MD12G1022000.v1.1

Belongs to the eukaryotic ribosomal protein P1 P2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0001160
Physical Location & Seq
Reverse (-)
2188985 .. 2191026
2042 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1022000.v1.1.491

Sequence Viewer

Length: 345 bp
ATGAAGGTGGTCGCTGCATACTTGTTGGCTGTGTTGGGCGGCAAGACCACCCCTACCGCCGAAGATATCAAGGACATCCTTGGCTCCGTTGGTGCTGAGGCTGATGATGACAGGATTCAACTTCTGCTCTCCGAAGTCAAGGGTAAGGACATCACAGAGCTAATTGCCTCTGGAAGGGAAAAGTTGGCCTCTGTACCAGCTGGTGGAGGTGGTGCTGTTGCAGTGGCTGCAACTGGCAGTGGTGCTGCTGCTGCTGCCCCTGCTGCAGCTGAGGCGAAGAAAGAGGAGAAGGTTGAGGAGAAAGAGGAATCCGATGATGACATGGGATTCAGTCTCTTTGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_60s

Protein Analysis

115

Amino Acids

11.46

Weight (kDa)

4.4

Isoelectric Point (pI)

28.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_60s PF00428 18 - 113 2.7e-26 60s Acidic ribosomal protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 203
AciI CCGC 2 cut(s) 39, 57
AfaI GTAC 1 cut(s) 195
AfiI CCNNNNNNNGG 2 cut(s) 174, 203
AgsI TTSAA 1 cut(s) 119
AluBI AGCT 3 cut(s) 160, 200, 269
AluI AGCT 3 cut(s) 160, 200, 269
Alw26I GTCTC 1 cut(s) 338
AlwNI CAGNNNCTG 1 cut(s) 227
AoxI GGCC 1 cut(s) 186
ApeKI GCWGC 8 cut(s) 14, 227, 245, 248, 251, 254, 263, 266
BbvCI CCTCAGC 2 cut(s) 96, 270
BbvI GCAGC 7 cut(s) 214, 232, 235, 238, 241, 250, 278
BcoDI GTCTC 1 cut(s) 338
BfaI CTAG 1 cut(s) 343
BfmI CTRYAG 1 cut(s) 264
BisI GCNGC 9 cut(s) 15, 40, 228, 246, 249, 252, 255, 264, 267
BlsI GCNGC 9 cut(s) 16, 41, 229, 247, 250, 253, 256, 265, 268
BmiI GGNNCC 1 cut(s) 85
Bpu10I CCTNAGC 2 cut(s) 96, 270
BsaJI CCNNGG 1 cut(s) 79
Bsc4I CCNNNNNNNGG 2 cut(s) 174, 203
Bse1I ACTGG 1 cut(s) 238
BseDI CCNNGG 1 cut(s) 79
BseGI GGATG 1 cut(s) 75
BseLI CCNNNNNNNGG 2 cut(s) 174, 203
BseMII CTCAG 2 cut(s) 87, 261
BseNI ACTGG 1 cut(s) 238
BseRI GAGGAG 2 cut(s) 299, 311
BseXI GCAGC 7 cut(s) 214, 232, 235, 238, 241, 250, 278
BshFI GGCC 1 cut(s) 188
BslI CCNNNNNNNGG 2 cut(s) 174, 203
BsmAI GTCTC 1 cut(s) 338
BsnI GGCC 1 cut(s) 188
BspACI CCGC 2 cut(s) 39, 57
BspANI GGCC 1 cut(s) 188
BspCNI CTCAG 2 cut(s) 88, 262
BspLI GGNNCC 1 cut(s) 85
BspMAI CTGCAG 1 cut(s) 268
BsrI ACTGG 1 cut(s) 238
BssECI CCNNGG 1 cut(s) 79
BssT1I CCWWGG 1 cut(s) 79
BstAPI GCANNNNNTGC 1 cut(s) 227
BstDEI CTNAG 2 cut(s) 96, 270
BstENI CCTNNNNNAGG 1 cut(s) 172
BstF5I GGATG 1 cut(s) 75
BstMAI GTCTC 1 cut(s) 338
BstMWI GCNNNNNNNGC 6 cut(s) 227, 251, 254, 260, 263, 272
BstSFI CTRYAG 1 cut(s) 264
BstV1I GCAGC 7 cut(s) 214, 232, 235, 238, 241, 250, 278
BsuRI GGCC 1 cut(s) 188
BtsCI GGATG 1 cut(s) 75
BtsI GCAGTG 2 cut(s) 228, 244
BtsIMutI CAGTG 2 cut(s) 228, 244
CaiI CAGNNNCTG 1 cut(s) 227
Csp6I GTAC 1 cut(s) 194
CviAII CATG 1 cut(s) 322
CviJI RGCY 8 cut(s) 29, 84, 101, 160, 188, 200, 227, 269
CviKI_1 RGCY 8 cut(s) 29, 84, 101, 160, 188, 200, 227, 269
CviQI GTAC 1 cut(s) 194
DdeI CTNAG 2 cut(s) 96, 270
Eco130I CCWWGG 1 cut(s) 79
Eco32I GATATC 1 cut(s) 67
EcoNI CCTNNNNNAGG 1 cut(s) 172
EcoRV GATATC 1 cut(s) 67
EcoT14I CCWWGG 1 cut(s) 79
ErhI CCWWGG 1 cut(s) 79
FaeI CATG 1 cut(s) 325
FaiI YATR 2 cut(s) 19, 323
FatI CATG 1 cut(s) 321
Fnu4HI GCNGC 9 cut(s) 15, 40, 228, 246, 249, 252, 255, 264, 267
FokI GGATG 1 cut(s) 62
Fsp4HI GCNGC 9 cut(s) 15, 40, 228, 246, 249, 252, 255, 264, 267
FspBI CTAG 1 cut(s) 343
GluI GCNGC 9 cut(s) 15, 40, 228, 246, 249, 252, 255, 264, 267
HaeIII GGCC 1 cut(s) 188
Hin1II CATG 1 cut(s) 325
HinfI GANTC 3 cut(s) 115, 308, 327
Hpy188I TCNGA 2 cut(s) 133, 313
Hpy188III TCNNGA 1 cut(s) 171
HpyAV CCTTC 2 cut(s) 168, 283
HpyCH4V TGCA 4 cut(s) 17, 221, 230, 266
HpyF10VI GCNNNNNNNGC 6 cut(s) 227, 251, 254, 260, 263, 272
HpyF3I CTNAG 2 cut(s) 96, 270
Hsp92II CATG 1 cut(s) 325
LmnI GCTCC 1 cut(s) 89
LpnPI CCDG 6 cut(s) 97, 156, 186, 210, 219, 273
Lsp1109I GCAGC 7 cut(s) 214, 232, 235, 238, 241, 250, 278
MaeI CTAG 1 cut(s) 343
MboII GAAGA 2 cut(s) 74, 289
MluCI AATT 1 cut(s) 162
MnlI CCTC 8 cut(s) 91, 178, 199, 200, 265, 277, 289, 298
MspA1I CMGCKG 2 cut(s) 200, 269
MwoI GCNNNNNNNGC 6 cut(s) 227, 251, 254, 260, 263, 272
NlaIII CATG 1 cut(s) 325
NlaIV GGNNCC 1 cut(s) 85
PfeI GAWTC 3 cut(s) 115, 308, 327
PflMI CCANNNNNTGG 1 cut(s) 203
PkrI GCNGC 9 cut(s) 16, 41, 229, 247, 250, 253, 256, 265, 268
PspN4I GGNNCC 1 cut(s) 85
PstI CTGCAG 1 cut(s) 268
PstNI CAGNNNCTG 1 cut(s) 227
PvuII CAGCTG 2 cut(s) 200, 269
RsaI GTAC 1 cut(s) 195
RsaNI GTAC 1 cut(s) 194
SatI GCNGC 9 cut(s) 15, 40, 228, 246, 249, 252, 255, 264, 267
SetI ASST 6 cut(s) 9, 162, 202, 211, 271, 294
SfcI CTRYAG 1 cut(s) 264
Sse9I AATT 1 cut(s) 162
SsiI CCGC 2 cut(s) 39, 57
SspMI CTAG 1 cut(s) 343
StyI CCWWGG 1 cut(s) 79
TasI AATT 1 cut(s) 162
TauI GCSGC 1 cut(s) 42
TfiI GAWTC 3 cut(s) 115, 308, 327
TscAI CASTG 2 cut(s) 228, 244
TseI GCWGC 8 cut(s) 14, 227, 245, 248, 251, 254, 263, 266
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 76
TspRI CASTG 2 cut(s) 228, 244
Van91I CCANNNNNTGG 1 cut(s) 203
XagI CCTNNNNNAGG 1 cut(s) 172
XspI CTAG 1 cut(s) 343
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.