May be involved in modulation of pathogen defense and leaf cell death
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 929 |
| Acc16I | TGCGCA | 1 cut(s) | 1424 |
| Acc36I | ACCTGC | 1 cut(s) | 357 |
| AccB1I | GGYRCC | 3 cut(s) | 751, 1013, 1636 |
| AccBSI | CCGCTC | 1 cut(s) | 1435 |
| AciI | CCGC | 3 cut(s) | 1433, 1462, 1640 |
| AclWI | GGATC | 4 cut(s) | 338, 578, 1202, 1608 |
| AcoI | YGGCCR | 2 cut(s) | 3, 1411 |
| AcsI | RAATTY | 1 cut(s) | 638 |
| AcuI | CTGAAG | 4 cut(s) | 337, 661, 1340, 1551 |
| AcyI | GRCGYC | 1 cut(s) | 1014 |
| AdeI | CACNNNGTG | 1 cut(s) | 1420 |
| AfaI | GTAC | 5 cut(s) | 575, 788, 1135, 1396, 1654 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 940, 1415 |
| AjnI | CCWGG | 1 cut(s) | 1642 |
| AluBI | AGCT | 6 cut(s) | 149, 184, 685, 1114, 1154, 1326 |
| AluI | AGCT | 6 cut(s) | 149, 184, 685, 1114, 1154, 1326 |
| Alw21I | GWGCWC | 3 cut(s) | 151, 186, 486 |
| Alw26I | GTCTC | 2 cut(s) | 545, 622 |
| AlwI | GGATC | 4 cut(s) | 338, 578, 1202, 1608 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 347 |
| AoxI | GGCC | 4 cut(s) | 3, 267, 1116, 1411 |
| ApeKI | GCWGC | 3 cut(s) | 682, 1444, 1464 |
| ApoI | RAATTY | 1 cut(s) | 638 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 602, 634 |
| AspLEI | GCGC | 4 cut(s) | 366, 1016, 1173, 1425 |
| AspS9I | GGNCC | 2 cut(s) | 230, 267 |
| AsuC2I | CCSGG | 3 cut(s) | 1032, 1401, 1410 |
| AsuHPI | GGTGA | 3 cut(s) | 431, 639, 1031 |
| AsuNHI | GCTAGC | 1 cut(s) | 466 |
| AvaII | GGWCC | 1 cut(s) | 230 |
| AxyI | CCTNAGG | 1 cut(s) | 722 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 557, 590 |
| BanI | GGYRCC | 3 cut(s) | 751, 1013, 1636 |
| BanII | GRGCYC | 2 cut(s) | 151, 186 |
| Bbv12I | GWGCWC | 3 cut(s) | 151, 186, 486 |
| BbvCI | CCTCAGC | 1 cut(s) | 980 |
| BbvI | GCAGC | 3 cut(s) | 694, 1431, 1476 |
| BccI | CCATC | 6 cut(s) | 882, 931, 967, 1261, 1446, 1469 |
| BceAI | ACGGC | 1 cut(s) | 1432 |
| BciT130I | CCWGG | 1 cut(s) | 1644 |
| BclI | TGATCA | 1 cut(s) | 1188 |
| BcnI | CCSGG | 3 cut(s) | 1032, 1401, 1410 |
| BcoDI | GTCTC | 2 cut(s) | 545, 622 |
| BfaI | CTAG | 5 cut(s) | 29, 152, 467, 702, 1256 |
| BfmI | CTRYAG | 2 cut(s) | 283, 855 |
| BfoI | RGCGCY | 1 cut(s) | 1017 |
| BfuAI | ACCTGC | 1 cut(s) | 357 |
| BisI | GCNGC | 3 cut(s) | 683, 1445, 1465 |
| BlsI | GCNGC | 3 cut(s) | 684, 1446, 1466 |
| Bme1390I | CCNGG | 4 cut(s) | 1032, 1401, 1410, 1644 |
| Bme18I | GGWCC | 1 cut(s) | 230 |
| BmgT120I | GGNCC | 2 cut(s) | 230, 267 |
| BmiI | GGNNCC | 4 cut(s) | 268, 753, 1015, 1638 |
| BmrFI | CCNGG | 4 cut(s) | 1032, 1401, 1410, 1644 |
| BmsI | GCATC | 4 cut(s) | 286, 501, 1459, 1508 |
| BmtI | GCTAGC | 1 cut(s) | 470 |
| Bpu10I | CCTNAGC | 1 cut(s) | 980 |
| BpuEI | CTTGAG | 2 cut(s) | 185, 1375 |
| BpuMI | CCSGG | 3 cut(s) | 1032, 1401, 1410 |
| BsaBI | GATNNNNATC | 2 cut(s) | 202, 1193 |
| BsaHI | GRCGYC | 1 cut(s) | 1014 |
| BsaJI | CCNNGG | 2 cut(s) | 1414, 1643 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 940, 1415 |
| Bse1I | ACTGG | 2 cut(s) | 691, 1602 |
| Bse21I | CCTNAGG | 1 cut(s) | 722 |
| Bse8I | GATNNNNATC | 2 cut(s) | 202, 1193 |
| BseBI | CCWGG | 1 cut(s) | 1644 |
| BseDI | CCNNGG | 2 cut(s) | 1414, 1643 |
| BseGI | GGATG | 5 cut(s) | 277, 492, 893, 1006, 1080 |
| BseJI | GATNNNNATC | 2 cut(s) | 202, 1193 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 940, 1415 |
| BseMII | CTCAG | 8 cut(s) | 171, 354, 713, 971, 1372, 1585, 1670, 1674 |
| BseNI | ACTGG | 2 cut(s) | 691, 1602 |
| BseXI | GCAGC | 3 cut(s) | 694, 1431, 1476 |
| BsgI | GTGCAG | 2 cut(s) | 431, 1430 |
| BshFI | GGCC | 4 cut(s) | 5, 269, 1118, 1413 |
| BshNI | GGYRCC | 3 cut(s) | 751, 1013, 1636 |
| BsiHKAI | GWGCWC | 3 cut(s) | 151, 186, 486 |
| BsiSI | CCGG | 4 cut(s) | 1032, 1400, 1410, 1634 |
| BslFI | GGGAC | 2 cut(s) | 623, 1414 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 940, 1415 |
| BsmAI | GTCTC | 2 cut(s) | 545, 622 |
| BsmFI | GGGAC | 2 cut(s) | 623, 1414 |
| BsmI | GAATGC | 1 cut(s) | 1108 |
| BsnI | GGCC | 4 cut(s) | 5, 269, 1118, 1413 |
| Bsp1286I | GDGCHC | 3 cut(s) | 151, 186, 486 |
| Bsp143I | GATC | 8 cut(s) | 343, 583, 797, 831, 1036, 1188, 1194, 1600 |
| BspACI | CCGC | 3 cut(s) | 1433, 1462, 1640 |
| BspANI | GGCC | 4 cut(s) | 5, 269, 1118, 1413 |
| BspCNI | CTCAG | 8 cut(s) | 172, 353, 714, 972, 1371, 1584, 1669, 1675 |
| BspLI | GGNNCC | 4 cut(s) | 268, 753, 1015, 1638 |
| BspMAI | CTGCAG | 2 cut(s) | 287, 859 |
| BspMI | ACCTGC | 1 cut(s) | 357 |
| BspOI | GCTAGC | 1 cut(s) | 470 |
| BspPI | GGATC | 4 cut(s) | 338, 578, 1202, 1608 |
| BspQI | GCTCTTC | 2 cut(s) | 692, 1316 |
| BspT107I | GGYRCC | 3 cut(s) | 751, 1013, 1636 |
| BsrBI | CCGCTC | 1 cut(s) | 1435 |
| BsrI | ACTGG | 2 cut(s) | 691, 1602 |
| BssECI | CCNNGG | 2 cut(s) | 1414, 1643 |
| BssMI | GATC | 8 cut(s) | 343, 583, 797, 831, 1036, 1188, 1194, 1600 |
| BssNI | GRCGYC | 1 cut(s) | 1014 |
| Bst2UI | CCWGG | 1 cut(s) | 1644 |
| Bst4CI | ACNGT | 3 cut(s) | 220, 761, 1291 |
| Bst6I | CTCTTC | 6 cut(s) | 692, 801, 1049, 1299, 1316, 1578 |
| BstACI | GRCGYC | 1 cut(s) | 1014 |
| BstC8I | GCNNGC | 5 cut(s) | 78, 468, 1116, 1328, 1421 |
| BstDEI | CTNAG | 9 cut(s) | 11, 180, 340, 722, 980, 1358, 1571, 1656, 1683 |
| BstDSI | CCRYGG | 1 cut(s) | 1414 |
| BstEII | GGTNACC | 1 cut(s) | 645 |
| BstF5I | GGATG | 5 cut(s) | 277, 492, 893, 1006, 1080 |
| BstH2I | RGCGCY | 1 cut(s) | 1017 |
| BstHHI | GCGC | 4 cut(s) | 366, 1016, 1173, 1425 |
| BstKTI | GATC | 8 cut(s) | 346, 586, 800, 834, 1039, 1191, 1197, 1603 |
| BstMAI | GTCTC | 2 cut(s) | 545, 622 |
| BstMBI | GATC | 8 cut(s) | 343, 583, 797, 831, 1036, 1188, 1194, 1600 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 749, 868, 1124, 1441, 1450 |
| BstNI | CCWGG | 1 cut(s) | 1644 |
| BstNSI | RCATGY | 1 cut(s) | 1429 |
| BstPI | GGTNACC | 1 cut(s) | 645 |
| BstSCI | CCNGG | 4 cut(s) | 1030, 1399, 1408, 1642 |
| BstSFI | CTRYAG | 2 cut(s) | 283, 855 |
| BstV1I | GCAGC | 3 cut(s) | 694, 1431, 1476 |
| BstX2I | RGATCY | 2 cut(s) | 343, 1194 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 846 |
| BstYI | RGATCY | 2 cut(s) | 343, 1194 |
| Bsu36I | CCTNAGG | 1 cut(s) | 722 |
| BsuRI | GGCC | 4 cut(s) | 5, 269, 1118, 1413 |
| BtgI | CCRYGG | 1 cut(s) | 1414 |
| BtgZI | GCGATG | 1 cut(s) | 1482 |
| BtsCI | GGATG | 5 cut(s) | 277, 492, 893, 1006, 1080 |
| BtsI | GCAGTG | 1 cut(s) | 864 |
| BtsIMutI | CAGTG | 4 cut(s) | 766, 864, 1316, 1579 |
| BveI | ACCTGC | 1 cut(s) | 357 |
| Cac8I | GCNNGC | 5 cut(s) | 78, 468, 1116, 1328, 1421 |
| CaiI | CAGNNNCTG | 1 cut(s) | 347 |
| CfoI | GCGC | 4 cut(s) | 366, 1016, 1173, 1425 |
| Cfr13I | GGNCC | 2 cut(s) | 230, 267 |
| Csp6I | GTAC | 5 cut(s) | 574, 787, 1134, 1395, 1653 |
| CviQI | GTAC | 5 cut(s) | 574, 787, 1134, 1395, 1653 |
| DdeI | CTNAG | 9 cut(s) | 11, 180, 340, 722, 980, 1358, 1571, 1656, 1683 |
| DinI | GGCGCC | 1 cut(s) | 1015 |
| DpnI | GATC | 8 cut(s) | 345, 585, 799, 833, 1038, 1190, 1196, 1602 |
| DpnII | GATC | 8 cut(s) | 343, 583, 797, 831, 1036, 1188, 1194, 1600 |
| DraIII | CACNNNGTG | 1 cut(s) | 1420 |
| EaeI | YGGCCR | 2 cut(s) | 3, 1411 |
| Eam1104I | CTCTTC | 6 cut(s) | 692, 801, 1049, 1299, 1316, 1578 |
| EarI | CTCTTC | 6 cut(s) | 692, 801, 1049, 1299, 1316, 1578 |
| Ecl136II | GAGCTC | 2 cut(s) | 149, 184 |
| Eco24I | GRGCYC | 2 cut(s) | 151, 186 |
| Eco32I | GATATC | 1 cut(s) | 1183 |
| Eco47I | GGWCC | 1 cut(s) | 230 |
| Eco53kI | GAGCTC | 2 cut(s) | 149, 184 |
| Eco57I | CTGAAG | 4 cut(s) | 337, 661, 1340, 1551 |
| Eco81I | CCTNAGG | 1 cut(s) | 722 |
| Eco91I | GGTNACC | 1 cut(s) | 645 |
| EcoICRI | GAGCTC | 2 cut(s) | 149, 184 |
| EcoO109I | RGGNCCY | 2 cut(s) | 230, 267 |
| EcoO65I | GGTNACC | 1 cut(s) | 645 |
| EcoRII | CCWGG | 1 cut(s) | 1642 |
| EcoRV | GATATC | 1 cut(s) | 1183 |
| EcoT22I | ATGCAT | 2 cut(s) | 251, 1108 |
| EcoT38I | GRGCYC | 2 cut(s) | 151, 186 |
| EgeI | GGCGCC | 1 cut(s) | 1015 |
| EheI | GGCGCC | 1 cut(s) | 1015 |
| FaqI | GGGAC | 2 cut(s) | 623, 1414 |
| FauI | CCCGC | 1 cut(s) | 1440 |
| FauNDI | CATATG | 2 cut(s) | 883, 1138 |
| FbaI | TGATCA | 1 cut(s) | 1188 |
| Fnu4HI | GCNGC | 3 cut(s) | 683, 1445, 1465 |
| FokI | GGATG | 5 cut(s) | 264, 479, 900, 1013, 1067 |
| FriOI | GRGCYC | 2 cut(s) | 151, 186 |
| Fsp4HI | GCNGC | 3 cut(s) | 683, 1445, 1465 |
| FspAI | RTGCGCAY | 1 cut(s) | 1424 |
| FspBI | CTAG | 5 cut(s) | 29, 152, 467, 702, 1256 |
| FspI | TGCGCA | 1 cut(s) | 1424 |
| GlaI | GCGC | 4 cut(s) | 365, 1015, 1172, 1424 |
| GluI | GCNGC | 3 cut(s) | 683, 1445, 1465 |
| HaeII | RGCGCY | 1 cut(s) | 1017 |
| HaeIII | GGCC | 4 cut(s) | 5, 269, 1118, 1413 |
| HapII | CCGG | 4 cut(s) | 1032, 1400, 1410, 1634 |
| HhaI | GCGC | 4 cut(s) | 366, 1016, 1173, 1425 |
| Hin1I | GRCGYC | 1 cut(s) | 1014 |
| Hin6I | GCGC | 4 cut(s) | 364, 1014, 1171, 1423 |
| HinP1I | GCGC | 4 cut(s) | 364, 1014, 1171, 1423 |
| HincII | GTYRAC | 3 cut(s) | 129, 504, 1669 |
| HindII | GTYRAC | 3 cut(s) | 129, 504, 1669 |
| HinfI | GANTC | 6 cut(s) | 299, 436, 985, 1237, 1569, 1648 |
| HpaI | GTTAAC | 2 cut(s) | 129, 1669 |
| HpaII | CCGG | 4 cut(s) | 1032, 1400, 1410, 1634 |
| HphI | GGTGA | 3 cut(s) | 431, 639, 1031 |
| Hpy166II | GTNNAC | 3 cut(s) | 129, 504, 1669 |
| Hpy188I | TCNGA | 8 cut(s) | 181, 241, 310, 343, 400, 783, 1101, 1684 |
| Hpy8I | GTNNAC | 3 cut(s) | 129, 504, 1669 |
| HpyAV | CCTTC | 4 cut(s) | 529, 1003, 1129, 1526 |
| HpyCH4III | ACNGT | 3 cut(s) | 220, 761, 1291 |
| HpyCH4IV | ACGT | 2 cut(s) | 100, 500 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 749, 868, 1124, 1441, 1450 |
| HpyF3I | CTNAG | 9 cut(s) | 11, 180, 340, 722, 980, 1358, 1571, 1656, 1683 |
| HpySE526I | ACGT | 2 cut(s) | 100, 500 |
| Hsp92I | GRCGYC | 1 cut(s) | 1014 |
| HspAI | GCGC | 4 cut(s) | 364, 1014, 1171, 1423 |
| KasI | GGCGCC | 1 cut(s) | 1013 |
| Ksp22I | TGATCA | 1 cut(s) | 1188 |
| KspAI | GTTAAC | 2 cut(s) | 129, 1669 |
| Kzo9I | GATC | 8 cut(s) | 343, 583, 797, 831, 1036, 1188, 1194, 1600 |
| LguI | GCTCTTC | 2 cut(s) | 692, 1316 |
| LmnI | GCTCC | 2 cut(s) | 216, 1440 |
| Lsp1109I | GCAGC | 3 cut(s) | 694, 1431, 1476 |
| LweI | GCATC | 4 cut(s) | 286, 501, 1459, 1508 |
| MaeI | CTAG | 5 cut(s) | 29, 152, 467, 702, 1256 |
| MaeII | ACGT | 2 cut(s) | 100, 500 |
| MaeIII | GTNAC | 6 cut(s) | 356, 645, 697, 1234, 1258, 1574 |
| MalI | GATC | 8 cut(s) | 345, 585, 799, 833, 1038, 1190, 1196, 1602 |
| MbiI | CCGCTC | 1 cut(s) | 1435 |
| MboI | GATC | 8 cut(s) | 343, 583, 797, 831, 1036, 1188, 1194, 1600 |
| MfeI | CAATTG | 2 cut(s) | 561, 1625 |
| MflI | RGATCY | 2 cut(s) | 343, 1194 |
| MhlI | GDGCHC | 3 cut(s) | 151, 186, 486 |
| MluCI | AATT | 6 cut(s) | 561, 638, 840, 1217, 1511, 1625 |
| Mly113I | GGCGCC | 1 cut(s) | 1014 |
| MlyI | GAGTC | 3 cut(s) | 308, 1231, 1563 |
| MmeI | TCCRAC | 1 cut(s) | 900 |
| Mph1103I | ATGCAT | 2 cut(s) | 251, 1108 |
| MseI | TTAA | 4 cut(s) | 128, 174, 1514, 1668 |
| MslI | CAYNNNNRTG | 2 cut(s) | 43, 886 |
| MspA1I | CMGCKG | 1 cut(s) | 1114 |
| MspI | CCGG | 4 cut(s) | 1032, 1400, 1410, 1634 |
| MspR9I | CCNGG | 4 cut(s) | 1032, 1401, 1410, 1644 |
| MunI | CAATTG | 2 cut(s) | 561, 1625 |
| Mva1269I | GAATGC | 1 cut(s) | 1108 |
| MvaI | CCWGG | 1 cut(s) | 1644 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 749, 868, 1124, 1441, 1450 |
| NarI | GGCGCC | 1 cut(s) | 1014 |
| NciI | CCSGG | 3 cut(s) | 1032, 1401, 1410 |
| NdeI | CATATG | 2 cut(s) | 883, 1138 |
| NdeII | GATC | 8 cut(s) | 343, 583, 797, 831, 1036, 1188, 1194, 1600 |
| NheI | GCTAGC | 1 cut(s) | 466 |
| NlaIV | GGNNCC | 4 cut(s) | 268, 753, 1015, 1638 |
| NmuCI | GTSAC | 4 cut(s) | 645, 1234, 1258, 1574 |
| NsbI | TGCGCA | 1 cut(s) | 1424 |
| NsiI | ATGCAT | 2 cut(s) | 251, 1108 |
| NspI | RCATGY | 1 cut(s) | 1429 |
| PciSI | GCTCTTC | 2 cut(s) | 692, 1316 |
| PctI | GAATGC | 1 cut(s) | 1108 |
| PfeI | GAWTC | 3 cut(s) | 436, 985, 1648 |
| PflFI | GACNNNGTC | 1 cut(s) | 556 |
| PkrI | GCNGC | 3 cut(s) | 684, 1446, 1466 |
| PleI | GAGTC | 3 cut(s) | 307, 1231, 1563 |
| PluTI | GGCGCC | 1 cut(s) | 1017 |
| PpsI | GAGTC | 3 cut(s) | 307, 1231, 1563 |
| PpuMI | RGGWCCY | 1 cut(s) | 230 |
| PsiI | TTATAA | 1 cut(s) | 929 |
| Psp124BI | GAGCTC | 2 cut(s) | 151, 186 |
| Psp5II | RGGWCCY | 1 cut(s) | 230 |
| Psp6I | CCWGG | 1 cut(s) | 1642 |
| PspEI | GGTNACC | 1 cut(s) | 645 |
| PspGI | CCWGG | 1 cut(s) | 1642 |
| PspN4I | GGNNCC | 4 cut(s) | 268, 753, 1015, 1638 |
| PspPI | GGNCC | 2 cut(s) | 230, 267 |
| PspPPI | RGGWCCY | 1 cut(s) | 230 |
| PsrI | GAACNNNNNNTAC | 4 cut(s) | 7, 39, 90, 122 |
| PstI | CTGCAG | 2 cut(s) | 287, 859 |
| PstNI | CAGNNNCTG | 1 cut(s) | 347 |
| PsuI | RGATCY | 2 cut(s) | 343, 1194 |
| PsyI | GACNNNGTC | 1 cut(s) | 556 |
| PvuII | CAGCTG | 1 cut(s) | 1114 |
| RsaI | GTAC | 5 cut(s) | 575, 788, 1135, 1396, 1654 |
| RsaNI | GTAC | 5 cut(s) | 574, 787, 1134, 1395, 1653 |
| RseI | CAYNNNNRTG | 2 cut(s) | 43, 886 |
| SacI | GAGCTC | 2 cut(s) | 151, 186 |
| SapI | GCTCTTC | 2 cut(s) | 692, 1316 |
| SaqAI | TTAA | 4 cut(s) | 128, 174, 1514, 1668 |
| SatI | GCNGC | 3 cut(s) | 683, 1445, 1465 |
| Sau3AI | GATC | 8 cut(s) | 343, 583, 797, 831, 1036, 1188, 1194, 1600 |
| Sau96I | GGNCC | 2 cut(s) | 230, 267 |
| SchI | GAGTC | 3 cut(s) | 308, 1231, 1563 |
| ScrFI | CCNGG | 4 cut(s) | 1032, 1401, 1410, 1644 |
| SduI | GDGCHC | 3 cut(s) | 151, 186, 486 |
| SfaNI | GCATC | 4 cut(s) | 286, 501, 1459, 1508 |
| SfcI | CTRYAG | 2 cut(s) | 283, 855 |
| SfoI | GGCGCC | 1 cut(s) | 1015 |
| SinI | GGWCC | 1 cut(s) | 230 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 43, 886 |
| SmlI | CTYRAG | 2 cut(s) | 164, 1390 |
| SmoI | CTYRAG | 2 cut(s) | 164, 1390 |
| Sse9I | AATT | 6 cut(s) | 561, 638, 840, 1217, 1511, 1625 |
| SsiI | CCGC | 3 cut(s) | 1433, 1462, 1640 |
| SspDI | GGCGCC | 1 cut(s) | 1013 |
| SspMI | CTAG | 5 cut(s) | 29, 152, 467, 702, 1256 |
| SstI | GAGCTC | 2 cut(s) | 151, 186 |
| StyD4I | CCNGG | 4 cut(s) | 1030, 1399, 1408, 1642 |
| TaaI | ACNGT | 3 cut(s) | 220, 761, 1291 |
| TaiI | ACGT | 2 cut(s) | 103, 503 |
| TasI | AATT | 6 cut(s) | 561, 638, 840, 1217, 1511, 1625 |
| TatI | WGTACW | 2 cut(s) | 786, 1133 |
| TfiI | GAWTC | 3 cut(s) | 436, 985, 1648 |
| Tru1I | TTAA | 4 cut(s) | 128, 174, 1514, 1668 |
| Tru9I | TTAA | 4 cut(s) | 128, 174, 1514, 1668 |
| TscAI | CASTG | 4 cut(s) | 766, 864, 1323, 1579 |
| TseFI | GTSAC | 4 cut(s) | 645, 1234, 1258, 1574 |
| TseI | GCWGC | 3 cut(s) | 682, 1444, 1464 |
| Tsp45I | GTSAC | 4 cut(s) | 645, 1234, 1258, 1574 |
| TspDTI | ATGAA | 6 cut(s) | 82, 467, 1057, 1295, 1677, 1680 |
| TspRI | CASTG | 4 cut(s) | 766, 864, 1323, 1579 |
| Tth111I | GACNNNGTC | 1 cut(s) | 556 |
| VpaK11BI | GGWCC | 1 cut(s) | 230 |
| XapI | RAATTY | 1 cut(s) | 638 |
| XceI | RCATGY | 1 cut(s) | 1429 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 657 |
| XspI | CTAG | 5 cut(s) | 29, 152, 467, 702, 1256 |
| Zsp2I | ATGCAT | 2 cut(s) | 251, 1108 |