pycom12g12000

May be involved in modulation of pathogen defense and leaf cell death

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0000744
Physical Location & Seq
Reverse (-)
14445599 .. 14449703
4105 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g12000.3

Sequence Viewer

Length: 1593 bp
ATGGCAGGAGGAAAAAAAGGAAGATCGATCCTAGCTTTGGAGCAAACACCAACTTGGGCAGTTGCCGTTGTTTGTTTCGTTTTTGTTGTGATTTCGATCCTCATCGAATATTTCATTCATTTAATTGGAAAGTGGTTGAAAAGGAGAAACAAAAGAGCTCTCTATGAAGCACTTGAGAAGATCAAGTCGGAGCTTATGCTATTAGGGTTCCTATCCTTGCTTCTAACAGTAGGACAAGGCCCCATTTCAAATATTTGTATATCCAAGGCTGTTGGATCAACTTGGCATCCATGCAGTAAGAAGCAAGAGGTCAAATCAGACAAGAACGAAGATAAGAGTAGTGCTTCAGATGACAACGCCCGCAGAAGGCTTCTCTCAGCCTCGGATTCCAGTGGGGGTGGCCGACGTGTTTTAGCAGCTGCTGGATATGACAAATGTGCTGCCAAGAACAAAGTTCCATTTGTATCATATTATGGGATCCACCAGCTTCACATACTCATCTTTGTGCTAGCCGTCTTCCACGTGCTTTACTGCATAACAACCCTAGTTTTGGGCAGAGCAAAGATGAGAAAATGGAAAGCATGGGAACTGGAAACAAAAACAGCTGCTTACCAATTCTCACATGGTATGCTACCTCCCCATGCTTTATGCTACCATTATAGTATAATATGCATGACTAAATACTTCATCTTTCAAAAATATCGTCTGAGATTAACAACTTTCCACACTTGCAGCCCAACTATTTGGTTATTTGCTGTATTATTCCTCATGTCAAACACCCACGGCTCACGTTCTTATCTGTGGCTCCCATTTGTTCCCCTAGTTATGATCTTAATGGTAGGGACAAAGCTGCAAGTTATAATAACAAAGATGGGGCTCCAATTATCAGAAAGAGGTGAAGTGGTTAGGGGAACCCCACTGGTTGAGCCTGGTGACCATCTCTTCTGGTTCAATAACCCCCGACTCCTGCTTTATATCATCCACTTTGTTCTCTTTCAGGAAAAGGTGGTTTACATTTTTACCTTTTTACAGTATGAATTTGGCTTGAAGTCTTGCTTCCATGAGAAGTTGGAAGATGTCGTTTTAAGAATATCAATGGGGGTGATCATACAAATTCTGTGCAGCTATGTAACTCTTCCTCTTTATGCATTGGTAACACAGATGGGTTCAACTATGAAGCCTGTAATCTTCAACGACAGAGTGGTGACTGCACTCAAAAAATGGCACATCGCAGCGAAGAAGCACGTAAAACACAAGAATGCAAGTCCAGCCAGTGCACCAGGCACTCCTTTGCACTCCATGTCCCCTGTTCATCTACTACGCAACTATAAATATGAACAGGACATTGACAGCATTCGGACATCACCAAGAATGTCTTATTTTGATAACGAAGGTTCAGACTCACCGTTTCATCACCAAGACAATTTGACTTGGTCTCAGCAAGGTACGAACATGGGGGGTCAGAAGAAAGAGATTAGTGCTCATGGACCTAACTCAGAGAGCAATGCTTTAGGCGCTTATGGTTCGATAATTCAACATGAGATTCAAATTCACTCGGCAGCACTCACATTTGAGAAATCAGAAAGGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mlo

Protein Analysis

531

Amino Acids

60.05

Weight (kDa)

9.54

Isoelectric Point (pI)

47.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 860
AciI CCGC 1 cut(s) 361
AclWI GGATC 5 cut(s) 22, 91, 283, 472, 485
AcoI YGGCCR 1 cut(s) 400
AcsI RAATTY 3 cut(s) 1037, 1113, 1548
AcuI CTGAAG 1 cut(s) 330
AcvI CACGTG 1 cut(s) 523
AfaI GTAC 1 cut(s) 1447
AfiI CCNNNNNNNGG 3 cut(s) 37, 366, 550
AflIII ACRYGT 1 cut(s) 406
AgsI TTSAA 9 cut(s) 139, 249, 695, 952, 1048, 1170, 1192, 1535, 1547
AjiI CACGTC 1 cut(s) 407
AjnI CCWGG 2 cut(s) 928, 1279
AluBI AGCT 8 cut(s) 35, 158, 193, 419, 487, 605, 850, 1125
AluI AGCT 8 cut(s) 35, 158, 193, 419, 487, 605, 850, 1125
Alw21I GWGCWC 3 cut(s) 160, 1279, 1483
Alw26I GTCTC 1 cut(s) 1440
Alw44I GTGCAC 1 cut(s) 1275
AlwI GGATC 5 cut(s) 22, 91, 283, 472, 485
AlwNI CAGNNNCTG 1 cut(s) 422
AoxI GGCC 2 cut(s) 238, 400
ApaLI GTGCAC 1 cut(s) 1275
ApeKI GCWGC 9 cut(s) 416, 419, 440, 605, 732, 850, 1122, 1232, 1559
ApoI RAATTY 3 cut(s) 1037, 1113, 1548
ArsI GACNNNNNNTTYG 2 cut(s) 688, 720
AspLEI GCGC 1 cut(s) 1517
AspS9I GGNCC 2 cut(s) 239, 1487
AsuHPI GGTGA 7 cut(s) 908, 944, 1114, 1216, 1356, 1395, 1406
AsuNHI GCTAGC 1 cut(s) 508
AvaII GGWCC 1 cut(s) 1487
BaeGI GKGCMC 1 cut(s) 1279
BamHI GGATCC 1 cut(s) 477
BanII GRGCYC 2 cut(s) 160, 879
BbrPI CACGTG 1 cut(s) 523
BbsI GAAGAC 1 cut(s) 508
Bbv12I GWGCWC 3 cut(s) 160, 1279, 1483
BbvI GCAGC 9 cut(s) 406, 427, 428, 592, 744, 837, 1134, 1244, 1571
BccI CCATC 3 cut(s) 865, 945, 1156
BceAI ACGGC 3 cut(s) 50, 497, 799
BciT130I CCWGG 2 cut(s) 930, 1281
BclI TGATCA 1 cut(s) 1104
BcoDI GTCTC 1 cut(s) 1440
BfaI CTAG 4 cut(s) 32, 509, 545, 821
BfoI RGCGCY 1 cut(s) 1518
BisI GCNGC 9 cut(s) 417, 420, 441, 606, 733, 851, 1123, 1233, 1560
BlsI GCNGC 9 cut(s) 418, 421, 442, 607, 734, 852, 1124, 1234, 1561
Bme1390I CCNGG 2 cut(s) 930, 1281
Bme18I GGWCC 1 cut(s) 1487
BmgBI CACGTC 1 cut(s) 407
BmgT120I GGNCC 2 cut(s) 239, 1487
BmiI GGNNCC 6 cut(s) 209, 241, 479, 806, 878, 913
BmrFI CCNGG 2 cut(s) 930, 1281
BmsI GCATC 1 cut(s) 295
BmtI GCTAGC 1 cut(s) 512
BpiI GAAGAC 1 cut(s) 508
BpuEI CTTGAG 1 cut(s) 194
Bsa29I ATCGAT 1 cut(s) 26
BsaAI YACGTR 2 cut(s) 523, 1246
BsaBI GATNNNNATC 2 cut(s) 95, 101
BsaI GGTCTC 1 cut(s) 1440
BsaJI CCNNGG 3 cut(s) 264, 381, 781
Bsc4I CCNNNNNNNGG 3 cut(s) 37, 366, 550
Bse1I ACTGG 4 cut(s) 390, 594, 924, 1272
Bse3DI GCAATG 1 cut(s) 1510
Bse8I GATNNNNATC 2 cut(s) 95, 101
BseBI CCWGG 2 cut(s) 930, 1281
BseCI ATCGAT 1 cut(s) 26
BseDI CCNNGG 3 cut(s) 264, 381, 781
BseGI GGATG 2 cut(s) 286, 978
BseJI GATNNNNATC 2 cut(s) 95, 101
BseLI CCNNNNNNNGG 3 cut(s) 37, 366, 550
BseMI GCAATG 1 cut(s) 1510
BseMII CTCAG 4 cut(s) 390, 698, 1451, 1509
BseNI ACTGG 4 cut(s) 390, 594, 924, 1272
BseSI GKGCMC 1 cut(s) 1279
BseXI GCAGC 9 cut(s) 406, 427, 428, 592, 744, 837, 1134, 1244, 1571
BsgI GTGCAG 2 cut(s) 1141, 1194
BshFI GGCC 2 cut(s) 240, 402
BshVI ATCGAT 1 cut(s) 26
BsiHKAI GWGCWC 3 cut(s) 160, 1279, 1483
BslFI GGGAC 2 cut(s) 856, 1288
BslI CCNNNNNNNGG 3 cut(s) 37, 366, 550
BsmAI GTCTC 1 cut(s) 1440
BsmFI GGGAC 2 cut(s) 856, 1288
BsmI GAATGC 2 cut(s) 1264, 1353
BsnI GGCC 2 cut(s) 240, 402
Bso31I GGTCTC 1 cut(s) 1440
Bsp1286I GDGCHC 4 cut(s) 160, 879, 1279, 1483
Bsp143I GATC 8 cut(s) 23, 27, 96, 180, 275, 477, 828, 1104
BspACI CCGC 1 cut(s) 361
BspANI GGCC 2 cut(s) 240, 402
BspCNI CTCAG 4 cut(s) 389, 699, 1450, 1508
BspDI ATCGAT 1 cut(s) 26
BspLI GGNNCC 6 cut(s) 209, 241, 479, 806, 878, 913
BspOI GCTAGC 1 cut(s) 512
BspPI GGATC 5 cut(s) 22, 91, 283, 472, 485
BspTNI GGTCTC 1 cut(s) 1440
BsrDI GCAATG 1 cut(s) 1510
BsrI ACTGG 4 cut(s) 390, 594, 924, 1272
BssECI CCNNGG 3 cut(s) 264, 381, 781
BssMI GATC 8 cut(s) 23, 27, 96, 180, 275, 477, 828, 1104
BssT1I CCWWGG 1 cut(s) 264
Bst2UI CCWGG 2 cut(s) 930, 1281
Bst4CI ACNGT 3 cut(s) 229, 1032, 1407
Bst6I CTCTTC 2 cut(s) 947, 1140
BstBAI YACGTR 2 cut(s) 523, 1246
BstC8I GCNNGC 2 cut(s) 361, 510
BstDEI CTNAG 4 cut(s) 376, 707, 1437, 1495
BstDSI CCRYGG 1 cut(s) 781
BstEII GGTNACC 1 cut(s) 932
BstF5I GGATG 2 cut(s) 286, 978
BstH2I RGCGCY 1 cut(s) 1518
BstHHI GCGC 1 cut(s) 1517
BstKTI GATC 8 cut(s) 26, 30, 99, 183, 278, 480, 831, 1107
BstMAI GTCTC 1 cut(s) 1440
BstMBI GATC 8 cut(s) 23, 27, 96, 180, 275, 477, 828, 1104
BstMWI GCNNNNNNNGC 2 cut(s) 1268, 1514
BstNI CCWGG 2 cut(s) 930, 1281
BstPI GGTNACC 1 cut(s) 932
BstSCI CCNGG 2 cut(s) 928, 1279
BstSLI GKGCMC 1 cut(s) 1279
BstV1I GCAGC 9 cut(s) 406, 427, 428, 592, 744, 837, 1134, 1244, 1571
BstV2I GAAGAC 1 cut(s) 508
BstX2I RGATCY 1 cut(s) 477
BstXI CCANNNNNNTGG 1 cut(s) 744
BstYI RGATCY 1 cut(s) 477
Bsu15I ATCGAT 1 cut(s) 26
BsuRI GGCC 2 cut(s) 240, 402
BsuTUI ATCGAT 1 cut(s) 26
BtgI CCRYGG 1 cut(s) 781
BtgZI GCGATG 1 cut(s) 1213
BtrI CACGTC 1 cut(s) 407
BtsCI GGATG 2 cut(s) 286, 978
BtsIMutI CAGTG 3 cut(s) 397, 917, 1279
Cac8I GCNNGC 2 cut(s) 361, 510
CaiI CAGNNNCTG 1 cut(s) 422
CfoI GCGC 1 cut(s) 1517
Cfr13I GGNCC 2 cut(s) 239, 1487
ClaI ATCGAT 1 cut(s) 26
Csp6I GTAC 1 cut(s) 1446
CviQI GTAC 1 cut(s) 1446
DdeI CTNAG 4 cut(s) 376, 707, 1437, 1495
DpnI GATC 8 cut(s) 25, 29, 98, 182, 277, 479, 830, 1106
DpnII GATC 8 cut(s) 23, 27, 96, 180, 275, 477, 828, 1104
EaeI YGGCCR 1 cut(s) 400
Eam1104I CTCTTC 2 cut(s) 947, 1140
EarI CTCTTC 2 cut(s) 947, 1140
Ecl136II GAGCTC 1 cut(s) 158
Eco130I CCWWGG 1 cut(s) 264
Eco24I GRGCYC 2 cut(s) 160, 879
Eco31I GGTCTC 1 cut(s) 1440
Eco47I GGWCC 1 cut(s) 1487
Eco53kI GAGCTC 1 cut(s) 158
Eco57I CTGAAG 1 cut(s) 330
Eco72I CACGTG 1 cut(s) 523
Eco91I GGTNACC 1 cut(s) 932
EcoICRI GAGCTC 1 cut(s) 158
EcoO109I RGGNCCY 1 cut(s) 239
EcoO65I GGTNACC 1 cut(s) 932
EcoRII CCWGG 2 cut(s) 928, 1279
EcoT14I CCWWGG 1 cut(s) 264
EcoT22I ATGCAT 2 cut(s) 674, 1150
EcoT38I GRGCYC 2 cut(s) 160, 879
ErhI CCWWGG 1 cut(s) 264
FalI AAGNNNNNCTT 4 cut(s) 1040, 1072, 1360, 1392
FaqI GGGAC 2 cut(s) 856, 1288
FauI CCCGC 1 cut(s) 368
FbaI TGATCA 1 cut(s) 1104
Fnu4HI GCNGC 9 cut(s) 417, 420, 441, 606, 733, 851, 1123, 1233, 1560
FokI GGATG 2 cut(s) 273, 965
FriOI GRGCYC 2 cut(s) 160, 879
Fsp4HI GCNGC 9 cut(s) 417, 420, 441, 606, 733, 851, 1123, 1233, 1560
FspBI CTAG 4 cut(s) 32, 509, 545, 821
GlaI GCGC 1 cut(s) 1516
GluI GCNGC 9 cut(s) 417, 420, 441, 606, 733, 851, 1123, 1233, 1560
HaeII RGCGCY 1 cut(s) 1518
HaeIII GGCC 2 cut(s) 240, 402
HhaI GCGC 1 cut(s) 1517
Hin6I GCGC 1 cut(s) 1515
HinP1I GCGC 1 cut(s) 1515
HinfI GANTC 4 cut(s) 386, 963, 1400, 1543
HphI GGTGA 7 cut(s) 908, 944, 1114, 1216, 1356, 1395, 1406
Hpy166II GTNNAC 2 cut(s) 1012, 1277
Hpy188III TCNNGA 1 cut(s) 998
Hpy8I GTNNAC 2 cut(s) 1012, 1277
Hpy99I CGWCG 1 cut(s) 408
HpyAV CCTTC 2 cut(s) 360, 1385
HpyCH4III ACNGT 3 cut(s) 229, 1032, 1407
HpyCH4IV ACGT 4 cut(s) 406, 522, 790, 1245
HpyF10VI GCNNNNNNNGC 2 cut(s) 1268, 1514
HpyF3I CTNAG 4 cut(s) 376, 707, 1437, 1495
HpySE526I ACGT 4 cut(s) 406, 522, 790, 1245
HspAI GCGC 1 cut(s) 1515
Ksp22I TGATCA 1 cut(s) 1104
Kzo9I GATC 8 cut(s) 23, 27, 96, 180, 275, 477, 828, 1104
LmnI GCTCC 4 cut(s) 40, 190, 810, 882
Lsp1109I GCAGC 9 cut(s) 406, 427, 428, 592, 744, 837, 1134, 1244, 1571
LweI GCATC 1 cut(s) 295
MaeI CTAG 4 cut(s) 32, 509, 545, 821
MaeII ACGT 4 cut(s) 406, 522, 790, 1245
MaeIII GTNAC 4 cut(s) 932, 1129, 1153, 1204
MalI GATC 8 cut(s) 25, 29, 98, 182, 277, 479, 830, 1106
MboI GATC 8 cut(s) 23, 27, 96, 180, 275, 477, 828, 1104
MflI RGATCY 1 cut(s) 477
MhlI GDGCHC 4 cut(s) 160, 879, 1279, 1483
MluCI AATT 8 cut(s) 123, 614, 881, 1037, 1113, 1423, 1530, 1548
MlyI GAGTC 2 cut(s) 957, 1394
MmeI TCCRAC 3 cut(s) 168, 253, 1050
MnlI CCTC 7 cut(s) 110, 301, 391, 645, 776, 887, 1149
Mph1103I ATGCAT 2 cut(s) 674, 1150
MseI TTAA 4 cut(s) 122, 713, 833, 1085
MslI CAYNNNNRTG 2 cut(s) 503, 1257
MspA1I CMGCKG 2 cut(s) 419, 605
MspR9I CCNGG 2 cut(s) 930, 1281
Mva1269I GAATGC 2 cut(s) 1264, 1353
MvaI CCWGG 2 cut(s) 930, 1281
MwoI GCNNNNNNNGC 2 cut(s) 1268, 1514
NdeII GATC 8 cut(s) 23, 27, 96, 180, 275, 477, 828, 1104
NheI GCTAGC 1 cut(s) 508
NlaIV GGNNCC 6 cut(s) 209, 241, 479, 806, 878, 913
NmeAIII GCCGAG 1 cut(s) 1535
NmuCI GTSAC 2 cut(s) 932, 1204
NsiI ATGCAT 2 cut(s) 674, 1150
PctI GAATGC 2 cut(s) 1264, 1353
PfeI GAWTC 2 cut(s) 386, 1543
PflFI GACNNNGTC 1 cut(s) 1432
PkrI GCNGC 9 cut(s) 418, 421, 442, 607, 734, 852, 1124, 1234, 1561
PleI GAGTC 2 cut(s) 957, 1394
PmaCI CACGTG 1 cut(s) 523
PmlI CACGTG 1 cut(s) 523
PpsI GAGTC 2 cut(s) 957, 1394
Ppu21I YACGTR 2 cut(s) 523, 1246
PsiI TTATAA 1 cut(s) 860
Psp124BI GAGCTC 1 cut(s) 160
Psp6I CCWGG 2 cut(s) 928, 1279
PspCI CACGTG 1 cut(s) 523
PspEI GGTNACC 1 cut(s) 932
PspGI CCWGG 2 cut(s) 928, 1279
PspN4I GGNNCC 6 cut(s) 209, 241, 479, 806, 878, 913
PspPI GGNCC 2 cut(s) 239, 1487
PstNI CAGNNNCTG 1 cut(s) 422
PsuI RGATCY 1 cut(s) 477
PsyI GACNNNGTC 1 cut(s) 1432
PvuII CAGCTG 2 cut(s) 419, 605
RsaI GTAC 1 cut(s) 1447
RsaNI GTAC 1 cut(s) 1446
RseI CAYNNNNRTG 2 cut(s) 503, 1257
SacI GAGCTC 1 cut(s) 160
SaqAI TTAA 4 cut(s) 122, 713, 833, 1085
SatI GCNGC 9 cut(s) 417, 420, 441, 606, 733, 851, 1123, 1233, 1560
Sau3AI GATC 8 cut(s) 23, 27, 96, 180, 275, 477, 828, 1104
Sau96I GGNCC 2 cut(s) 239, 1487
SchI GAGTC 2 cut(s) 957, 1394
ScrFI CCNGG 2 cut(s) 930, 1281
SduI GDGCHC 4 cut(s) 160, 879, 1279, 1483
SfaNI GCATC 1 cut(s) 295
SinI GGWCC 1 cut(s) 1487
SmiMI CAYNNNNRTG 2 cut(s) 503, 1257
SmlI CTYRAG 1 cut(s) 173
SmoI CTYRAG 1 cut(s) 173
Sse9I AATT 8 cut(s) 123, 614, 881, 1037, 1113, 1423, 1530, 1548
SsiI CCGC 1 cut(s) 361
SspI AATATT 2 cut(s) 110, 253
SspMI CTAG 4 cut(s) 32, 509, 545, 821
SstI GAGCTC 1 cut(s) 160
StyD4I CCNGG 2 cut(s) 928, 1279
StyI CCWWGG 1 cut(s) 264
TaaI ACNGT 3 cut(s) 229, 1032, 1407
TaiI ACGT 4 cut(s) 409, 525, 793, 1248
TaqI TCGA 4 cut(s) 26, 95, 105, 1526
TasI AATT 8 cut(s) 123, 614, 881, 1037, 1113, 1423, 1530, 1548
TfiI GAWTC 2 cut(s) 386, 1543
Tru1I TTAA 4 cut(s) 122, 713, 833, 1085
Tru9I TTAA 4 cut(s) 122, 713, 833, 1085
TscAI CASTG 3 cut(s) 397, 924, 1279
TseFI GTSAC 2 cut(s) 932, 1204
TseI GCWGC 9 cut(s) 416, 419, 440, 605, 732, 850, 1122, 1232, 1559
Tsp45I GTSAC 2 cut(s) 932, 1204
TspDTI ATGAA 9 cut(s) 103, 107, 180, 676, 1050, 1190, 1301, 1350, 1400
TspRI CASTG 3 cut(s) 397, 924, 1279
Tth111I GACNNNGTC 1 cut(s) 1432
VneI GTGCAC 1 cut(s) 1275
VpaK11BI GGWCC 1 cut(s) 1487
XapI RAATTY 3 cut(s) 1037, 1113, 1548
XcmI CCANNNNNNNNNTGG 1 cut(s) 620
XspI CTAG 4 cut(s) 32, 509, 545, 821
Zsp2I ATGCAT 2 cut(s) 674, 1150
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.