Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0001053

Physical Location & Seq

Genomic Coordinates Reverse (-)

18737740 .. 18741247

Gene Structure 3508 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1229300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2799 bp

ATGTCCTTCCGTAGTTTGAACATCAACATGAATCTAGCCATACTTCTGTTATTTATGGTTTTCTACAAAGGCTCTTCTACAAGTGGATTTAATCCTACTAATGTCACTGCAAGACCTGATGTTGTGAACATCGGCGCGATTTTCACTTTCGATTCTGTCATTGGAAAAATAGCAAAACTCGCAATCACCCTTGCAGTTGAAGATGTCAATTCCAATCCAGAGATTCTCAATGGAACAAAGCTGACACTTAAAATGCAGAACACCAAATCCAGCGACTTTCTAGGCATCATTGAAGCTTTGCAGTTCATGGAGAACGACACGGTGGCCATAATTGGCCCCCAATTCTCAGCAACAGCTCATGTAATATCACACATCGCCGATGAGCTACAAGTCCCTTTACTATCATTTGCAGCAACAGACCCCAATCTGTCTCCAAATCAGTTCCCGTTCTTGGTTCGAACGACGAGGAGTGACCTCTTTGAGATGGCTGCAGTAGCAGACCTTGTTGGCTACTATGAGTGGAGAGATGTCATAGCAATTTACGTAAATGATGATTTCGGGCGGAATGCTATTGCTGCCTTGGGGGACAAGCTATCCAAAAAGCGCTGTAAAATTTCGTACAAGGTACCTCTGAGCCCCAAGGCAACCAAAGATGAAATCAGAAAGGCACTGATTAGTGTATCTTCAATGGAGTCTAGGATTGTGATCCTGCACATTTATACTAGTTGGGGTTTACAAGTGCTCACCGAAGCGCAAAGCCAAATGATGATGTCGAGTCCATATGTGTGGATTGCTACAGATTGGTTTTCCACCATAATAGATTCAGATCCTTCCTTGCCTTTTACAGCAACAGATGACATTCAAGGGGTTCTCACATTGAAAATGTACACGCCAGAATCAGAACTCAAAAACAAGTTTAAATCGCGGTGGAGCAACTTGACTAGCGCAAGGAGGGTGAATGGCTCTTATTTCGGCTTAAATACCTATGGTCTATATGCCTATGATTCTGTATGGCTTCTTGCTCATGCCCTTGACTCGTTTTTCTCTCGTGGAGGAAATATTTCTTTTGCAAATGACTCAAATTTGCACGAATTACGAGGAGGCAAGTTAAACCTTGATGCTTTGAACATGTTTAATGGTGGCAGCCAGCTGCTCCAAAGCATTTTAGAGGTTAATACAACTGGATTAACTGGGCAAATCAAGTTCAATCCAGACGGAAACTTGATTAATCCTGCATTTGAAGTGATCAATGTGATCGGCACAGGGACTAGGACTATTGGTTATTGGTCCAATTCTTCTGGCCTGTCGCTTGATCCACCTGATATGTCTCAAAGGAAGCTGCACTCTAATGGTTCCAGCACTGATACTCAACGATTATACAGCGTCATCTGGCCCGGACAAACAACTCAGAAGCCTCGCGGGTGGGTTTTCCCCGACAACGGAAGAAAGCTCAGGATTGGAGTCCCCAATCGAGTTAGCTACCGCGAGTTTGTAGGCGTAAAAGGCACTGAGTTCACTGGCTACTGCATTGATGTCTTTCAAGCCGCCTTAAACGAGCTGCCATATGGTGTCCCATTCAATTTTATAGCTTTCGGGGATGGTAAAAAAAACCCTGAAAACAATGAACTTCTACACAGGATCCAAATTGGTGAGTTTGATGGTGTGGTGGGAGATATTACAATCACCACTAGCAGAACAAAGGTGGTGGATTTCACACAGCCATATGTTGAGTCTGGTCTAGTTGTGGTTGCTCCAATTAGGAAGATGAATTCTAGTGCTTGGGCATTTTTGAGGCCTTTTACTCCAATGATGTGGGGTGTCACAGGCATTTTCTTCCTGGTTGTGGGAGCAGTTGTTTGGCTTTTGGAGCGTCGGACTAACGAGGATTTTCGAGGCCGTCCAAGAAAACAATTTGTCACAATTATTTGGTTTAGCTTTTCAACTCTGTTCTTCTCCCAGAAAGAAAAAACCGGCAGCACACTTGGTCGATTTGTGCTTATCATATGGCTATTTGTGGTTCTAATACTCAACTCAAGCTACATTGCTAGCCTGACATCAATCCTCACTGTCGAACAGCTTTCTTCACCTGTTAAAGGGATTGAAAGTCTGGCAACCGGCAATGATCGCATTGGTTTCCTAAAGAATTCATTCGCTGAAAACTACTTAACTGATGAGCTAAACATACACAAGTCAAGACTTGTGCCTCTTAACTCCCCAGAAGAATATGAGAAGGCCTTGAAAGATGGTCCTAATGCAGGTGGTGTTGCTGCAGTGATTGATGAGCGTGCATACATGGAGCTCTTCCTCTCCGTTCGATGTGGATATAGTATCGTAGGTCAAGAATTCACCAAAATGGGCTGGGGTTTTGCCTTTCCAAGGGACTCGCCTCTTGCAATTGACATGTCAACCGCGGTGTTGAAACTATCTGAGAAGGGAGATCTTCAAAAGATTCATGACAAATGGCTGATGAAAAGTGCTTGCAGTGCAGAAGGAGCAAAGCAAGCCGTAGACCGCCTTCCGATGAGTAGCTTCTGGGGTCTCTTTCTGCTGAGTGGCACAGCTTGCTTTCTCGCTCTTGTTCTTCATGTTTTTCGCATGGCGCACCAGTACTACAAGCATTATGATTCCGACTGCGAGAGCTCACAAGCAAAACGCCTTCGGTCCTTCGTTTCGTTTGTAAATAAAAGGGAAGAGGAAGTGAAAAGCAGAACCAAGAGGAAGAGGACTGAGAAGGCTTCAAACAAAGTAGTGCATGATCAAGATTGTAGTTCCATTGATGGTTTGGATGAAAGTGTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0002218 GO:0002252 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0003674 GO:0004888 GO:0005215 GO:0005216 GO:0005261 GO:0005262 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0007267 GO:0008066 GO:0008150 GO:0008324 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009682 GO:0009719 GO:0009725 GO:0009753 GO:0009755 GO:0009864 GO:0009867 GO:0009987 GO:0010033 GO:0010243 GO:0015075 GO:0015085 GO:0015267 GO:0015318 GO:0016020 GO:0019722 GO:0019932 GO:0022803 GO:0022838 GO:0022857 GO:0022890 GO:0023052 GO:0030001 GO:0031347 GO:0031349 GO:0032870 GO:0034220 GO:0035556 GO:0038023 GO:0042221 GO:0042742 GO:0043200 GO:0043207 GO:0044464 GO:0045087 GO:0045088 GO:0045089 GO:0046873 GO:0048518 GO:0048583 GO:0048584 GO:0050776 GO:0050778 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0051716 GO:0055085 GO:0060089 GO:0065007 GO:0070588 GO:0070838 GO:0070887 GO:0071229 GO:0071230 GO:0071310 GO:0071395 GO:0071417 GO:0071495 GO:0071944 GO:0072511 GO:0080134 GO:0098542 GO:0098655 GO:0098660 GO:0098662 GO:1901698 GO:1901699 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

933

Amino Acids

103.95

Weight (kDa)

8.37

Isoelectric Point (pI)

41.03

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peripla_BP_6	PF13458	42 - 345	3.3e-11	Periplasmic binding protein
ANF_receptor	PF01094	59 - 420	2.3e-90	Receptor family ligand binding region
SBP_bac_3	PF00497	485 - 823	4.6e-12	Bacterial extracellular solute-binding proteins, family 3
Lig_chan-Glu_bd	PF10613	503 - 584	4.5e-09	Ligated ion channel L-glutamate- and glycine-binding site
Lig_chan	PF00060	604 - 854	2.9e-33	Ligand-gated ion channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0001053)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G51480 AT3G51480 AT3G51480 AT3G51480
fragaria_vesca	FvH4_1g10192 FvH4_1g10210 FvH4_1g10210
malus_domestica	MD15G1229300.v1.1 MD15G1229400.v1.1 MD15G1229500.v1.1 MD15G1229600.v1.1
prunus_persica	Prupe.7G184100_v2.0.a1 Prupe.7G184300_v2.0.a1 Prupe.7G184400_v2.0.a1 Prupe.7G184400_v2.0.a1 Prupe.7G184400_v2.0.a1 Prupe.7G184400_v2.0.a1 Prupe.7G184500_v2.0.a1
pyrus_communis	pycom15g20440 pycom15g20470
rosa_chinensis	RchiOBHm_Chr1g0342071 RchiOBHm_Chr1g0342111 RchiOBHm_Chr1g0342121 RchiOBHm_Chr1g0342221 RchiOBHm_Chr2g0097001 RchiOBHm_Chr2g0097011
rosa_laevigata	RLG00000016693 RLG00000016695 RLG00000016696 RLG00000029615
rosa_multiflora	Rmu_sc0005782.1_g000005 Rmu_sc0006853.1_g000008
rosa_roxburghii	Rroxscaffold_1G00033430 Rroxscaffold_2G00145050 Rroxscaffold_2G00145070 Rroxscaffold_2G00145080 Rroxscaffold_2G00145090 Rroxscaffold_4G00317590 Rroxscaffold_4G00317600
rosa_rugosa	Rorug01G0152800.1 Rorug01G0153000.1 Rorug02G0062800 Rorug02G0063000
rosa_samantha	Rh1AG167500 Rh1AG167800 Rh1BG134100 Rh1BG134200 Rh1CG155400 Rh1DG168300 Rh1DG168400 Rh2AG110600 Rh2BG112400 Rh2BG112500 Rh2CG114500 Rh2CG114600 Rh2DG113700 Rh2DG113800
rosa_wichuraiana	Rw1G013960 Rw2G008560 Rw2G008580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2246
Acc36I	ACCTGC	1 cut(s)	2246
Acc65I	GGTACC	1 cut(s)	625
AccB1I	GGYRCC	1 cut(s)	625
AccI	GTMKAC	1 cut(s)	2508
AccII	CGCG	5 cut(s)	137, 925, 1419, 1485, 2411
AciI	CCGC	8 cut(s)	562, 925, 1419, 1483, 1545, 2409, 2411, 2512
AclWI	GGATC	5 cut(s)	700, 821, 1307, 1633, 1646
AcoI	YGGCCR	1 cut(s)	324
AcsI	RAATTY	5 cut(s)	612, 1081, 1768, 2143, 2342
AfaI	GTAC	4 cut(s)	620, 627, 887, 2609
AfeI	AGCGCT	1 cut(s)	605
AfiI	CCNNNNNNNGG	6 cut(s)	451, 1439, 1843, 2093, 2255, 2376
AflIII	ACRYGT	2 cut(s)	1128, 2400
AhlI	ACTAGT	1 cut(s)	722
AjnI	CCWGG	1 cut(s)	1836
AloI	GAACNNNNNNTCC	2 cut(s)	251, 283
Alw21I	GWGCWC	3 cut(s)	744, 2301, 2642
Alw26I	GTCTC	3 cut(s)	435, 1332, 2543
AlwI	GGATC	5 cut(s)	700, 821, 1307, 1633, 1646
Aor51HI	AGCGCT	1 cut(s)	605
AoxI	GGCC	7 cut(s)	324, 334, 1300, 1391, 1793, 1894, 2232
ApeKI	GCWGC	9 cut(s)	410, 488, 575, 1143, 1150, 1339, 1558, 1974, 2267
ApoI	RAATTY	5 cut(s)	612, 1081, 1768, 2143, 2342
AseI	ATTAAT	1 cut(s)	1227
Asp700I	GAANNNNTTC	2 cut(s)	1060, 2147
Asp718I	GGTACC	1 cut(s)	625
AspLEI	GCGC	5 cut(s)	137, 606, 754, 947, 2602
AspS9I	GGNCC	5 cut(s)	335, 1287, 1392, 2246, 2661
AsuC2I	CCSGG	1 cut(s)	1395
AsuHPI	GGTGA	7 cut(s)	178, 736, 967, 1661, 1675, 2076, 2338
AsuII	TTCGAA	1 cut(s)	457
AsuNHI	GCTAGC	1 cut(s)	2045
AvaII	GGWCC	3 cut(s)	1287, 2246, 2661
BalI	TGGCCA	1 cut(s)	326
BamHI	GGATCC	1 cut(s)	1638
BanI	GGYRCC	1 cut(s)	625
BanII	GRGCYC	3 cut(s)	638, 2301, 2642
BarI	GAAGNNNNNNTAC	2 cut(s)	2499, 2531
BauI	CACGAG	1 cut(s)	1047
Bbv12I	GWGCWC	3 cut(s)	744, 2301, 2642
BbvI	GCAGC	9 cut(s)	422, 475, 562, 1137, 1155, 1326, 1545, 1986, 2254
BccI	CCATC	5 cut(s)	478, 1592, 1652, 2237, 2771
BceAI	ACGGC	2 cut(s)	1881, 2489
BciT130I	CCWGG	1 cut(s)	1838
BclI	TGATCA	2 cut(s)	1245, 2755
BcnI	CCSGG	1 cut(s)	1395
BcoDI	GTCTC	3 cut(s)	435, 1332, 2543
BcuI	ACTAGT	1 cut(s)	722
BfmI	CTRYAG	3 cut(s)	489, 795, 2268
BfoI	RGCGCY	1 cut(s)	607
BfuAI	ACCTGC	1 cut(s)	2246
BglII	AGATCT	1 cut(s)	2437
BmcAI	AGTACT	1 cut(s)	2609
Bme1390I	CCNGG	2 cut(s)	1395, 1838
Bme18I	GGWCC	3 cut(s)	1287, 2246, 2661
BmgT120I	GGNCC	5 cut(s)	335, 1287, 1392, 2246, 2661
BmiI	GGNNCC	4 cut(s)	337, 627, 1354, 1640
BmrFI	CCNGG	2 cut(s)	1395, 1838
BmrI	ACTGGG	1 cut(s)	1200
BmsI	GCATC	2 cut(s)	294, 1108
BmtI	GCTAGC	1 cut(s)	2049
BmuI	ACTGGG	1 cut(s)	1200
Bpu10I	CCTNAGC	1 cut(s)	1451
Bpu14I	TTCGAA	1 cut(s)	457
BpuEI	CTTGAG	1 cut(s)	2017
BpuMI	CCSGG	1 cut(s)	1395
BsaAI	YACGTR	1 cut(s)	544
BsaBI	GATNNNNATC	2 cut(s)	704, 825
BsaI	GGTCTC	1 cut(s)	2543
BsaJI	CCNNGG	4 cut(s)	579, 639, 2375, 2409
BsaXI	ACNNNNNCTCC	2 cut(s)	1839, 1869
Bsc4I	CCNNNNNNNGG	6 cut(s)	451, 1439, 1843, 2093, 2255, 2376
Bse118I	RCCGGY	2 cut(s)	1970, 2114
Bse1I	ACTGG	4 cut(s)	1186, 1195, 1522, 2605
Bse3DI	GCAATG	2 cut(s)	2040, 2125
Bse8I	GATNNNNATC	2 cut(s)	704, 825
BseBI	CCWGG	1 cut(s)	1838
BseDI	CCNNGG	4 cut(s)	579, 639, 2375, 2409
BseGI	GGATG	2 cut(s)	1603, 2791
BseJI	GATNNNNATC	2 cut(s)	704, 825
BseLI	CCNNNNNNNGG	6 cut(s)	451, 1439, 1843, 2093, 2255, 2376
BseMI	GCAATG	2 cut(s)	2040, 2125
BseMII	CTCAG	8 cut(s)	360, 623, 1421, 1465, 1500, 2418, 2540, 2718
BseNI	ACTGG	4 cut(s)	1186, 1195, 1522, 2605
BseRI	GAGGAG	2 cut(s)	481, 1113
BseXI	GCAGC	9 cut(s)	422, 475, 562, 1137, 1155, 1326, 1545, 1986, 2254
BseYI	CCCAGC	1 cut(s)	2358
BsgI	GTGCAG	3 cut(s)	695, 1325, 2505
Bsh1236I	CGCG	5 cut(s)	137, 925, 1419, 1485, 2411
BshFI	GGCC	7 cut(s)	326, 336, 1302, 1393, 1795, 1896, 2234
BshNI	GGYRCC	1 cut(s)	625
BsiHKAI	GWGCWC	3 cut(s)	744, 2301, 2642
BsiSI	CCGG	3 cut(s)	1395, 1971, 2115
BslFI	GGGAC	6 cut(s)	377, 599, 1279, 1448, 1556, 2393
BslI	CCNNNNNNNGG	6 cut(s)	451, 1439, 1843, 2093, 2255, 2376
BsmAI	GTCTC	3 cut(s)	435, 1332, 2543
BsmFI	GGGAC	6 cut(s)	377, 599, 1279, 1448, 1556, 2393
BsmI	GAATGC	1 cut(s)	571
BsnI	GGCC	7 cut(s)	326, 336, 1302, 1393, 1795, 1896, 2234
Bso31I	GGTCTC	1 cut(s)	2543
Bsp119I	TTCGAA	1 cut(s)	457
Bsp1286I	GDGCHC	4 cut(s)	638, 744, 2301, 2642
Bsp1407I	TGTACA	1 cut(s)	885
Bsp143I	GATC	9 cut(s)	705, 826, 1245, 1254, 1312, 1638, 2122, 2437, 2755
BspACI	CCGC	8 cut(s)	562, 925, 1419, 1483, 1545, 2409, 2411, 2512
BspANI	GGCC	7 cut(s)	326, 336, 1302, 1393, 1795, 1896, 2234
BspCNI	CTCAG	8 cut(s)	359, 624, 1420, 1464, 1501, 2419, 2541, 2719
BspFNI	CGCG	5 cut(s)	137, 925, 1419, 1485, 2411
BspHI	TCATGA	1 cut(s)	2452
BspLI	GGNNCC	4 cut(s)	337, 627, 1354, 1640
BspMAI	CTGCAG	2 cut(s)	493, 2272
BspMI	ACCTGC	1 cut(s)	2246
BspOI	GCTAGC	1 cut(s)	2049
BspPI	GGATC	5 cut(s)	700, 821, 1307, 1633, 1646
BspQI	GCTCTTC	2 cut(s)	79, 2306
BspT104I	TTCGAA	1 cut(s)	457
BspT107I	GGYRCC	1 cut(s)	625
BspTNI	GGTCTC	1 cut(s)	2543
BsrDI	GCAATG	2 cut(s)	2040, 2125
BsrFI	RCCGGY	2 cut(s)	1970, 2114
BsrGI	TGTACA	1 cut(s)	885
BsrI	ACTGG	4 cut(s)	1186, 1195, 1522, 2605
BssAI	RCCGGY	2 cut(s)	1970, 2114
BssECI	CCNNGG	4 cut(s)	579, 639, 2375, 2409
BssMI	GATC	9 cut(s)	705, 826, 1245, 1254, 1312, 1638, 2122, 2437, 2755
BssSI	CACGAG	1 cut(s)	1047
BssT1I	CCWWGG	3 cut(s)	579, 639, 2375
Bst2BI	CACGAG	1 cut(s)	1047
Bst2UI	CCWGG	1 cut(s)	1838
Bst4CI	ACNGT	2 cut(s)	322, 2068
Bst6I	CTCTTC	4 cut(s)	79, 2306, 2685, 2714
BstAPI	GCANNNNNTGC	1 cut(s)	2562
BstAUI	TGTACA	1 cut(s)	885
BstBAI	YACGTR	1 cut(s)	544
BstBI	TTCGAA	1 cut(s)	457
BstC8I	GCNNGC	6 cut(s)	1148, 2047, 2286, 2479, 2502, 2563
BstDEI	CTNAG	8 cut(s)	346, 632, 1407, 1451, 1509, 2427, 2549, 2727
BstDSI	CCRYGG	1 cut(s)	2409
BstENI	CCTNNNNNAGG	3 cut(s)	2091, 2253, 2374
BstF5I	GGATG	2 cut(s)	1603, 2791
BstFNI	CGCG	5 cut(s)	137, 925, 1419, 1485, 2411
BstH2I	RGCGCY	1 cut(s)	607
BstHHI	GCGC	5 cut(s)	137, 606, 754, 947, 2602
BstKTI	GATC	9 cut(s)	708, 829, 1248, 1257, 1315, 1641, 2125, 2440, 2758
BstMAI	GTCTC	3 cut(s)	435, 1332, 2543
BstMBI	GATC	9 cut(s)	705, 826, 1245, 1254, 1312, 1638, 2122, 2437, 2755
BstNI	CCWGG	1 cut(s)	1838
BstNSI	RCATGY	2 cut(s)	1132, 2404
BstSCI	CCNGG	2 cut(s)	1393, 1836
BstSFI	CTRYAG	3 cut(s)	489, 795, 2268
BstSNI	TACGTA	1 cut(s)	544
BstUI	CGCG	5 cut(s)	137, 925, 1419, 1485, 2411
BstV1I	GCAGC	9 cut(s)	422, 475, 562, 1137, 1155, 1326, 1545, 1986, 2254
BstX2I	RGATCY	3 cut(s)	826, 1638, 2437
BstXI	CCANNNNNNTGG	2 cut(s)	786, 1812
BstYI	RGATCY	3 cut(s)	826, 1638, 2437
BsuRI	GGCC	7 cut(s)	326, 336, 1302, 1393, 1795, 1896, 2234
BtgI	CCRYGG	1 cut(s)	2409
BtgZI	GCGATG	1 cut(s)	358
BtsCI	GGATG	2 cut(s)	1603, 2791
BtsI	GCAGTG	3 cut(s)	105, 2277, 2488
BtsIMutI	CAGTG	8 cut(s)	105, 668, 1359, 1506, 1515, 2064, 2277, 2488
BveI	ACCTGC	1 cut(s)	2246
Cac8I	GCNNGC	6 cut(s)	1148, 2047, 2286, 2479, 2502, 2563
CciI	TCATGA	1 cut(s)	2452
CfoI	GCGC	5 cut(s)	137, 606, 754, 947, 2602
Cfr10I	RCCGGY	2 cut(s)	1970, 2114
Cfr13I	GGNCC	5 cut(s)	335, 1287, 1392, 2246, 2661
Cfr42I	CCGCGG	1 cut(s)	2412
CseI	GACGC	2 cut(s)	1372, 1859
Csp6I	GTAC	4 cut(s)	619, 626, 886, 2608
CspCI	CAANNNNNGTGG	2 cut(s)	1686, 1721
CviQI	GTAC	4 cut(s)	619, 626, 886, 2608
DdeI	CTNAG	8 cut(s)	346, 632, 1407, 1451, 1509, 2427, 2549, 2727
DpnI	GATC	9 cut(s)	707, 828, 1247, 1256, 1314, 1640, 2124, 2439, 2757
DpnII	GATC	9 cut(s)	705, 826, 1245, 1254, 1312, 1638, 2122, 2437, 2755
DraI	TTTAAA	1 cut(s)	919
EaeI	YGGCCR	1 cut(s)	324
Eam1104I	CTCTTC	4 cut(s)	79, 2306, 2685, 2714
EarI	CTCTTC	4 cut(s)	79, 2306, 2685, 2714
EciI	GGCGGA	1 cut(s)	577
Ecl136II	GAGCTC	2 cut(s)	2299, 2640
Eco105I	TACGTA	1 cut(s)	544
Eco130I	CCWWGG	3 cut(s)	579, 639, 2375
Eco147I	AGGCCT	2 cut(s)	1795, 2234
Eco24I	GRGCYC	3 cut(s)	638, 2301, 2642
Eco31I	GGTCTC	1 cut(s)	2543
Eco47I	GGWCC	3 cut(s)	1287, 2246, 2661
Eco47III	AGCGCT	1 cut(s)	605
Eco53kI	GAGCTC	2 cut(s)	2299, 2640
EcoICRI	GAGCTC	2 cut(s)	2299, 2640
EcoNI	CCTNNNNNAGG	3 cut(s)	2091, 2253, 2374
EcoRI	GAATTC	3 cut(s)	1768, 2143, 2342
EcoRII	CCWGG	1 cut(s)	1836
EcoT14I	CCWWGG	3 cut(s)	579, 639, 2375
EcoT38I	GRGCYC	3 cut(s)	638, 2301, 2642
ErhI	CCWWGG	3 cut(s)	579, 639, 2375
FaqI	GGGAC	6 cut(s)	377, 599, 1279, 1448, 1556, 2393
FauI	CCCGC	1 cut(s)	1412
FauNDI	CATATG	4 cut(s)	781, 1564, 1723, 2003
FbaI	TGATCA	2 cut(s)	1245, 2755
FblI	GTMKAC	1 cut(s)	2508
FokI	GGATG	1 cut(s)	1610
FriOI	GRGCYC	3 cut(s)	638, 2301, 2642
GlaI	GCGC	5 cut(s)	136, 605, 753, 946, 2601
GsaI	CCCAGC	1 cut(s)	2362
HaeII	RGCGCY	1 cut(s)	607
HaeIII	GGCC	7 cut(s)	326, 336, 1302, 1393, 1795, 1896, 2234
HapII	CCGG	3 cut(s)	1395, 1971, 2115
HgaI	GACGC	2 cut(s)	1372, 1859
HhaI	GCGC	5 cut(s)	137, 606, 754, 947, 2602
Hin6I	GCGC	5 cut(s)	135, 604, 752, 945, 2600
HinP1I	GCGC	5 cut(s)	135, 604, 752, 945, 2600
HincII	GTYRAC	1 cut(s)	2406
HindII	GTYRAC	1 cut(s)	2406
HindIII	AAGCTT	1 cut(s)	294
HpaII	CCGG	3 cut(s)	1395, 1971, 2115
HphI	GGTGA	7 cut(s)	178, 736, 967, 1661, 1675, 2076, 2338
Hpy166II	GTNNAC	6 cut(s)	127, 734, 888, 1515, 2406, 2509
Hpy188I	TCNGA	9 cut(s)	633, 662, 826, 901, 1410, 1875, 2428, 2520, 2629
Hpy188III	TCNNGA	7 cut(s)	218, 1211, 1453, 2193, 2339, 2453, 2759
Hpy8I	GTNNAC	6 cut(s)	127, 734, 888, 1515, 2406, 2509
Hpy99I	CGWCG	2 cut(s)	466, 1875
HpyAV	CCTTC	9 cut(s)	16, 840, 2224, 2425, 2483, 2525, 2666, 2674, 2725
HpyCH4III	ACNGT	2 cut(s)	322, 2068
HpyCH4IV	ACGT	1 cut(s)	543
HpyF3I	CTNAG	8 cut(s)	346, 632, 1407, 1451, 1509, 2427, 2549, 2727
HpySE526I	ACGT	1 cut(s)	543
HspAI	GCGC	5 cut(s)	135, 604, 752, 945, 2600
KpnI	GGTACC	1 cut(s)	629
Ksp22I	TGATCA	2 cut(s)	1245, 2755
KspI	CCGCGG	1 cut(s)	2412
Kzo9I	GATC	9 cut(s)	705, 826, 1245, 1254, 1312, 1638, 2122, 2437, 2755
LguI	GCTCTTC	2 cut(s)	79, 2306
LmnI	GCTCC	7 cut(s)	930, 1158, 1756, 1847, 1867, 2296, 2492
Lsp1109I	GCAGC	9 cut(s)	422, 475, 562, 1137, 1155, 1326, 1545, 1986, 2254
LweI	GCATC	2 cut(s)	294, 1108
MaeII	ACGT	1 cut(s)	543
MaeIII	GTNAC	4 cut(s)	103, 470, 1819, 1915
MalI	GATC	9 cut(s)	707, 828, 1247, 1256, 1314, 1640, 2124, 2439, 2757
MboI	GATC	9 cut(s)	705, 826, 1245, 1254, 1312, 1638, 2122, 2437, 2755
MfeI	CAATTG	1 cut(s)	2394
MflI	RGATCY	3 cut(s)	826, 1638, 2437
MhlI	GDGCHC	4 cut(s)	638, 744, 2301, 2642
MlsI	TGGCCA	1 cut(s)	326
MluNI	TGGCCA	1 cut(s)	326
MlyI	GAGTC	7 cut(s)	701, 784, 1028, 1070, 1470, 1739, 2375
MmeI	TCCRAC	2 cut(s)	1853, 2652
Mox20I	TGGCCA	1 cut(s)	326
MroXI	GAANNNNTTC	2 cut(s)	1060, 2147
MscI	TGGCCA	1 cut(s)	326
MslI	CAYNNNNRTG	4 cut(s)	26, 784, 1347, 2351
Msp20I	TGGCCA	1 cut(s)	326
MspA1I	CMGCKG	2 cut(s)	1150, 2411
MspI	CCGG	3 cut(s)	1395, 1971, 2115
MspR9I	CCNGG	2 cut(s)	1395, 1838
MunI	CAATTG	1 cut(s)	2394
Mva1269I	GAATGC	1 cut(s)	571
MvaI	CCWGG	1 cut(s)	1838
MvnI	CGCG	5 cut(s)	137, 925, 1419, 1485, 2411
NciI	CCSGG	1 cut(s)	1395
NdeI	CATATG	4 cut(s)	781, 1564, 1723, 2003
NdeII	GATC	9 cut(s)	705, 826, 1245, 1254, 1312, 1638, 2122, 2437, 2755
NheI	GCTAGC	1 cut(s)	2045
NlaIV	GGNNCC	4 cut(s)	337, 627, 1354, 1640
NmuCI	GTSAC	4 cut(s)	103, 470, 1819, 1915
NspI	RCATGY	2 cut(s)	1132, 2404
NspV	TTCGAA	1 cut(s)	457
PagI	TCATGA	1 cut(s)	2452
PaqCI	CACCTGC	1 cut(s)	2246
PceI	AGGCCT	2 cut(s)	1795, 2234
PciI	ACATGT	2 cut(s)	1128, 2400
PciSI	GCTCTTC	2 cut(s)	79, 2306
PctI	GAATGC	1 cut(s)	571
PdmI	GAANNNNTTC	2 cut(s)	1060, 2147
PfeI	GAWTC	8 cut(s)	31, 152, 223, 821, 896, 1004, 2449, 2624
PleI	GAGTC	7 cut(s)	700, 783, 1028, 1070, 1469, 1738, 2375
PpsI	GAGTC	7 cut(s)	700, 783, 1028, 1070, 1469, 1738, 2375
Ppu21I	YACGTR	1 cut(s)	544
PscI	ACATGT	2 cut(s)	1128, 2400
PshBI	ATTAAT	1 cut(s)	1227
Psp124BI	GAGCTC	2 cut(s)	2301, 2642
Psp6I	CCWGG	1 cut(s)	1836
PspFI	CCCAGC	1 cut(s)	2358
PspGI	CCWGG	1 cut(s)	1836
PspN4I	GGNNCC	4 cut(s)	337, 627, 1354, 1640
PspPI	GGNCC	5 cut(s)	335, 1287, 1392, 2246, 2661
PstI	CTGCAG	2 cut(s)	493, 2272
PsuI	RGATCY	3 cut(s)	826, 1638, 2437
PvuII	CAGCTG	1 cut(s)	1150
RsaI	GTAC	4 cut(s)	620, 627, 887, 2609
RsaNI	GTAC	4 cut(s)	619, 626, 886, 2608
RseI	CAYNNNNRTG	4 cut(s)	26, 784, 1347, 2351
SacI	GAGCTC	2 cut(s)	2301, 2642
SacII	CCGCGG	1 cut(s)	2412
SapI	GCTCTTC	2 cut(s)	79, 2306
Sau3AI	GATC	9 cut(s)	705, 826, 1245, 1254, 1312, 1638, 2122, 2437, 2755
Sau96I	GGNCC	5 cut(s)	335, 1287, 1392, 2246, 2661
ScaI	AGTACT	1 cut(s)	2609
SchI	GAGTC	7 cut(s)	701, 784, 1028, 1070, 1470, 1739, 2375
ScrFI	CCNGG	2 cut(s)	1395, 1838
SduI	GDGCHC	4 cut(s)	638, 744, 2301, 2642
SfaNI	GCATC	2 cut(s)	294, 1108
SfcI	CTRYAG	3 cut(s)	489, 795, 2268
Sfr303I	CCGCGG	1 cut(s)	2412
SfuI	TTCGAA	1 cut(s)	457
SgrBI	CCGCGG	1 cut(s)	2412
SinI	GGWCC	3 cut(s)	1287, 2246, 2661
SmiMI	CAYNNNNRTG	4 cut(s)	26, 784, 1347, 2351
SmlI	CTYRAG	1 cut(s)	2032
SmoI	CTYRAG	1 cut(s)	2032
SnaBI	TACGTA	1 cut(s)	544
SpeI	ACTAGT	1 cut(s)	722
SseBI	AGGCCT	2 cut(s)	1795, 2234
SsiI	CCGC	8 cut(s)	562, 925, 1419, 1483, 1545, 2409, 2411, 2512
SspI	AATATT	1 cut(s)	1060
SstI	GAGCTC	2 cut(s)	2301, 2642
StuI	AGGCCT	2 cut(s)	1795, 2234
StyD4I	CCNGG	2 cut(s)	1393, 1836
StyI	CCWWGG	3 cut(s)	579, 639, 2375
TaaI	ACNGT	2 cut(s)	322, 2068
TaiI	ACGT	1 cut(s)	546
TaqI	TCGA	8 cut(s)	150, 457, 773, 1471, 1891, 1987, 2070, 2314
TaqII	GACCGA	1 cut(s)	2649
TatI	WGTACW	2 cut(s)	885, 2607
TauI	GCSGC	1 cut(s)	1547
TfiI	GAWTC	8 cut(s)	31, 152, 223, 821, 896, 1004, 2449, 2624
TscAI	CASTG	8 cut(s)	112, 675, 1366, 1513, 1522, 2071, 2277, 2488
TseFI	GTSAC	4 cut(s)	103, 470, 1819, 1915
TseI	GCWGC	9 cut(s)	410, 488, 575, 1143, 1150, 1339, 1558, 1974, 2267
Tsp45I	GTSAC	4 cut(s)	103, 470, 1819, 1915
TspDTI	ATGAA	9 cut(s)	44, 295, 669, 1638, 1781, 2136, 2441, 2483, 2573
TspGWI	ACGGA	3 cut(s)	1230, 1455, 2299
TspRI	CASTG	8 cut(s)	112, 675, 1366, 1513, 1522, 2071, 2277, 2488
VpaK11BI	GGWCC	3 cut(s)	1287, 2246, 2661
VspI	ATTAAT	1 cut(s)	1227
XagI	CCTNNNNNAGG	3 cut(s)	2091, 2253, 2374
XapI	RAATTY	5 cut(s)	612, 1081, 1768, 2143, 2342
XceI	RCATGY	2 cut(s)	1132, 2404
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2779
XmiI	GTMKAC	1 cut(s)	2508
XmnI	GAANNNNTTC	2 cut(s)	1060, 2147
ZrmI	AGTACT	1 cut(s)	2609

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.