MD17G1129400.v1.1

Choline-phosphate cytidylyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0001730
Physical Location & Seq
Forward (+)
11389344 .. 11392563
3220 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1129400.v1.1.491

Sequence Viewer

Length: 813 bp
ATGGCAGAGGTCGAGCCCAAAGATACTAACGGCACTAGCAACGGTAGTGCCAATTCGAATTCGAACCCCGCCGATCAGCCGGACCGCCTTGTTCGTGTCTACGCCGATGGGATCTACGATCTCTTCCACTTCGGCCACGCTCGCTCGCTCGAGCAGGCCAAGAAATCGTTCCCTAACACCTATCTGCTCGTCGGTTGTTGTAATGATGAAACCACTCACAAGTTTAAAGGGAAAACTGTTATGACAGAGTCTGAACGTTATGAATCTCTTCGCCATTGCAAGTGGGTAGATGAAGTCATTCCTGATGCTCCTTGGGTGATCAATCAAGAATTTCTTGACAAGCATGAGATTGGCTTTGTAGCCCATGACTCTCTACCTTATGCTGATGCAAGTGGAGCTGGGCAAGACGTGTACGAATTTGTCAAAGCCGTGGGGAGGTTTAAGGAAACAAAGAGAACTGATGGGATTTCAACTTCGGACATTATAATGAGAATTGTAAAAGATTATAACCAGTATGTGTTGCGTAACTTGGATCGTGGATATACAAGAAAAGAGCTTGGTGTTAGTTTTGTGAAGGAAAAGCGATTAAGGGTGAACATGAGATTGAAGAAGTTTCAAGAGAAAGTGAAGGAACATCAAGAGAAGGTAGGAGAGAAGATTCAAACTGTTGCCATGCATCGTAATGAATGGGTTGAAAATGCTGATCGCTGGGTTGCTGGATTTCTTGAGATGTTTGAAGAAGGTTGCCATAAAATGGTAAAGACAATATATGCATTACCACTTACATATAGACTGATATTTCTACAAGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00440 ko00564 ko01100 ko05231 map00440 map00564 map01100 map05231
Pfam Domains
Protein Families
CTP_transf_like

Protein Analysis

271

Amino Acids

31.24

Weight (kDa)

7.12

Isoelectric Point (pI)

25.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CTP_transf_like PF01467 34 - 163 3.6e-32 Cytidylyltransferase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 485, 507
AccB7I CCANNNNNTGG 1 cut(s) 754
AccI GTMKAC 1 cut(s) 99
AciI CCGC 2 cut(s) 69, 85
AclI AACGTT 1 cut(s) 256
AclWI GGATC 2 cut(s) 119, 540
AcoI YGGCCR 1 cut(s) 133
AcsI RAATTY 3 cut(s) 58, 329, 416
AfaI GTAC 1 cut(s) 413
AfiI CCNNNNNNNGG 2 cut(s) 435, 754
AflIII ACRYGT 1 cut(s) 408
AgsI TTSAA 6 cut(s) 471, 607, 617, 662, 695, 737
AjiI CACGTC 1 cut(s) 409
AjuI GAANNNNNNNTTGG 2 cut(s) 152, 184
AluBI AGCT 2 cut(s) 398, 556
AluI AGCT 2 cut(s) 398, 556
AlwI GGATC 2 cut(s) 119, 540
AlwNI CAGNNNCTG 1 cut(s) 251
Ama87I CYCGRG 1 cut(s) 149
AoxI GGCC 2 cut(s) 133, 156
ApoI RAATTY 3 cut(s) 58, 329, 416
Asp700I GAANNNNTTC 3 cut(s) 167, 267, 297
AspS9I GGNCC 1 cut(s) 82
AsuHPI GGTGA 2 cut(s) 328, 604
AsuII TTCGAA 2 cut(s) 56, 62
AvaI CYCGRG 1 cut(s) 149
AvaII GGWCC 1 cut(s) 82
BanII GRGCYC 1 cut(s) 18
BccI CCATC 2 cut(s) 101, 455
BceAI ACGGC 2 cut(s) 46, 413
BclI TGATCA 1 cut(s) 318
BfaI CTAG 1 cut(s) 36
Bme18I GGWCC 1 cut(s) 82
BmeT110I CYCGRG 1 cut(s) 149
BmgBI CACGTC 1 cut(s) 409
BmgT120I GGNCC 1 cut(s) 82
BmsI GCATC 3 cut(s) 295, 376, 685
Bpu14I TTCGAA 2 cut(s) 56, 62
BpuEI CTTGAG 1 cut(s) 746
BsaJI CCNNGG 2 cut(s) 311, 429
Bsc4I CCNNNNNNNGG 2 cut(s) 435, 754
Bse1I ACTGG 1 cut(s) 511
Bse3DI GCAATG 1 cut(s) 274
BseDI CCNNGG 2 cut(s) 311, 429
BseLI CCNNNNNNNGG 2 cut(s) 435, 754
BseMI GCAATG 1 cut(s) 274
BseNI ACTGG 1 cut(s) 511
BseYI CCCAGC 2 cut(s) 398, 708
BshFI GGCC 2 cut(s) 135, 158
BsiHKCI CYCGRG 1 cut(s) 149
BsiSI CCGG 1 cut(s) 80
BslI CCNNNNNNNGG 2 cut(s) 435, 754
BsnI GGCC 2 cut(s) 135, 158
BsoBI CYCGRG 1 cut(s) 149
Bsp119I TTCGAA 2 cut(s) 56, 62
Bsp1286I GDGCHC 1 cut(s) 18
Bsp143I GATC 6 cut(s) 73, 111, 118, 318, 532, 703
BspACI CCGC 2 cut(s) 69, 85
BspANI GGCC 2 cut(s) 135, 158
BspPI GGATC 2 cut(s) 119, 540
BspT104I TTCGAA 2 cut(s) 56, 62
BsrDI GCAATG 1 cut(s) 274
BsrI ACTGG 1 cut(s) 511
BssECI CCNNGG 2 cut(s) 311, 429
BssMI GATC 6 cut(s) 73, 111, 118, 318, 532, 703
BssT1I CCWWGG 1 cut(s) 311
Bst4CI ACNGT 3 cut(s) 44, 238, 667
Bst6I CTCTTC 2 cut(s) 128, 273
BstBI TTCGAA 2 cut(s) 56, 62
BstC8I GCNNGC 3 cut(s) 142, 146, 156
BstDSI CCRYGG 1 cut(s) 429
BstKTI GATC 6 cut(s) 76, 114, 121, 321, 535, 706
BstMBI GATC 6 cut(s) 73, 111, 118, 318, 532, 703
BstMWI GCNNNNNNNGC 2 cut(s) 141, 395
BstX2I RGATCY 1 cut(s) 111
BstYI RGATCY 1 cut(s) 111
BsuRI GGCC 2 cut(s) 135, 158
BtgI CCRYGG 1 cut(s) 429
BtrI CACGTC 1 cut(s) 409
Cac8I GCNNGC 3 cut(s) 142, 146, 156
CaiI CAGNNNCTG 1 cut(s) 251
Cfr13I GGNCC 1 cut(s) 82
CpoI CGGWCCG 1 cut(s) 82
Csp6I GTAC 1 cut(s) 412
CspI CGGWCCG 1 cut(s) 82
CviAII CATG 4 cut(s) 344, 365, 598, 673
CviJI RGCY 9 cut(s) 16, 79, 135, 158, 354, 362, 398, 428, 556
CviKI_1 RGCY 9 cut(s) 16, 79, 135, 158, 354, 362, 398, 428, 556
CviQI GTAC 1 cut(s) 412
DpnI GATC 6 cut(s) 75, 113, 120, 320, 534, 705
DpnII GATC 6 cut(s) 73, 111, 118, 318, 532, 703
DraI TTTAAA 1 cut(s) 226
EaeI YGGCCR 1 cut(s) 133
Eam1104I CTCTTC 2 cut(s) 128, 273
EarI CTCTTC 2 cut(s) 128, 273
Eco130I CCWWGG 1 cut(s) 311
Eco24I GRGCYC 1 cut(s) 18
Eco47I GGWCC 1 cut(s) 82
Eco88I CYCGRG 1 cut(s) 149
EcoRI GAATTC 1 cut(s) 58
EcoT14I CCWWGG 1 cut(s) 311
EcoT22I ATGCAT 2 cut(s) 678, 775
EcoT38I GRGCYC 1 cut(s) 18
ErhI CCWWGG 1 cut(s) 311
FaeI CATG 4 cut(s) 347, 368, 601, 676
FalI AAGNNNNNCTT 2 cut(s) 318, 350
FatI CATG 4 cut(s) 343, 364, 597, 672
FauI CCCGC 1 cut(s) 76
FbaI TGATCA 1 cut(s) 318
FblI GTMKAC 1 cut(s) 99
FriOI GRGCYC 1 cut(s) 18
FspBI CTAG 1 cut(s) 36
GsaI CCCAGC 2 cut(s) 402, 712
HaeIII GGCC 2 cut(s) 135, 158
HapII CCGG 1 cut(s) 80
Hin1II CATG 4 cut(s) 347, 368, 601, 676
HinfI GANTC 4 cut(s) 248, 263, 368, 658
HpaII CCGG 1 cut(s) 80
HphI GGTGA 2 cut(s) 328, 604
Hpy166II GTNNAC 3 cut(s) 100, 412, 595
Hpy188I TCNGA 2 cut(s) 253, 478
Hpy188III TCNNGA 6 cut(s) 302, 326, 335, 617, 638, 725
Hpy8I GTNNAC 3 cut(s) 100, 412, 595
Hpy99I CGWCG 1 cut(s) 194
HpyAV CCTTC 4 cut(s) 568, 622, 637, 734
HpyCH4III ACNGT 3 cut(s) 44, 238, 667
HpyCH4IV ACGT 2 cut(s) 256, 408
HpyCH4V TGCA 4 cut(s) 279, 389, 676, 773
HpyF10VI GCNNNNNNNGC 2 cut(s) 141, 395
HpySE526I ACGT 2 cut(s) 256, 408
Hsp92II CATG 4 cut(s) 347, 368, 601, 676
Ksp22I TGATCA 1 cut(s) 318
Kzo9I GATC 6 cut(s) 73, 111, 118, 318, 532, 703
LmnI GCTCC 2 cut(s) 313, 395
LpnPI CCDG 7 cut(s) 93, 140, 315, 384, 524, 694, 702
LweI GCATC 3 cut(s) 295, 376, 685
MaeI CTAG 1 cut(s) 36
MaeII ACGT 2 cut(s) 256, 408
MaeIII GTNAC 1 cut(s) 524
MalI GATC 6 cut(s) 75, 113, 120, 320, 534, 705
MboI GATC 6 cut(s) 73, 111, 118, 318, 532, 703
MboII GAAGA 5 cut(s) 115, 260, 619, 667, 749
MflI RGATCY 1 cut(s) 111
MhlI GDGCHC 1 cut(s) 18
MluCI AATT 5 cut(s) 52, 58, 329, 416, 492
MlyI GAGTC 2 cut(s) 257, 362
MnlI CCTC 1 cut(s) 429
Mph1103I ATGCAT 2 cut(s) 678, 775
MroXI GAANNNNTTC 3 cut(s) 167, 267, 297
MseI TTAA 3 cut(s) 225, 441, 587
MslI CAYNNNNRTG 2 cut(s) 485, 681
MspI CCGG 1 cut(s) 80
MwoI GCNNNNNNNGC 2 cut(s) 141, 395
NdeII GATC 6 cut(s) 73, 111, 118, 318, 532, 703
NlaIII CATG 4 cut(s) 347, 368, 601, 676
NsiI ATGCAT 2 cut(s) 678, 775
NspV TTCGAA 2 cut(s) 56, 62
PaeR7I CTCGAG 1 cut(s) 149
PdmI GAANNNNTTC 3 cut(s) 167, 267, 297
PfeI GAWTC 2 cut(s) 263, 658
PflFI GACNNNGTC 1 cut(s) 247
PflMI CCANNNNNTGG 1 cut(s) 754
PleI GAGTC 2 cut(s) 256, 362
PpsI GAGTC 2 cut(s) 256, 362
PsiI TTATAA 2 cut(s) 485, 507
Psp1406I AACGTT 1 cut(s) 256
PspFI CCCAGC 2 cut(s) 398, 708
PspPI GGNCC 1 cut(s) 82
PspXI VCTCGAGB 1 cut(s) 149
PstNI CAGNNNCTG 1 cut(s) 251
PsuI RGATCY 1 cut(s) 111
PsyI GACNNNGTC 1 cut(s) 247
RsaI GTAC 1 cut(s) 413
RsaNI GTAC 1 cut(s) 412
RseI CAYNNNNRTG 2 cut(s) 485, 681
Rsr2I CGGWCCG 1 cut(s) 82
RsrII CGGWCCG 1 cut(s) 82
SaqAI TTAA 3 cut(s) 225, 441, 587
Sau3AI GATC 6 cut(s) 73, 111, 118, 318, 532, 703
Sau96I GGNCC 1 cut(s) 82
SchI GAGTC 2 cut(s) 257, 362
SduI GDGCHC 1 cut(s) 18
SfaNI GCATC 3 cut(s) 295, 376, 685
Sfr274I CTCGAG 1 cut(s) 149
SfuI TTCGAA 2 cut(s) 56, 62
SinI GGWCC 1 cut(s) 82
SlaI CTCGAG 1 cut(s) 149
SmiMI CAYNNNNRTG 2 cut(s) 485, 681
SmlI CTYRAG 2 cut(s) 149, 725
SmoI CTYRAG 2 cut(s) 149, 725
Sse9I AATT 5 cut(s) 52, 58, 329, 416, 492
SsiI CCGC 2 cut(s) 69, 85
SspMI CTAG 1 cut(s) 36
StyI CCWWGG 1 cut(s) 311
TaaI ACNGT 3 cut(s) 44, 238, 667
TaiI ACGT 2 cut(s) 259, 411
TaqI TCGA 4 cut(s) 12, 56, 62, 150
TasI AATT 5 cut(s) 52, 58, 329, 416, 492
TfiI GAWTC 2 cut(s) 263, 658
Tru1I TTAA 3 cut(s) 225, 441, 587
Tru9I TTAA 3 cut(s) 225, 441, 587
TspDTI ATGAA 4 cut(s) 222, 276, 306, 699
Tth111I GACNNNGTC 1 cut(s) 247
Van91I CCANNNNNTGG 1 cut(s) 754
VpaK11BI GGWCC 1 cut(s) 82
XapI RAATTY 3 cut(s) 58, 329, 416
XhoI CTCGAG 1 cut(s) 149
XmiI GTMKAC 1 cut(s) 99
XmnI GAANNNNTTC 3 cut(s) 167, 267, 297
XspI CTAG 1 cut(s) 36
Zsp2I ATGCAT 2 cut(s) 678, 775
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.