Rh3BG172700

May be involved in modulation of pathogen defense and leaf cell death

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0000744
Physical Location & Seq
Forward (+)
14802515 .. 14807243
4729 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG172700.1

Sequence Viewer

Length: 1215 bp
ATGGCAACAGCTACAAAGGAACGATCTTTGGAGCAAACGCCGACGTGGGCCGTTGCCGTTGTTTGTTTCTTTTTGGTTTTGGTCTCCATCATCATCGAGCATATCCTTCATTTGATCGGAAAGTGGTTGGAAAGGAAGAATAAACGAGCTCTCTGTGAAGCACTGGAAAAAATCAAAGCAGAGCTTATGTTATTGGGGTTCCTCTCGCTGCTGCTAACAGTAGGACAAGGGCCCATTTCGGACATTTGCATATCAAAGTCTGTTGGGGCCACTTGGCATCCATGTAGTAAGCAGCAAGAGACAGTCACTGCAGAGGAGGATGGAGAGAGCACTAGTCCAGAATCAGATGATGACAGCGGCCGTAGAAGGCTACTATCGGCGGTTCTCTCGAGTTCCGGTGAGAGTGCCCGACGTGTTTTGGCCGCAGCCGGATATGACAAATGCGGTGCCCAGGGAAAAGTTCCGTTTGTGTCATTTTATGGGATTCACCAGCTTCATATACTCATCTTCGTGCTAGCCGTTTCCCACGTGCTTTACTGCATAATAACACTGGTTTTGGGCCGAGCAAAGATGAGGAGATGGAAAAAATGGGAATTGGAAACCAGGACAGTAGACTACCAGTTCTCACACGACCCAGAAAGATTCAGATTTGCCAGAGACACATCGTTTGGGCGAAGGCACTTGAGCTTTTGGAGCCAGTCAACCATTTTGTTATGGATTGTTTGCTTCTTCAGACAGTTTGTCAGATCAGTGCCTAAGGTCGATTACCTGACGCTACGCAACGGGTTTATCTTTGCACATTTGGCACCTCAGAGTCAAACCAAGTTTGATTTCCAGAAGTACATTAACAGATCACTTGAAGAGGATTTCAAGGTCGTGGTGGGGATCAGCCCTCCGATCTGGTTATTCGCTGTATTGTTCCTGCTATCTAACACCCACGGCTCACGTTCATATCTCTATCTACCCTTTATTCCACTAGTCATGATCTTATTAGTAGGGACAAAGCTACAGGTTATAATAACCAAGATGGGACTTAAAATTCAAGAGAGGGGAGAAGTGGTTAAGGGGACCCCGCTTGTGGAGCCTGGTGACCATCTCTTCTGGTTCAACAACCCACGTCTTATTCTTCACATCATCCATTTTGTTCTCTTTCAGGTTCGAATATATTCATCCCAACTATACAGTACACTGGAAATTATTCTCATCTCCGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mlo

Protein Analysis

404

Amino Acids

46.1

Weight (kDa)

9.31

Isoelectric Point (pI)

47.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mlo PF03094 7 - 385 3.3e-184 Mlo family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1016
AccB1I GGYRCC 2 cut(s) 446, 805
AccI GTMKAC 1 cut(s) 612
AciI CCGC 5 cut(s) 357, 380, 423, 444, 1073
AclWI GGATC 1 cut(s) 893
AcoI YGGCCR 2 cut(s) 358, 420
AcsI RAATTY 1 cut(s) 1038
AcuI CTGAAG 1 cut(s) 715
AcvI CACGTG 1 cut(s) 529
AfaI GTAC 2 cut(s) 842, 1186
AfiI CCNNNNNNNGG 1 cut(s) 1078
AflIII ACRYGT 1 cut(s) 412
AgsI TTSAA 4 cut(s) 860, 871, 1043, 1108
AhdI GACNNNNNGTC 1 cut(s) 740
AhlI ACTAGT 2 cut(s) 332, 976
AjiI CACGTC 3 cut(s) 45, 413, 1118
AjnI CCWGG 3 cut(s) 450, 602, 1084
AjuI GAANNNNNNNTTGG 2 cut(s) 815, 847
AluBI AGCT 6 cut(s) 11, 149, 184, 493, 687, 1006
AluI AGCT 6 cut(s) 11, 149, 184, 493, 687, 1006
Alw21I GWGCWC 2 cut(s) 151, 332
Alw26I GTCTC 3 cut(s) 88, 293, 651
AlwI GGATC 1 cut(s) 893
AlwNI CAGNNNCTG 1 cut(s) 308
Ama87I CYCGRG 1 cut(s) 388
AoxI GGCC 6 cut(s) 48, 230, 267, 358, 420, 559
ApaI GGGCCC 1 cut(s) 234
ApeKI GCWGC 4 cut(s) 208, 211, 292, 425
ApoI RAATTY 1 cut(s) 1038
ArsI GACNNNNNNTTYG 2 cut(s) 650, 682
Asp700I GAANNNNTTC 2 cut(s) 1165, 1197
AspS9I GGNCC 6 cut(s) 48, 230, 231, 267, 559, 1068
AsuHPI GGTGA 3 cut(s) 410, 479, 1100
AsuII TTCGAA 1 cut(s) 1159
AsuNHI GCTAGC 1 cut(s) 514
AvaI CYCGRG 1 cut(s) 388
AvaII GGWCC 1 cut(s) 1068
AxyI CCTNAGG 1 cut(s) 756
BaeGI GKGCMC 3 cut(s) 234, 409, 451
BanI GGYRCC 2 cut(s) 446, 805
BanII GRGCYC 2 cut(s) 151, 234
BbrPI CACGTG 1 cut(s) 529
Bbv12I GWGCWC 2 cut(s) 151, 332
BbvI GCAGC 4 cut(s) 195, 198, 304, 437
BccI CCATC 5 cut(s) 95, 314, 573, 1021, 1101
BceAI ACGGC 5 cut(s) 35, 41, 345, 503, 955
BciT130I CCWGG 3 cut(s) 452, 604, 1086
BcoDI GTCTC 3 cut(s) 88, 293, 651
BcuI ACTAGT 2 cut(s) 332, 976
BfaI CTAG 3 cut(s) 333, 515, 977
BfmI CTRYAG 2 cut(s) 309, 1007
BisI GCNGC 6 cut(s) 209, 212, 293, 358, 423, 426
BlsI GCNGC 6 cut(s) 210, 213, 294, 359, 424, 427
Bme1390I CCNGG 3 cut(s) 452, 604, 1086
Bme18I GGWCC 1 cut(s) 1068
BmeRI GACNNNNNGTC 1 cut(s) 740
BmeT110I CYCGRG 1 cut(s) 388
BmgBI CACGTC 3 cut(s) 45, 413, 1118
BmgT120I GGNCC 6 cut(s) 48, 230, 231, 267, 559, 1068
BmiI GGNNCC 9 cut(s) 200, 232, 268, 448, 695, 807, 1069, 1070, 1083
BmrFI CCNGG 3 cut(s) 452, 604, 1086
BmsI GCATC 1 cut(s) 286
BmtI GCTAGC 1 cut(s) 518
Bpu14I TTCGAA 1 cut(s) 1159
BpuEI CTTGAG 1 cut(s) 703
BsaAI YACGTR 1 cut(s) 529
BsaI GGTCTC 1 cut(s) 88
BsaJI CCNNGG 3 cut(s) 450, 451, 937
BsaWI WCCGGW 2 cut(s) 395, 1208
BsaXI ACNNNNNCTCC 2 cut(s) 1044, 1074
Bsc4I CCNNNNNNNGG 1 cut(s) 1078
Bse1I ACTGG 5 cut(s) 168, 555, 619, 697, 1194
Bse21I CCTNAGG 1 cut(s) 756
BseBI CCWGG 3 cut(s) 452, 604, 1086
BseDI CCNNGG 3 cut(s) 450, 451, 937
BseGI GGATG 4 cut(s) 277, 325, 1134, 1169
BseLI CCNNNNNNNGG 1 cut(s) 1078
BseMII CTCAG 1 cut(s) 824
BseNI ACTGG 5 cut(s) 168, 555, 619, 697, 1194
BseRI GAGGAG 2 cut(s) 329, 589
BseSI GKGCMC 3 cut(s) 234, 409, 451
BseX3I CGGCCG 1 cut(s) 358
BseXI GCAGC 4 cut(s) 195, 198, 304, 437
Bsh1285I CGRYCG 1 cut(s) 361
BshFI GGCC 6 cut(s) 50, 232, 269, 360, 422, 561
BshNI GGYRCC 2 cut(s) 446, 805
BsiEI CGRYCG 1 cut(s) 361
BsiHKAI GWGCWC 2 cut(s) 151, 332
BsiHKCI CYCGRG 1 cut(s) 388
BsiSI CCGG 3 cut(s) 396, 429, 1209
BslFI GGGAC 3 cut(s) 1012, 1044, 1081
BslI CCNNNNNNNGG 1 cut(s) 1078
BsmAI GTCTC 3 cut(s) 88, 293, 651
BsmFI GGGAC 3 cut(s) 1012, 1044, 1081
BsnI GGCC 6 cut(s) 50, 232, 269, 360, 422, 561
Bso31I GGTCTC 1 cut(s) 88
BsoBI CYCGRG 1 cut(s) 388
Bsp119I TTCGAA 1 cut(s) 1159
Bsp120I GGGCCC 1 cut(s) 230
Bsp1286I GDGCHC 5 cut(s) 151, 234, 332, 409, 451
Bsp143I GATC 7 cut(s) 23, 114, 746, 851, 885, 897, 984
BspACI CCGC 5 cut(s) 357, 380, 423, 444, 1073
BspANI GGCC 6 cut(s) 50, 232, 269, 360, 422, 561
BspCNI CTCAG 1 cut(s) 823
BspHI TCATGA 1 cut(s) 981
BspLI GGNNCC 9 cut(s) 200, 232, 268, 448, 695, 807, 1069, 1070, 1083
BspMAI CTGCAG 1 cut(s) 313
BspOI GCTAGC 1 cut(s) 518
BspPI GGATC 1 cut(s) 893
BspT104I TTCGAA 1 cut(s) 1159
BspT107I GGYRCC 2 cut(s) 446, 805
BspTNI GGTCTC 1 cut(s) 88
BsrI ACTGG 5 cut(s) 168, 555, 619, 697, 1194
BssECI CCNNGG 3 cut(s) 450, 451, 937
BssMI GATC 7 cut(s) 23, 114, 746, 851, 885, 897, 984
Bst2UI CCWGG 3 cut(s) 452, 604, 1086
Bst4CI ACNGT 5 cut(s) 220, 304, 610, 738, 1184
Bst6I CTCTTC 2 cut(s) 855, 1103
BstBAI YACGTR 1 cut(s) 529
BstBI TTCGAA 1 cut(s) 1159
BstC8I GCNNGC 1 cut(s) 516
BstDEI CTNAG 2 cut(s) 756, 810
BstDSI CCRYGG 1 cut(s) 937
BstEII GGTNACC 1 cut(s) 1088
BstF5I GGATG 4 cut(s) 277, 325, 1134, 1169
BstKTI GATC 7 cut(s) 26, 117, 749, 854, 888, 900, 987
BstMAI GTCTC 3 cut(s) 88, 293, 651
BstMBI GATC 7 cut(s) 23, 114, 746, 851, 885, 897, 984
BstMCI CGRYCG 1 cut(s) 361
BstMWI GCNNNNNNNGC 3 cut(s) 693, 803, 1081
BstNI CCWGG 3 cut(s) 452, 604, 1086
BstPI GGTNACC 1 cut(s) 1088
BstSCI CCNGG 3 cut(s) 450, 602, 1084
BstSFI CTRYAG 2 cut(s) 309, 1007
BstSLI GKGCMC 3 cut(s) 234, 409, 451
BstV1I GCAGC 4 cut(s) 195, 198, 304, 437
BstZI CGGCCG 1 cut(s) 358
Bsu36I CCTNAGG 1 cut(s) 756
BsuRI GGCC 6 cut(s) 50, 232, 269, 360, 422, 561
BtgI CCRYGG 1 cut(s) 937
BtrI CACGTC 3 cut(s) 45, 413, 1118
BtsCI GGATG 4 cut(s) 277, 325, 1134, 1169
BtsI GCAGTG 1 cut(s) 306
BtsIMutI CAGTG 5 cut(s) 161, 306, 548, 756, 1187
Cac8I GCNNGC 1 cut(s) 516
CaiI CAGNNNCTG 1 cut(s) 308
CciI TCATGA 1 cut(s) 981
Cfr13I GGNCC 6 cut(s) 48, 230, 231, 267, 559, 1068
CseI GACGC 1 cut(s) 781
Csp6I GTAC 2 cut(s) 841, 1185
CviAII CATG 2 cut(s) 282, 982
CviQI GTAC 2 cut(s) 841, 1185
DdeI CTNAG 2 cut(s) 756, 810
DpnI GATC 7 cut(s) 25, 116, 748, 853, 887, 899, 986
DpnII GATC 7 cut(s) 23, 114, 746, 851, 885, 897, 984
DriI GACNNNNNGTC 1 cut(s) 740
EaeI YGGCCR 2 cut(s) 358, 420
EagI CGGCCG 1 cut(s) 358
Eam1104I CTCTTC 2 cut(s) 855, 1103
Eam1105I GACNNNNNGTC 1 cut(s) 740
EarI CTCTTC 2 cut(s) 855, 1103
Ecl136II GAGCTC 1 cut(s) 149
EclXI CGGCCG 1 cut(s) 358
Eco24I GRGCYC 2 cut(s) 151, 234
Eco31I GGTCTC 1 cut(s) 88
Eco47I GGWCC 1 cut(s) 1068
Eco52I CGGCCG 1 cut(s) 358
Eco53kI GAGCTC 1 cut(s) 149
Eco57I CTGAAG 1 cut(s) 715
Eco72I CACGTG 1 cut(s) 529
Eco81I CCTNAGG 1 cut(s) 756
Eco88I CYCGRG 1 cut(s) 388
Eco91I GGTNACC 1 cut(s) 1088
EcoICRI GAGCTC 1 cut(s) 149
EcoO109I RGGNCCY 2 cut(s) 230, 1068
EcoO65I GGTNACC 1 cut(s) 1088
EcoRII CCWGG 3 cut(s) 450, 602, 1084
EcoT38I GRGCYC 2 cut(s) 151, 234
FaeI CATG 2 cut(s) 285, 985
FalI AAGNNNNNCTT 2 cut(s) 168, 200
FaqI GGGAC 3 cut(s) 1012, 1044, 1081
FatI CATG 2 cut(s) 281, 981
FauI CCCGC 1 cut(s) 1080
FblI GTMKAC 1 cut(s) 612
Fnu4HI GCNGC 6 cut(s) 209, 212, 293, 358, 423, 426
FokI GGATG 4 cut(s) 264, 332, 1121, 1156
FriOI GRGCYC 2 cut(s) 151, 234
Fsp4HI GCNGC 6 cut(s) 209, 212, 293, 358, 423, 426
FspBI CTAG 3 cut(s) 333, 515, 977
GluI GCNGC 6 cut(s) 209, 212, 293, 358, 423, 426
HaeIII GGCC 6 cut(s) 50, 232, 269, 360, 422, 561
HapII CCGG 3 cut(s) 396, 429, 1209
HgaI GACGC 1 cut(s) 781
Hin1II CATG 2 cut(s) 285, 985
HincII GTYRAC 1 cut(s) 702
HindII GTYRAC 1 cut(s) 702
HinfI GANTC 4 cut(s) 341, 484, 642, 814
HpaII CCGG 3 cut(s) 396, 429, 1209
HphI GGTGA 3 cut(s) 410, 479, 1100
Hpy166II GTNNAC 3 cut(s) 613, 702, 1187
Hpy188I TCNGA 8 cut(s) 119, 241, 346, 647, 734, 746, 813, 897
Hpy188III TCNNGA 5 cut(s) 338, 388, 835, 982, 1043
Hpy8I GTNNAC 3 cut(s) 613, 702, 1187
Hpy99I CGWCG 2 cut(s) 46, 414
HpyAV CCTTC 3 cut(s) 116, 360, 669
HpyCH4III ACNGT 5 cut(s) 220, 304, 610, 738, 1184
HpyCH4IV ACGT 5 cut(s) 44, 412, 528, 946, 1117
HpyCH4V TGCA 4 cut(s) 249, 311, 540, 797
HpyF10VI GCNNNNNNNGC 3 cut(s) 693, 803, 1081
HpyF3I CTNAG 2 cut(s) 756, 810
HpySE526I ACGT 5 cut(s) 44, 412, 528, 946, 1117
Hsp92II CATG 2 cut(s) 285, 985
KflI GGGWCCC 1 cut(s) 1068
Kzo9I GATC 7 cut(s) 23, 114, 746, 851, 885, 897, 984
LmnI GCTCC 3 cut(s) 31, 693, 1081
Lsp1109I GCAGC 4 cut(s) 195, 198, 304, 437
LweI GCATC 1 cut(s) 286
MaeI CTAG 3 cut(s) 333, 515, 977
MaeII ACGT 5 cut(s) 44, 412, 528, 946, 1117
MaeIII GTNAC 2 cut(s) 304, 1088
MalI GATC 7 cut(s) 25, 116, 748, 853, 887, 899, 986
MboI GATC 7 cut(s) 23, 114, 746, 851, 885, 897, 984
MboII GAAGA 6 cut(s) 148, 499, 721, 872, 1090, 1118
MhlI GDGCHC 5 cut(s) 151, 234, 332, 409, 451
MluCI AATT 3 cut(s) 593, 1038, 1194
MlyI GAGTC 1 cut(s) 823
MmeI TCCRAC 1 cut(s) 108
MnlI CCTC 8 cut(s) 212, 307, 310, 567, 819, 856, 903, 1041
MroXI GAANNNNTTC 2 cut(s) 1165, 1197
MseI TTAA 3 cut(s) 846, 1035, 1062
MslI CAYNNNNRTG 1 cut(s) 509
MspA1I CMGCKG 1 cut(s) 357
MspI CCGG 3 cut(s) 396, 429, 1209
MspR9I CCNGG 3 cut(s) 452, 604, 1086
MvaI CCWGG 3 cut(s) 452, 604, 1086
MwoI GCNNNNNNNGC 3 cut(s) 693, 803, 1081
NdeII GATC 7 cut(s) 23, 114, 746, 851, 885, 897, 984
NheI GCTAGC 1 cut(s) 514
NlaIII CATG 2 cut(s) 285, 985
NlaIV GGNNCC 9 cut(s) 200, 232, 268, 448, 695, 807, 1069, 1070, 1083
NmeAIII GCCGAG 1 cut(s) 587
NmuCI GTSAC 2 cut(s) 304, 1088
NspV TTCGAA 1 cut(s) 1159
PaeR7I CTCGAG 1 cut(s) 388
PagI TCATGA 1 cut(s) 981
PasI CCCWGGG 1 cut(s) 451
PcsI WCGNNNNNNNCGW 1 cut(s) 516
PdmI GAANNNNTTC 2 cut(s) 1165, 1197
PfeI GAWTC 3 cut(s) 341, 484, 642
PkrI GCNGC 6 cut(s) 210, 213, 294, 359, 424, 427
PleI GAGTC 1 cut(s) 822
PmaCI CACGTG 1 cut(s) 529
PmlI CACGTG 1 cut(s) 529
PpsI GAGTC 1 cut(s) 822
Ppu21I YACGTR 1 cut(s) 529
PpuMI RGGWCCY 1 cut(s) 1068
PsiI TTATAA 1 cut(s) 1016
Psp124BI GAGCTC 1 cut(s) 151
Psp5II RGGWCCY 1 cut(s) 1068
Psp6I CCWGG 3 cut(s) 450, 602, 1084
PspCI CACGTG 1 cut(s) 529
PspEI GGTNACC 1 cut(s) 1088
PspGI CCWGG 3 cut(s) 450, 602, 1084
PspN4I GGNNCC 9 cut(s) 200, 232, 268, 448, 695, 807, 1069, 1070, 1083
PspOMI GGGCCC 1 cut(s) 230
PspPI GGNCC 6 cut(s) 48, 230, 231, 267, 559, 1068
PspPPI RGGWCCY 1 cut(s) 1068
PstI CTGCAG 1 cut(s) 313
PstNI CAGNNNCTG 1 cut(s) 308
RsaI GTAC 2 cut(s) 842, 1186
RsaNI GTAC 2 cut(s) 841, 1185
RseI CAYNNNNRTG 1 cut(s) 509
SacI GAGCTC 1 cut(s) 151
SaqAI TTAA 3 cut(s) 846, 1035, 1062
SatI GCNGC 6 cut(s) 209, 212, 293, 358, 423, 426
Sau3AI GATC 7 cut(s) 23, 114, 746, 851, 885, 897, 984
Sau96I GGNCC 6 cut(s) 48, 230, 231, 267, 559, 1068
SchI GAGTC 1 cut(s) 823
ScrFI CCNGG 3 cut(s) 452, 604, 1086
SduI GDGCHC 5 cut(s) 151, 234, 332, 409, 451
SfaNI GCATC 1 cut(s) 286
SfcI CTRYAG 2 cut(s) 309, 1007
Sfr274I CTCGAG 1 cut(s) 388
SfuI TTCGAA 1 cut(s) 1159
SinI GGWCC 1 cut(s) 1068
SlaI CTCGAG 1 cut(s) 388
SmiMI CAYNNNNRTG 1 cut(s) 509
SmlI CTYRAG 2 cut(s) 388, 682
SmoI CTYRAG 2 cut(s) 388, 682
SpeI ACTAGT 2 cut(s) 332, 976
Sse9I AATT 3 cut(s) 593, 1038, 1194
SsiI CCGC 5 cut(s) 357, 380, 423, 444, 1073
SspMI CTAG 3 cut(s) 333, 515, 977
SstI GAGCTC 1 cut(s) 151
StyD4I CCNGG 3 cut(s) 450, 602, 1084
TaaI ACNGT 5 cut(s) 220, 304, 610, 738, 1184
TaiI ACGT 5 cut(s) 47, 415, 531, 949, 1120
TaqI TCGA 4 cut(s) 96, 389, 762, 1159
TasI AATT 3 cut(s) 593, 1038, 1194
TatI WGTACW 2 cut(s) 840, 1184
TauI GCSGC 2 cut(s) 360, 425
TfiI GAWTC 3 cut(s) 341, 484, 642
Tru1I TTAA 3 cut(s) 846, 1035, 1062
Tru9I TTAA 3 cut(s) 846, 1035, 1062
TscAI CASTG 5 cut(s) 168, 313, 555, 756, 1194
TseFI GTSAC 2 cut(s) 304, 1088
TseI GCWGC 4 cut(s) 208, 211, 292, 425
Tsp45I GTSAC 2 cut(s) 304, 1088
TspDTI ATGAA 4 cut(s) 98, 485, 939, 1158
TspGWI ACGGA 1 cut(s) 453
TspRI CASTG 5 cut(s) 168, 313, 555, 756, 1194
VpaK11BI GGWCC 1 cut(s) 1068
XapI RAATTY 1 cut(s) 1038
XhoI CTCGAG 1 cut(s) 388
XmiI GTMKAC 1 cut(s) 612
XmnI GAANNNNTTC 2 cut(s) 1165, 1197
XspI CTAG 3 cut(s) 333, 515, 977
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.