Rorug03G0028700

Aminotransferase class-V

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0002899
Physical Location & Seq
Reverse (-)
2295433 .. 2295946
514 bp
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UTR
Exon/CDS
Intron
Rorug03G0028700.1

Sequence Viewer

Length: 411 bp
ATGGCCGGGAAGATGAGGAATATAGTTTTGTTACTTATTGTCTGTGCAACAATGGGAAGTGCTTCTGCCCAAAGTGCTTCTGATGTGAGATCCACATTCCACCACTACAATCCGCAGGATAACAACTGGGACTTGCTTGCAGTAGATGCCTTCTGCGCCACATTTGATGAGGATCGGCCCTTCGAATGGCGCAGCAGATATGGATGGACAACCTTCTGCGGACCTAATGGCCCTAGTGGAGCAGACGCTTGCGGAAAATGCCTACCGGAAACAGTAAGAATCATCGATCAGTGCAGCAATGGAGGTTTGGATTTGGATGTGAATGTGTTTAACCAGATAGACACAGATGGAAGAGGCTATGCGGAAGGACACCTTGTTGTCAACTATGACTTTGTGGACTGCGGCGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00270 map00270
Pfam Domains
Protein Families
Aminotran_5

Protein Analysis

136

Amino Acids

14.91

Weight (kDa)

4.42

Isoelectric Point (pI)

38.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Barwin PF00967 25 - 134 3.3e-44 Barwin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 113, 219, 252, 362, 402
AclWI GGATC 2 cut(s) 84, 180
AcoI YGGCCR 1 cut(s) 3
AfiI CCNNNNNNNGG 1 cut(s) 186
AlwI GGATC 2 cut(s) 84, 180
AoxI GGCC 3 cut(s) 3, 176, 229
ApeKI GCWGC 2 cut(s) 192, 294
Asp700I GAANNNNTTC 1 cut(s) 61
AspLEI GCGC 2 cut(s) 158, 192
AspS9I GGNCC 3 cut(s) 177, 221, 230
AsuC2I CCSGG 1 cut(s) 7
AsuII TTCGAA 1 cut(s) 183
AvaII GGWCC 1 cut(s) 221
BbvI GCAGC 2 cut(s) 204, 306
BccI CCATC 2 cut(s) 198, 341
BcnI CCSGG 1 cut(s) 7
BfaI CTAG 1 cut(s) 234
BisI GCNGC 3 cut(s) 193, 295, 403
BlsI GCNGC 3 cut(s) 194, 296, 404
Bme1390I CCNGG 1 cut(s) 7
Bme18I GGWCC 1 cut(s) 221
BmgT120I GGNCC 3 cut(s) 177, 221, 230
BmrFI CCNGG 1 cut(s) 7
BmrI ACTGGG 1 cut(s) 136
BmsI GCATC 1 cut(s) 136
BmuI ACTGGG 1 cut(s) 136
Bpu14I TTCGAA 1 cut(s) 183
BpuMI CCSGG 1 cut(s) 7
Bsa29I ATCGAT 1 cut(s) 285
BsaBI GATNNNNATC 1 cut(s) 171
BsaWI WCCGGW 1 cut(s) 265
Bsc4I CCNNNNNNNGG 1 cut(s) 186
Bse1I ACTGG 1 cut(s) 131
Bse3DI GCAATG 1 cut(s) 304
Bse8I GATNNNNATC 1 cut(s) 171
BseCI ATCGAT 1 cut(s) 285
BseGI GGATG 2 cut(s) 209, 322
BseJI GATNNNNATC 1 cut(s) 171
BseLI CCNNNNNNNGG 1 cut(s) 186
BseMI GCAATG 1 cut(s) 304
BseNI ACTGG 1 cut(s) 131
BseXI GCAGC 2 cut(s) 204, 306
BsgI GTGCAG 1 cut(s) 313
BshFI GGCC 3 cut(s) 5, 178, 231
BshVI ATCGAT 1 cut(s) 285
BsiSI CCGG 2 cut(s) 6, 266
BslFI GGGAC 1 cut(s) 143
BslI CCNNNNNNNGG 1 cut(s) 186
BsmFI GGGAC 1 cut(s) 143
BsnI GGCC 3 cut(s) 5, 178, 231
Bsp119I TTCGAA 1 cut(s) 183
Bsp143I GATC 3 cut(s) 89, 172, 286
BspACI CCGC 5 cut(s) 113, 219, 252, 362, 402
BspANI GGCC 3 cut(s) 5, 178, 231
BspDI ATCGAT 1 cut(s) 285
BspPI GGATC 2 cut(s) 84, 180
BspT104I TTCGAA 1 cut(s) 183
BsrDI GCAATG 1 cut(s) 304
BsrI ACTGG 1 cut(s) 131
BssMI GATC 3 cut(s) 89, 172, 286
Bst4CI ACNGT 1 cut(s) 274
Bst6I CTCTTC 1 cut(s) 346
BstAPI GCANNNNNTGC 1 cut(s) 146
BstBI TTCGAA 1 cut(s) 183
BstC8I GCNNGC 2 cut(s) 138, 250
BstF5I GGATG 2 cut(s) 209, 322
BstHHI GCGC 2 cut(s) 158, 192
BstKTI GATC 3 cut(s) 92, 175, 289
BstMBI GATC 3 cut(s) 89, 172, 286
BstMWI GCNNNNNNNGC 4 cut(s) 74, 146, 155, 258
BstSCI CCNGG 1 cut(s) 5
BstV1I GCAGC 2 cut(s) 204, 306
BstX2I RGATCY 1 cut(s) 89
BstYI RGATCY 1 cut(s) 89
Bsu15I ATCGAT 1 cut(s) 285
BsuRI GGCC 3 cut(s) 5, 178, 231
BsuTUI ATCGAT 1 cut(s) 285
BtsCI GGATG 2 cut(s) 209, 322
BtsIMutI CAGTG 1 cut(s) 296
Cac8I GCNNGC 2 cut(s) 138, 250
CfoI GCGC 2 cut(s) 158, 192
Cfr13I GGNCC 3 cut(s) 177, 221, 230
ClaI ATCGAT 1 cut(s) 285
CseI GACGC 1 cut(s) 254
CviJI RGCY 4 cut(s) 5, 178, 231, 357
CviKI_1 RGCY 4 cut(s) 5, 178, 231, 357
DpnI GATC 3 cut(s) 91, 174, 288
DpnII GATC 3 cut(s) 89, 172, 286
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 346
EarI CTCTTC 1 cut(s) 346
Eco47I GGWCC 1 cut(s) 221
FaiI YATR 4 cut(s) 23, 201, 360, 387
FalI AAGNNNNNCTT 2 cut(s) 357, 389
FaqI GGGAC 1 cut(s) 143
Fnu4HI GCNGC 3 cut(s) 193, 295, 403
FokI GGATG 2 cut(s) 216, 329
Fsp4HI GCNGC 3 cut(s) 193, 295, 403
FspBI CTAG 1 cut(s) 234
GlaI GCGC 2 cut(s) 157, 191
GluI GCNGC 3 cut(s) 193, 295, 403
HaeIII GGCC 3 cut(s) 5, 178, 231
HapII CCGG 2 cut(s) 6, 266
HgaI GACGC 1 cut(s) 254
HhaI GCGC 2 cut(s) 158, 192
Hin6I GCGC 2 cut(s) 156, 190
HinP1I GCGC 2 cut(s) 156, 190
HincII GTYRAC 1 cut(s) 382
HindII GTYRAC 1 cut(s) 382
HinfI GANTC 1 cut(s) 279
HpaII CCGG 2 cut(s) 6, 266
Hpy166II GTNNAC 2 cut(s) 382, 397
Hpy188I TCNGA 1 cut(s) 82
Hpy8I GTNNAC 2 cut(s) 382, 397
HpyAV CCTTC 4 cut(s) 160, 190, 223, 359
HpyCH4III ACNGT 1 cut(s) 274
HpyCH4V TGCA 3 cut(s) 47, 140, 294
HpyF10VI GCNNNNNNNGC 4 cut(s) 74, 146, 155, 258
HspAI GCGC 2 cut(s) 156, 190
Kzo9I GATC 3 cut(s) 89, 172, 286
LmnI GCTCC 1 cut(s) 239
LpnPI CCDG 5 cut(s) 19, 101, 112, 279, 347
Lsp1109I GCAGC 2 cut(s) 204, 306
LweI GCATC 1 cut(s) 136
MaeI CTAG 1 cut(s) 234
MaeIII GTNAC 1 cut(s) 30
MalI GATC 3 cut(s) 91, 174, 288
MboI GATC 3 cut(s) 89, 172, 286
MboII GAAGA 2 cut(s) 22, 363
MflI RGATCY 1 cut(s) 89
MnlI CCTC 4 cut(s) 9, 163, 296, 347
MroXI GAANNNNTTC 1 cut(s) 61
MseI TTAA 1 cut(s) 330
MspI CCGG 2 cut(s) 6, 266
MspR9I CCNGG 1 cut(s) 7
MwoI GCNNNNNNNGC 4 cut(s) 74, 146, 155, 258
NciI CCSGG 1 cut(s) 7
NdeII GATC 3 cut(s) 89, 172, 286
NspV TTCGAA 1 cut(s) 183
PdmI GAANNNNTTC 1 cut(s) 61
PfeI GAWTC 1 cut(s) 279
PkrI GCNGC 3 cut(s) 194, 296, 404
PspPI GGNCC 3 cut(s) 177, 221, 230
PsuI RGATCY 1 cut(s) 89
SaqAI TTAA 1 cut(s) 330
SatI GCNGC 3 cut(s) 193, 295, 403
Sau3AI GATC 3 cut(s) 89, 172, 286
Sau96I GGNCC 3 cut(s) 177, 221, 230
ScrFI CCNGG 1 cut(s) 7
SetI ASST 4 cut(s) 215, 226, 307, 375
SfaNI GCATC 1 cut(s) 136
SfuI TTCGAA 1 cut(s) 183
SinI GGWCC 1 cut(s) 221
SsiI CCGC 5 cut(s) 113, 219, 252, 362, 402
SspMI CTAG 1 cut(s) 234
StyD4I CCNGG 1 cut(s) 5
TaaI ACNGT 1 cut(s) 274
TaqI TCGA 2 cut(s) 183, 285
TauI GCSGC 1 cut(s) 405
TfiI GAWTC 1 cut(s) 279
Tru1I TTAA 1 cut(s) 330
Tru9I TTAA 1 cut(s) 330
TscAI CASTG 1 cut(s) 296
TseI GCWGC 2 cut(s) 192, 294
TspRI CASTG 1 cut(s) 296
VpaK11BI GGWCC 1 cut(s) 221
XmnI GAANNNNTTC 1 cut(s) 61
XspI CTAG 1 cut(s) 234
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.