Rorug03G0126400

Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0001160
Physical Location & Seq
Forward (+)
10179429 .. 10182376
2948 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0126400.1

Sequence Viewer

Length: 855 bp
ATGATAATTGTAGCCCACACAATCCATGGAAACAAATGGGCTGCAATCGCAAGGCTTTTACCAGGAAGAACAGATAATGCCATAAAAAACCACTGGAACTCCACCCTACGTCGCCGGGATCCAGACAGAATGAAATCAGAATCAGGTAATATGGTTGAAGATGTTAGCTTGGACAAAACAATAGCATCTTCTGAAGAAACACTCTCAAATGGAGATGTCAATTCATCTAAATCTACGGAAGGAAAAGAAGTCAGTTCTCTAGAGTATACAGATGATCAACATGAAGACAAGGTGTCAACAGATGGTCTGTATAGTAATGAAGCCAGAGAACCTTGTACTCTTTTTAGGCCAGTGGCACGTGTAAGTGCTTTTAGTTTATATAACCATTTAGGTGGCCAGGATCCTTCATCATCATTGCAAAGGCCAATTCCAGTTCAAGGACCTCTGGTTCAAGTAGCAAAACTAGATGTAGGCATTTGTAAATTGGTCGAAGGAATTTATGGTGAGCGGTCGGTGCCTGATCAGTGTGGTCATGGCTGCTGTTGCACACAGAATGGCAGGAATACTGAAAACTCTTTGTTGGGGCCAGATTTTGTGGAATTCTCTGAGCCTCCCTGTTTTCCGAGTTTTGAATTGGCTGCAATTGCCACAGATATAAGTAACCTTGCTTGGCTTAAAAGTGGTTTGGAGAATAGTAGTGCAAGGGTAACAGGTGATGCAGCTGGTAGTATACTCTCTCATGCGTCTCAAGGGCAATTGGGGCATTTTGAGGAGAGTCGAATGAATCATCTTTTTCATCATGAAGAGAGAAAGGATAGGTTAATGAGCATGAAGACTAATGTGCTGTCAACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03050 map03050
Pfam Domains
Protein Families
Proteasome

Protein Analysis

284

Amino Acids

31.11

Weight (kDa)

5.56

Isoelectric Point (pI)

35.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 2 - 35 3e-11 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 514
AccBSI CCGCTC 1 cut(s) 508
AccI GTMKAC 2 cut(s) 266, 730
AciI CCGC 1 cut(s) 508
AclWI GGATC 4 cut(s) 113, 126, 395, 408
AcoI YGGCCR 1 cut(s) 394
AcsI RAATTY 2 cut(s) 495, 599
AcuI CTGAAG 1 cut(s) 213
AcvI CACGTG 1 cut(s) 359
AfaI GTAC 1 cut(s) 337
AfiI CCNNNNNNNGG 1 cut(s) 437
AflIII ACRYGT 1 cut(s) 358
AgsI TTSAA 4 cut(s) 158, 437, 452, 632
AhdI GACNNNNNGTC 1 cut(s) 292
AjnI CCWGG 2 cut(s) 61, 396
AloI GAACNNNNNNTCC 2 cut(s) 432, 464
AluBI AGCT 2 cut(s) 168, 722
AluI AGCT 2 cut(s) 168, 722
Alw26I GTCTC 1 cut(s) 750
AlwI GGATC 4 cut(s) 113, 126, 395, 408
AoxI GGCC 4 cut(s) 347, 394, 422, 584
ApeKI GCWGC 4 cut(s) 41, 537, 638, 719
ApoI RAATTY 2 cut(s) 495, 599
AspS9I GGNCC 2 cut(s) 440, 584
AsuC2I CCSGG 1 cut(s) 116
AsuHPI GGTGA 2 cut(s) 515, 725
AvaII GGWCC 1 cut(s) 440
BalI TGGCCA 1 cut(s) 396
BamHI GGATCC 2 cut(s) 118, 400
BanI GGYRCC 1 cut(s) 514
BbrPI CACGTG 1 cut(s) 359
BbsI GAAGAC 2 cut(s) 291, 839
BbvI GCAGC 4 cut(s) 28, 524, 625, 731
BccI CCATC 1 cut(s) 296
BciT130I CCWGG 2 cut(s) 63, 398
BclI TGATCA 2 cut(s) 274, 520
BcnI CCSGG 1 cut(s) 116
BcoDI GTCTC 1 cut(s) 750
BfaI CTAG 2 cut(s) 260, 464
BisI GCNGC 4 cut(s) 42, 538, 639, 720
BlsI GCNGC 4 cut(s) 43, 539, 640, 721
Bme1390I CCNGG 3 cut(s) 63, 116, 398
Bme18I GGWCC 1 cut(s) 440
BmeRI GACNNNNNGTC 1 cut(s) 292
BmgT120I GGNCC 2 cut(s) 440, 584
BmiI GGNNCC 4 cut(s) 120, 402, 516, 585
BmrFI CCNGG 3 cut(s) 63, 116, 398
BmsI GCATC 2 cut(s) 194, 706
BpiI GAAGAC 2 cut(s) 291, 839
BpuEI CTTGAG 1 cut(s) 732
BpuMI CCSGG 1 cut(s) 116
BsaAI YACGTR 1 cut(s) 359
BsaJI CCNNGG 1 cut(s) 25
BsaXI ACNNNNNCTCC 2 cut(s) 83, 113
Bsc4I CCNNNNNNNGG 1 cut(s) 437
Bse1I ACTGG 3 cut(s) 98, 350, 431
Bse3DI GCAATG 1 cut(s) 413
BseBI CCWGG 2 cut(s) 63, 398
BseDI CCNNGG 1 cut(s) 25
BseLI CCNNNNNNNGG 1 cut(s) 437
BseMI GCAATG 1 cut(s) 413
BseMII CTCAG 1 cut(s) 597
BseNI ACTGG 3 cut(s) 98, 350, 431
BseRI GAGGAG 1 cut(s) 785
BseXI GCAGC 4 cut(s) 28, 524, 625, 731
Bsh1285I CGRYCG 1 cut(s) 512
BshFI GGCC 4 cut(s) 349, 396, 424, 586
BshNI GGYRCC 1 cut(s) 514
BsiEI CGRYCG 1 cut(s) 512
BsiSI CCGG 1 cut(s) 115
BslI CCNNNNNNNGG 1 cut(s) 437
BsmAI GTCTC 1 cut(s) 750
BsmBI CGTCTC 1 cut(s) 750
BsnI GGCC 4 cut(s) 349, 396, 424, 586
Bsp143I GATC 4 cut(s) 118, 274, 400, 520
Bsp19I CCATGG 1 cut(s) 25
BspACI CCGC 1 cut(s) 508
BspANI GGCC 4 cut(s) 349, 396, 424, 586
BspCNI CTCAG 1 cut(s) 598
BspHI TCATGA 1 cut(s) 799
BspLI GGNNCC 4 cut(s) 120, 402, 516, 585
BspPI GGATC 4 cut(s) 113, 126, 395, 408
BspT107I GGYRCC 1 cut(s) 514
BsrBI CCGCTC 1 cut(s) 508
BsrDI GCAATG 1 cut(s) 413
BsrI ACTGG 3 cut(s) 98, 350, 431
BssECI CCNNGG 1 cut(s) 25
BssMI GATC 4 cut(s) 118, 274, 400, 520
BssNAI GTATAC 2 cut(s) 267, 731
BssT1I CCWWGG 1 cut(s) 25
Bst1107I GTATAC 2 cut(s) 267, 731
Bst2UI CCWGG 2 cut(s) 63, 398
Bst6I CTCTTC 1 cut(s) 798
BstBAI YACGTR 1 cut(s) 359
BstDEI CTNAG 1 cut(s) 606
BstDSI CCRYGG 1 cut(s) 25
BstKTI GATC 4 cut(s) 121, 277, 403, 523
BstMAI GTCTC 1 cut(s) 750
BstMBI GATC 4 cut(s) 118, 274, 400, 520
BstMCI CGRYCG 1 cut(s) 512
BstMWI GCNNNNNNNGC 5 cut(s) 47, 514, 543, 644, 760
BstNI CCWGG 2 cut(s) 63, 398
BstSCI CCNGG 3 cut(s) 61, 114, 396
BstV1I GCAGC 4 cut(s) 28, 524, 625, 731
BstV2I GAAGAC 2 cut(s) 291, 839
BstX2I RGATCY 2 cut(s) 118, 400
BstXI CCANNNNNNTGG 1 cut(s) 392
BstYI RGATCY 2 cut(s) 118, 400
BstZ17I GTATAC 2 cut(s) 267, 731
BsuRI GGCC 4 cut(s) 349, 396, 424, 586
BtgI CCRYGG 1 cut(s) 25
BtsIMutI CAGTG 3 cut(s) 91, 357, 530
CciI TCATGA 1 cut(s) 799
Cfr13I GGNCC 2 cut(s) 440, 584
CseI GACGC 1 cut(s) 732
Csp6I GTAC 1 cut(s) 336
CviAII CATG 6 cut(s) 26, 281, 533, 740, 800, 829
CviQI GTAC 1 cut(s) 336
DdeI CTNAG 1 cut(s) 606
DpnI GATC 4 cut(s) 120, 276, 402, 522
DpnII GATC 4 cut(s) 118, 274, 400, 520
DriI GACNNNNNGTC 1 cut(s) 292
EaeI YGGCCR 1 cut(s) 394
Eam1104I CTCTTC 1 cut(s) 798
Eam1105I GACNNNNNGTC 1 cut(s) 292
EarI CTCTTC 1 cut(s) 798
Eco130I CCWWGG 1 cut(s) 25
Eco47I GGWCC 1 cut(s) 440
Eco57I CTGAAG 1 cut(s) 213
Eco72I CACGTG 1 cut(s) 359
EcoO109I RGGNCCY 1 cut(s) 440
EcoRI GAATTC 1 cut(s) 599
EcoRII CCWGG 2 cut(s) 61, 396
EcoT14I CCWWGG 1 cut(s) 25
ErhI CCWWGG 1 cut(s) 25
Esp3I CGTCTC 1 cut(s) 750
FaeI CATG 6 cut(s) 29, 284, 536, 743, 803, 832
FatI CATG 6 cut(s) 25, 280, 532, 739, 799, 828
FbaI TGATCA 2 cut(s) 274, 520
FblI GTMKAC 2 cut(s) 266, 730
Fnu4HI GCNGC 4 cut(s) 42, 538, 639, 720
Fsp4HI GCNGC 4 cut(s) 42, 538, 639, 720
FspBI CTAG 2 cut(s) 260, 464
GluI GCNGC 4 cut(s) 42, 538, 639, 720
HaeIII GGCC 4 cut(s) 349, 396, 424, 586
HapII CCGG 1 cut(s) 115
HgaI GACGC 1 cut(s) 732
Hin1II CATG 6 cut(s) 29, 284, 536, 743, 803, 832
HincII GTYRAC 2 cut(s) 297, 849
HindII GTYRAC 2 cut(s) 297, 849
HinfI GANTC 3 cut(s) 140, 775, 784
HpaII CCGG 1 cut(s) 115
HphI GGTGA 2 cut(s) 515, 725
Hpy166II GTNNAC 4 cut(s) 267, 297, 731, 849
Hpy188I TCNGA 4 cut(s) 139, 193, 607, 624
Hpy188III TCNNGA 3 cut(s) 122, 260, 800
Hpy8I GTNNAC 4 cut(s) 267, 297, 731, 849
Hpy99I CGWCG 1 cut(s) 114
HpyAV CCTTC 3 cut(s) 233, 414, 485
HpyCH4IV ACGT 2 cut(s) 109, 358
HpyCH4V TGCA 6 cut(s) 44, 418, 546, 641, 701, 719
HpyF10VI GCNNNNNNNGC 5 cut(s) 47, 514, 543, 644, 760
HpyF3I CTNAG 1 cut(s) 606
HpySE526I ACGT 2 cut(s) 109, 358
Hsp92II CATG 6 cut(s) 29, 284, 536, 743, 803, 832
Ksp22I TGATCA 2 cut(s) 274, 520
Kzo9I GATC 4 cut(s) 118, 274, 400, 520
Lsp1109I GCAGC 4 cut(s) 28, 524, 625, 731
LweI GCATC 2 cut(s) 194, 706
MaeI CTAG 2 cut(s) 260, 464
MaeII ACGT 2 cut(s) 109, 358
MaeIII GTNAC 2 cut(s) 659, 706
MalI GATC 4 cut(s) 120, 276, 402, 522
MbiI CCGCTC 1 cut(s) 508
MboI GATC 4 cut(s) 118, 274, 400, 520
MboII GAAGA 7 cut(s) 78, 170, 180, 206, 296, 815, 844
MfeI CAATTG 2 cut(s) 642, 755
MflI RGATCY 2 cut(s) 118, 400
MlsI TGGCCA 1 cut(s) 396
MluCI AATT 9 cut(s) 6, 220, 426, 482, 495, 599, 632, 642, 755
MluNI TGGCCA 1 cut(s) 396
MlyI GAGTC 1 cut(s) 784
MnlI CCTC 3 cut(s) 453, 621, 763
Mox20I TGGCCA 1 cut(s) 396
MscI TGGCCA 1 cut(s) 396
MseI TTAA 2 cut(s) 675, 821
MslI CAYNNNNRTG 1 cut(s) 390
Msp20I TGGCCA 1 cut(s) 396
MspA1I CMGCKG 1 cut(s) 722
MspI CCGG 1 cut(s) 115
MspR9I CCNGG 3 cut(s) 63, 116, 398
MunI CAATTG 2 cut(s) 642, 755
MvaI CCWGG 2 cut(s) 63, 398
MwoI GCNNNNNNNGC 5 cut(s) 47, 514, 543, 644, 760
NciI CCSGG 1 cut(s) 116
NcoI CCATGG 1 cut(s) 25
NdeII GATC 4 cut(s) 118, 274, 400, 520
NlaIII CATG 6 cut(s) 29, 284, 536, 743, 803, 832
NlaIV GGNNCC 4 cut(s) 120, 402, 516, 585
PagI TCATGA 1 cut(s) 799
PfeI GAWTC 2 cut(s) 140, 784
PkrI GCNGC 4 cut(s) 43, 539, 640, 721
PleI GAGTC 1 cut(s) 783
PmaCI CACGTG 1 cut(s) 359
PmlI CACGTG 1 cut(s) 359
PpsI GAGTC 1 cut(s) 783
Ppu21I YACGTR 1 cut(s) 359
PpuMI RGGWCCY 1 cut(s) 440
Psp5II RGGWCCY 1 cut(s) 440
Psp6I CCWGG 2 cut(s) 61, 396
PspCI CACGTG 1 cut(s) 359
PspGI CCWGG 2 cut(s) 61, 396
PspN4I GGNNCC 4 cut(s) 120, 402, 516, 585
PspPI GGNCC 2 cut(s) 440, 584
PspPPI RGGWCCY 1 cut(s) 440
PsuI RGATCY 2 cut(s) 118, 400
PvuII CAGCTG 1 cut(s) 722
RsaI GTAC 1 cut(s) 337
RsaNI GTAC 1 cut(s) 336
RseI CAYNNNNRTG 1 cut(s) 390
SaqAI TTAA 2 cut(s) 675, 821
SatI GCNGC 4 cut(s) 42, 538, 639, 720
Sau3AI GATC 4 cut(s) 118, 274, 400, 520
Sau96I GGNCC 2 cut(s) 440, 584
SchI GAGTC 1 cut(s) 784
ScrFI CCNGG 3 cut(s) 63, 116, 398
SfaNI GCATC 2 cut(s) 194, 706
SinI GGWCC 1 cut(s) 440
SmiMI CAYNNNNRTG 1 cut(s) 390
SmlI CTYRAG 1 cut(s) 747
SmoI CTYRAG 1 cut(s) 747
Sse9I AATT 9 cut(s) 6, 220, 426, 482, 495, 599, 632, 642, 755
SsiI CCGC 1 cut(s) 508
SspMI CTAG 2 cut(s) 260, 464
StyD4I CCNGG 3 cut(s) 61, 114, 396
StyI CCWWGG 1 cut(s) 25
TaiI ACGT 2 cut(s) 112, 361
TaqI TCGA 2 cut(s) 489, 778
TasI AATT 9 cut(s) 6, 220, 426, 482, 495, 599, 632, 642, 755
TatI WGTACW 1 cut(s) 335
TfiI GAWTC 2 cut(s) 140, 784
Tru1I TTAA 2 cut(s) 675, 821
Tru9I TTAA 2 cut(s) 675, 821
TscAI CASTG 3 cut(s) 98, 357, 530
TseI GCWGC 4 cut(s) 41, 537, 638, 719
TspDTI ATGAA 9 cut(s) 146, 213, 297, 333, 396, 785, 797, 816, 845
TspGWI ACGGA 1 cut(s) 251
TspRI CASTG 3 cut(s) 98, 357, 530
VpaK11BI GGWCC 1 cut(s) 440
XapI RAATTY 2 cut(s) 495, 599
XbaI TCTAGA 1 cut(s) 259
XcmI CCANNNNNNNNNTGG 1 cut(s) 23
XmiI GTMKAC 2 cut(s) 266, 730
XspI CTAG 2 cut(s) 260, 464
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.