCTTCATCCCCAATTCCCCATCATCTTCAACAACACACAAAAACCAACACCCAAGTAGATTTATCTCCTAATTGTTTCAATGGTATAGCCCAATCTGTTATATTGAGGTGTTCCCAATATTTTGACAAGAGTAAGGGCAAAGACTTTGCCAATGCTTCTCTAAAAGACCTTCTTTTGGAGATTTCTGATTTAGTGCCTGAACAAACTCGTAGGCTTAGGCGGGTTTCGGCTCCGAAACCCGAAGATGTGCTTGAATTATTACTGGGTTTTGAATTGCAGTGTGCGCAAGTTGGATTTGAAGCTAGAAAGGTTGAGTCTTTGTGGGGAATTTTCAAGTGGGTTAGTGAAAAAGTTGAGGGCTTTAAGCATAAACCTCAGTCATGTGAGGTCATGGCCTCGTTGCTTCTCCGAGTGGGATTGCTTAGAGAAGTTGAGTTCTTGCTTTCCACAATGGAAAGTCAAGGAGTTTGGTATGTTGGTGTTGGTGAGTTAGATAGGGCCATTTCTGTCTATGATCGAATTACGGGGCGTGTAGTGCCATCATTGCAGTGTTGTTGTGTTCTACTTGATCAATTGGTGGGAATGAGGAAGACCCAATTGGCGTTTCGAGTTTGTAGTGATATGGTGGAAATGGGTTTTGATTTGAGAGATGTGAAGAAGGCCACATTTGAGGGTCTCATTAGACTGCTTTGCAGGGATGGAAAGATTCAGGACGCAAGAAATTTTGTCAAGAAGGCTATGGCTTTCGAACTCAAGCCTAGCAACTTAGTTTTAAATGAAGTTGCTTATGGATATTGTGAGAAGAAGGACTTTGATGATTTGATGAGCTTCTATTCTGAGATTAAGTGTGCCCCAGAAGTTATGGCTGGGAATAGGATTATGCATTCTCTTTGTAGCCATTTTGGGACAAGAAGAGCAGAACTTTATTTGCAAGAATTAGAAATTTTAGGCTTCAATCCTGATGAGGTAACCTTTGGAATCATGATTGGCTGGAGCTGTCGTGAACGAAAACTTAAAAGTGCCTTTGTTTACCTATCAGAGATGTTGGGAAGACACCTAAACCCCCATATATGTACCTATAATGCTCTTATCAGCGGGGTATTTATGGAGGGTATGTGGAAGCACGCTGGGAAGGTGTTTGATGAGATGGTAGATAGGGGGACTACACCTGATTTGTCGACTTTCAGGATTCTCTTAGCAGGCTATTGTAAAGTCAGAAAATTTGATGAAGCCAAAAGGATAGTTTTTGATATGGCAAGCCATGGACTGATTCAAAACTCTTCAGATGAGGATCCGCTATCCAAAGCATTTATGGTTCTGGGTTTCAAGCCATTAGCTGTGACATTGAAGAGAGATAATGATGTTGGGTTTGCTAAAACAGAGTTTTATGATGATCTTGGGAATGGACTTTATTTGGATACAGACCTGGATAAGTATGAGAAAAGAGTCACTGGGATCCTAGAAGATTGCATGGTACCTGATTATTACTCTCTTATGATGATAGAATGCAGTCATGAAATGATCCGGTGGGGACAAGATTTGTCATTGTCTATGATGTCCGACTTACTGAAAGGGCTTTCTGCATCCCATTTACATACCAAGGGTATCACCAGCATTGTAGATAAAAAGCTTCACTTGGTAAATCAGCTAGACCAAGAAACTCTAAACTTTCTTGCCCAAGCATACGGCAAGAAAGGATTGACATATAATACAAGGATAGTGGTAAATGGAATGATAGAAAGGCATCTGAAAATCAACAATGAGACTTACACTGCTTTAGTAAAAGGTTTTTGTAAGAAAGGAAACTTGAGGGAGCTGAATGCTTGCTGGAATATTGCTCAAAATGACGGATGGTTACCAGGGCCGGAAGATTGTAAAGCCTTGATTGAATGTCTTTTCCATAAAGAAATGCTAAGAGAAGCAGTGCAGCTTCTTGAAAGCATTCTGATATCCTACCCAGACTTAAGGTCAGATATGTGTCATATGATCCTTGACAAGCTTTTTGTTACCGGTTTTACAGGAATTGCAAGCATATTGTTAGAAGAACTTGAACAGCGTGGTGGCATCCTGGATCAGATGGCTTATAACAATATTATTAGAGGATTGTGTAAGGGCAAGAATTTTCATGTGGCCTTTACAGTGCTGGACAGTATGCTGGCTAAAAATTTGGCCCCTTGTTTGGATGTTACTGTCCAATTAATTCCTCAGTTGTGTAGGGCTGATAGATTTGGAAAAGCAGTTAACTTGAAAGAGATTGGTCTAAGAGAGAACTCTTCCTATTCACTTTCTCTAGATCGTGCATTGATAAAAGGCTGTTGTATTTCAGGGAAAGTTACAGAAGCAACCACTCTACTACAGAATATGTTGTTAAAGCGGATACCTCCTGATGCTGAAATTTACAATTTTCTGGTTCAGGGTCATTGCAAGGTTAATGACTTAAAGAAAGTTTGGGAGCTACTTTGTGTCATGACAAGAAAAGGTTTCAACATTTCACTCTCAACTTACCGCAACTTGGTGTGTTTGATGTGTCTGGAAGGTAGGGTTCTTCATGCATGGAAATTGACTGAGCTTATGATTGGACAAAGTGATCCTCATGATCTCAGCATCTACAATATTCTGATATTCTATATTTTCCCAACCGGGAATACTTTGCTTGTCAAGAAAGTTGTGGAGCATTTGCAAGAGAAGAAATTGCTACTTGATGAAGTAACTTACAACTTTCTTGTACTTGGGTTTTGTAGATCTAAAGATGTGTCGAGTGCTGTGGATCATCTGTACACTATGATCTCTAAGGATTTCAGGCCAAGCAACCGCAACTTGAGAAAAGTTATAACCAGCCTGTGTGATGTTGGGGAGATTGAGAAAGCCTTGGAACTGAGTCAAGAAATGGAATTGAGAGGCCAGATTCATGATTCAATGATTCAAAATGCGATTGTTGAGGGTCTTTCTTCTCGTGGTAGGATTCAAGAAGCTGAAAGTTTTCTGGACAGGATGGTAGAGAAATGTCTAATTCCAGAAAATGTCAACTATAATAATCTAATCAAACTGTTTTGCTCGTATGGAAGACCAAACAAGGCAGTTAATTCTCTGAACATAATGCTGAAGAAAGGGAATGTTCCAGATTCTACCAGTTTTGATTCCATCATCAGCTCTTTCTGTGCCCTTGGTAATCTGGAGCAAGCTATGGATTTTCATGCTGAGATGTTGGATAGAAATCTTAAGCCAAGCATCGACACTTGGGACATTCTGGTCCATAACTTTTGTCAAGATGGAAAAACATCAGAGGCAGAAAGGCTATTGAAGTCTATGGGTTGTGCAGGTGAAACAGTGACCAGGAAGATATATCTGTCTGTAATTAATAGTTATCGCTCAGAGAACAATCTCGGAAAGGTCTCGGAGCTCATGCAAGCAATGCAACAGAGTGGTTATGAGCCAGACTTCGAGACACATTGGTCTCTCATAAGGAACCTAAGAGTTTCTAGTGACAAGGACAATGCAAACAGCAGCAAGGGTTTCCTGTCAAAGCTTCTCTCTGCAAGTGGATTTTCTAGGCAAAAGTATTCCAAGGCCAAACGGGGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0048037 GO:0051536 GO:0051540 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2

Protein Analysis

1244

Amino Acids

140.81

Weight (kDa)

7.58

Isoelectric Point (pI)

34.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_3	PF13812	361 - 416	6.8e-06	Pentatricopeptide repeat domain
PPR_2	PF13041	406 - 455	4.4e-14	PPR repeat family
PPR_2	PF13041	846 - 894	1.8e-07	PPR repeat family
TPR_24	PF23276	932 - 1043	1.6e-08	Fungal tetratrico peptide repeats
PPR_2	PF13041	1057 - 1104	4.6e-09	PPR repeat family
PPR_2	PF13041	1091 - 1140	2.6e-09	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002362)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G15280
fragaria_vesca	FvH4_6g47953 FvH4_6g48150 FvH4_6g48150 FvH4_6g48150 FvH4_6g48150 FvH4_6g48150 FvH4_6g48880 FvH4_6g48880
malus_domestica	MD09G1053400.v1.1
prunus_persica	Prupe.3G265200_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0167491 RchiOBHm_Chr2g0167571 RchiOBHm_Chr2g0167581
rosa_laevigata	RLG00000021724 RLG00000021736
rosa_multiflora	Rmu_sc0000056.1_g000010 Rmu_sc0000056.1_g000011 Rmu_sc0001970.1_g000002
rosa_roxburghii	Rroxscaffold_2G00083370 Rroxscaffold_2G00083410 Rroxscaffold_2G00083440
rosa_rugosa	Rorug02G0530600
rosa_samantha	Rh2AG597900 Rh2AG598800 Rh2AG599200 Rh2AG599300 Rh2AG599400 Rh2BG609400 Rh2BG609800 Rh2BG609900 Rh2BG610000 Rh2CG580300 Rh2DG620700 Rh2DG621000 Rh2DG621100 Rh2DG621900 Rh2DG622000 Rh2DG622100 Rh2DG622600 Rh2DG622700
rosa_wichuraiana	Rw2G049750

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	2238, 2984
AarI	CACCTGC	1 cut(s)	3461
AasI	GACNNNNNNGTC	2 cut(s)	3401, 3604
Acc16I	TGCGCA	1 cut(s)	438
Acc36I	ACCTGC	1 cut(s)	3461
Acc65I	GGTACC	1 cut(s)	1627
AccB1I	GGYRCC	1 cut(s)	1627
AccI	GTMKAC	1 cut(s)	1331
AciI	CCGC	6 cut(s)	373, 1248, 1448, 2527, 2659, 2965
AclWI	GGATC	9 cut(s)	1439, 1452, 1603, 1616, 1669, 2134, 2232, 2735, 2928
AcsI	RAATTY	7 cut(s)	480, 874, 1095, 1373, 2272, 2317, 2547
AcuI	CTGAAG	2 cut(s)	1419, 3275
AfaI	GTAC	5 cut(s)	58, 1228, 1629, 2880, 2930
AfiI	CCNNNNNNNGG	4 cut(s)	328, 2332, 2665, 3729
AflII	CTTAAG	2 cut(s)	2116, 3371
AgeI	ACCGGT	1 cut(s)	2162
AhdI	GACNNNNNGTC	1 cut(s)	2119
AjnI	CCWGG	4 cut(s)	1578, 2011, 2220, 3485
AjuI	GAANNNNNNNTTGG	4 cut(s)	734, 741, 766, 773
Alw21I	GWGCWC	1 cut(s)	3555
Alw26I	GTCTC	5 cut(s)	833, 1910, 3550, 3590, 3612
AlwI	GGATC	9 cut(s)	1439, 1452, 1603, 1616, 1669, 2134, 2232, 2735, 2928
AoxI	GGCC	9 cut(s)	546, 651, 813, 2015, 2283, 2322, 2954, 3052, 3720
ApeKI	GCWGC	3 cut(s)	12, 2080, 3657
ApoI	RAATTY	7 cut(s)	480, 874, 1095, 1373, 2272, 2317, 2547
ArsI	GACNNNNNNTTYG	2 cut(s)	2138, 2170
AseI	ATTAAT	2 cut(s)	2351, 3510
AsiGI	ACCGGT	1 cut(s)	2162
Asp700I	GAANNNNTTC	2 cut(s)	2094, 3132
Asp718I	GGTACC	1 cut(s)	1627
AspLEI	GCGC	1 cut(s)	439
AspS9I	GGNCC	4 cut(s)	651, 2015, 2323, 3403
AsuC2I	CCSGG	1 cut(s)	2794
AsuHPI	GGTGA	5 cut(s)	57, 115, 650, 1753, 3485
AsuII	TTCGAA	1 cut(s)	900
AvaII	GGWCC	1 cut(s)	3403
BaeGI	GKGCMC	2 cut(s)	1006, 3316
BamHI	GGATCC	2 cut(s)	1444, 1608
BanI	GGYRCC	1 cut(s)	1627
BanII	GRGCYC	1 cut(s)	3555
BauI	CACGAG	1 cut(s)	3105
BbsI	GAAGAC	3 cut(s)	749, 1210, 3223
Bbv12I	GWGCWC	1 cut(s)	3555
BbvI	GCAGC	3 cut(s)	24, 2092, 3669
BccI	CCATC	9 cut(s)	180, 700, 845, 1294, 1998, 2224, 3139, 3302, 3416
BceAI	ACGGC	1 cut(s)	1855
BciT130I	CCWGG	4 cut(s)	1580, 2013, 2222, 3487
BciVI	GTATCC	2 cut(s)	1564, 2523
BclI	TGATCA	1 cut(s)	721
BcnI	CCSGG	1 cut(s)	2794
BcoDI	GTCTC	5 cut(s)	833, 1910, 3550, 3590, 3612
BfaI	CTAG	7 cut(s)	456, 912, 1613, 1802, 2444, 3633, 3702
BfmI	CTRYAG	1 cut(s)	2507
BfrI	CTTAAG	2 cut(s)	2116, 3371
BfuAI	ACCTGC	1 cut(s)	3461
BfuI	GTATCC	2 cut(s)	1564, 2523
BglII	AGATCT	1 cut(s)	2894
BisI	GCNGC	3 cut(s)	13, 2081, 3658
BlsI	GCNGC	3 cut(s)	14, 2082, 3659
Bme1390I	CCNGG	5 cut(s)	1580, 2013, 2222, 2794, 3487
Bme18I	GGWCC	1 cut(s)	3403
BmeRI	GACNNNNNGTC	1 cut(s)	2119
BmgT120I	GGNCC	4 cut(s)	651, 2015, 2323, 3403
BmiI	GGNNCC	6 cut(s)	384, 1446, 1610, 1629, 2325, 3620
BmrFI	CCNGG	5 cut(s)	1580, 2013, 2222, 2794, 3487
BmrI	ACTGGG	2 cut(s)	425, 1614
BmsI	GCATC	7 cut(s)	30, 1745, 1906, 2226, 2530, 2766, 3390
BmuI	ACTGGG	2 cut(s)	425, 1614
BpiI	GAAGAC	3 cut(s)	749, 1210, 3223
BpmI	CTGGAG	2 cut(s)	1165, 3347
Bpu10I	CCTNAGC	1 cut(s)	368
Bpu14I	TTCGAA	1 cut(s)	900
BpuEI	CTTGAG	3 cut(s)	890, 1981, 2992
BpuMI	CCSGG	1 cut(s)	2794
BsaBI	GATNNNNATC	2 cut(s)	1443, 3366
BsaI	GGTCTC	3 cut(s)	833, 3550, 3612
BsaJI	CCNNGG	6 cut(s)	1414, 1752, 2012, 3021, 3316, 3717
BsaWI	WCCGGW	2 cut(s)	1677, 2162
BsaXI	ACNNNNNCTCC	4 cut(s)	1138, 1168, 1253, 1283
Bsc4I	CCNNNNNNNGG	4 cut(s)	328, 2332, 2665, 3729
Bse118I	RCCGGY	1 cut(s)	2162
Bse1I	ACTGG	3 cut(s)	420, 1609, 3282
Bse3DI	GCAATG	4 cut(s)	40, 695, 2572, 3570
Bse8I	GATNNNNATC	2 cut(s)	1443, 3366
BseBI	CCWGG	4 cut(s)	1580, 2013, 2222, 3487
BseDI	CCNNGG	6 cut(s)	1414, 1752, 2012, 3021, 3316, 3717
BseGI	GGATG	7 cut(s)	158, 856, 1736, 2009, 2217, 2341, 3150
BseJI	GATNNNNATC	2 cut(s)	1443, 3366
BseLI	CCNNNNNNNGG	4 cut(s)	328, 2332, 2665, 3729
BseMI	GCAATG	4 cut(s)	40, 695, 2572, 3570
BseMII	CTCAG	8 cut(s)	542, 981, 2372, 2709, 2767, 3020, 3342, 3537
BseNI	ACTGG	3 cut(s)	420, 1609, 3282
BseSI	GKGCMC	2 cut(s)	1006, 3316
BseXI	GCAGC	3 cut(s)	24, 2092, 3669
BseYI	CCCAGC	2 cut(s)	1019, 1280
BsgI	GTGCAG	2 cut(s)	2099, 3489
BshFI	GGCC	9 cut(s)	548, 653, 815, 2017, 2285, 2324, 2956, 3054, 3722
BshNI	GGYRCC	1 cut(s)	1627
BshTI	ACCGGT	1 cut(s)	2162
BsiHKAI	GWGCWC	1 cut(s)	3555
BsiSI	CCGG	4 cut(s)	1678, 2018, 2163, 2793
BslFI	GGGAC	4 cut(s)	1072, 1327, 1698, 3407
BslI	CCNNNNNNNGG	4 cut(s)	328, 2332, 2665, 3729
BsmAI	GTCTC	5 cut(s)	833, 1910, 3550, 3590, 3612
BsmFI	GGGAC	4 cut(s)	1072, 1327, 1698, 3407
BsmI	GAATGC	4 cut(s)	1036, 1664, 1978, 2094
BsnI	GGCC	9 cut(s)	548, 653, 815, 2017, 2285, 2324, 2956, 3054, 3722
Bso31I	GGTCTC	3 cut(s)	833, 3550, 3612
Bsp119I	TTCGAA	1 cut(s)	900
Bsp1286I	GDGCHC	3 cut(s)	1006, 3316, 3555
Bsp1407I	TGTACA	1 cut(s)	2928
Bsp19I	CCATGG	1 cut(s)	1414
BspACI	CCGC	6 cut(s)	373, 1248, 1448, 2527, 2659, 2965
BspANI	GGCC	9 cut(s)	548, 653, 815, 2017, 2285, 2324, 2956, 3054, 3722
BspCNI	CTCAG	8 cut(s)	541, 982, 2371, 2710, 2766, 3021, 3343, 3536
BspHI	TCATGA	5 cut(s)	1134, 1666, 2619, 2746, 3061
BspLI	GGNNCC	6 cut(s)	384, 1446, 1610, 1629, 2325, 3620
BspMI	ACCTGC	1 cut(s)	3461
BspPI	GGATC	9 cut(s)	1439, 1452, 1603, 1616, 1669, 2134, 2232, 2735, 2928
BspQI	GCTCTTC	1 cut(s)	1060
BspT104I	TTCGAA	1 cut(s)	900
BspT107I	GGYRCC	1 cut(s)	1627
BspTI	CTTAAG	2 cut(s)	2116, 3371
BspTNI	GGTCTC	3 cut(s)	833, 3550, 3612
BsrDI	GCAATG	4 cut(s)	40, 695, 2572, 3570
BsrFI	RCCGGY	1 cut(s)	2162
BsrGI	TGTACA	1 cut(s)	2928
BsrI	ACTGG	3 cut(s)	420, 1609, 3282
BssAI	RCCGGY	1 cut(s)	2162
BssECI	CCNNGG	6 cut(s)	1414, 1752, 2012, 3021, 3316, 3717
BssSI	CACGAG	1 cut(s)	3105
BssT1I	CCWWGG	5 cut(s)	1414, 1752, 3021, 3316, 3717
Bst2BI	CACGAG	1 cut(s)	3105
Bst2UI	CCWGG	4 cut(s)	1580, 2013, 2222, 3487
Bst4CI	ACNGT	5 cut(s)	2293, 2303, 2344, 3201, 3481
Bst6I	CTCTTC	5 cut(s)	125, 1060, 1438, 1496, 2431
BstAFI	CTTAAG	2 cut(s)	2116, 3371
BstAPI	GCANNNNNTGC	1 cut(s)	3565
BstAUI	TGTACA	1 cut(s)	2928
BstBI	TTCGAA	1 cut(s)	900
BstC8I	GCNNGC	8 cut(s)	1278, 1354, 1411, 1978, 2182, 2310, 3333, 3561
BstDSI	CCRYGG	1 cut(s)	1414
BstEII	GGTNACC	2 cut(s)	1120, 2007
BstF5I	GGATG	7 cut(s)	158, 856, 1736, 2009, 2217, 2341, 3150
BstHHI	GCGC	1 cut(s)	439
BstMAI	GTCTC	5 cut(s)	833, 1910, 3550, 3590, 3612
BstMWI	GCNNNNNNNGC	4 cut(s)	436, 688, 697, 3565
BstNI	CCWGG	4 cut(s)	1580, 2013, 2222, 3487
BstPI	GGTNACC	2 cut(s)	1120, 2007
BstSCI	CCNGG	5 cut(s)	1578, 2011, 2220, 2792, 3485
BstSFI	CTRYAG	1 cut(s)	2507
BstSLI	GKGCMC	2 cut(s)	1006, 3316
BstV1I	GCAGC	3 cut(s)	24, 2092, 3669
BstV2I	GAAGAC	3 cut(s)	749, 1210, 3223
BstX2I	RGATCY	3 cut(s)	1444, 1608, 2894
BstYI	RGATCY	3 cut(s)	1444, 1608, 2894
BsuI	GTATCC	2 cut(s)	1564, 2523
BsuRI	GGCC	9 cut(s)	548, 653, 815, 2017, 2285, 2324, 2956, 3054, 3722
BtgI	CCRYGG	1 cut(s)	1414
BtsCI	GGATG	7 cut(s)	158, 856, 1736, 2009, 2217, 2341, 3150
BtsI	GCAGTG	5 cut(s)	133, 437, 707, 1923, 2082
BtsIMutI	CAGTG	8 cut(s)	133, 437, 707, 1602, 1923, 2082, 2298, 3486
BveI	ACCTGC	1 cut(s)	3461
Cac8I	GCNNGC	8 cut(s)	1278, 1354, 1411, 1978, 2182, 2310, 3333, 3561
CciI	TCATGA	5 cut(s)	1134, 1666, 2619, 2746, 3061
CfoI	GCGC	1 cut(s)	439
Cfr10I	RCCGGY	1 cut(s)	2162
Cfr13I	GGNCC	4 cut(s)	651, 2015, 2323, 3403
CseI	GACGC	1 cut(s)	875
Csp6I	GTAC	5 cut(s)	57, 1227, 1628, 2879, 2929
CspAI	ACCGGT	1 cut(s)	2162
CspCI	CAANNNNNGTGG	2 cut(s)	1854, 1889
CviQI	GTAC	5 cut(s)	57, 1227, 1628, 2879, 2929
DraI	TTTAAA	1 cut(s)	927
DrdI	GACNNNNNNGTC	2 cut(s)	3401, 3604
DriI	GACNNNNNGTC	1 cut(s)	2119
DseDI	GACNNNNNNGTC	2 cut(s)	3401, 3604
Eam1104I	CTCTTC	5 cut(s)	125, 1060, 1438, 1496, 2431
Eam1105I	GACNNNNNGTC	1 cut(s)	2119
EarI	CTCTTC	5 cut(s)	125, 1060, 1438, 1496, 2431
Ecl136II	GAGCTC	1 cut(s)	3553
Eco130I	CCWWGG	5 cut(s)	1414, 1752, 3021, 3316, 3717
Eco24I	GRGCYC	1 cut(s)	3555
Eco31I	GGTCTC	3 cut(s)	833, 3550, 3612
Eco32I	GATATC	1 cut(s)	2103
Eco47I	GGWCC	1 cut(s)	3403
Eco53kI	GAGCTC	1 cut(s)	3553
Eco57I	CTGAAG	2 cut(s)	1419, 3275
Eco91I	GGTNACC	2 cut(s)	1120, 2007
EcoICRI	GAGCTC	1 cut(s)	3553
EcoO65I	GGTNACC	2 cut(s)	1120, 2007
EcoRII	CCWGG	4 cut(s)	1578, 2011, 2220, 3485
EcoRV	GATATC	1 cut(s)	2103
EcoT14I	CCWWGG	5 cut(s)	1414, 1752, 3021, 3316, 3717
EcoT22I	ATGCAT	2 cut(s)	1038, 2707
EcoT38I	GRGCYC	1 cut(s)	3555
ErhI	CCWWGG	5 cut(s)	1414, 1752, 3021, 3316, 3717
FalI	AAGNNNNNCTT	8 cut(s)	309, 341, 387, 419, 947, 979, 1772, 1804
FaqI	GGGAC	4 cut(s)	1072, 1327, 1698, 3407
FauI	CCCGC	2 cut(s)	366, 1241
FauNDI	CATATG	1 cut(s)	2136
FbaI	TGATCA	1 cut(s)	721
FblI	GTMKAC	1 cut(s)	1331
Fnu4HI	GCNGC	3 cut(s)	13, 2081, 3658
FokI	GGATG	7 cut(s)	145, 863, 1723, 2016, 2204, 2348, 3157
FriOI	GRGCYC	1 cut(s)	3555
Fsp4HI	GCNGC	3 cut(s)	13, 2081, 3658
FspBI	CTAG	7 cut(s)	456, 912, 1613, 1802, 2444, 3633, 3702
FspI	TGCGCA	1 cut(s)	438
GlaI	GCGC	1 cut(s)	438
GluI	GCNGC	3 cut(s)	13, 2081, 3658
GsaI	CCCAGC	2 cut(s)	1023, 1284
GsuI	CTGGAG	2 cut(s)	1165, 3347
HaeIII	GGCC	9 cut(s)	548, 653, 815, 2017, 2285, 2324, 2956, 3054, 3722
HapII	CCGG	4 cut(s)	1678, 2018, 2163, 2793
HgaI	GACGC	1 cut(s)	875
HhaI	GCGC	1 cut(s)	439
Hin6I	GCGC	1 cut(s)	437
HinP1I	GCGC	1 cut(s)	437
HincII	GTYRAC	3 cut(s)	1332, 2395, 3178
HindII	GTYRAC	3 cut(s)	1332, 2395, 3178
HindIII	AAGCTT	3 cut(s)	1781, 2150, 3677
HpaI	GTTAAC	1 cut(s)	2395
HpaII	CCGG	4 cut(s)	1678, 2018, 2163, 2793
HphI	GGTGA	5 cut(s)	57, 115, 650, 1753, 3485
Hpy166II	GTNNAC	8 cut(s)	57, 65, 1157, 1183, 1332, 2395, 2931, 3178
Hpy8I	GTNNAC	8 cut(s)	57, 65, 1157, 1183, 1332, 2395, 2931, 3178
HpyAV	CCTTC	6 cut(s)	332, 805, 880, 952, 1279, 2681
HpyCH4III	ACNGT	5 cut(s)	2293, 2303, 2344, 3201, 3481
HpyF10VI	GCNNNNNNNGC	4 cut(s)	436, 688, 697, 3565
HspAI	GCGC	1 cut(s)	437
KpnI	GGTACC	1 cut(s)	1631
Ksp22I	TGATCA	1 cut(s)	721
KspAI	GTTAAC	1 cut(s)	2395
LguI	GCTCTTC	1 cut(s)	1060
LmnI	GCTCC	7 cut(s)	388, 1146, 1966, 2605, 2824, 3328, 3550
Lsp1109I	GCAGC	3 cut(s)	24, 2092, 3669
LweI	GCATC	7 cut(s)	30, 1745, 1906, 2226, 2530, 2766, 3390
MaeI	CTAG	7 cut(s)	456, 912, 1613, 1802, 2444, 3633, 3702
MfeI	CAATTG	2 cut(s)	725, 749
MflI	RGATCY	3 cut(s)	1444, 1608, 2894
MhlI	GDGCHC	3 cut(s)	1006, 3316, 3555
MlyI	GAGTC	3 cut(s)	476, 1608, 3040
MmeI	TCCRAC	3 cut(s)	424, 1737, 3339
Mph1103I	ATGCAT	2 cut(s)	1038, 2707
MroXI	GAANNNNTTC	2 cut(s)	2094, 3132
MslI	CAYNNNNRTG	1 cut(s)	700
MspA1I	CMGCKG	1 cut(s)	1248
MspCI	CTTAAG	2 cut(s)	2116, 3371
MspI	CCGG	4 cut(s)	1678, 2018, 2163, 2793
MspR9I	CCNGG	5 cut(s)	1580, 2013, 2222, 2794, 3487
MunI	CAATTG	2 cut(s)	725, 749
Mva1269I	GAATGC	4 cut(s)	1036, 1664, 1978, 2094
MvaI	CCWGG	4 cut(s)	1580, 2013, 2222, 3487
MwoI	GCNNNNNNNGC	4 cut(s)	436, 688, 697, 3565
NciI	CCSGG	1 cut(s)	2794
NcoI	CCATGG	1 cut(s)	1414
NdeI	CATATG	1 cut(s)	2136
NlaIV	GGNNCC	6 cut(s)	384, 1446, 1610, 1629, 2325, 3620
NmuCI	GTSAC	4 cut(s)	1492, 1600, 3481, 3635
NsbI	TGCGCA	1 cut(s)	438
NsiI	ATGCAT	2 cut(s)	1038, 2707
NspV	TTCGAA	1 cut(s)	900
PagI	TCATGA	5 cut(s)	1134, 1666, 2619, 2746, 3061
PaqCI	CACCTGC	1 cut(s)	3461
PciSI	GCTCTTC	1 cut(s)	1060
PctI	GAATGC	4 cut(s)	1036, 1664, 1978, 2094
PdmI	GAANNNNTTC	2 cut(s)	2094, 3132
PfoI	TCCNGGA	1 cut(s)	2220
PinAI	ACCGGT	1 cut(s)	2162
PkrI	GCNGC	3 cut(s)	14, 2082, 3659
PleI	GAGTC	3 cut(s)	475, 1607, 3039
PpsI	GAGTC	3 cut(s)	475, 1607, 3039
PshBI	ATTAAT	2 cut(s)	2351, 3510
PsiI	TTATAA	2 cut(s)	2238, 2984
Psp124BI	GAGCTC	1 cut(s)	3555
Psp6I	CCWGG	4 cut(s)	1578, 2011, 2220, 3485
PspEI	GGTNACC	2 cut(s)	1120, 2007
PspFI	CCCAGC	2 cut(s)	1019, 1280
PspGI	CCWGG	4 cut(s)	1578, 2011, 2220, 3485
PspN4I	GGNNCC	6 cut(s)	384, 1446, 1610, 1629, 2325, 3620
PspPI	GGNCC	4 cut(s)	651, 2015, 2323, 3403
PsuI	RGATCY	3 cut(s)	1444, 1608, 2894
RsaI	GTAC	5 cut(s)	58, 1228, 1629, 2880, 2930
RsaNI	GTAC	5 cut(s)	57, 1227, 1628, 2879, 2929
RseI	CAYNNNNRTG	1 cut(s)	700
SacI	GAGCTC	1 cut(s)	3555
SalI	GTCGAC	1 cut(s)	1330
SapI	GCTCTTC	1 cut(s)	1060
SatI	GCNGC	3 cut(s)	13, 2081, 3658
Sau96I	GGNCC	4 cut(s)	651, 2015, 2323, 3403
SchI	GAGTC	3 cut(s)	476, 1608, 3040
ScrFI	CCNGG	5 cut(s)	1580, 2013, 2222, 2794, 3487
SduI	GDGCHC	3 cut(s)	1006, 3316, 3555
SfaNI	GCATC	7 cut(s)	30, 1745, 1906, 2226, 2530, 2766, 3390
SfcI	CTRYAG	1 cut(s)	2507
SfuI	TTCGAA	1 cut(s)	900
SinI	GGWCC	1 cut(s)	3403
SmiMI	CAYNNNNRTG	1 cut(s)	700
SmlI	CTYRAG	5 cut(s)	905, 1960, 2116, 2971, 3371
SmoI	CTYRAG	5 cut(s)	905, 1960, 2116, 2971, 3371
SsiI	CCGC	6 cut(s)	373, 1248, 1448, 2527, 2659, 2965
SspI	AATATT	4 cut(s)	272, 1987, 2245, 2767
SspMI	CTAG	7 cut(s)	456, 912, 1613, 1802, 2444, 3633, 3702
SstI	GAGCTC	1 cut(s)	3555
StyD4I	CCNGG	5 cut(s)	1578, 2011, 2220, 2792, 3485
StyI	CCWWGG	5 cut(s)	1414, 1752, 3021, 3316, 3717
TaaI	ACNGT	5 cut(s)	2293, 2303, 2344, 3201, 3481
TaqI	TCGA	7 cut(s)	670, 760, 900, 1331, 2909, 3384, 3594
TatI	WGTACW	2 cut(s)	2878, 2928
TscAI	CASTG	8 cut(s)	140, 437, 707, 1609, 1930, 2082, 2298, 3486
TseFI	GTSAC	4 cut(s)	1492, 1600, 3481, 3635
TseI	GCWGC	3 cut(s)	12, 2080, 3657
Tsp45I	GTSAC	4 cut(s)	1492, 1600, 3481, 3635
TspDTI	ATGAA	9 cut(s)	147, 945, 1395, 1683, 2267, 2690, 2871, 3050, 3335
TspGWI	ACGGA	1 cut(s)	2016
TspRI	CASTG	8 cut(s)	140, 437, 707, 1609, 1930, 2082, 2298, 3486
Vha464I	CTTAAG	2 cut(s)	2116, 3371
VpaK11BI	GGWCC	1 cut(s)	3403
VspI	ATTAAT	2 cut(s)	2351, 3510
XapI	RAATTY	7 cut(s)	480, 874, 1095, 1373, 2272, 2317, 2547
XbaI	TCTAGA	1 cut(s)	2443
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1462
XmiI	GTMKAC	1 cut(s)	1331
XmnI	GAANNNNTTC	2 cut(s)	2094, 3132
XspI	CTAG	7 cut(s)	456, 912, 1613, 1802, 2444, 3633, 3702
Zsp2I	ATGCAT	2 cut(s)	1038, 2707

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.