pycom04g09590

May be involved in modulation of pathogen defense and leaf cell death

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0000744
Physical Location & Seq
Reverse (-)
12302011 .. 12305286
3276 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g09590.6

Sequence Viewer

Length: 1332 bp
ATGAGTAAAACTAGAGTTGGCATGAAGATGTCAATATATGACCAAGTAAGCGTATCTTTTATTTCATGCAAATCAAGCATGATGTTAGTCTGGCAATTTTGTTTTTCTTTTATCTCAACAAAGTATGTTGTAATTTTTCTGCAGAACAAAGTTCCATTTGTGTCATTTTATGGAGTCCACCAGCTTCACATACTCATCTTCGTGCTAGCCGTCTTTCACGTGCTTTACTGCATAACAACCCTAGTGTTGGGCAGAGCAAAGATGAGAAGATGGAAAATATGGGAACTGGAAACAAAGACAGCTGACTACCAATTCTCACATGGTATGCTACCTTCAGTTTTCACACACCCGGAAAGATTCAGATTTGCCAGAGACACATCCTTTGGAAGAAGACACTTGAGCTTTTGGAGCCGCTCTCCCATTAGCTTATGGATTGTAAGTGTTTTCCGTAGTCTATTTGTAATTTGTACGATAAATAATACCCCAACTCTTTGGTTATTTTCTGTATTATTCCTCATGTCTAATACCCACGGCTCACGTTCATACCTGTGGCTCCCATTTGTTCCCCTAGTTGTAAGCACCAATCACCACCGTCCGATCCCTTTCCTCAAAGTCCCAGGGACAAAGCTGCAAGTTATAATAACGAAGATGGGACTGAAATTATCAGAAAGAGGTGAAGTGGTTAAGGGGACCCCGCTGGTTGAGCCTGGTGACCATCTCTTCTGGTTCAACAACCCTCGCCTGCTTCTTTACATCATCCACTTTGTTCTCTTCCAGGGTGAGTTTTATTTCTGTATTTATTTTTCACTCAAATCTTGCAGAAAAACTTATTTACTAGTCGTTATACAAATCCTGTGCTGCTATGTAACTCTTCCACTTTATGCGTTGGTAACACAGATGGGTTCAACTATGAAGCCTGTAATCTTCAATGACCGAGTGGCGACCGCACTCAAAAAATGGCACATCACAGCGAAGAAACACATAAAACACAACAAGAATGCAAGTCCAGCCAGTGCATCGGGCACTCCCTCGCATGCCATGTCCCCTGTTCATCTACTACGCAACTATCAATCCGAACAGGACACTGAAAGCGTTCGAACGTTACCAATAATGTCTTATTTTGATAACGAAGGTTCAGACTCACCGTTCCATCACCAAGACGATTTGACTTGGTCTCAGCAAGGTACAAACCTGCAGGGTCAGGAGGAAGAGATTAGTGCTCAAGGGCGTAACTCGGAGAGCAATGCTTTAGGCGGTTATGGTTCGATAATTCAACATGAGATTCAAGTTCAATCGGCGAAATTGACATTTGAGGAATCAGATAGGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mlo

Protein Analysis

444

Amino Acids

51.01

Weight (kDa)

9.49

Isoelectric Point (pI)

57.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 638
Acc36I ACCTGC 1 cut(s) 1200
AccBSI CCGCTC 1 cut(s) 414
AciI CCGC 4 cut(s) 412, 695, 945, 1254
AclI AACGTT 1 cut(s) 1100
AclWI GGATC 1 cut(s) 592
AcuI CTGAAG 1 cut(s) 318
AcvI CACGTG 1 cut(s) 220
AfaI GTAC 2 cut(s) 469, 1186
AfiI CCNNNNNNNGG 1 cut(s) 247
AgsI TTSAA 6 cut(s) 730, 906, 928, 1274, 1286, 1292
AhlI ACTAGT 1 cut(s) 835
AjnI CCWGG 3 cut(s) 616, 706, 774
AluBI AGCT 5 cut(s) 184, 302, 402, 426, 628
AluI AGCT 5 cut(s) 184, 302, 402, 426, 628
Alw21I GWGCWC 1 cut(s) 1222
Alw26I GTCTC 2 cut(s) 366, 1179
AlwI GGATC 1 cut(s) 592
ApeKI GCWGC 2 cut(s) 628, 858
ArsI GACNNNNNNTTYG 2 cut(s) 365, 397
Asp700I GAANNNNTTC 1 cut(s) 1092
AspS9I GGNCC 1 cut(s) 690
AsuC2I CCSGG 1 cut(s) 350
AsuHPI GGTGA 6 cut(s) 578, 686, 722, 791, 1134, 1145
AsuII TTCGAA 1 cut(s) 1096
AsuNHI GCTAGC 1 cut(s) 205
AvaII GGWCC 1 cut(s) 690
BaeGI GKGCMC 1 cut(s) 1025
BarI GAAGNNNNNNTAC 2 cut(s) 316, 348
BbrPI CACGTG 1 cut(s) 220
BbsI GAAGAC 1 cut(s) 397
Bbv12I GWGCWC 1 cut(s) 1222
BbvI GCAGC 2 cut(s) 615, 845
BccI CCATC 5 cut(s) 264, 643, 723, 892, 1158
BceAI ACGGC 2 cut(s) 194, 547
BciT130I CCWGG 3 cut(s) 618, 708, 776
BcnI CCSGG 1 cut(s) 350
BcoDI GTCTC 2 cut(s) 366, 1179
BcuI ACTAGT 1 cut(s) 835
BfaI CTAG 5 cut(s) 12, 206, 242, 569, 836
BfmI CTRYAG 2 cut(s) 140, 1193
BfuAI ACCTGC 1 cut(s) 1200
BisI GCNGC 3 cut(s) 412, 629, 859
BlsI GCNGC 3 cut(s) 413, 630, 860
Bme1390I CCNGG 4 cut(s) 350, 618, 708, 776
Bme18I GGWCC 1 cut(s) 690
BmgT120I GGNCC 1 cut(s) 690
BmiI GGNNCC 4 cut(s) 410, 554, 691, 692
BmrFI CCNGG 4 cut(s) 350, 618, 708, 776
BmsI GCATC 1 cut(s) 1025
BmtI GCTAGC 1 cut(s) 209
BpiI GAAGAC 1 cut(s) 397
BplI GAGNNNNNCTC 2 cut(s) 400, 432
Bpu14I TTCGAA 1 cut(s) 1096
BpuEI CTTGAG 2 cut(s) 418, 1206
BpuMI CCSGG 1 cut(s) 350
BsaAI YACGTR 1 cut(s) 220
BsaI GGTCTC 1 cut(s) 1179
BsaJI CCNNGG 4 cut(s) 529, 616, 617, 775
Bsc4I CCNNNNNNNGG 1 cut(s) 247
Bse1I ACTGG 2 cut(s) 291, 1011
Bse3DI GCAATG 1 cut(s) 1249
BseBI CCWGG 3 cut(s) 618, 708, 776
BseDI CCNNGG 4 cut(s) 529, 616, 617, 775
BseGI GGATG 2 cut(s) 377, 756
BseLI CCNNNNNNNGG 1 cut(s) 247
BseMI GCAATG 1 cut(s) 1249
BseMII CTCAG 1 cut(s) 1190
BseNI ACTGG 2 cut(s) 291, 1011
BseSI GKGCMC 1 cut(s) 1025
BseXI GCAGC 2 cut(s) 615, 845
Bsh1285I CGRYCG 1 cut(s) 945
BsiEI CGRYCG 1 cut(s) 945
BsiHKAI GWGCWC 1 cut(s) 1222
BsiSI CCGG 1 cut(s) 350
BslFI GGGAC 5 cut(s) 599, 634, 666, 703, 1027
BslI CCNNNNNNNGG 1 cut(s) 247
BsmAI GTCTC 2 cut(s) 366, 1179
BsmFI GGGAC 5 cut(s) 599, 634, 666, 703, 1027
BsmI GAATGC 1 cut(s) 1003
Bso31I GGTCTC 1 cut(s) 1179
Bsp119I TTCGAA 1 cut(s) 1096
Bsp1286I GDGCHC 2 cut(s) 1025, 1222
Bsp143I GATC 1 cut(s) 597
BspACI CCGC 4 cut(s) 412, 695, 945, 1254
BspCNI CTCAG 1 cut(s) 1189
BspLI GGNNCC 4 cut(s) 410, 554, 691, 692
BspMAI CTGCAG 2 cut(s) 144, 1197
BspMI ACCTGC 1 cut(s) 1200
BspOI GCTAGC 1 cut(s) 209
BspPI GGATC 1 cut(s) 592
BspT104I TTCGAA 1 cut(s) 1096
BspTNI GGTCTC 1 cut(s) 1179
BsrBI CCGCTC 1 cut(s) 414
BsrDI GCAATG 1 cut(s) 1249
BsrI ACTGG 2 cut(s) 291, 1011
BssECI CCNNGG 4 cut(s) 529, 616, 617, 775
BssMI GATC 1 cut(s) 597
Bst2UI CCWGG 3 cut(s) 618, 708, 776
Bst4CI ACNGT 2 cut(s) 593, 1146
Bst6I CTCTTC 4 cut(s) 725, 776, 876, 1203
BstBAI YACGTR 1 cut(s) 220
BstBI TTCGAA 1 cut(s) 1096
BstC8I GCNNGC 3 cut(s) 207, 743, 1035
BstDEI CTNAG 1 cut(s) 1176
BstDSI CCRYGG 1 cut(s) 529
BstEII GGTNACC 1 cut(s) 710
BstF5I GGATG 2 cut(s) 377, 756
BstKTI GATC 1 cut(s) 600
BstMAI GTCTC 2 cut(s) 366, 1179
BstMBI GATC 1 cut(s) 597
BstMCI CGRYCG 1 cut(s) 945
BstMWI GCNNNNNNNGC 4 cut(s) 75, 408, 703, 1007
BstNI CCWGG 3 cut(s) 618, 708, 776
BstNSI RCATGY 1 cut(s) 1037
BstPI GGTNACC 1 cut(s) 710
BstSCI CCNGG 4 cut(s) 348, 616, 706, 774
BstSFI CTRYAG 2 cut(s) 140, 1193
BstSLI GKGCMC 1 cut(s) 1025
BstV1I GCAGC 2 cut(s) 615, 845
BstV2I GAAGAC 1 cut(s) 397
BstXI CCANNNNNNTGG 1 cut(s) 492
BtgI CCRYGG 1 cut(s) 529
BtsCI GGATG 2 cut(s) 377, 756
BtsIMutI CAGTG 2 cut(s) 1018, 1083
BveI ACCTGC 1 cut(s) 1200
Cac8I GCNNGC 3 cut(s) 207, 743, 1035
Cfr13I GGNCC 1 cut(s) 690
Csp6I GTAC 2 cut(s) 468, 1185
CviAII CATG 8 cut(s) 22, 66, 79, 320, 517, 1034, 1039, 1277
CviQI GTAC 2 cut(s) 468, 1185
DdeI CTNAG 1 cut(s) 1176
DpnI GATC 1 cut(s) 599
DpnII GATC 1 cut(s) 597
Eam1104I CTCTTC 4 cut(s) 725, 776, 876, 1203
EarI CTCTTC 4 cut(s) 725, 776, 876, 1203
Eco31I GGTCTC 1 cut(s) 1179
Eco47I GGWCC 1 cut(s) 690
Eco57I CTGAAG 1 cut(s) 318
Eco72I CACGTG 1 cut(s) 220
Eco91I GGTNACC 1 cut(s) 710
EcoO109I RGGNCCY 1 cut(s) 690
EcoO65I GGTNACC 1 cut(s) 710
EcoRII CCWGG 3 cut(s) 616, 706, 774
FaeI CATG 8 cut(s) 25, 69, 82, 323, 520, 1037, 1042, 1280
FalI AAGNNNNNCTT 2 cut(s) 40, 72
FaqI GGGAC 5 cut(s) 599, 634, 666, 703, 1027
FatI CATG 8 cut(s) 21, 65, 78, 319, 516, 1033, 1038, 1276
FauI CCCGC 1 cut(s) 702
Fnu4HI GCNGC 3 cut(s) 412, 629, 859
FokI GGATG 2 cut(s) 364, 743
Fsp4HI GCNGC 3 cut(s) 412, 629, 859
FspBI CTAG 5 cut(s) 12, 206, 242, 569, 836
GluI GCNGC 3 cut(s) 412, 629, 859
HapII CCGG 1 cut(s) 350
Hin1II CATG 8 cut(s) 25, 69, 82, 323, 520, 1037, 1042, 1280
HinfI GANTC 5 cut(s) 174, 357, 1139, 1282, 1316
HpaII CCGG 1 cut(s) 350
HphI GGTGA 6 cut(s) 578, 686, 722, 791, 1134, 1145
Hpy166II GTNNAC 1 cut(s) 178
Hpy188I TCNGA 7 cut(s) 362, 597, 667, 1075, 1138, 1237, 1321
Hpy188III TCNNGA 1 cut(s) 1202
Hpy8I GTNNAC 1 cut(s) 178
HpyAV CCTTC 2 cut(s) 342, 1124
HpyCH4III ACNGT 2 cut(s) 593, 1146
HpyCH4IV ACGT 3 cut(s) 219, 538, 1100
HpyCH4V TGCA 8 cut(s) 69, 142, 231, 631, 819, 1001, 1016, 1195
HpyF10VI GCNNNNNNNGC 4 cut(s) 75, 408, 703, 1007
HpyF3I CTNAG 1 cut(s) 1176
HpySE526I ACGT 3 cut(s) 219, 538, 1100
Hsp92II CATG 8 cut(s) 25, 69, 82, 323, 520, 1037, 1042, 1280
KflI GGGWCCC 1 cut(s) 690
Kzo9I GATC 1 cut(s) 597
LmnI GCTCC 2 cut(s) 408, 558
Lsp1109I GCAGC 2 cut(s) 615, 845
LweI GCATC 1 cut(s) 1025
MaeI CTAG 5 cut(s) 12, 206, 242, 569, 836
MaeII ACGT 3 cut(s) 219, 538, 1100
MaeIII GTNAC 5 cut(s) 710, 865, 889, 1101, 1229
MalI GATC 1 cut(s) 599
MbiI CCGCTC 1 cut(s) 414
MboI GATC 1 cut(s) 597
MhlI GDGCHC 2 cut(s) 1025, 1222
MluCI AATT 7 cut(s) 95, 132, 311, 462, 659, 1269, 1301
MlyI GAGTC 2 cut(s) 183, 1133
MnlI CCTC 7 cut(s) 524, 617, 665, 747, 1039, 1198, 1306
MroXI GAANNNNTTC 1 cut(s) 1092
MseI TTAA 1 cut(s) 684
MslI CAYNNNNRTG 3 cut(s) 26, 200, 547
MspA1I CMGCKG 2 cut(s) 302, 697
MspI CCGG 1 cut(s) 350
MspR9I CCNGG 4 cut(s) 350, 618, 708, 776
Mva1269I GAATGC 1 cut(s) 1003
MvaI CCWGG 3 cut(s) 618, 708, 776
MwoI GCNNNNNNNGC 4 cut(s) 75, 408, 703, 1007
NciI CCSGG 1 cut(s) 350
NdeII GATC 1 cut(s) 597
NheI GCTAGC 1 cut(s) 205
NlaIII CATG 8 cut(s) 25, 69, 82, 323, 520, 1037, 1042, 1280
NlaIV GGNNCC 4 cut(s) 410, 554, 691, 692
NmuCI GTSAC 1 cut(s) 710
NspI RCATGY 1 cut(s) 1037
NspV TTCGAA 1 cut(s) 1096
PaeI GCATGC 1 cut(s) 1037
PasI CCCWGGG 1 cut(s) 617
PcsI WCGNNNNNNNCGW 1 cut(s) 207
PctI GAATGC 1 cut(s) 1003
PdmI GAANNNNTTC 1 cut(s) 1092
PfeI GAWTC 3 cut(s) 357, 1282, 1316
PflFI GACNNNGTC 1 cut(s) 1171
PkrI GCNGC 3 cut(s) 413, 630, 860
PleI GAGTC 2 cut(s) 182, 1133
PmaCI CACGTG 1 cut(s) 220
PmlI CACGTG 1 cut(s) 220
PpsI GAGTC 2 cut(s) 182, 1133
Ppu21I YACGTR 1 cut(s) 220
PpuMI RGGWCCY 1 cut(s) 690
PsiI TTATAA 1 cut(s) 638
Psp1406I AACGTT 1 cut(s) 1100
Psp5II RGGWCCY 1 cut(s) 690
Psp6I CCWGG 3 cut(s) 616, 706, 774
PspCI CACGTG 1 cut(s) 220
PspEI GGTNACC 1 cut(s) 710
PspGI CCWGG 3 cut(s) 616, 706, 774
PspN4I GGNNCC 4 cut(s) 410, 554, 691, 692
PspPI GGNCC 1 cut(s) 690
PspPPI RGGWCCY 1 cut(s) 690
PstI CTGCAG 2 cut(s) 144, 1197
PsyI GACNNNGTC 1 cut(s) 1171
PvuII CAGCTG 1 cut(s) 302
RsaI GTAC 2 cut(s) 469, 1186
RsaNI GTAC 2 cut(s) 468, 1185
RseI CAYNNNNRTG 3 cut(s) 26, 200, 547
SaqAI TTAA 1 cut(s) 684
SatI GCNGC 3 cut(s) 412, 629, 859
Sau3AI GATC 1 cut(s) 597
Sau96I GGNCC 1 cut(s) 690
SbfI CCTGCAGG 1 cut(s) 1197
SchI GAGTC 2 cut(s) 183, 1133
ScrFI CCNGG 4 cut(s) 350, 618, 708, 776
SdaI CCTGCAGG 1 cut(s) 1197
SduI GDGCHC 2 cut(s) 1025, 1222
SfaNI GCATC 1 cut(s) 1025
SfcI CTRYAG 2 cut(s) 140, 1193
SfuI TTCGAA 1 cut(s) 1096
SinI GGWCC 1 cut(s) 690
SmiMI CAYNNNNRTG 3 cut(s) 26, 200, 547
SmlI CTYRAG 2 cut(s) 397, 1221
SmoI CTYRAG 2 cut(s) 397, 1221
SpeI ACTAGT 1 cut(s) 835
SphI GCATGC 1 cut(s) 1037
Sse8387I CCTGCAGG 1 cut(s) 1197
Sse9I AATT 7 cut(s) 95, 132, 311, 462, 659, 1269, 1301
SsiI CCGC 4 cut(s) 412, 695, 945, 1254
SspMI CTAG 5 cut(s) 12, 206, 242, 569, 836
StyD4I CCNGG 4 cut(s) 348, 616, 706, 774
TaaI ACNGT 2 cut(s) 593, 1146
TaiI ACGT 3 cut(s) 222, 541, 1103
TaqI TCGA 2 cut(s) 1096, 1265
TaqII GACCGA 1 cut(s) 948
TasI AATT 7 cut(s) 95, 132, 311, 462, 659, 1269, 1301
TauI GCSGC 1 cut(s) 414
TfiI GAWTC 3 cut(s) 357, 1282, 1316
Tru1I TTAA 1 cut(s) 684
Tru9I TTAA 1 cut(s) 684
TscAI CASTG 2 cut(s) 1018, 1090
TseFI GTSAC 1 cut(s) 710
TseI GCWGC 2 cut(s) 628, 858
Tsp45I GTSAC 1 cut(s) 710
TspDTI ATGAA 5 cut(s) 38, 54, 531, 926, 1040
TspGWI ACGGA 1 cut(s) 437
TspRI CASTG 2 cut(s) 1018, 1090
Tth111I GACNNNGTC 1 cut(s) 1171
VpaK11BI GGWCC 1 cut(s) 690
XceI RCATGY 1 cut(s) 1037
XcmI CCANNNNNNNNNTGG 1 cut(s) 317
XmnI GAANNNNTTC 1 cut(s) 1092
XspI CTAG 5 cut(s) 12, 206, 242, 569, 836
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.