Rw3G007450

Aminotransferase class-V

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0002899
Physical Location & Seq
Reverse (-)
6872635 .. 6875167
2533 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G007450.1

Sequence Viewer

Length: 1533 bp
ATGGCTTGCCGGATAATCATGTTCTACCGGTACTATGTTGCTCATCTACGACTTTGTCATCAGAATATCCAAATCTTGAAGCCCAAGCTTTCATTTCGAATTTCAAGGGCATCCTCTTACCATTATTACCACAACCTTAACCCTACTGATACCCAAACCAATTTCCCCATCAAGCCAAAACTTTCTTCCTTCATTACAGATTCTGAAATCGAAGCTGAATTTTCTCACCATGATCCGGGTGTTGCCCGGATAAACAATGGCAGCTTTGGTTGCTCCCCTGCTTTTGTTATCTCTGAATTGCAACAATGGCAACTCAAATGGCTGCGCCAGCCGGACCATTTCTTATTCAATGAGCTCCACAAGGGGATACTCAAATCAAGAAACACAGTTAAAGAACTTATCAATGCAGAGGATGTTGATGAAATTTCCATTGTAGATAATGCCACCACAGCAGTAGCCATAGTCTTGCAGCAGATGCAGTGGGCCATCTTTGAAGGCAAATTCAAGAAAGGGGATGCTGTCATCATACTGCAGTGTGCTTATGGCGCGGTGAAGAACTCCATCAAGGCCTATTTTTTGCGTGCTGGCGGGTATGTTATTGAAGTTCCATTTCAATTTCCAGTGAATTCCAGTGAAGAAATTATAAGTGAATTTCGGATGGCTTTGGTGAAGGAAAAGGCTAGTGGTAGAAGAGTTAGATTAGCTATGATTGATCATGTTACATCCATGCCATCTGTTGTACTTCCAGTTAAGAAATTGGTTAAAGTTTGCAGGGAAGAAGGTGTTGATCAAGTTTTCATAGATGGGGCTCATGGTGTTGGGTGTGTAGATGTTGACATGCAAGAAATTGGAGCAGATTTTTACACTAGTAATTTGCACAAGTGGTTCTTCTGCCCTGCTTCGGTTGCATTTTTATATTGTAGGAAGTCAGTCACACATTTAGAATTGCATCATCCTATGGTGTCTCATGACTATGGTAAAGGGTTGGCTATTGAAAGTAGTTGGATAGGGACTAGGGATTATAGTCCATCTTTAGTGGTTCCTTCAGTTGTGGAGTTCACAAATAGGTTTGAAGGCGGTATTGAGGGAATCAAAAGGAGGAATCATGATGCTGTTGTTGAGATGGGTAAGATGTTGGCAAAGGCTTGGGGAACCAATCTTGGGTCTCCACCAGATATGTGTGCGAGCATGATCATGGTCGGACTACCGACTTGTTTGGGGATTTCAAGTAATGATGATGCTTTGAAGTTAAGGACACATCTACGGGAGCAATTTGGTGTTGAAGTCCCAATATATTACCAGATGCCGAAACATGAAGAGGTTGCAACAACAACAGGCTATGCTCGAATTTCTTATCAAGTCTACAACAAAATTGATGACTATTATAAGTTCAGAGATGCAATTAATCAACTTGTTTGTGATGGTTTCACCTGTGCTCAATCTTTAGACATATATCTTGCAATGGTGATAATATTGCCCCAAAAATCCATTTTTCCACTTTCATCTTGTGTCAATGATGATCAACAAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00270 map00270
Pfam Domains
Protein Families
Aminotran_5

Protein Analysis

510

Amino Acids

57.71

Weight (kDa)

6.66

Isoelectric Point (pI)

43.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_5 PF00266 108 - 312 1e-19 Aminotransferase class-V
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 644, 1386
AccI GTMKAC 1 cut(s) 1362
AccII CGCG 1 cut(s) 548
AciI CCGC 3 cut(s) 548, 588, 1077
AclWI GGATC 1 cut(s) 227
AcsI RAATTY 7 cut(s) 99, 218, 423, 500, 625, 650, 1347
AcuI CTGAAG 1 cut(s) 1029
AfaI GTAC 2 cut(s) 32, 741
AfiI CCNNNNNNNGG 3 cut(s) 235, 901, 1161
AgeI ACCGGT 1 cut(s) 27
AhlI ACTAGT 1 cut(s) 866
AjuI GAANNNNNNNTTGG 2 cut(s) 169, 201
AluBI AGCT 5 cut(s) 88, 215, 264, 355, 704
AluI AGCT 5 cut(s) 88, 215, 264, 355, 704
Alw21I GWGCWC 2 cut(s) 357, 1438
Alw26I GTCTC 2 cut(s) 969, 1170
AlwI GGATC 1 cut(s) 227
AlwNI CAGNNNCTG 1 cut(s) 203
AoxI GGCC 2 cut(s) 483, 567
ApeKI GCWGC 3 cut(s) 261, 322, 469
ApoI RAATTY 7 cut(s) 99, 218, 423, 500, 625, 650, 1347
AseI ATTAAT 1 cut(s) 1404
AsiGI ACCGGT 1 cut(s) 27
AspLEI GCGC 2 cut(s) 327, 548
AspS9I GGNCC 2 cut(s) 334, 483
AsuC2I CCSGG 2 cut(s) 237, 247
AsuHPI GGTGA 5 cut(s) 218, 562, 679, 1420, 1477
AsuII TTCGAA 1 cut(s) 97
AvaII GGWCC 1 cut(s) 334
BanII GRGCYC 2 cut(s) 357, 811
Bbv12I GWGCWC 2 cut(s) 357, 1438
BbvI GCAGC 3 cut(s) 273, 309, 481
BccI CCATC 9 cut(s) 176, 494, 569, 652, 739, 797, 1036, 1117, 1415
BciVI GTATCC 1 cut(s) 360
BclI TGATCA 4 cut(s) 712, 787, 1191, 1519
BcnI CCSGG 2 cut(s) 237, 247
BcoDI GTCTC 2 cut(s) 969, 1170
BcuI ACTAGT 1 cut(s) 866
BfaI CTAG 3 cut(s) 681, 867, 1014
BfmI CTRYAG 1 cut(s) 530
BfuI GTATCC 1 cut(s) 360
BisI GCNGC 3 cut(s) 262, 323, 470
BlsI GCNGC 3 cut(s) 263, 324, 471
Bme1390I CCNGG 2 cut(s) 237, 247
Bme18I GGWCC 1 cut(s) 334
BmgT120I GGNCC 2 cut(s) 334, 483
BmiI GGNNCC 2 cut(s) 1041, 1153
BmrFI CCNGG 2 cut(s) 237, 247
BmsI GCATC 8 cut(s) 119, 465, 505, 958, 1099, 1228, 1293, 1387
Bpu14I TTCGAA 1 cut(s) 97
BpuMI CCSGG 2 cut(s) 237, 247
BsaI GGTCTC 1 cut(s) 1170
BsaWI WCCGGW 1 cut(s) 27
Bsc4I CCNNNNNNNGG 3 cut(s) 235, 901, 1161
Bse118I RCCGGY 1 cut(s) 27
Bse1I ACTGG 3 cut(s) 620, 630, 746
Bse3DI GCAATG 1 cut(s) 1467
BseGI GGATG 6 cut(s) 110, 418, 520, 663, 722, 952
BseLI CCNNNNNNNGG 3 cut(s) 235, 901, 1161
BseMI GCAATG 1 cut(s) 1467
BseNI ACTGG 3 cut(s) 620, 630, 746
BseXI GCAGC 3 cut(s) 273, 309, 481
Bsh1236I CGCG 1 cut(s) 548
BshFI GGCC 2 cut(s) 485, 569
BshTI ACCGGT 1 cut(s) 27
BsiHKAI GWGCWC 2 cut(s) 357, 1438
BsiSI CCGG 5 cut(s) 10, 28, 236, 247, 332
BslFI GGGAC 2 cut(s) 1024, 1271
BslI CCNNNNNNNGG 3 cut(s) 235, 901, 1161
BsmAI GTCTC 2 cut(s) 969, 1170
BsmFI GGGAC 2 cut(s) 1024, 1271
BsnI GGCC 2 cut(s) 485, 569
Bso31I GGTCTC 1 cut(s) 1170
Bsp119I TTCGAA 1 cut(s) 97
Bsp1286I GDGCHC 3 cut(s) 357, 811, 1438
Bsp143I GATC 5 cut(s) 232, 712, 787, 1191, 1519
BspACI CCGC 3 cut(s) 548, 588, 1077
BspANI GGCC 2 cut(s) 485, 569
BspFNI CGCG 1 cut(s) 548
BspHI TCATGA 2 cut(s) 967, 1105
BspLI GGNNCC 2 cut(s) 1041, 1153
BspMAI CTGCAG 1 cut(s) 534
BspPI GGATC 1 cut(s) 227
BspT104I TTCGAA 1 cut(s) 97
BspTNI GGTCTC 1 cut(s) 1170
BsrDI GCAATG 1 cut(s) 1467
BsrFI RCCGGY 1 cut(s) 27
BsrI ACTGG 3 cut(s) 620, 630, 746
BssAI RCCGGY 1 cut(s) 27
BssMI GATC 5 cut(s) 232, 712, 787, 1191, 1519
Bst4CI ACNGT 1 cut(s) 388
Bst6I CTCTTC 2 cut(s) 685, 1311
BstAPI GCANNNNNTGC 1 cut(s) 475
BstBI TTCGAA 1 cut(s) 97
BstC8I GCNNGC 5 cut(s) 7, 329, 582, 586, 1186
BstF5I GGATG 6 cut(s) 110, 418, 520, 663, 722, 952
BstFNI CGCG 1 cut(s) 548
BstHHI GCGC 2 cut(s) 327, 548
BstKTI GATC 5 cut(s) 235, 715, 790, 1194, 1522
BstMAI GTCTC 2 cut(s) 969, 1170
BstMBI GATC 5 cut(s) 232, 712, 787, 1191, 1519
BstMWI GCNNNNNNNGC 7 cut(s) 270, 307, 328, 449, 475, 545, 905
BstNSI RCATGY 1 cut(s) 841
BstSCI CCNGG 2 cut(s) 235, 245
BstSFI CTRYAG 1 cut(s) 530
BstUI CGCG 1 cut(s) 548
BstV1I GCAGC 3 cut(s) 273, 309, 481
BsuI GTATCC 1 cut(s) 360
BsuRI GGCC 2 cut(s) 485, 569
BtsCI GGATG 6 cut(s) 110, 418, 520, 663, 722, 952
BtsI GCAGTG 2 cut(s) 485, 539
BtsIMutI CAGTG 4 cut(s) 485, 539, 627, 637
Cac8I GCNNGC 5 cut(s) 7, 329, 582, 586, 1186
CaiI CAGNNNCTG 1 cut(s) 203
CciI TCATGA 2 cut(s) 967, 1105
CfoI GCGC 2 cut(s) 327, 548
Cfr10I RCCGGY 1 cut(s) 27
Cfr13I GGNCC 2 cut(s) 334, 483
Csp6I GTAC 2 cut(s) 31, 740
CspAI ACCGGT 1 cut(s) 27
CviQI GTAC 2 cut(s) 31, 740
DpnI GATC 5 cut(s) 234, 714, 789, 1193, 1521
DpnII GATC 5 cut(s) 232, 712, 787, 1191, 1519
Eam1104I CTCTTC 2 cut(s) 685, 1311
EarI CTCTTC 2 cut(s) 685, 1311
Ecl136II GAGCTC 1 cut(s) 355
Eco147I AGGCCT 1 cut(s) 569
Eco24I GRGCYC 2 cut(s) 357, 811
Eco31I GGTCTC 1 cut(s) 1170
Eco47I GGWCC 1 cut(s) 334
Eco53kI GAGCTC 1 cut(s) 355
Eco57I CTGAAG 1 cut(s) 1029
EcoICRI GAGCTC 1 cut(s) 355
EcoRI GAATTC 1 cut(s) 625
EcoT38I GRGCYC 2 cut(s) 357, 811
FalI AAGNNNNNCTT 2 cut(s) 872, 904
FaqI GGGAC 2 cut(s) 1024, 1271
FauI CCCGC 1 cut(s) 581
FbaI TGATCA 4 cut(s) 712, 787, 1191, 1519
FblI GTMKAC 1 cut(s) 1362
Fnu4HI GCNGC 3 cut(s) 262, 323, 470
FokI GGATG 6 cut(s) 97, 425, 527, 670, 709, 939
FriOI GRGCYC 2 cut(s) 357, 811
Fsp4HI GCNGC 3 cut(s) 262, 323, 470
FspBI CTAG 3 cut(s) 681, 867, 1014
GlaI GCGC 2 cut(s) 326, 547
GluI GCNGC 3 cut(s) 262, 323, 470
HaeIII GGCC 2 cut(s) 485, 569
HapII CCGG 5 cut(s) 10, 28, 236, 247, 332
HhaI GCGC 2 cut(s) 327, 548
Hin6I GCGC 2 cut(s) 325, 546
HinP1I GCGC 2 cut(s) 325, 546
HincII GTYRAC 1 cut(s) 835
HindII GTYRAC 1 cut(s) 835
HindIII AAGCTT 1 cut(s) 86
HinfI GANTC 3 cut(s) 200, 1089, 1102
HpaII CCGG 5 cut(s) 10, 28, 236, 247, 332
HphI GGTGA 5 cut(s) 218, 562, 679, 1420, 1477
Hpy166II GTNNAC 3 cut(s) 835, 1059, 1363
Hpy188I TCNGA 6 cut(s) 63, 205, 295, 657, 1202, 1394
Hpy188III TCNNGA 5 cut(s) 76, 378, 505, 968, 1106
Hpy8I GTNNAC 3 cut(s) 835, 1059, 1363
HpyAV CCTTC 6 cut(s) 199, 488, 664, 773, 1053, 1067
HpyCH4III ACNGT 1 cut(s) 388
HpyF10VI GCNNNNNNNGC 7 cut(s) 270, 307, 328, 449, 475, 545, 905
HspAI GCGC 2 cut(s) 325, 546
Ksp22I TGATCA 4 cut(s) 712, 787, 1191, 1519
Kzo9I GATC 5 cut(s) 232, 712, 787, 1191, 1519
LmnI GCTCC 4 cut(s) 278, 360, 851, 1267
Lsp1109I GCAGC 3 cut(s) 273, 309, 481
LweI GCATC 8 cut(s) 119, 465, 505, 958, 1099, 1228, 1293, 1387
MaeI CTAG 3 cut(s) 681, 867, 1014
MaeIII GTNAC 2 cut(s) 718, 931
MalI GATC 5 cut(s) 234, 714, 789, 1193, 1521
MboI GATC 5 cut(s) 232, 712, 787, 1191, 1519
MboII GAAGA 7 cut(s) 177, 565, 647, 702, 788, 880, 1328
MhlI GDGCHC 3 cut(s) 357, 811, 1438
MmeI TCCRAC 2 cut(s) 983, 1180
MnlI CCTC 5 cut(s) 124, 403, 1078, 1092, 1312
MseI TTAA 6 cut(s) 138, 390, 750, 762, 1250, 1404
MslI CAYNNNNRTG 2 cut(s) 972, 1193
MspI CCGG 5 cut(s) 10, 28, 236, 247, 332
MspR9I CCNGG 2 cut(s) 237, 247
MvnI CGCG 1 cut(s) 548
MwoI GCNNNNNNNGC 7 cut(s) 270, 307, 328, 449, 475, 545, 905
NciI CCSGG 2 cut(s) 237, 247
NdeII GATC 5 cut(s) 232, 712, 787, 1191, 1519
NlaIV GGNNCC 2 cut(s) 1041, 1153
NmuCI GTSAC 1 cut(s) 931
NspI RCATGY 1 cut(s) 841
NspV TTCGAA 1 cut(s) 97
PagI TCATGA 2 cut(s) 967, 1105
PceI AGGCCT 1 cut(s) 569
PfeI GAWTC 3 cut(s) 200, 1089, 1102
PflFI GACNNNGTC 1 cut(s) 54
PinAI ACCGGT 1 cut(s) 27
PkrI GCNGC 3 cut(s) 263, 324, 471
PshBI ATTAAT 1 cut(s) 1404
PsiI TTATAA 2 cut(s) 644, 1386
Psp124BI GAGCTC 1 cut(s) 357
PspN4I GGNNCC 2 cut(s) 1041, 1153
PspPI GGNCC 2 cut(s) 334, 483
PstI CTGCAG 1 cut(s) 534
PstNI CAGNNNCTG 1 cut(s) 203
PsyI GACNNNGTC 1 cut(s) 54
RsaI GTAC 2 cut(s) 32, 741
RsaNI GTAC 2 cut(s) 31, 740
RseI CAYNNNNRTG 2 cut(s) 972, 1193
SacI GAGCTC 1 cut(s) 357
SaqAI TTAA 6 cut(s) 138, 390, 750, 762, 1250, 1404
SatI GCNGC 3 cut(s) 262, 323, 470
Sau3AI GATC 5 cut(s) 232, 712, 787, 1191, 1519
Sau96I GGNCC 2 cut(s) 334, 483
ScrFI CCNGG 2 cut(s) 237, 247
SduI GDGCHC 3 cut(s) 357, 811, 1438
SfaNI GCATC 8 cut(s) 119, 465, 505, 958, 1099, 1228, 1293, 1387
SfcI CTRYAG 1 cut(s) 530
SfuI TTCGAA 1 cut(s) 97
SinI GGWCC 1 cut(s) 334
SmiMI CAYNNNNRTG 2 cut(s) 972, 1193
SpeI ACTAGT 1 cut(s) 866
SseBI AGGCCT 1 cut(s) 569
SsiI CCGC 3 cut(s) 548, 588, 1077
SspI AATATT 1 cut(s) 1473
SspMI CTAG 3 cut(s) 681, 867, 1014
SstI GAGCTC 1 cut(s) 357
StuI AGGCCT 1 cut(s) 569
StyD4I CCNGG 2 cut(s) 235, 245
TaaI ACNGT 1 cut(s) 388
TaqI TCGA 3 cut(s) 97, 210, 1345
TatI WGTACW 1 cut(s) 739
TfiI GAWTC 3 cut(s) 200, 1089, 1102
Tru1I TTAA 6 cut(s) 138, 390, 750, 762, 1250, 1404
Tru9I TTAA 6 cut(s) 138, 390, 750, 762, 1250, 1404
TscAI CASTG 4 cut(s) 485, 539, 627, 637
TseFI GTSAC 1 cut(s) 931
TseI GCWGC 3 cut(s) 261, 322, 469
Tsp45I GTSAC 1 cut(s) 931
TspDTI ATGAA 6 cut(s) 81, 181, 435, 787, 1329, 1491
TspRI CASTG 4 cut(s) 485, 539, 627, 637
Tth111I GACNNNGTC 1 cut(s) 54
VpaK11BI GGWCC 1 cut(s) 334
VspI ATTAAT 1 cut(s) 1404
XapI RAATTY 7 cut(s) 99, 218, 423, 500, 625, 650, 1347
XceI RCATGY 1 cut(s) 841
XmiI GTMKAC 1 cut(s) 1362
XspI CTAG 3 cut(s) 681, 867, 1014
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.