MD12G1200200.v1.1

Aminotransferase class-V

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0002899
Physical Location & Seq
Forward (+)
28104568 .. 28109173
4606 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1200200.v1.1.491

Sequence Viewer

Length: 1569 bp
ATGCTGTATGTAGTGAAAATTTTGCAGCTTGAGAAAGAGTCTTGGATGGAGGGTTTCCTCATGGACCACAATATGCTGTGGATCCGGAAGAGTTTACTCCTAGAGGTCATGGCGGAGAAGATTATCATGTTTTTCCGTTACTGTGTTCCTCAATTCCTCTGGCCCAACCTTTCCTTTCGAGTTTCGAGACCCTCTAATCGCCATTACCATAACCTTAATCATAATGACCCCCAAACCCAAGTCTCCGTTAAGCCGAAACCCTCTTCCTTCATCACAGACACCGAAATCCAATCCGAATTCTCCCACCACGCTGCCGGCGTTGCCAGGATCAACAATGGCAGCTTCGGTTGCTGCCCTGCCTCCGTCATCTCTTCCTTGCACCAATGGCAGCTCAAATTGCTCCGCCAGCCCGACGACTTCTACTTGAACGAGCTTCCAAATAGGATACTTGAATCGAGAACAGTAATCAAAGACCTCATCAGTGCAGAAGATGTGGAGGAAGTCTCCATTGTGGATAATGTCAGCACTGCTGTTGCTATGGTCCTGCAGCAGACGGCATGGTCTTTCGTGGAAGGGAAATTTGACAAAGGAGATGCTGTCATCATGTTTCGCTGCACTTATGGGGCGGTGAAGAACTCAATCAAGGCCTATTTTTTGCGCGCTGGTGGGTGTGCGATCGAAGTTCCATTTAATTTTCCGGTGACTTCAAACGAGGAAATCGTGAGTGAATTCAAGACAGCTTTGGGGAGAGAAAAGGGTATGGGGAGGAGAGTGAGACTGGCGGTGATAGATCACGTTACTTGCATGCCAAGCTTTGTAATGCCGGTTAAGGAATTGGTTAAAATTTGCAAGGAAGAAGGTGTTGAGCAAGTTTTAGTTGATGCAGCTCACGCTGTTGGCTGCGTAGATGTGGACATGAACGAAATCAGAGCCGATTTTTATACAGGTAATTTGTATAAGTGGTTTTTCTGCCCGCCCGCGGTTGCATTCGTGTACTGCAGGAAATCAGTTATGACACATTTAGATTTGCATCATCCTGTTGTGTCTCATGACTATGGAAACGGATTGGTCAAAGAAACCGGTTGGGTTGGGACGAGGGATTATAGTCCCTATCTCGTTCTTCCTTCGGTTATGGAGTTTGTCAACAGATTCGAAGGCGGTGCAGAGGGAATCAGGAACCGGAATCATGATGCTGTGGTTGAGATGGGGAAGATGCTTGCAGAAGCTTGGGGAACCAATCTAGGGTGTCCGCCGCAAATGTGTTCGAGCATGCTGATGGTTGGATTGCCGGCCAGTTTGGGGGTTCTGAGTGATGAAGATGCTATGAAGTTGTGGGAACTTCTGTGCAAGAAATTTGGTGTGGAAGTGAAAATACATTATCAAAACCCGAAAGATGGAGAGCTTGCACCTATAACTGGGTACGTTCGAATCTGCCACCAAATTTACAACAAATTTGAGGATTATTGCAGGCTCAGAGATGCAATCAACCAGCTCGTTCGCGATGACTTCAATTGCGGTAATCTTGAGATCATGAGGCCTGAAGAAGTCGGTCCGACGAATGACAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00270 map00270
Pfam Domains
Protein Families
Aminotran_5

Protein Analysis

523

Amino Acids

59.27

Weight (kDa)

5.92

Isoelectric Point (pI)

37.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_5 PF00266 149 - 341 7.2e-11 Aminotransferase class-V
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 559
AccII CGCG 3 cut(s) 660, 980, 1500
AccIII TCCGGA 1 cut(s) 84
AclWI GGATC 3 cut(s) 76, 89, 335
AcoI YGGCCR 1 cut(s) 1290
AcsI RAATTY 8 cut(s) 18, 296, 578, 728, 843, 1352, 1440, 1451
AcuI CTGAAG 1 cut(s) 1560
AfaI GTAC 2 cut(s) 995, 1421
AfiI CCNNNNNNNGG 5 cut(s) 979, 1242, 1299, 1394, 1415
AgeI ACCGGT 1 cut(s) 1079
AgsI TTSAA 5 cut(s) 427, 452, 708, 733, 1510
AjnI CCWGG 1 cut(s) 323
Alw26I GTCTC 5 cut(s) 181, 247, 508, 769, 1050
AlwI GGATC 3 cut(s) 76, 89, 335
Aor13HI TCCGGA 1 cut(s) 84
AoxI GGCC 4 cut(s) 161, 645, 1290, 1535
ApeKI GCWGC 9 cut(s) 25, 311, 339, 351, 388, 547, 612, 884, 900
ApoI RAATTY 8 cut(s) 18, 296, 578, 728, 843, 1352, 1440, 1451
AsiGI ACCGGT 1 cut(s) 1079
AspLEI GCGC 2 cut(s) 660, 662
AspS9I GGNCC 4 cut(s) 64, 162, 541, 1550
AsuHPI GGTGA 3 cut(s) 640, 712, 796
AsuII TTCGAA 2 cut(s) 1152, 1426
AvaII GGWCC 3 cut(s) 64, 541, 1550
BamHI GGATCC 1 cut(s) 81
BarI GAAGNNNNNNTAC 2 cut(s) 1356, 1388
BbvI GCAGC 9 cut(s) 37, 298, 338, 351, 400, 559, 599, 887, 896
BccI CCATC 4 cut(s) 40, 1198, 1270, 1388
BceAI ACGGC 1 cut(s) 570
BcgI CGANNNNNNTGC 2 cut(s) 1142, 1176
BciT130I CCWGG 1 cut(s) 325
BciVI GTATCC 1 cut(s) 438
BcoDI GTCTC 5 cut(s) 181, 247, 508, 769, 1050
BfaI CTAG 2 cut(s) 101, 1241
BfmI CTRYAG 2 cut(s) 545, 997
BfuI GTATCC 1 cut(s) 438
Bme1390I CCNGG 1 cut(s) 325
Bme18I GGWCC 3 cut(s) 64, 541, 1550
BmgT120I GGNCC 4 cut(s) 64, 162, 541, 1550
BmiI GGNNCC 3 cut(s) 83, 1178, 1234
BmrFI CCNGG 1 cut(s) 325
BmrI ACTGGG 1 cut(s) 1425
BmsI GCATC 7 cut(s) 583, 871, 1039, 1180, 1203, 1309, 1468
BmuI ACTGGG 1 cut(s) 1425
BplI GAGNNNNNCTC 2 cut(s) 488, 520
Bpu14I TTCGAA 2 cut(s) 1152, 1426
BpuEI CTTGAG 2 cut(s) 50, 1544
BsaBI GATNNNNATC 1 cut(s) 1029
BsaI GGTCTC 1 cut(s) 181
BsaJI CCNNGG 1 cut(s) 978
BsaWI WCCGGW 4 cut(s) 84, 697, 1079, 1179
BsaXI ACNNNNNCTCC 2 cut(s) 582, 612
Bsc4I CCNNNNNNNGG 5 cut(s) 979, 1242, 1299, 1394, 1415
Bse118I RCCGGY 4 cut(s) 314, 823, 1079, 1288
Bse1I ACTGG 3 cut(s) 783, 1293, 1420
Bse8I GATNNNNATC 1 cut(s) 1029
BseAI TCCGGA 1 cut(s) 84
BseBI CCWGG 1 cut(s) 325
BseDI CCNNGG 1 cut(s) 978
BseGI GGATG 2 cut(s) 51, 1033
BseJI GATNNNNATC 1 cut(s) 1029
BseLI CCNNNNNNNGG 5 cut(s) 979, 1242, 1299, 1394, 1415
BseMII CTCAG 2 cut(s) 1298, 1486
BseNI ACTGG 3 cut(s) 783, 1293, 1420
BsePI GCGCGC 1 cut(s) 658
BseRI GAGGAG 1 cut(s) 781
BseXI GCAGC 9 cut(s) 37, 298, 338, 351, 400, 559, 599, 887, 896
BsgI GTGCAG 3 cut(s) 504, 598, 1182
Bsh1236I CGCG 3 cut(s) 660, 980, 1500
Bsh1285I CGRYCG 1 cut(s) 678
BshFI GGCC 4 cut(s) 163, 647, 1292, 1537
BshTI ACCGGT 1 cut(s) 1079
BsiEI CGRYCG 1 cut(s) 678
BsiSI CCGG 7 cut(s) 85, 315, 698, 824, 1080, 1180, 1289
BslFI GGGAC 2 cut(s) 1092, 1105
BslI CCNNNNNNNGG 5 cut(s) 979, 1242, 1299, 1394, 1415
BsmAI GTCTC 5 cut(s) 181, 247, 508, 769, 1050
BsmFI GGGAC 2 cut(s) 1092, 1105
BsmI GAATGC 1 cut(s) 986
BsnI GGCC 4 cut(s) 163, 647, 1292, 1537
Bso31I GGTCTC 1 cut(s) 181
Bsp119I TTCGAA 2 cut(s) 1152, 1426
Bsp13I TCCGGA 1 cut(s) 84
Bsp143I GATC 5 cut(s) 81, 327, 675, 790, 1527
Bsp68I TCGCGA 1 cut(s) 1500
BspANI GGCC 4 cut(s) 163, 647, 1292, 1537
BspCNI CTCAG 2 cut(s) 1299, 1485
BspEI TCCGGA 1 cut(s) 84
BspFNI CGCG 3 cut(s) 660, 980, 1500
BspHI TCATGA 3 cut(s) 1048, 1186, 1530
BspLI GGNNCC 3 cut(s) 83, 1178, 1234
BspMAI CTGCAG 2 cut(s) 549, 1001
BspPI GGATC 3 cut(s) 76, 89, 335
BspT104I TTCGAA 2 cut(s) 1152, 1426
BspTNI GGTCTC 1 cut(s) 181
BsrFI RCCGGY 4 cut(s) 314, 823, 1079, 1288
BsrI ACTGG 3 cut(s) 783, 1293, 1420
BssAI RCCGGY 4 cut(s) 314, 823, 1079, 1288
BssECI CCNNGG 1 cut(s) 978
BssHII GCGCGC 1 cut(s) 658
BssMI GATC 5 cut(s) 81, 327, 675, 790, 1527
Bst2UI CCWGG 1 cut(s) 325
Bst4CI ACNGT 2 cut(s) 143, 463
Bst6I CTCTTC 3 cut(s) 83, 268, 376
BstBI TTCGAA 2 cut(s) 1152, 1426
BstDEI CTNAG 2 cut(s) 1307, 1472
BstDSI CCRYGG 1 cut(s) 978
BstF5I GGATG 2 cut(s) 51, 1033
BstFNI CGCG 3 cut(s) 660, 980, 1500
BstHHI GCGC 2 cut(s) 660, 662
BstKTI GATC 5 cut(s) 84, 330, 678, 793, 1530
BstMAI GTCTC 5 cut(s) 181, 247, 508, 769, 1050
BstMBI GATC 5 cut(s) 81, 327, 675, 790, 1527
BstMCI CGRYCG 1 cut(s) 678
BstMWI GCNNNNNNNGC 7 cut(s) 320, 348, 385, 397, 406, 810, 890
BstNI CCWGG 1 cut(s) 325
BstNSI RCATGY 2 cut(s) 808, 1273
BstSCI CCNGG 1 cut(s) 323
BstSFI CTRYAG 2 cut(s) 545, 997
BstUI CGCG 3 cut(s) 660, 980, 1500
BstV1I GCAGC 9 cut(s) 37, 298, 338, 351, 400, 559, 599, 887, 896
BstX2I RGATCY 1 cut(s) 81
BstYI RGATCY 1 cut(s) 81
BsuI GTATCC 1 cut(s) 438
BsuRI GGCC 4 cut(s) 163, 647, 1292, 1537
BtgI CCRYGG 1 cut(s) 978
BtgZI GCGATG 1 cut(s) 1515
BtsCI GGATG 2 cut(s) 51, 1033
BtsI GCAGTG 1 cut(s) 525
BtsIMutI CAGTG 2 cut(s) 487, 525
BtuMI TCGCGA 1 cut(s) 1500
CciI TCATGA 3 cut(s) 1048, 1186, 1530
CfoI GCGC 2 cut(s) 660, 662
Cfr10I RCCGGY 4 cut(s) 314, 823, 1079, 1288
Cfr13I GGNCC 4 cut(s) 64, 162, 541, 1550
Cfr42I CCGCGG 1 cut(s) 981
CpoI CGGWCCG 1 cut(s) 1550
Csp6I GTAC 2 cut(s) 994, 1420
CspAI ACCGGT 1 cut(s) 1079
CspI CGGWCCG 1 cut(s) 1550
CviQI GTAC 2 cut(s) 994, 1420
DdeI CTNAG 2 cut(s) 1307, 1472
DpnI GATC 5 cut(s) 83, 329, 677, 792, 1529
DpnII GATC 5 cut(s) 81, 327, 675, 790, 1527
DrdI GACNNNNNNGTC 1 cut(s) 559
DseDI GACNNNNNNGTC 1 cut(s) 559
EaeI YGGCCR 1 cut(s) 1290
Eam1104I CTCTTC 3 cut(s) 83, 268, 376
EarI CTCTTC 3 cut(s) 83, 268, 376
EciI GGCGGA 3 cut(s) 128, 392, 1239
Eco147I AGGCCT 2 cut(s) 647, 1537
Eco31I GGTCTC 1 cut(s) 181
Eco47I GGWCC 3 cut(s) 64, 541, 1550
Eco57I CTGAAG 1 cut(s) 1560
EcoRI GAATTC 2 cut(s) 296, 728
EcoRII CCWGG 1 cut(s) 323
FaqI GGGAC 2 cut(s) 1092, 1105
FauI CCCGC 2 cut(s) 981, 985
FokI GGATG 2 cut(s) 58, 1020
FspBI CTAG 2 cut(s) 101, 1241
GlaI GCGC 2 cut(s) 659, 661
HaeIII GGCC 4 cut(s) 163, 647, 1292, 1537
HapII CCGG 7 cut(s) 85, 315, 698, 824, 1080, 1180, 1289
HhaI GCGC 2 cut(s) 660, 662
Hin6I GCGC 2 cut(s) 658, 660
HinP1I GCGC 2 cut(s) 658, 660
HincII GTYRAC 1 cut(s) 1144
HindII GTYRAC 1 cut(s) 1144
HindIII AAGCTT 2 cut(s) 811, 1224
HinfI GANTC 6 cut(s) 38, 452, 1149, 1170, 1183, 1428
HpaII CCGG 7 cut(s) 85, 315, 698, 824, 1080, 1180, 1289
HphI GGTGA 3 cut(s) 640, 712, 796
Hpy166II GTNNAC 4 cut(s) 95, 913, 994, 1144
Hpy188I TCNGA 5 cut(s) 295, 929, 1308, 1475, 1554
Hpy8I GTNNAC 4 cut(s) 95, 913, 994, 1144
Hpy99I CGWCG 2 cut(s) 416, 1558
HpyAV CCTTC 5 cut(s) 277, 566, 851, 1134, 1148
HpyCH4III ACNGT 2 cut(s) 143, 463
HpyCH4IV ACGT 2 cut(s) 795, 1422
HpyF10VI GCNNNNNNNGC 7 cut(s) 320, 348, 385, 397, 406, 810, 890
HpyF3I CTNAG 2 cut(s) 1307, 1472
HpySE526I ACGT 2 cut(s) 795, 1422
HspAI GCGC 2 cut(s) 658, 660
Kpn2I TCCGGA 1 cut(s) 84
KroI GCCGGC 2 cut(s) 314, 1288
KroNI GCCGGC 2 cut(s) 316, 1290
KspI CCGCGG 1 cut(s) 981
Kzo9I GATC 5 cut(s) 81, 327, 675, 790, 1527
LmnI GCTCC 1 cut(s) 405
Lsp1109I GCAGC 9 cut(s) 37, 298, 338, 351, 400, 559, 599, 887, 896
LweI GCATC 7 cut(s) 583, 871, 1039, 1180, 1203, 1309, 1468
MaeI CTAG 2 cut(s) 101, 1241
MaeII ACGT 2 cut(s) 795, 1422
MaeIII GTNAC 3 cut(s) 137, 700, 796
MalI GATC 5 cut(s) 83, 329, 677, 792, 1529
MboI GATC 5 cut(s) 81, 327, 675, 790, 1527
MfeI CAATTG 1 cut(s) 1510
MflI RGATCY 1 cut(s) 81
MlyI GAGTC 1 cut(s) 47
MmeI TCCRAC 1 cut(s) 1261
MroI TCCGGA 1 cut(s) 84
MroNI GCCGGC 2 cut(s) 314, 1288
MseI TTAA 5 cut(s) 216, 249, 690, 828, 840
MslI CAYNNNNRTG 2 cut(s) 1053, 1274
MspA1I CMGCKG 1 cut(s) 980
MspI CCGG 7 cut(s) 85, 315, 698, 824, 1080, 1180, 1289
MspR9I CCNGG 1 cut(s) 325
MunI CAATTG 1 cut(s) 1510
Mva1269I GAATGC 1 cut(s) 986
MvaI CCWGG 1 cut(s) 325
MvnI CGCG 3 cut(s) 660, 980, 1500
MwoI GCNNNNNNNGC 7 cut(s) 320, 348, 385, 397, 406, 810, 890
NaeI GCCGGC 2 cut(s) 316, 1290
NdeII GATC 5 cut(s) 81, 327, 675, 790, 1527
NgoMIV GCCGGC 2 cut(s) 314, 1288
NlaIV GGNNCC 3 cut(s) 83, 1178, 1234
NmuCI GTSAC 1 cut(s) 700
NruI TCGCGA 1 cut(s) 1500
NspI RCATGY 2 cut(s) 808, 1273
NspV TTCGAA 2 cut(s) 1152, 1426
PaeI GCATGC 2 cut(s) 808, 1273
PagI TCATGA 3 cut(s) 1048, 1186, 1530
PauI GCGCGC 1 cut(s) 658
PceI AGGCCT 2 cut(s) 647, 1537
PcsI WCGNNNNNNNCGW 2 cut(s) 315, 717
PctI GAATGC 1 cut(s) 986
PdiI GCCGGC 2 cut(s) 316, 1290
PfeI GAWTC 5 cut(s) 452, 1149, 1170, 1183, 1428
PinAI ACCGGT 1 cut(s) 1079
Ple19I CGATCG 1 cut(s) 678
PleI GAGTC 1 cut(s) 46
PpsI GAGTC 1 cut(s) 46
Psp6I CCWGG 1 cut(s) 323
PspGI CCWGG 1 cut(s) 323
PspN4I GGNNCC 3 cut(s) 83, 1178, 1234
PspPI GGNCC 4 cut(s) 64, 162, 541, 1550
PstI CTGCAG 2 cut(s) 549, 1001
PsuI RGATCY 1 cut(s) 81
PteI GCGCGC 1 cut(s) 658
PvuI CGATCG 1 cut(s) 678
RruI TCGCGA 1 cut(s) 1500
RsaI GTAC 2 cut(s) 995, 1421
RsaNI GTAC 2 cut(s) 994, 1420
RseI CAYNNNNRTG 2 cut(s) 1053, 1274
Rsr2I CGGWCCG 1 cut(s) 1550
RsrII CGGWCCG 1 cut(s) 1550
SacII CCGCGG 1 cut(s) 981
SaqAI TTAA 5 cut(s) 216, 249, 690, 828, 840
Sau3AI GATC 5 cut(s) 81, 327, 675, 790, 1527
Sau96I GGNCC 4 cut(s) 64, 162, 541, 1550
SchI GAGTC 1 cut(s) 47
ScrFI CCNGG 1 cut(s) 325
SfaNI GCATC 7 cut(s) 583, 871, 1039, 1180, 1203, 1309, 1468
SfcI CTRYAG 2 cut(s) 545, 997
Sfr303I CCGCGG 1 cut(s) 981
SfuI TTCGAA 2 cut(s) 1152, 1426
SgrBI CCGCGG 1 cut(s) 981
SinI GGWCC 3 cut(s) 64, 541, 1550
SmiMI CAYNNNNRTG 2 cut(s) 1053, 1274
SmlI CTYRAG 2 cut(s) 29, 1523
SmoI CTYRAG 2 cut(s) 29, 1523
SphI GCATGC 2 cut(s) 808, 1273
SseBI AGGCCT 2 cut(s) 647, 1537
SspMI CTAG 2 cut(s) 101, 1241
StuI AGGCCT 2 cut(s) 647, 1537
StyD4I CCNGG 1 cut(s) 323
TaaI ACNGT 2 cut(s) 143, 463
TaiI ACGT 2 cut(s) 798, 1425
TaqI TCGA 7 cut(s) 178, 185, 455, 678, 1152, 1265, 1426
TaqII GACCGA 1 cut(s) 1538
TatI WGTACW 1 cut(s) 993
TauI GCSGC 1 cut(s) 1255
TfiI GAWTC 5 cut(s) 452, 1149, 1170, 1183, 1428
Tru1I TTAA 5 cut(s) 216, 249, 690, 828, 840
Tru9I TTAA 5 cut(s) 216, 249, 690, 828, 840
TscAI CASTG 2 cut(s) 487, 532
TseFI GTSAC 1 cut(s) 700
TseI GCWGC 9 cut(s) 25, 311, 339, 351, 388, 547, 612, 884, 900
Tsp45I GTSAC 1 cut(s) 700
TspDTI ATGAA 4 cut(s) 259, 932, 1329, 1340
TspGWI ACGGA 4 cut(s) 125, 235, 352, 1077
TspRI CASTG 2 cut(s) 487, 532
VpaK11BI GGWCC 3 cut(s) 64, 541, 1550
XapI RAATTY 8 cut(s) 18, 296, 578, 728, 843, 1352, 1440, 1451
XceI RCATGY 2 cut(s) 808, 1273
XspI CTAG 2 cut(s) 101, 1241
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.