MD09G1140000.v1.1

Choline-phosphate cytidylyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0001730
Physical Location & Seq
Forward (+)
10885387 .. 10888936
3550 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1140000.v1.1.491

Sequence Viewer

Length: 1029 bp
ATGGCAGAGGTCGAGCCCAATCATACTAACGGCACCAGCAACGGCAGTACCAATTCGAATTCCAACCCCGCCGATCAGCCGGACCGCCTTGTTCGTGTCTACGCCGATGGGATCTACGATCTCTTCCACTTCGGCCACGCTCGCTCGCTCGAGCAGGCCAAGAAATCGTTCCCTAACACCTATCTGCTCGTCGGTTGTTGTAATGATGAAACCACTCACAAGTTCAAAGGCAAAACTGTCATGACAGAGTCTGAACGTTATGAATCTCTTCGCCATTGCAAGTGGGTAGATGAAGTCATTCCTGATGCACCTTGGGTGATCAATCAAGAATTTCTTGACAAGCATGAGATTGACTTTGTAGCCCATGACTCTCTACCTTATGCTGATGCAAGTGGAGCTGGGAAAGATGTGTACGAATTTGTCAAAGCCGTGGGAAGGTTTAAGGAAACAAAGAGAACTGATGGAATTTCAACATCGGACATTATAATGAGGATTGTAAAAGATTATAACCAGTATGTGTTGCGTAACTTGGATCGTGGATATACAAGAAAAGAGCTTGGTGTTAGTTTTGTGAAGGAAAAGCGATTAAGGGTGAACTTGAGATTGAAGAAGTTACAAGAGAAAGTGAAGGAACATCAAGAAAAAGTTGGAGAGAAGTTTCAAACTGTTGCTATGCATCGTAATGAATGGGTAGAAAATGCTGATCGCTGGGTTGCTGGATTTCTTGAGATGTTTGAAGAAGGTTGTCATAAAATGGGGACAGCCATCAGAGATCGAATTCAAGAGAGCTTACGAGGGCAGCAGTCGGGAGAAAAATATCTGCTGGAAAATGGTAAGGTGGACAGTGATGATGGACAAGAATATTACGATGAGGATGATGACGAAGAATACTATGATGATGAAGAATACTATGACGATGAAGAATATTATCAAGAGGTATACGGGAAAGATGGGAACAATGAGAACGAAAAGGAGAACGAAAAGGAGAAAAAGAAGGATGAGAAAGAAACGAATGAGAAATTAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00440 ko00564 ko01100 ko05231 map00440 map00564 map01100 map05231
Pfam Domains
Protein Families
CTP_transf_like

Protein Analysis

343

Amino Acids

40.02

Weight (kDa)

4.99

Isoelectric Point (pI)

28.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CTP_transf_like PF01467 34 - 163 1.5e-32 Cytidylyltransferase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 485, 507
AccB1I GGYRCC 1 cut(s) 32
AccI GTMKAC 2 cut(s) 99, 939
AciI CCGC 2 cut(s) 69, 85
AclI AACGTT 1 cut(s) 256
AclWI GGATC 2 cut(s) 119, 540
AcoI YGGCCR 1 cut(s) 133
AcsI RAATTY 5 cut(s) 58, 329, 416, 465, 777
AfaI GTAC 2 cut(s) 49, 413
AfiI CCNNNNNNNGG 1 cut(s) 435
AgsI TTSAA 6 cut(s) 226, 471, 607, 662, 737, 782
AjuI GAANNNNNNNTTGG 2 cut(s) 152, 184
AluBI AGCT 3 cut(s) 398, 556, 789
AluI AGCT 3 cut(s) 398, 556, 789
AlwI GGATC 2 cut(s) 119, 540
AlwNI CAGNNNCTG 1 cut(s) 251
Ama87I CYCGRG 1 cut(s) 149
AoxI GGCC 2 cut(s) 133, 156
ApeKI GCWGC 1 cut(s) 799
ApoI RAATTY 5 cut(s) 58, 329, 416, 465, 777
Asp700I GAANNNNTTC 3 cut(s) 167, 267, 297
AspS9I GGNCC 1 cut(s) 82
AsuHPI GGTGA 2 cut(s) 328, 604
AsuII TTCGAA 1 cut(s) 56
AvaI CYCGRG 1 cut(s) 149
AvaII GGWCC 1 cut(s) 82
BaeI ACNNNNGTAYC 2 cut(s) 31, 64
BanI GGYRCC 1 cut(s) 32
BanII GRGCYC 1 cut(s) 18
BbvI GCAGC 1 cut(s) 811
BccI CCATC 5 cut(s) 101, 455, 773, 845, 944
BceAI ACGGC 3 cut(s) 46, 58, 413
BclI TGATCA 1 cut(s) 318
BisI GCNGC 1 cut(s) 800
BlsI GCNGC 1 cut(s) 801
Bme18I GGWCC 1 cut(s) 82
BmeT110I CYCGRG 1 cut(s) 149
BmgT120I GGNCC 1 cut(s) 82
BmiI GGNNCC 1 cut(s) 34
BmsI GCATC 3 cut(s) 295, 376, 685
Bpu14I TTCGAA 1 cut(s) 56
BpuEI CTTGAG 2 cut(s) 619, 746
BsaJI CCNNGG 2 cut(s) 311, 429
Bsc4I CCNNNNNNNGG 1 cut(s) 435
Bse1I ACTGG 1 cut(s) 511
Bse3DI GCAATG 1 cut(s) 274
BseDI CCNNGG 2 cut(s) 311, 429
BseGI GGATG 2 cut(s) 880, 1003
BseLI CCNNNNNNNGG 1 cut(s) 435
BseMI GCAATG 1 cut(s) 274
BseNI ACTGG 1 cut(s) 511
BseXI GCAGC 1 cut(s) 811
BseYI CCCAGC 2 cut(s) 398, 708
BshFI GGCC 2 cut(s) 135, 158
BshNI GGYRCC 1 cut(s) 32
BsiHKCI CYCGRG 1 cut(s) 149
BsiSI CCGG 1 cut(s) 80
BslFI GGGAC 1 cut(s) 772
BslI CCNNNNNNNGG 1 cut(s) 435
BsmFI GGGAC 1 cut(s) 772
BsnI GGCC 2 cut(s) 135, 158
BsoBI CYCGRG 1 cut(s) 149
Bsp119I TTCGAA 1 cut(s) 56
Bsp1286I GDGCHC 1 cut(s) 18
Bsp143I GATC 7 cut(s) 73, 111, 118, 318, 532, 703, 772
BspACI CCGC 2 cut(s) 69, 85
BspANI GGCC 2 cut(s) 135, 158
BspHI TCATGA 1 cut(s) 240
BspLI GGNNCC 1 cut(s) 34
BspPI GGATC 2 cut(s) 119, 540
BspT104I TTCGAA 1 cut(s) 56
BspT107I GGYRCC 1 cut(s) 32
BsrDI GCAATG 1 cut(s) 274
BsrI ACTGG 1 cut(s) 511
BssECI CCNNGG 2 cut(s) 311, 429
BssMI GATC 7 cut(s) 73, 111, 118, 318, 532, 703, 772
BssNAI GTATAC 1 cut(s) 940
BssT1I CCWWGG 1 cut(s) 311
Bst1107I GTATAC 1 cut(s) 940
Bst4CI ACNGT 3 cut(s) 238, 667, 845
Bst6I CTCTTC 2 cut(s) 128, 273
BstBI TTCGAA 1 cut(s) 56
BstC8I GCNNGC 3 cut(s) 142, 146, 156
BstDSI CCRYGG 1 cut(s) 429
BstF5I GGATG 2 cut(s) 880, 1003
BstKTI GATC 7 cut(s) 76, 114, 121, 321, 535, 706, 775
BstMBI GATC 7 cut(s) 73, 111, 118, 318, 532, 703, 772
BstMWI GCNNNNNNNGC 2 cut(s) 141, 395
BstV1I GCAGC 1 cut(s) 811
BstX2I RGATCY 1 cut(s) 111
BstYI RGATCY 1 cut(s) 111
BstZ17I GTATAC 1 cut(s) 940
BsuRI GGCC 2 cut(s) 135, 158
BtgI CCRYGG 1 cut(s) 429
BtsCI GGATG 2 cut(s) 880, 1003
BtsIMutI CAGTG 1 cut(s) 850
Cac8I GCNNGC 3 cut(s) 142, 146, 156
CaiI CAGNNNCTG 1 cut(s) 251
CciI TCATGA 1 cut(s) 240
Cfr13I GGNCC 1 cut(s) 82
CpoI CGGWCCG 1 cut(s) 82
Csp6I GTAC 2 cut(s) 48, 412
CspI CGGWCCG 1 cut(s) 82
CviAII CATG 3 cut(s) 241, 344, 365
CviQI GTAC 2 cut(s) 48, 412
DpnI GATC 7 cut(s) 75, 113, 120, 320, 534, 705, 774
DpnII GATC 7 cut(s) 73, 111, 118, 318, 532, 703, 772
EaeI YGGCCR 1 cut(s) 133
Eam1104I CTCTTC 2 cut(s) 128, 273
EarI CTCTTC 2 cut(s) 128, 273
Eco130I CCWWGG 1 cut(s) 311
Eco24I GRGCYC 1 cut(s) 18
Eco47I GGWCC 1 cut(s) 82
Eco88I CYCGRG 1 cut(s) 149
EcoRI GAATTC 2 cut(s) 58, 777
EcoT14I CCWWGG 1 cut(s) 311
EcoT22I ATGCAT 1 cut(s) 678
EcoT38I GRGCYC 1 cut(s) 18
ErhI CCWWGG 1 cut(s) 311
FaeI CATG 3 cut(s) 244, 347, 368
FalI AAGNNNNNCTT 2 cut(s) 318, 350
FaqI GGGAC 1 cut(s) 772
FatI CATG 3 cut(s) 240, 343, 364
FauI CCCGC 1 cut(s) 76
FbaI TGATCA 1 cut(s) 318
FblI GTMKAC 2 cut(s) 99, 939
Fnu4HI GCNGC 1 cut(s) 800
FokI GGATG 2 cut(s) 887, 1010
FriOI GRGCYC 1 cut(s) 18
Fsp4HI GCNGC 1 cut(s) 800
GluI GCNGC 1 cut(s) 800
GsaI CCCAGC 2 cut(s) 402, 712
HaeIII GGCC 2 cut(s) 135, 158
HapII CCGG 1 cut(s) 80
Hin1II CATG 3 cut(s) 244, 347, 368
HinfI GANTC 3 cut(s) 248, 263, 368
HpaII CCGG 1 cut(s) 80
HphI GGTGA 2 cut(s) 328, 604
Hpy166II GTNNAC 5 cut(s) 100, 412, 595, 841, 940
Hpy188I TCNGA 3 cut(s) 253, 478, 770
Hpy188III TCNNGA 9 cut(s) 241, 302, 326, 335, 638, 725, 782, 807, 932
Hpy8I GTNNAC 5 cut(s) 100, 412, 595, 841, 940
Hpy99I CGWCG 1 cut(s) 194
HpyAV CCTTC 5 cut(s) 429, 568, 622, 734, 988
HpyCH4III ACNGT 3 cut(s) 238, 667, 845
HpyCH4IV ACGT 1 cut(s) 256
HpyCH4V TGCA 4 cut(s) 279, 308, 389, 676
HpyF10VI GCNNNNNNNGC 2 cut(s) 141, 395
HpySE526I ACGT 1 cut(s) 256
Hsp92II CATG 3 cut(s) 244, 347, 368
Ksp22I TGATCA 1 cut(s) 318
Kzo9I GATC 7 cut(s) 73, 111, 118, 318, 532, 703, 772
LmnI GCTCC 1 cut(s) 395
LpnPI CCDG 9 cut(s) 49, 93, 140, 315, 384, 524, 694, 702, 809
Lsp1109I GCAGC 1 cut(s) 811
LweI GCATC 3 cut(s) 295, 376, 685
MaeII ACGT 1 cut(s) 256
MaeIII GTNAC 2 cut(s) 524, 612
MalI GATC 7 cut(s) 75, 113, 120, 320, 534, 705, 774
MboI GATC 7 cut(s) 73, 111, 118, 318, 532, 703, 772
MboII GAAGA 7 cut(s) 115, 260, 619, 749, 896, 914, 932
MflI RGATCY 1 cut(s) 111
MhlI GDGCHC 1 cut(s) 18
MluCI AATT 7 cut(s) 52, 58, 329, 416, 465, 777, 1019
MlyI GAGTC 2 cut(s) 257, 362
MmeI TCCRAC 2 cut(s) 87, 628
MnlI CCTC 4 cut(s) 483, 788, 865, 928
Mph1103I ATGCAT 1 cut(s) 678
MroXI GAANNNNTTC 3 cut(s) 167, 267, 297
MseI TTAA 3 cut(s) 441, 587, 1022
MslI CAYNNNNRTG 2 cut(s) 485, 681
MspI CCGG 1 cut(s) 80
MwoI GCNNNNNNNGC 2 cut(s) 141, 395
NdeII GATC 7 cut(s) 73, 111, 118, 318, 532, 703, 772
NlaIII CATG 3 cut(s) 244, 347, 368
NlaIV GGNNCC 1 cut(s) 34
NsiI ATGCAT 1 cut(s) 678
NspV TTCGAA 1 cut(s) 56
PaeR7I CTCGAG 1 cut(s) 149
PagI TCATGA 1 cut(s) 240
PdmI GAANNNNTTC 3 cut(s) 167, 267, 297
PfeI GAWTC 1 cut(s) 263
PflFI GACNNNGTC 1 cut(s) 247
PkrI GCNGC 1 cut(s) 801
PleI GAGTC 2 cut(s) 256, 362
PpsI GAGTC 2 cut(s) 256, 362
PsiI TTATAA 2 cut(s) 485, 507
Psp1406I AACGTT 1 cut(s) 256
PspFI CCCAGC 2 cut(s) 398, 708
PspN4I GGNNCC 1 cut(s) 34
PspPI GGNCC 1 cut(s) 82
PspXI VCTCGAGB 1 cut(s) 149
PstNI CAGNNNCTG 1 cut(s) 251
PsuI RGATCY 1 cut(s) 111
PsyI GACNNNGTC 1 cut(s) 247
RsaI GTAC 2 cut(s) 49, 413
RsaNI GTAC 2 cut(s) 48, 412
RseI CAYNNNNRTG 2 cut(s) 485, 681
Rsr2I CGGWCCG 1 cut(s) 82
RsrII CGGWCCG 1 cut(s) 82
SaqAI TTAA 3 cut(s) 441, 587, 1022
SatI GCNGC 1 cut(s) 800
Sau3AI GATC 7 cut(s) 73, 111, 118, 318, 532, 703, 772
Sau96I GGNCC 1 cut(s) 82
SchI GAGTC 2 cut(s) 257, 362
SduI GDGCHC 1 cut(s) 18
SfaNI GCATC 3 cut(s) 295, 376, 685
Sfr274I CTCGAG 1 cut(s) 149
SfuI TTCGAA 1 cut(s) 56
SinI GGWCC 1 cut(s) 82
SlaI CTCGAG 1 cut(s) 149
SmiMI CAYNNNNRTG 2 cut(s) 485, 681
SmlI CTYRAG 3 cut(s) 149, 598, 725
SmoI CTYRAG 3 cut(s) 149, 598, 725
Sse9I AATT 7 cut(s) 52, 58, 329, 416, 465, 777, 1019
SsiI CCGC 2 cut(s) 69, 85
SspI AATATT 2 cut(s) 863, 926
StyI CCWWGG 1 cut(s) 311
TaaI ACNGT 3 cut(s) 238, 667, 845
TaiI ACGT 1 cut(s) 259
TaqI TCGA 4 cut(s) 12, 56, 150, 775
TasI AATT 7 cut(s) 52, 58, 329, 416, 465, 777, 1019
TfiI GAWTC 1 cut(s) 263
Tru1I TTAA 3 cut(s) 441, 587, 1022
Tru9I TTAA 3 cut(s) 441, 587, 1022
TscAI CASTG 1 cut(s) 850
TseI GCWGC 1 cut(s) 799
TspDTI ATGAA 6 cut(s) 222, 276, 306, 699, 915, 933
TspRI CASTG 1 cut(s) 850
Tth111I GACNNNGTC 1 cut(s) 247
VpaK11BI GGWCC 1 cut(s) 82
XapI RAATTY 5 cut(s) 58, 329, 416, 465, 777
XhoI CTCGAG 1 cut(s) 149
XmiI GTMKAC 2 cut(s) 99, 939
XmnI GAANNNNTTC 3 cut(s) 167, 267, 297
Zsp2I ATGCAT 1 cut(s) 678
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.