MD10G1228600.v1.1

Cholinephosphate cytidylyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0001730
Physical Location & Seq
Reverse (-)
32525426 .. 32528883
3458 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1228600.v1.1.491

Sequence Viewer

Length: 870 bp
ATGGAGGAAGAGAAGCAGCAGAGTATTAAGGAGGAGGAGGAGGAGGAGCGTCCAGTGAGAGTATATGCAGACGGCATTTATGATCTCTTTCACTTCGGCCATGCCAGTTCTCTCGAGCAAGCCAAGAAGCTGTTCCCCAATACGTACCTGCTTGTTGGCTGCTGTGGTGATGAAATTACCCACAATTATAAGGGAAAAACTGTTATGACTGAGAAGGAGCGCTATGAATCGCTCCGCCATTGCAGATGGGTGGACGAAGTTATCCCAGATGCACCATGGGTGCTCTCGCAAGAGTTTCTTGACAAGCATAAGATTGACTTTGTGGCACATGACTCTCTTCCCTATGCTAATGCAAGTGGCTCTGGAAAGGATGTCTATGAATTTGTTAAGTCTGTTGGGAAGTTTAAAGAAACAAAGCGAACTGATGGGATCTCTACATCGGATCTCATAATGAGGATAGTTAAAGATTACAACCAGTATGTGATGCGTAATTTGGACCGTGGGTACACAAGAAAAGATCTAGGTGTCAGCTATGTGAGGGAAAAGCGACTGAGAGTCAACATGGGACTGAAAAGATTGCGTGAGAGGGTGAAGGAGCACCAAGAAAAAGTAGGAGAGAAGATACAAATAGTAACGCAGACTGCCACCATGCGTCATAATGAATGGGTGGAGAACGCTGACCGGCTGGTTGCTGGATTTCTGGAGAGGTTTGAAGAAGGTTTCCATAAAATGGGAACAGCCATAAGGGACCGAATTCAAAAGCGAATAAGGAAGCAGCAGTTCAGAGGGCTTTTATATGAAGAAGAAGATGATGATGATGAATTCTATGATGACTCTGAAGAAGAAAACGATGATGAAGACGAGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00440 ko00564 ko01100 ko05231 map00440 map00564 map01100 map05231
Pfam Domains
Protein Families
CTP_transf_like

Protein Analysis

290

Amino Acids

34.24

Weight (kDa)

5.26

Isoelectric Point (pI)

42.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CTP_transf_like PF01467 22 - 151 3.3e-30 Cytidylyltransferase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 189
Acc36I ACCTGC 1 cut(s) 156
AccB7I CCANNNNNTGG 1 cut(s) 730
AciI CCGC 1 cut(s) 235
AclWI GGATC 2 cut(s) 437, 450
AcoI YGGCCR 1 cut(s) 97
AcsI RAATTY 3 cut(s) 380, 753, 821
AcuI CTGAAG 1 cut(s) 858
AfaI GTAC 2 cut(s) 146, 506
AfeI AGCGCT 1 cut(s) 221
AfiI CCNNNNNNNGG 1 cut(s) 730
AgsI TTSAA 2 cut(s) 713, 758
AhdI GACNNNNNGTC 1 cut(s) 554
AjuI GAANNNNNNNTTGG 2 cut(s) 116, 148
AluBI AGCT 2 cut(s) 130, 531
AluI AGCT 2 cut(s) 130, 531
Alw21I GWGCWC 2 cut(s) 285, 600
AlwI GGATC 2 cut(s) 437, 450
Ama87I CYCGRG 1 cut(s) 113
Aor51HI AGCGCT 1 cut(s) 221
AoxI GGCC 1 cut(s) 97
ApeKI GCWGC 3 cut(s) 16, 159, 775
ApoI RAATTY 3 cut(s) 380, 753, 821
Asp700I GAANNNNTTC 1 cut(s) 131
AspLEI GCGC 1 cut(s) 222
AspS9I GGNCC 2 cut(s) 496, 748
AsuHPI GGTGA 2 cut(s) 179, 601
AvaI CYCGRG 1 cut(s) 113
AvaII GGWCC 2 cut(s) 496, 748
BbsI GAAGAC 1 cut(s) 864
Bbv12I GWGCWC 2 cut(s) 285, 600
BbvI GCAGC 3 cut(s) 28, 146, 787
BccI CCATC 2 cut(s) 240, 419
BceAI ACGGC 1 cut(s) 88
BfaI CTAG 1 cut(s) 521
BfoI RGCGCY 1 cut(s) 223
BfuAI ACCTGC 1 cut(s) 156
BglII AGATCT 1 cut(s) 517
BisI GCNGC 3 cut(s) 17, 160, 776
BlsI GCNGC 3 cut(s) 18, 161, 777
Bme18I GGWCC 2 cut(s) 496, 748
BmeRI GACNNNNNGTC 1 cut(s) 554
BmeT110I CYCGRG 1 cut(s) 113
BmgT120I GGNCC 2 cut(s) 496, 748
BmiI GGNNCC 1 cut(s) 749
BmsI GCATC 2 cut(s) 259, 474
BpiI GAAGAC 1 cut(s) 864
BpmI CTGGAG 1 cut(s) 722
BsaAI YACGTR 1 cut(s) 144
BsaJI CCNNGG 2 cut(s) 275, 499
Bsc4I CCNNNNNNNGG 1 cut(s) 730
Bse118I RCCGGY 1 cut(s) 681
Bse1I ACTGG 3 cut(s) 53, 105, 475
Bse3DI GCAATG 1 cut(s) 238
BseDI CCNNGG 2 cut(s) 275, 499
BseGI GGATG 1 cut(s) 376
BseLI CCNNNNNNNGG 1 cut(s) 730
BseMI GCAATG 1 cut(s) 238
BseMII CTCAG 2 cut(s) 201, 542
BseNI ACTGG 3 cut(s) 53, 105, 475
BseRI GAGGAG 5 cut(s) 47, 50, 53, 56, 59
BseXI GCAGC 3 cut(s) 28, 146, 787
BshFI GGCC 1 cut(s) 99
BsiHKAI GWGCWC 2 cut(s) 285, 600
BsiHKCI CYCGRG 1 cut(s) 113
BsiSI CCGG 1 cut(s) 682
BslFI GGGAC 2 cut(s) 579, 761
BslI CCNNNNNNNGG 1 cut(s) 730
BsmFI GGGAC 2 cut(s) 579, 761
BsnI GGCC 1 cut(s) 99
BsoBI CYCGRG 1 cut(s) 113
Bsp1286I GDGCHC 2 cut(s) 285, 600
Bsp143I GATC 4 cut(s) 82, 429, 442, 517
Bsp19I CCATGG 1 cut(s) 275
BspACI CCGC 1 cut(s) 235
BspANI GGCC 1 cut(s) 99
BspCNI CTCAG 2 cut(s) 202, 543
BspLI GGNNCC 1 cut(s) 749
BspMI ACCTGC 1 cut(s) 156
BspPI GGATC 2 cut(s) 437, 450
BsrDI GCAATG 1 cut(s) 238
BsrFI RCCGGY 1 cut(s) 681
BsrI ACTGG 3 cut(s) 53, 105, 475
BssAI RCCGGY 1 cut(s) 681
BssECI CCNNGG 2 cut(s) 275, 499
BssMI GATC 4 cut(s) 82, 429, 442, 517
BssT1I CCWWGG 1 cut(s) 275
Bst4CI ACNGT 2 cut(s) 202, 500
Bst6I CTCTTC 2 cut(s) 3, 342
BstBAI YACGTR 1 cut(s) 144
BstC8I GCNNGC 1 cut(s) 120
BstDEI CTNAG 2 cut(s) 210, 551
BstDSI CCRYGG 2 cut(s) 275, 499
BstF5I GGATG 1 cut(s) 376
BstH2I RGCGCY 1 cut(s) 223
BstHHI GCGC 1 cut(s) 222
BstKTI GATC 4 cut(s) 85, 432, 445, 520
BstMBI GATC 4 cut(s) 82, 429, 442, 517
BstSNI TACGTA 1 cut(s) 144
BstV1I GCAGC 3 cut(s) 28, 146, 787
BstV2I GAAGAC 1 cut(s) 864
BstX2I RGATCY 3 cut(s) 429, 442, 517
BstYI RGATCY 3 cut(s) 429, 442, 517
BsuRI GGCC 1 cut(s) 99
BtgI CCRYGG 2 cut(s) 275, 499
BtsCI GGATG 1 cut(s) 376
BtsIMutI CAGTG 1 cut(s) 60
BveI ACCTGC 1 cut(s) 156
Cac8I GCNNGC 1 cut(s) 120
CfoI GCGC 1 cut(s) 222
Cfr10I RCCGGY 1 cut(s) 681
Cfr13I GGNCC 2 cut(s) 496, 748
CseI GACGC 2 cut(s) 38, 641
Csp6I GTAC 2 cut(s) 145, 505
CviAII CATG 5 cut(s) 101, 276, 329, 562, 649
CviJI RGCY 9 cut(s) 99, 122, 130, 159, 360, 531, 685, 740, 790
CviKI_1 RGCY 9 cut(s) 99, 122, 130, 159, 360, 531, 685, 740, 790
CviQI GTAC 2 cut(s) 145, 505
DdeI CTNAG 2 cut(s) 210, 551
DpnI GATC 4 cut(s) 84, 431, 444, 519
DpnII GATC 4 cut(s) 82, 429, 442, 517
DraI TTTAAA 1 cut(s) 406
DriI GACNNNNNGTC 1 cut(s) 554
EaeI YGGCCR 1 cut(s) 97
Eam1104I CTCTTC 2 cut(s) 3, 342
Eam1105I GACNNNNNGTC 1 cut(s) 554
EarI CTCTTC 2 cut(s) 3, 342
EciI GGCGGA 1 cut(s) 224
Eco105I TACGTA 1 cut(s) 144
Eco130I CCWWGG 1 cut(s) 275
Eco47I GGWCC 2 cut(s) 496, 748
Eco47III AGCGCT 1 cut(s) 221
Eco57I CTGAAG 1 cut(s) 858
Eco88I CYCGRG 1 cut(s) 113
EcoRI GAATTC 2 cut(s) 753, 821
EcoT14I CCWWGG 1 cut(s) 275
ErhI CCWWGG 1 cut(s) 275
FaeI CATG 5 cut(s) 104, 279, 332, 565, 652
FalI AAGNNNNNCTT 4 cut(s) 282, 314, 302, 334
FaqI GGGAC 2 cut(s) 579, 761
FatI CATG 5 cut(s) 100, 275, 328, 561, 648
Fnu4HI GCNGC 3 cut(s) 17, 160, 776
FokI GGATG 1 cut(s) 383
Fsp4HI GCNGC 3 cut(s) 17, 160, 776
FspBI CTAG 1 cut(s) 521
GlaI GCGC 1 cut(s) 221
GluI GCNGC 3 cut(s) 17, 160, 776
GsuI CTGGAG 1 cut(s) 722
HaeII RGCGCY 1 cut(s) 223
HaeIII GGCC 1 cut(s) 99
HapII CCGG 1 cut(s) 682
HgaI GACGC 2 cut(s) 38, 641
HhaI GCGC 1 cut(s) 222
Hin1II CATG 5 cut(s) 104, 279, 332, 565, 652
Hin6I GCGC 1 cut(s) 220
HinP1I GCGC 1 cut(s) 220
HincII GTYRAC 1 cut(s) 559
HindII GTYRAC 1 cut(s) 559
HinfI GANTC 4 cut(s) 227, 332, 555, 833
HpaII CCGG 1 cut(s) 682
HphI GGTGA 2 cut(s) 179, 601
Hpy166II GTNNAC 3 cut(s) 253, 507, 559
Hpy188I TCNGA 3 cut(s) 442, 785, 838
Hpy188III TCNNGA 4 cut(s) 113, 299, 363, 701
Hpy8I GTNNAC 3 cut(s) 253, 507, 559
HpyAV CCTTC 3 cut(s) 208, 586, 710
HpyCH4III ACNGT 2 cut(s) 202, 500
HpyCH4IV ACGT 1 cut(s) 143
HpyCH4V TGCA 4 cut(s) 68, 243, 272, 353
HpyF3I CTNAG 2 cut(s) 210, 551
HpySE526I ACGT 1 cut(s) 143
Hsp92II CATG 5 cut(s) 104, 279, 332, 565, 652
HspAI GCGC 1 cut(s) 220
Kzo9I GATC 4 cut(s) 82, 429, 442, 517
LmnI GCTCC 4 cut(s) 46, 217, 237, 595
Lsp1109I GCAGC 3 cut(s) 28, 146, 787
LweI GCATC 2 cut(s) 259, 474
MaeI CTAG 1 cut(s) 521
MaeII ACGT 1 cut(s) 143
MaeIII GTNAC 1 cut(s) 631
MalI GATC 4 cut(s) 84, 431, 444, 519
MboI GATC 4 cut(s) 82, 429, 442, 517
MflI RGATCY 3 cut(s) 429, 442, 517
MhlI GDGCHC 2 cut(s) 285, 600
MluCI AATT 6 cut(s) 174, 184, 380, 490, 753, 821
MlyI GAGTC 3 cut(s) 326, 564, 827
MroXI GAANNNNTTC 1 cut(s) 131
MseI TTAA 4 cut(s) 27, 387, 405, 462
MspI CCGG 1 cut(s) 682
NcoI CCATGG 1 cut(s) 275
NdeII GATC 4 cut(s) 82, 429, 442, 517
NlaIII CATG 5 cut(s) 104, 279, 332, 565, 652
NlaIV GGNNCC 1 cut(s) 749
PaeR7I CTCGAG 1 cut(s) 113
PdmI GAANNNNTTC 1 cut(s) 131
PfeI GAWTC 1 cut(s) 227
PflMI CCANNNNNTGG 1 cut(s) 730
PkrI GCNGC 3 cut(s) 18, 161, 777
PleI GAGTC 3 cut(s) 326, 563, 827
PpsI GAGTC 3 cut(s) 326, 563, 827
Ppu21I YACGTR 1 cut(s) 144
PsiI TTATAA 1 cut(s) 189
PspN4I GGNNCC 1 cut(s) 749
PspPI GGNCC 2 cut(s) 496, 748
PsuI RGATCY 3 cut(s) 429, 442, 517
RsaI GTAC 2 cut(s) 146, 506
RsaNI GTAC 2 cut(s) 145, 505
SaqAI TTAA 4 cut(s) 27, 387, 405, 462
SatI GCNGC 3 cut(s) 17, 160, 776
Sau3AI GATC 4 cut(s) 82, 429, 442, 517
Sau96I GGNCC 2 cut(s) 496, 748
SchI GAGTC 3 cut(s) 326, 564, 827
SduI GDGCHC 2 cut(s) 285, 600
SetI ASST 7 cut(s) 132, 146, 150, 526, 533, 710, 721
SfaNI GCATC 2 cut(s) 259, 474
Sfr274I CTCGAG 1 cut(s) 113
SinI GGWCC 2 cut(s) 496, 748
SlaI CTCGAG 1 cut(s) 113
SmlI CTYRAG 1 cut(s) 113
SmoI CTYRAG 1 cut(s) 113
SnaBI TACGTA 1 cut(s) 144
Sse9I AATT 6 cut(s) 174, 184, 380, 490, 753, 821
SsiI CCGC 1 cut(s) 235
SspMI CTAG 1 cut(s) 521
StyI CCWWGG 1 cut(s) 275
TaaI ACNGT 2 cut(s) 202, 500
TaiI ACGT 1 cut(s) 146
TaqI TCGA 1 cut(s) 114
TaqII GACCGA 1 cut(s) 765
TasI AATT 6 cut(s) 174, 184, 380, 490, 753, 821
TfiI GAWTC 1 cut(s) 227
Tru1I TTAA 4 cut(s) 27, 387, 405, 462
Tru9I TTAA 4 cut(s) 27, 387, 405, 462
TscAI CASTG 1 cut(s) 60
TseI GCWGC 3 cut(s) 16, 159, 775
TspDTI ATGAA 7 cut(s) 186, 240, 393, 675, 813, 834, 870
TspRI CASTG 1 cut(s) 60
Van91I CCANNNNNTGG 1 cut(s) 730
VpaK11BI GGWCC 2 cut(s) 496, 748
XapI RAATTY 3 cut(s) 380, 753, 821
XcmI CCANNNNNNNNNTGG 1 cut(s) 273
XhoI CTCGAG 1 cut(s) 113
XmnI GAANNNNTTC 1 cut(s) 131
XspI CTAG 1 cut(s) 521
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.