RchiOBHm_Chr5g0059871

May be involved in modulation of pathogen defense and leaf cell death

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000744
Physical Location & Seq
Reverse (-)
64937188 .. 64943805
6618 bp
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UTR
Exon/CDS
Intron
PRQ33645

Sequence Viewer

Length: 1776 bp
ATGGCGGCATCAACCTCCGGAAGATCGTTGGAGCAAACGCCGACATGGGCCGTCGCCGTTGTCTGTTTTGTTTTGGTTCTAGTCTCAATCATCATCGAATACATAATCGAACTTATAGCAAAGTGGTTGAAGAGGAAACACAAACGAGCTCTTTTCGAAGCACTAGAAAAGATCAAATCAGAGCTTATGCTATTGGGGTTTATATCGCTGCTGCTGACGGTGGGGCAAGGTCCGATATCGAACATATGCATATCGAAGAAAGTGGGAAACACGTGGCATCCGTGTAGCAAGAAGCAGGAGGAAAAGATCGTGGAAGAAGAAGAGGTTGAAAGCCCAAGTAGGAAACTGCTGGCAGCGTTTGAATCTGGTTATGGAGGTGGAAGCGCACGCCGCGTTTTGGCGGCTGCGGGCACCGACAAATGTGCAGCCAAGGGAAAAGTTCCATTCATATCTGCGGATGGTATTCATCAACTGCATATATTTATCTTCGTCTTAGCTGTTTTCCACGTACTCTATTGCATTCTCACTATGGCTTTAGGAAGAGCCAAGATGAGAAGTTGGAAGAGGTGGGAAAAAGAAACAAGAACGGCTGAATATCAATTTTCGCACGATCCAGAGCGGTTCAGATTTGCAAGGGACACATCTTTTGGGAGAAGACATTTAAGTTACTGGACCAAAACACCTTTTCTCATGTGGATTGTTTGTTTTTTCAGACAATTTGTTAGATCAGTTCCCAAAGTTGATTACTTGACCTTGCGCCACGGGTTTATCATGGCACATTTGGCACCTCAAAGCCACCAAAAATTCAATTTCCAAAAGTACATAAACAGATCACTCGAAGAGGATTTCAAGGTGGTAGTGGGAATCAGCCCTCCAATTTGGGGTTTTGCCGTGGTCTTTCTACTCTTTAACACTCATGGCTGGTATTCTTATCTTTGGCTACCCTTTATCCCACTAATTATCATTCTATTGGTGGGAACCAAACTACAAGTTATCATAACCAAAATGGGTCTTAGAATCCAAGAAAGAGGAGAAGTTGTGAAGGGTACACCAGTTGTTCAACCTGGTGATGACCTCTTCTGGTTTAACCGCCCTCGCCTCATTCTCTACCTCATCAACTTCGTTCTATTTCAGAATGCCTTCCAACTTGCGTTCTTTGCATGGAGTTGGTATGAATTTTCTTTGAAATCTTGCTTTCACGAGCATACTGAGGATATCATCATCAGAGTTTCAATGGGGGTCCTCATACAAATTCTCTGCAGTTATGTCACTCTCCCACTTTATGCCCTTGTCACACAGATGGGCTCTTCTATGAAACCAACAATATTCAACGAGAGGGTAGCCACAGCTCTACGCAACTGGCATCACACGGCTAAGAAGCACATCAAACAGAACAAAGGCTCCGTAACTCCAATGTCTAGCCGACCAGGCACTCCATCCCACCATGCTTCCCCCATCCACCTCCTCCGGCATTACCGCAGCGAGGTGGACAGCTTCCACACATCACCGAGAAGATCAAACTTTGAAGTTGAACAACGTTATGAAACCGACTCACCCTCCCCCTCGCACCACCTCCACATAGATGGTTCGTTCTCTCACCAACACCAAGTCGAAGTAGGGAACATGGAACATGATCGGGTGGATGCCAACGAACCCAACTCGGTCCATGCGGCTCCTGCTAGCGTTAGAGGTTCGCAATCGACTCCCGCCCAACACGAAATTAATATTGGACCGCCCAAGGACTTTTCATTTGATAAGAGACAAGGTGCTGTATAA

Protein Analysis

591

Amino Acids

67.65

Weight (kDa)

9.5

Isoelectric Point (pI)

48.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mlo PF03094 7 - 492 1.5e-250 Mlo family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 410, 784
AccBSI CCGCTC 1 cut(s) 619
AccII CGCG 1 cut(s) 393
AccIII TCCGGA 1 cut(s) 17
AclI AACGTT 1 cut(s) 1537
AclWI GGATC 1 cut(s) 605
AcsI RAATTY 3 cut(s) 803, 1175, 1251
AcvI CACGTG 1 cut(s) 273
AfaI GTAC 3 cut(s) 510, 821, 1048
AfiI CCNNNNNNNGG 3 cut(s) 397, 881, 1483
AflIII ACRYGT 1 cut(s) 270
AjnI CCWGG 2 cut(s) 1063, 1426
AjuI GAANNNNNNNTTGG 2 cut(s) 1710, 1742
AloI GAACNNNNNNTCC 2 cut(s) 1385, 1417
AluBI AGCT 5 cut(s) 149, 184, 497, 1349, 1494
AluI AGCT 5 cut(s) 149, 184, 497, 1349, 1494
Alw21I GWGCWC 1 cut(s) 151
Alw26I GTCTC 2 cut(s) 88, 1753
AlwI GGATC 1 cut(s) 605
Aor13HI TCCGGA 1 cut(s) 17
AoxI GGCC 1 cut(s) 48
ApeKI GCWGC 6 cut(s) 208, 211, 353, 404, 425, 1479
ApoI RAATTY 3 cut(s) 803, 1175, 1251
ArsI GACNNNNNNTTYG 4 cut(s) 629, 661, 1733, 1765
AseI ATTAAT 1 cut(s) 1722
Asp700I GAANNNNTTC 1 cut(s) 1139
AspLEI GCGC 2 cut(s) 386, 759
AspS9I GGNCC 6 cut(s) 48, 230, 672, 1240, 1663, 1730
AsuHPI GGTGA 4 cut(s) 1079, 1497, 1545, 1589
AsuII TTCGAA 1 cut(s) 156
AsuNHI GCTAGC 1 cut(s) 1679
AvaII GGWCC 5 cut(s) 230, 672, 1240, 1663, 1730
BaeGI GKGCMC 1 cut(s) 413
BaeI ACNNNNGTAYC 2 cut(s) 1038, 1071
BanI GGYRCC 2 cut(s) 410, 784
BanII GRGCYC 2 cut(s) 151, 1307
BauI CACGAG 1 cut(s) 1199
BbrPI CACGTG 1 cut(s) 273
BbsI GAAGAC 1 cut(s) 661
Bbv12I GWGCWC 1 cut(s) 151
BbvI GCAGC 6 cut(s) 195, 198, 365, 391, 437, 1491
BccI CCATC 5 cut(s) 452, 1294, 1444, 1463, 1577
BceAI ACGGC 5 cut(s) 35, 41, 603, 875, 1386
BcgI CGANNNNNNTGC 2 cut(s) 404, 438
BciT130I CCWGG 2 cut(s) 1065, 1428
BcoDI GTCTC 2 cut(s) 88, 1753
BfaI CTAG 4 cut(s) 80, 164, 1419, 1680
BfmI CTRYAG 1 cut(s) 1258
BglI GCCNNNNNGGC 1 cut(s) 1428
Bme1390I CCNGG 2 cut(s) 1065, 1428
Bme18I GGWCC 5 cut(s) 230, 672, 1240, 1663, 1730
BmgT120I GGNCC 6 cut(s) 48, 230, 672, 1240, 1663, 1730
BmiI GGNNCC 6 cut(s) 412, 786, 979, 1241, 1402, 1674
BmrFI CCNGG 2 cut(s) 1065, 1428
BmsI GCATC 4 cut(s) 17, 286, 1372, 1633
BmtI GCTAGC 1 cut(s) 1683
BpiI GAAGAC 1 cut(s) 661
Bpu14I TTCGAA 1 cut(s) 156
BsaAI YACGTR 2 cut(s) 273, 508
BsaJI CCNNGG 4 cut(s) 429, 760, 891, 1737
BsaWI WCCGGW 1 cut(s) 17
BsaXI ACNNNNNCTCC 6 cut(s) 1023, 1053, 1385, 1415, 1541, 1571
Bsc4I CCNNNNNNNGG 3 cut(s) 397, 881, 1483
Bse1I ACTGG 3 cut(s) 674, 1052, 1364
BseAI TCCGGA 1 cut(s) 17
BseBI CCWGG 2 cut(s) 1065, 1428
BseDI CCNNGG 4 cut(s) 429, 760, 891, 1737
BseGI GGATG 5 cut(s) 277, 463, 1436, 1455, 1648
BseLI CCNNNNNNNGG 3 cut(s) 397, 881, 1483
BseMII CTCAG 1 cut(s) 1200
BseNI ACTGG 3 cut(s) 674, 1052, 1364
BseRI GAGGAG 2 cut(s) 1044, 1454
BseSI GKGCMC 1 cut(s) 413
BseXI GCAGC 6 cut(s) 195, 198, 365, 391, 437, 1491
BsgI GTGCAG 1 cut(s) 444
Bsh1236I CGCG 1 cut(s) 393
BshFI GGCC 1 cut(s) 50
BshNI GGYRCC 2 cut(s) 410, 784
BsiHKAI GWGCWC 1 cut(s) 151
BsiSI CCGG 2 cut(s) 18, 1468
BslFI GGGAC 1 cut(s) 650
BslI CCNNNNNNNGG 3 cut(s) 397, 881, 1483
BsmAI GTCTC 2 cut(s) 88, 1753
BsmFI GGGAC 1 cut(s) 650
BsmI GAATGC 2 cut(s) 519, 1141
BsnI GGCC 1 cut(s) 50
Bsp119I TTCGAA 1 cut(s) 156
Bsp1286I GDGCHC 3 cut(s) 151, 413, 1307
Bsp13I TCCGGA 1 cut(s) 17
Bsp143I GATC 8 cut(s) 23, 171, 306, 610, 725, 830, 1514, 1633
BspANI GGCC 1 cut(s) 50
BspCNI CTCAG 1 cut(s) 1201
BspEI TCCGGA 1 cut(s) 17
BspFNI CGCG 1 cut(s) 393
BspLI GGNNCC 6 cut(s) 412, 786, 979, 1241, 1402, 1674
BspMAI CTGCAG 1 cut(s) 1262
BspOI GCTAGC 1 cut(s) 1683
BspPI GGATC 1 cut(s) 605
BspQI GCTCTTC 2 cut(s) 535, 1312
BspT104I TTCGAA 1 cut(s) 156
BspT107I GGYRCC 2 cut(s) 410, 784
BsrBI CCGCTC 1 cut(s) 619
BsrI ACTGG 3 cut(s) 674, 1052, 1364
BssECI CCNNGG 4 cut(s) 429, 760, 891, 1737
BssMI GATC 8 cut(s) 23, 171, 306, 610, 725, 830, 1514, 1633
BssSI CACGAG 1 cut(s) 1199
BssT1I CCWWGG 2 cut(s) 429, 1737
Bst2BI CACGAG 1 cut(s) 1199
Bst2UI CCWGG 2 cut(s) 1065, 1428
Bst4CI ACNGT 1 cut(s) 220
Bst6I CTCTTC 7 cut(s) 125, 315, 535, 557, 834, 1082, 1312
BstBAI YACGTR 2 cut(s) 273, 508
BstBI TTCGAA 1 cut(s) 156
BstC8I GCNNGC 4 cut(s) 351, 388, 409, 1681
BstDEI CTNAG 4 cut(s) 493, 1013, 1209, 1374
BstDSI CCRYGG 2 cut(s) 760, 891
BstF5I GGATG 5 cut(s) 277, 463, 1436, 1455, 1648
BstFNI CGCG 1 cut(s) 393
BstHHI GCGC 2 cut(s) 386, 759
BstKTI GATC 8 cut(s) 26, 174, 309, 613, 728, 833, 1517, 1636
BstMAI GTCTC 2 cut(s) 88, 1753
BstMBI GATC 8 cut(s) 23, 171, 306, 610, 725, 830, 1514, 1633
BstMWI GCNNNNNNNGC 5 cut(s) 390, 782, 1157, 1428, 1676
BstNI CCWGG 2 cut(s) 1065, 1428
BstSCI CCNGG 2 cut(s) 1063, 1426
BstSFI CTRYAG 1 cut(s) 1258
BstSLI GKGCMC 1 cut(s) 413
BstUI CGCG 1 cut(s) 393
BstV1I GCAGC 6 cut(s) 195, 198, 365, 391, 437, 1491
BstV2I GAAGAC 1 cut(s) 661
BstXI CCANNNNNNTGG 1 cut(s) 1583
BsuRI GGCC 1 cut(s) 50
BtgI CCRYGG 2 cut(s) 760, 891
BtsCI GGATG 5 cut(s) 277, 463, 1436, 1455, 1648
Cac8I GCNNGC 4 cut(s) 351, 388, 409, 1681
CfoI GCGC 2 cut(s) 386, 759
Cfr13I GGNCC 6 cut(s) 48, 230, 672, 1240, 1663, 1730
CsiI ACCWGGT 1 cut(s) 1063
Csp6I GTAC 3 cut(s) 509, 820, 1047
CviAII CATG 9 cut(s) 45, 691, 772, 917, 1161, 1445, 1624, 1631, 1667
CviQI GTAC 3 cut(s) 509, 820, 1047
DdeI CTNAG 4 cut(s) 493, 1013, 1209, 1374
DpnI GATC 8 cut(s) 25, 173, 308, 612, 727, 832, 1516, 1635
DpnII GATC 8 cut(s) 23, 171, 306, 610, 725, 830, 1514, 1633
Eam1104I CTCTTC 7 cut(s) 125, 315, 535, 557, 834, 1082, 1312
EarI CTCTTC 7 cut(s) 125, 315, 535, 557, 834, 1082, 1312
Ecl136II GAGCTC 1 cut(s) 149
Eco130I CCWWGG 2 cut(s) 429, 1737
Eco24I GRGCYC 2 cut(s) 151, 1307
Eco32I GATATC 2 cut(s) 237, 1216
Eco47I GGWCC 5 cut(s) 230, 672, 1240, 1663, 1730
Eco53kI GAGCTC 1 cut(s) 149
Eco72I CACGTG 1 cut(s) 273
EcoICRI GAGCTC 1 cut(s) 149
EcoO109I RGGNCCY 1 cut(s) 1240
EcoRII CCWGG 2 cut(s) 1063, 1426
EcoRV GATATC 2 cut(s) 237, 1216
EcoT14I CCWWGG 2 cut(s) 429, 1737
EcoT22I ATGCAT 1 cut(s) 251
EcoT38I GRGCYC 2 cut(s) 151, 1307
ErhI CCWWGG 2 cut(s) 429, 1737
FaeI CATG 9 cut(s) 48, 694, 775, 920, 1164, 1448, 1627, 1634, 1670
FaqI GGGAC 1 cut(s) 650
FatI CATG 9 cut(s) 44, 690, 771, 916, 1160, 1444, 1623, 1630, 1666
FauI CCCGC 2 cut(s) 400, 1714
FauNDI CATATG 1 cut(s) 245
FokI GGATG 5 cut(s) 264, 470, 1423, 1442, 1655
FriOI GRGCYC 2 cut(s) 151, 1307
FspBI CTAG 4 cut(s) 80, 164, 1419, 1680
GlaI GCGC 2 cut(s) 385, 758
HaeIII GGCC 1 cut(s) 50
HapII CCGG 2 cut(s) 18, 1468
HhaI GCGC 2 cut(s) 386, 759
Hin1II CATG 9 cut(s) 48, 694, 775, 920, 1164, 1448, 1627, 1634, 1670
Hin6I GCGC 2 cut(s) 384, 757
HinP1I GCGC 2 cut(s) 384, 757
HinfI GANTC 5 cut(s) 362, 864, 1017, 1550, 1702
HpaII CCGG 2 cut(s) 18, 1468
HphI GGTGA 4 cut(s) 1079, 1497, 1545, 1589
Hpy166II GTNNAC 2 cut(s) 1049, 1489
Hpy188I TCNGA 6 cut(s) 181, 234, 626, 713, 1134, 1226
Hpy188III TCNNGA 3 cut(s) 18, 614, 1199
Hpy8I GTNNAC 2 cut(s) 1049, 1489
Hpy99I CGWCG 1 cut(s) 56
HpyAV CCTTC 2 cut(s) 1036, 1150
HpyCH4III ACNGT 1 cut(s) 220
HpyCH4IV ACGT 3 cut(s) 272, 507, 1537
HpyCH4V TGCA 7 cut(s) 249, 425, 475, 519, 632, 1160, 1260
HpyF10VI GCNNNNNNNGC 5 cut(s) 390, 782, 1157, 1428, 1676
HpyF3I CTNAG 4 cut(s) 493, 1013, 1209, 1374
HpySE526I ACGT 3 cut(s) 272, 507, 1537
Hsp92II CATG 9 cut(s) 48, 694, 775, 920, 1164, 1448, 1627, 1634, 1670
HspAI GCGC 2 cut(s) 384, 757
Kpn2I TCCGGA 1 cut(s) 17
Kzo9I GATC 8 cut(s) 23, 171, 306, 610, 725, 830, 1514, 1633
LguI GCTCTTC 2 cut(s) 535, 1312
LmnI GCTCC 3 cut(s) 31, 1406, 1678
Lsp1109I GCAGC 6 cut(s) 195, 198, 365, 391, 437, 1491
LweI GCATC 4 cut(s) 17, 286, 1372, 1633
MabI ACCWGGT 1 cut(s) 1063
MaeI CTAG 4 cut(s) 80, 164, 1419, 1680
MaeII ACGT 3 cut(s) 272, 507, 1537
MaeIII GTNAC 4 cut(s) 665, 1267, 1291, 1405
MalI GATC 8 cut(s) 25, 173, 308, 612, 727, 832, 1516, 1635
MbiI CCGCTC 1 cut(s) 619
MboI GATC 8 cut(s) 23, 171, 306, 610, 725, 830, 1514, 1633
MhlI GDGCHC 3 cut(s) 151, 413, 1307
MluCI AATT 9 cut(s) 599, 716, 803, 808, 876, 957, 1175, 1251, 1719
MlyI GAGTC 2 cut(s) 1544, 1696
MmeI TCCRAC 3 cut(s) 9, 539, 1168
Mph1103I ATGCAT 1 cut(s) 251
MroI TCCGGA 1 cut(s) 17
MroXI GAANNNNTTC 1 cut(s) 1139
MseI TTAA 4 cut(s) 662, 909, 1086, 1722
MslI CAYNNNNRTG 2 cut(s) 1298, 1581
MspI CCGG 2 cut(s) 18, 1468
MspR9I CCNGG 2 cut(s) 1065, 1428
Mva1269I GAATGC 2 cut(s) 519, 1141
MvaI CCWGG 2 cut(s) 1065, 1428
MvnI CGCG 1 cut(s) 393
MwoI GCNNNNNNNGC 5 cut(s) 390, 782, 1157, 1428, 1676
NdeI CATATG 1 cut(s) 245
NdeII GATC 8 cut(s) 23, 171, 306, 610, 725, 830, 1514, 1633
NheI GCTAGC 1 cut(s) 1679
NlaIII CATG 9 cut(s) 48, 694, 775, 920, 1164, 1448, 1627, 1634, 1670
NlaIV GGNNCC 6 cut(s) 412, 786, 979, 1241, 1402, 1674
NmuCI GTSAC 2 cut(s) 1267, 1291
NsiI ATGCAT 1 cut(s) 251
NspV TTCGAA 1 cut(s) 156
PciSI GCTCTTC 2 cut(s) 535, 1312
PcsI WCGNNNNNNNCGW 1 cut(s) 278
PctI GAATGC 2 cut(s) 519, 1141
PdmI GAANNNNTTC 1 cut(s) 1139
PfeI GAWTC 3 cut(s) 362, 864, 1017
PleI GAGTC 2 cut(s) 1544, 1696
PmaCI CACGTG 1 cut(s) 273
PmlI CACGTG 1 cut(s) 273
PpsI GAGTC 2 cut(s) 1544, 1696
Ppu21I YACGTR 2 cut(s) 273, 508
PpuMI RGGWCCY 1 cut(s) 1240
PshBI ATTAAT 1 cut(s) 1722
Psp124BI GAGCTC 1 cut(s) 151
Psp1406I AACGTT 1 cut(s) 1537
Psp5II RGGWCCY 1 cut(s) 1240
Psp6I CCWGG 2 cut(s) 1063, 1426
PspCI CACGTG 1 cut(s) 273
PspGI CCWGG 2 cut(s) 1063, 1426
PspN4I GGNNCC 6 cut(s) 412, 786, 979, 1241, 1402, 1674
PspPI GGNCC 6 cut(s) 48, 230, 672, 1240, 1663, 1730
PspPPI RGGWCCY 1 cut(s) 1240
PstI CTGCAG 1 cut(s) 1262
RsaI GTAC 3 cut(s) 510, 821, 1048
RsaNI GTAC 3 cut(s) 509, 820, 1047
RseI CAYNNNNRTG 2 cut(s) 1298, 1581
SacI GAGCTC 1 cut(s) 151
SapI GCTCTTC 2 cut(s) 535, 1312
SaqAI TTAA 4 cut(s) 662, 909, 1086, 1722
Sau3AI GATC 8 cut(s) 23, 171, 306, 610, 725, 830, 1514, 1633
Sau96I GGNCC 6 cut(s) 48, 230, 672, 1240, 1663, 1730
SchI GAGTC 2 cut(s) 1544, 1696
ScrFI CCNGG 2 cut(s) 1065, 1428
SduI GDGCHC 3 cut(s) 151, 413, 1307
SexAI ACCWGGT 1 cut(s) 1063
SfaNI GCATC 4 cut(s) 17, 286, 1372, 1633
SfcI CTRYAG 1 cut(s) 1258
SfuI TTCGAA 1 cut(s) 156
SinI GGWCC 5 cut(s) 230, 672, 1240, 1663, 1730
SmiMI CAYNNNNRTG 2 cut(s) 1298, 1581
Sse9I AATT 9 cut(s) 599, 716, 803, 808, 876, 957, 1175, 1251, 1719
SspI AATATT 2 cut(s) 1326, 1726
SspMI CTAG 4 cut(s) 80, 164, 1419, 1680
SstI GAGCTC 1 cut(s) 151
StyD4I CCNGG 2 cut(s) 1063, 1426
StyI CCWWGG 2 cut(s) 429, 1737
TaaI ACNGT 1 cut(s) 220
TaiI ACGT 3 cut(s) 275, 510, 1540
TaqI TCGA 8 cut(s) 96, 108, 156, 239, 254, 837, 1611, 1700
TaqII GACCGA 1 cut(s) 1651
TasI AATT 9 cut(s) 599, 716, 803, 808, 876, 957, 1175, 1251, 1719
TatI WGTACW 1 cut(s) 819
TauI GCSGC 4 cut(s) 8, 393, 404, 1673
TfiI GAWTC 3 cut(s) 362, 864, 1017
Tru1I TTAA 4 cut(s) 662, 909, 1086, 1722
Tru9I TTAA 4 cut(s) 662, 909, 1086, 1722
TseFI GTSAC 2 cut(s) 1267, 1291
TseI GCWGC 6 cut(s) 208, 211, 353, 404, 425, 1479
Tsp45I GTSAC 2 cut(s) 1267, 1291
TspDTI ATGAA 6 cut(s) 436, 455, 1188, 1328, 1557, 1737
TspGWI ACGGA 2 cut(s) 270, 1393
VpaK11BI GGWCC 5 cut(s) 230, 672, 1240, 1663, 1730
VspI ATTAAT 1 cut(s) 1722
XapI RAATTY 3 cut(s) 803, 1175, 1251
XmnI GAANNNNTTC 1 cut(s) 1139
XspI CTAG 4 cut(s) 80, 164, 1419, 1680
Zsp2I ATGCAT 1 cut(s) 251
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.