MD12G1120800.v1.1

May be involved in modulation of pathogen defense and leaf cell death

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0000744
Physical Location & Seq
Reverse (-)
19368007 .. 19372570
4564 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1120800.v1.1.491

Sequence Viewer

Length: 1737 bp
ATGGCAGGAGGAAAAAAAGGAAGATCTTTGGAGCAAACACCAACTTGGGCAGTTGCCGTTGTTTGTTTCGTTTTGGTTTTAATTTCAATCCTCATTGAATATTTCATTCATTTAATTGGAAAGTGGTTGAAAAAGAGAAACAAAAGAGCTCTCTATGAAGCACTTGAGAAGATCAAGTCGGAGCTTATGCTATTAGGGTTCCTATCCTTGCTTCTAACAGTAGGACAAGGCCCCATTTCAAATATTTGTATATCGAAGGCTGTTGGAGCAACTTGGCATCCATGCAGTAAGAAGCAAGAGGTCAAATCAGACAAGAACGAAGATAAGAGTAGTGTTTCAGATGACAACGCCCGCAGAAGGCTTCTCTCAGCCTTGGATTCCAGTGGGGGTGGCCGACGTGTTTTAGCAGCTGCTGGATATGACAAATGTGCTGCCAAGAACAAAGTTCCATTTGTGTCATATTATGGGATCCACCAGCTTCACATACTCATCTTTGTGCTAGCTGTCTTCCACGTGCTTTACTGCATAACAACCCTAGTTTTGGGCAGAGCAAAGATGAGAAAATGGAAAACATGGGAACTGGAAACAAAAACAGCTGCCTACCAATTCTCACATGACCCGGAAAGATTCAGATTTGCCAGAGACACATCCTTTGGGAGAAGACACTTAAGCTTTTGGAGCCGTTCTCCCATTAGCTTATGGATTGTTTGCTTCTTCAGACAGTTTGTAAGATCTGTGCCCAAAGTTGATTACTTGACTCTGCGACATGGGTTTATTGCAGCACATTTGGCTCCTCAAAGTCAAACTAAGTTTGACTTCCAGAAGTACATTAACAGGTCACTTGAAGAGGATTTCAAGGTTGTCGTAGGGATCAGCCCAACTATTTGGTTATTTGCTGTATTAATCCTCATGTCAAACACCCACGGCTCACGCTCTTATCTGTGGCTCCCATTTGTTCCCCTAGTTATGATCTTAATGGTAGGGACAAAGCTGCAAGTTATAATAACAAAGATGGGGCTCAAATTATCAGAAAGAGGTGAAGTGGTTAGGGGAACCCCACTGGTTGAGCCTGGTGACCATCTCTTCTGGTTCAACAACCCCCGACTCCTGCTTTATATCATCCACTTTGTTCTCTTTCAGAATGCTTTTGCACTTGCTTTCTTTGCATGGACTTGGTATGAATTTGGCTTGAAGTCTTGCTTCCATGAGAAGTTGGAAGATGTCGTTTTAAGAATATCAATGGGGGTGATCATACAAATTCTGTGCAGCTATGTAACTCTTCCTCTTTATGCATTGGTAACACAGATGGGTTCAACTATGAAGCCTGTAATCTTCAACGACAGAGTGGCGACTGCACTCAAAAAATGGCACATCGCAGCGAAGAAGCACGTAAAACACAAGAATGCAAGTCCAGCCAGTGCACCAGGCACTCCTTTGCACTCCATGTCCCCTGTTCATCTACTACGCAACTATAAATATGAACAAGACATTGACAGCATTCAGACATCACCAAGAATGCCTTATTTTGATAACGAAGGTTCAGACTCACCGTTTCATCACCAAGACAATTTGACTTGGTCTCAGCAAGGTACAAACATGGAGGGTCAGAAGGAGGAGATTAGTGCTCATGGACCTAACGCAGAGAGCAATGCTTTAGGCGCTTATGGTTCGATAATTCAACATGAGATTCAAATTCACTCGGCGGCACTCACATTTGAGAAAACAGAAAGAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mlo

Protein Analysis

579

Amino Acids

65.72

Weight (kDa)

9.57

Isoelectric Point (pI)

46.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mlo PF03094 7 - 492 2.9e-238 Mlo family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1001
AciI CCGC 2 cut(s) 352, 1703
AclWI GGATC 3 cut(s) 463, 476, 878
AcoI YGGCCR 1 cut(s) 391
AcsI RAATTY 3 cut(s) 1181, 1257, 1692
AcuI CTGAAG 1 cut(s) 700
AcvI CACGTG 1 cut(s) 514
AfaI GTAC 2 cut(s) 827, 1591
AfiI CCNNNNNNNGG 2 cut(s) 357, 541
AflII CTTAAG 1 cut(s) 667
AflIII ACRYGT 1 cut(s) 397
AjiI CACGTC 1 cut(s) 398
AjnI CCWGG 2 cut(s) 1069, 1423
Alw21I GWGCWC 3 cut(s) 151, 1423, 1627
Alw26I GTCTC 2 cut(s) 636, 1584
Alw44I GTGCAC 1 cut(s) 1419
AlwI GGATC 3 cut(s) 463, 476, 878
AlwNI CAGNNNCTG 1 cut(s) 413
AoxI GGCC 2 cut(s) 229, 391
ApaLI GTGCAC 1 cut(s) 1419
ApeKI GCWGC 8 cut(s) 407, 410, 431, 596, 779, 991, 1266, 1376
ApoI RAATTY 3 cut(s) 1181, 1257, 1692
ArsI GACNNNNNNTTYG 2 cut(s) 635, 667
AseI ATTAAT 1 cut(s) 902
AspLEI GCGC 1 cut(s) 1661
AspS9I GGNCC 2 cut(s) 230, 1631
AsuC2I CCSGG 1 cut(s) 620
AsuHPI GGTGA 6 cut(s) 1049, 1085, 1258, 1500, 1539, 1550
AsuNHI GCTAGC 1 cut(s) 499
AvaII GGWCC 1 cut(s) 1631
BaeGI GKGCMC 2 cut(s) 741, 1423
BamHI GGATCC 1 cut(s) 468
BanII GRGCYC 2 cut(s) 151, 1020
BbrPI CACGTG 1 cut(s) 514
BbsI GAAGAC 2 cut(s) 499, 667
Bbv12I GWGCWC 3 cut(s) 151, 1423, 1627
BbvI GCAGC 8 cut(s) 397, 418, 419, 583, 791, 978, 1278, 1388
BccI CCATC 3 cut(s) 1006, 1086, 1300
BceAI ACGGC 3 cut(s) 41, 666, 940
BciT130I CCWGG 2 cut(s) 1071, 1425
BclI TGATCA 1 cut(s) 1248
BcnI CCSGG 1 cut(s) 620
BcoDI GTCTC 2 cut(s) 636, 1584
BfaI CTAG 3 cut(s) 500, 536, 962
BfoI RGCGCY 1 cut(s) 1662
BfrI CTTAAG 1 cut(s) 667
BglII AGATCT 2 cut(s) 23, 731
BisI GCNGC 9 cut(s) 408, 411, 432, 597, 780, 992, 1267, 1377, 1704
BlsI GCNGC 9 cut(s) 409, 412, 433, 598, 781, 993, 1268, 1378, 1705
Bme1390I CCNGG 3 cut(s) 620, 1071, 1425
Bme18I GGWCC 1 cut(s) 1631
BmgBI CACGTC 1 cut(s) 398
BmgT120I GGNCC 2 cut(s) 230, 1631
BmiI GGNNCC 7 cut(s) 200, 232, 470, 680, 792, 947, 1054
BmrFI CCNGG 3 cut(s) 620, 1071, 1425
BmsI GCATC 1 cut(s) 286
BmtI GCTAGC 1 cut(s) 503
BpiI GAAGAC 2 cut(s) 499, 667
BplI GAGNNNNNCTC 2 cut(s) 670, 702
BpuEI CTTGAG 1 cut(s) 185
BpuMI CCSGG 1 cut(s) 620
BsaAI YACGTR 2 cut(s) 514, 1390
BsaI GGTCTC 1 cut(s) 1584
BsaJI CCNNGG 2 cut(s) 372, 922
Bsc4I CCNNNNNNNGG 2 cut(s) 357, 541
Bse1I ACTGG 4 cut(s) 381, 585, 1065, 1416
Bse3DI GCAATG 1 cut(s) 1654
BseBI CCWGG 2 cut(s) 1071, 1425
BseDI CCNNGG 2 cut(s) 372, 922
BseGI GGATG 3 cut(s) 277, 647, 1119
BseLI CCNNNNNNNGG 2 cut(s) 357, 541
BseMI GCAATG 1 cut(s) 1654
BseMII CTCAG 2 cut(s) 381, 1595
BseNI ACTGG 4 cut(s) 381, 585, 1065, 1416
BseRI GAGGAG 2 cut(s) 783, 1628
BseSI GKGCMC 2 cut(s) 741, 1423
BseXI GCAGC 8 cut(s) 397, 418, 419, 583, 791, 978, 1278, 1388
BsgI GTGCAG 2 cut(s) 1285, 1338
BshFI GGCC 2 cut(s) 231, 393
BsiHKAI GWGCWC 3 cut(s) 151, 1423, 1627
BsiSI CCGG 1 cut(s) 620
BslFI GGGAC 2 cut(s) 997, 1432
BslI CCNNNNNNNGG 2 cut(s) 357, 541
BsmAI GTCTC 2 cut(s) 636, 1584
BsmFI GGGAC 2 cut(s) 997, 1432
BsmI GAATGC 4 cut(s) 1147, 1408, 1497, 1521
BsnI GGCC 2 cut(s) 231, 393
Bso31I GGTCTC 1 cut(s) 1584
Bsp1286I GDGCHC 5 cut(s) 151, 741, 1020, 1423, 1627
Bsp143I GATC 7 cut(s) 23, 171, 468, 731, 870, 969, 1248
BspACI CCGC 2 cut(s) 352, 1703
BspANI GGCC 2 cut(s) 231, 393
BspCNI CTCAG 2 cut(s) 380, 1594
BspLI GGNNCC 7 cut(s) 200, 232, 470, 680, 792, 947, 1054
BspOI GCTAGC 1 cut(s) 503
BspPI GGATC 3 cut(s) 463, 476, 878
BspTI CTTAAG 1 cut(s) 667
BspTNI GGTCTC 1 cut(s) 1584
BsrDI GCAATG 1 cut(s) 1654
BsrI ACTGG 4 cut(s) 381, 585, 1065, 1416
BssECI CCNNGG 2 cut(s) 372, 922
BssMI GATC 7 cut(s) 23, 171, 468, 731, 870, 969, 1248
BssT1I CCWWGG 1 cut(s) 372
Bst2UI CCWGG 2 cut(s) 1071, 1425
Bst4CI ACNGT 3 cut(s) 220, 723, 1551
Bst6I CTCTTC 3 cut(s) 840, 1088, 1284
BstAFI CTTAAG 1 cut(s) 667
BstBAI YACGTR 2 cut(s) 514, 1390
BstC8I GCNNGC 2 cut(s) 352, 501
BstDEI CTNAG 3 cut(s) 367, 807, 1581
BstDSI CCRYGG 1 cut(s) 922
BstEII GGTNACC 1 cut(s) 1073
BstF5I GGATG 3 cut(s) 277, 647, 1119
BstH2I RGCGCY 1 cut(s) 1662
BstHHI GCGC 1 cut(s) 1661
BstKTI GATC 7 cut(s) 26, 174, 471, 734, 873, 972, 1251
BstMAI GTCTC 2 cut(s) 636, 1584
BstMBI GATC 7 cut(s) 23, 171, 468, 731, 870, 969, 1248
BstMWI GCNNNNNNNGC 6 cut(s) 266, 678, 788, 1163, 1412, 1658
BstNI CCWGG 2 cut(s) 1071, 1425
BstPI GGTNACC 1 cut(s) 1073
BstSCI CCNGG 3 cut(s) 618, 1069, 1423
BstSLI GKGCMC 2 cut(s) 741, 1423
BstV1I GCAGC 8 cut(s) 397, 418, 419, 583, 791, 978, 1278, 1388
BstV2I GAAGAC 2 cut(s) 499, 667
BstX2I RGATCY 3 cut(s) 23, 468, 731
BstXI CCANNNNNNTGG 1 cut(s) 885
BstYI RGATCY 3 cut(s) 23, 468, 731
BsuRI GGCC 2 cut(s) 231, 393
BtgI CCRYGG 1 cut(s) 922
BtgZI GCGATG 1 cut(s) 1357
BtrI CACGTC 1 cut(s) 398
BtsCI GGATG 3 cut(s) 277, 647, 1119
BtsIMutI CAGTG 3 cut(s) 388, 1058, 1423
Cac8I GCNNGC 2 cut(s) 352, 501
CaiI CAGNNNCTG 1 cut(s) 413
CfoI GCGC 1 cut(s) 1661
Cfr13I GGNCC 2 cut(s) 230, 1631
Csp6I GTAC 2 cut(s) 826, 1590
CviQI GTAC 2 cut(s) 826, 1590
DdeI CTNAG 3 cut(s) 367, 807, 1581
DpnI GATC 7 cut(s) 25, 173, 470, 733, 872, 971, 1250
DpnII GATC 7 cut(s) 23, 171, 468, 731, 870, 969, 1248
EaeI YGGCCR 1 cut(s) 391
Eam1104I CTCTTC 3 cut(s) 840, 1088, 1284
EarI CTCTTC 3 cut(s) 840, 1088, 1284
Ecl136II GAGCTC 1 cut(s) 149
Eco130I CCWWGG 1 cut(s) 372
Eco24I GRGCYC 2 cut(s) 151, 1020
Eco31I GGTCTC 1 cut(s) 1584
Eco47I GGWCC 1 cut(s) 1631
Eco53kI GAGCTC 1 cut(s) 149
Eco57I CTGAAG 1 cut(s) 700
Eco72I CACGTG 1 cut(s) 514
Eco91I GGTNACC 1 cut(s) 1073
EcoICRI GAGCTC 1 cut(s) 149
EcoO109I RGGNCCY 1 cut(s) 230
EcoO65I GGTNACC 1 cut(s) 1073
EcoRII CCWGG 2 cut(s) 1069, 1423
EcoT14I CCWWGG 1 cut(s) 372
EcoT22I ATGCAT 1 cut(s) 1294
EcoT38I GRGCYC 2 cut(s) 151, 1020
ErhI CCWWGG 1 cut(s) 372
FalI AAGNNNNNCTT 6 cut(s) 800, 832, 1184, 1216, 1504, 1536
FaqI GGGAC 2 cut(s) 997, 1432
FauI CCCGC 1 cut(s) 359
FbaI TGATCA 1 cut(s) 1248
Fnu4HI GCNGC 9 cut(s) 408, 411, 432, 597, 780, 992, 1267, 1377, 1704
FokI GGATG 3 cut(s) 264, 634, 1106
FriOI GRGCYC 2 cut(s) 151, 1020
Fsp4HI GCNGC 9 cut(s) 408, 411, 432, 597, 780, 992, 1267, 1377, 1704
FspBI CTAG 3 cut(s) 500, 536, 962
GlaI GCGC 1 cut(s) 1660
GluI GCNGC 9 cut(s) 408, 411, 432, 597, 780, 992, 1267, 1377, 1704
HaeII RGCGCY 1 cut(s) 1662
HaeIII GGCC 2 cut(s) 231, 393
HapII CCGG 1 cut(s) 620
HhaI GCGC 1 cut(s) 1661
Hin6I GCGC 1 cut(s) 1659
HinP1I GCGC 1 cut(s) 1659
HindIII AAGCTT 1 cut(s) 670
HinfI GANTC 6 cut(s) 377, 627, 757, 1104, 1544, 1687
HpaII CCGG 1 cut(s) 620
HphI GGTGA 6 cut(s) 1049, 1085, 1258, 1500, 1539, 1550
Hpy166II GTNNAC 1 cut(s) 1421
Hpy188III TCNNGA 1 cut(s) 820
Hpy8I GTNNAC 1 cut(s) 1421
Hpy99I CGWCG 1 cut(s) 399
HpyAV CCTTC 4 cut(s) 250, 351, 1529, 1603
HpyCH4III ACNGT 3 cut(s) 220, 723, 1551
HpyCH4IV ACGT 3 cut(s) 397, 513, 1389
HpyF10VI GCNNNNNNNGC 6 cut(s) 266, 678, 788, 1163, 1412, 1658
HpyF3I CTNAG 3 cut(s) 367, 807, 1581
HpySE526I ACGT 3 cut(s) 397, 513, 1389
HspAI GCGC 1 cut(s) 1659
Ksp22I TGATCA 1 cut(s) 1248
Kzo9I GATC 7 cut(s) 23, 171, 468, 731, 870, 969, 1248
LmnI GCTCC 6 cut(s) 31, 181, 266, 678, 796, 951
Lsp1109I GCAGC 8 cut(s) 397, 418, 419, 583, 791, 978, 1278, 1388
LweI GCATC 1 cut(s) 286
MaeI CTAG 3 cut(s) 500, 536, 962
MaeII ACGT 3 cut(s) 397, 513, 1389
MaeIII GTNAC 4 cut(s) 837, 1073, 1273, 1297
MalI GATC 7 cut(s) 25, 173, 470, 733, 872, 971, 1250
MboI GATC 7 cut(s) 23, 171, 468, 731, 870, 969, 1248
MflI RGATCY 3 cut(s) 23, 468, 731
MhlI GDGCHC 5 cut(s) 151, 741, 1020, 1423, 1627
MluCI AATT 9 cut(s) 81, 114, 605, 1022, 1181, 1257, 1567, 1674, 1692
MlyI GAGTC 3 cut(s) 751, 1098, 1538
MmeI TCCRAC 3 cut(s) 159, 244, 1194
MnlI CCTC 9 cut(s) 101, 292, 804, 841, 917, 1028, 1293, 1594, 1606
Mph1103I ATGCAT 1 cut(s) 1294
MseI TTAA 7 cut(s) 80, 113, 668, 831, 902, 974, 1229
MslI CAYNNNNRTG 2 cut(s) 494, 1401
MspA1I CMGCKG 2 cut(s) 410, 596
MspCI CTTAAG 1 cut(s) 667
MspI CCGG 1 cut(s) 620
MspR9I CCNGG 3 cut(s) 620, 1071, 1425
Mva1269I GAATGC 4 cut(s) 1147, 1408, 1497, 1521
MvaI CCWGG 2 cut(s) 1071, 1425
MwoI GCNNNNNNNGC 6 cut(s) 266, 678, 788, 1163, 1412, 1658
NciI CCSGG 1 cut(s) 620
NdeII GATC 7 cut(s) 23, 171, 468, 731, 870, 969, 1248
NheI GCTAGC 1 cut(s) 499
NlaIV GGNNCC 7 cut(s) 200, 232, 470, 680, 792, 947, 1054
NmeAIII GCCGAG 1 cut(s) 1679
NmuCI GTSAC 2 cut(s) 837, 1073
NsiI ATGCAT 1 cut(s) 1294
PctI GAATGC 4 cut(s) 1147, 1408, 1497, 1521
PfeI GAWTC 3 cut(s) 377, 627, 1687
PflFI GACNNNGTC 1 cut(s) 1576
PkrI GCNGC 9 cut(s) 409, 412, 433, 598, 781, 993, 1268, 1378, 1705
PleI GAGTC 3 cut(s) 751, 1098, 1538
PmaCI CACGTG 1 cut(s) 514
PmlI CACGTG 1 cut(s) 514
PpsI GAGTC 3 cut(s) 751, 1098, 1538
Ppu21I YACGTR 2 cut(s) 514, 1390
PshBI ATTAAT 1 cut(s) 902
PsiI TTATAA 1 cut(s) 1001
Psp124BI GAGCTC 1 cut(s) 151
Psp6I CCWGG 2 cut(s) 1069, 1423
PspCI CACGTG 1 cut(s) 514
PspEI GGTNACC 1 cut(s) 1073
PspGI CCWGG 2 cut(s) 1069, 1423
PspN4I GGNNCC 7 cut(s) 200, 232, 470, 680, 792, 947, 1054
PspPI GGNCC 2 cut(s) 230, 1631
PstNI CAGNNNCTG 1 cut(s) 413
PsuI RGATCY 3 cut(s) 23, 468, 731
PsyI GACNNNGTC 1 cut(s) 1576
PvuII CAGCTG 2 cut(s) 410, 596
RsaI GTAC 2 cut(s) 827, 1591
RsaNI GTAC 2 cut(s) 826, 1590
RseI CAYNNNNRTG 2 cut(s) 494, 1401
SacI GAGCTC 1 cut(s) 151
SaqAI TTAA 7 cut(s) 80, 113, 668, 831, 902, 974, 1229
SatI GCNGC 9 cut(s) 408, 411, 432, 597, 780, 992, 1267, 1377, 1704
Sau3AI GATC 7 cut(s) 23, 171, 468, 731, 870, 969, 1248
Sau96I GGNCC 2 cut(s) 230, 1631
SchI GAGTC 3 cut(s) 751, 1098, 1538
ScrFI CCNGG 3 cut(s) 620, 1071, 1425
SduI GDGCHC 5 cut(s) 151, 741, 1020, 1423, 1627
SfaNI GCATC 1 cut(s) 286
SinI GGWCC 1 cut(s) 1631
SmiMI CAYNNNNRTG 2 cut(s) 494, 1401
SmlI CTYRAG 2 cut(s) 164, 667
SmoI CTYRAG 2 cut(s) 164, 667
Sse9I AATT 9 cut(s) 81, 114, 605, 1022, 1181, 1257, 1567, 1674, 1692
SsiI CCGC 2 cut(s) 352, 1703
SspI AATATT 2 cut(s) 101, 244
SspMI CTAG 3 cut(s) 500, 536, 962
SstI GAGCTC 1 cut(s) 151
StyD4I CCNGG 3 cut(s) 618, 1069, 1423
StyI CCWWGG 1 cut(s) 372
TaaI ACNGT 3 cut(s) 220, 723, 1551
TaiI ACGT 3 cut(s) 400, 516, 1392
TaqI TCGA 2 cut(s) 254, 1670
TasI AATT 9 cut(s) 81, 114, 605, 1022, 1181, 1257, 1567, 1674, 1692
TatI WGTACW 1 cut(s) 825
TauI GCSGC 1 cut(s) 1706
TfiI GAWTC 3 cut(s) 377, 627, 1687
Tru1I TTAA 7 cut(s) 80, 113, 668, 831, 902, 974, 1229
Tru9I TTAA 7 cut(s) 80, 113, 668, 831, 902, 974, 1229
TscAI CASTG 3 cut(s) 388, 1065, 1423
TseFI GTSAC 2 cut(s) 837, 1073
TseI GCWGC 8 cut(s) 407, 410, 431, 596, 779, 991, 1266, 1376
Tsp45I GTSAC 2 cut(s) 837, 1073
TspDTI ATGAA 8 cut(s) 94, 98, 171, 1194, 1334, 1445, 1494, 1544
TspRI CASTG 3 cut(s) 388, 1065, 1423
Tth111I GACNNNGTC 1 cut(s) 1576
Vha464I CTTAAG 1 cut(s) 667
VneI GTGCAC 1 cut(s) 1419
VpaK11BI GGWCC 1 cut(s) 1631
VspI ATTAAT 1 cut(s) 902
XapI RAATTY 3 cut(s) 1181, 1257, 1692
XspI CTAG 3 cut(s) 500, 536, 962
Zsp2I ATGCAT 1 cut(s) 1294
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.