MD15G1064300.v1.1

sodium-coupled neutral amino acid transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000616
Physical Location & Seq
Forward (+)
4461071 .. 4464294
3224 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1064300.v1.1.491

Sequence Viewer

Length: 1368 bp
ATGACGATTCTCTCCTCGGACCGGAAGCAGCGGCGCAACCCCAAGGCCCCGCTGCTGCCACAGAAGCACGACGAAGACTATGGCTCCGTCGAGGCCGGCTTCGAGGGGGCGTCTTTTTCAGGCGCCGTGTTCAACCTCTCGACGACTATCGTCGGTGCCGGGATCATGGCCCTCCCGGCGGCGGTCAAGCAATTGGGCCTGGTTCCGGGCCTGATTATGATCGTCTTGGGCGCGATGCTGACGGAGTCCTCCATCGACATGATCATGCGGTTCACCAGAGCTTCCAAGGCCGCCTCGTACTCCGGCCTTGTCGGCGACGCGTTCGGTGGGCCCGGCCGGACACTCCTCCAGCTCTGCGTCGTCGTCAACAACTTTGGGATGCTCGTCGTCTACATGATCATCATCGGGGACGTGCTGTCGGGGACGTCGTCGGAAGGAGTCCGCCATTCGGGGGTGATAGAAGAATGGTTCGGACAACGTTGGTGGACGGCCCGGTTCTCGCTGCTGCTTCTCACCACGCTGTTTGTTTTGGCTCCCCTTATTTCTTTCAAGCGCGTAGATTCTTTGAGATACACATCAGCGTTGTCCGTTGCTTTGGCTGTTGTATTTGTCGCAATAACTGCGGGAGTAGCAATCTTTAAGTTGATTGATGGAAGCATTGAATTTCCACGTTTGATGCCCAAACTGGTTGACCAGGCATCGTTTTGGAAGCTTTTCACCACCATCCCTATTCTTGTCACCGCCTATATCTGCCACCATAACATTCACCCTATAGAGAACGAACTAAGAGACCCTGCGCAGATGAAGTCAATAGTGCGAACGTCGCTTGCATTTTGCTCAACTGTTTACATCGCAACCAGCTTCTTTGGCTTCCTTCTCTTTGGGGATCATACAATGGATGATGTGCTGGCAAATTTCGATGCTGATCTTGGAATCCCATATAGCTCGTTTCTTGATGATATTGTTAGGGTGAGCTATGGTGTCCACCTGATGCTCGTTTTCCCCATTGTGTTTTTCTCCCTCCGCCTACATTTGGATGGCCTTCTCTTTCCCTTTGCCATTCCTATCGCATATGACAACCGAAGATTCTACTCAGTAACTGCAGTTCTCATGGGTTTTATTTTTCTGGGAGCCAATTTTGTGCCTAGCATCTGGGTTGCCTTTCAGTTCACAGGTGCTACAGCTGCCATCGCCGTTGGTTTCATATTTCCAGCTGCCGTAGCTCTTAGGAACACTAGTGGAATCGCGACGAAGAAGGACAAACTAGTCTCATGGGTGATGATATTTTTAGCAGTCTCCTCGAGCACGGCGGCCATCTCCAGTGACATTTACTGCATTTTCAGCCGTGAGAAAGTCGCTAGAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04724 ko04727 ko04974 map04724 map04727 map04974
Pfam Domains
Protein Families
Aa_trans

Protein Analysis

456

Amino Acids

49.68

Weight (kDa)

8.59

Isoelectric Point (pI)

31.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 37 - 438 2.6e-55 Transmembrane amino acid transporter protein
Trp_Tyr_perm PF03222 38 - 260 3.2e-06 Tryptophan/tyrosine permease family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000616)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G30390 AT3G30390 AT3G30390 AT5G38820 AT5G38820 AT5G38820
fragaria_vesca FvH4_2g32550 FvH4_2g32550 FvH4_2g32550 FvH4_2g32550 FvH4_2g32550 FvH4_2g32550 FvH4_2g32550 FvH4_4g28960 FvH4_6g46060 FvH4_6g46060 FvH4_6g46080
malus_domestica MD09G1077200.v1.1 MD09G1077400.v1.1 MD15G1064300.v1.1 MD16G1053900.v1.1 MD17G1067600.v1.1 MD17G1067800.v1.1
prunus_persica Prupe.1G295800_v2.0.a1 Prupe.1G295800_v2.0.a1 Prupe.1G295800_v2.0.a1 Prupe.1G418300_v2.0.a1 Prupe.3G246600_v2.0.a1 Prupe.3G246800_v2.0.a1 Prupe.3G246900_v2.0.a1
pyrus_communis pycom09g00430 pycom09g00450 pycom15g06100 pycom16g04690 pycom17g06820
rosa_chinensis RchiOBHm_Chr2g0164381 RchiOBHm_Chr2g0164401 RchiOBHm_Chr4g0437101 RchiOBHm_Chr6g0310121
rosa_laevigata RLG00000006468 RLG00000010508 RLG00000021456 RLG00000021458
rosa_multiflora Rmu_co8348867.1_g000001 Rmu_sc0000294.1_g000029 Rmu_sc0009487.1_g000003 Rmu_sc0018759.1_g000001 Rmu_sc0021401.1_g000003 Rmu_ssc0000006.1_g000009
rosa_roxburghii Rroxscaffold_2G00086590 Rroxscaffold_2G00086610 Rroxscaffold_5G00378170 Rroxscaffold_7G00158840
rosa_rugosa Rorug02G0508200 Rorug02G0508300 Rorug04G0298000 Rorug06G0377600
rosa_samantha Rh2AG576200 Rh2AG576400 Rh2BG587200 Rh2BG587400 Rh2CG558000 Rh2CG558200 Rh2DG596900 Rh2DG597200 Rh4AG352800 Rh4BG362300 Rh4CG376500 Rh4DG356300 Rh6AG491600 Rh6BG501000 Rh6CG506000 Rh6DG492100
rosa_wichuraiana Rw2G047720 Rw2G047740 Rw2G047820 Rw2G047840 Rw4G030890 Rw6G042770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1265
AatII GACGTC 1 cut(s) 428
Acc16I TGCGCA 1 cut(s) 798
AccB1I GGYRCC 2 cut(s) 122, 155
AccI GTMKAC 1 cut(s) 390
AccII CGCG 4 cut(s) 233, 320, 555, 1247
AclI AACGTT 1 cut(s) 478
AclWI GGATC 2 cut(s) 170, 894
AcoI YGGCCR 2 cut(s) 334, 1311
AcsI RAATTY 2 cut(s) 662, 913
AcyI GRCGYC 3 cut(s) 110, 123, 425
AfaI GTAC 1 cut(s) 299
AfiI CCNNNNNNNGG 7 cut(s) 21, 178, 181, 205, 448, 451, 1033
AflIII ACRYGT 1 cut(s) 318
AgsI TTSAA 3 cut(s) 133, 550, 662
AhdI GACNNNNNGTC 1 cut(s) 415
AhlI ACTAGT 2 cut(s) 1235, 1264
AjiI CACGTC 1 cut(s) 412
AjnI CCWGG 2 cut(s) 198, 693
Alw21I GWGCWC 1 cut(s) 1307
Alw26I GTCTC 3 cut(s) 783, 1273, 1300
AlwI GGATC 2 cut(s) 170, 894
AlwNI CAGNNNCTG 1 cut(s) 1100
Ama87I CYCGRG 1 cut(s) 1300
ApaI GGGCCC 1 cut(s) 333
ApeKI GCWGC 7 cut(s) 28, 52, 55, 502, 505, 1184, 1214
ApoI RAATTY 2 cut(s) 662, 913
Asp700I GAANNNNTTC 1 cut(s) 713
AspLEI GCGC 5 cut(s) 36, 125, 233, 555, 799
AspS9I GGNCC 8 cut(s) 19, 46, 169, 196, 208, 329, 330, 490
AsuC2I CCSGG 5 cut(s) 160, 176, 207, 333, 493
AsuHPI GGTGA 8 cut(s) 265, 466, 505, 709, 730, 758, 982, 1288
AvaI CYCGRG 1 cut(s) 1300
AvaII GGWCC 1 cut(s) 19
BaeGI GKGCMC 1 cut(s) 333
BanI GGYRCC 2 cut(s) 122, 155
BanII GRGCYC 1 cut(s) 333
BbsI GAAGAC 1 cut(s) 81
Bbv12I GWGCWC 1 cut(s) 1307
BbvI GCAGC 7 cut(s) 39, 40, 42, 489, 492, 1171, 1201
BccI CCATC 6 cut(s) 260, 644, 731, 1031, 1196, 1322
BceAI ACGGC 6 cut(s) 110, 504, 1178, 1202, 1323, 1329
BcgI CGANNNNNNTGC 2 cut(s) 1281, 1315
BciT130I CCWGG 2 cut(s) 200, 695
BclI TGATCA 2 cut(s) 261, 396
BcnI CCSGG 5 cut(s) 160, 176, 207, 333, 493
BcoDI GTCTC 3 cut(s) 783, 1273, 1300
BcuI ACTAGT 2 cut(s) 1235, 1264
BfaI CTAG 4 cut(s) 1146, 1236, 1265, 1359
BfmI CTRYAG 3 cut(s) 771, 1101, 1179
BfoI RGCGCY 1 cut(s) 126
BglI GCCNNNNNGGC 2 cut(s) 176, 312
Bme1390I CCNGG 7 cut(s) 160, 176, 200, 207, 333, 493, 695
Bme18I GGWCC 1 cut(s) 19
BmeRI GACNNNNNGTC 1 cut(s) 415
BmeT110I CYCGRG 1 cut(s) 1300
BmgBI CACGTC 1 cut(s) 412
BmgT120I GGNCC 8 cut(s) 19, 46, 169, 196, 208, 329, 330, 490
BmiI GGNNCC 8 cut(s) 48, 85, 124, 157, 204, 331, 534, 1132
BmrFI CCNGG 7 cut(s) 160, 176, 200, 207, 333, 493, 695
BmsI GCATC 7 cut(s) 225, 369, 666, 707, 910, 981, 1158
BoxI GACNNNNGTC 1 cut(s) 149
BpiI GAAGAC 1 cut(s) 81
BpmI CTGGAG 2 cut(s) 332, 1303
BpuMI CCSGG 5 cut(s) 160, 176, 207, 333, 493
BsaBI GATNNNNATC 4 cut(s) 218, 401, 574, 924
BsaHI GRCGYC 3 cut(s) 110, 123, 425
BsaI GGTCTC 1 cut(s) 783
BsaJI CCNNGG 3 cut(s) 15, 42, 285
BsaWI WCCGGW 1 cut(s) 21
BsaXI ACNNNNNCTCC 2 cut(s) 68, 98
Bsc4I CCNNNNNNNGG 7 cut(s) 21, 178, 181, 205, 448, 451, 1033
Bse118I RCCGGY 1 cut(s) 95
Bse1I ACTGG 2 cut(s) 690, 1320
Bse8I GATNNNNATC 4 cut(s) 218, 401, 574, 924
BseBI CCWGG 2 cut(s) 200, 695
BseDI CCNNGG 3 cut(s) 15, 42, 285
BseGI GGATG 4 cut(s) 384, 723, 904, 1042
BseJI GATNNNNATC 4 cut(s) 218, 401, 574, 924
BseLI CCNNNNNNNGG 7 cut(s) 21, 178, 181, 205, 448, 451, 1033
BseMII CTCAG 1 cut(s) 1107
BseNI ACTGG 2 cut(s) 690, 1320
BseRI GAGGAG 3 cut(s) 4, 335, 1288
BseSI GKGCMC 1 cut(s) 333
BseX3I CGGCCG 1 cut(s) 334
BseXI GCAGC 7 cut(s) 39, 40, 42, 489, 492, 1171, 1201
Bsh1236I CGCG 4 cut(s) 233, 320, 555, 1247
Bsh1285I CGRYCG 1 cut(s) 337
BshNI GGYRCC 2 cut(s) 122, 155
BsiEI CGRYCG 1 cut(s) 337
BsiHKAI GWGCWC 1 cut(s) 1307
BsiHKCI CYCGRG 1 cut(s) 1300
BsiSI CCGG 9 cut(s) 22, 96, 159, 176, 206, 303, 333, 337, 493
BslFI GGGAC 2 cut(s) 422, 436
BslI CCNNNNNNNGG 7 cut(s) 21, 178, 181, 205, 448, 451, 1033
BsmAI GTCTC 3 cut(s) 783, 1273, 1300
BsmFI GGGAC 2 cut(s) 422, 436
Bso31I GGTCTC 1 cut(s) 783
BsoBI CYCGRG 1 cut(s) 1300
Bsp120I GGGCCC 1 cut(s) 329
Bsp1286I GDGCHC 2 cut(s) 333, 1307
Bsp143I GATC 6 cut(s) 162, 219, 261, 396, 886, 925
Bsp68I TCGCGA 1 cut(s) 1247
BspCNI CTCAG 1 cut(s) 1106
BspFNI CGCG 4 cut(s) 233, 320, 555, 1247
BspLI GGNNCC 8 cut(s) 48, 85, 124, 157, 204, 331, 534, 1132
BspMAI CTGCAG 1 cut(s) 1105
BspPI GGATC 2 cut(s) 170, 894
BspT107I GGYRCC 2 cut(s) 122, 155
BspTNI GGTCTC 1 cut(s) 783
BsrFI RCCGGY 1 cut(s) 95
BsrI ACTGG 2 cut(s) 690, 1320
BssAI RCCGGY 1 cut(s) 95
BssECI CCNNGG 3 cut(s) 15, 42, 285
BssMI GATC 6 cut(s) 162, 219, 261, 396, 886, 925
BssNI GRCGYC 3 cut(s) 110, 123, 425
BssT1I CCWWGG 2 cut(s) 42, 285
Bst2UI CCWGG 2 cut(s) 200, 695
Bst4CI ACNGT 1 cut(s) 844
BstACI GRCGYC 3 cut(s) 110, 123, 425
BstAPI GCANNNNNTGC 1 cut(s) 620
BstC8I GCNNGC 3 cut(s) 97, 828, 909
BstDEI CTNAG 3 cut(s) 785, 1093, 1226
BstF5I GGATG 4 cut(s) 384, 723, 904, 1042
BstFNI CGCG 4 cut(s) 233, 320, 555, 1247
BstH2I RGCGCY 1 cut(s) 126
BstHHI GCGC 5 cut(s) 36, 125, 233, 555, 799
BstKTI GATC 6 cut(s) 165, 222, 264, 399, 889, 928
BstMAI GTCTC 3 cut(s) 783, 1273, 1300
BstMBI GATC 6 cut(s) 162, 219, 261, 396, 886, 925
BstMCI CGRYCG 1 cut(s) 337
BstNI CCWGG 2 cut(s) 200, 695
BstPAI GACNNNNGTC 1 cut(s) 149
BstSCI CCNGG 7 cut(s) 158, 174, 198, 205, 331, 491, 693
BstSFI CTRYAG 3 cut(s) 771, 1101, 1179
BstSLI GKGCMC 1 cut(s) 333
BstUI CGCG 4 cut(s) 233, 320, 555, 1247
BstV1I GCAGC 7 cut(s) 39, 40, 42, 489, 492, 1171, 1201
BstV2I GAAGAC 1 cut(s) 81
BstZI CGGCCG 1 cut(s) 334
BtgZI GCGATG 3 cut(s) 248, 835, 1174
BtrI CACGTC 1 cut(s) 412
BtsCI GGATG 4 cut(s) 384, 723, 904, 1042
BtsIMutI CAGTG 1 cut(s) 1327
BtuMI TCGCGA 1 cut(s) 1247
Cac8I GCNNGC 3 cut(s) 97, 828, 909
CaiI CAGNNNCTG 1 cut(s) 1100
CfoI GCGC 5 cut(s) 36, 125, 233, 555, 799
Cfr10I RCCGGY 1 cut(s) 95
Cfr13I GGNCC 8 cut(s) 19, 46, 169, 196, 208, 329, 330, 490
CpoI CGGWCCG 1 cut(s) 19
CseI GACGC 3 cut(s) 99, 326, 346
Csp6I GTAC 1 cut(s) 298
CspCI CAANNNNNGTGG 4 cut(s) 464, 499, 505, 540
CspI CGGWCCG 1 cut(s) 19
CviAII CATG 6 cut(s) 166, 259, 265, 394, 1111, 1272
CviQI GTAC 1 cut(s) 298
DdeI CTNAG 3 cut(s) 785, 1093, 1226
DinI GGCGCC 1 cut(s) 124
DpnI GATC 6 cut(s) 164, 221, 263, 398, 888, 927
DpnII GATC 6 cut(s) 162, 219, 261, 396, 886, 925
DrdI GACNNNNNNGTC 1 cut(s) 1265
DriI GACNNNNNGTC 1 cut(s) 415
DseDI GACNNNNNNGTC 1 cut(s) 1265
EaeI YGGCCR 2 cut(s) 334, 1311
EagI CGGCCG 1 cut(s) 334
Eam1105I GACNNNNNGTC 1 cut(s) 415
EciI GGCGGA 2 cut(s) 431, 1013
EclXI CGGCCG 1 cut(s) 334
Eco130I CCWWGG 2 cut(s) 42, 285
Eco24I GRGCYC 1 cut(s) 333
Eco31I GGTCTC 1 cut(s) 783
Eco47I GGWCC 1 cut(s) 19
Eco52I CGGCCG 1 cut(s) 334
Eco88I CYCGRG 1 cut(s) 1300
EcoO109I RGGNCCY 1 cut(s) 46
EcoRII CCWGG 2 cut(s) 198, 693
EcoT14I CCWWGG 2 cut(s) 42, 285
EcoT38I GRGCYC 1 cut(s) 333
EgeI GGCGCC 1 cut(s) 124
EheI GGCGCC 1 cut(s) 124
ErhI CCWWGG 2 cut(s) 42, 285
FaeI CATG 6 cut(s) 169, 262, 268, 397, 1114, 1275
FaqI GGGAC 2 cut(s) 422, 436
FatI CATG 6 cut(s) 165, 258, 264, 393, 1110, 1271
FauI CCCGC 2 cut(s) 57, 616
FauNDI CATATG 1 cut(s) 1072
FbaI TGATCA 2 cut(s) 261, 396
FblI GTMKAC 1 cut(s) 390
FokI GGATG 4 cut(s) 391, 710, 911, 1049
FriOI GRGCYC 1 cut(s) 333
FspBI CTAG 4 cut(s) 1146, 1236, 1265, 1359
FspI TGCGCA 1 cut(s) 798
GlaI GCGC 5 cut(s) 35, 124, 232, 554, 798
GsuI CTGGAG 2 cut(s) 332, 1303
HaeII RGCGCY 1 cut(s) 126
HapII CCGG 9 cut(s) 22, 96, 159, 176, 206, 303, 333, 337, 493
HgaI GACGC 3 cut(s) 99, 326, 346
HhaI GCGC 5 cut(s) 36, 125, 233, 555, 799
Hin1I GRCGYC 3 cut(s) 110, 123, 425
Hin1II CATG 6 cut(s) 169, 262, 268, 397, 1114, 1275
Hin6I GCGC 5 cut(s) 34, 123, 231, 553, 797
HinP1I GCGC 5 cut(s) 34, 123, 231, 553, 797
HincII GTYRAC 2 cut(s) 367, 691
HindII GTYRAC 2 cut(s) 367, 691
HindIII AAGCTT 1 cut(s) 710
HinfI GANTC 7 cut(s) 7, 245, 438, 560, 933, 1086, 1242
HpaII CCGG 9 cut(s) 22, 96, 159, 176, 206, 303, 333, 337, 493
HphI GGTGA 8 cut(s) 265, 466, 505, 709, 730, 758, 982, 1288
Hpy166II GTNNAC 8 cut(s) 273, 367, 391, 486, 691, 847, 985, 1170
Hpy188I TCNGA 3 cut(s) 19, 433, 473
Hpy188III TCNNGA 3 cut(s) 139, 953, 1246
Hpy8I GTNNAC 8 cut(s) 273, 367, 391, 486, 691, 847, 985, 1170
HpyAV CCTTC 4 cut(s) 428, 884, 1052, 1249
HpyCH4III ACNGT 1 cut(s) 844
HpyCH4IV ACGT 5 cut(s) 411, 425, 478, 670, 821
HpyCH4V TGCA 3 cut(s) 830, 1103, 1335
HpyF3I CTNAG 3 cut(s) 785, 1093, 1226
HpySE526I ACGT 5 cut(s) 411, 425, 478, 670, 821
Hsp92I GRCGYC 3 cut(s) 110, 123, 425
Hsp92II CATG 6 cut(s) 169, 262, 268, 397, 1114, 1275
HspAI GCGC 5 cut(s) 34, 123, 231, 553, 797
KasI GGCGCC 1 cut(s) 122
KroI GCCGGC 1 cut(s) 95
KroNI GCCGGC 1 cut(s) 97
Ksp22I TGATCA 2 cut(s) 261, 396
Kzo9I GATC 6 cut(s) 162, 219, 261, 396, 886, 925
LmnI GCTCC 3 cut(s) 89, 538, 1130
Lsp1109I GCAGC 7 cut(s) 39, 40, 42, 489, 492, 1171, 1201
LweI GCATC 7 cut(s) 225, 369, 666, 707, 910, 981, 1158
MaeI CTAG 4 cut(s) 1146, 1236, 1265, 1359
MaeII ACGT 5 cut(s) 411, 425, 478, 670, 821
MaeIII GTNAC 3 cut(s) 736, 1096, 1322
MalI GATC 6 cut(s) 164, 221, 263, 398, 888, 927
MboI GATC 6 cut(s) 162, 219, 261, 396, 886, 925
MboII GAAGA 4 cut(s) 86, 473, 1095, 1264
MfeI CAATTG 1 cut(s) 191
MhlI GDGCHC 2 cut(s) 333, 1307
MluCI AATT 4 cut(s) 191, 662, 913, 1135
MluI ACGCGT 1 cut(s) 318
Mly113I GGCGCC 1 cut(s) 123
MlyI GAGTC 2 cut(s) 254, 447
MmeI TCCRAC 1 cut(s) 411
MroNI GCCGGC 1 cut(s) 95
MroXI GAANNNNTTC 1 cut(s) 713
MseI TTAA 1 cut(s) 639
MslI CAYNNNNRTG 3 cut(s) 257, 263, 1035
MspA1I CMGCKG 4 cut(s) 31, 52, 1184, 1214
MspI CCGG 9 cut(s) 22, 96, 159, 176, 206, 303, 333, 337, 493
MspR9I CCNGG 7 cut(s) 160, 176, 200, 207, 333, 493, 695
MunI CAATTG 1 cut(s) 191
MvaI CCWGG 2 cut(s) 200, 695
MvnI CGCG 4 cut(s) 233, 320, 555, 1247
NaeI GCCGGC 1 cut(s) 97
NarI GGCGCC 1 cut(s) 123
NciI CCSGG 5 cut(s) 160, 176, 207, 333, 493
NdeI CATATG 1 cut(s) 1072
NdeII GATC 6 cut(s) 162, 219, 261, 396, 886, 925
NgoMIV GCCGGC 1 cut(s) 95
NlaIII CATG 6 cut(s) 169, 262, 268, 397, 1114, 1275
NlaIV GGNNCC 8 cut(s) 48, 85, 124, 157, 204, 331, 534, 1132
NmuCI GTSAC 2 cut(s) 736, 1322
NruI TCGCGA 1 cut(s) 1247
NsbI TGCGCA 1 cut(s) 798
PaeR7I CTCGAG 1 cut(s) 1300
PcsI WCGNNNNNNNCGW 1 cut(s) 425
PdiI GCCGGC 1 cut(s) 97
PdmI GAANNNNTTC 1 cut(s) 713
PfeI GAWTC 5 cut(s) 7, 560, 933, 1086, 1242
PflFI GACNNNGTC 2 cut(s) 244, 427
PleI GAGTC 2 cut(s) 253, 446
PluTI GGCGCC 1 cut(s) 126
PpsI GAGTC 2 cut(s) 253, 446
PshAI GACNNNNGTC 1 cut(s) 149
Psp1406I AACGTT 1 cut(s) 478
Psp6I CCWGG 2 cut(s) 198, 693
PspGI CCWGG 2 cut(s) 198, 693
PspN4I GGNNCC 8 cut(s) 48, 85, 124, 157, 204, 331, 534, 1132
PspOMI GGGCCC 1 cut(s) 329
PspPI GGNCC 8 cut(s) 19, 46, 169, 196, 208, 329, 330, 490
PspXI VCTCGAGB 1 cut(s) 1300
PsrI GAACNNNNNNTAC 2 cut(s) 1089, 1121
PstI CTGCAG 1 cut(s) 1105
PstNI CAGNNNCTG 1 cut(s) 1100
PsyI GACNNNGTC 2 cut(s) 244, 427
PvuII CAGCTG 2 cut(s) 1184, 1214
RruI TCGCGA 1 cut(s) 1247
RsaI GTAC 1 cut(s) 299
RsaNI GTAC 1 cut(s) 298
RseI CAYNNNNRTG 3 cut(s) 257, 263, 1035
Rsr2I CGGWCCG 1 cut(s) 19
RsrII CGGWCCG 1 cut(s) 19
SaqAI TTAA 1 cut(s) 639
Sau3AI GATC 6 cut(s) 162, 219, 261, 396, 886, 925
Sau96I GGNCC 8 cut(s) 19, 46, 169, 196, 208, 329, 330, 490
SchI GAGTC 2 cut(s) 254, 447
ScrFI CCNGG 7 cut(s) 160, 176, 200, 207, 333, 493, 695
SduI GDGCHC 2 cut(s) 333, 1307
SfaNI GCATC 7 cut(s) 225, 369, 666, 707, 910, 981, 1158
SfcI CTRYAG 3 cut(s) 771, 1101, 1179
SfoI GGCGCC 1 cut(s) 124
Sfr274I CTCGAG 1 cut(s) 1300
SinI GGWCC 1 cut(s) 19
SlaI CTCGAG 1 cut(s) 1300
SmiMI CAYNNNNRTG 3 cut(s) 257, 263, 1035
SmlI CTYRAG 1 cut(s) 1300
SmoI CTYRAG 1 cut(s) 1300
SpeI ACTAGT 2 cut(s) 1235, 1264
Sse9I AATT 4 cut(s) 191, 662, 913, 1135
SspDI GGCGCC 1 cut(s) 122
SspMI CTAG 4 cut(s) 1146, 1236, 1265, 1359
StyD4I CCNGG 7 cut(s) 158, 174, 198, 205, 331, 491, 693
StyI CCWWGG 2 cut(s) 42, 285
TaaI ACNGT 1 cut(s) 844
TaiI ACGT 5 cut(s) 414, 428, 481, 673, 824
TaqI TCGA 6 cut(s) 90, 102, 140, 255, 918, 1301
TasI AATT 4 cut(s) 191, 662, 913, 1135
TauI GCSGC 4 cut(s) 34, 182, 293, 1313
TfiI GAWTC 5 cut(s) 7, 560, 933, 1086, 1242
Tru1I TTAA 1 cut(s) 639
Tru9I TTAA 1 cut(s) 639
TscAI CASTG 1 cut(s) 1327
TseFI GTSAC 2 cut(s) 736, 1322
TseI GCWGC 7 cut(s) 28, 52, 55, 502, 505, 1184, 1214
Tsp45I GTSAC 2 cut(s) 736, 1322
TspDTI ATGAA 2 cut(s) 818, 1192
TspGWI ACGGA 3 cut(s) 76, 257, 577
TspRI CASTG 1 cut(s) 1327
Tth111I GACNNNGTC 2 cut(s) 244, 427
VpaK11BI GGWCC 1 cut(s) 19
XapI RAATTY 2 cut(s) 662, 913
XhoI CTCGAG 1 cut(s) 1300
XmiI GTMKAC 1 cut(s) 390
XmnI GAANNNNTTC 1 cut(s) 713
XspI CTAG 4 cut(s) 1146, 1236, 1265, 1359
ZraI GACGTC 1 cut(s) 426
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.